AlfalfaGEDB | MsG0380013915.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013915.01

KEGG: K05573
GO: GO:0006120 GO:0008137 GO:0055114
PFAM: PF00361

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013915.01.T01	RHN59672.1	99.248	133	1	1	133	72	204	1.85E-91	273
MsG0380013915.01.T03	RHN59672.1	99.074	108	1	1	108	97	204	1.25E-71	222
MsG0380013915.01.T04	RHN66424.1	98.214	56	1	1	56	134	189	2.16E-31	117
MsG0380013915.01.T02	RHN59672.1	99.187	123	1	1	123	82	204	2.46E-83	252

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013915.01.T01	P0CD38	96.992	133	4	1	133	378	510	1.30E-88	269
MsG0380013915.01.T02	P0CC50	96.748	123	4	1	123	388	510	1.78E-80	248
MsG0380013915.01.T03	P0CC50	97.222	108	3	1	108	403	510	6.07E-70	220
MsG0380013915.01.T04	P0CC50	96.429	56	2	1	56	455	510	1.05E-31	116

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013915.01.T01	A0A0F6NLF3	100.000	133	0	1	133	360	492	1.01e-88	275
MsG0380013915.01.T02	A0A396I225	99.187	123	1	1	123	82	204	1.17e-83	252
MsG0380013915.01.T04	A0A396IQ02	98.214	56	1	1	56	134	189	1.03e-31	117

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002226.01	MsG0380013915.01	0.847140	1.396316e-59	2.458545e-56
MsG0380013889.01	MsG0380013915.01	0.827596	1.433816e-54	1.390427e-51
MsG0380013915.01	MsG0380013916.01	0.846526	2.054612e-59	3.546770e-56
MsG0380013915.01	MsG0380013917.01	0.835258	1.862786e-56	2.265860e-53
MsG0380013915.01	MsG0380013918.01	0.827083	1.902831e-54	1.818141e-51
MsG0380013915.01	MsG0380013920.01	0.838974	2.089767e-57	2.845169e-54
MsG0380013915.01	MsG0380013930.01	0.803324	3.680738e-49	1.844179e-46
MsG0380013915.01	MsG0380013931.01	0.827124	1.860155e-54	1.779328e-51
MsG0380013915.01	MsG0380013947.01	0.964424	2.610555e-123	1.545930e-117
MsG0380013915.01	MsG0380013948.01	0.838203	3.306980e-57	4.397471e-54
MsG0380013915.01	MsG0380013949.01	0.846271	2.412186e-59	4.129284e-56
MsG0380013915.01	MsG0380013952.01	0.839986	1.141321e-57	1.603261e-54
MsG0380013915.01	MsG0380013959.01	0.809935	1.479582e-50	8.802134e-48
MsG0380013915.01	MsG0380013969.01	0.833950	3.972785e-56	4.646449e-53
MsG0380013915.01	MsG0380013972.01	0.802442	5.599145e-49	2.743061e-46
MsG0380013915.01	MsG0380013980.01	0.805187	1.506459e-49	7.918785e-47
MsG0380013915.01	MsG0380013981.01	0.813491	2.491978e-51	1.629805e-48
MsG0380013915.01	MsG0380013983.01	0.823909	1.074275e-53	9.374303e-51
MsG0380013915.01	MsG0380013993.01	0.848972	4.357416e-60	8.136933e-57
MsG0380013915.01	MsG0380014034.01	0.832019	1.200215e-55	1.325070e-52
MsG0380013915.01	MsG0380014035.01	0.821597	3.709265e-53	3.031042e-50
MsG0380013915.01	MsG0380014057.01	0.805973	1.030398e-49	5.526991e-47
MsG0380013915.01	MsG0480021667.01	0.810899	9.160641e-51	5.590676e-48
MsG0380013915.01	MsG0680032339.01	0.932659	6.110900e-95	4.147307e-90
MsG0380013915.01	MsG0780040316.01	0.829656	4.555979e-55	4.692940e-52
MsG0380013915.01	MsG0780040317.01	0.831465	1.643712e-55	1.785195e-52
MsG0380013915.01	MsG0880044440.01	0.800568	1.355738e-48	6.333511e-46
MsG0280007042.01	MsG0380013915.01	0.807091	5.988131e-50	3.305248e-47
MsG0280010137.01	MsG0380013915.01	0.821631	3.644196e-53	2.980740e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013915.01.T01	MTR_3g035660	99.248	133	1	1	133	739	871	9.48e-89	275
MsG0380013915.01.T01	MTR_0002s0310	92.481	133	10	1	133	281	413	1.00e-70	218
MsG0380013915.01.T01	MTR_3g091540	100.000	33	0	47	79	44	76	2.03e-15	67.4
MsG0380013915.01.T03	MTR_3g035660	99.074	108	1	1	108	764	871	8.74e-70	223
MsG0380013915.01.T03	MTR_0002s0310	90.909	99	9	10	108	315	413	4.46e-61	192
MsG0380013915.01.T03	MTR_3g091540	100.000	33	0	22	54	44	76	1.23e-15	67.0
MsG0380013915.01.T04	MTR_3g035660	98.214	56	1	1	56	816	871	1.41e-32	117
MsG0380013915.01.T04	MTR_0002s0310	89.286	56	6	1	56	358	413	1.85e-30	109
MsG0380013915.01.T02	MTR_3g035660	99.187	123	1	1	123	749	871	7.35e-81	254
MsG0380013915.01.T02	MTR_0002s0310	91.870	123	10	1	123	291	413	1.07e-62	196
MsG0380013915.01.T02	MTR_3g091540	100.000	33	0	37	69	44	76	1.64e-15	67.0

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013915.01.T01	ATCG01250	94.737	133	7	1	133	255	387	2.69e-73	223
MsG0380013915.01.T01	ATCG00890	94.737	133	7	1	133	255	387	2.69e-73	223
MsG0380013915.01.T03	ATCG01250	92.929	99	7	10	108	289	387	2.06e-62	194
MsG0380013915.01.T03	ATCG00890	92.929	99	7	10	108	289	387	2.06e-62	194
MsG0380013915.01.T04	ATCG01250	91.071	56	5	1	56	332	387	9.19e-31	110
MsG0380013915.01.T04	ATCG00890	91.071	56	5	1	56	332	387	9.19e-31	110
MsG0380013915.01.T02	ATCG01250	94.309	123	7	1	123	265	387	4.54e-65	202
MsG0380013915.01.T02	ATCG00890	94.309	123	7	1	123	265	387	4.54e-65	202

Find 26 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGGAAAACTTCATTTATTC+TGG	0.226485	3:-48380532	MsG0380013915.01.T01:CDS
ATGGAATTGTTTGATCTTAC+AGG	0.227109	3:+48380359	None:intergenic
GCACGTGAGGAGTTATTTCT+TGG	0.274625	3:+48380399	None:intergenic
TCCTGAGCAATTTCAATAAT+GGG	0.279137	3:+48380278	None:intergenic
ATCCTGAGCAATTTCAATAA+TGG	0.298192	3:+48380277	None:intergenic
GCAAGCAGGCCTATATTTCT+TGG	0.316441	3:-48380501	MsG0380013915.01.T01:CDS
CTATATTTCTTGGTTTCAAT+AGG	0.375317	3:-48380491	MsG0380013915.01.T01:CDS
ATGGGGTTCATTGATATTCC+TGG	0.380234	3:+48380296	None:intergenic
TTCTGGTGTGGATGGCAAGC+AGG	0.432708	3:-48380515	MsG0380013915.01.T01:CDS
CTATTGAAACCAAGAAATAT+AGG	0.436794	3:+48380492	None:intergenic
GCCCTATGTCTATTATCCTT+AGG	0.440840	3:-48380581	MsG0380013915.01.T01:CDS
GGAGGTCTTCCTCCACTAGC+AGG	0.443104	3:-48380560	MsG0380013915.01.T01:CDS
CCCCATTATTGAAATTGCTC+AGG	0.459148	3:-48380279	MsG0380013915.01.T01:CDS
TTCATTTATTCTGGTGTGGA+TGG	0.469314	3:-48380523	MsG0380013915.01.T01:CDS
ATAATTAAGTTATTAATGAC+TGG	0.481516	3:-48380428	MsG0380013915.01.T01:CDS
CTCACGTGCGAAATTATAGA+AGG	0.502645	3:-48380385	MsG0380013915.01.T01:CDS
AAACTTCATTTATTCTGGTG+TGG	0.518432	3:-48380527	MsG0380013915.01.T01:CDS
GATAACATTCGAGATTATGC+AGG	0.531827	3:-48380641	MsG0380013915.01.T01:CDS
TATAATCATACTCAATTCGA+TGG	0.542526	3:+48380340	None:intergenic
TCCTAAGGATAATAGACATA+GGG	0.556597	3:+48380580	None:intergenic
CTCCTAAGGATAATAGACAT+AGG	0.558738	3:+48380579	None:intergenic
CTTCTATAATTTCGCACGTG+AGG	0.565900	3:+48380386	None:intergenic
TAGTGGAGGAAGACCTCCTA+AGG	0.577052	3:+48380565	None:intergenic
CTATGTCTATTATCCTTAGG+AGG	0.590927	3:-48380578	MsG0380013915.01.T01:CDS
CCTGAGCAATTTCAATAATG+GGG	0.604713	3:+48380279	None:intergenic
GTGATAGCATCTACTATACC+AGG	0.610688	3:-48380314	MsG0380013915.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATTAAGTTATTAATGAC+TGG	-	Chr3:48380481-48380500	MsG0380013915.01.T01:CDS	15.0%
!	CTATATTTCTTGGTTTCAAT+AGG	-	Chr3:48380418-48380437	MsG0380013915.01.T01:CDS	25.0%
!	CTATTGAAACCAAGAAATAT+AGG	+	Chr3:48380420-48380439	None:intergenic	25.0%
!	TATAATCATACTCAATTCGA+TGG	+	Chr3:48380572-48380591	None:intergenic	25.0%
!!!	ATACACAAAAGATCCTTTTT+TGG	-	Chr3:48380294-48380313	MsG0380013915.01.T01:CDS	25.0%
	AAACTTCATTTATTCTGGTG+TGG	-	Chr3:48380382-48380401	MsG0380013915.01.T01:CDS	30.0%
	ATCCTGAGCAATTTCAATAA+TGG	+	Chr3:48380635-48380654	None:intergenic	30.0%
	ATGGAATTGTTTGATCTTAC+AGG	+	Chr3:48380553-48380572	None:intergenic	30.0%
	TCCTAAGGATAATAGACATA+GGG	+	Chr3:48380332-48380351	None:intergenic	30.0%
	TCCTGAGCAATTTCAATAAT+GGG	+	Chr3:48380634-48380653	None:intergenic	30.0%
	TCGGAAAACTTCATTTATTC+TGG	-	Chr3:48380377-48380396	MsG0380013915.01.T01:CDS	30.0%
	CCTGAGCAATTTCAATAATG+GGG	+	Chr3:48380633-48380652	None:intergenic	35.0%
	CTATGTCTATTATCCTTAGG+AGG	-	Chr3:48380331-48380350	MsG0380013915.01.T01:CDS	35.0%
	CTCCTAAGGATAATAGACAT+AGG	+	Chr3:48380333-48380352	None:intergenic	35.0%
	GATAACATTCGAGATTATGC+AGG	-	Chr3:48380268-48380287	MsG0380013915.01.T01:CDS	35.0%
	TTCATTTATTCTGGTGTGGA+TGG	-	Chr3:48380386-48380405	MsG0380013915.01.T01:CDS	35.0%
	ATGGGGTTCATTGATATTCC+TGG	+	Chr3:48380616-48380635	None:intergenic	40.0%
	CCCCATTATTGAAATTGCTC+AGG	-	Chr3:48380630-48380649	MsG0380013915.01.T01:CDS	40.0%
	CTCACGTGCGAAATTATAGA+AGG	-	Chr3:48380524-48380543	MsG0380013915.01.T01:CDS	40.0%
	CTTCTATAATTTCGCACGTG+AGG	+	Chr3:48380526-48380545	None:intergenic	40.0%
	GCCCTATGTCTATTATCCTT+AGG	-	Chr3:48380328-48380347	MsG0380013915.01.T01:CDS	40.0%
	GCTAAAGAGAGAGCCAAAAA+AGG	+	Chr3:48380310-48380329	None:intergenic	40.0%
	GTGATAGCATCTACTATACC+AGG	-	Chr3:48380595-48380614	MsG0380013915.01.T01:CDS	40.0%
	TTTCCGAAAAAACCTGCTAG+TGG	+	Chr3:48380364-48380383	None:intergenic	40.0%
	GCAAGCAGGCCTATATTTCT+TGG	-	Chr3:48380408-48380427	MsG0380013915.01.T01:CDS	45.0%
!!	GCACGTGAGGAGTTATTTCT+TGG	+	Chr3:48380513-48380532	None:intergenic	45.0%
!!!	CCTCCACTAGCAGGTTTTTT+CGG	-	Chr3:48380358-48380377	MsG0380013915.01.T01:CDS	45.0%
	CCGAAAAAACCTGCTAGTGG+AGG	+	Chr3:48380361-48380380	None:intergenic	50.0%
	TAGTGGAGGAAGACCTCCTA+AGG	+	Chr3:48380347-48380366	None:intergenic	50.0%
	TTCTGGTGTGGATGGCAAGC+AGG	-	Chr3:48380394-48380413	MsG0380013915.01.T01:CDS	55.0%
	GGAGGTCTTCCTCCACTAGC+AGG	-	Chr3:48380349-48380368	MsG0380013915.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48380265	48380666	48380265	ID=MsG0380013915.01;Name=MsG0380013915.01
Chr3	mRNA	48380265	48380666	48380265	ID=MsG0380013915.01.T01;Parent=MsG0380013915.01;Name=MsG0380013915.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|133
Chr3	exon	48380265	48380666	48380265	ID=MsG0380013915.01.T01:exon:12281;Parent=MsG0380013915.01.T01
Chr3	CDS	48380265	48380666	48380265	ID=MsG0380013915.01.T01:cds;Parent=MsG0380013915.01.T01
Chr3	mRNA	48380265	48380636	48380265	ID=MsG0380013915.01.T02;Parent=MsG0380013915.01;Name=MsG0380013915.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|123
Chr3	exon	48380265	48380636	48380265	ID=MsG0380013915.01.T02:exon:12282;Parent=MsG0380013915.01.T02
Chr3	CDS	48380265	48380636	48380265	ID=MsG0380013915.01.T02:cds;Parent=MsG0380013915.01.T02
Chr3	mRNA	48380265	48380591	48380265	ID=MsG0380013915.01.T03;Parent=MsG0380013915.01;Name=MsG0380013915.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|108
Chr3	exon	48380265	48380591	48380265	ID=MsG0380013915.01.T03:exon:12283;Parent=MsG0380013915.01.T03
Chr3	CDS	48380265	48380591	48380265	ID=MsG0380013915.01.T03:cds;Parent=MsG0380013915.01.T03
Chr3	mRNA	48380265	48380435	48380265	ID=MsG0380013915.01.T04;Parent=MsG0380013915.01;Name=MsG0380013915.01.T04;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|56
Chr3	exon	48380265	48380435	48380265	ID=MsG0380013915.01.T04:exon:12284;Parent=MsG0380013915.01.T04
Chr3	CDS	48380265	48380435	48380265	ID=MsG0380013915.01.T04:cds;Parent=MsG0380013915.01.T04
Chr3	mRNA	48380443	48380526	48380443	ID=MsG0380013915.01.T05;Parent=MsG0380013915.01;Name=MsG0380013915.01.T05;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|27
Chr3	exon	48380443	48380526	48380443	ID=MsG0380013915.01.T05:exon:12285;Parent=MsG0380013915.01.T05
Chr3	CDS	48380443	48380526	48380443	ID=MsG0380013915.01.T05:cds;Parent=MsG0380013915.01.T05

Gene Sequence

>MsG0380013915.01.T05

ATGGCAAGCAGGCCTATATTTCTTGGTTTCAATAGGACTTATTACAAGCGTTGTTTCTATCTACTATTATCTAAAAATAATTAA

>MsG0380013915.01.T01

ACTGATAACATTCGAGATTATGCAGGATTATACACAAAAGATCCTTTTTTGGCTCTCTCTTTAGCCCTATGTCTATTATCCTTAGGAGGTCTTCCTCCACTAGCAGGTTTTTTCGGAAAACTTCATTTATTCTGGTGTGGATGGCAAGCAGGCCTATATTTCTTGGTTTCAATAGGACTTATTACAAGCGTTGTTTCTATCTACTATTATCTAAAAATAATTAAGTTATTAATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

>MsG0380013915.01.T03

TTATCCTTAGGAGGTCTTCCTCCACTAGCAGGTTTTTTCGGAAAACTTCATTTATTCTGGTGTGGATGGCAAGCAGGCCTATATTTCTTGGTTTCAATAGGACTTATTACAAGCGTTGTTTCTATCTACTATTATCTAAAAATAATTAAGTTATTAATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

>MsG0380013915.01.T04

ATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

>MsG0380013915.01.T02

TACACAAAAGATCCTTTTTTGGCTCTCTCTTTAGCCCTATGTCTATTATCCTTAGGAGGTCTTCCTCCACTAGCAGGTTTTTTCGGAAAACTTCATTTATTCTGGTGTGGATGGCAAGCAGGCCTATATTTCTTGGTTTCAATAGGACTTATTACAAGCGTTGTTTCTATCTACTATTATCTAAAAATAATTAAGTTATTAATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

Protein sequence

>MsG0380013915.01.T05

MASRPIFLGFNRTYYKRCFYLLLSKNN*

>MsG0380013915.01.T01

TDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWCGWQAGLYFLVSIGLITSVVSIYYYLKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*

>MsG0380013915.01.T03

LSLGGLPPLAGFFGKLHLFWCGWQAGLYFLVSIGLITSVVSIYYYLKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*

>MsG0380013915.01.T04

MTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*

>MsG0380013915.01.T02

YTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWCGWQAGLYFLVSIGLITSVVSIYYYLKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*