AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680032339.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032339.01.T01 MTR_3g035660 98.039 204 4 0 1 204 668 871 1.31e-138 409
MsG0680032339.01.T01 MTR_0002s0310 92.647 204 15 0 1 204 210 413 7.95e-108 315
MsG0680032339.01.T01 MTR_3g091540 96.970 33 1 0 118 150 44 76 2.08e-13 63.9
MsG0680032339.01.T04 MTR_3g035660 98.667 150 2 0 1 150 722 871 2.37e-100 306
MsG0680032339.01.T04 MTR_0002s0310 92.667 150 11 0 1 150 264 413 8.97e-83 249
MsG0680032339.01.T04 MTR_3g091540 96.970 33 1 0 64 96 44 76 5.26e-14 64.3
MsG0680032339.01.T03 MTR_3g035660 98.750 160 2 0 1 160 712 871 1.07e-107 326
MsG0680032339.01.T03 MTR_0002s0310 93.125 160 11 0 1 160 254 413 7.84e-90 268
MsG0680032339.01.T03 MTR_3g091540 96.970 33 1 0 74 106 44 76 8.01e-14 63.9
MsG0680032339.01.T02 MTR_3g035660 97.906 191 4 0 1 191 681 871 1.64e-129 384
MsG0680032339.01.T02 MTR_0002s0310 93.194 191 13 0 1 191 223 413 1.33e-99 294
MsG0680032339.01.T02 MTR_3g091540 96.970 33 1 0 105 137 44 76 1.74e-13 63.9
MsG0680032339.01.T05 MTR_3g035660 98.214 56 1 0 1 56 816 871 1.41e-32 117
MsG0680032339.01.T05 MTR_0002s0310 89.286 56 6 0 1 56 358 413 1.85e-30 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032339.01.T01 ATCG01250 93.627 204 13 0 1 204 184 387 1.61e-110 322
MsG0680032339.01.T01 ATCG00890 93.627 204 13 0 1 204 184 387 1.61e-110 322
MsG0680032339.01.T01 AT2G07689 38.235 136 82 2 1 136 55 188 4.06e-21 87.4
MsG0680032339.01.T04 ATCG01250 94.000 150 9 0 1 150 238 387 4.78e-85 254
MsG0680032339.01.T04 ATCG00890 94.000 150 9 0 1 150 238 387 4.78e-85 254
MsG0680032339.01.T03 ATCG01250 93.750 160 10 0 1 160 228 387 1.01e-91 272
MsG0680032339.01.T03 ATCG00890 93.750 160 10 0 1 160 228 387 1.01e-91 272
MsG0680032339.01.T02 ATCG01250 94.241 191 11 0 1 191 197 387 2.18e-102 300
MsG0680032339.01.T02 ATCG00890 94.241 191 11 0 1 191 197 387 2.18e-102 300
MsG0680032339.01.T02 AT2G07689 36.585 123 76 2 1 123 68 188 2.21e-16 74.3
MsG0680032339.01.T05 ATCG01250 91.071 56 5 0 1 56 332 387 9.19e-31 110
MsG0680032339.01.T05 ATCG00890 91.071 56 5 0 1 56 332 387 9.19e-31 110

Find 41 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATTCTTAGCATGCTATT+AGG 0.213688 6:+38821798 None:intergenic
TTTGCTTGCATTGTGTTATT+TGG 0.223841 6:+38821987 MsG0680032339.01.T01:CDS
TCGGAAAACTTCATTTATTC+TGG 0.226485 6:+38822135 MsG0680032339.01.T01:CDS
ATGGAATTGTTTGATCTTAC+AGG 0.227109 6:-38822308 None:intergenic
CAAATTGGATATGTAGTTAT+TGG 0.246161 6:+38821882 MsG0680032339.01.T01:CDS
GCACGTGAGGAGTTATTTCT+TGG 0.274625 6:-38822268 None:intergenic
TCCTGAGCAATTTCAATAAT+GGG 0.279137 6:-38822389 None:intergenic
ATCCTGAGCAATTTCAATAA+TGG 0.298192 6:-38822390 None:intergenic
CTATTCTTAGCATGCTATTA+GGG 0.301257 6:+38821799 None:intergenic
TATTCGTCCATAGGTCAAAT+TGG 0.303171 6:+38821867 MsG0680032339.01.T01:CDS
GCAAGCAGGCCTATATTTCT+TGG 0.316441 6:+38822166 MsG0680032339.01.T01:CDS
GCATACGTTTCATGCTTGTT+TGG 0.326865 6:-38821839 None:intergenic
CATACGTTTCATGCTTGTTT+GGG 0.369828 6:-38821838 None:intergenic
CTATATTTCTTGGTTTCAAT+AGG 0.375317 6:+38822176 MsG0680032339.01.T01:CDS
ATGGGGTTCATTGATATTCC+TGG 0.380234 6:-38822371 None:intergenic
GTAGTTATTGGAATAATTGT+TGG 0.390815 6:+38821894 MsG0680032339.01.T01:CDS
GTGTTATTTGGTCTACGTAC+TGG 0.413420 6:+38821999 MsG0680032339.01.T01:CDS
TTCTGGTGTGGATGGCAAGC+AGG 0.432708 6:+38822152 MsG0680032339.01.T01:CDS
CTATTGAAACCAAGAAATAT+AGG 0.436794 6:-38822175 None:intergenic
GCCCTATGTCTATTATCCTT+AGG 0.440840 6:+38822086 MsG0680032339.01.T01:CDS
GGAGGTCTTCCTCCACTAGC+AGG 0.443104 6:+38822107 MsG0680032339.01.T01:CDS
TACATATCCAATTTGACCTA+TGG 0.448161 6:-38821874 None:intergenic
AGCAAAAGTTCCTAGATTCA+TGG 0.448640 6:-38821970 None:intergenic
CCCCATTATTGAAATTGCTC+AGG 0.459148 6:+38822388 MsG0680032339.01.T01:CDS
TTCTATATCTCCATGAATCT+AGG 0.460861 6:+38821960 MsG0680032339.01.T01:CDS
TTCATTTATTCTGGTGTGGA+TGG 0.469314 6:+38822144 MsG0680032339.01.T01:CDS
ATAATTAAGTTATTAATGAC+TGG 0.481516 6:+38822239 MsG0680032339.01.T01:CDS
CTCACGTGCGAAATTATAGA+AGG 0.502645 6:+38822282 MsG0680032339.01.T01:CDS
ATGCTTGCTTATTCGTCCAT+AGG 0.517225 6:+38821858 MsG0680032339.01.T01:CDS
AAACTTCATTTATTCTGGTG+TGG 0.518432 6:+38822140 MsG0680032339.01.T01:CDS
GATAACATTCGAGATTATGC+AGG 0.531827 6:+38822026 MsG0680032339.01.T01:CDS
TATAATCATACTCAATTCGA+TGG 0.542526 6:-38822327 None:intergenic
ATAATTGTTGGAGACTCAAA+TGG 0.548093 6:+38821906 MsG0680032339.01.T01:CDS
TCCTAAGGATAATAGACATA+GGG 0.556597 6:-38822087 None:intergenic
CTCCTAAGGATAATAGACAT+AGG 0.558738 6:-38822088 None:intergenic
CTTCTATAATTTCGCACGTG+AGG 0.565900 6:-38822281 None:intergenic
TAGTGGAGGAAGACCTCCTA+AGG 0.577052 6:-38822102 None:intergenic
CTATGTCTATTATCCTTAGG+AGG 0.590927 6:+38822089 MsG0680032339.01.T01:CDS
CCTGAGCAATTTCAATAATG+GGG 0.604713 6:-38822388 None:intergenic
GTGATAGCATCTACTATACC+AGG 0.610688 6:+38822353 MsG0680032339.01.T01:CDS
ATTGTTGGAGACTCAAATGG+TGG 0.626325 6:+38821909 MsG0680032339.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTAAGTTATTAATGAC+TGG + Chr6:38822239-38822258 MsG0680032339.01.T01:CDS 15.0%
! CAAATTGGATATGTAGTTAT+TGG + Chr6:38821882-38821901 MsG0680032339.01.T01:CDS 25.0%
! CTATATTTCTTGGTTTCAAT+AGG + Chr6:38822176-38822195 MsG0680032339.01.T01:CDS 25.0%
! CTATTGAAACCAAGAAATAT+AGG - Chr6:38822178-38822197 None:intergenic 25.0%
! GTAGTTATTGGAATAATTGT+TGG + Chr6:38821894-38821913 MsG0680032339.01.T01:CDS 25.0%
! TATAATCATACTCAATTCGA+TGG - Chr6:38822330-38822349 None:intergenic 25.0%
!!! ATACACAAAAGATCCTTTTT+TGG + Chr6:38822052-38822071 MsG0680032339.01.T01:CDS 25.0%
AAACTTCATTTATTCTGGTG+TGG + Chr6:38822140-38822159 MsG0680032339.01.T01:CDS 30.0%
ATAATTGTTGGAGACTCAAA+TGG + Chr6:38821906-38821925 MsG0680032339.01.T01:CDS 30.0%
ATCCTGAGCAATTTCAATAA+TGG - Chr6:38822393-38822412 None:intergenic 30.0%
ATGGAATTGTTTGATCTTAC+AGG - Chr6:38822311-38822330 None:intergenic 30.0%
TACATATCCAATTTGACCTA+TGG - Chr6:38821877-38821896 None:intergenic 30.0%
TCCTAAGGATAATAGACATA+GGG - Chr6:38822090-38822109 None:intergenic 30.0%
TCCTGAGCAATTTCAATAAT+GGG - Chr6:38822392-38822411 None:intergenic 30.0%
TCGGAAAACTTCATTTATTC+TGG + Chr6:38822135-38822154 MsG0680032339.01.T01:CDS 30.0%
TTCTATATCTCCATGAATCT+AGG + Chr6:38821960-38821979 MsG0680032339.01.T01:CDS 30.0%
! TTTGCTTGCATTGTGTTATT+TGG + Chr6:38821987-38822006 MsG0680032339.01.T01:CDS 30.0%
AGCAAAAGTTCCTAGATTCA+TGG - Chr6:38821973-38821992 None:intergenic 35.0%
CATACGTTTCATGCTTGTTT+GGG - Chr6:38821841-38821860 None:intergenic 35.0%
CCTGAGCAATTTCAATAATG+GGG - Chr6:38822391-38822410 None:intergenic 35.0%
CTATGTCTATTATCCTTAGG+AGG + Chr6:38822089-38822108 MsG0680032339.01.T01:CDS 35.0%
CTCCTAAGGATAATAGACAT+AGG - Chr6:38822091-38822110 None:intergenic 35.0%
GATAACATTCGAGATTATGC+AGG + Chr6:38822026-38822045 MsG0680032339.01.T01:CDS 35.0%
TATTCGTCCATAGGTCAAAT+TGG + Chr6:38821867-38821886 MsG0680032339.01.T01:CDS 35.0%
TTCATTTATTCTGGTGTGGA+TGG + Chr6:38822144-38822163 MsG0680032339.01.T01:CDS 35.0%
ATGCTTGCTTATTCGTCCAT+AGG + Chr6:38821858-38821877 MsG0680032339.01.T01:CDS 40.0%
ATGGGGTTCATTGATATTCC+TGG - Chr6:38822374-38822393 None:intergenic 40.0%
ATTGTTGGAGACTCAAATGG+TGG + Chr6:38821909-38821928 MsG0680032339.01.T01:CDS 40.0%
CCCCATTATTGAAATTGCTC+AGG + Chr6:38822388-38822407 MsG0680032339.01.T01:CDS 40.0%
CTCACGTGCGAAATTATAGA+AGG + Chr6:38822282-38822301 MsG0680032339.01.T01:CDS 40.0%
CTTCTATAATTTCGCACGTG+AGG - Chr6:38822284-38822303 None:intergenic 40.0%
GCATACGTTTCATGCTTGTT+TGG - Chr6:38821842-38821861 None:intergenic 40.0%
GCCCTATGTCTATTATCCTT+AGG + Chr6:38822086-38822105 MsG0680032339.01.T01:CDS 40.0%
GCTAAAGAGAGAGCCAAAAA+AGG - Chr6:38822068-38822087 None:intergenic 40.0%
GTGATAGCATCTACTATACC+AGG + Chr6:38822353-38822372 MsG0680032339.01.T01:CDS 40.0%
TTTCCGAAAAAACCTGCTAG+TGG - Chr6:38822122-38822141 None:intergenic 40.0%
!! GTGTTATTTGGTCTACGTAC+TGG + Chr6:38821999-38822018 MsG0680032339.01.T01:CDS 40.0%
GCAAGCAGGCCTATATTTCT+TGG + Chr6:38822166-38822185 MsG0680032339.01.T01:CDS 45.0%
!! GCACGTGAGGAGTTATTTCT+TGG - Chr6:38822271-38822290 None:intergenic 45.0%
!!! CCTCCACTAGCAGGTTTTTT+CGG + Chr6:38822116-38822135 MsG0680032339.01.T01:CDS 45.0%
!!! ATTAATAACTTAATTATTTT+TGG - Chr6:38822236-38822255 None:intergenic 5.0%
CCGAAAAAACCTGCTAGTGG+AGG - Chr6:38822119-38822138 None:intergenic 50.0%
TAGTGGAGGAAGACCTCCTA+AGG - Chr6:38822105-38822124 None:intergenic 50.0%
TTCTGGTGTGGATGGCAAGC+AGG + Chr6:38822152-38822171 MsG0680032339.01.T01:CDS 55.0%
GGAGGTCTTCCTCCACTAGC+AGG + Chr6:38822107-38822126 MsG0680032339.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 38821810 38822424 38821810 ID=MsG0680032339.01;Name=MsG0680032339.01
Chr6 mRNA 38821810 38822424 38821810 ID=MsG0680032339.01.T01;Parent=MsG0680032339.01;Name=MsG0680032339.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|204
Chr6 exon 38821810 38822424 38821810 ID=MsG0680032339.01.T01:exon:21148;Parent=MsG0680032339.01.T01
Chr6 CDS 38821810 38822424 38821810 ID=MsG0680032339.01.T01:cds;Parent=MsG0680032339.01.T01
Chr6 mRNA 38821849 38822424 38821849 ID=MsG0680032339.01.T02;Parent=MsG0680032339.01;Name=MsG0680032339.01.T02;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|0|1|0|191
Chr6 exon 38821849 38822424 38821849 ID=MsG0680032339.01.T02:exon:21149;Parent=MsG0680032339.01.T02
Chr6 CDS 38821849 38822424 38821849 ID=MsG0680032339.01.T02:cds;Parent=MsG0680032339.01.T02
Chr6 mRNA 38821942 38822424 38821942 ID=MsG0680032339.01.T03;Parent=MsG0680032339.01;Name=MsG0680032339.01.T03;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|0|1|0|160
Chr6 exon 38821942 38822424 38821942 ID=MsG0680032339.01.T03:exon:21150;Parent=MsG0680032339.01.T03
Chr6 CDS 38821942 38822424 38821942 ID=MsG0680032339.01.T03:cds;Parent=MsG0680032339.01.T03
Chr6 mRNA 38821972 38822424 38821972 ID=MsG0680032339.01.T04;Parent=MsG0680032339.01;Name=MsG0680032339.01.T04;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|150
Chr6 exon 38821972 38822424 38821972 ID=MsG0680032339.01.T04:exon:21151;Parent=MsG0680032339.01.T04
Chr6 CDS 38821972 38822424 38821972 ID=MsG0680032339.01.T04:cds;Parent=MsG0680032339.01.T04
Chr6 mRNA 38822254 38822424 38822254 ID=MsG0680032339.01.T05;Parent=MsG0680032339.01;Name=MsG0680032339.01.T05;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|56
Chr6 exon 38822254 38822424 38822254 ID=MsG0680032339.01.T05:exon:21152;Parent=MsG0680032339.01.T05
Chr6 CDS 38822254 38822424 38822254 ID=MsG0680032339.01.T05:cds;Parent=MsG0680032339.01.T05
Gene Sequence

>MsG0680032339.01.T01

ATGCTATTAGGGAATCTTATTGCTATTACCCAAACAAGCATGAAACGTATGCTTGCTTATTCGTCCATAGGTCAAATTGGATATGTAGTTATTGGAATAATTGTTGGAGACTCAAATGGTGGATATGCAAGCATGATAACTTATATGTTGTTCTATATCTCCATGAATCTAGGAACTTTTGCTTGCATTGTGTTATTTGGTCTACGTACTGGAACTGATAACATTCGAGATTATGCAGGATTATACACAAAAGATCCTTTTTTGGCTCTCTCTTTAGCCCTATGTCTATTATCCTTAGGAGGTCTTCCTCCACTAGCAGGTTTTTTCGGAAAACTTCATTTATTCTGGTGTGGATGGCAAGCAGGCCTATATTTCTTGGTTTCAATAGGACTTATTACAAGCGTTGTTTCTATCTACTATTATCCAAAAATAATTAAGTTATTAATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

>MsG0680032339.01.T04

ATGAATCTAGGAACTTTTGCTTGCATTGTGTTATTTGGTCTACGTACTGGAACTGATAACATTCGAGATTATGCAGGATTATACACAAAAGATCCTTTTTTGGCTCTCTCTTTAGCCCTATGTCTATTATCCTTAGGAGGTCTTCCTCCACTAGCAGGTTTTTTCGGAAAACTTCATTTATTCTGGTGTGGATGGCAAGCAGGCCTATATTTCTTGGTTTCAATAGGACTTATTACAAGCGTTGTTTCTATCTACTATTATCCAAAAATAATTAAGTTATTAATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

>MsG0680032339.01.T03

ATGATAACTTATATGTTGTTCTATATCTCCATGAATCTAGGAACTTTTGCTTGCATTGTGTTATTTGGTCTACGTACTGGAACTGATAACATTCGAGATTATGCAGGATTATACACAAAAGATCCTTTTTTGGCTCTCTCTTTAGCCCTATGTCTATTATCCTTAGGAGGTCTTCCTCCACTAGCAGGTTTTTTCGGAAAACTTCATTTATTCTGGTGTGGATGGCAAGCAGGCCTATATTTCTTGGTTTCAATAGGACTTATTACAAGCGTTGTTTCTATCTACTATTATCCAAAAATAATTAAGTTATTAATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

>MsG0680032339.01.T02

ATGAAACGTATGCTTGCTTATTCGTCCATAGGTCAAATTGGATATGTAGTTATTGGAATAATTGTTGGAGACTCAAATGGTGGATATGCAAGCATGATAACTTATATGTTGTTCTATATCTCCATGAATCTAGGAACTTTTGCTTGCATTGTGTTATTTGGTCTACGTACTGGAACTGATAACATTCGAGATTATGCAGGATTATACACAAAAGATCCTTTTTTGGCTCTCTCTTTAGCCCTATGTCTATTATCCTTAGGAGGTCTTCCTCCACTAGCAGGTTTTTTCGGAAAACTTCATTTATTCTGGTGTGGATGGCAAGCAGGCCTATATTTCTTGGTTTCAATAGGACTTATTACAAGCGTTGTTTCTATCTACTATTATCCAAAAATAATTAAGTTATTAATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

>MsG0680032339.01.T05

ATGACTGGACGAAACCAAGAAATAACTCCTCACGTGCGAAATTATAGAAGGTCTCCTGTAAGATCAAACAATTCCATCGAATTGAGTATGATTATATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCCATTATTGAAATTGCTCAGGATACCCTTTTTTAG

Protein sequence

>MsG0680032339.01.T01

MLLGNLIAITQTSMKRMLAYSSIGQIGYVVIGIIVGDSNGGYASMITYMLFYISMNLGTFACIVLFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWCGWQAGLYFLVSIGLITSVVSIYYYPKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*

>MsG0680032339.01.T04

MNLGTFACIVLFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWCGWQAGLYFLVSIGLITSVVSIYYYPKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*

>MsG0680032339.01.T03

MITYMLFYISMNLGTFACIVLFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWCGWQAGLYFLVSIGLITSVVSIYYYPKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*

>MsG0680032339.01.T02

MKRMLAYSSIGQIGYVVIGIIVGDSNGGYASMITYMLFYISMNLGTFACIVLFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWCGWQAGLYFLVSIGLITSVVSIYYYPKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*

>MsG0680032339.01.T05

MTGRNQEITPHVRNYRRSPVRSNNSIELSMIICVIASTIPGISMNPIIEIAQDTLF*