AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016624.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016624.01.T01 MTR_3g092150 97.959 245 5 0 1 245 1 245 1.32e-180 495
MsG0380016624.01.T01 MTR_8g026960 43.269 208 110 4 42 245 38 241 2.50e-47 157
MsG0380016624.01.T01 MTR_6g007647 45.185 135 63 2 7 130 25 159 3.74e-30 114
MsG0380016624.01.T01 MTR_6g007647 57.447 94 40 0 37 130 32 125 4.91e-30 112
MsG0380016624.01.T01 MTR_4g107650 56.122 98 43 0 37 134 94 191 5.12e-29 112
MsG0380016624.01.T01 MTR_6g011610 56.383 94 41 0 37 130 95 188 8.32e-29 110
MsG0380016624.01.T01 MTR_6g011610 56.383 94 41 0 37 130 76 169 8.42e-29 109
MsG0380016624.01.T01 MTR_8g468210 54.255 94 43 0 37 130 76 169 4.43e-28 108
MsG0380016624.01.T01 MTR_1g061660 49.533 107 46 2 37 142 58 157 1.36e-26 103
MsG0380016624.01.T01 MTR_5g038280 55.435 92 41 0 39 130 88 179 1.97e-26 105
MsG0380016624.01.T01 MTR_8g089895 55.435 92 41 0 39 130 55 146 2.61e-26 104
MsG0380016624.01.T01 MTR_5g039000 54.255 94 43 0 37 130 76 169 3.12e-26 103
MsG0380016624.01.T01 MTR_3g086790 51.064 94 46 0 37 130 59 152 6.65e-26 103
MsG0380016624.01.T01 MTR_7g103340 41.304 138 67 3 37 160 62 199 4.17e-25 99.4
MsG0380016624.01.T01 MTR_8g469430 54.348 92 42 0 39 130 90 181 5.68e-25 100
MsG0380016624.01.T01 MTR_3g080100 49.474 95 48 0 36 130 18 112 6.45e-25 98.6
MsG0380016624.01.T01 MTR_3g080100 49.474 95 48 0 36 130 57 151 1.06e-24 99.0
MsG0380016624.01.T01 MTR_7g010020 47.826 92 48 0 39 130 81 172 3.22e-21 90.1
MsG0380016624.01.T01 MTR_5g019680 47.368 114 46 2 31 130 39 152 5.20e-21 87.8
MsG0380016624.01.T01 MTR_5g019650 52.577 97 38 2 31 120 39 134 2.05e-19 84.0
MsG0380016624.01.T01 MTR_4g100550 44.211 95 46 1 39 133 121 208 1.01e-13 69.3
MsG0380016624.01.T01 MTR_7g093430 41.346 104 48 2 33 133 155 248 1.05e-12 66.6
MsG0380016624.01.T01 MTR_4g126900 42.857 84 48 0 41 124 91 174 1.93e-12 65.1
MsG0380016624.01.T01 MTR_4g097600 43.158 95 47 1 39 133 128 215 2.99e-12 65.1
MsG0380016624.01.T01 MTR_5g014890 43.750 96 53 1 39 133 124 219 5.47e-12 64.3
MsG0380016624.01.T01 MTR_1g017090 43.158 95 47 1 39 133 131 218 8.61e-12 63.9
MsG0380016624.01.T01 MTR_1g054285 39.423 104 56 1 33 136 149 245 1.53e-11 63.2
MsG0380016624.01.T01 MTR_3g103590 45.333 75 41 0 36 110 143 217 1.72e-11 63.2
MsG0380016624.01.T01 MTR_8g006705 42.105 95 48 1 39 133 147 234 2.13e-11 63.2
MsG0380016624.01.T01 MTR_7g027265 31.250 144 73 4 35 177 17 135 2.45e-11 60.5
MsG0380016624.01.T01 MTR_2g061030 40.000 95 50 1 39 133 201 288 8.08e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016624.01.T01 AT2G46680 41.909 241 119 5 25 245 19 258 1.17e-44 150
MsG0380016624.01.T01 AT2G46680 42.259 239 119 5 25 245 19 256 1.48e-43 148
MsG0380016624.01.T01 AT3G61890 68.132 91 29 0 41 131 33 123 2.17e-38 134
MsG0380016624.01.T01 AT3G01470 40.972 144 85 0 37 180 67 210 7.46e-30 112
MsG0380016624.01.T01 AT2G22430 56.383 94 41 0 37 130 61 154 4.75e-29 111
MsG0380016624.01.T01 AT4G40060 52.041 98 47 0 37 134 58 155 2.21e-28 109
MsG0380016624.01.T01 AT1G69780 54.255 94 43 0 37 130 84 177 9.64e-28 107
MsG0380016624.01.T01 AT5G15150 53.191 94 44 0 37 130 114 207 3.92e-26 103
MsG0380016624.01.T01 AT2G18550 50.943 106 45 1 39 137 62 167 7.46e-26 101
MsG0380016624.01.T01 AT5G65310 51.064 94 46 0 37 130 53 146 3.05e-25 101
MsG0380016624.01.T01 AT5G65310 51.064 94 46 0 37 130 71 164 3.99e-25 101
MsG0380016624.01.T01 AT4G36740 42.647 136 61 2 17 135 25 160 9.08e-25 98.2
MsG0380016624.01.T01 AT4G36740 50.962 104 44 1 39 135 56 159 1.20e-24 97.8
MsG0380016624.01.T01 AT5G66700 51.000 100 42 1 39 131 72 171 1.31e-24 98.2
MsG0380016624.01.T01 AT3G01220 48.936 94 48 0 37 130 86 179 7.98e-24 97.4
MsG0380016624.01.T01 AT5G03790 43.478 115 54 2 20 134 63 166 1.70e-23 95.1
MsG0380016624.01.T01 AT1G26960 42.969 128 65 2 11 130 36 163 1.85e-22 92.8
MsG0380016624.01.T01 AT5G03790 46.000 100 50 1 20 119 63 158 7.15e-22 90.1
MsG0380016624.01.T01 AT1G27045 40.299 134 74 2 9 138 35 166 4.54e-21 89.4
MsG0380016624.01.T01 AT1G27045 45.361 97 53 0 34 130 21 117 6.44e-20 84.7
MsG0380016624.01.T01 AT1G27045 45.361 97 53 0 34 130 21 117 6.44e-20 84.7
MsG0380016624.01.T01 AT1G27045 39.683 126 72 1 9 130 35 160 7.48e-20 85.5
MsG0380016624.01.T01 AT1G27045 45.361 97 53 0 34 130 13 109 8.34e-20 84.0
MsG0380016624.01.T01 AT2G36610 42.308 104 45 2 39 134 72 168 2.61e-19 82.8
MsG0380016624.01.T01 AT5G53980 39.604 101 57 1 29 129 6 102 1.38e-15 72.4
MsG0380016624.01.T01 AT2G44910 42.000 100 51 1 39 138 164 256 1.51e-12 66.6
MsG0380016624.01.T01 AT3G60390 42.000 100 51 1 39 138 163 255 3.88e-12 65.1
MsG0380016624.01.T01 AT2G22800 42.105 95 48 1 39 133 114 201 1.19e-11 63.5
MsG0380016624.01.T01 AT5G06710 41.837 98 50 1 33 130 185 275 1.43e-11 63.9
MsG0380016624.01.T01 AT4G16780 42.105 95 48 1 39 133 130 217 6.17e-11 61.6
MsG0380016624.01.T01 AT2G01430 42.105 95 48 1 39 133 140 227 8.26e-11 61.2
MsG0380016624.01.T01 AT4G37790 41.053 95 49 1 39 133 127 214 8.94e-11 60.8

Find 36 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCCTAGACAAGTTGCTATA+TGG 0.329458 3:-85830039 MsG0380016624.01.T01:CDS
GCAAGTTGCATCTTCTTCCT+TGG 0.339735 3:+85830079 None:intergenic
AGCACAGAAGATGGTGGTTC+AGG 0.365057 3:-85829566 MsG0380016624.01.T01:CDS
GGTTCAGAAGCTAAGTTATA+TGG 0.367405 3:-85829630 MsG0380016624.01.T01:CDS
GAAGATGGTGGTTCAGGAAA+TGG 0.372815 3:-85829560 MsG0380016624.01.T01:CDS
GAAATGGATACAACAATTGC+AGG 0.379643 3:-85829544 MsG0380016624.01.T01:CDS
ATGCAACTTGCAAGAGATCT+TGG 0.385707 3:-85830067 MsG0380016624.01.T01:CDS
TTCTAAGTTTCAGTGCCTTA+AGG 0.396105 3:-85829933 MsG0380016624.01.T01:CDS
AAGACTCTTTCTCTTCCTTA+AGG 0.407960 3:+85829918 None:intergenic
TGATGAACAGATAAGATCAT+TGG 0.455417 3:-85830134 MsG0380016624.01.T01:CDS
ATGCAATAGTTCTCAATGGT+TGG 0.475551 3:-85829306 MsG0380016624.01.T01:CDS
AATATTCATGAAGGGTTCAA+AGG 0.493550 3:-85830244 MsG0380016624.01.T01:CDS
GAAGAAGAGAATATTCATGA+AGG 0.503417 3:-85830253 MsG0380016624.01.T01:CDS
CAGAATCAAAGTTGGAGCCA+AGG 0.518559 3:-85830096 MsG0380016624.01.T01:CDS
ATTTGAGTCAGAATCAAAGT+TGG 0.518777 3:-85830104 MsG0380016624.01.T01:CDS
AAGGAAAATAGCACAGAAGA+TGG 0.540540 3:-85829575 MsG0380016624.01.T01:CDS
GAAAGAGTCTTTGCAATCAG+AGG 0.541327 3:-85829906 MsG0380016624.01.T01:intron
AATCATGCAATAGTTCTCAA+TGG 0.553811 3:-85829310 MsG0380016624.01.T01:CDS
AGAAGATAGAAAACAAGAGA+AGG 0.569555 3:-85830162 MsG0380016624.01.T01:CDS
AAACATCGCATGATGGAGGA+AGG 0.574577 3:-85829604 MsG0380016624.01.T01:CDS
AACCATATAGCAACTTGTCT+AGG 0.580258 3:+85830037 None:intergenic
AAGAAGAGAATATTCATGAA+GGG 0.590509 3:-85830252 MsG0380016624.01.T01:CDS
TTCAGAACAGAAGAGCAAGA+TGG 0.602218 3:-85830015 MsG0380016624.01.T01:CDS
TGATGGAGGAAGGGAAGCTA+AGG 0.606332 3:-85829594 MsG0380016624.01.T01:CDS
TCAGAAGCTAAGTTATATGG+TGG 0.615960 3:-85829627 MsG0380016624.01.T01:CDS
ATGGTGGAAACATCGCATGA+TGG 0.617224 3:-85829611 MsG0380016624.01.T01:CDS
AACATCGCATGATGGAGGAA+GGG 0.628539 3:-85829603 MsG0380016624.01.T01:CDS
GTGGAAACATCGCATGATGG+AGG 0.645127 3:-85829608 MsG0380016624.01.T01:CDS
GGCCATATATAATATGATGG+AGG 0.645919 3:-85830275 None:intergenic
GAGCAAGATGGAAATCAAAG+AGG 0.650346 3:-85830003 MsG0380016624.01.T01:CDS
GATTGATCTAACATACCACA+TGG 0.663576 3:+85829329 None:intergenic
GAAAATAGCACAGAAGATGG+TGG 0.663671 3:-85829572 MsG0380016624.01.T01:CDS
AAAATGAAAGAAGCACAACA+TGG 0.668449 3:-85829451 MsG0380016624.01.T01:CDS
ACAGAGAAAATTGAGGACAG+AGG 0.671559 3:-85829428 MsG0380016624.01.T01:CDS
AACATGGACAGAGAAAATTG+AGG 0.710156 3:-85829435 MsG0380016624.01.T01:CDS
TGGTACAATGTTGATCCATG+TGG 0.723453 3:-85829344 MsG0380016624.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGGAAAAAAAAATTCACA+GGG + Chr3:85829902-85829921 None:intergenic 20.0%
! AAGAAGAGAATATTCATGAA+GGG - Chr3:85829302-85829321 MsG0380016624.01.T01:CDS 25.0%
! GTTAGGAAAAAAAAATTCAC+AGG + Chr3:85829903-85829922 None:intergenic 25.0%
!! AAAGCAAAGATTTTCAAATG+AGG - Chr3:85830044-85830063 MsG0380016624.01.T01:CDS 25.0%
!! AAGCAAAGATTTTCAAATGA+GGG - Chr3:85830045-85830064 MsG0380016624.01.T01:CDS 25.0%
!! TTTTTCTTCTTTGAATTCTG+AGG + Chr3:85829370-85829389 None:intergenic 25.0%
!!! GTGAATTTTTTTTTCCTAAC+AGG - Chr3:85829903-85829922 MsG0380016624.01.T01:intron 25.0%
AAGTTAATGTCGAGTCTATA+AGG + Chr3:85829877-85829896 None:intergenic 30.0%
AATATTCATGAAGGGTTCAA+AGG - Chr3:85829310-85829329 MsG0380016624.01.T01:CDS 30.0%
AATCATGCAATAGTTCTCAA+TGG - Chr3:85830244-85830263 MsG0380016624.01.T01:CDS 30.0%
AGAAGATAGAAAACAAGAGA+AGG - Chr3:85829392-85829411 MsG0380016624.01.T01:CDS 30.0%
ATTTGAGTCAGAATCAAAGT+TGG - Chr3:85829450-85829469 MsG0380016624.01.T01:CDS 30.0%
GAAGAAGAGAATATTCATGA+AGG - Chr3:85829301-85829320 MsG0380016624.01.T01:CDS 30.0%
TGACAACAACACTTGAAAAA+TGG - Chr3:85830190-85830209 MsG0380016624.01.T01:CDS 30.0%
TGATGAACAGATAAGATCAT+TGG - Chr3:85829420-85829439 MsG0380016624.01.T01:CDS 30.0%
! AAAATGAAAGAAGCACAACA+TGG - Chr3:85830103-85830122 MsG0380016624.01.T01:CDS 30.0%
! AAGATTTTCAAATGAGGGTT+TGG - Chr3:85830050-85830069 MsG0380016624.01.T01:CDS 30.0%
!!! TTTTCAAGTGTTGTTGTCAA+GGG + Chr3:85830189-85830208 None:intergenic 30.0%
!!! TTTTTCAAGTGTTGTTGTCA+AGG + Chr3:85830190-85830209 None:intergenic 30.0%
AACATGGACAGAGAAAATTG+AGG - Chr3:85830119-85830138 MsG0380016624.01.T01:CDS 35.0%
AACCATATAGCAACTTGTCT+AGG + Chr3:85829520-85829539 None:intergenic 35.0%
AAGACTCTTTCTCTTCCTTA+AGG + Chr3:85829639-85829658 None:intergenic 35.0%
AAGGAAAATAGCACAGAAGA+TGG - Chr3:85829979-85829998 MsG0380016624.01.T01:CDS 35.0%
ACATGCTCAAGCTTAGTATT+AGG - Chr3:85829822-85829841 MsG0380016624.01.T01:intron 35.0%
ATGCAATAGTTCTCAATGGT+TGG - Chr3:85830248-85830267 MsG0380016624.01.T01:CDS 35.0%
GAAATGGATACAACAATTGC+AGG - Chr3:85830010-85830029 MsG0380016624.01.T01:CDS 35.0%
GATTGATCTAACATACCACA+TGG + Chr3:85830228-85830247 None:intergenic 35.0%
GGTTCAGAAGCTAAGTTATA+TGG - Chr3:85829924-85829943 MsG0380016624.01.T01:CDS 35.0%
TCAGAAGCTAAGTTATATGG+TGG - Chr3:85829927-85829946 MsG0380016624.01.T01:CDS 35.0%
TCAGAGGTAAGTTAGTAGTT+TGG - Chr3:85829664-85829683 MsG0380016624.01.T01:intron 35.0%
TTCTAAGTTTCAGTGCCTTA+AGG - Chr3:85829621-85829640 MsG0380016624.01.T01:CDS 35.0%
! ATTTTCAAATGAGGGTTTGG+AGG - Chr3:85830053-85830072 MsG0380016624.01.T01:CDS 35.0%
! GTTCTCAATGGTTGGATTTT+TGG - Chr3:85830256-85830275 MsG0380016624.01.T01:CDS 35.0%
ACAGAGAAAATTGAGGACAG+AGG - Chr3:85830126-85830145 MsG0380016624.01.T01:CDS 40.0%
ACTTAGCTTCTGAACCTGTT+AGG + Chr3:85829920-85829939 None:intergenic 40.0%
AGCCTAGACAAGTTGCTATA+TGG - Chr3:85829515-85829534 MsG0380016624.01.T01:CDS 40.0%
ATGCAACTTGCAAGAGATCT+TGG - Chr3:85829487-85829506 MsG0380016624.01.T01:CDS 40.0%
GAAAATAGCACAGAAGATGG+TGG - Chr3:85829982-85830001 MsG0380016624.01.T01:CDS 40.0%
GAAAGAGTCTTTGCAATCAG+AGG - Chr3:85829648-85829667 MsG0380016624.01.T01:intron 40.0%
GACTCAGCTCGTTAAATACT+CGG - Chr3:85829774-85829793 MsG0380016624.01.T01:intron 40.0%
GAGCAAGATGGAAATCAAAG+AGG - Chr3:85829551-85829570 MsG0380016624.01.T01:CDS 40.0%
TGGTACAATGTTGATCCATG+TGG - Chr3:85830210-85830229 MsG0380016624.01.T01:CDS 40.0%
TTCAGAACAGAAGAGCAAGA+TGG - Chr3:85829539-85829558 MsG0380016624.01.T01:CDS 40.0%
CAGAATCAAAGTTGGAGCCA+AGG - Chr3:85829458-85829477 MsG0380016624.01.T01:CDS 45.0%
GCAAGTTGCATCTTCTTCCT+TGG + Chr3:85829478-85829497 None:intergenic 45.0%
! AAACATCGCATGATGGAGGA+AGG - Chr3:85829950-85829969 MsG0380016624.01.T01:CDS 45.0%
! AACATCGCATGATGGAGGAA+GGG - Chr3:85829951-85829970 MsG0380016624.01.T01:CDS 45.0%
! ATGGTGGAAACATCGCATGA+TGG - Chr3:85829943-85829962 MsG0380016624.01.T01:CDS 45.0%
! GAAGATGGTGGTTCAGGAAA+TGG - Chr3:85829994-85830013 MsG0380016624.01.T01:CDS 45.0%
GTGGAAACATCGCATGATGG+AGG - Chr3:85829946-85829965 MsG0380016624.01.T01:CDS 50.0%
TGATGGAGGAAGGGAAGCTA+AGG - Chr3:85829960-85829979 MsG0380016624.01.T01:CDS 50.0%
! AGCACAGAAGATGGTGGTTC+AGG - Chr3:85829988-85830007 MsG0380016624.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 85829292 85830284 85829292 ID=MsG0380016624.01;Name=MsG0380016624.01
Chr3 mRNA 85829292 85830284 85829292 ID=MsG0380016624.01.T01;Parent=MsG0380016624.01;Name=MsG0380016624.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|245
Chr3 exon 85829907 85830284 85829907 ID=MsG0380016624.01.T01:exon:21952;Parent=MsG0380016624.01.T01
Chr3 exon 85829292 85829651 85829292 ID=MsG0380016624.01.T01:exon:21951;Parent=MsG0380016624.01.T01
Chr3 CDS 85829907 85830284 85829907 ID=MsG0380016624.01.T01:cds;Parent=MsG0380016624.01.T01
Chr3 CDS 85829292 85829651 85829292 ID=MsG0380016624.01.T01:cds;Parent=MsG0380016624.01.T01
Gene Sequence

>MsG0380016624.01.T01

ATGATGGAGGAAGAAGAGAATATTCATGAAGGGTTCAAAGGAAAAAATGTTGAAAGCATGAGTTGCTTAGAGACACCTCAGAATTCAAAGAAGAAAAACAAGAAGATAGAAAACAAGAGAAGGTTTAGTGATGAACAGATAAGATCATTGGAATGCATATTTGAGTCAGAATCAAAGTTGGAGCCAAGGAAGAAGATGCAACTTGCAAGAGATCTTGGTTTGCAGCCTAGACAAGTTGCTATATGGTTTCAGAACAGAAGAGCAAGATGGAAATCAAAGAGGATAGAACAAGAATACAGAAAACTCAAAGATGAATATGATAATTTAGCTTCTAAGTTTCAGTGCCTTAAGGAAGAGAAAGAGTCTTTGCAATCAGAGGTTCAGAAGCTAAGTTATATGGTGGAAACATCGCATGATGGAGGAAGGGAAGCTAAGGAAAATAGCACAGAAGATGGTGGTTCAGGAAATGGATACAACAATTGCAGGTTTGAAGAAACAAAGCAAAGATTTTCAAATGAGGGTTTGGAGGATAAAATCGTCATTTATTCAGATGATCAAAATGAAAGAAGCACAACATGGACAGAGAAAATTGAGGACAGAGGAAATCAACTTTTAAGAAATGATGATCATACAGAAATTCCCTTGACAACAACACTTGAAAAATGGTACAATGTTGATCCATGTGGTATGTTAGATCAATCATGCAATAGTTCTCAATGGTTGGATTTTTGGACTTGA

Protein sequence

>MsG0380016624.01.T01

MMEEEENIHEGFKGKNVESMSCLETPQNSKKKNKKIENKRRFSDEQIRSLECIFESESKLEPRKKMQLARDLGLQPRQVAIWFQNRRARWKSKRIEQEYRKLKDEYDNLASKFQCLKEEKESLQSEVQKLSYMVETSHDGGREAKENSTEDGGSGNGYNNCRFEETKQRFSNEGLEDKIVIYSDDQNERSTTWTEKIEDRGNQLLRNDDHTEIPLTTTLEKWYNVDPCGMLDQSCNSSQWLDFWT*