AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014633.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014633.01.T01 MTR_3g461540 65.417 240 30 4 3 199 2 231 4.33e-99 290
MsG0380014633.01.T01 MTR_8g445730 31.765 170 85 1 23 161 3 172 1.85e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014633.01.T01 AT5G56120 38.785 214 83 5 10 176 8 220 4.65e-36 129
MsG0380014633.01.T01 AT3G12870 31.056 161 76 3 36 161 11 171 8.08e-19 82.8

Find 52 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCGAGTGTTGAGATTAAA+TGG 0.237923 3:+57561106 MsG0380014633.01.T01:CDS
TGATGATGTTCTTGGTCTAC+TGG 0.277729 3:-57560570 None:intergenic
TTCCACCACCACCATTCATT+TGG 0.309071 3:-57560860 None:intergenic
AGGTGTTGGGAGGTGTATTC+AGG 0.310456 3:+57561015 MsG0380014633.01.T01:CDS
GATTCGGAGATTTCGGTTAA+AGG 0.313626 3:-57560657 None:intergenic
CCCGGGTGATTCGGAGATTT+CGG 0.344839 3:-57560664 None:intergenic
ACCTCTTACTCTTAAAGCTT+TGG 0.355477 3:+57560688 MsG0380014633.01.T01:CDS
GCCAAAGCTTTAAGAGTAAG+AGG 0.390218 3:-57560689 None:intergenic
TCCACCACCACCATTCATTT+GGG 0.390629 3:-57560859 None:intergenic
TCTAAAGAGTTGCAAGTGAA+TGG 0.391555 3:+57561070 MsG0380014633.01.T01:CDS
CCAACATTGCTTCATTCCTT+TGG 0.395413 3:-57563851 None:intergenic
ATGAAGTGAAAAGGAGTGTT+AGG 0.395626 3:-57560827 None:intergenic
AGGAATGATAGTAGAGGTGT+TGG 0.398719 3:+57561001 MsG0380014633.01.T01:CDS
GTTGATAGATCAATGGACTT+TGG 0.400152 3:+57563987 MsG0380014633.01.T01:CDS
TAAGAGGTGCCCGGGTGATT+CGG 0.414431 3:-57560673 None:intergenic
TCATTCTTCTTCATCTTCCA+TGG 0.415857 3:+57560798 MsG0380014633.01.T01:CDS
TTAAATGGACAGTGGTTACT+TGG 0.431450 3:+57561121 MsG0380014633.01.T01:CDS
TTCAGGAATTGAGGATGAAA+GGG 0.438603 3:+57561032 MsG0380014633.01.T01:CDS
ATTCGGAGATTTCGGTTAAA+GGG 0.444126 3:-57560656 None:intergenic
TTACCGCACTTGGCTCGGCT+TGG 0.444879 3:+57563884 MsG0380014633.01.T01:CDS
TCCGATTGATTTACCGCACT+TGG 0.444951 3:+57563874 MsG0380014633.01.T01:CDS
TTCAAGAAAAGAAACAAGAA+TGG 0.448093 3:+57560743 MsG0380014633.01.T01:CDS
GCTTCCAAGTTTGTTCTATG+TGG 0.453603 3:+57563685 MsG0380014633.01.T01:CDS
CAAACCACATAGAACAAACT+TGG 0.460260 3:-57563689 None:intergenic
CTAAAGAGTTGCAAGTGAAT+GGG 0.497169 3:+57561071 MsG0380014633.01.T01:CDS
GATGATGTTCTTGGTCTACT+GGG 0.500369 3:-57560569 None:intergenic
GGAGGTGTATTCAGGAATTG+AGG 0.501941 3:+57561023 MsG0380014633.01.T01:CDS
TTGATTTACCGCACTTGGCT+CGG 0.510376 3:+57563879 MsG0380014633.01.T01:CDS
CCAAAGGAATGAAGCAATGT+TGG 0.512491 3:+57563851 MsG0380014633.01.T01:CDS
GCCAAGTGCGGTAAATCAAT+CGG 0.512992 3:-57563875 None:intergenic
CGGAATTGTTGATAGATCAA+TGG 0.530778 3:+57563980 MsG0380014633.01.T01:CDS
CTTTAAGAGTAAGAGGTGCC+CGG 0.533485 3:-57560682 None:intergenic
CTAGCTAGTGATGGCGAAGA+CGG 0.536687 3:+57563960 MsG0380014633.01.T01:CDS
GGAATGATAGTAGAGGTGTT+GGG 0.538678 3:+57561002 MsG0380014633.01.T01:CDS
ATTCAGGAATTGAGGATGAA+AGG 0.541806 3:+57561031 MsG0380014633.01.T01:CDS
GATCAATGGACTTTGGATCA+TGG 0.547782 3:+57563994 MsG0380014633.01.T01:CDS
ACCCAAATGAATGGTGGTGG+TGG 0.550058 3:+57560858 MsG0380014633.01.T01:CDS
ACCGAAATCTCCGAATCACC+CGG 0.553450 3:+57560663 MsG0380014633.01.T01:CDS
GCTTCATTCCTTTGGCAGAG+AGG 0.555837 3:-57563843 None:intergenic
CATAGAACAAACTTGGAAGC+AGG 0.560691 3:-57563682 None:intergenic
GATCTTGATCTAGCTAGTGA+TGG 0.569597 3:+57563951 MsG0380014633.01.T01:CDS
TTTAAGAGTAAGAGGTGCCC+GGG 0.579280 3:-57560681 None:intergenic
CGGAGATTTCGGTTAAAGGG+TGG 0.601783 3:-57560653 None:intergenic
AAGGAGAGGAATGATAGTAG+AGG 0.610302 3:+57560995 MsG0380014633.01.T01:CDS
AAAACCCAAATGAATGGTGG+TGG 0.629504 3:+57560855 MsG0380014633.01.T01:CDS
ATGATAGTAGAGGTGTTGGG+AGG 0.635080 3:+57561005 MsG0380014633.01.T01:CDS
CCGAAATCTCCGAATCACCC+GGG 0.650790 3:+57560664 MsG0380014633.01.T01:CDS
TGGGAACAGTTACAAAGGAG+AGG 0.654507 3:+57560981 MsG0380014633.01.T01:intron
CAACCAAGCCGAGCCAAGTG+CGG 0.658335 3:-57563887 None:intergenic
TCAGGAATTGAGGATGAAAG+GGG 0.667293 3:+57561033 MsG0380014633.01.T01:CDS
AAGTGTGGGAACAGTTACAA+AGG 0.672817 3:+57560976 MsG0380014633.01.T01:intron
TGTTGAGATTAAATGGACAG+TGG 0.675519 3:+57561113 MsG0380014633.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTAAAAAATA+TGG - Chr3:57562741-57562760 None:intergenic 0.0%
!!! TTTTTTATATTTTTGCTTTT+TGG - Chr3:57562588-57562607 None:intergenic 10.0%
!! AGCAAAAATATAAAAAATAG+AGG + Chr3:57562591-57562610 MsG0380014633.01.T01:intron 15.0%
!! ATTAATTTATTATACTACGA+TGG + Chr3:57563002-57563021 MsG0380014633.01.T01:intron 15.0%
!!! AAATAAGTTGTTTTGAAAAA+AGG - Chr3:57562347-57562366 None:intergenic 15.0%
!! AATGGTTCGAATAAATAAAA+CGG - Chr3:57562232-57562251 None:intergenic 20.0%
!! ATATATATAGCCAATACAAA+AGG - Chr3:57561611-57561630 None:intergenic 20.0%
!! TTTGTTTATTGTTAATGATG+AGG + Chr3:57561254-57561273 MsG0380014633.01.T01:intron 20.0%
!!! ATAAACCAGTTTTTTTAAAG+TGG - Chr3:57562150-57562169 None:intergenic 20.0%
!!! ATTGTTTTTAGATTTAACGT+TGG + Chr3:57562500-57562519 MsG0380014633.01.T01:intron 20.0%
!!! CTTTCTTAATTTGTGTTATT+TGG + Chr3:57561292-57561311 MsG0380014633.01.T01:intron 20.0%
!!! GAGATTTGTTTAGTTTTTTA+TGG + Chr3:57561321-57561340 MsG0380014633.01.T01:intron 20.0%
!!! GATTTTAGTCATTGTTTATT+AGG + Chr3:57562317-57562336 MsG0380014633.01.T01:intron 20.0%
!!! TAAAAAACTGATTTCTTTCT+CGG + Chr3:57562370-57562389 MsG0380014633.01.T01:intron 20.0%
!!! TGAGATTTGTTTAGTTTTTA+TGG + Chr3:57563801-57563820 MsG0380014633.01.T01:intron 20.0%
!!! TTAAACCACTTTAAAAAAAC+TGG + Chr3:57562142-57562161 MsG0380014633.01.T01:intron 20.0%
! AAAACCAGTTCAAGAATAAT+TGG - Chr3:57562288-57562307 None:intergenic 25.0%
! AAGTAACAACTTTAGCTAAT+AGG + Chr3:57562028-57562047 MsG0380014633.01.T01:intron 25.0%
! ACCACAAAAAAAAAACAACT+TGG - Chr3:57562618-57562637 None:intergenic 25.0%
! ATAAACAATGACTAAAATCC+CGG - Chr3:57562316-57562335 None:intergenic 25.0%
! CATATATCTGTGAATTCTAA+CGG - Chr3:57562837-57562856 None:intergenic 25.0%
! TTCAAGAAAAGAAACAAGAA+TGG + Chr3:57560743-57560762 MsG0380014633.01.T01:CDS 25.0%
!! AAGAATAATTGGTTCAAAAC+TGG - Chr3:57562277-57562296 None:intergenic 25.0%
!! ATCAAGATTATGATGATGTT+TGG + Chr3:57561230-57561249 MsG0380014633.01.T01:intron 25.0%
!! TTTCTTTTCTTGAAATGCTT+TGG - Chr3:57563227-57563246 None:intergenic 25.0%
!!! ACCAAGTTGTTTTTTTTTTG+TGG + Chr3:57562614-57562633 MsG0380014633.01.T01:intron 25.0%
!!! AGTTGTTTTTTTTTTGTGGT+CGG + Chr3:57562618-57562637 MsG0380014633.01.T01:intron 25.0%
!!! CAATTGATTTTAGTGAATCA+TGG + Chr3:57561968-57561987 MsG0380014633.01.T01:intron 25.0%
!!! GTCTTCTTAATTTGTGTATT+TGG + Chr3:57563773-57563792 MsG0380014633.01.T01:intron 25.0%
AAAACGGTTCAAAACGTAAA+CGG - Chr3:57562216-57562235 None:intergenic 30.0%
AACAACTTTAGCTAATAGGA+TGG + Chr3:57562032-57562051 MsG0380014633.01.T01:intron 30.0%
AACGAATTGATCCATTAATG+TGG - Chr3:57562120-57562139 None:intergenic 30.0%
AAGTGAAATTGGTTAGGTAT+TGG + Chr3:57560952-57560971 MsG0380014633.01.T01:intron 30.0%
ACAATTCAATTTGTGAGAGA+TGG + Chr3:57561719-57561738 MsG0380014633.01.T01:intron 30.0%
ATCATATAGTGATAGCAAGT+CGG + Chr3:57561787-57561806 MsG0380014633.01.T01:intron 30.0%
ATTACAAGATCATCCATCAA+AGG - Chr3:57561761-57561780 None:intergenic 30.0%
ATTCAGGAATTGAGATGAAA+GGG + Chr3:57563517-57563536 MsG0380014633.01.T01:intron 30.0%
GCATGAATGAAAGAAAATGA+AGG - Chr3:57561874-57561893 None:intergenic 30.0%
GTAGATTTAAGAGAGTTAAG+TGG + Chr3:57561643-57561662 MsG0380014633.01.T01:intron 30.0%
GTATGAATAATGTAAGGTCT+GGG - Chr3:57562653-57562672 None:intergenic 30.0%
GTTGAAAGTGAAATCAATGA+TGG - Chr3:57560906-57560925 None:intergenic 30.0%
TATGAATAATGTAAGGTCTG+GGG - Chr3:57562652-57562671 None:intergenic 30.0%
TATTCAGGAATTGAGATGAA+AGG + Chr3:57563516-57563535 MsG0380014633.01.T01:intron 30.0%
TCACATAAGTGCAGTTTAAA+AGG + Chr3:57562470-57562489 MsG0380014633.01.T01:intron 30.0%
TCATGGACAAGTACAAAAAA+TGG + Chr3:57561985-57562004 MsG0380014633.01.T01:intron 30.0%
TGAACCAATTATTCTTGAAC+TGG + Chr3:57562281-57562300 MsG0380014633.01.T01:intron 30.0%
TGATAGATTCAATCCTTTGA+TGG + Chr3:57561745-57561764 MsG0380014633.01.T01:intron 30.0%
TGTTAACGTTATTGTTGTGT+TGG - Chr3:57562878-57562897 None:intergenic 30.0%
TTGATTTAGATCATGGACTT+TGG + Chr3:57561507-57561526 MsG0380014633.01.T01:intron 30.0%
! AACTGGTTTAAAACGTAAAC+CGG - Chr3:57562260-57562279 None:intergenic 30.0%
! AATGAAGTGAGTTGAAAAAG+TGG - Chr3:57560783-57560802 None:intergenic 30.0%
! ACATGATATGCTTGTTGATT+AGG - Chr3:57561559-57561578 None:intergenic 30.0%
! CAGTTGGTATGAATAATGTA+AGG - Chr3:57562659-57562678 None:intergenic 30.0%
! GCTTGTTGATTAGGTAAAAA+TGG - Chr3:57561550-57561569 None:intergenic 30.0%
! TGAAGTGAGTTGAAAAAGTT+CGG - Chr3:57560781-57560800 None:intergenic 30.0%
! TTCACTTGTGTTTGTGTTTT+TGG + Chr3:57560926-57560945 MsG0380014633.01.T01:intron 30.0%
! TTCTTTTCTTGAAATGCGTT+TGG - Chr3:57560736-57560755 None:intergenic 30.0%
!! GGAATTGTTGATTTAGATCA+TGG + Chr3:57561500-57561519 MsG0380014633.01.T01:intron 30.0%
!!! GTTGTTTTTTTTTTGTGGTC+GGG + Chr3:57562619-57562638 MsG0380014633.01.T01:intron 30.0%
!!! GTTTGTCTTGTTTGTTTTAC+AGG + Chr3:57561165-57561184 MsG0380014633.01.T01:CDS 30.0%
!!! GTTTTTGGTTCAAGTGAAAT+TGG + Chr3:57560941-57560960 MsG0380014633.01.T01:intron 30.0%
!!! TCTTGTTTGTTTTACAGGTT+TGG + Chr3:57561170-57561189 MsG0380014633.01.T01:intron 30.0%
!!! TGTTGTTTTGATGATGTTCT+TGG - Chr3:57560581-57560600 None:intergenic 30.0%
!!! TGTTTTGGTTCAAGTGAAAT+TGG + Chr3:57563426-57563445 MsG0380014633.01.T01:intron 30.0%
!!! TTTTTTGCCATGAAGTGAAA+AGG - Chr3:57560839-57560858 None:intergenic 30.0%
AAAAAACCCAAAGAATGGTG+TGG + Chr3:57563341-57563360 MsG0380014633.01.T01:intron 35.0%
ACCTCTTACTCTTAAAGCTT+TGG + Chr3:57560688-57560707 MsG0380014633.01.T01:CDS 35.0%
AGAGTAAAACTCCTACATCT+GGG - Chr3:57561822-57561841 None:intergenic 35.0%
AGCATAATATCCTGTCATTG+TGG + Chr3:57562061-57562080 MsG0380014633.01.T01:intron 35.0%
ATGGCAAAAAACCCAAAGAA+TGG + Chr3:57563336-57563355 MsG0380014633.01.T01:intron 35.0%
ATTAAATGGACAGTGGTTAC+TGG + Chr3:57561120-57561139 MsG0380014633.01.T01:CDS 35.0%
ATTCAGGAATTGAGGATGAA+AGG + Chr3:57561031-57561050 MsG0380014633.01.T01:CDS 35.0%
ATTCGGAGATTTCGGTTAAA+GGG - Chr3:57560659-57560678 None:intergenic 35.0%
CAAAAAACCCAAATGAATGG+TGG + Chr3:57560852-57560871 MsG0380014633.01.T01:CDS 35.0%
CAAACCACATAGAACAAACT+TGG - Chr3:57561208-57561227 None:intergenic 35.0%
CATAATATCCTGTCATTGTG+GGG + Chr3:57562063-57562082 MsG0380014633.01.T01:intron 35.0%
CATTTCTTCTTCATCTTCCA+TGG + Chr3:57563286-57563305 MsG0380014633.01.T01:intron 35.0%
CTAAAGAGTTGCAAGTGAAT+GGG + Chr3:57561071-57561090 MsG0380014633.01.T01:CDS 35.0%
CTCTCTTAAATCTACTCACA+AGG - Chr3:57561638-57561657 None:intergenic 35.0%
GAAGGATGAACACAAGAATT+TGG - Chr3:57561856-57561875 None:intergenic 35.0%
GCATAATATCCTGTCATTGT+GGG + Chr3:57562062-57562081 MsG0380014633.01.T01:intron 35.0%
GGTATGAATAATGTAAGGTC+TGG - Chr3:57562654-57562673 None:intergenic 35.0%
GTACAAAAAATGGCCTCAAA+AGG + Chr3:57561995-57562014 MsG0380014633.01.T01:intron 35.0%
TAGAATTCAGTGCACATATC+AGG + Chr3:57561691-57561710 MsG0380014633.01.T01:intron 35.0%
TCATGTGTTCATCATTGATC+TGG + Chr3:57561931-57561950 MsG0380014633.01.T01:intron 35.0%
TCATTCTTCTTCATCTTCCA+TGG + Chr3:57560798-57560817 MsG0380014633.01.T01:CDS 35.0%
TCTAAAGAGTTGCAAGTGAA+TGG + Chr3:57561070-57561089 MsG0380014633.01.T01:CDS 35.0%
TGGCAAAAAACCCAAATGAA+TGG + Chr3:57560849-57560868 MsG0380014633.01.T01:CDS 35.0%
TGGTTCAAGTGAAATTGGTT+AGG + Chr3:57560946-57560965 MsG0380014633.01.T01:intron 35.0%
TGTTGAGATTAAATGGACAG+TGG + Chr3:57561113-57561132 MsG0380014633.01.T01:CDS 35.0%
TTAAATGGACAGTGGTTACT+TGG + Chr3:57561121-57561140 MsG0380014633.01.T01:CDS 35.0%
TTATTCGAACCATTTCGAAC+CGG + Chr3:57562238-57562257 MsG0380014633.01.T01:intron 35.0%
TTCAGGAATTGAGATGAAAG+GGG + Chr3:57563518-57563537 MsG0380014633.01.T01:intron 35.0%
TTCAGGAATTGAGGATGAAA+GGG + Chr3:57561032-57561051 MsG0380014633.01.T01:CDS 35.0%
TTGGAACTGAACCACATTAA+TGG + Chr3:57562106-57562125 MsG0380014633.01.T01:intron 35.0%
! CGGAATTGTTGATAGATCAA+TGG + Chr3:57563980-57563999 MsG0380014633.01.T01:CDS 35.0%
! CTTCACTTGTGTTTGTGTTT+TGG + Chr3:57560925-57560944 MsG0380014633.01.T01:intron 35.0%
! CTTTGCATTGAAACCTTTTG+AGG - Chr3:57562011-57562030 None:intergenic 35.0%
! GTTGATAGATCAATGGACTT+TGG + Chr3:57563987-57564006 MsG0380014633.01.T01:CDS 35.0%
! TAACACTCCTTTTCACTTCA+TGG + Chr3:57560829-57560848 MsG0380014633.01.T01:CDS 35.0%
! TAGGAGTTTTACTCTGAACT+GGG + Chr3:57561827-57561846 MsG0380014633.01.T01:intron 35.0%
! TTTTTCTCAGATGTATCCGT+TGG + Chr3:57562764-57562783 MsG0380014633.01.T01:intron 35.0%
!! ATGAAGTGAAAAGGAGTGTT+AGG - Chr3:57560830-57560849 None:intergenic 35.0%
!! GTTCGAGTGTTGAGATTAAA+TGG + Chr3:57561106-57561125 MsG0380014633.01.T01:CDS 35.0%
!! TGTGTGTTTGATGATGTTCT+TGG - Chr3:57563073-57563092 None:intergenic 35.0%
!! TTGATGATGTTCTTGGTCTA+GGG - Chr3:57560574-57560593 None:intergenic 35.0%
!! TTTGATGATGTTCTTGGTCT+AGG - Chr3:57560575-57560594 None:intergenic 35.0%
!!! CTTGAACTGGTTTTCAAAAC+CGG + Chr3:57562294-57562313 MsG0380014633.01.T01:intron 35.0%
!!! GTAACTTGCTCCTTTTGTAT+TGG + Chr3:57561598-57561617 MsG0380014633.01.T01:intron 35.0%
!!! TTGAACTGGTTTTCAAAACC+GGG + Chr3:57562295-57562314 MsG0380014633.01.T01:intron 35.0%
AAAACCCAAATGAATGGTGG+TGG + Chr3:57560855-57560874 MsG0380014633.01.T01:CDS 40.0%
AAGGAGAGGAATGATAGTAG+AGG + Chr3:57560995-57561014 MsG0380014633.01.T01:CDS 40.0%
AAGTGTGGGAACAGTTACAA+AGG + Chr3:57560976-57560995 MsG0380014633.01.T01:intron 40.0%
AGATCATGGACTTTGGATCA+TGG + Chr3:57561514-57561533 MsG0380014633.01.T01:intron 40.0%
AGTAAAACTCCTACATCTGG+GGG - Chr3:57561820-57561839 None:intergenic 40.0%
AGTGTGGGAACAGTTACAAA+GGG + Chr3:57560977-57560996 MsG0380014633.01.T01:intron 40.0%
CAGAGTAAAACTCCTACATC+TGG - Chr3:57561823-57561842 None:intergenic 40.0%
CATAGAACAAACTTGGAAGC+AGG - Chr3:57561201-57561220 None:intergenic 40.0%
CCAAAGGAATGAAGCAATGT+TGG + Chr3:57561371-57561390 MsG0380014633.01.T01:intron 40.0%
CCAACATTGCTTCATTCCTT+TGG - Chr3:57561374-57561393 None:intergenic 40.0%
GAGTAAAACTCCTACATCTG+GGG - Chr3:57561821-57561840 None:intergenic 40.0%
GATCTTGATCTAGCTAGTGA+TGG + Chr3:57561469-57561488 MsG0380014633.01.T01:intron 40.0%
GATGATGTTCTTGGTCTACT+GGG - Chr3:57560572-57560591 None:intergenic 40.0%
GATTCGGAGATTTCGGTTAA+AGG - Chr3:57560660-57560679 None:intergenic 40.0%
GATTCGGAGATTTCGGTTAA+GGG - Chr3:57560660-57560679 None:intergenic 40.0%
GTGGAAGTTGTAAGATATGG+TGG + Chr3:57560877-57560896 MsG0380014633.01.T01:intron 40.0%
TATTTCACACCGTCTTCCAA+CGG - Chr3:57562783-57562802 None:intergenic 40.0%
TCAGGAATTGAGGATGAAAG+GGG + Chr3:57561033-57561052 MsG0380014633.01.T01:CDS 40.0%
TGTGTTCATCATTGATCTGG+TGG + Chr3:57561934-57561953 MsG0380014633.01.T01:intron 40.0%
TGTGTTGGAAGAGAAAGATG+TGG - Chr3:57562863-57562882 None:intergenic 40.0%
TTCAAAAGCCAGTTCCAGTT+CGG + Chr3:57562172-57562191 MsG0380014633.01.T01:intron 40.0%
! AGGAATGATAGTAGAGGTGT+TGG + Chr3:57561001-57561020 MsG0380014633.01.T01:CDS 40.0%
! GATCAATGGACTTTGGATCA+TGG + Chr3:57563994-57564013 MsG0380014633.01.T01:CDS 40.0%
! GCTTCCAAGTTTGTTCTATG+TGG + Chr3:57561201-57561220 MsG0380014633.01.T01:intron 40.0%
! GTAGGAGTTTTACTCTGAAC+TGG + Chr3:57561826-57561845 MsG0380014633.01.T01:intron 40.0%
! GTGGTGGAAGTTGTAAGATA+TGG + Chr3:57560874-57560893 MsG0380014633.01.T01:intron 40.0%
! TGATGATGTTCTTGGTCTAC+TGG - Chr3:57560573-57560592 None:intergenic 40.0%
! TGATTCGGAGATTTCGGTTA+AGG - Chr3:57560661-57560680 None:intergenic 40.0%
!! AAACGTAAACCGGTTCGAAA+TGG - Chr3:57562250-57562269 None:intergenic 40.0%
!! AAACGTAAACGGTTCTGAAC+CGG - Chr3:57562205-57562224 None:intergenic 40.0%
!! GCCAAAGCTTTAAGAGTAAG+AGG - Chr3:57560692-57560711 None:intergenic 40.0%
!! GGAATGATAGTAGAGGTGTT+GGG + Chr3:57561002-57561021 MsG0380014633.01.T01:CDS 40.0%
!! GGTTAGGTATTGGAAAGTGT+GGG + Chr3:57560962-57560981 MsG0380014633.01.T01:intron 40.0%
!! GTTCATGGTTCTAACTGCAT+TGG + Chr3:57562087-57562106 MsG0380014633.01.T01:intron 40.0%
!! TGGTTAGGTATTGGAAAGTG+TGG + Chr3:57560961-57560980 MsG0380014633.01.T01:intron 40.0%
AAACCCAAAGAATGGTGTGG+TGG + Chr3:57563344-57563363 MsG0380014633.01.T01:intron 45.0%
ACTTCCACCACACCATTCTT+TGG - Chr3:57563351-57563370 None:intergenic 45.0%
AGGGAGAGGAATGATAGTAG+AGG + Chr3:57563481-57563500 MsG0380014633.01.T01:intron 45.0%
CAGATGTATCCGTTGGAAGA+CGG + Chr3:57562771-57562790 MsG0380014633.01.T01:intron 45.0%
CTTCCACCACACCATTCTTT+GGG - Chr3:57563350-57563369 None:intergenic 45.0%
GCCAAGTGCGGTAAATCAAT+CGG - Chr3:57561398-57561417 None:intergenic 45.0%
GTGTTAAACCGAACTGGAAC+TGG - Chr3:57562183-57562202 None:intergenic 45.0%
GTTCCAGTTCGGTTTAACAC+CGG + Chr3:57562183-57562202 MsG0380014633.01.T01:intron 45.0%
TCATACCAACTGAGCTAACG+AGG + Chr3:57562667-57562686 MsG0380014633.01.T01:intron 45.0%
TCCACCACCACCATTCATTT+GGG - Chr3:57560862-57560881 None:intergenic 45.0%
TGGGAACAGTTACAAAGGAG+AGG + Chr3:57560981-57561000 MsG0380014633.01.T01:intron 45.0%
TTCAGTGCACATATCAGGAG+AGG + Chr3:57561696-57561715 MsG0380014633.01.T01:intron 45.0%
TTCCACCACCACCATTCATT+TGG - Chr3:57560863-57560882 None:intergenic 45.0%
TTGATTTACCGCACTTGGCT+CGG + Chr3:57561399-57561418 MsG0380014633.01.T01:intron 45.0%
! GGAGGTGTATTCAGGAATTG+AGG + Chr3:57561023-57561042 MsG0380014633.01.T01:CDS 45.0%
! GGAGTGTTAGGAAAAAGCCA+TGG - Chr3:57560818-57560837 None:intergenic 45.0%
! TCCGATTGATTTACCGCACT+TGG + Chr3:57561394-57561413 MsG0380014633.01.T01:intron 45.0%
!! ATGATAGTAGAGGTGTTGGG+AGG + Chr3:57561005-57561024 MsG0380014633.01.T01:CDS 45.0%
!! CTTTAAGAGTAAGAGGTGCC+CGG - Chr3:57560685-57560704 None:intergenic 45.0%
!! TTTAAGAGTAAGAGGTGCCC+GGG - Chr3:57560684-57560703 None:intergenic 45.0%
!! TTTTTCAGCCTCTCTGCCAA+AGG + Chr3:57561355-57561374 MsG0380014633.01.T01:intron 45.0%
ACCGAAATCTCCGAATCACC+CGG + Chr3:57560663-57560682 MsG0380014633.01.T01:CDS 50.0%
CATGAACACCCCACAATGAC+AGG - Chr3:57562074-57562093 None:intergenic 50.0%
CGGAGATTTCGGTTAAAGGG+TGG - Chr3:57560656-57560675 None:intergenic 50.0%
GCTTCATTCCTTTGGCAGAG+AGG - Chr3:57561366-57561385 None:intergenic 50.0%
GGGAACAGTTACAAAGGGAG+AGG + Chr3:57563467-57563486 MsG0380014633.01.T01:intron 50.0%
TCGGAGATTTCGGTTAAGGG+TGG - Chr3:57563148-57563167 None:intergenic 50.0%
! CTAGCTAGTGATGGCGAAGA+CGG + Chr3:57561478-57561497 MsG0380014633.01.T01:intron 50.0%
! CTGTCATTGTGGGGTGTTCA+TGG + Chr3:57562072-57562091 MsG0380014633.01.T01:intron 50.0%
! GAACCGGTGTTAAACCGAAC+TGG - Chr3:57562189-57562208 None:intergenic 50.0%
! TAGCTAGTGATGGCGAAGAC+GGG + Chr3:57561479-57561498 MsG0380014633.01.T01:intron 50.0%
!! ACCCAAATGAATGGTGGTGG+TGG + Chr3:57560858-57560877 MsG0380014633.01.T01:CDS 50.0%
!! AGGTGTTGGGAGGTGTATTC+AGG + Chr3:57561015-57561034 MsG0380014633.01.T01:CDS 50.0%
ACTGAGCTAACGAGGACAGG+AGG + Chr3:57562675-57562694 MsG0380014633.01.T01:intron 55.0%
CAACAATGCCGAGCCAAGTG+CGG - Chr3:57561410-57561429 None:intergenic 55.0%
CCAACTGAGCTAACGAGGAC+AGG + Chr3:57562672-57562691 MsG0380014633.01.T01:intron 55.0%
CCGAAATCTCCGAATCACCC+GGG + Chr3:57560664-57560683 MsG0380014633.01.T01:CDS 55.0%
CCTGTCCTCGTTAGCTCAGT+TGG - Chr3:57562675-57562694 None:intergenic 55.0%
GGATTCTGTCCCCCAGATGT+AGG + Chr3:57561808-57561827 MsG0380014633.01.T01:intron 55.0%
! CCCGGGTGATTCGGAGATTT+CGG - Chr3:57560667-57560686 None:intergenic 55.0%
!! TAAGAGGTGCCCGGGTGATT+CGG - Chr3:57560676-57560695 None:intergenic 55.0%
CAACCAAGCCGAGCCAAGTG+CGG - Chr3:57563890-57563909 None:intergenic 60.0%
!! TTACCGCACTTGGCTCGGCT+TGG + Chr3:57563884-57563903 MsG0380014633.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 57560528 57564040 57560528 ID=MsG0380014633.01;Name=MsG0380014633.01
Chr3 mRNA 57560528 57564040 57560528 ID=MsG0380014633.01.T01;Parent=MsG0380014633.01;Name=MsG0380014633.01.T01;_AED=0.40;_eAED=0.59;_QI=0|0|0|1|1|1|4|0|270
Chr3 exon 57560528 57560885 57560528 ID=MsG0380014633.01.T01:exon:10666;Parent=MsG0380014633.01.T01
Chr3 exon 57560990 57561186 57560990 ID=MsG0380014633.01.T01:exon:10667;Parent=MsG0380014633.01.T01
Chr3 exon 57563671 57563730 57563671 ID=MsG0380014633.01.T01:exon:10668;Parent=MsG0380014633.01.T01
Chr3 exon 57563843 57564040 57563843 ID=MsG0380014633.01.T01:exon:10669;Parent=MsG0380014633.01.T01
Chr3 CDS 57560528 57560885 57560528 ID=MsG0380014633.01.T01:cds;Parent=MsG0380014633.01.T01
Chr3 CDS 57560990 57561186 57560990 ID=MsG0380014633.01.T01:cds;Parent=MsG0380014633.01.T01
Chr3 CDS 57563671 57563730 57563671 ID=MsG0380014633.01.T01:cds;Parent=MsG0380014633.01.T01
Chr3 CDS 57563843 57564040 57563843 ID=MsG0380014633.01.T01:cds;Parent=MsG0380014633.01.T01
Gene Sequence

>MsG0380014633.01.T01

ATGGCTGCTGAAACAAACAACACTCATCACATTGTTGAAATCCCAGTAGACCAAGAACATCATCAAAACAACAACAACAATAACAAAGCTTTGTTATGTTCTAACATGTTCGATGCAATCGACGACCACCCTTTAACCGAAATCTCCGAATCACCCGGGCACCTCTTACTCTTAAAGCTTTGGCAAAGAGAAGAAAATCTCTTCGCCAAACGCATTTCAAGAAAAGAAACAAGAATGGATTCAATAAAATCCGAACTTTTTCAACTCACTTCATTCTTCTTCATCTTCCATGGCTTTTTCCTAACACTCCTTTTCACTTCATGGCAAAAAACCCAAATGAATGGTGGTGGTGGAAGTTTTACAAAGGAGAGGAATGATAGTAGAGGTGTTGGGAGGTGTATTCAGGAATTGAGGATGAAAGGGGCTAGTTTTGATTTGTCTAAAGAGTTGCAAGTGAATGGGAAAAAGATGAAAGTTCGAGTGTTGAGATTAAATGGACAGTGGTTACTTGGTTTAATCGTTATTTGCTTACTGTTTGTCTTGTTTGTTTTACAGGTTTGGCTTTTCCTGCTTCCAAGTTTGTTCTATGTGGTTTGTGATCAAGATTATGATGATCCTCTCTGCCAAAGGAATGAAGCAATGTTGGTCCGATTGATTTACCGCACTTGGCTCGGCTTGGTTGTTGAGAAGATTAGATCGTTGAGATCACGATATGAGTCATTTGATCTTGATCTAGCTAGTGATGGCGAAGACGGAATTGTTGATAGATCAATGGACTTTGGATCATGGCTCAGCTCGTCCATTTTTACCTAA
Protein sequence

>MsG0380014633.01.T01

MAAETNNTHHIVEIPVDQEHHQNNNNNNKALLCSNMFDAIDDHPLTEISESPGHLLLLKLWQREENLFAKRISRKETRMDSIKSELFQLTSFFFIFHGFFLTLLFTSWQKTQMNGGGGSFTKERNDSRGVGRCIQELRMKGASFDLSKELQVNGKKMKVRVLRLNGQWLLGLIVICLLFVLFVLQVWLFLLPSLFYVVCDQDYDDPLCQRNEAMLVRLIYRTWLGLVVEKIRSLRSRYESFDLDLASDGEDGIVDRSMDFGSWLSSSIFT*