Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017879.01.T05 | AES74062.2 | 92.381 | 315 | 23 | 1 | 1 | 315 | 64 | 377 | 0 | 566 |
| MsG0380017879.01.T06 | AES74062.2 | 93.08 | 289 | 19 | 1 | 1 | 289 | 90 | 377 | 0 | 543 |
| MsG0380017879.01.T01 | XP_003603811.3 | 91.512 | 377 | 31 | 1 | 1 | 377 | 1 | 376 | 0 | 675 |
| MsG0380017879.01.T03 | AES74062.2 | 87.302 | 378 | 30 | 2 | 1 | 361 | 1 | 377 | 0 | 632 |
| MsG0380017879.01.T02 | AES74062.2 | 88.889 | 378 | 28 | 2 | 1 | 365 | 1 | 377 | 0 | 646 |
| MsG0380017879.01.T04 | AES74062.2 | 91.799 | 378 | 30 | 1 | 1 | 378 | 1 | 377 | 0 | 678 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017879.01.T01 | Q6DYE5 | 39.617 | 366 | 200 | 7 | 26 | 376 | 29 | 388 | 1.34E-67 | 221 |
| MsG0380017879.01.T02 | Q6DYE5 | 39.56 | 364 | 186 | 8 | 27 | 362 | 29 | 386 | 2.36E-65 | 215 |
| MsG0380017879.01.T03 | Q6DYE5 | 39.669 | 363 | 183 | 8 | 27 | 358 | 29 | 386 | 7.72E-65 | 213 |
| MsG0380017879.01.T04 | Q6DYE5 | 39.617 | 366 | 200 | 7 | 27 | 377 | 29 | 388 | 2.29E-67 | 221 |
| MsG0380017879.01.T05 | Q6DYE5 | 39.803 | 304 | 172 | 5 | 16 | 314 | 91 | 388 | 1.29E-61 | 203 |
| MsG0380017879.01.T06 | Q6DYE5 | 40.702 | 285 | 158 | 5 | 9 | 288 | 110 | 388 | 2.14E-58 | 194 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017879.01.T01 | A0A396J381 | 91.512 | 377 | 31 | 1 | 1 | 377 | 1 | 376 | 0.0 | 675 |
| MsG0380017879.01.T02 | G7J5U4 | 88.889 | 378 | 28 | 2 | 1 | 365 | 1 | 377 | 0.0 | 646 |
| MsG0380017879.01.T03 | G7J5U4 | 87.302 | 378 | 30 | 2 | 1 | 361 | 1 | 377 | 0.0 | 632 |
| MsG0380017879.01.T04 | G7J5U4 | 91.799 | 378 | 30 | 1 | 1 | 378 | 1 | 377 | 0.0 | 678 |
| MsG0380017879.01.T05 | G7J5U4 | 92.381 | 315 | 23 | 1 | 1 | 315 | 64 | 377 | 0.0 | 566 |
| MsG0380017879.01.T06 | G7J5U4 | 93.080 | 289 | 19 | 1 | 1 | 289 | 90 | 377 | 0.0 | 543 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048915.01 | MsG0380017879.01 | 0.812351 | 4.429664e-51 | 2.809956e-48 |
| MsG0280010784.01 | MsG0380017879.01 | 0.815356 | 9.642748e-52 | 6.633226e-49 |
| MsG0280011024.01 | MsG0380017879.01 | 0.814091 | 1.837727e-51 | 1.221524e-48 |
| MsG0280011131.01 | MsG0380017879.01 | 0.800623 | 1.321324e-48 | 6.181376e-46 |
| MsG0280011446.01 | MsG0380017879.01 | 0.816578 | 5.143685e-52 | 3.658755e-49 |
| MsG0380016581.01 | MsG0380017879.01 | 0.821376 | 4.172896e-53 | 3.388950e-50 |
| MsG0380016624.01 | MsG0380017879.01 | 0.827436 | 1.566261e-54 | 1.512039e-51 |
| MsG0380017403.01 | MsG0380017879.01 | 0.811342 | 7.343434e-51 | 4.533892e-48 |
| MsG0380017879.01 | MsG0480020886.01 | 0.810538 | 1.096604e-50 | 6.627668e-48 |
| MsG0380017879.01 | MsG0480023224.01 | 0.802762 | 4.810179e-49 | 2.375866e-46 |
| MsG0380017879.01 | MsG0480023245.01 | 0.804694 | 1.910084e-49 | 9.913893e-47 |
| MsG0380017879.01 | MsG0580024306.01 | 0.812031 | 5.202553e-51 | 3.271419e-48 |
| MsG0380017879.01 | MsG0580026596.01 | 0.802421 | 5.655451e-49 | 2.769263e-46 |
| MsG0380017879.01 | MsG0580027942.01 | 0.803217 | 3.872789e-49 | 1.935145e-46 |
| MsG0380017879.01 | MsG0580028969.01 | 0.808543 | 2.941189e-50 | 1.686321e-47 |
| MsG0380017879.01 | MsG0780035958.01 | 0.802152 | 6.425267e-49 | 3.124559e-46 |
| MsG0380017879.01 | MsG0780040505.01 | 0.810734 | 9.946212e-51 | 6.043207e-48 |
| MsG0380017879.01 | MsG0880046244.01 | 0.823658 | 1.229997e-53 | 1.065782e-50 |
| MsG0380017879.01 | MsG0880046329.01 | 0.812868 | 3.413883e-51 | 2.195167e-48 |
| MsG0380017879.01 | MsG0880047019.01 | 0.800553 | 1.365314e-48 | 6.375835e-46 |
| MsG0280007815.01 | MsG0380017879.01 | 0.814503 | 1.490652e-51 | 1.002037e-48 |
| MsG0280009024.01 | MsG0380017879.01 | 0.821380 | 4.163810e-53 | 3.381931e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017879.01.T05 | MTR_3g114460 | 92.381 | 315 | 23 | 1 | 1 | 315 | 64 | 377 | 0.0 | 566 |
| MsG0380017879.01.T05 | MTR_8g068520 | 52.903 | 310 | 127 | 7 | 9 | 314 | 83 | 377 | 1.66e-101 | 303 |
| MsG0380017879.01.T05 | MTR_1g025960 | 51.562 | 320 | 125 | 7 | 6 | 314 | 66 | 366 | 3.93e-98 | 294 |
| MsG0380017879.01.T05 | MTR_1g100200 | 26.452 | 310 | 176 | 7 | 16 | 311 | 66 | 337 | 1.30e-20 | 90.9 |
| MsG0380017879.01.T05 | MTR_1g100200 | 26.452 | 310 | 176 | 7 | 16 | 311 | 69 | 340 | 1.53e-20 | 90.9 |
| MsG0380017879.01.T05 | MTR_1g100200 | 26.452 | 310 | 176 | 7 | 16 | 311 | 89 | 360 | 1.99e-20 | 90.9 |
| MsG0380017879.01.T06 | MTR_3g114460 | 93.080 | 289 | 19 | 1 | 1 | 289 | 90 | 377 | 0.0 | 543 |
| MsG0380017879.01.T06 | MTR_8g068520 | 52.740 | 292 | 119 | 7 | 1 | 288 | 101 | 377 | 5.85e-94 | 283 |
| MsG0380017879.01.T06 | MTR_1g025960 | 51.890 | 291 | 118 | 6 | 1 | 288 | 95 | 366 | 9.81e-90 | 271 |
| MsG0380017879.01.T06 | MTR_1g100200 | 26.224 | 286 | 171 | 6 | 6 | 285 | 86 | 337 | 3.30e-18 | 83.6 |
| MsG0380017879.01.T06 | MTR_1g100200 | 26.224 | 286 | 171 | 6 | 6 | 285 | 89 | 340 | 3.78e-18 | 83.6 |
| MsG0380017879.01.T06 | MTR_1g100200 | 26.224 | 286 | 171 | 6 | 6 | 285 | 109 | 360 | 5.13e-18 | 83.6 |
| MsG0380017879.01.T01 | MTR_3g114460 | 90.885 | 373 | 33 | 1 | 5 | 377 | 6 | 377 | 0.0 | 660 |
| MsG0380017879.01.T01 | MTR_8g068520 | 51.058 | 378 | 157 | 9 | 12 | 376 | 15 | 377 | 3.96e-117 | 345 |
| MsG0380017879.01.T01 | MTR_1g025960 | 50.394 | 381 | 155 | 9 | 7 | 376 | 9 | 366 | 3.57e-112 | 332 |
| MsG0380017879.01.T01 | MTR_1g100200 | 27.322 | 366 | 205 | 9 | 31 | 373 | 10 | 337 | 1.35e-26 | 108 |
| MsG0380017879.01.T01 | MTR_1g100200 | 27.397 | 365 | 204 | 9 | 32 | 373 | 14 | 340 | 2.70e-26 | 108 |
| MsG0380017879.01.T01 | MTR_1g100200 | 27.397 | 365 | 204 | 9 | 32 | 373 | 34 | 360 | 4.09e-26 | 108 |
| MsG0380017879.01.T03 | MTR_3g114460 | 87.302 | 378 | 30 | 2 | 1 | 361 | 1 | 377 | 0.0 | 632 |
| MsG0380017879.01.T03 | MTR_8g068520 | 50.815 | 368 | 156 | 8 | 13 | 360 | 15 | 377 | 1.01e-114 | 338 |
| MsG0380017879.01.T03 | MTR_1g025960 | 50.549 | 364 | 152 | 7 | 15 | 360 | 13 | 366 | 2.68e-110 | 327 |
| MsG0380017879.01.T03 | MTR_1g100200 | 28.571 | 350 | 204 | 9 | 32 | 357 | 10 | 337 | 5.61e-29 | 115 |
| MsG0380017879.01.T03 | MTR_1g100200 | 28.653 | 349 | 203 | 9 | 33 | 357 | 14 | 340 | 8.34e-29 | 114 |
| MsG0380017879.01.T03 | MTR_1g100200 | 28.653 | 349 | 203 | 9 | 33 | 357 | 34 | 360 | 1.33e-28 | 114 |
| MsG0380017879.01.T02 | MTR_3g114460 | 88.889 | 378 | 28 | 2 | 1 | 365 | 1 | 377 | 0.0 | 646 |
| MsG0380017879.01.T02 | MTR_8g068520 | 50.949 | 369 | 158 | 8 | 13 | 364 | 15 | 377 | 8.23e-116 | 341 |
| MsG0380017879.01.T02 | MTR_1g025960 | 50.685 | 365 | 154 | 7 | 15 | 364 | 13 | 366 | 5.85e-111 | 328 |
| MsG0380017879.01.T02 | MTR_1g100200 | 26.912 | 353 | 210 | 8 | 32 | 361 | 10 | 337 | 4.51e-28 | 112 |
| MsG0380017879.01.T02 | MTR_1g100200 | 26.989 | 352 | 209 | 8 | 33 | 361 | 14 | 340 | 6.23e-28 | 112 |
| MsG0380017879.01.T02 | MTR_1g100200 | 26.989 | 352 | 209 | 8 | 33 | 361 | 34 | 360 | 9.15e-28 | 112 |
| MsG0380017879.01.T04 | MTR_3g114460 | 91.799 | 378 | 30 | 1 | 1 | 378 | 1 | 377 | 0.0 | 678 |
| MsG0380017879.01.T04 | MTR_8g068520 | 51.058 | 378 | 157 | 9 | 13 | 377 | 15 | 377 | 6.41e-117 | 345 |
| MsG0380017879.01.T04 | MTR_1g025960 | 50.802 | 374 | 153 | 8 | 15 | 377 | 13 | 366 | 3.14e-112 | 332 |
| MsG0380017879.01.T04 | MTR_1g100200 | 27.322 | 366 | 205 | 9 | 32 | 374 | 10 | 337 | 1.34e-26 | 108 |
| MsG0380017879.01.T04 | MTR_1g100200 | 27.397 | 365 | 204 | 9 | 33 | 374 | 14 | 340 | 1.95e-26 | 108 |
| MsG0380017879.01.T04 | MTR_1g100200 | 27.397 | 365 | 204 | 9 | 33 | 374 | 34 | 360 | 3.41e-26 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017879.01.T05 | AT1G20180 | 39.803 | 304 | 172 | 5 | 16 | 314 | 91 | 388 | 1.32e-62 | 203 |
| MsG0380017879.01.T05 | AT1G20180 | 36.093 | 302 | 151 | 6 | 16 | 312 | 91 | 355 | 3.72e-51 | 173 |
| MsG0380017879.01.T05 | AT3G49070 | 31.023 | 303 | 174 | 6 | 15 | 313 | 88 | 359 | 4.98e-32 | 123 |
| MsG0380017879.01.T05 | AT3G49070 | 31.023 | 303 | 174 | 6 | 15 | 313 | 128 | 399 | 9.43e-32 | 123 |
| MsG0380017879.01.T06 | AT1G20180 | 40.702 | 285 | 158 | 5 | 9 | 288 | 110 | 388 | 2.18e-59 | 194 |
| MsG0380017879.01.T06 | AT1G20180 | 36.749 | 283 | 137 | 6 | 9 | 286 | 110 | 355 | 7.16e-48 | 163 |
| MsG0380017879.01.T06 | AT3G49070 | 32.014 | 278 | 158 | 5 | 10 | 287 | 113 | 359 | 9.31e-31 | 119 |
| MsG0380017879.01.T06 | AT3G49070 | 32.014 | 278 | 158 | 5 | 10 | 287 | 153 | 399 | 1.28e-30 | 119 |
| MsG0380017879.01.T01 | AT1G20180 | 39.617 | 366 | 200 | 7 | 26 | 376 | 29 | 388 | 1.37e-68 | 221 |
| MsG0380017879.01.T01 | AT1G20180 | 36.538 | 364 | 179 | 8 | 26 | 374 | 29 | 355 | 2.37e-57 | 191 |
| MsG0380017879.01.T01 | AT3G49070 | 29.134 | 381 | 217 | 8 | 12 | 375 | 15 | 359 | 4.49e-33 | 127 |
| MsG0380017879.01.T01 | AT3G49070 | 29.024 | 379 | 216 | 8 | 14 | 375 | 57 | 399 | 3.26e-32 | 125 |
| MsG0380017879.01.T01 | AT3G19330 | 26.203 | 374 | 240 | 13 | 11 | 373 | 24 | 372 | 8.74e-12 | 66.6 |
| MsG0380017879.01.T01 | AT3G19330 | 26.203 | 374 | 240 | 13 | 11 | 373 | 24 | 372 | 8.74e-12 | 66.6 |
| MsG0380017879.01.T01 | AT3G19250 | 22.310 | 381 | 262 | 11 | 1 | 373 | 1 | 355 | 4.71e-11 | 63.9 |
| MsG0380017879.01.T03 | AT1G20180 | 39.669 | 363 | 183 | 8 | 27 | 358 | 29 | 386 | 7.87e-66 | 213 |
| MsG0380017879.01.T03 | AT1G20180 | 36.364 | 363 | 164 | 9 | 27 | 358 | 29 | 355 | 1.00e-54 | 184 |
| MsG0380017879.01.T03 | AT3G49070 | 29.863 | 365 | 214 | 8 | 15 | 359 | 17 | 359 | 4.23e-33 | 127 |
| MsG0380017879.01.T03 | AT3G49070 | 29.863 | 365 | 214 | 8 | 15 | 359 | 57 | 399 | 6.69e-33 | 127 |
| MsG0380017879.01.T03 | AT3G19330 | 26.486 | 370 | 219 | 14 | 16 | 357 | 28 | 372 | 7.19e-12 | 66.6 |
| MsG0380017879.01.T03 | AT3G19330 | 26.486 | 370 | 219 | 14 | 16 | 357 | 28 | 372 | 7.19e-12 | 66.6 |
| MsG0380017879.01.T02 | AT1G20180 | 39.560 | 364 | 186 | 8 | 27 | 362 | 29 | 386 | 2.41e-66 | 215 |
| MsG0380017879.01.T02 | AT1G20180 | 36.264 | 364 | 167 | 9 | 27 | 362 | 29 | 355 | 3.45e-55 | 185 |
| MsG0380017879.01.T02 | AT3G49070 | 29.973 | 367 | 215 | 9 | 15 | 363 | 17 | 359 | 1.15e-32 | 125 |
| MsG0380017879.01.T02 | AT3G49070 | 29.973 | 367 | 215 | 9 | 15 | 363 | 57 | 399 | 2.42e-32 | 125 |
| MsG0380017879.01.T02 | AT3G19330 | 26.027 | 365 | 231 | 13 | 16 | 361 | 28 | 372 | 9.90e-12 | 66.2 |
| MsG0380017879.01.T02 | AT3G19330 | 26.027 | 365 | 231 | 13 | 16 | 361 | 28 | 372 | 9.90e-12 | 66.2 |
| MsG0380017879.01.T04 | AT1G20180 | 39.617 | 366 | 200 | 7 | 27 | 377 | 29 | 388 | 2.33e-68 | 221 |
| MsG0380017879.01.T04 | AT1G20180 | 36.538 | 364 | 179 | 8 | 27 | 375 | 29 | 355 | 3.57e-57 | 191 |
| MsG0380017879.01.T04 | AT3G49070 | 29.024 | 379 | 216 | 8 | 15 | 376 | 17 | 359 | 1.77e-32 | 125 |
| MsG0380017879.01.T04 | AT3G49070 | 29.024 | 379 | 216 | 8 | 15 | 376 | 57 | 399 | 3.29e-32 | 125 |
| MsG0380017879.01.T04 | AT3G19330 | 26.216 | 370 | 237 | 13 | 16 | 374 | 28 | 372 | 1.28e-11 | 65.9 |
| MsG0380017879.01.T04 | AT3G19330 | 26.216 | 370 | 237 | 13 | 16 | 374 | 28 | 372 | 1.28e-11 | 65.9 |
Find 56 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTGCCAAACTAACTTTA+TGG | 0.110914 | 3:+101795218 | MsG0380017879.01.T01:CDS |
| TTTGCTTTCCATAGCATAAT+TGG | 0.142059 | 3:+101794904 | MsG0380017879.01.T01:CDS |
| GCGGAAGTTAATAACATTAT+TGG | 0.198214 | 3:-101794728 | None:intergenic |
| CGTCAAACTAGATTTGATTA+TGG | 0.245353 | 3:+101794565 | MsG0380017879.01.T01:CDS |
| AAGAGATCAAAGTGGGAAAA+TGG | 0.266547 | 3:+101794979 | MsG0380017879.01.T01:CDS |
| TTATATAGTGATAAATGATT+TGG | 0.292938 | 3:+101795059 | MsG0380017879.01.T01:CDS |
| GATATTTGTGTGAAGAATTA+TGG | 0.298600 | 3:+101795144 | MsG0380017879.01.T01:CDS |
| TGTAACAAAGCTCAAGGATA+TGG | 0.306198 | 3:+101794352 | MsG0380017879.01.T01:CDS |
| TGTCATATTCAACGACTCTT+TGG | 0.315226 | 3:-101794618 | None:intergenic |
| ACTGATCCATAAAGTTAGTT+TGG | 0.327126 | 3:-101795224 | None:intergenic |
| TATGTAGTTGGTGGATCCTT+TGG | 0.338019 | 3:+101794943 | MsG0380017879.01.T01:CDS |
| TCTTTCTTCCGAAGAAAATC+TGG | 0.338811 | 3:+101794014 | MsG0380017879.01.T01:CDS |
| AGAAGAAGAAGAAGTGGAAC+AGG | 0.349044 | 3:-101794413 | None:intergenic |
| CGTTTCAGAAACCGCCCTTT+TGG | 0.386650 | 3:+101794867 | MsG0380017879.01.T01:CDS |
| CAAGTTGATGTTGCTACAAA+AGG | 0.387502 | 3:+101795033 | MsG0380017879.01.T01:CDS |
| TGCAAGAAAGTTGGAGGAAT+CGG | 0.391183 | 3:+101794838 | MsG0380017879.01.T01:CDS |
| GGTTAAGAGGTTAGAGGATG+AGG | 0.399784 | 3:+101795098 | MsG0380017879.01.T01:CDS |
| GCTGCTGCCCCTTATGTAGT+TGG | 0.407925 | 3:+101794931 | MsG0380017879.01.T01:CDS |
| TTTGTAACAATTTCTTGTCT+AGG | 0.414371 | 3:-101794445 | None:intergenic |
| TTGCTTTCCATAGCATAATT+GGG | 0.423917 | 3:+101794905 | MsG0380017879.01.T01:CDS |
| AAGAGGTTATGCAAGAAAGT+TGG | 0.425159 | 3:+101794829 | MsG0380017879.01.T01:CDS |
| AAGGATCCACCAACTACATA+AGG | 0.429019 | 3:-101794940 | None:intergenic |
| TGCTGCGAGAAAATCCTTCA+AGG | 0.432150 | 3:+101794538 | MsG0380017879.01.T01:CDS |
| AAGAATTATGGTCATGGTAA+TGG | 0.433359 | 3:+101795156 | MsG0380017879.01.T01:CDS |
| TAGTTTGACGAACACCTTGA+AGG | 0.455617 | 3:-101794552 | None:intergenic |
| CCGTATGGTTAAGAGGTTAG+AGG | 0.460969 | 3:+101795092 | MsG0380017879.01.T01:CDS |
| AGACTAACAAAGCCACCAAA+AGG | 0.461061 | 3:-101794882 | None:intergenic |
| AACTAACTTTATGGATCAGT+TGG | 0.461239 | 3:+101795227 | MsG0380017879.01.T01:CDS |
| AAGTTGATGTTGCTACAAAA+GGG | 0.472572 | 3:+101795034 | MsG0380017879.01.T01:CDS |
| CTATGAGCCGTATGGTTAAG+AGG | 0.474901 | 3:+101795085 | MsG0380017879.01.T01:CDS |
| AGGATCCACCAACTACATAA+GGG | 0.488584 | 3:-101794939 | None:intergenic |
| GCAAAGACATGCTTCTAAGC+TGG | 0.506421 | 3:-101794518 | None:intergenic |
| TAGAGGATGAGGTTGAACAT+TGG | 0.517451 | 3:+101795109 | MsG0380017879.01.T01:CDS |
| GAAGAAGAAGAGATCAAAGT+GGG | 0.525471 | 3:+101794972 | MsG0380017879.01.T01:CDS |
| TGAGGTTGAACATTGGAGAG+AGG | 0.530933 | 3:+101795116 | MsG0380017879.01.T01:CDS |
| AGGTTATGCAAGAAAGTTGG+AGG | 0.533933 | 3:+101794832 | MsG0380017879.01.T01:CDS |
| GACTAACAAAGCCACCAAAA+GGG | 0.538003 | 3:-101794881 | None:intergenic |
| CTTCTTCTTCATCAAACCAA+AGG | 0.546523 | 3:-101794959 | None:intergenic |
| CAGCTAACCCAATTATGCTA+TGG | 0.556147 | 3:-101794912 | None:intergenic |
| GGATCAGTTGGAAGAGCTTG+AGG | 0.556162 | 3:+101795239 | MsG0380017879.01.T01:CDS |
| GCTGCCCCTTATGTAGTTGG+TGG | 0.559630 | 3:+101794934 | MsG0380017879.01.T01:CDS |
| TAAGGTGCATCATCTTCTAG+TGG | 0.565366 | 3:+101794483 | MsG0380017879.01.T01:CDS |
| GACATATGTAACAAAGCTCA+AGG | 0.571855 | 3:+101794346 | MsG0380017879.01.T01:CDS |
| CCTCTAACCTCTTAACCATA+CGG | 0.573081 | 3:-101795092 | None:intergenic |
| AATTTGTTCTGAATGCATTG+AGG | 0.584357 | 3:-101794315 | None:intergenic |
| AGTTGATGTTGCTACAAAAG+GGG | 0.592162 | 3:+101795035 | MsG0380017879.01.T01:CDS |
| TGAAGAAGAAGAGATCAAAG+TGG | 0.592386 | 3:+101794971 | MsG0380017879.01.T01:CDS |
| TTTGGATACTATGAGCCGTA+TGG | 0.601069 | 3:+101795077 | MsG0380017879.01.T01:CDS |
| ATTATGGTCATGGTAATGGA+AGG | 0.610111 | 3:+101795160 | MsG0380017879.01.T01:CDS |
| AATATTGAAGATGGTTCTGA+GGG | 0.614569 | 3:+101795188 | MsG0380017879.01.T01:CDS |
| AAGGTGTGTAATATTGAAGA+TGG | 0.621343 | 3:+101795179 | MsG0380017879.01.T01:CDS |
| ATTATCGAAAGAACGTGATG+TGG | 0.633657 | 3:+101794252 | MsG0380017879.01.T01:CDS |
| GGATCCACCAACTACATAAG+GGG | 0.639730 | 3:-101794938 | None:intergenic |
| TAATATTGAAGATGGTTCTG+AGG | 0.641414 | 3:+101795187 | MsG0380017879.01.T01:CDS |
| TGTGTGAAGAATTATGGTCA+TGG | 0.650409 | 3:+101795150 | MsG0380017879.01.T01:CDS |
| TAACAAAGCCACCAAAAGGG+CGG | 0.696209 | 3:-101794878 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTAAATTAAAAATAAA+CGG | - | Chr3:101794079-101794098 | None:intergenic | 0.0% |
| !! | TAACTAATTAATTAATTACT+TGG | - | Chr3:101794164-101794183 | None:intergenic | 10.0% |
| !! | TTATATAGTGATAAATGATT+TGG | + | Chr3:101795059-101795078 | MsG0380017879.01.T01:CDS | 15.0% |
| !! | GAAATAATTAAAAAACATGC+AGG | + | Chr3:101794223-101794242 | MsG0380017879.01.T01:intron | 20.0% |
| !! | GAAGAAAAATAACAATAAAG+AGG | + | Chr3:101794812-101794831 | MsG0380017879.01.T01:CDS | 20.0% |
| ! | AAAAATGATCAAGTGCTTTA+AGG | + | Chr3:101794465-101794484 | MsG0380017879.01.T01:CDS | 25.0% |
| ! | ATAATTAAAAAACATGCAGG+TGG | + | Chr3:101794226-101794245 | MsG0380017879.01.T01:intron | 25.0% |
| ! | GATATTTGTGTGAAGAATTA+TGG | + | Chr3:101795144-101795163 | MsG0380017879.01.T01:CDS | 25.0% |
| ! | TTTGTAACAATTTCTTGTCT+AGG | - | Chr3:101794448-101794467 | None:intergenic | 25.0% |
| AACTAACTTTATGGATCAGT+TGG | + | Chr3:101795227-101795246 | MsG0380017879.01.T01:CDS | 30.0% | |
| AAGAATTATGGTCATGGTAA+TGG | + | Chr3:101795156-101795175 | MsG0380017879.01.T01:CDS | 30.0% | |
| AAGGTGTGTAATATTGAAGA+TGG | + | Chr3:101795179-101795198 | MsG0380017879.01.T01:CDS | 30.0% | |
| ACTGATCCATAAAGTTAGTT+TGG | - | Chr3:101795227-101795246 | None:intergenic | 30.0% | |
| CGTCAAACTAGATTTGATTA+TGG | + | Chr3:101794565-101794584 | MsG0380017879.01.T01:CDS | 30.0% | |
| GCGGAAGTTAATAACATTAT+TGG | - | Chr3:101794731-101794750 | None:intergenic | 30.0% | |
| TGATTGCCAAACTAACTTTA+TGG | + | Chr3:101795218-101795237 | MsG0380017879.01.T01:CDS | 30.0% | |
| TTATGTTTGAATTGAACTCC+AGG | + | Chr3:101794124-101794143 | MsG0380017879.01.T01:intron | 30.0% | |
| TTGCTTTCCATAGCATAATT+GGG | + | Chr3:101794905-101794924 | MsG0380017879.01.T01:CDS | 30.0% | |
| TTTGCTTTCCATAGCATAAT+TGG | + | Chr3:101794904-101794923 | MsG0380017879.01.T01:CDS | 30.0% | |
| ! | AATTTGTTCTGAATGCATTG+AGG | - | Chr3:101794318-101794337 | None:intergenic | 30.0% |
| ! | TAATATTGAAGATGGTTCTG+AGG | + | Chr3:101795187-101795206 | MsG0380017879.01.T01:CDS | 30.0% |
| ! | TGTGTTTTTCTTGAATATCG+CGG | - | Chr3:101794750-101794769 | None:intergenic | 30.0% |
| ! | TTAACTCACCAGATTTTCTT+CGG | - | Chr3:101794025-101794044 | None:intergenic | 30.0% |
| !! | AAGTTGATGTTGCTACAAAA+GGG | + | Chr3:101795034-101795053 | MsG0380017879.01.T01:CDS | 30.0% |
| !! | AATATTGAAGATGGTTCTGA+GGG | + | Chr3:101795188-101795207 | MsG0380017879.01.T01:CDS | 30.0% |
| AAGAGATCAAAGTGGGAAAA+TGG | + | Chr3:101794979-101794998 | MsG0380017879.01.T01:CDS | 35.0% | |
| AAGAGGTTATGCAAGAAAGT+TGG | + | Chr3:101794829-101794848 | MsG0380017879.01.T01:CDS | 35.0% | |
| ATTAAAAAACATGCAGGTGG+TGG | + | Chr3:101794229-101794248 | MsG0380017879.01.T01:intron | 35.0% | |
| ATTATCGAAAGAACGTGATG+TGG | + | Chr3:101794252-101794271 | MsG0380017879.01.T01:CDS | 35.0% | |
| CTTCTTCTTCATCAAACCAA+AGG | - | Chr3:101794962-101794981 | None:intergenic | 35.0% | |
| GAAGAAGAAGAGATCAAAGT+GGG | + | Chr3:101794972-101794991 | MsG0380017879.01.T01:CDS | 35.0% | |
| GACATATGTAACAAAGCTCA+AGG | + | Chr3:101794346-101794365 | MsG0380017879.01.T01:CDS | 35.0% | |
| TAGCAAAGAAGAAGAAGAAG+TGG | - | Chr3:101794422-101794441 | None:intergenic | 35.0% | |
| TCTTTCTTCCGAAGAAAATC+TGG | + | Chr3:101794014-101794033 | MsG0380017879.01.T01:CDS | 35.0% | |
| TGAAGAAGAAGAGATCAAAG+TGG | + | Chr3:101794971-101794990 | MsG0380017879.01.T01:CDS | 35.0% | |
| TGTAACAAAGCTCAAGGATA+TGG | + | Chr3:101794352-101794371 | MsG0380017879.01.T01:CDS | 35.0% | |
| TGTCATATTCAACGACTCTT+TGG | - | Chr3:101794621-101794640 | None:intergenic | 35.0% | |
| TGTGTGAAGAATTATGGTCA+TGG | + | Chr3:101795150-101795169 | MsG0380017879.01.T01:CDS | 35.0% | |
| ! | AGTTGATGTTGCTACAAAAG+GGG | + | Chr3:101795035-101795054 | MsG0380017879.01.T01:CDS | 35.0% |
| !! | ATTATGGTCATGGTAATGGA+AGG | + | Chr3:101795160-101795179 | MsG0380017879.01.T01:CDS | 35.0% |
| !! | CAAGTTGATGTTGCTACAAA+AGG | + | Chr3:101795033-101795052 | MsG0380017879.01.T01:CDS | 35.0% |
| AAGGATCCACCAACTACATA+AGG | - | Chr3:101794943-101794962 | None:intergenic | 40.0% | |
| AGAAGAAGAAGAAGTGGAAC+AGG | - | Chr3:101794416-101794435 | None:intergenic | 40.0% | |
| AGACTAACAAAGCCACCAAA+AGG | - | Chr3:101794885-101794904 | None:intergenic | 40.0% | |
| AGGATCCACCAACTACATAA+GGG | - | Chr3:101794942-101794961 | None:intergenic | 40.0% | |
| AGGTTATGCAAGAAAGTTGG+AGG | + | Chr3:101794832-101794851 | MsG0380017879.01.T01:CDS | 40.0% | |
| CAGCTAACCCAATTATGCTA+TGG | - | Chr3:101794915-101794934 | None:intergenic | 40.0% | |
| CCTCTAACCTCTTAACCATA+CGG | - | Chr3:101795095-101795114 | None:intergenic | 40.0% | |
| GACTAACAAAGCCACCAAAA+GGG | - | Chr3:101794884-101794903 | None:intergenic | 40.0% | |
| TAAGGTGCATCATCTTCTAG+TGG | + | Chr3:101794483-101794502 | MsG0380017879.01.T01:CDS | 40.0% | |
| TAGAGGATGAGGTTGAACAT+TGG | + | Chr3:101795109-101795128 | MsG0380017879.01.T01:CDS | 40.0% | |
| TAGTTTGACGAACACCTTGA+AGG | - | Chr3:101794555-101794574 | None:intergenic | 40.0% | |
| TGCAAGAAAGTTGGAGGAAT+CGG | + | Chr3:101794838-101794857 | MsG0380017879.01.T01:CDS | 40.0% | |
| TGTTTGAATTGAACTCCAGG+AGG | + | Chr3:101794127-101794146 | MsG0380017879.01.T01:intron | 40.0% | |
| TTTGGATACTATGAGCCGTA+TGG | + | Chr3:101795077-101795096 | MsG0380017879.01.T01:CDS | 40.0% | |
| !! | TATGTAGTTGGTGGATCCTT+TGG | + | Chr3:101794943-101794962 | MsG0380017879.01.T01:CDS | 40.0% |
| CCGTATGGTTAAGAGGTTAG+AGG | + | Chr3:101795092-101795111 | MsG0380017879.01.T01:CDS | 45.0% | |
| CTATGAGCCGTATGGTTAAG+AGG | + | Chr3:101795085-101795104 | MsG0380017879.01.T01:CDS | 45.0% | |
| GCAAAGACATGCTTCTAAGC+TGG | - | Chr3:101794521-101794540 | None:intergenic | 45.0% | |
| GGATCCACCAACTACATAAG+GGG | - | Chr3:101794941-101794960 | None:intergenic | 45.0% | |
| GGTTAAGAGGTTAGAGGATG+AGG | + | Chr3:101795098-101795117 | MsG0380017879.01.T01:CDS | 45.0% | |
| TAACAAAGCCACCAAAAGGG+CGG | - | Chr3:101794881-101794900 | None:intergenic | 45.0% | |
| TGAGGTTGAACATTGGAGAG+AGG | + | Chr3:101795116-101795135 | MsG0380017879.01.T01:CDS | 45.0% | |
| TGCTGCGAGAAAATCCTTCA+AGG | + | Chr3:101794538-101794557 | MsG0380017879.01.T01:CDS | 45.0% | |
| GGATCAGTTGGAAGAGCTTG+AGG | + | Chr3:101795239-101795258 | MsG0380017879.01.T01:CDS | 50.0% | |
| ! | CGTTTCAGAAACCGCCCTTT+TGG | + | Chr3:101794867-101794886 | MsG0380017879.01.T01:CDS | 50.0% |
| ! | TTCAGAAACCGCCCTTTTGG+TGG | + | Chr3:101794870-101794889 | MsG0380017879.01.T01:CDS | 50.0% |
| GCTGCCCCTTATGTAGTTGG+TGG | + | Chr3:101794934-101794953 | MsG0380017879.01.T01:CDS | 55.0% | |
| GCTGCTGCCCCTTATGTAGT+TGG | + | Chr3:101794931-101794950 | MsG0380017879.01.T01:CDS | 55.0% | |
| ! | TGAACTCCAGGAGGTGTGCT+TGG | + | Chr3:101794136-101794155 | MsG0380017879.01.T01:intron | 55.0% |
| TTGGCTCCAAGCACACCTCC+TGG | - | Chr3:101794145-101794164 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 101793999 | 101795341 | 101793999 | ID=MsG0380017879.01;Name=MsG0380017879.01 |
| Chr3 | mRNA | 101793999 | 101795341 | 101793999 | ID=MsG0380017879.01.T01;Parent=MsG0380017879.01;Name=MsG0380017879.01.T01;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|0|0|2|0|378 |
| Chr3 | exon | 101793999 | 101794035 | 101793999 | ID=MsG0380017879.01.T01:exon:27429;Parent=MsG0380017879.01.T01 |
| Chr3 | exon | 101794242 | 101795341 | 101794242 | ID=MsG0380017879.01.T01:exon:27430;Parent=MsG0380017879.01.T01 |
| Chr3 | CDS | 101793999 | 101794035 | 101793999 | ID=MsG0380017879.01.T01:cds;Parent=MsG0380017879.01.T01 |
| Chr3 | CDS | 101794242 | 101795341 | 101794242 | ID=MsG0380017879.01.T01:cds;Parent=MsG0380017879.01.T01 |
| Chr3 | mRNA | 101794202 | 101795341 | 101794202 | ID=MsG0380017879.01.T02;Parent=MsG0380017879.01;Name=MsG0380017879.01.T02;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|0|0|2|0|366 |
| Chr3 | exon | 101794202 | 101794649 | 101794202 | ID=MsG0380017879.01.T02:exon:27431;Parent=MsG0380017879.01.T02 |
| Chr3 | exon | 101794689 | 101795341 | 101794689 | ID=MsG0380017879.01.T02:exon:27432;Parent=MsG0380017879.01.T02 |
| Chr3 | CDS | 101794202 | 101794649 | 101794202 | ID=MsG0380017879.01.T02:cds;Parent=MsG0380017879.01.T02 |
| Chr3 | CDS | 101794689 | 101795341 | 101794689 | ID=MsG0380017879.01.T02:cds;Parent=MsG0380017879.01.T02 |
| Chr3 | mRNA | 101794202 | 101795341 | 101794202 | ID=MsG0380017879.01.T03;Parent=MsG0380017879.01;Name=MsG0380017879.01.T03;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|0|0|2|0|362 |
| Chr3 | exon | 101794202 | 101794612 | 101794202 | ID=MsG0380017879.01.T03:exon:27433;Parent=MsG0380017879.01.T03 |
| Chr3 | exon | 101794664 | 101795341 | 101794664 | ID=MsG0380017879.01.T03:exon:27434;Parent=MsG0380017879.01.T03 |
| Chr3 | CDS | 101794202 | 101794612 | 101794202 | ID=MsG0380017879.01.T03:cds;Parent=MsG0380017879.01.T03 |
| Chr3 | CDS | 101794664 | 101795341 | 101794664 | ID=MsG0380017879.01.T03:cds;Parent=MsG0380017879.01.T03 |
| Chr3 | mRNA | 101794202 | 101795341 | 101794202 | ID=MsG0380017879.01.T04;Parent=MsG0380017879.01;Name=MsG0380017879.01.T04;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|379 |
| Chr3 | exon | 101794202 | 101795341 | 101794202 | ID=MsG0380017879.01.T04:exon:27435;Parent=MsG0380017879.01.T04 |
| Chr3 | CDS | 101794202 | 101795341 | 101794202 | ID=MsG0380017879.01.T04:cds;Parent=MsG0380017879.01.T04 |
| Chr3 | mRNA | 101794391 | 101795341 | 101794391 | ID=MsG0380017879.01.T05;Parent=MsG0380017879.01;Name=MsG0380017879.01.T05;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|0|1|0|316 |
| Chr3 | exon | 101794391 | 101795341 | 101794391 | ID=MsG0380017879.01.T05:exon:27436;Parent=MsG0380017879.01.T05 |
| Chr3 | CDS | 101794391 | 101795341 | 101794391 | ID=MsG0380017879.01.T05:cds;Parent=MsG0380017879.01.T05 |
| Chr3 | mRNA | 101794469 | 101795341 | 101794469 | ID=MsG0380017879.01.T06;Parent=MsG0380017879.01;Name=MsG0380017879.01.T06;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|0|1|0|290 |
| Chr3 | exon | 101794469 | 101795341 | 101794469 | ID=MsG0380017879.01.T06:exon:27437;Parent=MsG0380017879.01.T06 |
| Chr3 | CDS | 101794469 | 101795341 | 101794469 | ID=MsG0380017879.01.T06:cds;Parent=MsG0380017879.01.T06 |
| Gene Sequence |
| Protein sequence |
