Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017848.01.T01 | AEX20345.1 | 87.069 | 116 | 15 | 0 | 8 | 123 | 351 | 466 | 2.69E-61 | 204 |
| MsG0380017848.01.T01 | AEX20345.1 | 83.333 | 48 | 8 | 0 | 8 | 55 | 291 | 338 | 1.29E-15 | 83.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017848.01.T01 | H2EIY5 | 87.069 | 116 | 15 | 0 | 8 | 123 | 351 | 466 | 1.29e-61 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002089.01 | MsG0380017848.01 | 0.809908 | 1.499683e-50 | 8.915296e-48 |
| MsG0380011870.01 | MsG0380017848.01 | 0.808268 | 3.366767e-50 | 1.916569e-47 |
| MsG0380016160.01 | MsG0380017848.01 | -0.814444 | 1.535749e-51 | 1.030657e-48 |
| MsG0380017848.01 | MsG0580027687.01 | 0.827257 | 1.728522e-54 | 1.660058e-51 |
| MsG0380017848.01 | MsG0580027688.01 | 0.838099 | 3.517113e-57 | 4.661969e-54 |
| MsG0380017848.01 | MsG0580029204.01 | 0.809256 | 2.070261e-50 | 1.209628e-47 |
| MsG0380017848.01 | MsG0680032801.01 | 0.807453 | 5.017299e-50 | 2.795744e-47 |
| MsG0380017848.01 | MsG0680033379.01 | 0.808882 | 2.489502e-50 | 1.440034e-47 |
| MsG0380017848.01 | MsG0880042454.01 | 0.811372 | 7.236368e-51 | 4.471500e-48 |
| MsG0280009646.01 | MsG0380017848.01 | 0.817733 | 2.828831e-52 | 2.076917e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017848.01.T01 | MTR_3g114180 | 86.239 | 109 | 15 | 0 | 8 | 116 | 24 | 132 | 2.26e-64 | 192 |
| MsG0380017848.01.T01 | MTR_3g114190 | 86.239 | 109 | 15 | 0 | 8 | 116 | 24 | 132 | 1.55e-61 | 192 |
| MsG0380017848.01.T01 | MTR_3g114190 | 86.239 | 109 | 15 | 0 | 8 | 116 | 254 | 362 | 1.55e-61 | 192 |
| MsG0380017848.01.T01 | MTR_3g113780 | 87.755 | 98 | 12 | 0 | 8 | 105 | 13 | 110 | 6.06e-59 | 178 |
| MsG0380017848.01.T01 | MTR_3g113780 | 87.838 | 74 | 9 | 0 | 8 | 81 | 13 | 86 | 4.31e-43 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACGTCTTTGCGTAAAT+TGG | 0.114766 | 3:+101386537 | None:intergenic |
| CTTGCCTTATCAGGTATAAT+TGG | 0.191560 | 3:+101386358 | None:intergenic |
| GCTCCTTTGGCTTCTTCCCA+TGG | 0.393602 | 3:+101386625 | None:intergenic |
| CCAACGTCTTTGCGTAAATT+GGG | 0.410676 | 3:+101386538 | None:intergenic |
| TCTCTAACAGCATGCATACT+TGG | 0.418938 | 3:-101386269 | MsG0380017848.01.T01:intron |
| AAAGAGGAGCTGTTACTGAT+AGG | 0.462033 | 3:-101386148 | MsG0380017848.01.T01:CDS |
| GTGTCCAATTATACCTGATA+AGG | 0.474662 | 3:-101386362 | MsG0380017848.01.T01:intron |
| GAGGGAGAGCTTCTTTATGA+TGG | 0.519007 | 3:-101386585 | MsG0380017848.01.T01:CDS |
| GCGTCTTGAGAAAGGGAAAG+AGG | 0.542494 | 3:-101386164 | MsG0380017848.01.T01:CDS |
| CCCAATTTACGCAAAGACGT+TGG | 0.542545 | 3:-101386538 | MsG0380017848.01.T01:CDS |
| ACAAAGGAGGATCTTCTCCA+TGG | 0.556428 | 3:-101386642 | MsG0380017848.01.T01:CDS |
| TAGATACAATAGACCTCAAG+TGG | 0.580845 | 3:-101386384 | MsG0380017848.01.T01:CDS |
| AAAAGTAGAAAGCACAAAGG+AGG | 0.607683 | 3:-101386655 | MsG0380017848.01.T01:CDS |
| CAAAGGAGGATCTTCTCCAT+GGG | 0.608293 | 3:-101386641 | MsG0380017848.01.T01:CDS |
| ATGCAGAGGAAAAGAAGCAG+TGG | 0.630680 | 3:-101386210 | MsG0380017848.01.T01:CDS |
| GTATAATTGGACACCACTTG+AGG | 0.635018 | 3:+101386371 | None:intergenic |
| TCTCCATGGGAAGAAGCCAA+AGG | 0.672590 | 3:-101386628 | MsG0380017848.01.T01:CDS |
| ACTCTTCAAGAAGCATGCAG+AGG | 0.682031 | 3:-101386224 | MsG0380017848.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTGCATATTTTTATTCTTT+AGG | - | Chr3:101386524-101386543 | MsG0380017848.01.T01:intron | 20.0% |
| ! | GTTTCTTCTCTTGTTTCATA+TGG | - | Chr3:101386385-101386404 | MsG0380017848.01.T01:CDS | 30.0% |
| !! | CAAAAAGTAGAAAGTACTGA+GGG | - | Chr3:101386222-101386241 | MsG0380017848.01.T01:CDS | 30.0% |
| !!! | ATTGGGCAATCGAATTTTTT+TGG | + | Chr3:101386273-101386292 | None:intergenic | 30.0% |
| AGAGAAAAACTTGCCTTATC+AGG | + | Chr3:101386479-101386498 | None:intergenic | 35.0% | |
| CTTGCCTTATCAGGTATAAT+TGG | + | Chr3:101386470-101386489 | None:intergenic | 35.0% | |
| GTGTCCAATTATACCTGATA+AGG | - | Chr3:101386463-101386482 | MsG0380017848.01.T01:intron | 35.0% | |
| TAGATACAATAGACCTCAAG+TGG | - | Chr3:101386441-101386460 | MsG0380017848.01.T01:intron | 35.0% | |
| ! | AAAAGTAGAAAGCACAAAGG+AGG | - | Chr3:101386170-101386189 | MsG0380017848.01.T01:CDS | 35.0% |
| ! | GCAAAAAGTAGAAAGCACAA+AGG | - | Chr3:101386167-101386186 | MsG0380017848.01.T01:CDS | 35.0% |
| !! | AAAAAGAGCGTCTTGAGAAA+GGG | - | Chr3:101386654-101386673 | MsG0380017848.01.T01:CDS | 35.0% |
| !! | GCAAAAAGTAGAAAGTACTG+AGG | - | Chr3:101386221-101386240 | MsG0380017848.01.T01:CDS | 35.0% |
| !!! | CTTTCTACTTTTTGCTCCTT+TGG | + | Chr3:101386216-101386235 | None:intergenic | 35.0% |
| AAAGAGGAGCTGTTACTGAT+AGG | - | Chr3:101386677-101386696 | MsG0380017848.01.T01:CDS | 40.0% | |
| CCAACGTCTTTGCGTAAATT+GGG | + | Chr3:101386290-101386309 | None:intergenic | 40.0% | |
| GAAGAAACAACCATAAGCTG+AGG | + | Chr3:101386373-101386392 | None:intergenic | 40.0% | |
| GGTCAAATAACCTCAGCTTA+TGG | - | Chr3:101386360-101386379 | MsG0380017848.01.T01:intron | 40.0% | |
| GTATAATTGGACACCACTTG+AGG | + | Chr3:101386457-101386476 | None:intergenic | 40.0% | |
| TCCAACGTCTTTGCGTAAAT+TGG | + | Chr3:101386291-101386310 | None:intergenic | 40.0% | |
| TCTCTAACAGCATGCATACT+TGG | - | Chr3:101386556-101386575 | MsG0380017848.01.T01:CDS | 40.0% | |
| !! | CTCTCTTTGCTGTTTGCATT+TGG | - | Chr3:101386339-101386358 | MsG0380017848.01.T01:intron | 40.0% |
| !! | GAAAAAGAGCGTCTTGAGAA+AGG | - | Chr3:101386653-101386672 | MsG0380017848.01.T01:CDS | 40.0% |
| ACAAAGGAGGATCTTCTCCA+TGG | - | Chr3:101386183-101386202 | MsG0380017848.01.T01:CDS | 45.0% | |
| AGAGAGACAAAGAGAGAGAG+AGG | + | Chr3:101386325-101386344 | None:intergenic | 45.0% | |
| ATGCAGAGGAAAAGAAGCAG+TGG | - | Chr3:101386615-101386634 | MsG0380017848.01.T01:CDS | 45.0% | |
| CAAAGGAGGATCTTCTCCAT+GGG | - | Chr3:101386184-101386203 | MsG0380017848.01.T01:CDS | 45.0% | |
| CCCAATTTACGCAAAGACGT+TGG | - | Chr3:101386287-101386306 | MsG0380017848.01.T01:intron | 45.0% | |
| GAGAGACAAAGAGAGAGAGA+GGG | + | Chr3:101386324-101386343 | None:intergenic | 45.0% | |
| GAGGGAGAGCTTCTTTATGA+TGG | - | Chr3:101386240-101386259 | MsG0380017848.01.T01:CDS | 45.0% | |
| ! | ACTCTTCAAGAAGCATGCAG+AGG | - | Chr3:101386601-101386620 | MsG0380017848.01.T01:CDS | 45.0% |
| GCGTCTTGAGAAAGGGAAAG+AGG | - | Chr3:101386661-101386680 | MsG0380017848.01.T01:CDS | 50.0% | |
| TCTCCATGGGAAGAAGCCAA+AGG | - | Chr3:101386197-101386216 | MsG0380017848.01.T01:CDS | 50.0% | |
| ! | GCTCCTTTGGCTTCTTCCCA+TGG | + | Chr3:101386203-101386222 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 101386144 | 101386703 | 101386144 | ID=MsG0380017848.01;Name=MsG0380017848.01 |
| Chr3 | mRNA | 101386144 | 101386703 | 101386144 | ID=MsG0380017848.01.T01;Parent=MsG0380017848.01;Name=MsG0380017848.01.T01;_AED=0.11;_eAED=0.31;_QI=0|0|0|1|0|0|3|0|123 |
| Chr3 | exon | 101386144 | 101386281 | 101386144 | ID=MsG0380017848.01.T01:exon:18160;Parent=MsG0380017848.01.T01 |
| Chr3 | exon | 101386363 | 101386427 | 101386363 | ID=MsG0380017848.01.T01:exon:18159;Parent=MsG0380017848.01.T01 |
| Chr3 | exon | 101386535 | 101386703 | 101386535 | ID=MsG0380017848.01.T01:exon:18158;Parent=MsG0380017848.01.T01 |
| Chr3 | CDS | 101386535 | 101386703 | 101386535 | ID=MsG0380017848.01.T01:cds;Parent=MsG0380017848.01.T01 |
| Chr3 | CDS | 101386363 | 101386427 | 101386363 | ID=MsG0380017848.01.T01:cds;Parent=MsG0380017848.01.T01 |
| Chr3 | CDS | 101386144 | 101386281 | 101386144 | ID=MsG0380017848.01.T01:cds;Parent=MsG0380017848.01.T01 |
| Gene Sequence |
| Protein sequence |
