AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018239.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018239.01.T01 MTR_4g008050 92.000 250 20 0 1 250 1 250 1.07e-157 438
MsG0480018239.01.T01 MTR_4g046640 50.000 66 33 0 8 73 36 101 2.00e-15 71.2
MsG0480018239.01.T01 MTR_2g089310 50.000 66 33 0 6 71 3 68 3.22e-12 64.7
MsG0480018239.01.T01 MTR_2g011450 48.276 58 30 0 6 63 3 60 5.34e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018239.01.T01 AT3G21150 32.051 234 129 3 8 241 5 208 1.90e-28 108
MsG0480018239.01.T01 AT4G27310 47.500 80 41 1 6 85 3 81 2.46e-15 73.2
MsG0480018239.01.T01 AT3G21890 51.613 62 30 0 7 68 30 91 5.70e-15 69.7
MsG0480018239.01.T01 AT5G54470 52.000 75 35 1 3 77 1 74 6.85e-15 71.6
MsG0480018239.01.T01 AT4G15248 44.156 77 43 0 8 84 32 108 1.30e-14 68.6

Find 65 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGGTTGGAGAAAATATC+AGG 0.275267 4:-2063937 MsG0480018239.01.T01:CDS
TTCGGTGAAACCGTCGCATT+TGG 0.282536 4:+2064422 None:intergenic
GGTGCCAGCTAAGCTGATTT+TGG 0.291167 4:-2063914 MsG0480018239.01.T01:CDS
GCGAAATGGAGAAGAGAGTT+AGG 0.346518 4:-2064120 MsG0480018239.01.T01:CDS
CCGATTCCGCCTTTCTCTGC+CGG 0.365012 4:-2064512 MsG0480018239.01.T01:CDS
AAGAAGATGATCGGGGATAA+AGG 0.373096 4:-2064171 MsG0480018239.01.T01:CDS
CACACCCTTCTTGCAATTCA+AGG 0.376949 4:+2063854 None:intergenic
TTCTTATTGTTCTTGTTCTC+CGG 0.377556 4:+2064192 None:intergenic
TGGCTGCTGCGACGTCGTTT+TGG 0.398824 4:-2064011 MsG0480018239.01.T01:CDS
TTGTTCTTGTTCTCCGGTGC+CGG 0.401998 4:+2064198 None:intergenic
ACAATAAGAAGAAGATGATC+GGG 0.406679 4:-2064179 MsG0480018239.01.T01:CDS
CGAAATGGAGAAGAGAGTTA+GGG 0.429114 4:-2064119 MsG0480018239.01.T01:CDS
GAGAACGTGGAAGTTACTTC+CGG 0.435197 4:-2064040 MsG0480018239.01.T01:CDS
AGCTGCGACGACGCCGTTCA+CGG 0.441391 4:-2064489 MsG0480018239.01.T01:CDS
CGGAGGAGATATTTGCGAAA+TGG 0.441862 4:-2064134 MsG0480018239.01.T01:CDS
CCGGCAGAGAAAGGCGGAAT+CGG 0.453844 4:+2064512 None:intergenic
TTCCGGTGAGCAAGAGCGGC+AGG 0.464761 4:+2064356 None:intergenic
AACAATAAGAAGAAGATGAT+CGG 0.468382 4:-2064180 MsG0480018239.01.T01:CDS
GAACGGCGTCGTCGCAGCTC+CGG 0.476153 4:+2064493 None:intergenic
GTCGCAGCAGCCACCTTCAC+CGG 0.482296 4:+2064021 None:intergenic
AGTGCCGGAGATATGAATTT+CGG 0.485587 4:+2064404 None:intergenic
GTCGCAGCTCCGGCAGAGAA+AGG 0.496029 4:+2064503 None:intergenic
GTGGCCAAAATCAGCTTAGC+TGG 0.499961 4:+2063910 None:intergenic
CGGGGATAAAGGTGTTGAAT+CGG 0.508934 4:-2064160 MsG0480018239.01.T01:CDS
TTCACCGAAATTCATATCTC+CGG 0.509514 4:-2064408 MsG0480018239.01.T01:CDS
AGGTGTTGAATCGGTGGCGG+AGG 0.520405 4:-2064151 MsG0480018239.01.T01:CDS
GTGTCGAAATTTGAAGAGGT+TGG 0.526675 4:-2063950 MsG0480018239.01.T01:CDS
GCCACACGATCACCGTTCAC+CGG 0.535889 4:+2064087 None:intergenic
AACCTGCCGCTCTTGCTCAC+CGG 0.536391 4:-2064358 MsG0480018239.01.T01:CDS
CCACGTGTCGAAATTTGAAG+AGG 0.538451 4:-2063954 MsG0480018239.01.T01:CDS
CGATGATACATCTCCGGCAC+CGG 0.539008 4:-2064211 MsG0480018239.01.T01:CDS
GAACGGTGATCGTGTGGCGG+TGG 0.541705 4:-2064082 MsG0480018239.01.T01:CDS
CAGAGAAAGGCGGAATCGGA+AGG 0.554491 4:+2064516 None:intergenic
ACGGTGGTGTAGTGTAGTGC+CGG 0.554858 4:+2064389 None:intergenic
AACACCTTGAATTGCAAGAA+GGG 0.556767 4:-2063858 MsG0480018239.01.T01:CDS
TCACCGGAAAATCATTCCTC+CGG 0.557681 4:-2064342 MsG0480018239.01.T01:CDS
TCGTGTGGCGGTGGAAGCTA+TGG 0.558085 4:-2064073 MsG0480018239.01.T01:CDS
AGATTGAGAACTCGCCTCAC+CGG 0.561454 4:+2064323 None:intergenic
AGAGGTTGGAGAAAATATCA+GGG 0.568817 4:-2063936 MsG0480018239.01.T01:CDS
TCCGGTGAACGGTGATCGTG+TGG 0.570131 4:-2064088 MsG0480018239.01.T01:CDS
CAACACCTTGAATTGCAAGA+AGG 0.572050 4:-2063859 MsG0480018239.01.T01:CDS
GTGGAAGTTACTTCCGGTGA+AGG 0.577957 4:-2064034 MsG0480018239.01.T01:CDS
GGATAAAGGTGTTGAATCGG+TGG 0.581180 4:-2064157 MsG0480018239.01.T01:CDS
TAAAGGTGTTGAATCGGTGG+CGG 0.581743 4:-2064154 MsG0480018239.01.T01:CDS
GAAGTTACTTCCGGTGAAGG+TGG 0.596707 4:-2064031 MsG0480018239.01.T01:CDS
GAGGAAGTTAGCGCCGTGAA+CGG 0.601829 4:+2064476 None:intergenic
ATCTTGTGCTCCAAATGCGA+CGG 0.602290 4:-2064432 MsG0480018239.01.T01:CDS
AGCGGCAGGTTGATGAGAGA+CGG 0.602586 4:+2064370 None:intergenic
CCTCTTCAAATTTCGACACG+TGG 0.606211 4:+2063954 None:intergenic
CGGAGATGTATCATCGGTAA+CGG 0.606238 4:+2064218 None:intergenic
CGTTACCGATGATACATCTC+CGG 0.613031 4:-2064217 MsG0480018239.01.T01:CDS
ATTTGGAGCACAAGATGTGA+CGG 0.631824 4:+2064439 None:intergenic
TGTGGCGGTGGAAGCTATGG+TGG 0.634915 4:-2064070 MsG0480018239.01.T01:CDS
GCAGCTCCGGCAGAGAAAGG+CGG 0.638220 4:+2064506 None:intergenic
TTGAGAACTCGCCTCACCGG+AGG 0.643923 4:+2064326 None:intergenic
TGGTGGTGTGTTTGAGAACG+TGG 0.644878 4:-2064053 MsG0480018239.01.T01:CDS
CGGTGCCGGAGATGTATCAT+CGG 0.645343 4:+2064212 None:intergenic
GGTGAACGGTGATCGTGTGG+CGG 0.678941 4:-2064085 MsG0480018239.01.T01:CDS
GGAAAATCATTCCTCCGGTG+AGG 0.685941 4:-2064337 MsG0480018239.01.T01:CDS
ACGTGCGAGTTTCACATGTG+TGG 0.686351 4:+2063891 None:intergenic
CAATAAGAAGAAGATGATCG+GGG 0.688051 4:-2064178 MsG0480018239.01.T01:CDS
GGCAGGTTGATGAGAGACGG+TGG 0.691500 4:+2064373 None:intergenic
GAGGTTGGAGAAAATATCAG+GGG 0.717043 4:-2063935 MsG0480018239.01.T01:CDS
TGGAGCACAAGATGTGACGG+AGG 0.724489 4:+2064442 None:intergenic
GACGGAGGTGACGAGCGACG+AGG 0.727759 4:+2064457 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACAATAAGAAGAAGATGAT+CGG - Chr4:2064236-2064255 MsG0480018239.01.T01:CDS 25.0%
ACAATAAGAAGAAGATGATC+GGG - Chr4:2064237-2064256 MsG0480018239.01.T01:CDS 30.0%
TTCTTATTGTTCTTGTTCTC+CGG + Chr4:2064227-2064246 None:intergenic 30.0%
AACACCTTGAATTGCAAGAA+GGG - Chr4:2064558-2064577 MsG0480018239.01.T01:CDS 35.0%
AAGAGGTTGGAGAAAATATC+AGG - Chr4:2064479-2064498 MsG0480018239.01.T01:CDS 35.0%
AGAGGTTGGAGAAAATATCA+GGG - Chr4:2064480-2064499 MsG0480018239.01.T01:CDS 35.0%
CAATAAGAAGAAGATGATCG+GGG - Chr4:2064238-2064257 MsG0480018239.01.T01:CDS 35.0%
TTCACCGAAATTCATATCTC+CGG - Chr4:2064008-2064027 MsG0480018239.01.T01:CDS 35.0%
! AGAGTTAGGGTTAGATTTTC+CGG - Chr4:2064310-2064329 MsG0480018239.01.T01:CDS 35.0%
AAGAAGATGATCGGGGATAA+AGG - Chr4:2064245-2064264 MsG0480018239.01.T01:CDS 40.0%
CAACACCTTGAATTGCAAGA+AGG - Chr4:2064557-2064576 MsG0480018239.01.T01:CDS 40.0%
CGAAATGGAGAAGAGAGTTA+GGG - Chr4:2064297-2064316 MsG0480018239.01.T01:CDS 40.0%
GAGGTTGGAGAAAATATCAG+GGG - Chr4:2064481-2064500 MsG0480018239.01.T01:CDS 40.0%
GTGTCGAAATTTGAAGAGGT+TGG - Chr4:2064466-2064485 MsG0480018239.01.T01:CDS 40.0%
! AGTGCCGGAGATATGAATTT+CGG + Chr4:2064015-2064034 None:intergenic 40.0%
! ATTTGGAGCACAAGATGTGA+CGG + Chr4:2063980-2063999 None:intergenic 40.0%
! GGTTGGGGTTAAGATTTTGT+GGG - Chr4:2064426-2064445 MsG0480018239.01.T01:CDS 40.0%
! GTTGGGGTTAAGATTTTGTG+GGG - Chr4:2064427-2064446 MsG0480018239.01.T01:CDS 40.0%
! TGGTTGGGGTTAAGATTTTG+TGG - Chr4:2064425-2064444 MsG0480018239.01.T01:CDS 40.0%
ATCTTGTGCTCCAAATGCGA+CGG - Chr4:2063984-2064003 MsG0480018239.01.T01:CDS 45.0%
CACACCCTTCTTGCAATTCA+AGG + Chr4:2064565-2064584 None:intergenic 45.0%
CCACGTGTCGAAATTTGAAG+AGG - Chr4:2064462-2064481 MsG0480018239.01.T01:CDS 45.0%
CCTCTTCAAATTTCGACACG+TGG + Chr4:2064465-2064484 None:intergenic 45.0%
CGGAGATGTATCATCGGTAA+CGG + Chr4:2064201-2064220 None:intergenic 45.0%
CGGAGGAGATATTTGCGAAA+TGG - Chr4:2064282-2064301 MsG0480018239.01.T01:CDS 45.0%
CGTTACCGATGATACATCTC+CGG - Chr4:2064199-2064218 MsG0480018239.01.T01:CDS 45.0%
GAGAACGTGGAAGTTACTTC+CGG - Chr4:2064376-2064395 MsG0480018239.01.T01:CDS 45.0%
GCGAAATGGAGAAGAGAGTT+AGG - Chr4:2064296-2064315 MsG0480018239.01.T01:CDS 45.0%
TCACCGGAAAATCATTCCTC+CGG - Chr4:2064074-2064093 MsG0480018239.01.T01:CDS 45.0%
! GGGTTAGATTTTCCGGTGAA+CGG - Chr4:2064317-2064336 MsG0480018239.01.T01:CDS 45.0%
! TCACCGGAGGAATGATTTTC+CGG + Chr4:2064080-2064099 None:intergenic 45.0%
!! CGGGGATAAAGGTGTTGAAT+CGG - Chr4:2064256-2064275 MsG0480018239.01.T01:CDS 45.0%
!! GGATAAAGGTGTTGAATCGG+TGG - Chr4:2064259-2064278 MsG0480018239.01.T01:CDS 45.0%
!! TAAAGGTGTTGAATCGGTGG+CGG - Chr4:2064262-2064281 MsG0480018239.01.T01:CDS 45.0%
ACGTGCGAGTTTCACATGTG+TGG + Chr4:2064528-2064547 None:intergenic 50.0%
AGATTGAGAACTCGCCTCAC+CGG + Chr4:2064096-2064115 None:intergenic 50.0%
GAAGTTACTTCCGGTGAAGG+TGG - Chr4:2064385-2064404 MsG0480018239.01.T01:CDS 50.0%
GGAAAATCATTCCTCCGGTG+AGG - Chr4:2064079-2064098 MsG0480018239.01.T01:CDS 50.0%
GTGGAAGTTACTTCCGGTGA+AGG - Chr4:2064382-2064401 MsG0480018239.01.T01:CDS 50.0%
GTGGCCAAAATCAGCTTAGC+TGG + Chr4:2064509-2064528 None:intergenic 50.0%
TTCGGTGAAACCGTCGCATT+TGG + Chr4:2063997-2064016 None:intergenic 50.0%
TTGTTCTTGTTCTCCGGTGC+CGG + Chr4:2064221-2064240 None:intergenic 50.0%
! GATTTTCCGGTGAGCAAGAG+CGG + Chr4:2064067-2064086 None:intergenic 50.0%
! TGGTGGTGTGTTTGAGAACG+TGG - Chr4:2064363-2064382 MsG0480018239.01.T01:CDS 50.0%
!! GGTGCCAGCTAAGCTGATTT+TGG - Chr4:2064502-2064521 MsG0480018239.01.T01:CDS 50.0%
ACGGTGGTGTAGTGTAGTGC+CGG + Chr4:2064030-2064049 None:intergenic 55.0%
CAGAGAAAGGCGGAATCGGA+AGG + Chr4:2063903-2063922 None:intergenic 55.0%
CGATGATACATCTCCGGCAC+CGG - Chr4:2064205-2064224 MsG0480018239.01.T01:CDS 55.0%
GAGGAAGTTAGCGCCGTGAA+CGG + Chr4:2063943-2063962 None:intergenic 55.0%
TGGAGCACAAGATGTGACGG+AGG + Chr4:2063977-2063996 None:intergenic 55.0%
! CGGTGCCGGAGATGTATCAT+CGG + Chr4:2064207-2064226 None:intergenic 55.0%
!! AGCGGCAGGTTGATGAGAGA+CGG + Chr4:2064049-2064068 None:intergenic 55.0%
!! TGCTGCGACGTCGTTTTGGT+TGG - Chr4:2064409-2064428 MsG0480018239.01.T01:CDS 55.0%
!!! CTGCGACGTCGTTTTGGTTG+GGG - Chr4:2064411-2064430 MsG0480018239.01.T01:CDS 55.0%
!!! GCTGCGACGTCGTTTTGGTT+GGG - Chr4:2064410-2064429 MsG0480018239.01.T01:CDS 55.0%
AACCTGCCGCTCTTGCTCAC+CGG - Chr4:2064058-2064077 MsG0480018239.01.T01:CDS 60.0%
CCGATTCCGCCTTTCTCTGC+CGG - Chr4:2063904-2063923 MsG0480018239.01.T01:CDS 60.0%
CCGGCAGAGAAAGGCGGAAT+CGG + Chr4:2063907-2063926 None:intergenic 60.0%
GCCACACGATCACCGTTCAC+CGG + Chr4:2064332-2064351 None:intergenic 60.0%
GGTGAACGGTGATCGTGTGG+CGG - Chr4:2064331-2064350 MsG0480018239.01.T01:CDS 60.0%
TCCGGTGAACGGTGATCGTG+TGG - Chr4:2064328-2064347 MsG0480018239.01.T01:CDS 60.0%
TCGTGTGGCGGTGGAAGCTA+TGG - Chr4:2064343-2064362 MsG0480018239.01.T01:CDS 60.0%
TGTGGCGGTGGAAGCTATGG+TGG - Chr4:2064346-2064365 MsG0480018239.01.T01:CDS 60.0%
TTGAGAACTCGCCTCACCGG+AGG + Chr4:2064093-2064112 None:intergenic 60.0%
! TGGCTGCTGCGACGTCGTTT+TGG - Chr4:2064405-2064424 MsG0480018239.01.T01:CDS 60.0%
!! AGGTGTTGAATCGGTGGCGG+AGG - Chr4:2064265-2064284 MsG0480018239.01.T01:CDS 60.0%
!! GGCAGGTTGATGAGAGACGG+TGG + Chr4:2064046-2064065 None:intergenic 60.0%
AGCTGCGACGACGCCGTTCA+CGG - Chr4:2063927-2063946 MsG0480018239.01.T01:CDS 65.0%
GAACGGTGATCGTGTGGCGG+TGG - Chr4:2064334-2064353 MsG0480018239.01.T01:CDS 65.0%
GCAGCTCCGGCAGAGAAAGG+CGG + Chr4:2063913-2063932 None:intergenic 65.0%
GTCGCAGCAGCCACCTTCAC+CGG + Chr4:2064398-2064417 None:intergenic 65.0%
GTCGCAGCTCCGGCAGAGAA+AGG + Chr4:2063916-2063935 None:intergenic 65.0%
TTCCGGTGAGCAAGAGCGGC+AGG + Chr4:2064063-2064082 None:intergenic 65.0%
GAACGGCGTCGTCGCAGCTC+CGG + Chr4:2063926-2063945 None:intergenic 70.0%
GACGGAGGTGACGAGCGACG+AGG + Chr4:2063962-2063981 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 2063843 2064595 2063843 ID=MsG0480018239.01;Name=MsG0480018239.01
Chr4 mRNA 2063843 2064595 2063843 ID=MsG0480018239.01.T01;Parent=MsG0480018239.01;Name=MsG0480018239.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|250
Chr4 exon 2063843 2064595 2063843 ID=MsG0480018239.01.T01:exon:681;Parent=MsG0480018239.01.T01
Chr4 CDS 2063843 2064595 2063843 ID=MsG0480018239.01.T01:cds;Parent=MsG0480018239.01.T01
Gene Sequence

>MsG0480018239.01.T01

ATGAAGATGAAGTGCAAACAATGCGAACTTTGTAATCAACAAGCTTCTTTCTATTGTCCTTCCGATTCCGCCTTTCTCTGCCGGAGCTGCGACGACGCCGTTCACGGCGCTAACTTCCTCGTCGCTCGTCACCTCCGTCACATCTTGTGCTCCAAATGCGACGGTTTCACCGAAATTCATATCTCCGGCACTACACTACACCACCGTCTCTCATCAACCTGCCGCTCTTGCTCACCGGAAAATCATTCCTCCGGTGAGGCGAGTTCTCAATCTTCTTCATCTTGCGAGTCTTGCGTTACAGAAAAGAAGAAAACAAAATCTCGAAAGATAATGAAGAGTTTTTCAGTTTCGAATTCCGTTACCGATGATACATCTCCGGCACCGGAGAACAAGAACAATAAGAAGAAGATGATCGGGGATAAAGGTGTTGAATCGGTGGCGGAGGAGATATTTGCGAAATGGAGAAGAGAGTTAGGGTTAGATTTTCCGGTGAACGGTGATCGTGTGGCGGTGGAAGCTATGGTGGTGTGTTTGAGAACGTGGAAGTTACTTCCGGTGAAGGTGGCTGCTGCGACGTCGTTTTGGTTGGGGTTAAGATTTTGTGGGGACAATAGTTTTGCCACGTGTCGAAATTTGAAGAGGTTGGAGAAAATATCAGGGGTGCCAGCTAAGCTGATTTTGGCCACACATGTGAAACTCGCACGTGTCTTTACTCAACACCTTGAATTGCAAGAAGGGTGTGATGAGTCCTAA

Protein sequence

>MsG0480018239.01.T01

MKMKCKQCELCNQQASFYCPSDSAFLCRSCDDAVHGANFLVARHLRHILCSKCDGFTEIHISGTTLHHRLSSTCRSCSPENHSSGEASSQSSSSCESCVTEKKKTKSRKIMKSFSVSNSVTDDTSPAPENKNNKKKMIGDKGVESVAEEIFAKWRRELGLDFPVNGDRVAVEAMVVCLRTWKLLPVKVAAATSFWLGLRFCGDNSFATCRNLKRLEKISGVPAKLILATHVKLARVFTQHLELQEGCDES*