AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036534.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036534.01.T01 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0780036534.01.T01 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.15e-95 275
MsG0780036534.01.T01 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0780036534.01.T01 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0780036534.01.T01 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0780036534.01.T01 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.44e-92 264
MsG0780036534.01.T01 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.38e-91 262
MsG0780036534.01.T01 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0780036534.01.T01 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0780036534.01.T01 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.82e-82 238
MsG0780036534.01.T01 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.64e-75 219
MsG0780036534.01.T01 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.05e-65 195
MsG0780036534.01.T01 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.74e-56 171
MsG0780036534.01.T01 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.96e-52 160
MsG0780036534.01.T01 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.69e-45 144
MsG0780036534.01.T01 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.67e-43 139
MsG0780036534.01.T01 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.74e-42 137
MsG0780036534.01.T01 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.50e-40 132
MsG0780036534.01.T01 MTR_8g027840 55.102 98 43 1 37 133 28 125 5.01e-30 105
MsG0780036534.01.T01 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.48e-25 92.4
MsG0780036534.01.T01 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.75e-20 80.9
MsG0780036534.01.T01 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.26e-18 73.6
MsG0780036534.01.T01 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.81e-15 66.2
MsG0780036534.01.T01 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.48e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036534.01.T01 AT5G65360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0780036534.01.T01 AT5G10390 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0780036534.01.T01 AT5G10400 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0780036534.01.T01 AT3G27360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0780036534.01.T01 AT1G09200 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0780036534.01.T01 AT5G10980 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0780036534.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0780036534.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0780036534.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0780036534.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0780036534.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0780036534.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0780036534.01.T01 AT4G40030 97.059 136 4 0 1 136 29 164 7.04e-93 266
MsG0780036534.01.T01 AT5G65350 94.853 136 7 0 1 136 1 136 5.30e-91 260
MsG0780036534.01.T01 AT1G75600 93.382 136 9 0 1 136 1 136 1.25e-89 256
MsG0780036534.01.T01 AT1G13370 93.382 136 9 0 1 136 1 136 5.55e-89 254
MsG0780036534.01.T01 AT1G19890 89.781 137 13 1 1 136 1 137 1.07e-83 241
MsG0780036534.01.T01 AT5G12910 71.111 135 34 2 1 135 1 130 9.89e-67 198
MsG0780036534.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0780036534.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0780036534.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0780036534.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 27 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTCACGAACCAATCTT+TGG 0.247437 7:+9430566 None:intergenic
TTCTTCACTCCTCCGGTTGC+AGG 0.260302 7:+9430679 None:intergenic
TCACGAAGCGCAACTGTTCC+TGG 0.296792 7:+9430640 None:intergenic
GAAACTTCCATTCCAAAGAT+TGG 0.376853 7:-9430578 MsG0780036534.01.T01:CDS
GGACTTTCTTGCTGTTTGTT+TGG 0.400331 7:+9430759 None:intergenic
GCCGCTGAAGCGTATCTTGT+TGG 0.409289 7:-9430484 MsG0780036534.01.T01:CDS
CCGCTGAAGCGTATCTTGTT+GGG 0.413778 7:-9430483 MsG0780036534.01.T01:CDS
CTTGCAAGTTGAATATCCTT+AGG 0.413826 7:+9430406 None:intergenic
GAGCAGAAACAGCACTGCTC+TGG 0.422418 7:+9430515 None:intergenic
AGAGCACTGAGCTTCTCATT+AGG 0.445355 7:-9430600 MsG0780036534.01.T01:CDS
AGAAAATCAGCTCCTGCAAC+CGG 0.461012 7:-9430691 MsG0780036534.01.T01:CDS
TGGTGGCGAGTTGTTTCCGT+GGG 0.469293 7:+9430722 None:intergenic
GTGTGGCTTCTTCACTCCTC+CGG 0.469362 7:+9430672 None:intergenic
AAGAGTTACTATTATGCCTA+AGG 0.508627 7:-9430422 MsG0780036534.01.T01:CDS
TCACGAACCAATCTTTGGAA+TGG 0.535409 7:+9430571 None:intergenic
TTGGTGGCGAGTTGTTTCCG+TGG 0.577381 7:+9430721 None:intergenic
CAGTGCTCTTCTGATACTTG+CGG 0.590815 7:+9430614 None:intergenic
CAAACAGCAAGAAAGTCCAC+CGG 0.628037 7:-9430754 MsG0780036534.01.T01:CDS
CCCAACAAGATACGCTTCAG+CGG 0.629084 7:+9430483 None:intergenic
CCACCGGAGGAAAAGCCCCA+CGG 0.632336 7:-9430738 MsG0780036534.01.T01:CDS
AAGCCACACAGATTCCGTCC+AGG 0.632383 7:-9430658 MsG0780036534.01.T01:CDS
AAATCAGCTCCTGCAACCGG+AGG 0.655480 7:-9430688 MsG0780036534.01.T01:CDS
CAACTTGCAAGAAGAATCAG+AGG 0.660238 7:-9430394 MsG0780036534.01.T01:CDS
GAAGCGCAACTGTTCCTGGA+CGG 0.668213 7:+9430644 None:intergenic
ACAGCAAGAAAGTCCACCGG+AGG 0.672804 7:-9430751 MsG0780036534.01.T01:CDS
GTTCCTGGACGGAATCTGTG+TGG 0.689851 7:+9430655 None:intergenic
GGTGGCGAGTTGTTTCCGTG+GGG 0.739791 7:+9430723 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAGTTACTATTATGCCTA+AGG - Chr7:9430728-9430747 MsG0780036534.01.T01:CDS 30.0%
GAAACTTCCATTCCAAAGAT+TGG - Chr7:9430572-9430591 MsG0780036534.01.T01:CDS 35.0%
! CTTGCAAGTTGAATATCCTT+AGG + Chr7:9430747-9430766 None:intergenic 35.0%
CAACTTGCAAGAAGAATCAG+AGG - Chr7:9430756-9430775 MsG0780036534.01.T01:CDS 40.0%
CGATTTCACGAACCAATCTT+TGG + Chr7:9430587-9430606 None:intergenic 40.0%
! TCACGAACCAATCTTTGGAA+TGG + Chr7:9430582-9430601 None:intergenic 40.0%
!! GGACTTTCTTGCTGTTTGTT+TGG + Chr7:9430394-9430413 None:intergenic 40.0%
AGAAAATCAGCTCCTGCAAC+CGG - Chr7:9430459-9430478 MsG0780036534.01.T01:CDS 45.0%
CAAACAGCAAGAAAGTCCAC+CGG - Chr7:9430396-9430415 MsG0780036534.01.T01:CDS 45.0%
CAGTGCTCTTCTGATACTTG+CGG + Chr7:9430539-9430558 None:intergenic 45.0%
!! AGAGCACTGAGCTTCTCATT+AGG - Chr7:9430550-9430569 MsG0780036534.01.T01:CDS 45.0%
!! AGCTGATTTTCTTGCGGCTT+TGG + Chr7:9430451-9430470 None:intergenic 45.0%
!! TGATTTTCTTGCGGCTTTGG+TGG + Chr7:9430448-9430467 None:intergenic 45.0%
!! TGCAGGAGCTGATTTTCTTG+CGG + Chr7:9430457-9430476 None:intergenic 45.0%
CCCAACAAGATACGCTTCAG+CGG + Chr7:9430670-9430689 None:intergenic 50.0%
CCGCTGAAGCGTATCTTGTT+GGG - Chr7:9430667-9430686 MsG0780036534.01.T01:CDS 50.0%
AAATCAGCTCCTGCAACCGG+AGG - Chr7:9430462-9430481 MsG0780036534.01.T01:CDS 55.0%
AAGCCACACAGATTCCGTCC+AGG - Chr7:9430492-9430511 MsG0780036534.01.T01:CDS 55.0%
ACAGCAAGAAAGTCCACCGG+AGG - Chr7:9430399-9430418 MsG0780036534.01.T01:CDS 55.0%
GAAGCGCAACTGTTCCTGGA+CGG + Chr7:9430509-9430528 None:intergenic 55.0%
GAGCAGAAACAGCACTGCTC+TGG + Chr7:9430638-9430657 None:intergenic 55.0%
GCCGCTGAAGCGTATCTTGT+TGG - Chr7:9430666-9430685 MsG0780036534.01.T01:CDS 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + Chr7:9430481-9430500 None:intergenic 55.0%
GTTCCTGGACGGAATCTGTG+TGG + Chr7:9430498-9430517 None:intergenic 55.0%
TCACGAAGCGCAACTGTTCC+TGG + Chr7:9430513-9430532 None:intergenic 55.0%
TTCTTCACTCCTCCGGTTGC+AGG + Chr7:9430474-9430493 None:intergenic 55.0%
! TGGTGGCGAGTTGTTTCCGT+GGG + Chr7:9430431-9430450 None:intergenic 55.0%
! TTTCCGTGGGGCTTTTCCTC+CGG + Chr7:9430418-9430437 None:intergenic 55.0%
!! TTGGTGGCGAGTTGTTTCCG+TGG + Chr7:9430432-9430451 None:intergenic 55.0%
! GGTGGCGAGTTGTTTCCGTG+GGG + Chr7:9430430-9430449 None:intergenic 60.0%
CCACCGGAGGAAAAGCCCCA+CGG - Chr7:9430412-9430431 MsG0780036534.01.T01:CDS 65.0%
! CCGTGGGGCTTTTCCTCCGG+TGG + Chr7:9430415-9430434 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 9430381 9430791 9430381 ID=MsG0780036534.01;Name=MsG0780036534.01
Chr7 mRNA 9430381 9430791 9430381 ID=MsG0780036534.01.T01;Parent=MsG0780036534.01;Name=MsG0780036534.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|136
Chr7 exon 9430381 9430791 9430381 ID=MsG0780036534.01.T01:exon:10317;Parent=MsG0780036534.01.T01
Chr7 CDS 9430381 9430791 9430381 ID=MsG0780036534.01.T01:cds;Parent=MsG0780036534.01.T01
Gene Sequence

>MsG0780036534.01.T01

ATGGCGCGTACCAAACAAACAGCAAGAAAGTCCACCGGAGGAAAAGCCCCACGGAAACAACTCGCCACCAAAGCCGCAAGAAAATCAGCTCCTGCAACCGGAGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACAGTTGCGCTTCGTGAGATCCGCAAGTATCAGAAGAGCACTGAGCTTCTCATTAGGAAACTTCCATTCCAAAGATTGGTTCGTGAAATCGCTCAAGATTTCAAAACTGATCTCAGATTCCAGAGCAGTGCTGTTTCTGCTCTTCAAGAAGCCGCTGAAGCGTATCTTGTTGGGTTGTTTGAAGATACGAATCTTTGCGCTATTCATGCTAAAAGAGTTACTATTATGCCTAAGGATATTCAACTTGCAAGAAGAATCAGAGGCGAAAGAGCTTAG

Protein sequence

>MsG0780036534.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*