AlfalfaGEDB | MsG0880043763.01

M. sativa cultivar ZhongmuNo.1 / MsG0880043763.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043763.01.T01	AFK39185.1	57	100	5	1	15	114	38	99	1.78E-26	105

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043763.01.T01	G7LHM1	59.000	100	3	1	15	114	146	207	1.66e-26	108
MsG0880043763.01.T01	I3SFZ3	57.000	100	5	1	15	114	38	99	8.48e-27	105

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003624.01	MsG0880043763.01	0.881203	2.917623e-70	1.693719e-66
MsG0780041812.01	MsG0880043763.01	0.864938	8.478684e-65	2.709418e-61
MsG0880042893.01	MsG0880043763.01	0.815923	7.209682e-52	5.036950e-49
MsG0280010620.01	MsG0880043763.01	0.802624	5.136150e-49	2.527872e-46
MsG0280010642.01	MsG0880043763.01	0.894738	1.858638e-75	1.871654e-71
MsG0280011113.01	MsG0880043763.01	0.833540	5.029789e-56	5.810267e-53
MsG0380013116.01	MsG0880043763.01	0.909271	6.848098e-82	1.322029e-77
MsG0680034837.01	MsG0880043763.01	0.858878	6.088970e-63	1.577360e-59
MsG0480018239.01	MsG0880043763.01	0.813950	1.974222e-51	1.307246e-48
MsG0580026191.01	MsG0880043763.01	0.824634	7.258265e-54	6.466325e-51
MsG0780036754.01	MsG0880043763.01	0.839223	1.802256e-57	2.472555e-54
MsG0780036755.01	MsG0880043763.01	0.831600	1.522465e-55	1.660259e-52
MsG0780036756.01	MsG0880043763.01	0.837740	4.348343e-57	5.701947e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043763.01.T01	MTR_8g040640	59.000	100	3	1	15	114	146	207	4.21e-30	108
MsG0880043763.01.T01	MTR_8g040640	59.000	100	3	1	15	114	233	294	6.54e-29	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 22 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAATGCTACCTTTGTTTAT+AGG	0.217765	8:+30274261	MsG0880043763.01.T01:CDS
TGCATGAACAATCTCTTAGT+TGG	0.367739	8:-30274379	None:intergenic
GTTGCTAATCTCATGTTTAA+CGG	0.416032	8:+30274335	MsG0880043763.01.T01:CDS
TCGATTGACAGTGAGATTGT+CGG	0.471668	8:+30274010	MsG0880043763.01.T01:CDS
CTGTTGGTCTCTGATTCCAT+TGG	0.476213	8:-30274299	None:intergenic
GCATGCTAACATCACCATCC+AGG	0.540288	8:-30273908	None:intergenic
CATGCAAGAGGCAATGCCAT+GGG	0.575721	8:+30273926	MsG0880043763.01.T01:CDS
GCTTCATTGTGAACTGCCCA+TGG	0.576976	8:-30273942	None:intergenic
GCATGCAAGAGGCAATGCCA+TGG	0.580050	8:+30273925	MsG0880043763.01.T01:CDS
AACGGCTCTGTCGACACTGT+TGG	0.582134	8:-30274315	None:intergenic
TCAGAATGTAGTAGAGGACC+TGG	0.590986	8:+30273890	MsG0880043763.01.T01:CDS
CAGTGAGATTGTCGGAATCA+CGG	0.591728	8:+30274018	MsG0880043763.01.T01:CDS
AAATCTAAATCTATACCTCA+AGG	0.596472	8:-30273964	None:intergenic
GTTAAACATGAGATTAGCAA+CGG	0.610661	8:-30274333	None:intergenic
GTCTGACGACAAGAAACCAA+TGG	0.611234	8:+30274283	MsG0880043763.01.T01:CDS
AAAACATCAGAATGTAGTAG+AGG	0.618804	8:+30273884	MsG0880043763.01.T01:CDS
TTGGATCAGCATGCAATATG+CGG	0.639912	8:-30274360	None:intergenic
GTCGTCAGACCTATAAACAA+AGG	0.646735	8:-30274270	None:intergenic
GGTCAGGCAGTGCAATTCTG+AGG	0.656482	8:+30274408	MsG0880043763.01.T01:CDS
AATGTAGTAGAGGACCTGGA+TGG	0.664719	8:+30273894	MsG0880043763.01.T01:CDS
CAGTTCACAATGAAGCCTTG+AGG	0.672028	8:+30273949	MsG0880043763.01.T01:CDS
TGGTGATGTTAGCATGCAAG+AGG	0.691812	8:+30273914	MsG0880043763.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATCTAAATCTATACCTCA+AGG	-	Chr8:30273967-30273986	None:intergenic	25.0%
!	TTTACAAAATCAAGATCATC+AGG	-	Chr8:30274183-30274202	None:intergenic	25.0%
!!	AAAGAAAATAGAAGTGTGTT+TGG	-	Chr8:30274112-30274131	None:intergenic	25.0%
!!	TCAAACACATACTTTAAGTT+TGG	-	Chr8:30274137-30274156	None:intergenic	25.0%
	AAAACATCAGAATGTAGTAG+AGG	+	Chr8:30273884-30273903	MsG0880043763.01.T01:CDS	30.0%
	GAGATTGTTCATGCAAAAAT+TGG	+	Chr8:30274387-30274406	MsG0880043763.01.T01:CDS	30.0%
	GTTAAACATGAGATTAGCAA+CGG	-	Chr8:30274336-30274355	None:intergenic	30.0%
	GTTGCTAATCTCATGTTTAA+CGG	+	Chr8:30274335-30274354	MsG0880043763.01.T01:CDS	30.0%
!	ATTCTGATGTTTTGTATCTC+TGG	-	Chr8:30273877-30273896	None:intergenic	30.0%
!	CAAATGCTACCTTTGTTTAT+AGG	+	Chr8:30274261-30274280	MsG0880043763.01.T01:CDS	30.0%
	TGCATGAACAATCTCTTAGT+TGG	-	Chr8:30274382-30274401	None:intergenic	35.0%
	TGTTCATGCAAAAATTGGTC+AGG	+	Chr8:30274392-30274411	MsG0880043763.01.T01:CDS	35.0%
!	TAGAAGTGTGTTTGGTTGAA+CGG	-	Chr8:30274104-30274123	None:intergenic	35.0%
	GTCGTCAGACCTATAAACAA+AGG	-	Chr8:30274273-30274292	None:intergenic	40.0%
	TCGATTGACAGTGAGATTGT+CGG	+	Chr8:30274010-30274029	MsG0880043763.01.T01:CDS	40.0%
	TTGGATCAGCATGCAATATG+CGG	-	Chr8:30274363-30274382	None:intergenic	40.0%
!	TGATGTTTTGTATCTCTGGC+AGG	-	Chr8:30273873-30273892	None:intergenic	40.0%
	AATGTAGTAGAGGACCTGGA+TGG	+	Chr8:30273894-30273913	MsG0880043763.01.T01:CDS	45.0%
	CAGTGAGATTGTCGGAATCA+CGG	+	Chr8:30274018-30274037	MsG0880043763.01.T01:CDS	45.0%
	CAGTTCACAATGAAGCCTTG+AGG	+	Chr8:30273949-30273968	MsG0880043763.01.T01:CDS	45.0%
	CGGTGAGTTTGGCAAAATCA+CGG	-	Chr8:30274084-30274103	None:intergenic	45.0%
	GTCTGACGACAAGAAACCAA+TGG	+	Chr8:30274283-30274302	MsG0880043763.01.T01:CDS	45.0%
	GTTTGGTTGAACGGTGAGTT+TGG	-	Chr8:30274095-30274114	None:intergenic	45.0%
	TCAGAATGTAGTAGAGGACC+TGG	+	Chr8:30273890-30273909	MsG0880043763.01.T01:CDS	45.0%
!	CTGTTGGTCTCTGATTCCAT+TGG	-	Chr8:30274302-30274321	None:intergenic	45.0%
!	TGGTGATGTTAGCATGCAAG+AGG	+	Chr8:30273914-30273933	MsG0880043763.01.T01:CDS	45.0%
!	TGTTTTGTATCTCTGGCAGG+CGG	-	Chr8:30273870-30273889	None:intergenic	45.0%
	CATGCAAGAGGCAATGCCAT+GGG	+	Chr8:30273926-30273945	MsG0880043763.01.T01:CDS	50.0%
	GCATGCTAACATCACCATCC+AGG	-	Chr8:30273911-30273930	None:intergenic	50.0%
	GCTTCATTGTGAACTGCCCA+TGG	-	Chr8:30273945-30273964	None:intergenic	50.0%
	GGAATCACGGTCAGACACTT+TGG	+	Chr8:30274031-30274050	MsG0880043763.01.T01:intron	50.0%
	AACGGCTCTGTCGACACTGT+TGG	-	Chr8:30274318-30274337	None:intergenic	55.0%
	GCATGCAAGAGGCAATGCCA+TGG	+	Chr8:30273925-30273944	MsG0880043763.01.T01:CDS	55.0%
	GGTCAGGCAGTGCAATTCTG+AGG	+	Chr8:30274408-30274427	MsG0880043763.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	30273831	30274433	30273831	ID=MsG0880043763.01;Name=MsG0880043763.01
Chr8	mRNA	30273831	30274433	30273831	ID=MsG0880043763.01.T01;Parent=MsG0880043763.01;Name=MsG0880043763.01.T01;_AED=0.50;_eAED=0.74;_QI=0\|0\|0\|1\|0\|0.5\|2\|0\|134
Chr8	exon	30273831	30274039	30273831	ID=MsG0880043763.01.T01:exon:9175;Parent=MsG0880043763.01.T01
Chr8	exon	30274238	30274433	30274238	ID=MsG0880043763.01.T01:exon:9176;Parent=MsG0880043763.01.T01
Chr8	CDS	30273831	30274039	30273831	ID=MsG0880043763.01.T01:cds;Parent=MsG0880043763.01.T01
Chr8	CDS	30274238	30274433	30274238	ID=MsG0880043763.01.T01:cds;Parent=MsG0880043763.01.T01

Gene Sequence

>MsG0880043763.01.T01

ATGACAAGACTCGAATTCACTTCGCGATGCACTTTACCGCCTGCCAGAGATACAAAACATCAGAATGTAGTAGAGGACCTGGATGGTGATGTTAGCATGCAAGAGGCAATGCCATGGGCAGTTCACAATGAAGCCTTGAGGTATAGATTTAGATTTGCTGCAAACTTTTCGTGCATTTTTCGATTGACAGTGAGATTGTCGGAATCACGTAAACGAAGACACACACTTAACTCAAATGCTACCTTTGTTTATAGGTCTGACGACAAGAAACCAATGGAATCAGAGACCAACAGTGTCGACAGAGCCGTTGCTAATCTCATGTTTAACGGAACCGCATATTGCATGCTGATCCAACTAAGAGATTGTTCATGCAAAAATTGGTCAGGCAGTGCAATTCTGAGGTGA

Protein sequence

>MsG0880043763.01.T01

MTRLEFTSRCTLPPARDTKHQNVVEDLDGDVSMQEAMPWAVHNEALRYRFRFAANFSCIFRLTVRLSESRKRRHTLNSNATFVYRSDDKKPMESETNSVDRAVANLMFNGTAYCMLIQLRDCSCKNWSGSAILR*