AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007919.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007919.01.T01 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.99e-96 272
MsG0280007919.01.T01 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.15e-95 275
MsG0280007919.01.T01 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0280007919.01.T01 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0280007919.01.T01 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0280007919.01.T01 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.44e-92 264
MsG0280007919.01.T01 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.38e-91 262
MsG0280007919.01.T01 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0280007919.01.T01 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.44e-86 248
MsG0280007919.01.T01 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.82e-82 238
MsG0280007919.01.T01 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.64e-75 219
MsG0280007919.01.T01 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.05e-65 195
MsG0280007919.01.T01 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.74e-56 171
MsG0280007919.01.T01 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.96e-52 160
MsG0280007919.01.T01 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.69e-45 144
MsG0280007919.01.T01 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.67e-43 139
MsG0280007919.01.T01 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.74e-42 137
MsG0280007919.01.T01 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.50e-40 132
MsG0280007919.01.T01 MTR_8g027840 55.102 98 43 1 37 133 28 125 5.01e-30 105
MsG0280007919.01.T01 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.48e-25 92.4
MsG0280007919.01.T01 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.75e-20 80.9
MsG0280007919.01.T01 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.26e-18 73.6
MsG0280007919.01.T01 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.81e-15 66.2
MsG0280007919.01.T01 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.48e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007919.01.T01 AT5G65360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0280007919.01.T01 AT5G10390 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0280007919.01.T01 AT5G10400 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0280007919.01.T01 AT3G27360 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0280007919.01.T01 AT1G09200 99.265 136 1 0 1 136 1 136 1.62e-95 271
MsG0280007919.01.T01 AT5G10980 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0280007919.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0280007919.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0280007919.01.T01 AT4G40040 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0280007919.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0280007919.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0280007919.01.T01 AT4G40030 97.059 136 4 0 1 136 1 136 3.59e-93 265
MsG0280007919.01.T01 AT4G40030 97.059 136 4 0 1 136 29 164 7.04e-93 266
MsG0280007919.01.T01 AT5G65350 94.853 136 7 0 1 136 1 136 5.30e-91 260
MsG0280007919.01.T01 AT1G75600 93.382 136 9 0 1 136 1 136 1.25e-89 256
MsG0280007919.01.T01 AT1G13370 93.382 136 9 0 1 136 1 136 5.55e-89 254
MsG0280007919.01.T01 AT1G19890 89.781 137 13 1 1 136 1 137 1.07e-83 241
MsG0280007919.01.T01 AT5G12910 71.111 135 34 2 1 135 1 130 9.89e-67 198
MsG0280007919.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0280007919.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0280007919.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110
MsG0280007919.01.T01 AT1G01370 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 41 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTGGCCGGAGCGGATTTC+CGG 0.220285 2:-22959070 None:intergenic
TCACGAAGAGCAACGGTTCC+TGG 0.297872 2:-22959122 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 2:+22959278 MsG0280007919.01.T01:CDS
GGTGGCCGGAGCGGATTTCC+GGG 0.357421 2:-22959069 None:intergenic
GAAGCTTCCGTTTCAAAGAT+TGG 0.362056 2:+22959184 MsG0280007919.01.T01:CDS
TTCTTCACTCCTCCGGTGGC+CGG 0.400280 2:-22959083 None:intergenic
GGATTTGCGGGCTGTTTGTT+TGG 0.425629 2:-22959003 None:intergenic
CGGAAATCCGCTCCGGCCAC+CGG 0.457345 2:+22959071 MsG0280007919.01.T01:CDS
CACTCCTCCGGTGGCCGGAG+CGG 0.467671 2:-22959078 None:intergenic
TTGTGGCGAGTTGTTTGCGT+GGG 0.485319 2:-22959040 None:intergenic
CTCGCAAGTTGAATATCCTT+AGG 0.495447 2:-22959356 None:intergenic
GTGGGGCTTCTTCACTCCTC+CGG 0.496815 2:-22959090 None:intergenic
GAGCAGAAACAGCACTACTC+TGG 0.498444 2:-22959247 None:intergenic
AAGCCCCACCGTTTCCGTCC+AGG 0.502650 2:+22959104 MsG0280007919.01.T01:CDS
TCTCGAACCAATCTTTGAAA+CGG 0.505611 2:-22959191 None:intergenic
TTGTGCTATTCATGCTAAGA+GGG 0.513784 2:+22959322 MsG0280007919.01.T01:CDS
CAACGGTTCCTGGACGGAAA+CGG 0.513929 2:-22959112 None:intergenic
AGCCGCCCGGAAATCCGCTC+CGG 0.514500 2:+22959064 MsG0280007919.01.T01:CDS
CAAACAGCCCGCAAATCCAC+CGG 0.524480 2:+22959008 MsG0280007919.01.T01:CDS
AATTGCTCAGGATTTCAAAA+CGG 0.529430 2:+22959214 MsG0280007919.01.T01:CDS
ATTGGTTCGAGAAATTGCTC+AGG 0.529480 2:+22959202 MsG0280007919.01.T01:CDS
AGAGCACTGAGCTTCTCATA+AGG 0.531497 2:+22959162 MsG0280007919.01.T01:CDS
AAACAGCACTACTCTGGAAA+CGG 0.532210 2:-22959241 None:intergenic
GAGGGTTACTATTATGCCTA+AGG 0.533362 2:+22959340 MsG0280007919.01.T01:CDS
CGGTTCCTGGACGGAAACGG+TGG 0.534819 2:-22959109 None:intergenic
TTTGTGCTATTCATGCTAAG+AGG 0.537531 2:+22959321 MsG0280007919.01.T01:CDS
GGTTCCTGGACGGAAACGGT+GGG 0.550288 2:-22959108 None:intergenic
GCGGATTTCACGAAGAGCAA+CGG 0.553291 2:-22959129 None:intergenic
CAGTGCTCTTCTGATACTTG+CGG 0.560252 2:-22959148 None:intergenic
GAGAAGAATCAGAGGCGAAA+GGG 0.568256 2:+22959376 MsG0280007919.01.T01:CDS
CGAGAAGAATCAGAGGCGAA+AGG 0.575054 2:+22959375 MsG0280007919.01.T01:CDS
GGGCTTCTTCACTCCTCCGG+TGG 0.600889 2:-22959087 None:intergenic
AACTCGCCACAAAAGCCGCC+CGG 0.602698 2:+22959051 MsG0280007919.01.T01:CDS
AAATCCGCTCCGGCCACCGG+AGG 0.609902 2:+22959074 MsG0280007919.01.T01:CDS
GTTCCTGGACGGAAACGGTG+GGG 0.616311 2:-22959107 None:intergenic
GAAGAGCAACGGTTCCTGGA+CGG 0.646875 2:-22959118 None:intergenic
TTTGTGGCGAGTTGTTTGCG+TGG 0.648955 2:-22959041 None:intergenic
ACAGCCCGCAAATCCACCGG+CGG 0.662314 2:+22959011 MsG0280007919.01.T01:CDS
CAACTTGCGAGAAGAATCAG+AGG 0.665987 2:+22959368 MsG0280007919.01.T01:CDS
GGCCGGAGCGGATTTCCGGG+CGG 0.692981 2:-22959066 None:intergenic
TGTGGCGAGTTGTTTGCGTG+GGG 0.709822 2:-22959039 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATTGCTCAGGATTTCAAAA+CGG + Chr2:22959214-22959233 MsG0280007919.01.T01:CDS 30.0%
! TCTCGAACCAATCTTTGAAA+CGG - Chr2:22959194-22959213 None:intergenic 35.0%
! TTGTGCTATTCATGCTAAGA+GGG + Chr2:22959322-22959341 MsG0280007919.01.T01:CDS 35.0%
! TTTGTGCTATTCATGCTAAG+AGG + Chr2:22959321-22959340 MsG0280007919.01.T01:CDS 35.0%
GAAGCTTCCGTTTCAAAGAT+TGG + Chr2:22959184-22959203 MsG0280007919.01.T01:CDS 40.0%
GAGGGTTACTATTATGCCTA+AGG + Chr2:22959340-22959359 MsG0280007919.01.T01:CDS 40.0%
! AAACAGCACTACTCTGGAAA+CGG - Chr2:22959244-22959263 None:intergenic 40.0%
! CTCGCAAGTTGAATATCCTT+AGG - Chr2:22959359-22959378 None:intergenic 40.0%
!! ATTGGTTCGAGAAATTGCTC+AGG + Chr2:22959202-22959221 MsG0280007919.01.T01:CDS 40.0%
CAACTTGCGAGAAGAATCAG+AGG + Chr2:22959368-22959387 MsG0280007919.01.T01:CDS 45.0%
CAGTGCTCTTCTGATACTTG+CGG - Chr2:22959151-22959170 None:intergenic 45.0%
GAGAAGAATCAGAGGCGAAA+GGG + Chr2:22959376-22959395 MsG0280007919.01.T01:CDS 45.0%
GCTGCTGAAGCTTATCTTGT+TGG + Chr2:22959278-22959297 MsG0280007919.01.T01:CDS 45.0%
!! AGAGCACTGAGCTTCTCATA+AGG + Chr2:22959162-22959181 MsG0280007919.01.T01:CDS 45.0%
CGAGAAGAATCAGAGGCGAA+AGG + Chr2:22959375-22959394 MsG0280007919.01.T01:CDS 50.0%
GAGCAGAAACAGCACTACTC+TGG - Chr2:22959250-22959269 None:intergenic 50.0%
GCGGATTTCACGAAGAGCAA+CGG - Chr2:22959132-22959151 None:intergenic 50.0%
TTGTGGCGAGTTGTTTGCGT+GGG - Chr2:22959043-22959062 None:intergenic 50.0%
TTTGTGGCGAGTTGTTTGCG+TGG - Chr2:22959044-22959063 None:intergenic 50.0%
! GGATTTGCGGGCTGTTTGTT+TGG - Chr2:22959006-22959025 None:intergenic 50.0%
CAAACAGCCCGCAAATCCAC+CGG + Chr2:22959008-22959027 MsG0280007919.01.T01:CDS 55.0%
CAACGGTTCCTGGACGGAAA+CGG - Chr2:22959115-22959134 None:intergenic 55.0%
GAAGAGCAACGGTTCCTGGA+CGG - Chr2:22959121-22959140 None:intergenic 55.0%
TCACGAAGAGCAACGGTTCC+TGG - Chr2:22959125-22959144 None:intergenic 55.0%
TGTGGCGAGTTGTTTGCGTG+GGG - Chr2:22959042-22959061 None:intergenic 55.0%
!! CTTTTCCGCCGGTGGATTTG+CGG - Chr2:22959019-22959038 None:intergenic 55.0%
!! TTTTCCGCCGGTGGATTTGC+GGG - Chr2:22959018-22959037 None:intergenic 55.0%
AACTCGCCACAAAAGCCGCC+CGG + Chr2:22959051-22959070 MsG0280007919.01.T01:CDS 60.0%
GGTTCCTGGACGGAAACGGT+GGG - Chr2:22959111-22959130 None:intergenic 60.0%
GTGGGGCTTCTTCACTCCTC+CGG - Chr2:22959093-22959112 None:intergenic 60.0%
GTTCCTGGACGGAAACGGTG+GGG - Chr2:22959110-22959129 None:intergenic 60.0%
TTCTTCACTCCTCCGGTGGC+CGG - Chr2:22959086-22959105 None:intergenic 60.0%
! GGATTTCCGGGCGGCTTTTG+TGG - Chr2:22959060-22959079 None:intergenic 60.0%
! TTTGCGTGGGGCTTTTCCGC+CGG - Chr2:22959030-22959049 None:intergenic 60.0%
AAGCCCCACCGTTTCCGTCC+AGG + Chr2:22959104-22959123 MsG0280007919.01.T01:CDS 65.0%
ACAGCCCGCAAATCCACCGG+CGG + Chr2:22959011-22959030 MsG0280007919.01.T01:CDS 65.0%
CGGTTCCTGGACGGAAACGG+TGG - Chr2:22959112-22959131 None:intergenic 65.0%
GGGCTTCTTCACTCCTCCGG+TGG - Chr2:22959090-22959109 None:intergenic 65.0%
AAATCCGCTCCGGCCACCGG+AGG + Chr2:22959074-22959093 MsG0280007919.01.T01:CDS 70.0%
AGCCGCCCGGAAATCCGCTC+CGG + Chr2:22959064-22959083 MsG0280007919.01.T01:CDS 70.0%
CGGAAATCCGCTCCGGCCAC+CGG + Chr2:22959071-22959090 MsG0280007919.01.T01:CDS 70.0%
! CGGTGGCCGGAGCGGATTTC+CGG - Chr2:22959073-22959092 None:intergenic 70.0%
! GGTGGCCGGAGCGGATTTCC+GGG - Chr2:22959072-22959091 None:intergenic 70.0%
CACTCCTCCGGTGGCCGGAG+CGG - Chr2:22959081-22959100 None:intergenic 75.0%
! GCGTGGGGCTTTTCCGCCGG+TGG - Chr2:22959027-22959046 None:intergenic 75.0%
! GGCCGGAGCGGATTTCCGGG+CGG - Chr2:22959069-22959088 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 22958993 22959403 22958993 ID=MsG0280007919.01;Name=MsG0280007919.01
Chr2 mRNA 22958993 22959403 22958993 ID=MsG0280007919.01.T01;Parent=MsG0280007919.01;Name=MsG0280007919.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|136
Chr2 exon 22958993 22959403 22958993 ID=MsG0280007919.01.T01:exon:13905;Parent=MsG0280007919.01.T01
Chr2 CDS 22958993 22959403 22958993 ID=MsG0280007919.01.T01:cds;Parent=MsG0280007919.01.T01
Gene Sequence

>MsG0280007919.01.T01

ATGGCACGTACCAAACAAACAGCCCGCAAATCCACCGGCGGAAAAGCCCCACGCAAACAACTCGCCACAAAAGCCGCCCGGAAATCCGCTCCGGCCACCGGAGGAGTGAAGAAGCCCCACCGTTTCCGTCCAGGAACCGTTGCTCTTCGTGAAATCCGCAAGTATCAGAAGAGCACTGAGCTTCTCATAAGGAAGCTTCCGTTTCAAAGATTGGTTCGAGAAATTGCTCAGGATTTCAAAACGGATCTCCGTTTCCAGAGTAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGTGCTATTCATGCTAAGAGGGTTACTATTATGCCTAAGGATATTCAACTTGCGAGAAGAATCAGAGGCGAAAGGGCTTAG

Protein sequence

>MsG0280007919.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*