AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025506.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025506.01.T01 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025506.01.T01 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.15e-95 275
MsG0580025506.01.T01 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0580025506.01.T01 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0580025506.01.T01 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0580025506.01.T01 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.44e-92 264
MsG0580025506.01.T01 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.38e-91 262
MsG0580025506.01.T01 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0580025506.01.T01 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0580025506.01.T01 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.82e-82 238
MsG0580025506.01.T01 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.64e-75 219
MsG0580025506.01.T01 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.05e-65 195
MsG0580025506.01.T01 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.74e-56 171
MsG0580025506.01.T01 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.96e-52 160
MsG0580025506.01.T01 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.69e-45 144
MsG0580025506.01.T01 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.67e-43 139
MsG0580025506.01.T01 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.74e-42 137
MsG0580025506.01.T01 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.50e-40 132
MsG0580025506.01.T01 MTR_8g027840 55.102 98 43 1 37 133 28 125 5.01e-30 105
MsG0580025506.01.T01 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.48e-25 92.4
MsG0580025506.01.T01 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.75e-20 80.9
MsG0580025506.01.T01 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.26e-18 73.6
MsG0580025506.01.T01 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.81e-15 66.2
MsG0580025506.01.T01 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.48e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025506.01.T01 AT5G65360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025506.01.T01 AT5G10390 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025506.01.T01 AT5G10400 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025506.01.T01 AT3G27360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025506.01.T01 AT1G09200 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025506.01.T01 AT5G10980 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025506.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025506.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025506.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025506.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025506.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025506.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025506.01.T01 AT4G40030 97.059 136 4 0 1 136 29 164 7.04e-93 266
MsG0580025506.01.T01 AT5G65350 94.853 136 7 0 1 136 1 136 5.30e-91 260
MsG0580025506.01.T01 AT1G75600 93.382 136 9 0 1 136 1 136 1.25e-89 256
MsG0580025506.01.T01 AT1G13370 93.382 136 9 0 1 136 1 136 5.55e-89 254
MsG0580025506.01.T01 AT1G19890 89.781 137 13 1 1 136 1 137 1.07e-83 241
MsG0580025506.01.T01 AT5G12910 71.111 135 34 2 1 135 1 130 9.89e-67 198
MsG0580025506.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0580025506.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0580025506.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0580025506.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 36 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTTGCTGGAGCAGATTTC+CGG 0.208927 5:+19716830 None:intergenic
TTCTTGACTCCTCCGGTTGC+TGG 0.267204 5:+19716817 None:intergenic
TTGGTGGCTAGCTGCTTTCT+TGG 0.277587 5:+19716859 None:intergenic
TCACGAAGTGCGACAGTTCC+AGG 0.307100 5:+19716778 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 5:-19716622 MsG0580025506.01.T01:CDS
GAGCGGAAACAGCGCTGCTT+TGG 0.363298 5:+19716653 None:intergenic
GGTTGCTGGAGCAGATTTCC+GGG 0.375897 5:+19716831 None:intergenic
CAGTGCTCTTCTGATACTTC+CGG 0.409692 5:+19716752 None:intergenic
AGCAGATTTCCGGGCAGCTT+TGG 0.417274 5:+19716840 None:intergenic
CTTTCTTGGTGCCTTTCCTC+CGG 0.425692 5:+19716873 None:intergenic
CGGAAATCTGCTCCAGCAAC+CGG 0.425838 5:-19716829 MsG0580025506.01.T01:CDS
TTATCTTGTTGGTTTGTTTG+AGG 0.428809 5:-19716611 MsG0580025506.01.T01:CDS
CTGTCGCACTTCGTGAGATC+CGG 0.429742 5:-19716771 MsG0580025506.01.T01:CDS
CGATTTCACGAACAAGTCTC+TGG 0.432729 5:+19716704 None:intergenic
AGATTTCCGGGCAGCTTTGG+TGG 0.468887 5:+19716843 None:intergenic
GTGTGGTTTCTTGACTCCTC+CGG 0.472650 5:+19716810 None:intergenic
AGCTAGCCACCAAAGCTGCC+CGG 0.491500 5:-19716849 MsG0580025506.01.T01:CDS
CGAGCGAGTTGAATATCCTT+AGG 0.499677 5:+19716544 None:intergenic
AAACAGCGCTGCTTTGGAAT+CGG 0.500325 5:+19716659 None:intergenic
AGTAACTCTCTTAGCATGAA+TGG 0.521941 5:+19716573 None:intergenic
TCGCAAATCCACCGGAGGAA+AGG 0.532804 5:-19716884 MsG0580025506.01.T01:CDS
TGTTTGCTTTGTACGAGCCA+TGG 0.537743 5:+19716909 None:intergenic
GAGAGTTACTATCATGCCTA+AGG 0.550012 5:-19716560 MsG0580025506.01.T01:CDS
ACTTGTTCGTGAAATCGCTC+AGG 0.551065 5:-19716698 MsG0580025506.01.T01:CDS
CAACTCGCTCGCAGAATCAG+AGG 0.556058 5:-19716532 MsG0580025506.01.T01:CDS
TCTTGGTGCCTTTCCTCCGG+TGG 0.556160 5:+19716876 None:intergenic
TTCAGCAGCTTCTTGAAGAG+CGG 0.576635 5:+19716636 None:intergenic
TCGCAGAATCAGAGGCGAGA+GGG 0.584947 5:-19716524 MsG0580025506.01.T01:CDS
CAAACAGCTCGCAAATCCAC+CGG 0.586123 5:-19716892 MsG0580025506.01.T01:CDS
TCACGAACAAGTCTCTGGAA+TGG 0.590006 5:+19716709 None:intergenic
CTCGCAGAATCAGAGGCGAG+AGG 0.599896 5:-19716525 MsG0580025506.01.T01:CDS
AAACCACACAGATTCCGTCC+TGG 0.622196 5:-19716796 MsG0580025506.01.T01:CDS
AAATCTGCTCCAGCAACCGG+AGG 0.664188 5:-19716826 MsG0580025506.01.T01:CDS
GTTCCAGGACGGAATCTGTG+TGG 0.675854 5:+19716793 None:intergenic
GAAGTGCGACAGTTCCAGGA+CGG 0.703486 5:+19716782 None:intergenic
ACAGCTCGCAAATCCACCGG+AGG 0.704783 5:-19716889 MsG0580025506.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATCTTGTTGGTTTGTTTG+AGG - Chr5:19716815-19716834 MsG0580025506.01.T01:CDS 30.0%
AGTAACTCTCTTAGCATGAA+TGG + Chr5:19716856-19716875 None:intergenic 35.0%
GAGAGTTACTATCATGCCTA+AGG - Chr5:19716866-19716885 MsG0580025506.01.T01:CDS 40.0%
ACTTGTTCGTGAAATCGCTC+AGG - Chr5:19716728-19716747 MsG0580025506.01.T01:CDS 45.0%
CAGTGCTCTTCTGATACTTC+CGG + Chr5:19716677-19716696 None:intergenic 45.0%
CGATTTCACGAACAAGTCTC+TGG + Chr5:19716725-19716744 None:intergenic 45.0%
GCTGCTGAAGCTTATCTTGT+TGG - Chr5:19716804-19716823 MsG0580025506.01.T01:CDS 45.0%
TTCAGCAGCTTCTTGAAGAG+CGG + Chr5:19716793-19716812 None:intergenic 45.0%
! CGAGCGAGTTGAATATCCTT+AGG + Chr5:19716885-19716904 None:intergenic 45.0%
! TCACGAACAAGTCTCTGGAA+TGG + Chr5:19716720-19716739 None:intergenic 45.0%
!! AAACAGCGCTGCTTTGGAAT+CGG + Chr5:19716770-19716789 None:intergenic 45.0%
AAACCACACAGATTCCGTCC+TGG - Chr5:19716630-19716649 MsG0580025506.01.T01:CDS 50.0%
CAAACAGCTCGCAAATCCAC+CGG - Chr5:19716534-19716553 MsG0580025506.01.T01:CDS 50.0%
GTGTGGTTTCTTGACTCCTC+CGG + Chr5:19716619-19716638 None:intergenic 50.0%
! TTGGTGGCTAGCTGCTTTCT+TGG + Chr5:19716570-19716589 None:intergenic 50.0%
!! CTTTCTTGGTGCCTTTCCTC+CGG + Chr5:19716556-19716575 None:intergenic 50.0%
AAATCTGCTCCAGCAACCGG+AGG - Chr5:19716600-19716619 MsG0580025506.01.T01:CDS 55.0%
AGCAGATTTCCGGGCAGCTT+TGG + Chr5:19716589-19716608 None:intergenic 55.0%
CAACTCGCTCGCAGAATCAG+AGG - Chr5:19716894-19716913 MsG0580025506.01.T01:CDS 55.0%
CGGAAATCTGCTCCAGCAAC+CGG - Chr5:19716597-19716616 MsG0580025506.01.T01:CDS 55.0%
CGGTTGCTGGAGCAGATTTC+CGG + Chr5:19716599-19716618 None:intergenic 55.0%
CTGTCGCACTTCGTGAGATC+CGG - Chr5:19716655-19716674 MsG0580025506.01.T01:CDS 55.0%
GAAGTGCGACAGTTCCAGGA+CGG + Chr5:19716647-19716666 None:intergenic 55.0%
GGTTGCTGGAGCAGATTTCC+GGG + Chr5:19716598-19716617 None:intergenic 55.0%
GTTCCAGGACGGAATCTGTG+TGG + Chr5:19716636-19716655 None:intergenic 55.0%
TCACGAAGTGCGACAGTTCC+AGG + Chr5:19716651-19716670 None:intergenic 55.0%
TCGCAAATCCACCGGAGGAA+AGG - Chr5:19716542-19716561 MsG0580025506.01.T01:CDS 55.0%
TCGCAGAATCAGAGGCGAGA+GGG - Chr5:19716902-19716921 MsG0580025506.01.T01:CDS 55.0%
TTCTTGACTCCTCCGGTTGC+TGG + Chr5:19716612-19716631 None:intergenic 55.0%
! AGATTTCCGGGCAGCTTTGG+TGG + Chr5:19716586-19716605 None:intergenic 55.0%
ACAGCTCGCAAATCCACCGG+AGG - Chr5:19716537-19716556 MsG0580025506.01.T01:CDS 60.0%
AGCTAGCCACCAAAGCTGCC+CGG - Chr5:19716577-19716596 MsG0580025506.01.T01:CDS 60.0%
CTCGCAGAATCAGAGGCGAG+AGG - Chr5:19716901-19716920 MsG0580025506.01.T01:CDS 60.0%
GAGCGGAAACAGCGCTGCTT+TGG + Chr5:19716776-19716795 None:intergenic 60.0%
!! TCTTGGTGCCTTTCCTCCGG+TGG + Chr5:19716553-19716572 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 19716519 19716929 19716519 ID=MsG0580025506.01;Name=MsG0580025506.01
Chr5 mRNA 19716519 19716929 19716519 ID=MsG0580025506.01.T01;Parent=MsG0580025506.01;Name=MsG0580025506.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|136
Chr5 exon 19716519 19716929 19716519 ID=MsG0580025506.01.T01:exon:8331;Parent=MsG0580025506.01.T01
Chr5 CDS 19716519 19716929 19716519 ID=MsG0580025506.01.T01:cds;Parent=MsG0580025506.01.T01
Gene Sequence

>MsG0580025506.01.T01

ATGGCTCGTACAAAGCAAACAGCTCGCAAATCCACCGGAGGAAAGGCACCAAGAAAGCAGCTAGCCACCAAAGCTGCCCGGAAATCTGCTCCAGCAACCGGAGGAGTCAAGAAACCACACAGATTCCGTCCTGGAACTGTCGCACTTCGTGAGATCCGGAAGTATCAGAAGAGCACTGAGCTTCTCATAAGAAAGCTTCCATTCCAGAGACTTGTTCGTGAAATCGCTCAGGATTTCAAAACAGATCTCCGATTCCAAAGCAGCGCTGTTTCCGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAGGATACTAATCTCTGTGCCATTCATGCTAAGAGAGTTACTATCATGCCTAAGGATATTCAACTCGCTCGCAGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>MsG0580025506.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*