AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041482.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041482.01.T01 MTR_7g111900 96.532 173 2 3 1 172 1 170 1.49e-114 322
MsG0780041482.01.T01 MTR_7g103200 85.065 154 21 1 1 154 1 152 4.13e-92 265
MsG0780041482.01.T01 MTR_1g084200 71.233 146 42 0 1 146 1 146 4.19e-73 218
MsG0780041482.01.T01 MTR_2g019480 49.254 134 68 0 10 143 14 147 9.15e-49 155
MsG0780041482.01.T01 MTR_2g046150 44.375 160 88 1 2 161 6 164 3.73e-40 134
MsG0780041482.01.T01 MTR_4g122930 50.714 140 65 2 1 138 1 138 6.99e-39 130
MsG0780041482.01.T01 MTR_2g091210 45.528 123 67 0 17 139 12 134 1.06e-33 116
MsG0780041482.01.T01 MTR_2g091215 53.636 110 51 0 28 137 23 132 1.23e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041482.01.T01 AT1G09310 62.222 180 55 2 1 168 1 179 1.00e-69 209
MsG0780041482.01.T01 AT1G56580 63.953 172 51 4 1 167 1 166 1.12e-68 206
MsG0780041482.01.T01 AT5G46230 47.692 130 68 0 12 141 14 143 4.01e-46 148
MsG0780041482.01.T01 AT4G24130 45.185 135 74 0 9 143 13 147 4.14e-39 130
MsG0780041482.01.T01 AT1G30020 43.571 140 77 1 13 150 15 154 8.60e-37 125
MsG0780041482.01.T01 AT5G49600 46.207 145 68 4 3 138 1 144 6.30e-35 120

Find 31 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTCTTTGAGAAGCTCTT+TGG 0.291146 7:+91445689 None:intergenic
TTCAATGGTAAAAGACCATT+TGG 0.295506 7:+91445660 None:intergenic
GTCACTGCTTACGTTGAAAA+TGG 0.305607 7:-91445522 MsG0780041482.01.T01:CDS
ATCACATTCAAAACACCTTC+TGG 0.305889 7:-91445405 MsG0780041482.01.T01:CDS
AACCCAAATCAAAAGCTCTT+TGG 0.308533 7:+91445470 None:intergenic
TCAATTTCAAAAGCAGAAAC+AGG 0.316797 7:+91445366 None:intergenic
TCCATGGTAAACCTCGGATT+TGG 0.319079 7:+91445734 None:intergenic
GTTGGTTACAACAGAGAAAC+AGG 0.372734 7:-91445624 MsG0780041482.01.T01:CDS
ACACACAAATTCGAAAAGAT+TGG 0.384478 7:-91445570 MsG0780041482.01.T01:CDS
ATCAAGTGGTGCTACTGTTA+AGG 0.417389 7:-91445328 MsG0780041482.01.T01:CDS
ACTGTTGCTGCTACTGCTGC+TGG 0.440092 7:-91445282 MsG0780041482.01.T01:CDS
ACTTCGGTAGCATAGGAAAC+AGG 0.457776 7:+91445543 None:intergenic
TATATTGATGATCCACCAAC+TGG 0.471274 7:-91445432 MsG0780041482.01.T01:CDS
CACACAAATTCGAAAAGATT+GGG 0.476488 7:-91445569 MsG0780041482.01.T01:CDS
TTGAAAGACATCACTGAAGT+TGG 0.485594 7:-91445642 MsG0780041482.01.T01:CDS
AAAGAAATCTCTCTCCCAAA+TGG 0.487795 7:-91445675 MsG0780041482.01.T01:CDS
TCAATGGTAAAAGACCATTT+GGG 0.506164 7:+91445661 None:intergenic
CCATGGAGACGAAATGTGTC+AGG 0.515719 7:-91445718 MsG0780041482.01.T01:CDS
ACTTCAGTGATGTCTTTCAA+TGG 0.520310 7:+91445645 None:intergenic
TACTGCTGCTGGTGTTAAAG+AGG 0.555141 7:-91445271 MsG0780041482.01.T01:CDS
CCTGACACATTTCGTCTCCA+TGG 0.557991 7:+91445718 None:intergenic
GAAGAGAAAGAGAAATCAAG+TGG 0.560001 7:-91445342 MsG0780041482.01.T01:CDS
TGTTAAGGAAGCTGCTAACA+AGG 0.566122 7:-91445313 MsG0780041482.01.T01:CDS
TTCGTCTCCATGGTAAACCT+CGG 0.581474 7:+91445728 None:intergenic
TTCAACGTAAGCAGTGACTT+CGG 0.602234 7:+91445527 None:intergenic
CAATTTCAAAAGCAGAAACA+GGG 0.604117 7:+91445367 None:intergenic
AGCAGTGACTTCGGTAGCAT+AGG 0.607903 7:+91445536 None:intergenic
TCCAAATCCGAGGTTTACCA+TGG 0.611352 7:-91445735 MsG0780041482.01.T01:CDS
CAACCCCAAAACGATGTCGT+TGG 0.618459 7:-91445775 None:intergenic
GGTAAGATCAAGAAACTGAA+CGG 0.640251 7:-91445501 MsG0780041482.01.T01:CDS
AGAAATCAAATCCAAATCCG+AGG 0.652928 7:-91445745 MsG0780041482.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACACACAAATTCGAAAAGAT+TGG - Chr7:91445458-91445477 MsG0780041482.01.T01:CDS 30.0%
CAATTTCAAAAGCAGAAACA+GGG + Chr7:91445664-91445683 None:intergenic 30.0%
CACACAAATTCGAAAAGATT+GGG - Chr7:91445459-91445478 MsG0780041482.01.T01:CDS 30.0%
TCAATGGTAAAAGACCATTT+GGG + Chr7:91445370-91445389 None:intergenic 30.0%
TCAATTTCAAAAGCAGAAAC+AGG + Chr7:91445665-91445684 None:intergenic 30.0%
TTCAATGGTAAAAGACCATT+TGG + Chr7:91445371-91445390 None:intergenic 30.0%
! AAACTTCTAAAAAGTCCAGA+AGG + Chr7:91445641-91445660 None:intergenic 30.0%
! TTGAATGTGATTTTACCAGT+TGG + Chr7:91445614-91445633 None:intergenic 30.0%
!! AAACCAAAGAGCTTTTGATT+TGG - Chr7:91445555-91445574 MsG0780041482.01.T01:CDS 30.0%
!!! AACCAAAGAGCTTTTGATTT+GGG - Chr7:91445556-91445575 MsG0780041482.01.T01:CDS 30.0%
AAAGAAATCTCTCTCCCAAA+TGG - Chr7:91445353-91445372 MsG0780041482.01.T01:CDS 35.0%
AACCCAAATCAAAAGCTCTT+TGG + Chr7:91445561-91445580 None:intergenic 35.0%
ACTTCAGTGATGTCTTTCAA+TGG + Chr7:91445386-91445405 None:intergenic 35.0%
AGAAATCAAATCCAAATCCG+AGG - Chr7:91445283-91445302 MsG0780041482.01.T01:CDS 35.0%
ATCACATTCAAAACACCTTC+TGG - Chr7:91445623-91445642 MsG0780041482.01.T01:CDS 35.0%
GAAGAGAAAGAGAAATCAAG+TGG - Chr7:91445686-91445705 MsG0780041482.01.T01:CDS 35.0%
GGTAAGATCAAGAAACTGAA+CGG - Chr7:91445527-91445546 MsG0780041482.01.T01:CDS 35.0%
TTGAAAGACATCACTGAAGT+TGG - Chr7:91445386-91445405 MsG0780041482.01.T01:CDS 35.0%
! AATGTGATTTTACCAGTTGG+TGG + Chr7:91445611-91445630 None:intergenic 35.0%
! GATTTCTTTGAGAAGCTCTT+TGG + Chr7:91445342-91445361 None:intergenic 35.0%
! TATATTGATGATCCACCAAC+TGG - Chr7:91445596-91445615 MsG0780041482.01.T01:CDS 35.0%
!!! ACAGAGAAACAGGTTTTGTT+TGG - Chr7:91445414-91445433 MsG0780041482.01.T01:CDS 35.0%
GTCACTGCTTACGTTGAAAA+TGG - Chr7:91445506-91445525 MsG0780041482.01.T01:CDS 40.0%
GTTGGTTACAACAGAGAAAC+AGG - Chr7:91445404-91445423 MsG0780041482.01.T01:CDS 40.0%
TGTTAAGGAAGCTGCTAACA+AGG - Chr7:91445715-91445734 MsG0780041482.01.T01:CDS 40.0%
TTCAACGTAAGCAGTGACTT+CGG + Chr7:91445504-91445523 None:intergenic 40.0%
!! ATCAAGTGGTGCTACTGTTA+AGG - Chr7:91445700-91445719 MsG0780041482.01.T01:CDS 40.0%
ACTTCGGTAGCATAGGAAAC+AGG + Chr7:91445488-91445507 None:intergenic 45.0%
TCCAAATCCGAGGTTTACCA+TGG - Chr7:91445293-91445312 MsG0780041482.01.T01:CDS 45.0%
TCCATGGTAAACCTCGGATT+TGG + Chr7:91445297-91445316 None:intergenic 45.0%
TTCGTCTCCATGGTAAACCT+CGG + Chr7:91445303-91445322 None:intergenic 45.0%
! TACTGCTGCTGGTGTTAAAG+AGG - Chr7:91445757-91445776 MsG0780041482.01.T01:CDS 45.0%
AGCAGTGACTTCGGTAGCAT+AGG + Chr7:91445495-91445514 None:intergenic 50.0%
CCATGGAGACGAAATGTGTC+AGG - Chr7:91445310-91445329 MsG0780041482.01.T01:CDS 50.0%
CCTGACACATTTCGTCTCCA+TGG + Chr7:91445313-91445332 None:intergenic 50.0%
ACTGTTGCTGCTACTGCTGC+TGG - Chr7:91445746-91445765 MsG0780041482.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 91445266 91445784 91445266 ID=MsG0780041482.01;Name=MsG0780041482.01
Chr7 mRNA 91445266 91445784 91445266 ID=MsG0780041482.01.T01;Parent=MsG0780041482.01;Name=MsG0780041482.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|172
Chr7 exon 91445266 91445784 91445266 ID=MsG0780041482.01.T01:exon:18596;Parent=MsG0780041482.01.T01
Chr7 CDS 91445266 91445784 91445266 ID=MsG0780041482.01.T01:cds;Parent=MsG0780041482.01.T01
Gene Sequence

>MsG0780041482.01.T01

ATGTCGTTGGTAACAGAAGAAATCAAATCCAAATCCGAGGTTTACCATGGAGACGAAATGTGTCAGGTGAAATCCAAAGAGCTTCTCAAAGAAATCTCTCTCCCAAATGGTCTTTTACCATTGAAAGACATCACTGAAGTTGGTTACAACAGAGAAACAGGTTTTGTTTGGCTGAAACAGAAGAAAAGCATAACACACAAATTCGAAAAGATTGGGAAACCTGTTTCCTATGCTACCGAAGTCACTGCTTACGTTGAAAATGGTAAGATCAAGAAACTGAACGGTGTTAAAACCAAAGAGCTTTTGATTTGGGTTACTTTGTGTGATATTTATATTGATGATCCACCAACTGGTAAAATCACATTCAAAACACCTTCTGGACTTTTTAGAAGTTTCCCTGTTTCTGCTTTTGAAATTGAAGAAGAGAAAGAGAAATCAAGTGGTGCTACTGTTAAGGAAGCTGCTAACAAGGTCAATGAAACTGTTGCTGCTACTGCTGCTGGTGTTAAAGAGGTCTAA

Protein sequence

>MsG0780041482.01.T01

MSLVTEEIKSKSEVYHGDEMCQVKSKELLKEISLPNGLLPLKDITEVGYNRETGFVWLKQKKSITHKFEKIGKPVSYATEVTAYVENGKIKKLNGVKTKELLIWVTLCDIYIDDPPTGKITFKTPSGLFRSFPVSAFEIEEEKEKSSGATVKEAANKVNETVAATAAGVKEV*