AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022307.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022307.01.T01 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480022307.01.T01 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.15e-95 275
MsG0480022307.01.T01 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0480022307.01.T01 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0480022307.01.T01 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0480022307.01.T01 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.44e-92 264
MsG0480022307.01.T01 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.38e-91 262
MsG0480022307.01.T01 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0480022307.01.T01 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0480022307.01.T01 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.82e-82 238
MsG0480022307.01.T01 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.64e-75 219
MsG0480022307.01.T01 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.05e-65 195
MsG0480022307.01.T01 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.74e-56 171
MsG0480022307.01.T01 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.96e-52 160
MsG0480022307.01.T01 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.69e-45 144
MsG0480022307.01.T01 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.67e-43 139
MsG0480022307.01.T01 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.74e-42 137
MsG0480022307.01.T01 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.50e-40 132
MsG0480022307.01.T01 MTR_8g027840 55.102 98 43 1 37 133 28 125 5.01e-30 105
MsG0480022307.01.T01 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.48e-25 92.4
MsG0480022307.01.T01 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.75e-20 80.9
MsG0480022307.01.T01 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.26e-18 73.6
MsG0480022307.01.T01 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.81e-15 66.2
MsG0480022307.01.T01 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.48e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022307.01.T01 AT5G65360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480022307.01.T01 AT5G10390 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480022307.01.T01 AT5G10400 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480022307.01.T01 AT3G27360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480022307.01.T01 AT1G09200 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480022307.01.T01 AT5G10980 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480022307.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480022307.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480022307.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480022307.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480022307.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480022307.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480022307.01.T01 AT4G40030 97.059 136 4 0 1 136 29 164 7.04e-93 266
MsG0480022307.01.T01 AT5G65350 94.853 136 7 0 1 136 1 136 5.30e-91 260
MsG0480022307.01.T01 AT1G75600 93.382 136 9 0 1 136 1 136 1.25e-89 256
MsG0480022307.01.T01 AT1G13370 93.382 136 9 0 1 136 1 136 5.55e-89 254
MsG0480022307.01.T01 AT1G19890 89.781 137 13 1 1 136 1 137 1.07e-83 241
MsG0480022307.01.T01 AT5G12910 71.111 135 34 2 1 135 1 130 9.89e-67 198
MsG0480022307.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0480022307.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0480022307.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0480022307.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 52 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAAACCTCACCGTTTC+AGG 0.224781 4:+70966788 MsG0480022307.01.T01:CDS
TCTTGTTGATTCGCTTTGTT+AGG 0.272796 4:+70967093 MsG0480022307.01.T02:CDS
TCACGGAGAGCGACGGTTCC+TGG 0.319730 4:-70966811 None:intergenic
TGAGGAGCTCAGTGCTCTTC+TGG 0.339681 4:-70966846 None:intergenic
GTGCCGACACGGCGGAGCTC+TGG 0.383446 4:-70966936 None:intergenic
TTCTTCACTCCGCCGGTGGC+CGG 0.393192 4:-70966772 None:intergenic
TTTGTGGCGAGTTGCTTCCT+TGG 0.400731 4:-70966730 None:intergenic
GGAAGGGGAGTTTGCGGATG+AGG 0.429699 4:-70966864 None:intergenic
TTATCTCGTCGGTCTCTTTG+AGG 0.447224 4:+70966978 MsG0480022307.01.T01:CDS
CTTCCTTGGAGCTTTGCCAC+CGG 0.456245 4:-70966716 None:intergenic
CGCAAATCTGCTCCGGCCAC+CGG 0.457456 4:+70966760 MsG0480022307.01.T01:CDS
CGCTTCTTGCAGTGCCGACA+CGG 0.458211 4:-70966947 None:intergenic
CTTGTTGATTCGCTTTGTTA+GGG 0.468737 4:+70967094 MsG0480022307.01.T02:CDS
AGTGACACGCTTAGCATGAA+TGG 0.474744 4:-70967016 None:intergenic
CCTTGGAGCTTTGCCACCGG+TGG 0.488901 4:-70966713 None:intergenic
GGCACTGCAAGAAGCGGCCG+AGG 0.489550 4:+70966954 MsG0480022307.01.T01:CDS
CGACGGTTCCTGGCCTGAAA+CGG 0.503357 4:-70966801 None:intergenic
GGATTTGCGAGCGGTTTGCT+TGG 0.506530 4:-70966692 None:intergenic
CTAGCGAGCTGGATATCCTT+AGG 0.510191 4:-70967045 None:intergenic
AAACCTCACCGTTTCAGGCC+AGG 0.512079 4:+70966793 MsG0480022307.01.T01:CDS
CACGGATACGCCTAGCGAGC+TGG 0.513784 4:-70967056 None:intergenic
GACGCTGGAAGGGGAGTTTG+CGG 0.521996 4:-70966870 None:intergenic
TTTCCAGAGCTCCGCCGTGT+CGG 0.522343 4:+70966933 MsG0480022307.01.T01:CDS
ACACGGCGGAGCTCTGGAAA+CGG 0.525377 4:-70966930 None:intergenic
AGCCGCTCGCAAATCTGCTC+CGG 0.526992 4:+70966753 MsG0480022307.01.T01:CDS
CACGGACAAGACGCTGGAAG+GGG 0.529109 4:-70966879 None:intergenic
CTCACGGACAAGACGCTGGA+AGG 0.539901 4:-70966881 None:intergenic
GTGAGGTTTCTTCACTCCGC+CGG 0.553823 4:-70966779 None:intergenic
CTAAGGATATCCAGCTCGCT+AGG 0.554905 4:+70967046 MsG0480022307.01.T01:CDS
GCGTGTCACTATCATGCCTA+AGG 0.557496 4:+70967029 MsG0480022307.01.T01:CDS
TGAAATCCTGAGCGATCTCA+CGG 0.570370 4:-70966897 None:intergenic
TCTGGTACTTGCGGATCTCA+CGG 0.571857 4:-70966828 None:intergenic
CCACCGGTGGCAAAGCTCCA+AGG 0.572773 4:+70966713 MsG0480022307.01.T01:CDS
CAGTGCTCTTCTGGTACTTG+CGG 0.579006 4:-70966837 None:intergenic
TCACGGACAAGACGCTGGAA+GGG 0.583031 4:-70966880 None:intergenic
AAATCTGCTCCGGCCACCGG+CGG 0.584127 4:+70966763 MsG0480022307.01.T01:CDS
CGATCTCACGGACAAGACGC+TGG 0.585935 4:-70966885 None:intergenic
AGGTTTCTTCACTCCGCCGG+TGG 0.590490 4:-70966776 None:intergenic
CGTGTCGGCACTGCAAGAAG+CGG 0.596423 4:+70966948 MsG0480022307.01.T01:CDS
TCTTGTCCGTGAGATCGCTC+AGG 0.603921 4:+70966891 MsG0480022307.01.T01:CDS
GCGGCCGAGGCTTATCTCGT+CGG 0.608232 4:+70966967 MsG0480022307.01.T01:CDS
CAAACCGCTCGCAAATCCAC+CGG 0.613586 4:+70966697 MsG0480022307.01.T01:CDS
ACCGCTCGCAAATCCACCGG+TGG 0.632751 4:+70966700 MsG0480022307.01.T01:CDS
AAGATCAAGCACGCTCGCCA+CGG 0.639365 4:-70967074 None:intergenic
GGCCGGAGCAGATTTGCGAG+CGG 0.640066 4:-70966755 None:intergenic
GCCACCGGTGGATTTGCGAG+CGG 0.642552 4:-70966701 None:intergenic
GAGACCGACGAGATAAGCCT+CGG 0.653052 4:-70966971 None:intergenic
GCGGATCTCACGGAGAGCGA+CGG 0.662183 4:-70966818 None:intergenic
GTTCCTGGCCTGAAACGGTG+AGG 0.663103 4:-70966796 None:intergenic
CAGCTCGCTAGGCGTATCCG+TGG 0.667707 4:+70967057 MsG0480022307.01.T01:CDS
GCTTAGCATGAATGGCGCAG+AGG 0.694649 4:-70967008 None:intergenic
TTCTTGCAGTGCCGACACGG+CGG 0.739278 4:-70966944 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGTGACACGCTTAGCATGAA+TGG - Chr4:70967019-70967038 None:intergenic 45.0%
TGAAATCCTGAGCGATCTCA+CGG - Chr4:70966900-70966919 None:intergenic 45.0%
TGAAGAAACCTCACCGTTTC+AGG + Chr4:70966788-70966807 MsG0480022307.01.T01:CDS 45.0%
TTATCTCGTCGGTCTCTTTG+AGG + Chr4:70966978-70966997 MsG0480022307.01.T01:CDS 45.0%
CAGTGCTCTTCTGGTACTTG+CGG - Chr4:70966840-70966859 None:intergenic 50.0%
CTAAGGATATCCAGCTCGCT+AGG + Chr4:70967046-70967065 MsG0480022307.01.T01:CDS 50.0%
CTAGCGAGCTGGATATCCTT+AGG - Chr4:70967048-70967067 None:intergenic 50.0%
TCTGGTACTTGCGGATCTCA+CGG - Chr4:70966831-70966850 None:intergenic 50.0%
TTTGTGGCGAGTTGCTTCCT+TGG - Chr4:70966733-70966752 None:intergenic 50.0%
! AGATTTGCGAGCGGCTTTTG+TGG - Chr4:70966749-70966768 None:intergenic 50.0%
! GCGTGTCACTATCATGCCTA+AGG + Chr4:70967029-70967048 MsG0480022307.01.T01:CDS 50.0%
AAACCTCACCGTTTCAGGCC+AGG + Chr4:70966793-70966812 MsG0480022307.01.T01:CDS 55.0%
AAGATCAAGCACGCTCGCCA+CGG - Chr4:70967077-70967096 None:intergenic 55.0%
CAAACCGCTCGCAAATCCAC+CGG + Chr4:70966697-70966716 MsG0480022307.01.T01:CDS 55.0%
CTTCCTTGGAGCTTTGCCAC+CGG - Chr4:70966719-70966738 None:intergenic 55.0%
GAGACCGACGAGATAAGCCT+CGG - Chr4:70966974-70966993 None:intergenic 55.0%
GCTTAGCATGAATGGCGCAG+AGG - Chr4:70967011-70967030 None:intergenic 55.0%
GTGAGGTTTCTTCACTCCGC+CGG - Chr4:70966782-70966801 None:intergenic 55.0%
TCACGGACAAGACGCTGGAA+GGG - Chr4:70966883-70966902 None:intergenic 55.0%
TCTTGTCCGTGAGATCGCTC+AGG + Chr4:70966891-70966910 MsG0480022307.01.T01:CDS 55.0%
TGAGGAGCTCAGTGCTCTTC+TGG - Chr4:70966849-70966868 None:intergenic 55.0%
! GGATTTGCGAGCGGTTTGCT+TGG - Chr4:70966695-70966714 None:intergenic 55.0%
AGCCGCTCGCAAATCTGCTC+CGG + Chr4:70966753-70966772 MsG0480022307.01.T01:CDS 60.0%
AGGTTTCTTCACTCCGCCGG+TGG - Chr4:70966779-70966798 None:intergenic 60.0%
CACGGACAAGACGCTGGAAG+GGG - Chr4:70966882-70966901 None:intergenic 60.0%
CGACGGTTCCTGGCCTGAAA+CGG - Chr4:70966804-70966823 None:intergenic 60.0%
CGATCTCACGGACAAGACGC+TGG - Chr4:70966888-70966907 None:intergenic 60.0%
CTCACGGACAAGACGCTGGA+AGG - Chr4:70966884-70966903 None:intergenic 60.0%
GACGCTGGAAGGGGAGTTTG+CGG - Chr4:70966873-70966892 None:intergenic 60.0%
GGAAGGGGAGTTTGCGGATG+AGG - Chr4:70966867-70966886 None:intergenic 60.0%
GTTCCTGGCCTGAAACGGTG+AGG - Chr4:70966799-70966818 None:intergenic 60.0%
TTTCCAGAGCTCCGCCGTGT+CGG + Chr4:70966933-70966952 MsG0480022307.01.T01:CDS 60.0%
! CGTGTCGGCACTGCAAGAAG+CGG + Chr4:70966948-70966967 MsG0480022307.01.T01:CDS 60.0%
!! ACACGGCGGAGCTCTGGAAA+CGG - Chr4:70966933-70966952 None:intergenic 60.0%
!! CGCTTCTTGCAGTGCCGACA+CGG - Chr4:70966950-70966969 None:intergenic 60.0%
!! TTCTTGCAGTGCCGACACGG+CGG - Chr4:70966947-70966966 None:intergenic 60.0%
AAATCTGCTCCGGCCACCGG+CGG + Chr4:70966763-70966782 MsG0480022307.01.T01:CDS 65.0%
ACCGCTCGCAAATCCACCGG+TGG + Chr4:70966700-70966719 MsG0480022307.01.T01:CDS 65.0%
CACGGATACGCCTAGCGAGC+TGG - Chr4:70967059-70967078 None:intergenic 65.0%
CAGCTCGCTAGGCGTATCCG+TGG + Chr4:70967057-70967076 MsG0480022307.01.T01:CDS 65.0%
CCACCGGTGGCAAAGCTCCA+AGG + Chr4:70966713-70966732 MsG0480022307.01.T01:CDS 65.0%
CCTTGGAGCTTTGCCACCGG+TGG - Chr4:70966716-70966735 None:intergenic 65.0%
CGCAAATCTGCTCCGGCCAC+CGG + Chr4:70966760-70966779 MsG0480022307.01.T01:CDS 65.0%
GCGGATCTCACGGAGAGCGA+CGG - Chr4:70966821-70966840 None:intergenic 65.0%
GCGGCCGAGGCTTATCTCGT+CGG + Chr4:70966967-70966986 MsG0480022307.01.T01:CDS 65.0%
GGCCGGAGCAGATTTGCGAG+CGG - Chr4:70966758-70966777 None:intergenic 65.0%
TCACGGAGAGCGACGGTTCC+TGG - Chr4:70966814-70966833 None:intergenic 65.0%
TTCTTCACTCCGCCGGTGGC+CGG - Chr4:70966775-70966794 None:intergenic 65.0%
! GCCACCGGTGGATTTGCGAG+CGG - Chr4:70966704-70966723 None:intergenic 65.0%
GGCACTGCAAGAAGCGGCCG+AGG + Chr4:70966954-70966973 MsG0480022307.01.T01:CDS 70.0%
! GTGCCGACACGGCGGAGCTC+TGG - Chr4:70966939-70966958 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70966682 70967114 70966682 ID=MsG0480022307.01;Name=MsG0480022307.01
Chr4 mRNA 70966682 70967092 70966682 ID=MsG0480022307.01.T01;Parent=MsG0480022307.01;Name=MsG0480022307.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|136
Chr4 exon 70966682 70967092 70966682 ID=MsG0480022307.01.T01:exon:2356;Parent=MsG0480022307.01.T01
Chr4 CDS 70966682 70967092 70966682 ID=MsG0480022307.01.T01:cds;Parent=MsG0480022307.01.T01
Chr4 mRNA 70967022 70967114 70967022 ID=MsG0480022307.01.T02;Parent=MsG0480022307.01;Name=MsG0480022307.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|30
Chr4 exon 70967022 70967114 70967022 ID=MsG0480022307.01.T02:exon:2357;Parent=MsG0480022307.01.T02
Chr4 CDS 70967022 70967114 70967022 ID=MsG0480022307.01.T02:cds;Parent=MsG0480022307.01.T02
Gene Sequence

>MsG0480022307.01.T01

ATGGCACGTACCAAGCAAACCGCTCGCAAATCCACCGGTGGCAAAGCTCCAAGGAAGCAACTCGCCACAAAAGCCGCTCGCAAATCTGCTCCGGCCACCGGCGGAGTGAAGAAACCTCACCGTTTCAGGCCAGGAACCGTCGCTCTCCGTGAGATCCGCAAGTACCAGAAGAGCACTGAGCTCCTCATCCGCAAACTCCCCTTCCAGCGTCTTGTCCGTGAGATCGCTCAGGATTTCAAGACTGATCTCCGTTTCCAGAGCTCCGCCGTGTCGGCACTGCAAGAAGCGGCCGAGGCTTATCTCGTCGGTCTCTTTGAGGATACTAACCTCTGCGCCATTCATGCTAAGCGTGTCACTATCATGCCTAAGGATATCCAGCTCGCTAGGCGTATCCGTGGCGAGCGTGCTTGA

>MsG0480022307.01.T02

ATGCTAAGCGTGTCACTATCATGCCTAAGGATATCCAGCTCGCTAGGCGTATCCGTGGCGAGCGTGCTTGATCTTGTTGATTCGCTTTGTTAG

Protein sequence

>MsG0480022307.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*

>MsG0480022307.01.T02

MLSVSLSCLRISSSLGVSVASVLDLVDSLC*