AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023842.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023842.01.T01 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0480023842.01.T01 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.15e-95 275
MsG0480023842.01.T01 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0480023842.01.T01 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0480023842.01.T01 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0480023842.01.T01 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.44e-92 264
MsG0480023842.01.T01 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.38e-91 262
MsG0480023842.01.T01 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0480023842.01.T01 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0480023842.01.T01 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.82e-82 238
MsG0480023842.01.T01 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.64e-75 219
MsG0480023842.01.T01 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.05e-65 195
MsG0480023842.01.T01 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.74e-56 171
MsG0480023842.01.T01 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.96e-52 160
MsG0480023842.01.T01 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.69e-45 144
MsG0480023842.01.T01 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.67e-43 139
MsG0480023842.01.T01 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.74e-42 137
MsG0480023842.01.T01 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.50e-40 132
MsG0480023842.01.T01 MTR_8g027840 55.102 98 43 1 37 133 28 125 5.01e-30 105
MsG0480023842.01.T01 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.48e-25 92.4
MsG0480023842.01.T01 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.75e-20 80.9
MsG0480023842.01.T01 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.26e-18 73.6
MsG0480023842.01.T01 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.81e-15 66.2
MsG0480023842.01.T01 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.48e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023842.01.T01 AT5G65360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480023842.01.T01 AT5G10390 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480023842.01.T01 AT5G10400 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480023842.01.T01 AT3G27360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480023842.01.T01 AT1G09200 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0480023842.01.T01 AT5G10980 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480023842.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480023842.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480023842.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480023842.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480023842.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480023842.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0480023842.01.T01 AT4G40030 97.059 136 4 0 1 136 29 164 7.04e-93 266
MsG0480023842.01.T01 AT5G65350 94.853 136 7 0 1 136 1 136 5.30e-91 260
MsG0480023842.01.T01 AT1G75600 93.382 136 9 0 1 136 1 136 1.25e-89 256
MsG0480023842.01.T01 AT1G13370 93.382 136 9 0 1 136 1 136 5.55e-89 254
MsG0480023842.01.T01 AT1G19890 89.781 137 13 1 1 136 1 137 1.07e-83 241
MsG0480023842.01.T01 AT5G12910 71.111 135 34 2 1 135 1 130 9.89e-67 198
MsG0480023842.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0480023842.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0480023842.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0480023842.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 41 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTCTCTAACCAATCTT+TGG 0.224743 4:+90393669 None:intergenic
GCCGCTGAAGCTTATCTTGT+TGG 0.348800 4:-90393587 MsG0480023842.01.T01:CDS
TTGAATATCCTTAGGCATAA+TGG 0.349539 4:+90393517 None:intergenic
AGCTGATTTCCGAGCGGCTT+TGG 0.356003 4:+90393805 None:intergenic
TCACGAAGAGCAACAGTTCC+GGG 0.371586 4:+90393743 None:intergenic
CTCACGAAGAGCAACAGTTC+CGG 0.390727 4:+90393742 None:intergenic
TTCTTCACTCCTCCGGTGGC+CGG 0.400280 4:+90393782 None:intergenic
GAAGCTTCCATTCCAAAGAT+TGG 0.412914 4:-90393681 MsG0480023842.01.T01:CDS
CTTGCAAGTTGAATATCCTT+AGG 0.413826 4:+90393509 None:intergenic
AGGATTTCAAAACTGATCTC+AGG 0.429009 4:-90393643 MsG0480023842.01.T01:CDS
GGATTTGCGAGCGGTTTGTT+TGG 0.441284 4:+90393862 None:intergenic
AGAGCACTGAGCTTCTCATC+AGG 0.458709 4:-90393703 MsG0480023842.01.T01:CDS
TTGGTGGCGAGTTGTTTCCT+TGG 0.473034 4:+90393824 None:intergenic
GGCTTCTTGAAGAGCAGAAA+CGG 0.474881 4:+90393607 None:intergenic
TTTCCTTGGAGCTTTGCCTC+CGG 0.476973 4:+90393838 None:intergenic
CGGAAATCAGCTCCGGCCAC+CGG 0.487022 4:-90393794 MsG0480023842.01.T01:CDS
CCTTGGAGCTTTGCCTCCGG+TGG 0.489182 4:+90393841 None:intergenic
TGATTTCCGAGCGGCTTTGG+TGG 0.495094 4:+90393808 None:intergenic
AGCCGCTCGGAAATCAGCTC+CGG 0.498921 4:-90393801 MsG0480023842.01.T01:CDS
CAGTGCTCTTCTGATACTTA+CGG 0.501233 4:+90393717 None:intergenic
GTGTGGCTTCTTCACTCCTC+CGG 0.509508 4:+90393775 None:intergenic
TCTCTAACCAATCTTTGGAA+TGG 0.520211 4:+90393674 None:intergenic
GAGAGTTACCATTATGCCTA+AGG 0.535535 4:-90393525 MsG0480023842.01.T01:CDS
AACTCGCCACCAAAGCCGCT+CGG 0.570459 4:-90393814 MsG0480023842.01.T01:CDS
CCACCGGAGGCAAAGCTCCA+AGG 0.572363 4:-90393841 MsG0480023842.01.T01:CDS
AAGAAGAATCAGAGGCGAGA+GGG 0.597241 4:-90393489 MsG0480023842.01.T01:CDS
TGGCTTCTTCACTCCTCCGG+TGG 0.598450 4:+90393778 None:intergenic
AAATCAGCTCCGGCCACCGG+AGG 0.599225 4:-90393791 MsG0480023842.01.T01:CDS
ATTGGTTAGAGAAATCGCTC+AGG 0.600201 4:-90393663 MsG0480023842.01.T01:CDS
AGCATGAATAGCGCAAAGGT+TGG 0.613099 4:+90393550 None:intergenic
CAAACCGCTCGCAAATCCAC+CGG 0.629023 4:-90393857 MsG0480023842.01.T01:CDS
GCCTCCGGTGGATTTGCGAG+CGG 0.639769 4:+90393853 None:intergenic
ACCGCTCGCAAATCCACCGG+AGG 0.648108 4:-90393854 MsG0480023842.01.T01:CDS
CAAGAAGAATCAGAGGCGAG+AGG 0.648850 4:-90393490 MsG0480023842.01.T01:CDS
AAGCCACACAGATTCCGTCC+CGG 0.653763 4:-90393761 MsG0480023842.01.T01:CDS
TCTTAGCATGAATAGCGCAA+AGG 0.658330 4:+90393546 None:intergenic
CAACTTGCAAGAAGAATCAG+AGG 0.660238 4:-90393497 MsG0480023842.01.T01:CDS
ACCAACAAGATAAGCTTCAG+CGG 0.667251 4:+90393586 None:intergenic
GAAGAGCAACAGTTCCGGGA+CGG 0.670696 4:+90393747 None:intergenic
GTTCCGGGACGGAATCTGTG+TGG 0.693886 4:+90393758 None:intergenic
GGCCGGAGCTGATTTCCGAG+CGG 0.718116 4:+90393799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGAATATCCTTAGGCATAA+TGG + Chr4:90393842-90393861 None:intergenic 30.0%
AGGATTTCAAAACTGATCTC+AGG - Chr4:90393713-90393732 MsG0480023842.01.T01:CDS 35.0%
CGATTTCTCTAACCAATCTT+TGG + Chr4:90393690-90393709 None:intergenic 35.0%
! CTTGCAAGTTGAATATCCTT+AGG + Chr4:90393850-90393869 None:intergenic 35.0%
! TCTCTAACCAATCTTTGGAA+TGG + Chr4:90393685-90393704 None:intergenic 35.0%
ACCAACAAGATAAGCTTCAG+CGG + Chr4:90393773-90393792 None:intergenic 40.0%
ATTGGTTAGAGAAATCGCTC+AGG - Chr4:90393693-90393712 MsG0480023842.01.T01:CDS 40.0%
CAACTTGCAAGAAGAATCAG+AGG - Chr4:90393859-90393878 MsG0480023842.01.T01:CDS 40.0%
CAGTGCTCTTCTGATACTTA+CGG + Chr4:90393642-90393661 None:intergenic 40.0%
GAAGCTTCCATTCCAAAGAT+TGG - Chr4:90393675-90393694 MsG0480023842.01.T01:CDS 40.0%
GAGAGTTACCATTATGCCTA+AGG - Chr4:90393831-90393850 MsG0480023842.01.T01:CDS 40.0%
TCTTAGCATGAATAGCGCAA+AGG + Chr4:90393813-90393832 None:intergenic 40.0%
AAGAAGAATCAGAGGCGAGA+GGG - Chr4:90393867-90393886 MsG0480023842.01.T01:CDS 45.0%
AGCATGAATAGCGCAAAGGT+TGG + Chr4:90393809-90393828 None:intergenic 45.0%
GGCTTCTTGAAGAGCAGAAA+CGG + Chr4:90393752-90393771 None:intergenic 45.0%
CAAGAAGAATCAGAGGCGAG+AGG - Chr4:90393866-90393885 MsG0480023842.01.T01:CDS 50.0%
CTCACGAAGAGCAACAGTTC+CGG + Chr4:90393617-90393636 None:intergenic 50.0%
GCCGCTGAAGCTTATCTTGT+TGG - Chr4:90393769-90393788 MsG0480023842.01.T01:CDS 50.0%
TCACGAAGAGCAACAGTTCC+GGG + Chr4:90393616-90393635 None:intergenic 50.0%
TTTCCTTGGAGCTTTGCCTC+CGG + Chr4:90393521-90393540 None:intergenic 50.0%
! GGATTTGCGAGCGGTTTGTT+TGG + Chr4:90393497-90393516 None:intergenic 50.0%
!! AGAGCACTGAGCTTCTCATC+AGG - Chr4:90393653-90393672 MsG0480023842.01.T01:CDS 50.0%
!! TTGGTGGCGAGTTGTTTCCT+TGG + Chr4:90393535-90393554 None:intergenic 50.0%
AAGCCACACAGATTCCGTCC+CGG - Chr4:90393595-90393614 MsG0480023842.01.T01:CDS 55.0%
CAAACCGCTCGCAAATCCAC+CGG - Chr4:90393499-90393518 MsG0480023842.01.T01:CDS 55.0%
GAAGAGCAACAGTTCCGGGA+CGG + Chr4:90393612-90393631 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + Chr4:90393584-90393603 None:intergenic 55.0%
! AGCTGATTTCCGAGCGGCTT+TGG + Chr4:90393554-90393573 None:intergenic 55.0%
! TGATTTCCGAGCGGCTTTGG+TGG + Chr4:90393551-90393570 None:intergenic 55.0%
AACTCGCCACCAAAGCCGCT+CGG - Chr4:90393542-90393561 MsG0480023842.01.T01:CDS 60.0%
AGCCGCTCGGAAATCAGCTC+CGG - Chr4:90393555-90393574 MsG0480023842.01.T01:CDS 60.0%
GTTCCGGGACGGAATCTGTG+TGG + Chr4:90393601-90393620 None:intergenic 60.0%
TGGCTTCTTCACTCCTCCGG+TGG + Chr4:90393581-90393600 None:intergenic 60.0%
TTCTTCACTCCTCCGGTGGC+CGG + Chr4:90393577-90393596 None:intergenic 60.0%
AAATCAGCTCCGGCCACCGG+AGG - Chr4:90393565-90393584 MsG0480023842.01.T01:CDS 65.0%
ACCGCTCGCAAATCCACCGG+AGG - Chr4:90393502-90393521 MsG0480023842.01.T01:CDS 65.0%
CCACCGGAGGCAAAGCTCCA+AGG - Chr4:90393515-90393534 MsG0480023842.01.T01:CDS 65.0%
CCTTGGAGCTTTGCCTCCGG+TGG + Chr4:90393518-90393537 None:intergenic 65.0%
CGGAAATCAGCTCCGGCCAC+CGG - Chr4:90393562-90393581 MsG0480023842.01.T01:CDS 65.0%
! GCCTCCGGTGGATTTGCGAG+CGG + Chr4:90393506-90393525 None:intergenic 65.0%
! GGCCGGAGCTGATTTCCGAG+CGG + Chr4:90393560-90393579 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 90393484 90393894 90393484 ID=MsG0480023842.01;Name=MsG0480023842.01
Chr4 mRNA 90393484 90393894 90393484 ID=MsG0480023842.01.T01;Parent=MsG0480023842.01;Name=MsG0480023842.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|136
Chr4 exon 90393484 90393894 90393484 ID=MsG0480023842.01.T01:exon:7594;Parent=MsG0480023842.01.T01
Chr4 CDS 90393484 90393894 90393484 ID=MsG0480023842.01.T01:cds;Parent=MsG0480023842.01.T01
Gene Sequence

>MsG0480023842.01.T01

ATGGCTCGTACCAAACAAACCGCTCGCAAATCCACCGGAGGCAAAGCTCCAAGGAAACAACTCGCCACCAAAGCCGCTCGGAAATCAGCTCCGGCCACCGGAGGAGTGAAGAAGCCACACAGATTCCGTCCCGGAACTGTTGCTCTTCGTGAGATCCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCAGGAAGCTTCCATTCCAAAGATTGGTTAGAGAAATCGCTCAGGATTTCAAAACTGATCTCAGGTTTCAGAGTAGCGCCGTTTCTGCTCTTCAAGAAGCCGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACCAACCTTTGCGCTATTCATGCTAAGAGAGTTACCATTATGCCTAAGGATATTCAACTTGCAAGAAGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>MsG0480023842.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*