AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018558.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018558.01.T01 MTR_4g017410 70.476 315 78 2 1 304 1 311 4.40e-153 438
MsG0480018558.01.T01 MTR_4g017610 63.666 311 102 3 1 304 1 307 2.33e-133 387
MsG0480018558.01.T01 MTR_4g017440 66.234 308 97 2 4 304 5 312 6.93e-130 379
MsG0480018558.01.T01 MTR_4g017830 62.541 307 103 4 3 302 2 303 3.38e-127 373
MsG0480018558.01.T01 MTR_4g019340 62.046 303 93 5 12 297 37 334 1.96e-117 347
MsG0480018558.01.T01 MTR_4g019340 26.872 227 121 6 16 228 306 501 9.16e-12 65.9
MsG0480018558.01.T01 MTR_4g017550 61.702 282 91 7 27 297 3 278 4.96e-107 320
MsG0480018558.01.T01 MTR_4g017470 61.386 303 83 7 1 302 1 270 4.75e-106 318
MsG0480018558.01.T01 MTR_4g019310 37.812 320 153 11 1 297 1 297 6.57e-55 186
MsG0480018558.01.T01 MTR_4g019310 25.984 254 157 8 4 243 257 493 6.08e-12 66.2
MsG0480018558.01.T01 MTR_7g066350 37.624 303 164 8 7 297 3 292 7.35e-49 169
MsG0480018558.01.T01 MTR_7g066260 36.129 310 163 10 6 297 8 300 3.01e-48 168
MsG0480018558.01.T01 MTR_7g066260 27.391 230 123 8 12 236 268 458 3.29e-12 67.0
MsG0480018558.01.T01 MTR_7g066340 33.974 312 184 9 3 304 2 301 6.15e-47 164
MsG0480018558.01.T01 MTR_7g066290 36.808 307 163 8 6 297 8 298 2.27e-46 162
MsG0480018558.01.T01 MTR_7g058710 34.505 313 160 10 1 297 1 284 2.30e-46 163
MsG0480018558.01.T01 MTR_4g019240 61.789 123 43 2 1 123 1 119 4.18e-44 149
MsG0480018558.01.T01 MTR_7g012680 36.452 310 145 13 2 297 3 274 7.84e-44 155
MsG0480018558.01.T01 MTR_7g066570 37.097 310 160 14 1 297 1 288 4.82e-43 155
MsG0480018558.01.T01 MTR_1g041315 34.810 316 163 11 9 297 10 309 9.27e-41 148
MsG0480018558.01.T01 MTR_7g063660 35.099 302 162 13 14 304 10 288 5.57e-40 145
MsG0480018558.01.T01 MTR_7g066360 36.449 321 171 12 1 304 1 305 1.25e-38 142
MsG0480018558.01.T01 MTR_7g066430 34.219 301 164 10 14 297 5 288 1.33e-38 142
MsG0480018558.01.T01 MTR_7g066430 25.806 248 149 6 7 236 251 481 8.32e-12 65.9
MsG0480018558.01.T01 MTR_7g066580 33.101 287 143 11 19 297 1 246 4.60e-37 137
MsG0480018558.01.T01 MTR_7g066480 32.990 291 175 8 14 297 17 294 1.72e-34 130
MsG0480018558.01.T01 MTR_7g063880 31.847 314 174 9 1 304 1 284 6.18e-34 129
MsG0480018558.01.T01 MTR_7g063830 33.007 306 175 9 1 297 1 285 1.30e-33 128
MsG0480018558.01.T01 MTR_7g063900 32.203 295 171 9 12 297 9 283 1.62e-33 128
MsG0480018558.01.T01 MTR_7g066410 31.677 322 165 13 14 297 11 315 2.55e-33 128
MsG0480018558.01.T01 MTR_7g066400 33.007 306 156 12 9 302 8 276 3.58e-33 127
MsG0480018558.01.T01 MTR_7g066400 27.753 227 134 4 10 236 237 433 1.31e-12 68.2
MsG0480018558.01.T01 MTR_7g063840 31.081 296 175 9 10 297 7 281 4.34e-32 124
MsG0480018558.01.T01 MTR_7g063890 34.130 293 163 8 14 297 28 299 1.63e-31 123
MsG0480018558.01.T01 MTR_7g062300 31.046 306 177 10 2 297 6 287 8.87e-31 120
MsG0480018558.01.T01 MTR_2g029400 33.624 229 100 4 9 237 10 186 5.25e-30 117
MsG0480018558.01.T01 MTR_7g062280 30.915 317 173 13 3 297 5 297 8.04e-29 115
MsG0480018558.01.T01 MTR_7g063820 33.016 315 163 13 1 297 1 285 1.34e-28 114
MsG0480018558.01.T01 MTR_7g066490 31.973 294 160 10 14 297 17 280 1.48e-27 111
MsG0480018558.01.T01 MTR_1g014060 33.475 236 128 9 22 243 9 229 1.41e-26 108
MsG0480018558.01.T01 MTR_7g066310 30.375 293 119 11 14 297 8 224 2.88e-25 104
MsG0480018558.01.T01 MTR_7g058700 29.568 301 136 13 25 297 531 783 2.90e-25 106
MsG0480018558.01.T01 MTR_7g063920 29.180 305 165 8 1 304 1 255 1.28e-23 100
MsG0480018558.01.T01 MTR_7g063920 27.391 230 139 7 14 236 218 426 6.49e-12 66.2
MsG0480018558.01.T01 MTR_3g007740 29.392 296 167 7 14 297 10 275 3.32e-23 99.4
MsG0480018558.01.T01 MTR_7g063670 29.900 301 173 9 8 297 10 283 2.52e-22 97.1
MsG0480018558.01.T01 MTR_3g065200 26.644 289 158 8 14 297 4 243 1.98e-20 91.3
MsG0480018558.01.T01 MTR_7g062270 35.714 182 96 8 10 183 7 175 8.56e-18 83.2
MsG0480018558.01.T01 MTR_8g445860 27.843 255 146 6 1 237 1 235 2.80e-17 79.7
MsG0480018558.01.T01 MTR_1g115440 27.083 240 144 6 14 237 12 236 6.85e-17 78.6
MsG0480018558.01.T01 MTR_1g115455 27.273 242 137 8 14 237 10 230 5.89e-15 73.2
MsG0480018558.01.T01 MTR_6g006800 70.833 48 12 1 1 48 1 46 1.46e-14 67.8
MsG0480018558.01.T01 MTR_0150s0010 28.049 246 134 7 14 237 11 235 2.21e-14 71.6
MsG0480018558.01.T01 MTR_7g100420 47.619 63 33 0 13 75 39 101 1.17e-12 68.2
MsG0480018558.01.T01 MTR_1g115450 33.613 119 64 2 11 129 5 108 2.90e-12 63.5
MsG0480018558.01.T01 MTR_7g066280 29.268 205 112 7 102 297 1 181 3.09e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018558.01.T01 AT3G09110 33.667 300 172 9 10 298 6 289 1.20e-42 150
MsG0480018558.01.T01 AT5G01130 33.770 305 165 11 6 297 3 283 1.18e-41 150
MsG0480018558.01.T01 AT3G09120 31.908 304 175 8 10 297 3 290 2.21e-37 135
MsG0480018558.01.T01 AT5G43240 31.250 304 175 9 14 297 10 299 5.45e-35 132
MsG0480018558.01.T01 AT5G43240 31.250 304 175 9 14 297 10 299 5.45e-35 132
MsG0480018558.01.T01 AT5G43240 31.250 304 175 9 14 297 10 299 5.45e-35 132
MsG0480018558.01.T01 AT5G01150 31.068 309 180 7 7 298 3 295 4.26e-34 130
MsG0480018558.01.T01 AT5G01120 31.068 309 178 10 10 298 6 299 1.04e-31 124
MsG0480018558.01.T01 AT3G09140 29.938 324 179 12 7 298 3 310 6.45e-30 118
MsG0480018558.01.T01 AT3G09140 27.557 352 179 13 7 298 3 338 4.69e-25 105
MsG0480018558.01.T01 AT5G01140 28.383 303 155 11 16 298 12 272 8.90e-25 103
MsG0480018558.01.T01 AT5G37320 28.571 301 176 12 20 297 18 302 1.41e-22 97.8

Find 65 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGTCCACTAACACTTTC+AGG 0.174169 4:+6551631 None:intergenic
TGGCATACAGCCATGTAATT+TGG 0.190293 4:-6551217 MsG0480018558.01.T01:CDS
AGGTGGGTTGTCTTTGTTCT+TGG 0.216712 4:+6550776 None:intergenic
CACCAATAACAGAAGTAAAA+TGG 0.276087 4:-6550718 MsG0480018558.01.T01:CDS
TTGATCATTGTTGCTATTGA+TGG 0.298828 4:+6551506 None:intergenic
AGTAAGTCACATGGATTTCT+TGG 0.302049 4:+6551360 None:intergenic
TAGTGTGGCTGAAACGTTTC+TGG 0.310990 4:-6551169 MsG0480018558.01.T01:CDS
TTGCAGCTGTTGAAGGAATT+CGG 0.312659 4:-6551102 MsG0480018558.01.T01:CDS
TGCTATTGATGGTGGATAAA+AGG 0.331043 4:+6551517 None:intergenic
AGCTTTCTTACCATGCCATT+CGG 0.346241 4:-6550616 MsG0480018558.01.T01:CDS
ACCTCAATTCAGACGATGTT+TGG 0.367095 4:-6551416 MsG0480018558.01.T01:CDS
ATTCTGAGGGTCAGTTGAAT+AGG 0.371830 4:-6551194 MsG0480018558.01.T01:CDS
GCCTCTCACAAATACAATCT+TGG 0.374861 4:-6550808 MsG0480018558.01.T01:CDS
TGCAGCTGTTGAAGGAATTC+GGG 0.386713 4:-6551101 MsG0480018558.01.T01:CDS
CAGAAGTAAAATGGATGTTA+AGG 0.394664 4:-6550709 MsG0480018558.01.T01:CDS
GCTTTCTTACCATGCCATTC+GGG 0.404087 4:-6550615 MsG0480018558.01.T01:CDS
GAGAGGCTCGTTAGTGGTTA+AGG 0.413109 4:+6550824 None:intergenic
CATCGTCTGAATTGAGGTCT+TGG 0.415737 4:+6551421 None:intergenic
AAGGCATTACTCCAACTGTT+GGG 0.416829 4:+6550742 None:intergenic
GACATACTTGCAGCTGTTGA+AGG 0.421440 4:-6551109 MsG0480018558.01.T01:CDS
AAATTACATGGCTGTATGCC+AGG 0.426919 4:+6551219 None:intergenic
ACTGTTGGGGCAGATTGCTT+AGG 0.430716 4:+6550756 None:intergenic
GTTGGGGCAGATTGCTTAGG+TGG 0.447488 4:+6550759 None:intergenic
CCTCTCACAAATACAATCTT+GGG 0.454373 4:-6550807 MsG0480018558.01.T01:CDS
GATTCCCCTGAAAGTGTTAG+TGG 0.461151 4:-6551637 MsG0480018558.01.T01:CDS
TTTGTCCACTAACACTTTCA+GGG 0.476352 4:+6551632 None:intergenic
CAAGGCATTACTCCAACTGT+TGG 0.480733 4:+6550741 None:intergenic
TGGTTAAGGTATACTTGAGT+AGG 0.482160 4:+6550838 None:intergenic
TGGTGCCAAGTGGAAGAGAT+AGG 0.487529 4:+6551547 None:intergenic
GTTTCGAAAAGTAGTAAACT+TGG 0.491017 4:+6551266 None:intergenic
AGCTTCCTATCTCTTCCACT+TGG 0.491188 4:-6551552 MsG0480018558.01.T01:CDS
CGAAACCTTAACTGCACTTG+TGG 0.496385 4:-6551249 MsG0480018558.01.T01:CDS
TTCAGACTTGACTCAAATAG+AGG 0.500328 4:-6551076 MsG0480018558.01.T01:CDS
CCCTCAGAATCCAAATTACA+TGG 0.509122 4:+6551207 None:intergenic
TTGGGGCAGATTGCTTAGGT+GGG 0.511735 4:+6550760 None:intergenic
GTCTGAATTGAGGTCTTGGA+TGG 0.519900 4:+6551425 None:intergenic
GCCATGTAATTTGGATTCTG+AGG 0.519948 4:-6551208 MsG0480018558.01.T01:CDS
ATTTGTGAGAGGCTCGTTAG+TGG 0.522997 4:+6550818 None:intergenic
AAGCAATCTGCCCCAACAGT+TGG 0.525058 4:-6550753 MsG0480018558.01.T01:CDS
TTACTTCTGTTATTGGTGCA+AGG 0.527701 4:+6550723 None:intergenic
ATCATTGTTGCTATTGATGG+TGG 0.539459 4:+6551509 None:intergenic
ACCAAACATCGTCTGAATTG+AGG 0.548115 4:+6551415 None:intergenic
ATGGTGATGATTCTGAGAGT+TGG 0.552671 4:+6551478 None:intergenic
CCCAAGATTGTATTTGTGAG+AGG 0.572271 4:+6550807 None:intergenic
GAAGTCAACAAAATCTCCGT+TGG 0.575489 4:+6550644 None:intergenic
AATTACATGGCTGTATGCCA+GGG 0.577967 4:+6551220 None:intergenic
CCATGTAATTTGGATTCTGA+GGG 0.585557 4:-6551207 MsG0480018558.01.T01:CDS
TTGTCCACTAACACTTTCAG+GGG 0.591923 4:+6551633 None:intergenic
GAGGAAATCACACACAACAT+TGG 0.597623 4:-6551057 MsG0480018558.01.T01:CDS
AGGTCCCAAAAGCTTTACAA+TGG 0.609484 4:+6550591 None:intergenic
TACAATGGACCCGAATGGCA+TGG 0.610188 4:+6550606 None:intergenic
ATGATCTTTGTAGAAGCCAA+CGG 0.617543 4:-6550660 MsG0480018558.01.T01:CDS
AGGCATTACTCCAACTGTTG+GGG 0.618850 4:+6550743 None:intergenic
AAGTGTTAGTGGACAAACAA+AGG 0.621379 4:-6551626 MsG0480018558.01.T01:CDS
GGTCTTGGATGGTTTCGTAG+AGG 0.631330 4:+6551436 None:intergenic
ACACAACATTGGCAAGCAAG+AGG 0.636713 4:-6551046 MsG0480018558.01.T01:intron
TTGTTCGTGTTGAGAAGCCA+TGG 0.638508 4:+6551677 None:intergenic
AGCTTTACAATGGACCCGAA+TGG 0.646881 4:+6550601 None:intergenic
TCAGTTGAATAGGACTAGTG+TGG 0.666324 4:-6551184 MsG0480018558.01.T01:CDS
GGTGGATAAAAGGCGGACAA+TGG 0.668124 4:+6551527 None:intergenic
TTCATGCACAGTAAGTCACA+TGG 0.670403 4:+6551351 None:intergenic
ATTACATGGCTGTATGCCAG+GGG 0.676398 4:+6551221 None:intergenic
AGGCGGACAATGGTGCCAAG+TGG 0.686421 4:+6551537 None:intergenic
TATTGATGGTGGATAAAAGG+CGG 0.701028 4:+6551520 None:intergenic
TTACATGGCTGTATGCCAGG+GGG 0.730012 4:+6551222 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTAAGAATTGAAATTGAT+TGG + Chr4:6551353-6551372 None:intergenic 20.0%
! TCAATATCTATACATCAGAA+AGG + Chr4:6551421-6551440 None:intergenic 25.0%
!! ATCCATTTTACTTCTGTTAT+TGG + Chr4:6551567-6551586 None:intergenic 25.0%
!!! TTTTTGATGTTTTCAAGGAA+TGG + Chr4:6550824-6550843 None:intergenic 25.0%
AGAATTGAAATTGATTGGAC+AGG + Chr4:6551348-6551367 None:intergenic 30.0%
CACCAATAACAGAAGTAAAA+TGG - Chr4:6551562-6551581 MsG0480018558.01.T01:CDS 30.0%
CAGAAGTAAAATGGATGTTA+AGG - Chr4:6551571-6551590 MsG0480018558.01.T01:CDS 30.0%
GTAAAAAAATGTGACGACAA+AGG + Chr4:6551278-6551297 None:intergenic 30.0%
GTTTCGAAAAGTAGTAAACT+TGG + Chr4:6551017-6551036 None:intergenic 30.0%
TTGGACAGGTTAAAACAATA+AGG + Chr4:6551334-6551353 None:intergenic 30.0%
! AGAGGTTTTTGATGTTTTCA+AGG + Chr4:6550829-6550848 None:intergenic 30.0%
! TTGATCATTGTTGCTATTGA+TGG + Chr4:6550777-6550796 None:intergenic 30.0%
!!! AAGGAACAAAGTTGTTTTTG+TGG - Chr4:6550673-6550692 MsG0480018558.01.T01:CDS 30.0%
AGTAAGTCACATGGATTTCT+TGG + Chr4:6550923-6550942 None:intergenic 35.0%
ATGATCTTTGTAGAAGCCAA+CGG - Chr4:6551620-6551639 MsG0480018558.01.T01:CDS 35.0%
CCATGTAATTTGGATTCTGA+GGG - Chr4:6551073-6551092 MsG0480018558.01.T01:CDS 35.0%
CCTCTCACAAATACAATCTT+GGG - Chr4:6551473-6551492 MsG0480018558.01.T01:CDS 35.0%
GTGAAGCTTCATGATTAAGA+CGG + Chr4:6551312-6551331 None:intergenic 35.0%
TGAAGCTTCATGATTAAGAC+GGG + Chr4:6551311-6551330 None:intergenic 35.0%
TGGTTAAGGTATACTTGAGT+AGG + Chr4:6551445-6551464 None:intergenic 35.0%
TTACTTCTGTTATTGGTGCA+AGG + Chr4:6551560-6551579 None:intergenic 35.0%
TTCAGACTTGACTCAAATAG+AGG - Chr4:6551204-6551223 MsG0480018558.01.T01:CDS 35.0%
TTTGTCCACTAACACTTTCA+GGG + Chr4:6550651-6550670 None:intergenic 35.0%
! AAGTGTTAGTGGACAAACAA+AGG - Chr4:6550654-6550673 MsG0480018558.01.T01:CDS 35.0%
! ATCATTGTTGCTATTGATGG+TGG + Chr4:6550774-6550793 None:intergenic 35.0%
!! TATTGATGGTGGATAAAAGG+CGG + Chr4:6550763-6550782 None:intergenic 35.0%
!! TGCTATTGATGGTGGATAAA+AGG + Chr4:6550766-6550785 None:intergenic 35.0%
!! TGTTTTTGTGGAAGCAACAA+AGG - Chr4:6550685-6550704 MsG0480018558.01.T01:CDS 35.0%
AAAATGTGACGACAAAGGAG+AGG + Chr4:6551273-6551292 None:intergenic 40.0%
AAATGTGACGACAAAGGAGA+GGG + Chr4:6551272-6551291 None:intergenic 40.0%
AAATTACATGGCTGTATGCC+AGG + Chr4:6551064-6551083 None:intergenic 40.0%
AATTACATGGCTGTATGCCA+GGG + Chr4:6551063-6551082 None:intergenic 40.0%
ACCAAACATCGTCTGAATTG+AGG + Chr4:6550868-6550887 None:intergenic 40.0%
ACCTCAATTCAGACGATGTT+TGG - Chr4:6550864-6550883 MsG0480018558.01.T01:intron 40.0%
AGCTTTCTTACCATGCCATT+CGG - Chr4:6551664-6551683 MsG0480018558.01.T01:CDS 40.0%
CCCAAGATTGTATTTGTGAG+AGG + Chr4:6551476-6551495 None:intergenic 40.0%
CCCTCAGAATCCAAATTACA+TGG + Chr4:6551076-6551095 None:intergenic 40.0%
GAAGTCAACAAAATCTCCGT+TGG + Chr4:6551639-6551658 None:intergenic 40.0%
GAGGAAATCACACACAACAT+TGG - Chr4:6551223-6551242 MsG0480018558.01.T01:CDS 40.0%
GCCATGTAATTTGGATTCTG+AGG - Chr4:6551072-6551091 MsG0480018558.01.T01:CDS 40.0%
GCCTCTCACAAATACAATCT+TGG - Chr4:6551472-6551491 MsG0480018558.01.T01:CDS 40.0%
GTTTGTCCACTAACACTTTC+AGG + Chr4:6550652-6550671 None:intergenic 40.0%
TGGCATACAGCCATGTAATT+TGG - Chr4:6551063-6551082 MsG0480018558.01.T01:CDS 40.0%
TTCATGCACAGTAAGTCACA+TGG + Chr4:6550932-6550951 None:intergenic 40.0%
TTGCAGCTGTTGAAGGAATT+CGG - Chr4:6551178-6551197 MsG0480018558.01.T01:CDS 40.0%
TTGTCCACTAACACTTTCAG+GGG + Chr4:6550650-6550669 None:intergenic 40.0%
! AAGGCATTACTCCAACTGTT+GGG + Chr4:6551541-6551560 None:intergenic 40.0%
! ATGGTGATGATTCTGAGAGT+TGG + Chr4:6550805-6550824 None:intergenic 40.0%
! ATTCTGAGGGTCAGTTGAAT+AGG - Chr4:6551086-6551105 MsG0480018558.01.T01:CDS 40.0%
! GTTTTCCACAAGTGCAGTTA+AGG + Chr4:6551039-6551058 None:intergenic 40.0%
! TCAGTTGAATAGGACTAGTG+TGG - Chr4:6551096-6551115 MsG0480018558.01.T01:CDS 40.0%
ACACAACATTGGCAAGCAAG+AGG - Chr4:6551234-6551253 MsG0480018558.01.T01:CDS 45.0%
AGCTTCCTATCTCTTCCACT+TGG - Chr4:6550728-6550747 MsG0480018558.01.T01:CDS 45.0%
AGGCATTACTCCAACTGTTG+GGG + Chr4:6551540-6551559 None:intergenic 45.0%
ATTACATGGCTGTATGCCAG+GGG + Chr4:6551062-6551081 None:intergenic 45.0%
ATTTGTGAGAGGCTCGTTAG+TGG + Chr4:6551465-6551484 None:intergenic 45.0%
CATCGTCTGAATTGAGGTCT+TGG + Chr4:6550862-6550881 None:intergenic 45.0%
CGAAACCTTAACTGCACTTG+TGG - Chr4:6551031-6551050 MsG0480018558.01.T01:intron 45.0%
GACATACTTGCAGCTGTTGA+AGG - Chr4:6551171-6551190 MsG0480018558.01.T01:CDS 45.0%
GCTTTCTTACCATGCCATTC+GGG - Chr4:6551665-6551684 MsG0480018558.01.T01:CDS 45.0%
GTCTGAATTGAGGTCTTGGA+TGG + Chr4:6550858-6550877 None:intergenic 45.0%
TAGTGTGGCTGAAACGTTTC+TGG - Chr4:6551111-6551130 MsG0480018558.01.T01:CDS 45.0%
TGCAGCTGTTGAAGGAATTC+GGG - Chr4:6551179-6551198 MsG0480018558.01.T01:CDS 45.0%
! CAAGGCATTACTCCAACTGT+TGG + Chr4:6551542-6551561 None:intergenic 45.0%
!! AGGTGGGTTGTCTTTGTTCT+TGG + Chr4:6551507-6551526 None:intergenic 45.0%
!! GATTCCCCTGAAAGTGTTAG+TGG - Chr4:6550643-6550662 MsG0480018558.01.T01:CDS 45.0%
AAGCAATCTGCCCCAACAGT+TGG - Chr4:6551527-6551546 MsG0480018558.01.T01:CDS 50.0%
ACTGTTGGGGCAGATTGCTT+AGG + Chr4:6551527-6551546 None:intergenic 50.0%
GAGAGGCTCGTTAGTGGTTA+AGG + Chr4:6551459-6551478 None:intergenic 50.0%
GGTCTTGGATGGTTTCGTAG+AGG + Chr4:6550847-6550866 None:intergenic 50.0%
GGTGGATAAAAGGCGGACAA+TGG + Chr4:6550756-6550775 None:intergenic 50.0%
TACAATGGACCCGAATGGCA+TGG + Chr4:6551677-6551696 None:intergenic 50.0%
TTACATGGCTGTATGCCAGG+GGG + Chr4:6551061-6551080 None:intergenic 50.0%
TTGGGGCAGATTGCTTAGGT+GGG + Chr4:6551523-6551542 None:intergenic 50.0%
! TGGTGCCAAGTGGAAGAGAT+AGG + Chr4:6550736-6550755 None:intergenic 50.0%
GTTGGGGCAGATTGCTTAGG+TGG + Chr4:6551524-6551543 None:intergenic 55.0%
TGCACTTGTGGAAAACCCCC+TGG - Chr4:6551043-6551062 MsG0480018558.01.T01:intron 55.0%
! AGGCGGACAATGGTGCCAAG+TGG + Chr4:6550746-6550765 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 6550605 6551697 6550605 ID=MsG0480018558.01;Name=MsG0480018558.01
Chr4 mRNA 6550605 6551697 6550605 ID=MsG0480018558.01.T01;Parent=MsG0480018558.01;Name=MsG0480018558.01.T01;_AED=0.11;_eAED=0.11;_QI=0|0|0|1|1|1|2|0|304
Chr4 exon 6551047 6551697 6551047 ID=MsG0480018558.01.T01:exon:3937;Parent=MsG0480018558.01.T01
Chr4 exon 6550605 6550868 6550605 ID=MsG0480018558.01.T01:exon:3936;Parent=MsG0480018558.01.T01
Chr4 CDS 6551047 6551697 6551047 ID=MsG0480018558.01.T01:cds;Parent=MsG0480018558.01.T01
Chr4 CDS 6550605 6550868 6550605 ID=MsG0480018558.01.T01:cds;Parent=MsG0480018558.01.T01
Gene Sequence

>MsG0480018558.01.T01

ATGGCTTCTCAACACGAACAACATGAAGAGTCAAAAACGATTCCCCTGAAAGTGTTAGTGGACAAACAAAGGAACAAAGTTGTTTTTGTGGAAGCAACAAAGGACTTTGTTGACACTCTCTTCAGCTTCCTATCTCTTCCACTTGGCACCATTGTCCGCCTTTTATCCACCATCAATAGCAACAATGATCAACAAAACCAACTCTCAGAATCATCACCATTCCTTGAAAACATCAAAAACCTCTACGAAACCATCCAAGACCTCAATTCAGACGATGTTTGGTACAACTCTTTCTGCAAACATATGTTGTTGCATCCAAGAAATCCATGTGACTTACTGTGCATGAAGTTGTTCTTGAATATTGATGATACTGATACATCAAATAAATTTTTTGTGTGTCATGCTTGTTCCAAGTTTACTACTTTTCGAAACCTTAACTGCACTTGTGGAAAACCCCCTGGCATACAGCCATGTAATTTGGATTCTGAGGGTCAGTTGAATAGGACTAGTGTGGCTGAAACGTTTCTGGTGTGTGATGATTTGAAGATTGTGCATTGTTCGTATGAGACATACTTGCAGCTGTTGAAGGAATTCGGGTATTCAGACTTGACTCAAATAGAGGAAATCACACACAACATTGGCAAGCAAGAGATATTGAACCTACTCAAGTATACCTTAACCACTAACGAGCCTCTCACAAATACAATCTTGGGATGTAGCTCCAAGAACAAAGACAACCCACCTAAGCAATCTGCCCCAACAGTTGGAGTAATGCCTTGCACCAATAACAGAAGTAAAATGGATGTTAAGGTAGTGCAAAGTAAATCTCAGAAAAAGATGATCTTTGTAGAAGCCAACGGAGATTTTGTTGACTTCATTTTAGCTTTCTTACCATGCCATTCGGGTCCATTGTAA
Protein sequence

>MsG0480018558.01.T01

MASQHEQHEESKTIPLKVLVDKQRNKVVFVEATKDFVDTLFSFLSLPLGTIVRLLSTINSNNDQQNQLSESSPFLENIKNLYETIQDLNSDDVWYNSFCKHMLLHPRNPCDLLCMKLFLNIDDTDTSNKFFVCHACSKFTTFRNLNCTCGKPPGIQPCNLDSEGQLNRTSVAETFLVCDDLKIVHCSYETYLQLLKEFGYSDLTQIEEITHNIGKQEILNLLKYTLTTNEPLTNTILGCSSKNKDNPPKQSAPTVGVMPCTNNRSKMDVKVVQSKSQKKMIFVEANGDFVDFILAFLPCHSGPL*