AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025504.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025504.01.T01 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0580025504.01.T01 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.15e-95 275
MsG0580025504.01.T01 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0580025504.01.T01 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0580025504.01.T01 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0580025504.01.T01 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.44e-92 264
MsG0580025504.01.T01 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.38e-91 262
MsG0580025504.01.T01 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0580025504.01.T01 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0580025504.01.T01 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.82e-82 238
MsG0580025504.01.T01 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.64e-75 219
MsG0580025504.01.T01 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.05e-65 195
MsG0580025504.01.T01 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.74e-56 171
MsG0580025504.01.T01 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.96e-52 160
MsG0580025504.01.T01 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.69e-45 144
MsG0580025504.01.T01 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.67e-43 139
MsG0580025504.01.T01 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.74e-42 137
MsG0580025504.01.T01 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.50e-40 132
MsG0580025504.01.T01 MTR_8g027840 55.102 98 43 1 37 133 28 125 5.01e-30 105
MsG0580025504.01.T01 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.48e-25 92.4
MsG0580025504.01.T01 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.75e-20 80.9
MsG0580025504.01.T01 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.26e-18 73.6
MsG0580025504.01.T01 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.81e-15 66.2
MsG0580025504.01.T01 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.48e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025504.01.T01 AT5G65360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025504.01.T01 AT5G10390 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025504.01.T01 AT5G10400 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025504.01.T01 AT3G27360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025504.01.T01 AT1G09200 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0580025504.01.T01 AT5G10980 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025504.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025504.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025504.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025504.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025504.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025504.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0580025504.01.T01 AT4G40030 97.059 136 4 0 1 136 29 164 7.04e-93 266
MsG0580025504.01.T01 AT5G65350 94.853 136 7 0 1 136 1 136 5.30e-91 260
MsG0580025504.01.T01 AT1G75600 93.382 136 9 0 1 136 1 136 1.25e-89 256
MsG0580025504.01.T01 AT1G13370 93.382 136 9 0 1 136 1 136 5.55e-89 254
MsG0580025504.01.T01 AT1G19890 89.781 137 13 1 1 136 1 137 1.07e-83 241
MsG0580025504.01.T01 AT5G12910 71.111 135 34 2 1 135 1 130 9.89e-67 198
MsG0580025504.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0580025504.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0580025504.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0580025504.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 37 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTTGCCGGAGCAGATTTC+CGG 0.243304 5:-19703284 None:intergenic
TTGGTGGCTAGCTGCTTTCT+TGG 0.277587 5:-19703255 None:intergenic
TTCTTGACTCCTCCGGTTGC+CGG 0.293014 5:-19703297 None:intergenic
TCAGAAGCTCAGTGCTCTTC+TGG 0.307424 5:-19703371 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 5:+19703492 MsG0580025504.01.T01:CDS
GTGCAGAAACAGCGCTGCTT+TGG 0.363708 5:-19703461 None:intergenic
TCACGAAGTGCAACAGTTCC+AGG 0.377831 5:-19703336 None:intergenic
AGCAGATTTCCGGGCAGCTT+TGG 0.390197 5:-19703274 None:intergenic
CGGAAATCTGCTCCGGCAAC+CGG 0.392471 5:+19703285 MsG0580025504.01.T01:CDS
GGTTGCCGGAGCAGATTTCC+GGG 0.407337 5:-19703283 None:intergenic
CTTTCTTGGTGCCTTTCCTC+CGG 0.425692 5:-19703241 None:intergenic
TTATCTTGTTGGTTTGTTTG+AGG 0.428809 5:+19703503 MsG0580025504.01.T01:CDS
CGATTTCACGAACAAGTCTC+TGG 0.432729 5:-19703410 None:intergenic
AGATTTCCGGGCAGCTTTGG+TGG 0.468887 5:-19703271 None:intergenic
AAACAGCGCTGCTTTGGAAT+CGG 0.473836 5:-19703455 None:intergenic
AGCTAGCCACCAAAGCTGCC+CGG 0.491500 5:+19703265 MsG0580025504.01.T01:CDS
CGAGCGAGTTGAATATCCTT+AGG 0.499677 5:-19703570 None:intergenic
GTGTGGCTTCTTGACTCCTC+CGG 0.501667 5:-19703304 None:intergenic
AGCTGCCCGGAAATCTGCTC+CGG 0.505877 5:+19703278 MsG0580025504.01.T01:CDS
AGTAACTCTCTTAGCATGAA+TGG 0.521941 5:-19703541 None:intergenic
AGAGCACTGAGCTTCTGATA+AGG 0.531497 5:+19703376 MsG0580025504.01.T01:CDS
TCGCAAATCCACCGGAGGAA+AGG 0.532804 5:+19703230 MsG0580025504.01.T01:CDS
TGTTTGCTTTGTACGAGCCA+TGG 0.537743 5:-19703205 None:intergenic
GAGAGTTACTATCATGCCTA+AGG 0.550012 5:+19703554 MsG0580025504.01.T01:CDS
ACTTGTTCGTGAAATCGCTC+AGG 0.551065 5:+19703416 MsG0580025504.01.T01:CDS
CAACTCGCTCGCAGAATCAG+AGG 0.556058 5:+19703582 MsG0580025504.01.T01:CDS
TCTTGGTGCCTTTCCTCCGG+TGG 0.556160 5:-19703238 None:intergenic
CAGTGCTCTTCTGGTACTTG+CGG 0.579006 5:-19703362 None:intergenic
TCGCAGAATCAGAGGCGAGA+GGG 0.584947 5:+19703590 MsG0580025504.01.T01:CDS
CAAACAGCTCGCAAATCCAC+CGG 0.586123 5:+19703222 MsG0580025504.01.T01:CDS
TCACGAACAAGTCTCTGGAA+TGG 0.590006 5:-19703405 None:intergenic
CTCGCAGAATCAGAGGCGAG+AGG 0.599896 5:+19703589 MsG0580025504.01.T01:CDS
AAATCTGCTCCGGCAACCGG+AGG 0.607496 5:+19703288 MsG0580025504.01.T01:CDS
AAGCCACACAGATTCCGTCC+TGG 0.622196 5:+19703318 MsG0580025504.01.T01:CDS
GAAGTGCAACAGTTCCAGGA+CGG 0.690156 5:-19703332 None:intergenic
GTTCCAGGACGGAATCTGTG+TGG 0.699556 5:-19703321 None:intergenic
ACAGCTCGCAAATCCACCGG+AGG 0.704783 5:+19703225 MsG0580025504.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATCTTGTTGGTTTGTTTG+AGG + Chr5:19703503-19703522 MsG0580025504.01.T01:CDS 30.0%
AGTAACTCTCTTAGCATGAA+TGG - Chr5:19703544-19703563 None:intergenic 35.0%
GAGAGTTACTATCATGCCTA+AGG + Chr5:19703554-19703573 MsG0580025504.01.T01:CDS 40.0%
ACTTGTTCGTGAAATCGCTC+AGG + Chr5:19703416-19703435 MsG0580025504.01.T01:CDS 45.0%
CGATTTCACGAACAAGTCTC+TGG - Chr5:19703413-19703432 None:intergenic 45.0%
GCTGCTGAAGCTTATCTTGT+TGG + Chr5:19703492-19703511 MsG0580025504.01.T01:CDS 45.0%
! CGAGCGAGTTGAATATCCTT+AGG - Chr5:19703573-19703592 None:intergenic 45.0%
! TCACGAACAAGTCTCTGGAA+TGG - Chr5:19703408-19703427 None:intergenic 45.0%
!! AAACAGCGCTGCTTTGGAAT+CGG - Chr5:19703458-19703477 None:intergenic 45.0%
!! AGAGCACTGAGCTTCTGATA+AGG + Chr5:19703376-19703395 MsG0580025504.01.T01:CDS 45.0%
CAAACAGCTCGCAAATCCAC+CGG + Chr5:19703222-19703241 MsG0580025504.01.T01:CDS 50.0%
CAGTGCTCTTCTGGTACTTG+CGG - Chr5:19703365-19703384 None:intergenic 50.0%
GAAGTGCAACAGTTCCAGGA+CGG - Chr5:19703335-19703354 None:intergenic 50.0%
TCACGAAGTGCAACAGTTCC+AGG - Chr5:19703339-19703358 None:intergenic 50.0%
TCAGAAGCTCAGTGCTCTTC+TGG - Chr5:19703374-19703393 None:intergenic 50.0%
! TTGGTGGCTAGCTGCTTTCT+TGG - Chr5:19703258-19703277 None:intergenic 50.0%
!! CTTTCTTGGTGCCTTTCCTC+CGG - Chr5:19703244-19703263 None:intergenic 50.0%
AAGCCACACAGATTCCGTCC+TGG + Chr5:19703318-19703337 MsG0580025504.01.T01:CDS 55.0%
AGCAGATTTCCGGGCAGCTT+TGG - Chr5:19703277-19703296 None:intergenic 55.0%
CAACTCGCTCGCAGAATCAG+AGG + Chr5:19703582-19703601 MsG0580025504.01.T01:CDS 55.0%
GTGCAGAAACAGCGCTGCTT+TGG - Chr5:19703464-19703483 None:intergenic 55.0%
GTGTGGCTTCTTGACTCCTC+CGG - Chr5:19703307-19703326 None:intergenic 55.0%
GTTCCAGGACGGAATCTGTG+TGG - Chr5:19703324-19703343 None:intergenic 55.0%
TCGCAAATCCACCGGAGGAA+AGG + Chr5:19703230-19703249 MsG0580025504.01.T01:CDS 55.0%
TCGCAGAATCAGAGGCGAGA+GGG + Chr5:19703590-19703609 MsG0580025504.01.T01:CDS 55.0%
TTCTTGACTCCTCCGGTTGC+CGG - Chr5:19703300-19703319 None:intergenic 55.0%
! AGATTTCCGGGCAGCTTTGG+TGG - Chr5:19703274-19703293 None:intergenic 55.0%
AAATCTGCTCCGGCAACCGG+AGG + Chr5:19703288-19703307 MsG0580025504.01.T01:CDS 60.0%
ACAGCTCGCAAATCCACCGG+AGG + Chr5:19703225-19703244 MsG0580025504.01.T01:CDS 60.0%
AGCTAGCCACCAAAGCTGCC+CGG + Chr5:19703265-19703284 MsG0580025504.01.T01:CDS 60.0%
AGCTGCCCGGAAATCTGCTC+CGG + Chr5:19703278-19703297 MsG0580025504.01.T01:CDS 60.0%
CGGAAATCTGCTCCGGCAAC+CGG + Chr5:19703285-19703304 MsG0580025504.01.T01:CDS 60.0%
CGGTTGCCGGAGCAGATTTC+CGG - Chr5:19703287-19703306 None:intergenic 60.0%
CTCGCAGAATCAGAGGCGAG+AGG + Chr5:19703589-19703608 MsG0580025504.01.T01:CDS 60.0%
GGTTGCCGGAGCAGATTTCC+GGG - Chr5:19703286-19703305 None:intergenic 60.0%
!! TCTTGGTGCCTTTCCTCCGG+TGG - Chr5:19703241-19703260 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 19703207 19703617 19703207 ID=MsG0580025504.01;Name=MsG0580025504.01
Chr5 mRNA 19703207 19703617 19703207 ID=MsG0580025504.01.T01;Parent=MsG0580025504.01;Name=MsG0580025504.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|136
Chr5 exon 19703207 19703617 19703207 ID=MsG0580025504.01.T01:exon:8300;Parent=MsG0580025504.01.T01
Chr5 CDS 19703207 19703617 19703207 ID=MsG0580025504.01.T01:cds;Parent=MsG0580025504.01.T01
Gene Sequence

>MsG0580025504.01.T01

ATGGCTCGTACAAAGCAAACAGCTCGCAAATCCACCGGAGGAAAGGCACCAAGAAAGCAGCTAGCCACCAAAGCTGCCCGGAAATCTGCTCCGGCAACCGGAGGAGTCAAGAAGCCACACAGATTCCGTCCTGGAACTGTTGCACTTCGTGAGATCCGCAAGTACCAGAAGAGCACTGAGCTTCTGATAAGGAAGCTTCCATTCCAGAGACTTGTTCGTGAAATCGCTCAGGATTTCAAAACAGATCTCCGATTCCAAAGCAGCGCTGTTTCTGCACTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAGGATACTAATCTCTGTGCCATTCATGCTAAGAGAGTTACTATCATGCCTAAGGATATTCAACTCGCTCGCAGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>MsG0580025504.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*