AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029204.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029204.01.T01 MTR_5g082780 82.609 230 22 2 98 309 1 230 2.50e-130 372
MsG0580029204.01.T01 MTR_5g082770 61.644 292 84 4 1 266 1 290 7.73e-115 335
MsG0580029204.01.T01 MTR_5g038230 47.339 357 168 9 1 341 1 353 1.55e-99 298
MsG0580029204.01.T01 MTR_5g038260 44.241 382 169 10 1 341 3 381 8.15e-96 290
MsG0580029204.01.T01 MTR_1g064570 42.000 400 171 9 1 341 1 398 9.55e-93 282
MsG0580029204.01.T01 MTR_1g108740 42.049 371 151 11 4 341 5 344 1.15e-78 244
MsG0580029204.01.T01 MTR_7g077660 40.103 389 157 15 3 341 4 366 3.20e-71 226
MsG0580029204.01.T01 MTR_7g077650 38.987 395 157 15 2 340 3 369 7.93e-70 223
MsG0580029204.01.T01 MTR_7g077620 39.541 392 161 13 2 341 3 370 6.31e-68 218
MsG0580029204.01.T01 MTR_1g016150 40.404 297 90 8 4 278 5 236 3.60e-57 186
MsG0580029204.01.T01 MTR_7g076100 33.718 347 169 13 3 341 5 298 1.21e-38 139
MsG0580029204.01.T01 MTR_7g076110 33.548 310 145 11 73 341 22 311 1.53e-34 129
MsG0580029204.01.T01 MTR_1g108750 44.371 151 56 3 1 150 1 124 6.91e-26 100
MsG0580029204.01.T01 MTR_3g114990 24.505 404 225 19 1 339 20 408 2.69e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAATATCATTGGAAATT+TGG 0.116569 5:+93668833 None:intergenic
TAGCATTTGTTTAAACTTTC+CGG 0.163534 5:+93669114 None:intergenic
GGAACTAGATTGGGCAAATT+TGG 0.170985 5:-93669895 MsG0580029204.01.T01:CDS
TAAGAATTTGGCAATTTAAA+TGG 0.183830 5:+93669672 None:intergenic
TAATCATTTGGTCTTGTTAT+AGG 0.187600 5:+93669477 None:intergenic
TACAAACATCTTTCTGTTCT+TGG 0.199967 5:+93669720 None:intergenic
AGCAGCAGCCACCACCTTTC+TGG 0.214961 5:-93668862 MsG0580029204.01.T01:CDS
TTTATGGATGTTATGTTCTA+TGG 0.218295 5:-93669357 MsG0580029204.01.T01:CDS
GAGCTCGCTTCATTCCTTTA+TGG 0.232283 5:-93669373 MsG0580029204.01.T01:CDS
GGCTAAATCCACAGCAAATA+TGG 0.248424 5:+93669646 None:intergenic
CAAATATGGTAATAAGAATT+TGG 0.264290 5:+93669660 None:intergenic
AATCATTTGGTCTTGTTATA+GGG 0.294407 5:+93669478 None:intergenic
AACAAGACCAAATGATTATA+TGG 0.314220 5:-93669472 MsG0580029204.01.T01:CDS
ACTTATGGTCCATTTCATAT+AGG 0.327197 5:-93668947 MsG0580029204.01.T01:CDS
CTTATGGTCCATTTCATATA+GGG 0.341132 5:-93668946 MsG0580029204.01.T01:CDS
TCGAAGGTTGGAAGACTAAT+AGG 0.348536 5:+93669555 None:intergenic
AAGTTAGATTGTGTAACTTA+TGG 0.369088 5:-93668962 MsG0580029204.01.T01:CDS
AGAAGGATGCAATATTGTTA+TGG 0.378560 5:+93669590 None:intergenic
ACAAACATCTTTCTGTTCTT+GGG 0.381418 5:+93669721 None:intergenic
GTATCAGCTTCATACTTCTC+TGG 0.384518 5:-93669034 MsG0580029204.01.T01:CDS
GCCACAACCATATAATCATT+TGG 0.389176 5:+93669465 None:intergenic
GCAGCAGCCACCACCTTTCT+GGG 0.398247 5:-93668861 MsG0580029204.01.T01:CDS
TGGTTAGAATGGAACTAGAT+TGG 0.415806 5:-93669905 None:intergenic
TTATTTAATTCTTGATAAGT+TGG 0.416195 5:-93669859 MsG0580029204.01.T01:CDS
GGTTAGAATGGAACTAGATT+GGG 0.421888 5:-93669904 None:intergenic
TTATATATCCCTATATGAAA+TGG 0.424795 5:+93668938 None:intergenic
CGAATTCAAACTTGTACACT+TGG 0.434747 5:+93669146 None:intergenic
TTATCTTGTGAAATCATTAG+AGG 0.436221 5:-93669215 MsG0580029204.01.T01:CDS
ATAGGAGCAACATTCTTAGA+AGG 0.444870 5:+93669573 None:intergenic
ATAGAACATAACATCCATAA+AGG 0.458967 5:+93669359 None:intergenic
TGTTCTTGGGATCATTAGCA+TGG 0.462460 5:+93669734 None:intergenic
TTGGTGCCGTGTGCAAGAAT+TGG 0.464467 5:-93669812 MsG0580029204.01.T01:CDS
ATGCTAAAGCTTGAGAGTCT+TGG 0.465110 5:-93669097 MsG0580029204.01.T01:CDS
TAAAAGCGGGCGAACCATGC+TGG 0.470666 5:-93669407 MsG0580029204.01.T01:CDS
ACCAAATGATTATATGGTTG+TGG 0.479866 5:-93669466 MsG0580029204.01.T01:CDS
AACCATGCTGGACTTATGTA+TGG 0.481402 5:-93669395 MsG0580029204.01.T01:CDS
TTTATCTGAGGTCGGGTACA+AGG 0.517119 5:-93669517 MsG0580029204.01.T01:CDS
TCTTATTACCATATTTGCTG+TGG 0.524145 5:-93669654 MsG0580029204.01.T01:CDS
TTTGAATTCGATGCTCATAC+CGG 0.528075 5:-93669133 MsG0580029204.01.T01:CDS
GTGAAGATTATGATGATCAA+TGG 0.528142 5:+93668901 None:intergenic
ATACAAGTTTATCTGAGGTC+GGG 0.533775 5:-93669524 MsG0580029204.01.T01:CDS
GGGATATATAATGTAAGAGA+TGG 0.535003 5:-93668926 MsG0580029204.01.T01:CDS
TATACAAGTTTATCTGAGGT+CGG 0.543905 5:-93669525 MsG0580029204.01.T01:CDS
ATGGATGTTATGTTCTATGG+AGG 0.548376 5:-93669354 MsG0580029204.01.T01:CDS
AAATTTGGTAAAACCCAGAA+AGG 0.548520 5:+93668848 None:intergenic
GTTCTTGGGATCATTAGCAT+GGG 0.554796 5:+93669735 None:intergenic
ATTTGCTGTGGATTTAGCCA+TGG 0.559735 5:-93669642 MsG0580029204.01.T01:CDS
TATCTTGTGAAATCATTAGA+GGG 0.568311 5:-93669214 MsG0580029204.01.T01:CDS
GGTAAAACCCAGAAAGGTGG+TGG 0.590899 5:+93668854 None:intergenic
GTATATGATCGATCGAAGGT+TGG 0.610922 5:+93669543 None:intergenic
ATCATATACAAGTTTATCTG+AGG 0.615631 5:-93669529 MsG0580029204.01.T01:CDS
CTCCATACATAAGTCCAGCA+TGG 0.636983 5:+93669393 None:intergenic
AATCATTAGAGGGAGACTTG+TGG 0.640892 5:-93669204 MsG0580029204.01.T01:CDS
TTTGGTAAAACCCAGAAAGG+TGG 0.643098 5:+93668851 None:intergenic
TAATCGCCAATTCTTGCACA+CGG 0.645036 5:+93669806 None:intergenic
AGCTTAACCATCAAAATCAG+CGG 0.648160 5:-93669776 MsG0580029204.01.T01:CDS
TATGTTGCGAGTAAATATCA+CGG 0.663367 5:-93669324 MsG0580029204.01.T01:intron
ACTTGTATATGATCGATCGA+AGG 0.668705 5:+93669539 None:intergenic
ATTAGAGGGAGACTTGTGGA+TGG 0.690554 5:-93669200 MsG0580029204.01.T01:CDS
CTATTGTTGCAAGACAACCA+TGG 0.694642 5:+93669625 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTAATTCTTGATAAGT+TGG - Chr5:93668879-93668898 MsG0580029204.01.T01:CDS 15.0%
!!! GGTATAATATCTTTTAATAT+TGG - Chr5:93669435-93669454 MsG0580029204.01.T01:CDS 15.0%
!!! TGAAAAGATTTTTTACTTTT+AGG - Chr5:93669561-93669580 MsG0580029204.01.T01:CDS 15.0%
!! CAAATATGGTAATAAGAATT+TGG + Chr5:93669081-93669100 None:intergenic 20.0%
!! TAAGAATTTGGCAATTTAAA+TGG + Chr5:93669069-93669088 None:intergenic 20.0%
!! TTATATATCCCTATATGAAA+TGG + Chr5:93669803-93669822 None:intergenic 20.0%
!!! CTTTTAATATTGGTCATAAA+AGG - Chr5:93669445-93669464 MsG0580029204.01.T01:CDS 20.0%
!!! TTTAAATGGATACATTTTCT+TGG + Chr5:93669055-93669074 None:intergenic 20.0%
! AACAAGACCAAATGATTATA+TGG - Chr5:93669266-93669285 MsG0580029204.01.T01:intron 25.0%
! AAGTTAGATTGTGTAACTTA+TGG - Chr5:93669776-93669795 MsG0580029204.01.T01:CDS 25.0%
! ATAGAACATAACATCCATAA+AGG + Chr5:93669382-93669401 None:intergenic 25.0%
! ATCATATACAAGTTTATCTG+AGG - Chr5:93669209-93669228 MsG0580029204.01.T01:CDS 25.0%
! TAGCATTTGTTTAAACTTTC+CGG + Chr5:93669627-93669646 None:intergenic 25.0%
! TATCTTGTGAAATCATTAGA+GGG - Chr5:93669524-93669543 MsG0580029204.01.T01:CDS 25.0%
! TTATCTTGTGAAATCATTAG+AGG - Chr5:93669523-93669542 MsG0580029204.01.T01:CDS 25.0%
! TTTATGGATGTTATGTTCTA+TGG - Chr5:93669381-93669400 MsG0580029204.01.T01:CDS 25.0%
!!! AATCATTTGGTCTTGTTATA+GGG + Chr5:93669263-93669282 None:intergenic 25.0%
!!! CAAATAGATCACGTTTTTTT+TGG - Chr5:93668907-93668926 MsG0580029204.01.T01:CDS 25.0%
!!! TAATCATTTGGTCTTGTTAT+AGG + Chr5:93669264-93669283 None:intergenic 25.0%
AAATTTGGTAAAACCCAGAA+AGG + Chr5:93669893-93669912 None:intergenic 30.0%
ACAAACATCTTTCTGTTCTT+GGG + Chr5:93669020-93669039 None:intergenic 30.0%
ACCAAATGATTATATGGTTG+TGG - Chr5:93669272-93669291 MsG0580029204.01.T01:intron 30.0%
ACTTATGGTCCATTTCATAT+AGG - Chr5:93669791-93669810 MsG0580029204.01.T01:CDS 30.0%
AGAAGGATGCAATATTGTTA+TGG + Chr5:93669151-93669170 None:intergenic 30.0%
CTTATGGTCCATTTCATATA+GGG - Chr5:93669792-93669811 MsG0580029204.01.T01:CDS 30.0%
GGGATATATAATGTAAGAGA+TGG - Chr5:93669812-93669831 MsG0580029204.01.T01:CDS 30.0%
GTGAAGATTATGATGATCAA+TGG + Chr5:93669840-93669859 None:intergenic 30.0%
TACAAACATCTTTCTGTTCT+TGG + Chr5:93669021-93669040 None:intergenic 30.0%
TATGTTGCGAGTAAATATCA+CGG - Chr5:93669414-93669433 MsG0580029204.01.T01:CDS 30.0%
! TATACAAGTTTATCTGAGGT+CGG - Chr5:93669213-93669232 MsG0580029204.01.T01:CDS 30.0%
! TCTTATTACCATATTTGCTG+TGG - Chr5:93669084-93669103 MsG0580029204.01.T01:CDS 30.0%
!! ACGTTTCGCTTTTATAAAAG+CGG - Chr5:93669317-93669336 MsG0580029204.01.T01:intron 30.0%
!! GGAGACAATGTTTTATTCTT+GGG - Chr5:93669662-93669681 MsG0580029204.01.T01:CDS 30.0%
!! TGGAGACAATGTTTTATTCT+TGG - Chr5:93669661-93669680 MsG0580029204.01.T01:CDS 30.0%
ACTTGTATATGATCGATCGA+AGG + Chr5:93669202-93669221 None:intergenic 35.0%
AGCTTAACCATCAAAATCAG+CGG - Chr5:93668962-93668981 MsG0580029204.01.T01:CDS 35.0%
ATAGGAGCAACATTCTTAGA+AGG + Chr5:93669168-93669187 None:intergenic 35.0%
ATGGATGTTATGTTCTATGG+AGG - Chr5:93669384-93669403 MsG0580029204.01.T01:CDS 35.0%
CGAATTCAAACTTGTACACT+TGG + Chr5:93669595-93669614 None:intergenic 35.0%
GATAACTCCAAATCCAATTC+AGG - Chr5:93669467-93669486 MsG0580029204.01.T01:CDS 35.0%
GCCACAACCATATAATCATT+TGG + Chr5:93669276-93669295 None:intergenic 35.0%
TTTGAATTCGATGCTCATAC+CGG - Chr5:93669605-93669624 MsG0580029204.01.T01:CDS 35.0%
! ATACAAGTTTATCTGAGGTC+GGG - Chr5:93669214-93669233 MsG0580029204.01.T01:CDS 35.0%
! GCTTTTCATGGAAGGTTTAA+AGG - Chr5:93669500-93669519 MsG0580029204.01.T01:CDS 35.0%
!! CGTTTCGCTTTTATAAAAGC+GGG - Chr5:93669318-93669337 MsG0580029204.01.T01:intron 35.0%
AACCATGCTGGACTTATGTA+TGG - Chr5:93669343-93669362 MsG0580029204.01.T01:CDS 40.0%
AATCATTAGAGGGAGACTTG+TGG - Chr5:93669534-93669553 MsG0580029204.01.T01:CDS 40.0%
ATGCTAAAGCTTGAGAGTCT+TGG - Chr5:93669641-93669660 MsG0580029204.01.T01:CDS 40.0%
CTATTGTTGCAAGACAACCA+TGG + Chr5:93669116-93669135 None:intergenic 40.0%
GGAACTAGATTGGGCAAATT+TGG - Chr5:93668843-93668862 MsG0580029204.01.T01:CDS 40.0%
GGCTAAATCCACAGCAAATA+TGG + Chr5:93669095-93669114 None:intergenic 40.0%
GTATATGATCGATCGAAGGT+TGG + Chr5:93669198-93669217 None:intergenic 40.0%
GTATCAGCTTCATACTTCTC+TGG - Chr5:93669704-93669723 MsG0580029204.01.T01:CDS 40.0%
GTTCTTGGGATCATTAGCAT+GGG + Chr5:93669006-93669025 None:intergenic 40.0%
TAATCGCCAATTCTTGCACA+CGG + Chr5:93668935-93668954 None:intergenic 40.0%
TCCAAATCCAATTCAGGACA+TGG - Chr5:93669473-93669492 MsG0580029204.01.T01:CDS 40.0%
TCGAAGGTTGGAAGACTAAT+AGG + Chr5:93669186-93669205 None:intergenic 40.0%
TGTTCTTGGGATCATTAGCA+TGG + Chr5:93669007-93669026 None:intergenic 40.0%
TTTGGTAAAACCCAGAAAGG+TGG + Chr5:93669890-93669909 None:intergenic 40.0%
! ATGGCATAGCTTTTCATGGA+AGG - Chr5:93669492-93669511 MsG0580029204.01.T01:CDS 40.0%
!! ATTTGCTGTGGATTTAGCCA+TGG - Chr5:93669096-93669115 MsG0580029204.01.T01:CDS 40.0%
!!! TTCTGAACCGCTGATTTTGA+TGG + Chr5:93668972-93668991 None:intergenic 40.0%
AGCTATGCCATGTCCTGAAT+TGG + Chr5:93669483-93669502 None:intergenic 45.0%
ATTAGAGGGAGACTTGTGGA+TGG - Chr5:93669538-93669557 MsG0580029204.01.T01:CDS 45.0%
CTCCATACATAAGTCCAGCA+TGG + Chr5:93669348-93669367 None:intergenic 45.0%
GAGCTCGCTTCATTCCTTTA+TGG - Chr5:93669365-93669384 MsG0580029204.01.T01:CDS 45.0%
GCCATGTCCTGAATTGGATT+TGG + Chr5:93669477-93669496 None:intergenic 45.0%
! GGACATGGCATAGCTTTTCA+TGG - Chr5:93669488-93669507 MsG0580029204.01.T01:CDS 45.0%
! TTTATCTGAGGTCGGGTACA+AGG - Chr5:93669221-93669240 MsG0580029204.01.T01:CDS 45.0%
GGTAAAACCCAGAAAGGTGG+TGG + Chr5:93669887-93669906 None:intergenic 50.0%
!! TTGGTGCCGTGTGCAAGAAT+TGG - Chr5:93668926-93668945 MsG0580029204.01.T01:CDS 50.0%
TAAAAGCGGGCGAACCATGC+TGG - Chr5:93669331-93669350 MsG0580029204.01.T01:CDS 55.0%
AGCAGCAGCCACCACCTTTC+TGG - Chr5:93669876-93669895 MsG0580029204.01.T01:CDS 60.0%
GCAGCAGCCACCACCTTTCT+GGG - Chr5:93669877-93669896 MsG0580029204.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 93668841 93669919 93668841 ID=MsG0580029204.01;Name=MsG0580029204.01
Chr5 mRNA 93668841 93669919 93668841 ID=MsG0580029204.01.T01;Parent=MsG0580029204.01;Name=MsG0580029204.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|0.5|2|0|341
Chr5 exon 93669325 93669919 93669325 ID=MsG0580029204.01.T01:exon:49692;Parent=MsG0580029204.01.T01
Chr5 exon 93668841 93669271 93668841 ID=MsG0580029204.01.T01:exon:49691;Parent=MsG0580029204.01.T01
Chr5 CDS 93669325 93669919 93669325 ID=MsG0580029204.01.T01:cds;Parent=MsG0580029204.01.T01
Chr5 CDS 93668841 93669271 93668841 ID=MsG0580029204.01.T01:cds;Parent=MsG0580029204.01.T01
Gene Sequence

>MsG0580029204.01.T01

ATGGAACTAGATTGGGCAAATTTGGAAGCACTTGCACTTTATTTAATTCTTGATAAGTTGGAAGAACAAATAGATCACGTTTTTTTTGGTGCCGTGTGCAAGAATTGGCGATTAATTGCAAAGCTTAACCATCAAAATCAGCGGTTCAGAACTCATGTACTACCCATGCTAATGATCCCAAGAACAGAAAGATGTTTGTATAGCATTTCAGCCAAGAAAATGTATCCATTTAAATTGCCAAATTCTTATTACCATATTTGCTGTGGATTTAGCCATGGTTGTCTTGCAACAATAGATAAAAGTCATGCCATAACAATATTGCATCCTTCTAAGAATGTTGCTCCTATTAGTCTTCCAACCTTCGATCGATCATATACAAGTTTATCTGAGGTCGGGTACAAGGTCACTCTTTCTGCTGACCCTATAACAAGACCAAATGATTATATGGTTGTGGCAATTCACACTAGCATTCATGAACGTTTCGCTTTTATAAAAGCGGGCGAACCATGCTGGACTTATGTATGGAGCTCGCTTCATTCCTTTATGGATGTTATGTTCTATGGAGGTTTAGTTTATGTTGCGAGTAAATATCACGGACATGGCATAGCTTTTCATGGAAGGTTTAAAGGTTATCTTGTGAAATCATTAGAGGGAGACTTGTGGATGGTGAAAAGATTTTTTACTTTTAGGAATGTTTTCCAAGTGTACAAGTTTGAATTCGATGCTCATACCGGAAAGTTTAAACAAATGCTAAAGCTTGAGAGTCTTGGAGACAATGTTTTATTCTTGGGAGACACTGATTCAATTTCTGTATCAGCTTCATACTTCTCTGGTTGTCTGAAAAAAGATTCAATTTATTACACAGAAAGTATTAATGCAAACAAGTTAGATTGTGTAACTTATGGTCCATTTCATATAGGGATATATAATGTAAGAGATGGAACCATTGATCATCATAATCTTCACAATCATTTCTTCAACAAGCAGCAGCCACCACCTTTCTGGGTTTTACCAAATTTCCAATGA

Protein sequence

>MsG0580029204.01.T01

MELDWANLEALALYLILDKLEEQIDHVFFGAVCKNWRLIAKLNHQNQRFRTHVLPMLMIPRTERCLYSISAKKMYPFKLPNSYYHICCGFSHGCLATIDKSHAITILHPSKNVAPISLPTFDRSYTSLSEVGYKVTLSADPITRPNDYMVVAIHTSIHERFAFIKAGEPCWTYVWSSLHSFMDVMFYGGLVYVASKYHGHGIAFHGRFKGYLVKSLEGDLWMVKRFFTFRNVFQVYKFEFDAHTGKFKQMLKLESLGDNVLFLGDTDSISVSASYFSGCLKKDSIYYTESINANKLDCVTYGPFHIGIYNVRDGTIDHHNLHNHFFNKQQPPPFWVLPNFQ*