Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013993.01.T01 | VVW90877.1 | 84 | 125 | 19 | 1 | 3 | 127 | 1 | 124 | 3.95E-68 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013993.01.T01 | P0CC50 | 96.907 | 97 | 3 | 0 | 31 | 127 | 414 | 510 | 6.40E-62 | 200 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013993.01.T01 | A0A5K1HVZ2 | 84.000 | 125 | 19 | 1 | 3 | 127 | 1 | 124 | 1.89e-68 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013889.01 | MsG0380013993.01 | 0.808638 | 2.807021e-50 | 1.613309e-47 |
| MsG0380013915.01 | MsG0380013993.01 | 0.848972 | 4.357416e-60 | 8.136933e-57 |
| MsG0380013947.01 | MsG0380013993.01 | 0.829187 | 5.923991e-55 | 6.018996e-52 |
| MsG0380013949.01 | MsG0380013993.01 | 0.820026 | 8.525104e-53 | 6.669406e-50 |
| MsG0380013958.01 | MsG0380013993.01 | 0.820809 | 5.638013e-53 | 4.507346e-50 |
| MsG0380013980.01 | MsG0380013993.01 | 0.817576 | 3.070075e-52 | 2.244195e-49 |
| MsG0380013993.01 | MsG0380014037.01 | 0.801597 | 8.352995e-49 | 4.004909e-46 |
| MsG0380013993.01 | MsG0680032339.01 | 0.839112 | 1.924719e-57 | 2.631474e-54 |
| MsG0380013993.01 | MsG0880045283.01 | 0.809290 | 2.035286e-50 | 1.190311e-47 |
| MsG0280007041.01 | MsG0380013993.01 | 0.802388 | 5.745423e-49 | 2.810962e-46 |
| MsG0280007042.01 | MsG0380013993.01 | 0.808663 | 2.773223e-50 | 1.594921e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013993.01.T01 | MTR_3g035660 | 98.969 | 97 | 1 | 0 | 31 | 127 | 775 | 871 | 4.97e-62 | 203 |
| MsG0380013993.01.T01 | MTR_0002s0310 | 90.722 | 97 | 9 | 0 | 31 | 127 | 317 | 413 | 1.08e-59 | 189 |
| MsG0380013993.01.T01 | MTR_3g091540 | 100.000 | 33 | 0 | 0 | 41 | 73 | 44 | 76 | 9.94e-16 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013993.01.T01 | ATCG01250 | 92.784 | 97 | 7 | 0 | 31 | 127 | 291 | 387 | 3.57e-61 | 192 |
| MsG0380013993.01.T01 | ATCG00890 | 92.784 | 97 | 7 | 0 | 31 | 127 | 291 | 387 | 3.57e-61 | 192 |
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGAAAACTTCATTTATTC+TGG | 0.226485 | 3:+48593296 | MsG0380013993.01.T01:CDS |
| ATGGAATTGTTTGATCTTAC+AGG | 0.227109 | 3:-48593469 | MsG0380013992.01.T01:intron |
| GCACGTGAGGAGTTATTTCT+TGG | 0.274625 | 3:-48593429 | MsG0380013992.01.T01:intron |
| TCCTGAGCAATTTCAATAAT+GGG | 0.279137 | 3:-48593550 | MsG0380013992.01.T01:intron |
| ATCCTGAGCAATTTCAATAA+TGG | 0.298192 | 3:-48593551 | MsG0380013992.01.T01:intron |
| GCAAGCAGGCCTATATTTCT+TGG | 0.316441 | 3:+48593327 | MsG0380013993.01.T01:CDS |
| CTATATTTCTTGGTTTCAAT+AGG | 0.375317 | 3:+48593337 | MsG0380013993.01.T01:CDS |
| ATGGGGTTCATTGATATTCC+TGG | 0.380234 | 3:-48593532 | MsG0380013992.01.T01:intron |
| TTCTGGTGTGGATGGCAAGC+AGG | 0.432708 | 3:+48593313 | MsG0380013993.01.T01:CDS |
| CTATTGAAACCAAGAAATAT+AGG | 0.436794 | 3:-48593336 | MsG0380013992.01.T01:intron |
| GCCCTATGTCTATTATCCTT+AGG | 0.440840 | 3:+48593245 | MsG0380013993.01.T01:CDS |
| GGAGGTCTTCCTCCACTAGC+AGG | 0.443104 | 3:+48593266 | MsG0380013993.01.T01:CDS |
| CCCCATTATTGAAATTGCTC+AGG | 0.459148 | 3:+48593549 | MsG0380013993.01.T01:CDS |
| TTCATTTATTCTGGTGTGGA+TGG | 0.469314 | 3:+48593305 | MsG0380013993.01.T01:CDS |
| ATAATTAAGTTATTAATGAC+TGG | 0.481516 | 3:+48593400 | MsG0380013993.01.T01:CDS |
| CTCACGTGCGAAATTATAGA+AGG | 0.502645 | 3:+48593443 | MsG0380013993.01.T01:CDS |
| AAACTTCATTTATTCTGGTG+TGG | 0.518432 | 3:+48593301 | MsG0380013993.01.T01:CDS |
| TATAATCATACTCAATTCGA+TGG | 0.542526 | 3:-48593488 | MsG0380013992.01.T01:intron |
| TCCTAAGGATAATAGACATA+GGG | 0.556597 | 3:-48593246 | MsG0380013992.01.T01:intron |
| CTCCTAAGGATAATAGACAT+AGG | 0.558738 | 3:-48593247 | MsG0380013992.01.T01:intron |
| CTTCTATAATTTCGCACGTG+AGG | 0.565900 | 3:-48593442 | MsG0380013992.01.T01:intron |
| TAGTGGAGGAAGACCTCCTA+AGG | 0.577052 | 3:-48593261 | MsG0380013992.01.T01:intron |
| CTATGTCTATTATCCTTAGG+AGG | 0.590927 | 3:+48593248 | MsG0380013993.01.T01:CDS |
| CCTGAGCAATTTCAATAATG+GGG | 0.604713 | 3:-48593549 | MsG0380013992.01.T01:intron |
| GTGATAGCATCTACTATACC+AGG | 0.610688 | 3:+48593514 | MsG0380013993.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATTAAGTTATTAATGAC+TGG | + | Chr3:48593400-48593419 | MsG0380013993.01.T01:CDS | 15.0% |
| ! | CTATATTTCTTGGTTTCAAT+AGG | + | Chr3:48593337-48593356 | MsG0380013993.01.T01:CDS | 25.0% |
| ! | CTATTGAAACCAAGAAATAT+AGG | - | Chr3:48593339-48593358 | MsG0380013992.01.T01:intron | 25.0% |
| ! | TATAATCATACTCAATTCGA+TGG | - | Chr3:48593491-48593510 | MsG0380013992.01.T01:intron | 25.0% |
| !!! | TATACACAAAGATCCTTTTT+TGG | + | Chr3:48593211-48593230 | MsG0380013993.01.T01:CDS | 25.0% |
| AAACTTCATTTATTCTGGTG+TGG | + | Chr3:48593301-48593320 | MsG0380013993.01.T01:CDS | 30.0% | |
| ATCCTGAGCAATTTCAATAA+TGG | - | Chr3:48593554-48593573 | MsG0380013992.01.T01:intron | 30.0% | |
| ATGGAATTGTTTGATCTTAC+AGG | - | Chr3:48593472-48593491 | MsG0380013992.01.T01:intron | 30.0% | |
| TCCTAAGGATAATAGACATA+GGG | - | Chr3:48593249-48593268 | MsG0380013992.01.T01:intron | 30.0% | |
| TCCTGAGCAATTTCAATAAT+GGG | - | Chr3:48593553-48593572 | MsG0380013992.01.T01:intron | 30.0% | |
| TCGGAAAACTTCATTTATTC+TGG | + | Chr3:48593296-48593315 | MsG0380013993.01.T01:CDS | 30.0% | |
| CCTGAGCAATTTCAATAATG+GGG | - | Chr3:48593552-48593571 | MsG0380013992.01.T01:intron | 35.0% | |
| CTATGTCTATTATCCTTAGG+AGG | + | Chr3:48593248-48593267 | MsG0380013993.01.T01:CDS | 35.0% | |
| CTCCTAAGGATAATAGACAT+AGG | - | Chr3:48593250-48593269 | MsG0380013992.01.T01:intron | 35.0% | |
| TTCATTTATTCTGGTGTGGA+TGG | + | Chr3:48593305-48593324 | MsG0380013993.01.T01:CDS | 35.0% | |
| !!! | TCCACTAGCAGGTTTTTTTT+CGG | + | Chr3:48593277-48593296 | MsG0380013993.01.T01:CDS | 35.0% |
| ATGGGGTTCATTGATATTCC+TGG | - | Chr3:48593535-48593554 | MsG0380013992.01.T01:intron | 40.0% | |
| CCCCATTATTGAAATTGCTC+AGG | + | Chr3:48593549-48593568 | MsG0380013993.01.T01:CDS | 40.0% | |
| CTCACGTGCGAAATTATAGA+AGG | + | Chr3:48593443-48593462 | MsG0380013993.01.T01:CDS | 40.0% | |
| CTTCTATAATTTCGCACGTG+AGG | - | Chr3:48593445-48593464 | MsG0380013992.01.T01:intron | 40.0% | |
| GAAAAAAAACCTGCTAGTGG+AGG | - | Chr3:48593278-48593297 | MsG0380013992.01.T01:intron | 40.0% | |
| GCCCTATGTCTATTATCCTT+AGG | + | Chr3:48593245-48593264 | MsG0380013993.01.T01:CDS | 40.0% | |
| GCTAAAGAGAGAGCCAAAAA+AGG | - | Chr3:48593227-48593246 | MsG0380013992.01.T01:intron | 40.0% | |
| GTGATAGCATCTACTATACC+AGG | + | Chr3:48593514-48593533 | MsG0380013993.01.T01:CDS | 40.0% | |
| TCCGAAAAAAAACCTGCTAG+TGG | - | Chr3:48593281-48593300 | MsG0380013992.01.T01:intron | 40.0% | |
| GCAAGCAGGCCTATATTTCT+TGG | + | Chr3:48593327-48593346 | MsG0380013993.01.T01:CDS | 45.0% | |
| !! | GCACGTGAGGAGTTATTTCT+TGG | - | Chr3:48593432-48593451 | MsG0380013992.01.T01:intron | 45.0% |
| TAGTGGAGGAAGACCTCCTA+AGG | - | Chr3:48593264-48593283 | MsG0380013992.01.T01:intron | 50.0% | |
| TTCTGGTGTGGATGGCAAGC+AGG | + | Chr3:48593313-48593332 | MsG0380013993.01.T01:CDS | 55.0% | |
| GGAGGTCTTCCTCCACTAGC+AGG | + | Chr3:48593266-48593285 | MsG0380013993.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48593202 | 48593585 | 48593202 | ID=MsG0380013993.01;Name=MsG0380013993.01 |
| Chr3 | mRNA | 48593202 | 48593585 | 48593202 | ID=MsG0380013993.01.T01;Parent=MsG0380013993.01;Name=MsG0380013993.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|127 |
| Chr3 | exon | 48593202 | 48593585 | 48593202 | ID=MsG0380013993.01.T01:exon:8559;Parent=MsG0380013993.01.T01 |
| Chr3 | CDS | 48593202 | 48593585 | 48593202 | ID=MsG0380013993.01.T01:cds;Parent=MsG0380013993.01.T01 |
| Gene Sequence |
| Protein sequence |
