AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017977.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017977.01.T01 MTR_3g116120 96.970 429 13 0 1 429 1 429 0.0 856
MsG0380017977.01.T01 MTR_5g085170 79.021 429 40 3 1 429 1 379 0.0 651
MsG0380017977.01.T01 MTR_6g053260 61.813 364 52 7 1 363 1 278 3.56e-124 376
MsG0380017977.01.T01 MTR_6g053260 80.000 50 10 0 380 429 234 283 6.63e-16 80.5
MsG0380017977.01.T01 MTR_3g099000 32.424 330 162 7 47 321 6 329 4.07e-46 167
MsG0380017977.01.T01 MTR_4g069830 29.643 280 148 6 51 281 133 412 1.28e-28 119
MsG0380017977.01.T01 MTR_4g069830 29.643 280 148 6 51 281 133 412 1.40e-28 119
MsG0380017977.01.T01 MTR_3g088480 27.296 392 216 14 56 384 170 555 1.81e-28 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017977.01.T01 AT2G33610 52.699 389 162 8 48 426 39 415 7.57e-127 375
MsG0380017977.01.T01 AT2G47620 31.419 296 153 6 51 300 7 298 5.00e-34 132
MsG0380017977.01.T01 AT2G47620 31.419 296 153 6 51 300 7 298 1.37e-33 132
MsG0380017977.01.T01 AT1G21700 26.289 388 211 14 35 361 158 531 4.24e-30 124
MsG0380017977.01.T01 AT4G34430 30.686 277 146 7 51 281 139 415 3.81e-25 108
MsG0380017977.01.T01 AT4G34430 30.686 277 146 7 51 281 139 415 3.81e-25 108
MsG0380017977.01.T01 AT4G34430 30.686 277 146 7 51 281 139 415 3.81e-25 108
MsG0380017977.01.T01 AT4G34430 30.686 277 146 7 51 281 139 415 3.99e-25 108

Find 113 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGCGTCGCTAGAGGTTT+TGG 0.216425 3:+102801536 None:intergenic
CTTGAAGCTATTACGAATTT+CGG 0.268562 3:-102800615 MsG0380017977.01.T01:CDS
GAGGCACGACGGTTCTGTTT+GGG 0.304033 3:+102801664 None:intergenic
GGCTGCTTTCCTTTCTGCTT+TGG 0.304195 3:-102800023 MsG0380017977.01.T01:CDS
GGTTCTGGTAATGGGACTTC+AGG 0.304961 3:+102801558 None:intergenic
TTCTGAGGTTACAGAAGTTC+AGG 0.308719 3:-102799585 MsG0380017977.01.T01:CDS
GCCACCGGAGGCGGCGGTGG+TGG 0.310149 3:+102801585 None:intergenic
TTTGTAAACCCTAGGGTTCT+TGG 0.330782 3:+102801431 None:intergenic
AGGCGGCGGTGGTGGGTTCG+AGG 0.332048 3:+102801593 None:intergenic
GCTTTCCTTTCTGCTTTGGC+AGG 0.337459 3:-102800019 MsG0380017977.01.T01:CDS
TTTATCCGACGCAACAGTTT+CGG 0.342652 3:+102800413 None:intergenic
ATTCGTGGAAATTATCGAAT+TGG 0.350506 3:-102800720 MsG0380017977.01.T01:CDS
CCTAGGGTTCTTGGAAGATT+CGG 0.357797 3:+102801440 None:intergenic
GGAGGCACGACGGTTCTGTT+TGG 0.358696 3:+102801663 None:intergenic
CGCTCAGTAAACCTCTCAAA+TGG 0.370402 3:-102801248 MsG0380017977.01.T01:CDS
CTAGGGTTCTTGGAAGATTC+GGG 0.380857 3:+102801441 None:intergenic
ATTCGATGCTGCGTCGGGTT+TGG 0.382011 3:+102801212 None:intergenic
GACGGTTCTGTTTGGGGTTG+AGG 0.382202 3:+102801671 None:intergenic
AGTCGGAGGTTGAGGTGTTC+CGG 0.392524 3:+102801629 None:intergenic
TTCGCCACCGGAGGCGGCGG+TGG 0.405270 3:+102801582 None:intergenic
TTACGAATTTCGGTGATGAT+TGG 0.419721 3:-102800605 MsG0380017977.01.T01:CDS
CCTTTCTGCTTTGGCAGGGA+CGG 0.421374 3:-102800014 MsG0380017977.01.T01:CDS
CCACCGGAGGCGGCGGTGGT+GGG 0.442186 3:+102801586 None:intergenic
TCAGGTTTCGCCACCGGAGG+CGG 0.443862 3:+102801576 None:intergenic
AAAAGAACCTGAGCCATGAT+AGG 0.445288 3:+102800345 None:intergenic
ACGGATTTCGCATTCGTGAA+TGG 0.445293 3:+102801470 None:intergenic
GGTGGTGGGTTCGAGGGAGG+CGG 0.448251 3:+102801600 None:intergenic
GCGGAATTCGATGCTGCGTC+GGG 0.449596 3:+102801207 None:intergenic
TGGAAGATTCGGGAATGTTA+CGG 0.464080 3:+102801451 None:intergenic
TTGCACGCAATAAAGATCCT+TGG 0.465240 3:+102799651 None:intergenic
AGGGTTGCTCATCAAGTTGT+TGG 0.467288 3:-102800579 MsG0380017977.01.T01:CDS
GAACCCACCACCGCCGCCTC+CGG 0.468860 3:-102801589 MsG0380017977.01.T01:CDS
GTGCCTCCGTCTTCCGCCAC+CGG 0.470261 3:-102801648 MsG0380017977.01.T01:CDS
ATCTGAAGCATTACCACCAT+TGG 0.471038 3:+102799679 None:intergenic
GATTCACATTCAGCATCAAC+GGG 0.474269 3:+102800435 None:intergenic
AATCAACTAGAATTAATTAC+CGG 0.474504 3:-102799937 MsG0380017977.01.T01:CDS
TCTAGTTGATTTGTAAACAT+TGG 0.479041 3:+102799949 None:intergenic
CGAACCAACGTCACCGACTA+GGG 0.480955 3:+102801332 None:intergenic
GGCGGTGGTGGGTTCGAGGG+AGG 0.482062 3:+102801597 None:intergenic
ATTCTCTTAGCTTCTTTGAC+AGG 0.485476 3:+102801165 None:intergenic
CTGTAGTATTTGTAAACCCT+AGG 0.490342 3:+102801423 None:intergenic
TCGAACCAACGTCACCGACT+AGG 0.492371 3:+102801331 None:intergenic
TCTGCTCCTCGCATTGATGA+TGG 0.492996 3:-102800477 MsG0380017977.01.T01:CDS
CCGAATCTTCCAAGAACCCT+AGG 0.500165 3:-102801440 MsG0380017977.01.T01:CDS
GCCACTCAAGATGGTTCTCA+TGG 0.500382 3:-102801497 MsG0380017977.01.T01:CDS
GGTGTTCCGGTGGCGGAAGA+CGG 0.500983 3:+102801642 None:intergenic
AGGTTGAGGTGTTCCGGTGG+CGG 0.501069 3:+102801635 None:intergenic
GGGACTTCAGGTTTCGCCAC+CGG 0.504355 3:+102801570 None:intergenic
TGCGGAATTCGATGCTGCGT+CGG 0.506487 3:+102801206 None:intergenic
GGTTTCGCCACCGGAGGCGG+CGG 0.509368 3:+102801579 None:intergenic
TCTTTGACAGGCGCCGGAGA+AGG 0.514796 3:+102801177 None:intergenic
CTTTCCTTTCTGCTTTGGCA+GGG 0.515547 3:-102800018 MsG0380017977.01.T01:CDS
GATGCTGCTGTTGCATCCAA+TGG 0.519252 3:-102799695 MsG0380017977.01.T01:CDS
GGCTTGCTTTGCCTGTGAGA+AGG 0.520661 3:-102801112 MsG0380017977.01.T01:intron
ACGAGCAACACATTCCTTGT+CGG 0.522938 3:+102800551 None:intergenic
ATCAATGCGAGGAGCAGACT+CGG 0.528293 3:+102800482 None:intergenic
GGATGCTAGCAATCCTATCA+TGG 0.529881 3:-102800358 MsG0380017977.01.T01:CDS
TGCAGAAACTGATCACCAAA+AGG 0.531795 3:+102800516 None:intergenic
AGAACCATCTTGAGTGGCTA+GGG 0.532947 3:+102801502 None:intergenic
TAGCAATCCTATCATGGCTC+AGG 0.533854 3:-102800352 MsG0380017977.01.T01:intron
TCTTTATCTTCCCATTTGAG+AGG 0.534057 3:+102801237 None:intergenic
TGTAGTATTTGTAAACCCTA+GGG 0.534357 3:+102801424 None:intergenic
GGATTCACATTCAGCATCAA+CGG 0.539105 3:+102800434 None:intergenic
GCTCAGTAAACCTCTCAAAT+GGG 0.539806 3:-102801247 MsG0380017977.01.T01:CDS
CCCACCACCGCCGCCTCCGG+TGG 0.540370 3:-102801586 MsG0380017977.01.T01:CDS
TTAGCTTCTTTGACAGGCGC+CGG 0.542076 3:+102801171 None:intergenic
ATTTCGGTGATGATTGGAAG+AGG 0.544103 3:-102800599 MsG0380017977.01.T01:CDS
AACTAAACTGACCTTCTCAC+AGG 0.545557 3:+102801101 None:intergenic
GCTAGGGACTAGTATTAAGT+TGG 0.546014 3:+102801518 None:intergenic
GGTTTACTGAGCGAAGAAGA+AGG 0.548651 3:+102801258 None:intergenic
TTCTGCTTTGGCAGGGACGG+AGG 0.550159 3:-102800011 MsG0380017977.01.T01:CDS
TGCAAATTTACAGCTTGAGA+AGG 0.556628 3:-102799633 MsG0380017977.01.T01:CDS
TTCCGCAGAATCTCCTTCTC+CGG 0.556844 3:-102801190 MsG0380017977.01.T01:CDS
CGAATCTTCCAAGAACCCTA+GGG 0.558609 3:-102801439 MsG0380017977.01.T01:CDS
GGAGTATTAGTCGGAGGTTG+AGG 0.559490 3:+102801621 None:intergenic
CCTTGCTGCAATGTATTCCT+CGG 0.560596 3:+102799908 None:intergenic
ATGTATTCCTCGGAAATGAC+CGG 0.561368 3:+102799918 None:intergenic
TTGCTCATCAAGTTGTTGGT+AGG 0.563248 3:-102800575 MsG0380017977.01.T01:CDS
CCGTCCCTGCCAAAGCAGAA+AGG 0.564962 3:+102800014 None:intergenic
GGCGGCGGTGGTGGGTTCGA+GGG 0.565038 3:+102801594 None:intergenic
AGGCACGACGGTTCTGTTTG+GGG 0.565942 3:+102801665 None:intergenic
TATTAAGTTGGCGTCGCTAG+AGG 0.569207 3:+102801530 None:intergenic
TAATACAGAATTCAAGAGAG+TGG 0.570887 3:-102800691 MsG0380017977.01.T01:CDS
GAGAACCATCTTGAGTGGCT+AGG 0.580360 3:+102801501 None:intergenic
GACGGAGGTTGCACAAGCTG+CGG 0.582049 3:-102799996 MsG0380017977.01.T01:CDS
AATGCTTCAGATTCTATCCA+AGG 0.586687 3:-102799668 MsG0380017977.01.T01:CDS
CAAGAGAGTGGAGATAAGTG+AGG 0.590112 3:-102800679 MsG0380017977.01.T01:CDS
ACTTCAGGTTTCGCCACCGG+AGG 0.593361 3:+102801573 None:intergenic
TAGTCCCTAGCCACTCAAGA+TGG 0.598302 3:-102801506 MsG0380017977.01.T01:CDS
TTAATTACCGGTCATTTCCG+AGG 0.609744 3:-102799925 MsG0380017977.01.T01:CDS
TTTCGGTGATGATTGGAAGA+GGG 0.613337 3:-102800598 MsG0380017977.01.T01:CDS
ACCGGACAAGTGTAAACGAA+TGG 0.614042 3:-102801697 None:intergenic
AATGTGTCTCACACCTCTTG+CGG 0.617103 3:-102800379 MsG0380017977.01.T01:CDS
GGATTGCTAGCATCCGCAAG+AGG 0.621759 3:+102800366 None:intergenic
CCACTCAAGATGGTTCTCAT+GGG 0.624877 3:-102801496 MsG0380017977.01.T01:CDS
CGCCGGAGAAGGAGATTCTG+CGG 0.634496 3:+102801188 None:intergenic
CCGAGGAATACATTGCAGCA+AGG 0.638324 3:-102799908 MsG0380017977.01.T01:intron
TGAATCCGAAACTGTTGCGT+CGG 0.639096 3:-102800418 MsG0380017977.01.T01:CDS
CGGAGGTTGAGGTGTTCCGG+TGG 0.643233 3:+102801632 None:intergenic
CCCATGAGAACCATCTTGAG+TGG 0.645166 3:+102801496 None:intergenic
GTTCCGGTGGCGGAAGACGG+AGG 0.648161 3:+102801645 None:intergenic
GAGGGAGGCGGAGTATTAGT+CGG 0.653700 3:+102801612 None:intergenic
GAAGCTAAGAGAATCTGTAG+TGG 0.654963 3:-102801156 MsG0380017977.01.T01:CDS
AGTTGTTGGTAGGACCGACA+AGG 0.658858 3:-102800565 MsG0380017977.01.T01:CDS
TCACATTCAGCATCAACGGG+AGG 0.659217 3:+102800438 None:intergenic
GATGTTCGCAAAACCCTAGT+CGG 0.661918 3:-102801345 MsG0380017977.01.T01:CDS
ATAATATGACGTTGTGTGCG+AGG 0.667737 3:-102800749 MsG0380017977.01.T01:CDS
TGAGAAGGAAGAATCAGACG+TGG 0.680762 3:-102799618 MsG0380017977.01.T01:CDS
AAAACCCTAGTCGGTGACGT+TGG 0.681388 3:-102801336 MsG0380017977.01.T01:CDS
GGCGGAAGACGGAGGCACGA+CGG 0.688333 3:+102801653 None:intergenic
GCTGCTGTTGCATCCAATGG+TGG 0.695124 3:-102799692 MsG0380017977.01.T01:CDS
TCAGAACCATCATCAATGCG+AGG 0.698974 3:+102800471 None:intergenic
GGAGGCGGAGTATTAGTCGG+AGG 0.718816 3:+102801615 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAAATATTTTCATGATT+TGG + Chr3:102800296-102800315 None:intergenic 10.0%
!!! ATAATTTGAAATGTGAATTT+CGG - Chr3:102800468-102800487 MsG0380017977.01.T01:CDS 15.0%
!! AATCAACTAGAATTAATTAC+CGG - Chr3:102801309-102801328 MsG0380017977.01.T01:CDS 20.0%
!! AATCTGTCATATATCTTAAT+AGG - Chr3:102801418-102801437 MsG0380017977.01.T01:CDS 20.0%
!! ATCTGTCATATATCTTAATA+GGG - Chr3:102801419-102801438 MsG0380017977.01.T01:CDS 20.0%
!! ATTTGTGTGTTATAATGTTT+GGG - Chr3:102800975-102800994 MsG0380017977.01.T01:intron 20.0%
!! CCAATCATATATGTATTTAA+TGG + Chr3:102800385-102800404 None:intergenic 20.0%
!! CCATTAAATACATATATGAT+TGG - Chr3:102800382-102800401 MsG0380017977.01.T01:CDS 20.0%
!! GAAATTCACATTTCAAATTA+TGG + Chr3:102800469-102800488 None:intergenic 20.0%
!! TAAACAATTCAAAGAAAACA+AGG + Chr3:102800941-102800960 None:intergenic 20.0%
!! TTAAAATTAGTTGTATGACA+TGG - Chr3:102800311-102800330 MsG0380017977.01.T01:intron 20.0%
!! TTATTTGTTCAAATTATTGC+AGG - Chr3:102801202-102801221 MsG0380017977.01.T01:CDS 20.0%
!!! AAAATATTTTCATGATTTGG+CGG + Chr3:102800293-102800312 None:intergenic 20.0%
! AAATCATTTGTCATTCAACA+AGG + Chr3:102801156-102801175 None:intergenic 25.0%
! AATCATTTGTCATTCAACAA+GGG + Chr3:102801155-102801174 None:intergenic 25.0%
! AGAACAATTGGAATTTGATT+CGG + Chr3:102801051-102801070 None:intergenic 25.0%
! CATTTGTGTGTTATAATGTT+TGG - Chr3:102800974-102800993 MsG0380017977.01.T01:intron 25.0%
! TTGATTACATGTAGCATTAA+AGG - Chr3:102801016-102801035 MsG0380017977.01.T01:intron 25.0%
! TTTGTGTGTTATAATGTTTG+GGG - Chr3:102800976-102800995 MsG0380017977.01.T01:intron 25.0%
!!! TCTAGTTGATTTGTAAACAT+TGG + Chr3:102801300-102801319 None:intergenic 25.0%
AATCCGAGTTTAAAGATTTC+CGG + Chr3:102801504-102801523 None:intergenic 30.0%
ATTCGTGGAAATTATCGAAT+TGG - Chr3:102800526-102800545 MsG0380017977.01.T01:CDS 30.0%
CTGTCTTAATTCTTAGTAGT+TGG - Chr3:102801078-102801097 MsG0380017977.01.T01:intron 30.0%
CTTGAAGCTATTACGAATTT+CGG - Chr3:102800631-102800650 MsG0380017977.01.T01:CDS 30.0%
GAATCAAATTCCAATTGTTC+TGG - Chr3:102801050-102801069 MsG0380017977.01.T01:intron 30.0%
TAATACAGAATTCAAGAGAG+TGG - Chr3:102800555-102800574 MsG0380017977.01.T01:CDS 30.0%
TAATAGGGTTTATCTCGAAA+TGG - Chr3:102801434-102801453 MsG0380017977.01.T01:CDS 30.0%
TGTAGTATTTGTAAACCCTA+GGG + Chr3:102799825-102799844 None:intergenic 30.0%
! GTACACTTCATTAGTTTGAA+TGG - Chr3:102801476-102801495 MsG0380017977.01.T01:CDS 30.0%
! TCGGATTTTCTCATTAGAAA+TGG - Chr3:102801517-102801536 MsG0380017977.01.T01:CDS 30.0%
! TTCATTAGTTTGAATGGTTC+CGG - Chr3:102801482-102801501 MsG0380017977.01.T01:CDS 30.0%
! TTTTCAGATTCAATCCTAAC+AGG - Chr3:102799863-102799882 MsG0380017977.01.T01:intron 30.0%
!! AAAAAATGAATGGACTGAGA+AGG - Chr3:102800594-102800613 MsG0380017977.01.T01:CDS 30.0%
!! CTTTGATTTTCTTGAAGCTT+GGG - Chr3:102799948-102799967 MsG0380017977.01.T01:CDS 30.0%
!! TCTTTGATTTTCTTGAAGCT+TGG - Chr3:102799947-102799966 MsG0380017977.01.T01:CDS 30.0%
!! TTTGATTTTCTTGAAGCTTG+GGG - Chr3:102799949-102799968 MsG0380017977.01.T01:CDS 30.0%
AAATCCTATAATCTTAGCCC+AGG + Chr3:102801380-102801399 None:intergenic 35.0%
AATGCTTCAGATTCTATCCA+AGG - Chr3:102801578-102801597 MsG0380017977.01.T01:CDS 35.0%
ATAACCTGGGCTAAGATTAT+AGG - Chr3:102801373-102801392 MsG0380017977.01.T01:CDS 35.0%
ATTCTCTTAGCTTCTTTGAC+AGG + Chr3:102800084-102800103 None:intergenic 35.0%
CTGTAGTATTTGTAAACCCT+AGG + Chr3:102799826-102799845 None:intergenic 35.0%
GAATCTGAAAAACTTGACGA+TGG + Chr3:102799854-102799873 None:intergenic 35.0%
GTGAGAATGACATATAACCT+GGG - Chr3:102801360-102801379 MsG0380017977.01.T01:CDS 35.0%
GTGAGGAGACAAAAAATGAA+TGG - Chr3:102800584-102800603 MsG0380017977.01.T01:CDS 35.0%
GTTCCGGAAATCTTTAAACT+CGG - Chr3:102801498-102801517 MsG0380017977.01.T01:CDS 35.0%
TCTTTATCTTCCCATTTGAG+AGG + Chr3:102800012-102800031 None:intergenic 35.0%
TGCAAATTTACAGCTTGAGA+AGG - Chr3:102801613-102801632 MsG0380017977.01.T01:CDS 35.0%
TTACGAATTTCGGTGATGAT+TGG - Chr3:102800641-102800660 MsG0380017977.01.T01:CDS 35.0%
! GTGAATGTGATTTTCCTGTT+AGG + Chr3:102799880-102799899 None:intergenic 35.0%
!!! CGTTTTTTAGAGCTTCCTTT+TGG - Chr3:102800715-102800734 MsG0380017977.01.T01:CDS 35.0%
!!! TGGCGGAATACAAAGTTTTT+TGG + Chr3:102800276-102800295 None:intergenic 35.0%
AAAAGAACCTGAGCCATGAT+AGG + Chr3:102800904-102800923 None:intergenic 40.0%
AACTAAACTGACCTTCTCAC+AGG + Chr3:102800148-102800167 None:intergenic 40.0%
ATAATATGACGTTGTGTGCG+AGG - Chr3:102800497-102800516 MsG0380017977.01.T01:CDS 40.0%
ATGTATTCCTCGGAAATGAC+CGG + Chr3:102801331-102801350 None:intergenic 40.0%
ATTTCGGTGATGATTGGAAG+AGG - Chr3:102800647-102800666 MsG0380017977.01.T01:CDS 40.0%
CGTGGAAAAAGCAATTTCTG+AGG - Chr3:102801646-102801665 MsG0380017977.01.T01:CDS 40.0%
CTGCAAAAATCTCTGTGTCA+TGG - Chr3:102800113-102800132 MsG0380017977.01.T01:intron 40.0%
GAAGCTAAGAGAATCTGTAG+TGG - Chr3:102800090-102800109 MsG0380017977.01.T01:intron 40.0%
GAAGTGTACACGATGAACAT+TGG + Chr3:102801465-102801484 None:intergenic 40.0%
GATTCACATTCAGCATCAAC+GGG + Chr3:102800814-102800833 None:intergenic 40.0%
GCTCAGTAAACCTCTCAAAT+GGG - Chr3:102799999-102800018 MsG0380017977.01.T01:CDS 40.0%
GGATTCACATTCAGCATCAA+CGG + Chr3:102800815-102800834 None:intergenic 40.0%
GGTGAGAATGACATATAACC+TGG - Chr3:102801359-102801378 MsG0380017977.01.T01:CDS 40.0%
TGCAGAAACTGATCACCAAA+AGG + Chr3:102800733-102800752 None:intergenic 40.0%
TGGAAGATTCGGGAATGTTA+CGG + Chr3:102799798-102799817 None:intergenic 40.0%
TGTATGACATGGCAGATTGA+TGG - Chr3:102800322-102800341 MsG0380017977.01.T01:intron 40.0%
TTAATGACTGTGCTCTGACA+CGG - Chr3:102801104-102801123 MsG0380017977.01.T01:intron 40.0%
TTAATTACCGGTCATTTCCG+AGG - Chr3:102801321-102801340 MsG0380017977.01.T01:CDS 40.0%
TTGCACGCAATAAAGATCCT+TGG + Chr3:102801598-102801617 None:intergenic 40.0%
TTTATCCGACGCAACAGTTT+CGG + Chr3:102800836-102800855 None:intergenic 40.0%
TTTCGGTGATGATTGGAAGA+GGG - Chr3:102800648-102800667 MsG0380017977.01.T01:CDS 40.0%
TTTGTAAACCCTAGGGTTCT+TGG + Chr3:102799818-102799837 None:intergenic 40.0%
TTTGTCATTCAACAAGGGAC+TGG + Chr3:102801150-102801169 None:intergenic 40.0%
! ATCATGGCTCAGGTTCTTTT+TGG - Chr3:102800904-102800923 MsG0380017977.01.T01:intron 40.0%
! ATCTGAAGCATTACCACCAT+TGG + Chr3:102801570-102801589 None:intergenic 40.0%
! GCAGATTGATGGTTTCTTAG+CGG - Chr3:102800333-102800352 MsG0380017977.01.T01:intron 40.0%
! GCTAGGGACTAGTATTAAGT+TGG + Chr3:102799731-102799750 None:intergenic 40.0%
! TTGCTCATCAAGTTGTTGGT+AGG - Chr3:102800671-102800690 MsG0380017977.01.T01:CDS 40.0%
!! TTCTGAGGTTACAGAAGTTC+AGG - Chr3:102801661-102801680 MsG0380017977.01.T01:CDS 40.0%
!!! AGGTTTTGGGTTCTGGTAAT+GGG + Chr3:102799699-102799718 None:intergenic 40.0%
AATGTGTCTCACACCTCTTG+CGG - Chr3:102800867-102800886 MsG0380017977.01.T01:intron 45.0%
ACGAGCAACACATTCCTTGT+CGG + Chr3:102800698-102800717 None:intergenic 45.0%
ACGGATTTCGCATTCGTGAA+TGG + Chr3:102799779-102799798 None:intergenic 45.0%
AGAACCATCTTGAGTGGCTA+GGG + Chr3:102799747-102799766 None:intergenic 45.0%
CAAGAGAGTGGAGATAAGTG+AGG - Chr3:102800567-102800586 MsG0380017977.01.T01:CDS 45.0%
CCACTCAAGATGGTTCTCAT+GGG - Chr3:102799750-102799769 MsG0380017977.01.T01:intron 45.0%
CCTAGGGTTCTTGGAAGATT+CGG + Chr3:102799809-102799828 None:intergenic 45.0%
CCTTGCTGCAATGTATTCCT+CGG + Chr3:102801341-102801360 None:intergenic 45.0%
CGAATCTTCCAAGAACCCTA+GGG - Chr3:102799807-102799826 MsG0380017977.01.T01:intron 45.0%
CGCTCAGTAAACCTCTCAAA+TGG - Chr3:102799998-102800017 MsG0380017977.01.T01:CDS 45.0%
CTAGGGTTCTTGGAAGATTC+GGG + Chr3:102799808-102799827 None:intergenic 45.0%
CTTTCCTTTCTGCTTTGGCA+GGG - Chr3:102801228-102801247 MsG0380017977.01.T01:CDS 45.0%
GACAGCGATTCCAGAACAAT+TGG + Chr3:102801063-102801082 None:intergenic 45.0%
GATGTTCGCAAAACCCTAGT+CGG - Chr3:102799901-102799920 MsG0380017977.01.T01:intron 45.0%
GGATGCTAGCAATCCTATCA+TGG - Chr3:102800888-102800907 MsG0380017977.01.T01:intron 45.0%
GGTTTACTGAGCGAAGAAGA+AGG + Chr3:102799991-102800010 None:intergenic 45.0%
TAGCAATCCTATCATGGCTC+AGG - Chr3:102800894-102800913 MsG0380017977.01.T01:intron 45.0%
TCAGAACCATCATCAATGCG+AGG + Chr3:102800778-102800797 None:intergenic 45.0%
TGAATCCGAAACTGTTGCGT+CGG - Chr3:102800828-102800847 MsG0380017977.01.T01:intron 45.0%
TGAGAAGGAAGAATCAGACG+TGG - Chr3:102801628-102801647 MsG0380017977.01.T01:CDS 45.0%
! AGGGTTGCTCATCAAGTTGT+TGG - Chr3:102800667-102800686 MsG0380017977.01.T01:CDS 45.0%
! GACTAGGGTTTTGCGAACAT+CGG + Chr3:102799902-102799921 None:intergenic 45.0%
!! TATTAAGTTGGCGTCGCTAG+AGG + Chr3:102799719-102799738 None:intergenic 45.0%
!! TGTGCGAGGTGTTTTATTCG+TGG - Chr3:102800511-102800530 MsG0380017977.01.T01:CDS 45.0%
!!! GAGGTTTTGGGTTCTGGTAA+TGG + Chr3:102799700-102799719 None:intergenic 45.0%
AAAACCCTAGTCGGTGACGT+TGG - Chr3:102799910-102799929 MsG0380017977.01.T01:CDS 50.0%
ATCAATGCGAGGAGCAGACT+CGG + Chr3:102800767-102800786 None:intergenic 50.0%
CCCATGAGAACCATCTTGAG+TGG + Chr3:102799753-102799772 None:intergenic 50.0%
CCGAATCTTCCAAGAACCCT+AGG - Chr3:102799806-102799825 MsG0380017977.01.T01:intron 50.0%
CCGAGGAATACATTGCAGCA+AGG - Chr3:102801338-102801357 MsG0380017977.01.T01:CDS 50.0%
GAGAACCATCTTGAGTGGCT+AGG + Chr3:102799748-102799767 None:intergenic 50.0%
GATGCTGCTGTTGCATCCAA+TGG - Chr3:102801551-102801570 MsG0380017977.01.T01:CDS 50.0%
GCCACTCAAGATGGTTCTCA+TGG - Chr3:102799749-102799768 MsG0380017977.01.T01:intron 50.0%
GCTTTCCTTTCTGCTTTGGC+AGG - Chr3:102801227-102801246 MsG0380017977.01.T01:CDS 50.0%
TAGTCCCTAGCCACTCAAGA+TGG - Chr3:102799740-102799759 MsG0380017977.01.T01:intron 50.0%
TCACATTCAGCATCAACGGG+AGG + Chr3:102800811-102800830 None:intergenic 50.0%
TTAGCTTCTTTGACAGGCGC+CGG + Chr3:102800078-102800097 None:intergenic 50.0%
TTCCGCAGAATCTCCTTCTC+CGG - Chr3:102800056-102800075 MsG0380017977.01.T01:intron 50.0%
! AGTTGTTGGTAGGACCGACA+AGG - Chr3:102800681-102800700 MsG0380017977.01.T01:CDS 50.0%
! GGAGTATTAGTCGGAGGTTG+AGG + Chr3:102799628-102799647 None:intergenic 50.0%
! GGCTGCTTTCCTTTCTGCTT+TGG - Chr3:102801223-102801242 MsG0380017977.01.T01:CDS 50.0%
! TCTGCTCCTCGCATTGATGA+TGG - Chr3:102800769-102800788 MsG0380017977.01.T01:intron 50.0%
! TTGGCGTCGCTAGAGGTTTT+GGG + Chr3:102799712-102799731 None:intergenic 50.0%
!! GGTTCTGGTAATGGGACTTC+AGG + Chr3:102799691-102799710 None:intergenic 50.0%
!!! TCGCTAGAGGTTTTGGGTTC+TGG + Chr3:102799706-102799725 None:intergenic 50.0%
AGGCACGACGGTTCTGTTTG+GGG + Chr3:102799584-102799603 None:intergenic 55.0%
ATTCGATGCTGCGTCGGGTT+TGG + Chr3:102800037-102800056 None:intergenic 55.0%
CCTTTCTGCTTTGGCAGGGA+CGG - Chr3:102801232-102801251 MsG0380017977.01.T01:CDS 55.0%
GAGGCACGACGGTTCTGTTT+GGG + Chr3:102799585-102799604 None:intergenic 55.0%
GCTGCTGTTGCATCCAATGG+TGG - Chr3:102801554-102801573 MsG0380017977.01.T01:CDS 55.0%
GGATTGCTAGCATCCGCAAG+AGG + Chr3:102800883-102800902 None:intergenic 55.0%
TGCGGAATTCGATGCTGCGT+CGG + Chr3:102800043-102800062 None:intergenic 55.0%
! AGTCGGAGGTTGAGGTGTTC+CGG + Chr3:102799620-102799639 None:intergenic 55.0%
! CGAACCAACGTCACCGACTA+GGG + Chr3:102799917-102799936 None:intergenic 55.0%
! GACGGTTCTGTTTGGGGTTG+AGG + Chr3:102799578-102799597 None:intergenic 55.0%
! GAGGGAGGCGGAGTATTAGT+CGG + Chr3:102799637-102799656 None:intergenic 55.0%
! GGCTTGCTTTGCCTGTGAGA+AGG - Chr3:102800134-102800153 MsG0380017977.01.T01:intron 55.0%
! GTTGGCGTCGCTAGAGGTTT+TGG + Chr3:102799713-102799732 None:intergenic 55.0%
! TCGAACCAACGTCACCGACT+AGG + Chr3:102799918-102799937 None:intergenic 55.0%
CCGTCCCTGCCAAAGCAGAA+AGG + Chr3:102801235-102801254 None:intergenic 60.0%
CGCCGGAGAAGGAGATTCTG+CGG + Chr3:102800061-102800080 None:intergenic 60.0%
GACGGAGGTTGCACAAGCTG+CGG - Chr3:102801250-102801269 MsG0380017977.01.T01:CDS 60.0%
GCGGAATTCGATGCTGCGTC+GGG + Chr3:102800042-102800061 None:intergenic 60.0%
TTCTGCTTTGGCAGGGACGG+AGG - Chr3:102801235-102801254 MsG0380017977.01.T01:CDS 60.0%
! ACTTCAGGTTTCGCCACCGG+AGG + Chr3:102799676-102799695 None:intergenic 60.0%
! AGGTTGAGGTGTTCCGGTGG+CGG + Chr3:102799614-102799633 None:intergenic 60.0%
! GGAGGCACGACGGTTCTGTT+TGG + Chr3:102799586-102799605 None:intergenic 60.0%
! GGAGGCGGAGTATTAGTCGG+AGG + Chr3:102799634-102799653 None:intergenic 60.0%
! GGGACTTCAGGTTTCGCCAC+CGG + Chr3:102799679-102799698 None:intergenic 60.0%
!! TCTTTGACAGGCGCCGGAGA+AGG + Chr3:102800072-102800091 None:intergenic 60.0%
TCAGGTTTCGCCACCGGAGG+CGG + Chr3:102799673-102799692 None:intergenic 65.0%
! CGGAGGTTGAGGTGTTCCGG+TGG + Chr3:102799617-102799636 None:intergenic 65.0%
!! GGTGTTCCGGTGGCGGAAGA+CGG + Chr3:102799607-102799626 None:intergenic 65.0%
GTGCCTCCGTCTTCCGCCAC+CGG - Chr3:102799598-102799617 MsG0380017977.01.T01:CDS 70.0%
! GGCGGAAGACGGAGGCACGA+CGG + Chr3:102799596-102799615 None:intergenic 70.0%
!! GGTGGTGGGTTCGAGGGAGG+CGG + Chr3:102799649-102799668 None:intergenic 70.0%
!! GTTCCGGTGGCGGAAGACGG+AGG + Chr3:102799604-102799623 None:intergenic 70.0%
GAACCCACCACCGCCGCCTC+CGG - Chr3:102799657-102799676 MsG0380017977.01.T01:CDS 75.0%
GGTTTCGCCACCGGAGGCGG+CGG + Chr3:102799670-102799689 None:intergenic 75.0%
!! AGGCGGCGGTGGTGGGTTCG+AGG + Chr3:102799656-102799675 None:intergenic 75.0%
!! GGCGGCGGTGGTGGGTTCGA+GGG + Chr3:102799655-102799674 None:intergenic 75.0%
!! GGCGGTGGTGGGTTCGAGGG+AGG + Chr3:102799652-102799671 None:intergenic 75.0%
! CCACCGGAGGCGGCGGTGGT+GGG + Chr3:102799663-102799682 None:intergenic 80.0%
! TTCGCCACCGGAGGCGGCGG+TGG + Chr3:102799667-102799686 None:intergenic 80.0%
! CCCACCACCGCCGCCTCCGG+TGG - Chr3:102799660-102799679 MsG0380017977.01.T01:CDS 85.0%
! GCCACCGGAGGCGGCGGTGG+TGG + Chr3:102799664-102799683 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr3 gene 102799562 102801706 102799562 ID=MsG0380017977.01;Name=MsG0380017977.01
Chr3 mRNA 102799562 102801706 102799562 ID=MsG0380017977.01.T01;Parent=MsG0380017977.01;Name=MsG0380017977.01.T01;_AED=0.42;_eAED=0.42;_QI=6|1|0.75|1|1|1|4|0|432
Chr3 exon 102799562 102799716 102799562 ID=MsG0380017977.01.T01:exon:18321;Parent=MsG0380017977.01.T01
Chr3 exon 102799909 102800044 102799909 ID=MsG0380017977.01.T01:exon:18320;Parent=MsG0380017977.01.T01
Chr3 exon 102800353 102800772 102800353 ID=MsG0380017977.01.T01:exon:18319;Parent=MsG0380017977.01.T01
Chr3 exon 102801113 102801706 102801113 ID=MsG0380017977.01.T01:exon:18318;Parent=MsG0380017977.01.T01
Chr3 five_prime_UTR 102801701 102801706 102801701 ID=MsG0380017977.01.T01:five_prime_utr;Parent=MsG0380017977.01.T01
Chr3 CDS 102801113 102801700 102801113 ID=MsG0380017977.01.T01:cds;Parent=MsG0380017977.01.T01
Chr3 CDS 102800353 102800772 102800353 ID=MsG0380017977.01.T01:cds;Parent=MsG0380017977.01.T01
Chr3 CDS 102799909 102800044 102799909 ID=MsG0380017977.01.T01:cds;Parent=MsG0380017977.01.T01
Chr3 CDS 102799562 102799716 102799562 ID=MsG0380017977.01.T01:cds;Parent=MsG0380017977.01.T01
Gene Sequence

>MsG0380017977.01.T01

ATGGCGACCTCAACCCCAAACAGAACCGTCGTGCCTCCGTCTTCCGCCACCGGAACACCTCAACCTCCGACTAATACTCCGCCTCCCTCGAACCCACCACCGCCGCCTCCGGTGGCGAAACCTGAAGTCCCATTACCAGAACCCAAAACCTCTAGCGACGCCAACTTAATACTAGTCCCTAGCCACTCAAGATGGTTCTCATGGGATTCCATTCACGAATGCGAAATCCGTAACATTCCCGAATCTTCCAAGAACCCTAGGGTTTACAAATACTACAGAAACTCCATCGTCAAGTTTTTCAGATTCAATCCTAACAGGAAAATCACATTCACCGATGTTCGCAAAACCCTAGTCGGTGACGTTGGTTCGATTCGAAGAGTCTTTGATTTTCTTGAAGCTTGGGGTTTAATCAATTATCATCCTTCTTCTTCGCTCAGTAAACCTCTCAAATGGGAAGATAAAGACACCAAACCCGACGCAGCATCGAATTCCGCAGAATCTCCTTCTCCGGCGCCTGTCAAAGAAGCTAAGAGAATCTGTAGTGGCTGCAAAAATCTCTGTGTCATGGCTTGCTTTGCCTGTGAGAAGAATAATATGACGTTGTGTGCGAGGTGTTTTATTCGTGGAAATTATCGAATTGGTATGAGTAATACAGAATTCAAGAGAGTGGAGATAAGTGAGGAGACAAAAAATGAATGGACTGAGAAGGAAACACTGAATCTTCTTGAAGCTATTACGAATTTCGGTGATGATTGGAAGAGGGTTGCTCATCAAGTTGTTGGTAGGACCGACAAGGAATGTGTTGCTCGTTTTTTAGAGCTTCCTTTTGGTGATCAGTTTCTGCATTATCAACACTCCGAGTCTGCTCCTCGCATTGATGATGGTTCTGATCAGTTGAAGCCTCCCGTTGATGCTGAATGTGAATCCGAAACTGTTGCGTCGGATAAATCTAGTAAGAGAATGTGTCTCACACCTCTTGCGGATGCTAGCAATCCTATCATGGCTCAGGCTGCTTTCCTTTCTGCTTTGGCAGGGACGGAGGTTGCACAAGCTGCGGCTCAAGCTGCTCTGACAAGTTTGTCCAATGTTTACAAATCAACTAGAATTAATTACCGGTCATTTCCGAGGAATACATTGCAGCAAGATGCTGCTGTTGCATCCAATGGTGGTAATGCTTCAGATTCTATCCAAGGATCTTTATTGCGTGCAAATTTACAGCTTGAGAAGGAAGAATCAGACGTGGAAAAAGCAATTTCTGAGGTTACAGAAGTTCAGGTCTGTCATGTGTTAGCGATTTAG
Protein sequence

>MsG0380017977.01.T01

MATSTPNRTVVPPSSATGTPQPPTNTPPPSNPPPPPPVAKPEVPLPEPKTSSDANLILVPSHSRWFSWDSIHECEIRNIPESSKNPRVYKYYRNSIVKFFRFNPNRKITFTDVRKTLVGDVGSIRRVFDFLEAWGLINYHPSSSLSKPLKWEDKDTKPDAASNSAESPSPAPVKEAKRICSGCKNLCVMACFACEKNNMTLCARCFIRGNYRIGMSNTEFKRVEISEETKNEWTEKETLNLLEAITNFGDDWKRVAHQVVGRTDKECVARFLELPFGDQFLHYQHSESAPRIDDGSDQLKPPVDAECESETVASDKSSKRMCLTPLADASNPIMAQAAFLSALAGTEVAQAAAQAALTSLSNVYKSTRINYRSFPRNTLQQDAAVASNGGNASDSIQGSLLRANLQLEKEESDVEKAISEVTEVQVCHVLAI*