AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013889.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013889.01.T01 MTR_4g051220 95.402 87 4 0 1 87 100 186 5.38e-57 176
MsG0380013889.01.T01 MTR_0002s0590 66.265 83 28 0 1 83 100 182 5.24e-34 121
MsG0380013889.01.T01 MTR_0477s0040 100.000 42 0 0 90 131 1 42 8.37e-24 87.8
MsG0380013889.01.T01 MTR_4g051213 100.000 42 0 0 90 131 1 42 8.37e-24 87.8
MsG0380013889.01.T01 MTR_8g102790 91.892 37 3 0 90 126 1 37 3.74e-18 72.8
MsG0380013889.01.T01 MTR_1g041800 67.857 56 6 1 90 133 1 56 3.00e-17 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013889.01.T01 ATCG00380 77.011 87 20 0 1 87 100 186 1.25e-41 137

Find 28 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 3:+47523834 MsG0380013889.01.T01:CDS
CGAATAATTCCGACAACTTC+TGG 0.195705 3:+47524540 MsG0380013889.01.T01:CDS
ATGACTAGATCTCGGATAAA+TGG 0.278088 3:+47524471 MsG0380013889.01.T01:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 3:-47523814 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 3:+47523803 MsG0380013889.01.T01:CDS
AATTATTCGTAATAAGATAT+TGG 0.314059 3:-47524526 None:intergenic
ATGGATTGGCAATTCTTCGC+GGG 0.450528 3:-47523774 None:intergenic
TTTATCCGAGATCTAGTCAT+AGG 0.463215 3:-47524468 None:intergenic
TGAGGTTTGCAACGATAACT+TGG 0.468099 3:-47523683 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 3:-47523809 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 3:-47523628 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 3:+47523835 MsG0380013889.01.T01:CDS
GATATTGGCTACAATGGTAA+AGG 0.509030 3:-47524511 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 3:-47523788 None:intergenic
TCTGTGGTTAACTAATTGAC+GGG 0.521261 3:-47523627 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 3:-47523775 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 3:+47523703 MsG0380013889.01.T01:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 3:-47523701 None:intergenic
TAATAAGATATTGGCTACAA+TGG 0.565840 3:-47524517 None:intergenic
CAAGATATTATTACAGCGAA+GGG 0.569002 3:+47523704 MsG0380013889.01.T01:CDS
TGCTACCTATGACTAGATCT+CGG 0.572595 3:+47524463 MsG0380013889.01.T01:CDS
GCATTCACTTATTACAGAGA+TGG 0.579208 3:+47524573 MsG0380013889.01.T01:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 3:-47523620 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 3:-47523793 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 3:-47523771 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 3:-47523773 None:intergenic
CATTAACTAAAACATGTCTG+TGG 0.663264 3:-47523643 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 3:-47523772 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCAAATAATTTTATCTTA+CGG - Chr3:47524335-47524354 None:intergenic 10.0%
!! AATTATTCGTAATAAGATAT+TGG - Chr3:47524529-47524548 None:intergenic 15.0%
!! ATCAAATAATAGATAGTAAA+TGG + Chr3:47523834-47523853 MsG0380013889.01.T01:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG + Chr3:47523835-47523854 MsG0380013889.01.T01:CDS 15.0%
!!! TAAACAATGAAATTTTAGAA+TGG + Chr3:47524438-47524457 MsG0380013889.01.T01:intron 15.0%
!! GTTTATTACAGAAAGAAATT+AGG + Chr3:47524029-47524048 MsG0380013889.01.T01:intron 20.0%
!! TTCTGTAATAAACAAAATAG+TGG - Chr3:47524022-47524041 None:intergenic 20.0%
!! TTTGAAAATTAATGAATTGC+TGG + Chr3:47523862-47523881 MsG0380013889.01.T01:intron 20.0%
! AACATTGATGAACTTGATAA+GGG + Chr3:47524112-47524131 MsG0380013889.01.T01:intron 25.0%
! ACTAATCCTTTATATTGGAA+TGG - Chr3:47523812-47523831 None:intergenic 25.0%
! AGTACGAAATAACGTAAAAA+TGG - Chr3:47524374-47524393 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG + Chr3:47523839-47523858 MsG0380013889.01.T01:intron 25.0%
! GATTGACTAATCCTTTATAT+TGG - Chr3:47523817-47523836 None:intergenic 25.0%
! TAACATTGATGAACTTGATA+AGG + Chr3:47524111-47524130 MsG0380013889.01.T01:intron 25.0%
! TATTGTACAATTCCTTTGTT+TGG + Chr3:47524395-47524414 MsG0380013889.01.T01:intron 25.0%
!! AAATAATTTTATCTTACGGC+AGG - Chr3:47524331-47524350 None:intergenic 25.0%
!! AACAAAATAGTGGAATAAAG+TGG - Chr3:47524012-47524031 None:intergenic 25.0%
!! ACAAAATAGTGGAATAAAGT+GGG - Chr3:47524011-47524030 None:intergenic 25.0%
!! ATTGTACAATTCCTTTGTTT+GGG + Chr3:47524396-47524415 MsG0380013889.01.T01:intron 25.0%
!! TAATAAGATATTGGCTACAA+TGG - Chr3:47524520-47524539 None:intergenic 25.0%
!!! TTAAGAATAATTTTGATCCG+AGG + Chr3:47523933-47523952 MsG0380013889.01.T01:intron 25.0%
AGCAAAAAAAACTTACTTCG+AGG + Chr3:47524305-47524324 MsG0380013889.01.T01:intron 30.0%
CAAGATATTATTACAGCGAA+GGG + Chr3:47523704-47523723 MsG0380013889.01.T01:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG - Chr3:47523646-47523665 None:intergenic 30.0%
CTTGATCCATTCCAATATAA+AGG + Chr3:47523803-47523822 MsG0380013889.01.T01:CDS 30.0%
TAAAATAATCCCCCAAACAA+AGG - Chr3:47524410-47524429 None:intergenic 30.0%
TAATCATTATGTAGGAGAGA+TGG - Chr3:47524218-47524237 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG + Chr3:47523703-47523722 MsG0380013889.01.T01:CDS 30.0%
TCATTATTCCAACTTTGATG+TGG - Chr3:47524065-47524084 None:intergenic 30.0%
TTCGCTGTAATAATATCTTG+AGG - Chr3:47523704-47523723 None:intergenic 30.0%
! TTGTACAATTCCTTTGTTTG+GGG + Chr3:47524397-47524416 MsG0380013889.01.T01:intron 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG + Chr3:47523644-47523663 MsG0380013889.01.T01:CDS 30.0%
ATGACTAGATCTCGGATAAA+TGG + Chr3:47524471-47524490 MsG0380013889.01.T01:CDS 35.0%
CATACATATTCCATTTGCGT+AGG + Chr3:47524261-47524280 MsG0380013889.01.T01:intron 35.0%
GCATTCACTTATTACAGAGA+TGG + Chr3:47524573-47524592 MsG0380013889.01.T01:CDS 35.0%
GTCTGTGGTTAACTAATTGA+CGG - Chr3:47523631-47523650 None:intergenic 35.0%
TAGAGAATCCACATCAAAGT+TGG + Chr3:47524054-47524073 MsG0380013889.01.T01:intron 35.0%
TCTGTGGTTAACTAATTGAC+GGG - Chr3:47523630-47523649 None:intergenic 35.0%
TTTATCCGAGATCTAGTCAT+AGG - Chr3:47524471-47524490 None:intergenic 35.0%
! GATATTGGCTACAATGGTAA+AGG - Chr3:47524514-47524533 None:intergenic 35.0%
! TCTTTTTCTCCAGAAGTTGT+CGG - Chr3:47524552-47524571 None:intergenic 35.0%
! TGTACAATTCCTTTGTTTGG+GGG + Chr3:47524398-47524417 MsG0380013889.01.T01:intron 35.0%
ACCCACGTCTATACATGAAT+GGG - Chr3:47523966-47523985 None:intergenic 40.0%
CGAATAATTCCGACAACTTC+TGG + Chr3:47524540-47524559 MsG0380013889.01.T01:CDS 40.0%
GGAATGGATCAAGAGTCAAA+TGG - Chr3:47523796-47523815 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG - Chr3:47523791-47523810 None:intergenic 40.0%
TACCCACGTCTATACATGAA+TGG - Chr3:47523967-47523986 None:intergenic 40.0%
TCATGTATAGACGTGGGTAT+AGG + Chr3:47523968-47523987 MsG0380013889.01.T01:intron 40.0%
TGAGGTTTGCAACGATAACT+TGG - Chr3:47523686-47523705 None:intergenic 40.0%
TGATGAACTTGATAAGGGTG+CGG + Chr3:47524117-47524136 MsG0380013889.01.T01:intron 40.0%
TGCTACCTATGACTAGATCT+CGG + Chr3:47524463-47524482 MsG0380013889.01.T01:CDS 40.0%
! ATAGTGGAATAAAGTGGGCA+AGG - Chr3:47524006-47524025 None:intergenic 40.0%
! GACAACTTCTGGAGAAAAAG+AGG + Chr3:47524551-47524570 MsG0380013889.01.T01:CDS 40.0%
! GAGGGGCATAATCATTATGT+AGG - Chr3:47524226-47524245 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG - Chr3:47523778-47523797 None:intergenic 40.0%
!! GTAGGCTTTTTGTTTACCGA+GGG - Chr3:47524160-47524179 None:intergenic 40.0%
!! TGTAGGCTTTTTGTTTACCG+AGG - Chr3:47524161-47524180 None:intergenic 40.0%
!! TTAACTAATTGACGGGCTTG+AGG - Chr3:47523623-47523642 None:intergenic 40.0%
ATGCCTCACTATTCACTCGA+GGG - Chr3:47524244-47524263 None:intergenic 45.0%
ATTATGTGCGCCTACGCAAA+TGG - Chr3:47524274-47524293 None:intergenic 45.0%
CATGAATGGGGGAAAATCCT+CGG - Chr3:47523953-47523972 None:intergenic 45.0%
CCACGTCTATACATGAATGG+GGG - Chr3:47523964-47523983 None:intergenic 45.0%
CCCACGTCTATACATGAATG+GGG - Chr3:47523965-47523984 None:intergenic 45.0%
CCCCATTCATGTATAGACGT+GGG + Chr3:47523962-47523981 MsG0380013889.01.T01:intron 45.0%
TATGCCTCACTATTCACTCG+AGG - Chr3:47524245-47524264 None:intergenic 45.0%
! TAGCACTGGAACTGCTATGT+AGG - Chr3:47524178-47524197 None:intergenic 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG - Chr3:47523777-47523796 None:intergenic 45.0%
!!! CTTAATTTAATATTTTAATT+AGG - Chr3:47523918-47523937 None:intergenic 5.0%
AAGAGAGGGATTCGAACCCT+CGG + Chr3:47524141-47524160 MsG0380013889.01.T01:intron 50.0%
CCCCCATTCATGTATAGACG+TGG + Chr3:47523961-47523980 MsG0380013889.01.T01:intron 50.0%
TGCCTCACTATTCACTCGAG+GGG - Chr3:47524243-47524262 None:intergenic 50.0%
! GATAAGGGTGCGGAAAGAGA+GGG + Chr3:47524127-47524146 MsG0380013889.01.T01:intron 50.0%
! TGATAAGGGTGCGGAAAGAG+AGG + Chr3:47524126-47524145 MsG0380013889.01.T01:intron 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG - Chr3:47523776-47523795 None:intergenic 50.0%
AGAGATGGCCGAGTGGTTGA+AGG - Chr3:47524203-47524222 None:intergenic 55.0%
ATGTAGGAGAGATGGCCGAG+TGG - Chr3:47524210-47524229 None:intergenic 55.0%
GATTGGCAATTCTTCGCGGG+GGG - Chr3:47523774-47523793 None:intergenic 55.0%
GTGCTACGCCTTCAACCACT+CGG + Chr3:47524192-47524211 MsG0380013889.01.T01:intron 55.0%
TGCCCCTCGAGTGAATAGTG+AGG + Chr3:47524238-47524257 MsG0380013889.01.T01:intron 55.0%
! AGTGGTTGAAGGCGTAGCAC+TGG - Chr3:47524192-47524211 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG - Chr3:47523775-47523794 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 47523605 47524605 47523605 ID=MsG0380013889.01;Name=MsG0380013889.01
Chr3 mRNA 47523605 47524605 47523605 ID=MsG0380013889.01.T01;Parent=MsG0380013889.01;Name=MsG0380013889.01.T01;_AED=0.47;_eAED=0.51;_QI=0|0|0|1|0|0|2|0|133
Chr3 exon 47523605 47523856 47523605 ID=MsG0380013889.01.T01:exon:26396;Parent=MsG0380013889.01.T01
Chr3 exon 47524456 47524605 47524456 ID=MsG0380013889.01.T01:exon:26397;Parent=MsG0380013889.01.T01
Chr3 CDS 47523605 47523856 47523605 ID=MsG0380013889.01.T01:cds;Parent=MsG0380013889.01.T01
Chr3 CDS 47524456 47524605 47524456 ID=MsG0380013889.01.T01:cds;Parent=MsG0380013889.01.T01
Gene Sequence

>MsG0380013889.01.T01

ATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGAATGGATTGCTACCTATGACTAGATCTCGGATAAATGGAAATTTTATTGATAAGACCTTTACCATTGTAGCCAATATCTTATTACGAATAATTCCGACAACTTCTGGAGAAAAAGAGGCATTCACTTATTACAGAGATGGTGCGATTTGA

Protein sequence

>MsG0380013889.01.T01

MASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWNGLLPMTRSRINGNFIDKTFTIVANILLRIIPTTSGEKEAFTYYRDGAI*