AlfalfaGEDB | MsG0480018860.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018860.01

KEGG: -
GO: -
PFAM: PF05056

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018860.01.T01	XP_024638070.2	90.076	524	35	4	1	521	1	510	0	920

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018860.01.T01	A0A396I2M9	90.076	524	35	4	1	521	1	510	0.0	920

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
MsG0480018548.01	MsG0480018860.01	PPI
MsG0480018550.01	MsG0480018860.01	PPI
MsG0480018551.01	MsG0480018860.01	PPI
MsG0480018553.01	MsG0480018860.01	PPI
MsG0480018554.01	MsG0480018860.01	PPI
MsG0480018558.01	MsG0480018860.01	PPI
MsG0480018719.01	MsG0480018860.01	PPI
MsG0480018724.01	MsG0480018860.01	PPI
MsG0480018735.01	MsG0480018860.01	PPI
MsG0480018736.01	MsG0480018860.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018860.01.T01	MTR_4g019340	86.600	500	31	4	11	507	37	503	0.0	835
MsG0480018860.01.T01	MTR_4g017830	73.410	519	118	5	1	517	1	501	0.0	738
MsG0480018860.01.T01	MTR_4g017440	65.631	515	150	7	4	513	6	498	0.0	636
MsG0480018860.01.T01	MTR_4g017410	64.583	528	140	11	1	521	1	488	0.0	622
MsG0480018860.01.T01	MTR_4g017470	60.152	527	151	6	1	521	1	474	0.0	574
MsG0480018860.01.T01	MTR_4g017550	64.050	484	141	11	39	517	18	473	0.0	559
MsG0480018860.01.T01	MTR_4g017550	28.846	260	137	10	6	247	242	471	4.82e-12	68.6
MsG0480018860.01.T01	MTR_4g017610	59.339	514	166	11	2	513	3	475	0.0	554
MsG0480018860.01.T01	MTR_4g019310	42.453	530	244	17	1	513	1	486	1.91e-117	356
MsG0480018860.01.T01	MTR_7g066260	34.026	529	261	15	5	518	8	463	4.28e-77	251

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018860.01.T01	AT3G09140	31.676	543	274	17	9	513	6	489	6.38e-59	203
MsG0480018860.01.T01	AT3G09140	27.917	240	145	10	270	491	5	234	1.02e-11	67.8
MsG0480018860.01.T01	AT5G01120	30.902	521	303	14	9	513	6	485	4.17e-58	201
MsG0480018860.01.T01	AT5G43240	31.955	532	279	18	13	513	10	489	1.04e-55	195
MsG0480018860.01.T01	AT5G43240	31.955	532	279	18	13	513	10	489	1.04e-55	195
MsG0480018860.01.T01	AT5G43240	31.955	532	279	18	13	513	10	489	1.04e-55	195
MsG0480018860.01.T01	AT5G01130	31.287	505	276	14	2	503	4	440	1.13e-55	193
MsG0480018860.01.T01	AT3G09140	30.123	571	274	18	9	513	6	517	1.34e-53	190
MsG0480018860.01.T01	AT3G09140	27.055	292	164	12	270	525	5	283	8.54e-13	71.2
MsG0480018860.01.T01	AT5G01150	30.189	530	294	17	2	513	4	475	4.18e-49	177

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Find 108 sgRNAs with CRISPR-Local

Find 295 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACATTTGTTTGCAGAC+AGG	0.485916	4:+10708254	None:intergenic
AACGGTAGAATTAAAGATAG+AGG	0.436841	4:-10705153	MsG0480018860.01.T01:CDS
AATGATTCACATGGATTCTT+CGG	0.347163	4:+10708227	None:intergenic
AATTTGGATTCTGAGGGTCA+GGG	0.610725	4:-10708068	MsG0480018860.01.T01:CDS
ACAACATTTGTTTGCAGACA+GGG	0.497506	4:+10708255	None:intergenic
ACAGGGTTCCAAACAACGTT+TGG	0.415978	4:+10708272	None:intergenic
ACCATCCAAAACCTTTACAA+TGG	0.583435	4:+10705403	None:intergenic
ACCATTAGGGTCCATTGTAA+AGG	0.460196	4:-10705414	MsG0480018860.01.T01:CDS
ACCTCACTCCAAACGTTGTT+TGG	0.290443	4:-10708280	MsG0480018860.01.T01:CDS
ACCTTTACAATGGACCCTAA+TGG	0.477568	4:+10705413	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATACATGTTATGATTGGG+TGG	-	Chr4:10708230-10708249	MsG0480018860.01.T01:CDS	30.0%
AACAGCAAGTTATGAAGTTA+AGG	+	Chr4:10705861-10705880	None:intergenic	30.0%
AACGGTAGAATTAAAGATAG+AGG	-	Chr4:10707716-10707735	MsG0480018860.01.T01:intron	30.0%
AATACATGTTATGATTGGGT+GGG	-	Chr4:10708231-10708250	MsG0480018860.01.T01:CDS	30.0%
AATATAGTAGGAGGGAATAA+GGG	-	Chr4:10706030-10706049	MsG0480018860.01.T01:intron	30.0%
AATGATTCACATGGATTCTT+CGG	+	Chr4:10704645-10704664	None:intergenic	30.0%
ACAGAGAAACAGAATATAGT+AGG	-	Chr4:10706018-10706037	MsG0480018860.01.T01:intron	30.0%
ACGGTAGAATTAAAGATAGA+GGG	-	Chr4:10707717-10707736	MsG0480018860.01.T01:intron	30.0%
AGAAACAGAATATAGTAGGA+GGG	-	Chr4:10706022-10706041	MsG0480018860.01.T01:intron	30.0%
AGAGAAAGATTTATCCTTCA+AGG	-	Chr4:10706295-10706314	MsG0480018860.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	10704336	10708555	10704336	ID=MsG0480018860.01;Name=MsG0480018860.01
Chr4	mRNA	10704336	10708555	10704336	ID=MsG0480018860.01.T01;Parent=MsG0480018860.01;Name=MsG0480018860.01.T01;_AED=0.33;_eAED=0.35;_QI=0\|0\|0\|1\|0\|0.33\|3\|0\|528
Chr4	exon	10707878	10708555	10707878	ID=MsG0480018860.01.T01:exon:3610;Parent=MsG0480018860.01.T01
Chr4	exon	10704905	10705678	10704905	ID=MsG0480018860.01.T01:exon:3609;Parent=MsG0480018860.01.T01
Chr4	exon	10704336	10704470	10704336	ID=MsG0480018860.01.T01:exon:3608;Parent=MsG0480018860.01.T01
Chr4	CDS	10707878	10708555	10707878	ID=MsG0480018860.01.T01:cds;Parent=MsG0480018860.01.T01
Chr4	CDS	10704905	10705678	10704905	ID=MsG0480018860.01.T01:cds;Parent=MsG0480018860.01.T01
Chr4	CDS	10704336	10704470	10704336	ID=MsG0480018860.01.T01:cds;Parent=MsG0480018860.01.T01

Gene Sequence

>MsG0480018860.01.T01

ATGGCTTCTGAAGAACCACAAGAAGAGTCAAAAACGATTCCACTGACAATCTTAATAGACAGAGAAAACAACAAAGTTGTCGCTGTTGAATCAACCAAAGACTTCGTTGACACTCTCTTCAGCTTCCTTTCTCTTCCACTTGCTACTATCATTCGCCTCTTAACCACCCAGAACAATGATCAACAACAACAAGAATCCTCAGAATCATCATCATTCCTCGGAAGCATCAAAAACCTCTATGAAAGCGTACAAAACCTCACTCCAAACGTTGTTTGGAACCCTGTCTGCAAACAAATGTTGTTGAATCCGAAGAATCCATGTGAATCATTATGCAAGAAGCTTTTCTTGAACATTGATGACACTGAATGGTCAAGTAAGTTTTTCGTGTGTGATGCTTGTAACAATTTTACAACTTTTCAGAATCTTGATTGTACTTGTGGAAAACCCACTAATAAACAGCCTAAGAATTTGGATTCTGAGGGTCAGGGGAATAGTGGTGGTATTGCTGATGCTTTAAATGGGGTTTTTGTTAAGGACAATGGGTTTTTGTTTTTGGTGTATGATGATTTGAAGATTGTGCCAAGTTCTTTGGTGACTTCCATGCAGATGTTGATGGAATTGGGGTATTCTGATTTGAGTCAATTAGAGGAAGTCACACGCAACATTGGAAAACAGGAGATGCTGAACCTCCTCAAGTACACCTTAACCTCTCATGAGCCTCTGACAAATACAATTTTGAAAAGCAGCTCCAAGAACAAAGACAACCCGCCAAACCAGTTTGCATCAGCAGTCAAAGTAAAAACTTGCACCCGCGACATTAAAATGGACGTTAAGCTAGTGCAAAGTAAATCTCAGAAAAAGATAATCTGTGCAGAAGCCTGTGGAAACTTTGTTGACTTCATGTTTAGCTTCCTTACAATACCATTAGGGTCCATTGTAAAGGTTTTGGATGGTAACTCTTTTGTTGGATGTGTTGATAATTTGTACAAGAGTGTAGAGAGCTTGGACTCTTCGTGGTGTACAAACTCGCGCTCCATGTTACTCAATCCTGGTGTCGCCCCACACTTTGATTGTCCAAACCAGCCCCTCAACATTCCTCATGTACATCCACCACTCACTACATATTATTATGGTACAGGAACATCAAAGCAAGAGTATAATTATAGTTATCGTTGTTATCAAACGGTAGAATTAAAGATAGAGGGAGGAGTGATTTCTAAGTCGCATGGATTGATTTATAAAGCAAACGCCTTGACTGAAATGGACCCAAGATCCCCCAACAAATTAAAAAAAGATGTGGTGGGATTTGTGAAGAGACCGGCATTATATAGTGTAGGAGATGATCTAAAAGTAAAACCACTTTCTGCTAATTCTTGCCTTTCATATTTGAAAGAATTAAACCTTCCCCTTGTTGATCTTGAAGTGAAGGTGATAAGCATTGGTGAAGCTGAGGATTCTTACCTCTTGATTTGCAATTTGCAGGCTTTGAGCCTCCTTGCAGCTTCCTTGACATCAAAATTTACACTGACTAGTGGACTGGAAGAATTTTTTAACGAGCAAAAAGACCTCAAAGTGTGTTCAAACTAG

Protein sequence

>MsG0480018860.01.T01

MASEEPQEESKTIPLTILIDRENNKVVAVESTKDFVDTLFSFLSLPLATIIRLLTTQNNDQQQQESSESSSFLGSIKNLYESVQNLTPNVVWNPVCKQMLLNPKNPCESLCKKLFLNIDDTEWSSKFFVCDACNNFTTFQNLDCTCGKPTNKQPKNLDSEGQGNSGGIADALNGVFVKDNGFLFLVYDDLKIVPSSLVTSMQMLMELGYSDLSQLEEVTRNIGKQEMLNLLKYTLTSHEPLTNTILKSSSKNKDNPPNQFASAVKVKTCTRDIKMDVKLVQSKSQKKIICAEACGNFVDFMFSFLTIPLGSIVKVLDGNSFVGCVDNLYKSVESLDSSWCTNSRSMLLNPGVAPHFDCPNQPLNIPHVHPPLTTYYYGTGTSKQEYNYSYRCYQTVELKIEGGVISKSHGLIYKANALTEMDPRSPNKLKKDVVGFVKRPALYSVGDDLKVKPLSANSCLSYLKELNLPLVDLEVKVISIGEAEDSYLLICNLQALSLLAASLTSKFTLTSGLEEFFNEQKDLKVCSN*