AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013980.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013980.01.T01 MTR_4g051097 96.364 55 2 0 1 55 1 55 1.03e-30 102
MsG0380013980.01.T01 MTR_0002s0540 95.745 47 2 0 1 47 1 47 9.91e-25 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013980.01.T01 ATCG00140 90.909 55 5 0 1 55 1 55 1.44e-28 97.4

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAAGCTTTAACTATTTA+TGG 0.037973 3:+48557336 MsG0380013980.01.T02:CDS
CGGAAGCAGAGGACAAAATA+AGG 0.270738 3:+48557287 MsG0380013980.01.T01:CDS
GCCGTCGGGCTTGCTTCTAT+TGG 0.311843 3:+48557205 MsG0380013980.01.T01:CDS
GCTGCTTCTGTTATCGCTGC+TGG 0.315135 3:+48557178 MsG0380013980.01.T01:CDS
TGGAAGCTTTAACTATTTAT+GGG 0.316470 3:+48557337 MsG0380013980.01.T02:CDS
GCTGCAGGGCAAGCTGTAGA+AGG 0.363265 3:+48557250 MsG0380013980.01.T01:CDS
GCTTCTATTGGACCTGGAGT+TGG 0.416315 3:+48557217 MsG0380013980.01.T01:CDS
TCGCTGCTGGGTTGGCCGTC+GGG 0.427770 3:+48557191 MsG0380013980.01.T01:CDS
ATCGCTGCTGGGTTGGCCGT+CGG 0.428270 3:+48557190 MsG0380013980.01.T01:CDS
AAAAGATTAAACAAAAGGAT+TGG 0.459809 3:-48557388 None:intergenic
AGCTTTAACTATTTATGGGC+TGG 0.460263 3:+48557341 MsG0380013980.01.T02:CDS
CTGCTTCTGTTATCGCTGCT+GGG 0.461552 3:+48557179 MsG0380013980.01.T01:CDS
GGAAGCAGAGGACAAAATAA+GGG 0.473459 3:+48557288 MsG0380013980.01.T01:CDS
AGAAGGGATTGCGCGACAAC+CGG 0.475077 3:+48557267 MsG0380013980.01.T01:CDS
GGGCTTGCTTCTATTGGACC+TGG 0.481695 3:+48557211 MsG0380013980.01.T01:CDS
GTTGGTCAAGGTACAGCTGC+AGG 0.497139 3:+48557235 MsG0380013980.01.T01:CDS
TTCTGTTATCGCTGCTGGGT+TGG 0.514112 3:+48557183 MsG0380013980.01.T01:CDS
ATTGGACCTGGAGTTGGTCA+AGG 0.543278 3:+48557223 MsG0380013980.01.T01:CDS
TGCGCGACAACCGGAAGCAG+AGG 0.554171 3:+48557276 MsG0380013980.01.T01:CDS
CTGCAGGGCAAGCTGTAGAA+GGG 0.582070 3:+48557251 MsG0380013980.01.T01:CDS
GCTGTACCTTGACCAACTCC+AGG 0.593501 3:-48557229 None:intergenic
TCCAATAGAAGCAAGCCCGA+CGG 0.638086 3:-48557206 None:intergenic
ACAGAAGCAGCAGAAATCAG+TGG 0.640161 3:-48557166 None:intergenic
TTGGTCAAGGTACAGCTGCA+GGG 0.643472 3:+48557236 MsG0380013980.01.T01:CDS
GAAGCAGAGGACAAAATAAG+GGG 0.658398 3:+48557289 MsG0380013980.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGGAAGCTTTAACTATTTA+TGG + Chr3:48557336-48557355 MsG0380013980.01.T02:CDS 25.0%
!! TGGAAGCTTTAACTATTTAT+GGG + Chr3:48557337-48557356 MsG0380013980.01.T02:CDS 25.0%
!!! TTTACTTAGTCTGGCTTTTA+TGG + Chr3:48557317-48557336 MsG0380013980.01.T01:CDS 30.0%
! AGCTTTAACTATTTATGGGC+TGG + Chr3:48557341-48557360 MsG0380013980.01.T02:CDS 35.0%
GAAGCAGAGGACAAAATAAG+GGG + Chr3:48557289-48557308 MsG0380013980.01.T01:CDS 40.0%
GGAAGCAGAGGACAAAATAA+GGG + Chr3:48557288-48557307 MsG0380013980.01.T01:CDS 40.0%
!! GGGGTACTTTTTACTTAGTC+TGG + Chr3:48557308-48557327 MsG0380013980.01.T01:CDS 40.0%
!!! CTTATTTTGTCCTCTGCTTC+CGG - Chr3:48557289-48557308 None:intergenic 40.0%
ACAGAAGCAGCAGAAATCAG+TGG - Chr3:48557169-48557188 None:intergenic 45.0%
CGGAAGCAGAGGACAAAATA+AGG + Chr3:48557287-48557306 MsG0380013980.01.T01:CDS 45.0%
CTGCTTCTGTTATCGCTGCT+GGG + Chr3:48557179-48557198 MsG0380013980.01.T01:CDS 50.0%
GCTTCTATTGGACCTGGAGT+TGG + Chr3:48557217-48557236 MsG0380013980.01.T01:CDS 50.0%
TCCAATAGAAGCAAGCCCGA+CGG - Chr3:48557209-48557228 None:intergenic 50.0%
TTCTGTTATCGCTGCTGGGT+TGG + Chr3:48557183-48557202 MsG0380013980.01.T01:CDS 50.0%
TTGGTCAAGGTACAGCTGCA+GGG + Chr3:48557236-48557255 MsG0380013980.01.T01:CDS 50.0%
! ATTGGACCTGGAGTTGGTCA+AGG + Chr3:48557223-48557242 MsG0380013980.01.T01:CDS 50.0%
AGAAGGGATTGCGCGACAAC+CGG + Chr3:48557267-48557286 MsG0380013980.01.T01:CDS 55.0%
CTGCAGGGCAAGCTGTAGAA+GGG + Chr3:48557251-48557270 MsG0380013980.01.T01:CDS 55.0%
GCTGCTTCTGTTATCGCTGC+TGG + Chr3:48557178-48557197 MsG0380013980.01.T01:CDS 55.0%
GCTGTACCTTGACCAACTCC+AGG - Chr3:48557232-48557251 None:intergenic 55.0%
GTTGGTCAAGGTACAGCTGC+AGG + Chr3:48557235-48557254 MsG0380013980.01.T01:CDS 55.0%
! GGGCTTGCTTCTATTGGACC+TGG + Chr3:48557211-48557230 MsG0380013980.01.T01:CDS 55.0%
GCTGCAGGGCAAGCTGTAGA+AGG + Chr3:48557250-48557269 MsG0380013980.01.T01:CDS 60.0%
! GCCGTCGGGCTTGCTTCTAT+TGG + Chr3:48557205-48557224 MsG0380013980.01.T01:CDS 60.0%
TGCGCGACAACCGGAAGCAG+AGG + Chr3:48557276-48557295 MsG0380013980.01.T01:CDS 65.0%
!! ATCGCTGCTGGGTTGGCCGT+CGG + Chr3:48557190-48557209 MsG0380013980.01.T01:CDS 65.0%
!! TCGCTGCTGGGTTGGCCGTC+GGG + Chr3:48557191-48557210 MsG0380013980.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48557160 48557404 48557160 ID=MsG0380013980.01;Name=MsG0380013980.01
Chr3 mRNA 48557160 48557348 48557160 ID=MsG0380013980.01.T01;Parent=MsG0380013980.01;Name=MsG0380013980.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|62
Chr3 exon 48557160 48557348 48557160 ID=MsG0380013980.01.T01:exon:8586;Parent=MsG0380013980.01.T01
Chr3 CDS 48557160 48557348 48557160 ID=MsG0380013980.01.T01:cds;Parent=MsG0380013980.01.T01
Chr3 mRNA 48557336 48557404 48557336 ID=MsG0380013980.01.T02;Parent=MsG0380013980.01;Name=MsG0380013980.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|22
Chr3 exon 48557336 48557404 48557336 ID=MsG0380013980.01.T02:exon:8587;Parent=MsG0380013980.01.T02
Chr3 CDS 48557336 48557404 48557336 ID=MsG0380013980.01.T02:cds;Parent=MsG0380013980.01.T02
Gene Sequence

>MsG0380013980.01.T02

ATGGAAGCTTTAACTATTTATGGGCTGGTTGTAGCATTAGCACTTTTATTTGCCAATCCTTTTGTTTAA

>MsG0380013980.01.T01

ATGGATCCACTGATTTCTGCTGCTTCTGTTATCGCTGCTGGGTTGGCCGTCGGGCTTGCTTCTATTGGACCTGGAGTTGGTCAAGGTACAGCTGCAGGGCAAGCTGTAGAAGGGATTGCGCGACAACCGGAAGCAGAGGACAAAATAAGGGGTACTTTTTACTTAGTCTGGCTTTTATGGAAGCTTTAA

Protein sequence

>MsG0380013980.01.T02

MEALTIYGLVVALALLFANPFV*

>MsG0380013980.01.T01

MDPLISAASVIAAGLAVGLASIGPGVGQGTAAGQAVEGIARQPEAEDKIRGTFYLVWLLWKL*