AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018550.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018550.01.T01 MTR_4g017610 66.304 460 150 3 2 457 3 461 0.0 587
MsG0480018550.01.T01 MTR_4g017410 65.116 473 119 9 10 453 7 462 0.0 573
MsG0480018550.01.T01 MTR_4g017440 65.319 470 136 6 10 453 12 480 0.0 570
MsG0480018550.01.T01 MTR_4g017830 61.284 483 153 10 1 453 1 479 0.0 551
MsG0480018550.01.T01 MTR_4g017550 66.743 439 117 10 39 453 18 451 0.0 536
MsG0480018550.01.T01 MTR_4g019340 60.924 476 136 12 9 453 35 491 0.0 519
MsG0480018550.01.T01 MTR_4g017470 57.202 486 137 10 1 453 1 448 7.07e-168 482
MsG0480018550.01.T01 MTR_4g019310 39.787 470 243 13 13 453 9 467 4.17e-96 298
MsG0480018550.01.T01 MTR_7g066350 33.836 464 236 12 13 452 10 426 3.00e-68 225
MsG0480018550.01.T01 MTR_7g066570 35.256 468 244 17 13 449 15 454 3.30e-67 225
MsG0480018550.01.T01 MTR_7g066340 34.267 464 235 16 13 452 13 430 2.18e-64 215
MsG0480018550.01.T01 MTR_7g066340 27.236 246 135 8 10 252 261 465 5.21e-12 68.2
MsG0480018550.01.T01 MTR_7g058710 30.785 484 249 14 13 452 13 454 3.01e-60 204
MsG0480018550.01.T01 MTR_7g066260 33.261 463 241 18 13 449 16 436 3.27e-59 202
MsG0480018550.01.T01 MTR_7g066260 29.665 209 118 6 267 453 18 219 1.94e-12 69.3
MsG0480018550.01.T01 MTR_7g012680 33.839 461 225 17 13 449 15 419 1.14e-58 199
MsG0480018550.01.T01 MTR_1g041315 33.191 467 248 15 13 452 15 444 1.28e-58 200
MsG0480018550.01.T01 MTR_7g066360 33.772 456 236 14 13 449 15 423 9.86e-55 190
MsG0480018550.01.T01 MTR_7g066410 31.213 503 275 15 13 458 11 499 1.06e-54 191
MsG0480018550.01.T01 MTR_7g063900 32.347 473 261 18 11 449 9 456 7.23e-54 187
MsG0480018550.01.T01 MTR_7g063660 31.522 460 268 15 13 449 10 445 1.27e-52 184
MsG0480018550.01.T01 MTR_7g066430 30.608 477 283 13 9 452 1 462 1.50e-51 182
MsG0480018550.01.T01 MTR_7g066580 31.799 478 243 16 18 458 1 432 1.84e-51 180
MsG0480018550.01.T01 MTR_7g063890 31.210 471 264 17 13 449 28 472 3.58e-51 181
MsG0480018550.01.T01 MTR_7g066290 31.567 453 233 12 13 449 16 407 9.78e-49 172
MsG0480018550.01.T01 MTR_7g062280 30.689 479 260 16 13 449 11 459 2.16e-48 173
MsG0480018550.01.T01 MTR_7g066400 30.736 462 232 17 13 449 13 411 8.90e-48 170
MsG0480018550.01.T01 MTR_7g063880 30.568 458 264 15 13 449 12 436 9.15e-48 171
MsG0480018550.01.T01 MTR_7g066480 31.042 451 233 13 13 453 17 399 1.56e-46 167
MsG0480018550.01.T01 MTR_7g063840 31.092 476 256 19 5 449 7 441 4.26e-46 166
MsG0480018550.01.T01 MTR_7g058700 28.976 459 209 12 22 452 529 898 1.73e-44 167
MsG0480018550.01.T01 MTR_2g029400 29.892 465 208 13 13 449 15 389 2.05e-44 160
MsG0480018550.01.T01 MTR_7g062300 29.713 488 269 20 1 452 1 450 2.76e-44 161
MsG0480018550.01.T01 MTR_7g066310 31.670 461 186 16 13 452 8 360 4.62e-43 157
MsG0480018550.01.T01 MTR_7g063820 31.992 472 256 18 13 449 11 452 2.24e-42 157
MsG0480018550.01.T01 MTR_3g065200 29.638 469 247 16 13 460 4 410 4.66e-42 155
MsG0480018550.01.T01 MTR_7g063830 31.757 444 246 16 13 432 11 421 4.86e-42 155
MsG0480018550.01.T01 MTR_4g019240 61.789 123 44 2 1 122 1 121 5.61e-42 147
MsG0480018550.01.T01 MTR_3g007740 29.828 466 253 17 9 452 6 419 9.68e-38 143
MsG0480018550.01.T01 MTR_7g063920 28.125 448 256 12 13 449 12 404 3.14e-37 141
MsG0480018550.01.T01 MTR_7g066490 28.825 451 237 11 13 457 17 389 5.79e-37 140
MsG0480018550.01.T01 MTR_7g063670 30.917 469 249 20 3 449 10 425 7.01e-37 141
MsG0480018550.01.T01 MTR_1g014060 28.825 451 198 18 21 449 9 358 8.11e-33 128
MsG0480018550.01.T01 MTR_7g066280 27.020 396 218 12 99 452 1 367 8.18e-25 106
MsG0480018550.01.T01 MTR_7g066680 31.538 260 141 8 220 453 37 285 5.34e-22 97.1
MsG0480018550.01.T01 MTR_7g062270 34.673 199 100 9 13 200 11 190 3.93e-19 89.4
MsG0480018550.01.T01 MTR_8g445860 28.689 244 142 7 10 240 11 235 3.76e-16 78.2
MsG0480018550.01.T01 MTR_8g445860 28.713 202 113 6 270 449 20 212 2.64e-15 75.9
MsG0480018550.01.T01 MTR_7g063720 31.414 191 95 6 273 449 32 200 4.01e-16 78.2
MsG0480018550.01.T01 MTR_1g115440 27.197 239 149 7 13 240 12 236 1.58e-14 73.6
MsG0480018550.01.T01 MTR_4g019330 29.885 174 72 3 278 450 32 156 3.86e-14 71.2
MsG0480018550.01.T01 MTR_8g023240 23.209 349 218 11 12 340 37 355 1.40e-13 73.2
MsG0480018550.01.T01 MTR_6g006800 74.468 47 11 1 1 47 1 46 4.52e-13 64.7
MsG0480018550.01.T01 MTR_0150s0010 28.333 240 145 7 13 240 11 235 1.70e-12 67.4
MsG0480018550.01.T01 MTR_1g115450 43.836 73 32 2 270 334 14 85 8.05e-12 63.2
MsG0480018550.01.T01 MTR_1g115435 27.602 221 120 8 10 225 5 190 1.17e-11 64.7
MsG0480018550.01.T01 MTR_1g115455 35.252 139 71 5 13 145 10 135 1.82e-11 64.3
MsG0480018550.01.T01 MTR_7g100420 28.627 255 140 11 220 449 134 371 3.69e-11 65.1
MsG0480018550.01.T01 MTR_7g100420 67.442 43 14 0 12 54 39 81 4.07e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018550.01.T01 AT5G01120 33.684 475 264 15 13 453 10 467 6.31e-65 218
MsG0480018550.01.T01 AT5G43240 34.025 482 257 19 13 453 10 471 1.40e-57 198
MsG0480018550.01.T01 AT5G43240 34.025 482 257 19 13 453 10 471 1.40e-57 198
MsG0480018550.01.T01 AT5G43240 34.025 482 257 19 13 453 10 471 1.40e-57 198
MsG0480018550.01.T01 AT5G01130 33.111 450 253 16 13 452 10 421 4.55e-54 187
MsG0480018550.01.T01 AT3G09140 30.769 481 280 16 10 453 7 471 5.10e-52 183
MsG0480018550.01.T01 AT5G01150 30.962 478 259 15 15 453 12 457 1.58e-50 179
MsG0480018550.01.T01 AT3G09110 35.583 326 176 11 15 325 12 318 3.99e-48 169
MsG0480018550.01.T01 AT3G09140 29.077 509 280 17 10 453 7 499 7.84e-47 170
MsG0480018550.01.T01 AT5G01140 30.937 459 248 15 15 449 12 425 2.25e-46 167
MsG0480018550.01.T01 AT3G09120 33.945 327 181 11 10 318 4 313 5.70e-43 154
MsG0480018550.01.T01 AT5G37320 28.416 461 283 16 19 453 18 457 9.06e-36 138
MsG0480018550.01.T01 AT5G43240 31.098 328 183 11 160 453 9 327 1.08e-28 116

Find 90 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTATTACAAATACAATTT+TGG 0.077846 4:+6495812 MsG0480018550.01.T01:CDS
CATCGTCTGAGTTGAGATTT+TGG 0.163224 4:-6494823 None:intergenic
CCCAAAATTGTATTTGTAAT+AGG 0.221679 4:-6495813 None:intergenic
AAATTCTTAGGTTGTCTATT+AGG 0.255481 4:-6495025 None:intergenic
TAATAGACAACCTAAGAATT+TGG 0.261898 4:+6495027 MsG0480018550.01.T01:CDS
GTGGGTTGTGGTATTAAATA+AGG 0.297283 4:-6496161 None:intergenic
CTCTAACTACTGATGCAAAT+TGG 0.299041 4:-6495869 None:intergenic
AGCTTCCTTACCATGCCATT+GGG 0.310690 4:+6495990 MsG0480018550.01.T01:CDS
TGTTGTCAGTGGTGGCTAAA+AGG 0.330255 4:-6494733 None:intergenic
ATCTCAACTCAGACGATGTT+TGG 0.331962 4:+6494828 MsG0480018550.01.T01:CDS
CTTAGAGATGTTGGTTCCAT+TGG 0.338874 4:+6495159 MsG0480018550.01.T01:CDS
CCACAACCCACGTATTATTA+TGG 0.345905 4:+6496173 MsG0480018550.01.T01:CDS
GTGAAGAGAGCAACATTATA+TGG 0.349402 4:+6496326 MsG0480018550.01.T01:CDS
CTGCCCGTATCCAAATTCTT+AGG 0.358627 4:-6495037 None:intergenic
AGGCAAAACTTAGCAGAAAC+TGG 0.372974 4:-6496374 None:intergenic
AAGAATTTGGATACGGGCAG+GGG 0.383264 4:+6495040 MsG0480018550.01.T01:CDS
AATTCTTCACAAGGATTCCT+TGG 0.390278 4:-6494884 None:intergenic
GGAATGTTGAGGGGCTGGTT+TGG 0.392660 4:-6496140 None:intergenic
TAGCTTCCTTACCATGCCAT+TGG 0.394737 4:+6495989 MsG0480018550.01.T01:CDS
AATCCCACAGCGCCTTCTCT+TGG 0.395694 4:-6496302 None:intergenic
CAATGGAACCAACATCTCTA+AGG 0.402119 4:-6495158 None:intergenic
GTACAAGAGTGTTCAGAACT+TGG 0.408992 4:+6496067 MsG0480018550.01.T01:CDS
ACTGATGCAAATTGGTTAGC+TGG 0.412013 4:-6495861 None:intergenic
TTAGAGATGTTGGTTCCATT+GGG 0.416438 4:+6495160 MsG0480018550.01.T01:CDS
AATAAGGAATGTTGAGGGGC+TGG 0.425217 4:-6496145 None:intergenic
AATTCCCCTCAAAATCTTAG+TGG 0.429720 4:+6494613 MsG0480018550.01.T01:CDS
CATTGGTGAATTTGAGGTAA+AGG 0.432182 4:+6496454 MsG0480018550.01.T01:CDS
ATCCCACAGCGCCTTCTCTT+GGG 0.434305 4:-6496301 None:intergenic
GATAAGCATTGGTGAATTTG+AGG 0.438074 4:+6496448 MsG0480018550.01.T01:CDS
AGCTTCCTATCTTTGCCACT+TGG 0.441332 4:+6494698 MsG0480018550.01.T01:CDS
GTTGAGTTCCTTAGAGATGT+TGG 0.445404 4:+6495150 MsG0480018550.01.T01:CDS
GGATTAAGTAACACAGAAGT+AGG 0.447147 4:-6496092 None:intergenic
ACTTCTGTGTTACTTAATCC+TGG 0.451739 4:+6496095 MsG0480018550.01.T01:CDS
TGGTACCAAGTGGCAAAGAT+AGG 0.458873 4:-6494703 None:intergenic
CAAAACCTTGATTGCACCTG+TGG 0.476027 4:+6494995 MsG0480018550.01.T01:CDS
CTGGTATAGCACCACAACTT+AGG 0.481431 4:+6496114 MsG0480018550.01.T01:CDS
GACTATTATCCTACAAAAGA+AGG 0.481572 4:+6496221 MsG0480018550.01.T01:CDS
TTCAAGGTCGTTGATTGCAC+TGG 0.491294 4:+6496277 MsG0480018550.01.T01:CDS
AAGGAACTCAACATGGAACT+TGG 0.499089 4:-6495139 None:intergenic
AACAAATGTTGCTGCGTCCA+AGG 0.504062 4:+6494867 MsG0480018550.01.T01:CDS
GGAGTCTTCGTTAGAGAAAG+TGG 0.504992 4:+6495079 MsG0480018550.01.T01:CDS
CCATAATAATACGTGGGTTG+TGG 0.507333 4:-6496173 None:intergenic
CTAAGTTGTGGTGCTATACC+AGG 0.515459 4:-6496113 None:intergenic
TTCTTAGGTTGTCTATTAGG+AGG 0.515531 4:-6495022 None:intergenic
TAAGAATTTGGATACGGGCA+GGG 0.516559 4:+6495039 MsG0480018550.01.T01:CDS
ATGACTCTGAGAGTTGTTGT+TGG 0.523238 4:-6494760 None:intergenic
TTGGTCATTGTTGTCAGTGG+TGG 0.527151 4:-6494741 None:intergenic
CATCTCTAAGGAACTCAACA+TGG 0.531180 4:-6495146 None:intergenic
CTAAGAATTTGGATACGGGC+AGG 0.532963 4:+6495038 MsG0480018550.01.T01:CDS
ACACATCCAGCAAAAGAGTC+AGG 0.540251 4:-6496035 None:intergenic
ACAACCTAAGAATTTGGATA+CGG 0.544621 4:+6495033 MsG0480018550.01.T01:CDS
TGGTAAAATCTTCAAATCAT+CGG 0.545386 4:-6495119 None:intergenic
CTGTAGACCATAATAATACG+TGG 0.545966 4:-6496180 None:intergenic
AGAGATATTTGATGTTGTCA+AGG 0.547142 4:-6494790 None:intergenic
CTGATGCAAATTGGTTAGCT+GGG 0.547375 4:-6495860 None:intergenic
TCTGTTCTTTCAGATATGAA+AGG 0.549535 4:-6496394 None:intergenic
TATTAAATAAGGAATGTTGA+GGG 0.558962 4:-6496150 None:intergenic
TTGTTGGTCATTGTTGTCAG+TGG 0.561480 4:-6494744 None:intergenic
ATAATCTATGCAGAAGCAAA+CGG 0.568091 4:+6495945 MsG0480018550.01.T01:CDS
TGGACCCCAATGGCATGGTA+AGG 0.574831 4:-6495995 None:intergenic
TACAATGGACCCCAATGGCA+TGG 0.580817 4:-6496000 None:intergenic
GCTTCCTTACCATGCCATTG+GGG 0.583133 4:+6495991 MsG0480018550.01.T01:CDS
GACCCAAGAGAAGGCGCTGT+GGG 0.589843 4:+6496299 MsG0480018550.01.T01:CDS
TTCATGCATAATTCTTCACA+AGG 0.597426 4:-6494893 None:intergenic
CAACCTAAGAATTTGGATAC+GGG 0.601650 4:+6495034 MsG0480018550.01.T01:CDS
GTATTAAATAAGGAATGTTG+AGG 0.602215 4:-6496151 None:intergenic
AGAATTTGGATACGGGCAGG+GGG 0.602448 4:+6495041 MsG0480018550.01.T01:CDS
TATTTGATGTTGTCAAGGAA+TGG 0.603436 4:-6494785 None:intergenic
TTCACTTCAAGATCGTCAAG+AGG 0.603459 4:-6496422 None:intergenic
TGGTTTGGACACCTAAGTTG+TGG 0.615467 4:-6496125 None:intergenic
GATTAAGTAACACAGAAGTA+GGG 0.615768 4:-6496091 None:intergenic
TTTCTGATATCATCGGGACA+AGG 0.617619 4:-6495897 None:intergenic
GAAGAAGTCACACACAACAT+TGG 0.621785 4:+6495205 MsG0480018550.01.T01:CDS
GAGAGGTGAAGATATACTTG+AGG 0.623514 4:-6495785 None:intergenic
ATTGCACTGGACCCAAGAGA+AGG 0.623682 4:+6496290 MsG0480018550.01.T01:CDS
ATTAAATAAGGAATGTTGAG+GGG 0.631161 4:-6496149 None:intergenic
AGAGCAACATTATATGGTGT+AGG 0.631695 4:+6496332 MsG0480018550.01.T01:CDS
GTCAAGTCTGAATAACCCAA+TGG 0.632708 4:-6495175 None:intergenic
AGGTGAAGATATACTTGAGG+AGG 0.634185 4:-6495782 None:intergenic
ACACAACATTGGCAAGCAAG+AGG 0.636713 4:+6495216 MsG0480018550.01.T01:CDS
AGGTCCTAAAAGCTTTACAA+TGG 0.639517 4:-6496015 None:intergenic
TCTAACGAAGACTCCACTTG+AGG 0.644921 4:-6495071 None:intergenic
GAAGTGAAAGTGATAAGCAT+TGG 0.646678 4:+6496437 MsG0480018550.01.T01:CDS
AGCTTTACAATGGACCCCAA+TGG 0.659874 4:-6496005 None:intergenic
GGACCCAAGAGAAGGCGCTG+TGG 0.662998 4:+6496298 MsG0480018550.01.T01:CDS
ATTTGTAATAGGCTCATGAG+AGG 0.663848 4:-6495802 None:intergenic
TGTAGACCATAATAATACGT+GGG 0.679137 4:-6496179 None:intergenic
GGTGGCTAAAAGGCGAACGA+TGG 0.684330 4:-6494723 None:intergenic
GTTGTTGAATGTTGATGACA+CGG 0.700176 4:+6494919 MsG0480018550.01.T01:CDS
AGGCGAACGATGGTACCAAG+TGG 0.729828 4:-6494713 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGAGAATATATTAAAAA+AGG + Chr4:6495525-6495544 MsG0480018550.01.T01:intron 10.0%
!!! TTTTTAATAGTATTTTACCT+AGG + Chr4:6495666-6495685 MsG0480018550.01.T01:intron 15.0%
!! ATTGTAAGAATAACAGTTTA+AGG - Chr4:6495731-6495750 None:intergenic 20.0%
!! CTATACAAAATGTTAAAACA+TGG - Chr4:6495754-6495773 None:intergenic 20.0%
!! TATTAAATAAGGAATGTTGA+GGG - Chr4:6496153-6496172 None:intergenic 20.0%
!!! ATGAACTAATTTTAAGTTCA+AGG + Chr4:6496261-6496280 MsG0480018550.01.T01:CDS 20.0%
!!! CCTATTACAAATACAATTTT+GGG + Chr4:6495813-6495832 MsG0480018550.01.T01:CDS 20.0%
!!! TATCTCTAAAAACATTATTG+CGG + Chr4:6495381-6495400 MsG0480018550.01.T01:intron 20.0%
! AATAAGAGAAGAAACATGTT+AGG + Chr4:6495571-6495590 MsG0480018550.01.T01:intron 25.0%
! ATCTTTATTGTGGATAAATG+CGG + Chr4:6495334-6495353 MsG0480018550.01.T01:intron 25.0%
! ATTAAATAAGGAATGTTGAG+GGG - Chr4:6496152-6496171 None:intergenic 25.0%
! CCCAAAATTGTATTTGTAAT+AGG - Chr4:6495816-6495835 None:intergenic 25.0%
! GTAAGAAAAAGTCAATGTAA+TGG - Chr4:6495247-6495266 None:intergenic 25.0%
! GTATTAAATAAGGAATGTTG+AGG - Chr4:6496154-6496173 None:intergenic 25.0%
! TAATAGACAACCTAAGAATT+TGG + Chr4:6495027-6495046 MsG0480018550.01.T01:CDS 25.0%
! TATGTTGTGAATCTTTATTG+TGG + Chr4:6495324-6495343 MsG0480018550.01.T01:intron 25.0%
! TGGTAAAATCTTCAAATCAT+CGG - Chr4:6495122-6495141 None:intergenic 25.0%
!! AAATTCTTAGGTTGTCTATT+AGG - Chr4:6495028-6495047 None:intergenic 25.0%
!! GCCTATTACAAATACAATTT+TGG + Chr4:6495812-6495831 MsG0480018550.01.T01:CDS 25.0%
!!! AAAAAATTTTTTGGGAACCT+AGG - Chr4:6495686-6495705 None:intergenic 25.0%
!!! ATCCATTTTTCTGATATCAT+CGG - Chr4:6495907-6495926 None:intergenic 25.0%
!!! ATGCTAGCCAAAAAATTTTT+TGG - Chr4:6495695-6495714 None:intergenic 25.0%
!!! GTTTTTAGAGATATTCACAT+CGG - Chr4:6495374-6495393 None:intergenic 25.0%
!!! TAGGTTCCCAAAAAATTTTT+TGG + Chr4:6495685-6495704 MsG0480018550.01.T01:intron 25.0%
!!! TGCTAGCCAAAAAATTTTTT+GGG - Chr4:6495694-6495713 None:intergenic 25.0%
AAGAAACATGTTAGGATAAG+AGG + Chr4:6495579-6495598 MsG0480018550.01.T01:intron 30.0%
ACAACCTAAGAATTTGGATA+CGG + Chr4:6495033-6495052 MsG0480018550.01.T01:CDS 30.0%
AGAGATATTTGATGTTGTCA+AGG - Chr4:6494793-6494812 None:intergenic 30.0%
AGGTTCATGGTTTAACAATA+TGG - Chr4:6495287-6495306 None:intergenic 30.0%
ATAAGTCTGCTAGTAATATC+AGG + Chr4:6495460-6495479 MsG0480018550.01.T01:intron 30.0%
ATAATCTATGCAGAAGCAAA+CGG + Chr4:6495945-6495964 MsG0480018550.01.T01:CDS 30.0%
GACTATTATCCTACAAAAGA+AGG + Chr4:6496221-6496240 MsG0480018550.01.T01:CDS 30.0%
GATTAAGTAACACAGAAGTA+GGG - Chr4:6496094-6496113 None:intergenic 30.0%
TATTTGATGTTGTCAAGGAA+TGG - Chr4:6494788-6494807 None:intergenic 30.0%
TCATAAGTTAGCATAATTGC+AGG - Chr4:6495488-6495507 None:intergenic 30.0%
TCTGTTCTTTCAGATATGAA+AGG - Chr4:6496397-6496416 None:intergenic 30.0%
TGTAGACCATAATAATACGT+GGG - Chr4:6496182-6496201 None:intergenic 30.0%
TTCATGCATAATTCTTCACA+AGG - Chr4:6494896-6494915 None:intergenic 30.0%
! AGAAAGTGGACAAAAGTTTT+TGG + Chr4:6495093-6495112 MsG0480018550.01.T01:CDS 30.0%
! AGTTGAGATTTTGGACAATT+TGG - Chr4:6494817-6494836 None:intergenic 30.0%
! GAAATCAATCCTTCTTTTGT+AGG - Chr4:6496233-6496252 None:intergenic 30.0%
! TAGGATAAGAGGTATAGTTA+GGG + Chr4:6495590-6495609 MsG0480018550.01.T01:intron 30.0%
! TTAGGATAAGAGGTATAGTT+AGG + Chr4:6495589-6495608 MsG0480018550.01.T01:intron 30.0%
! TTTGTCCACTAAGATTTTGA+GGG - Chr4:6494621-6494640 None:intergenic 30.0%
!! TCCATTTTTCTGATATCATC+GGG - Chr4:6495906-6495925 None:intergenic 30.0%
!! TGACTTTTTCTTACTTGCAT+AGG + Chr4:6495253-6495272 MsG0480018550.01.T01:intron 30.0%
!!! AAGCAACAAAGTTGTTTTTG+TGG + Chr4:6494643-6494662 MsG0480018550.01.T01:CDS 30.0%
!!! AATTTTTTGGCTAGCATTAG+TGG + Chr4:6495698-6495717 MsG0480018550.01.T01:intron 30.0%
AATTCCCCTCAAAATCTTAG+TGG + Chr4:6494613-6494632 MsG0480018550.01.T01:CDS 35.0%
AATTCTTCACAAGGATTCCT+TGG - Chr4:6494887-6494906 None:intergenic 35.0%
AGAGCAACATTATATGGTGT+AGG + Chr4:6496332-6496351 MsG0480018550.01.T01:CDS 35.0%
AGGTCCTAAAAGCTTTACAA+TGG - Chr4:6496018-6496037 None:intergenic 35.0%
AGTTAGGGAAGTTAAGTAGT+TGG + Chr4:6495605-6495624 MsG0480018550.01.T01:intron 35.0%
ATAACTGAAACCTCAAGTGA+AGG - Chr4:6495307-6495326 None:intergenic 35.0%
ATTTGTAATAGGCTCATGAG+AGG - Chr4:6495805-6495824 None:intergenic 35.0%
CAACCTAAGAATTTGGATAC+GGG + Chr4:6495034-6495053 MsG0480018550.01.T01:CDS 35.0%
CTCTAACTACTGATGCAAAT+TGG - Chr4:6495872-6495891 None:intergenic 35.0%
CTGTAGACCATAATAATACG+TGG - Chr4:6496183-6496202 None:intergenic 35.0%
GAAGTGAAAGTGATAAGCAT+TGG + Chr4:6496437-6496456 MsG0480018550.01.T01:CDS 35.0%
GGATTAAGTAACACAGAAGT+AGG - Chr4:6496095-6496114 None:intergenic 35.0%
GTGAAGAGAGCAACATTATA+TGG + Chr4:6496326-6496345 MsG0480018550.01.T01:CDS 35.0%
TAGCAGACTTATATCCCAAA+GGG - Chr4:6495452-6495471 None:intergenic 35.0%
TCCCGATGATATCAGAAAAA+TGG + Chr4:6495902-6495921 MsG0480018550.01.T01:CDS 35.0%
TCTTACTTGCATAGGATCTT+AGG + Chr4:6495261-6495280 MsG0480018550.01.T01:intron 35.0%
! CATTGGTGAATTTGAGGTAA+AGG + Chr4:6496454-6496473 MsG0480018550.01.T01:CDS 35.0%
! GTGGGTTGTGGTATTAAATA+AGG - Chr4:6496164-6496183 None:intergenic 35.0%
! GTTTGTCCACTAAGATTTTG+AGG - Chr4:6494622-6494641 None:intergenic 35.0%
! TTCTTAGGTTGTCTATTAGG+AGG - Chr4:6495025-6495044 None:intergenic 35.0%
! TTGTCCACTAAGATTTTGAG+GGG - Chr4:6494620-6494639 None:intergenic 35.0%
!! ACTTCTGTGTTACTTAATCC+TGG + Chr4:6496095-6496114 MsG0480018550.01.T01:CDS 35.0%
!! GATAAGCATTGGTGAATTTG+AGG + Chr4:6496448-6496467 MsG0480018550.01.T01:CDS 35.0%
!! GTTGTTGAATGTTGATGACA+CGG + Chr4:6494919-6494938 MsG0480018550.01.T01:CDS 35.0%
!! TTAGAGATGTTGGTTCCATT+GGG + Chr4:6495160-6495179 MsG0480018550.01.T01:CDS 35.0%
AAACCATGAACCTTCACTTG+AGG + Chr4:6495294-6495313 MsG0480018550.01.T01:intron 40.0%
AAACCTCAAGTGAAGGTTCA+TGG - Chr4:6495300-6495319 None:intergenic 40.0%
AAGGAACTCAACATGGAACT+TGG - Chr4:6495142-6495161 None:intergenic 40.0%
ACTGATGCAAATTGGTTAGC+TGG - Chr4:6495864-6495883 None:intergenic 40.0%
AGAACTATGCTTCACCCTTT+GGG + Chr4:6495435-6495454 MsG0480018550.01.T01:intron 40.0%
AGGCAAAACTTAGCAGAAAC+TGG - Chr4:6496377-6496396 None:intergenic 40.0%
AGGTGAAGATATACTTGAGG+AGG - Chr4:6495785-6495804 None:intergenic 40.0%
ATCTCAACTCAGACGATGTT+TGG + Chr4:6494828-6494847 MsG0480018550.01.T01:CDS 40.0%
CAATGGAACCAACATCTCTA+AGG - Chr4:6495161-6495180 None:intergenic 40.0%
CCACAACCCACGTATTATTA+TGG + Chr4:6496173-6496192 MsG0480018550.01.T01:CDS 40.0%
CTAGCAGACTTATATCCCAA+AGG - Chr4:6495453-6495472 None:intergenic 40.0%
GAAGAAGTCACACACAACAT+TGG + Chr4:6495205-6495224 MsG0480018550.01.T01:CDS 40.0%
GAGAGGTGAAGATATACTTG+AGG - Chr4:6495788-6495807 None:intergenic 40.0%
GTCAAGTCTGAATAACCCAA+TGG - Chr4:6495178-6495197 None:intergenic 40.0%
GTTGAGTTCCTTAGAGATGT+TGG + Chr4:6495150-6495169 MsG0480018550.01.T01:CDS 40.0%
TAAGAATTTGGATACGGGCA+GGG + Chr4:6495039-6495058 MsG0480018550.01.T01:CDS 40.0%
TAGAACTATGCTTCACCCTT+TGG + Chr4:6495434-6495453 MsG0480018550.01.T01:intron 40.0%
TAGGGAAGTTAAGTAGTTGG+AGG + Chr4:6495608-6495627 MsG0480018550.01.T01:intron 40.0%
TTCACTTCAAGATCGTCAAG+AGG - Chr4:6496425-6496444 None:intergenic 40.0%
TTGTTGGTCATTGTTGTCAG+TGG - Chr4:6494747-6494766 None:intergenic 40.0%
TTTCTGATATCATCGGGACA+AGG - Chr4:6495900-6495919 None:intergenic 40.0%
! ATGACTCTGAGAGTTGTTGT+TGG - Chr4:6494763-6494782 None:intergenic 40.0%
! CATCTCTAAGGAACTCAACA+TGG - Chr4:6495149-6495168 None:intergenic 40.0%
! CCATAATAATACGTGGGTTG+TGG - Chr4:6496176-6496195 None:intergenic 40.0%
! CTGATGCAAATTGGTTAGCT+GGG - Chr4:6495863-6495882 None:intergenic 40.0%
! GGGTCCATTGTAAAGCTTTT+AGG + Chr4:6496011-6496030 MsG0480018550.01.T01:CDS 40.0%
! TCTATTAGGAGGTTTTCCAC+AGG - Chr4:6495014-6495033 None:intergenic 40.0%
!! CATCGTCTGAGTTGAGATTT+TGG - Chr4:6494826-6494845 None:intergenic 40.0%
!! CTTAGAGATGTTGGTTCCAT+TGG + Chr4:6495159-6495178 MsG0480018550.01.T01:CDS 40.0%
!! GTACAAGAGTGTTCAGAACT+TGG + Chr4:6496067-6496086 MsG0480018550.01.T01:CDS 40.0%
!! TTTTGGCTAGCATTAGTGGT+GGG + Chr4:6495702-6495721 MsG0480018550.01.T01:intron 40.0%
!!! TTTTTGGCTAGCATTAGTGG+TGG + Chr4:6495701-6495720 MsG0480018550.01.T01:intron 40.0%
AACAAATGTTGCTGCGTCCA+AGG + Chr4:6494867-6494886 MsG0480018550.01.T01:CDS 45.0%
AAGAATTTGGATACGGGCAG+GGG + Chr4:6495040-6495059 MsG0480018550.01.T01:CDS 45.0%
AATAAGGAATGTTGAGGGGC+TGG - Chr4:6496148-6496167 None:intergenic 45.0%
ACACAACATTGGCAAGCAAG+AGG + Chr4:6495216-6495235 MsG0480018550.01.T01:CDS 45.0%
ACACATCCAGCAAAAGAGTC+AGG - Chr4:6496038-6496057 None:intergenic 45.0%
AGCTTCCTATCTTTGCCACT+TGG + Chr4:6494698-6494717 MsG0480018550.01.T01:CDS 45.0%
AGCTTCCTTACCATGCCATT+GGG + Chr4:6495990-6496009 MsG0480018550.01.T01:CDS 45.0%
AGCTTTACAATGGACCCCAA+TGG - Chr4:6496008-6496027 None:intergenic 45.0%
CAAAACCTTGATTGCACCTG+TGG + Chr4:6494995-6495014 MsG0480018550.01.T01:CDS 45.0%
CTAAGAATTTGGATACGGGC+AGG + Chr4:6495038-6495057 MsG0480018550.01.T01:CDS 45.0%
CTGCCCGTATCCAAATTCTT+AGG - Chr4:6495040-6495059 None:intergenic 45.0%
CTGGTATAGCACCACAACTT+AGG + Chr4:6496114-6496133 MsG0480018550.01.T01:CDS 45.0%
GGAGTCTTCGTTAGAGAAAG+TGG + Chr4:6495079-6495098 MsG0480018550.01.T01:CDS 45.0%
TAGCTTCCTTACCATGCCAT+TGG + Chr4:6495989-6496008 MsG0480018550.01.T01:CDS 45.0%
TCTAACGAAGACTCCACTTG+AGG - Chr4:6495074-6495093 None:intergenic 45.0%
TGGTACCAAGTGGCAAAGAT+AGG - Chr4:6494706-6494725 None:intergenic 45.0%
TGTTGTCAGTGGTGGCTAAA+AGG - Chr4:6494736-6494755 None:intergenic 45.0%
TTGGTCATTGTTGTCAGTGG+TGG - Chr4:6494744-6494763 None:intergenic 45.0%
! GACTCTTTTGCTGGATGTGT+TGG + Chr4:6496038-6496057 MsG0480018550.01.T01:CDS 45.0%
! GTTTTCCACAGGTGCAATCA+AGG - Chr4:6495003-6495022 None:intergenic 45.0%
! TGGTTTGGACACCTAAGTTG+TGG - Chr4:6496128-6496147 None:intergenic 45.0%
! TTAGGACCTGACTCTTTTGC+TGG + Chr4:6496029-6496048 MsG0480018550.01.T01:CDS 45.0%
! TTCAAGGTCGTTGATTGCAC+TGG + Chr4:6496277-6496296 MsG0480018550.01.T01:CDS 45.0%
!! CTAAGTTGTGGTGCTATACC+AGG - Chr4:6496116-6496135 None:intergenic 45.0%
AGAATTTGGATACGGGCAGG+GGG + Chr4:6495041-6495060 MsG0480018550.01.T01:CDS 50.0%
AGGGGGAATTATGCCTCAAG+TGG + Chr4:6495058-6495077 MsG0480018550.01.T01:CDS 50.0%
ATTGCACTGGACCCAAGAGA+AGG + Chr4:6496290-6496309 MsG0480018550.01.T01:CDS 50.0%
ATTGTGGATAAATGCGGCCG+TGG + Chr4:6495340-6495359 MsG0480018550.01.T01:intron 50.0%
GCTTCCTTACCATGCCATTG+GGG + Chr4:6495991-6496010 MsG0480018550.01.T01:CDS 50.0%
TACAATGGACCCCAATGGCA+TGG - Chr4:6496003-6496022 None:intergenic 50.0%
TCACATCGGCATTGCAACCA+CGG - Chr4:6495360-6495379 None:intergenic 50.0%
! TGCGGCTGCAACTGTGTTTT+TGG + Chr4:6495399-6495418 MsG0480018550.01.T01:intron 50.0%
AATCCCACAGCGCCTTCTCT+TGG - Chr4:6496305-6496324 None:intergenic 55.0%
AGGCGAACGATGGTACCAAG+TGG - Chr4:6494716-6494735 None:intergenic 55.0%
ATCCCACAGCGCCTTCTCTT+GGG - Chr4:6496304-6496323 None:intergenic 55.0%
GGTGGCTAAAAGGCGAACGA+TGG - Chr4:6494726-6494745 None:intergenic 55.0%
TGGACCCCAATGGCATGGTA+AGG - Chr4:6495998-6496017 None:intergenic 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG - Chr4:6496143-6496162 None:intergenic 55.0%
!! GACCCAAGAGAAGGCGCTGT+GGG + Chr4:6496299-6496318 MsG0480018550.01.T01:CDS 60.0%
!! GGACCCAAGAGAAGGCGCTG+TGG + Chr4:6496298-6496317 MsG0480018550.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 6494578 6496496 6494578 ID=MsG0480018550.01;Name=MsG0480018550.01
Chr4 mRNA 6494578 6496496 6494578 ID=MsG0480018550.01.T01;Parent=MsG0480018550.01;Name=MsG0480018550.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|460
Chr4 exon 6494578 6495237 6494578 ID=MsG0480018550.01.T01:exon:27268;Parent=MsG0480018550.01.T01
Chr4 exon 6495774 6496496 6495774 ID=MsG0480018550.01.T01:exon:27269;Parent=MsG0480018550.01.T01
Chr4 CDS 6494578 6495237 6494578 ID=MsG0480018550.01.T01:cds;Parent=MsG0480018550.01.T01
Chr4 CDS 6495774 6496496 6495774 ID=MsG0480018550.01.T01:cds;Parent=MsG0480018550.01.T01
Gene Sequence

>MsG0480018550.01.T01

ATGGCTTATCGACGACAACAAATATTGTCAAAAACAATTCCCCTCAAAATCTTAGTGGACAAACAAAGCAACAAAGTTGTTTTTGTGGAAGCAATAAAAGACTTTGTTGATACTCTTTTCAGCTTCCTATCTTTGCCACTTGGTACCATCGTTCGCCTTTTAGCCACCACTGACAACAATGACCAACAACAACTCTCAGAGTCATCACCATTCCTTGACAACATCAAATATCTCTACCAAATTGTCCAAAATCTCAACTCAGACGATGTTTGGAACAACTCTGTGTGCAAACAAATGTTGCTGCGTCCAAGGAATCCTTGTGAAGAATTATGCATGAAGCTGTTGTTGAATGTTGATGACACGGAGACATCAAGTAAATTTTATGTGTGTGATTCTTGTTACAAGTTCACTAATTTTCAAAACCTTGATTGCACCTGTGGAAAACCTCCTAATAGACAACCTAAGAATTTGGATACGGGCAGGGGGAATTATGCCTCAAGTGGAGTCTTCGTTAGAGAAAGTGGACAAAAGTTTTTGGTGTCCGATGATTTGAAGATTTTACCAAGTTCCATGTTGAGTTCCTTAGAGATGTTGGTTCCATTGGGTTATTCAGACTTGACTGAACTAGAAGAAGTCACACACAACATTGGCAAGCAAGAGATATTGAACCTCCTCAAGTATATCTTCACCTCTCATGAGCCTATTACAAATACAATTTTGGGAAGTAGCTCAAACAACAGAAACAACCCAGCTAACCAATTTGCATCAGTAGTTAGAGTAACACCTTGTCCCGATGATATCAGAAAAATGGATGTTAAGTCTCAGAAAAATATAATCTATGCAGAAGCAAACGGTGATTTTGTTGACTTCATTTTTAGCTTCCTTACCATGCCATTGGGGTCCATTGTAAAGCTTTTAGGACCTGACTCTTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTTCAGAACTTGGACCCTACTTCTGTGTTACTTAATCCTGGTATAGCACCACAACTTAGGTGTCCAAACCAGCCCCTCAACATTCCTTATTTAATACCACAACCCACGTATTATTATGGTCTACAGAAAAATGATGATCACTATGACTATTATCCTACAAAAGAAGGATTGATTTCTAAGTCATATGAACTAATTTTAAGTTCAAGGTCGTTGATTGCACTGGACCCAAGAGAAGGCGCTGTGGGATTTGTGAAGAGAGCAACATTATATGGTGTAGGAGATGATCTAAAAGTAAAACCAGTTTCTGCTAAGTTTTGCCTTTCATATCTGAAAGAACAGAATCTTCCTCTTGACGATCTTGAAGTGAAAGTGATAAGCATTGGTGAATTTGAGGTAAAGGTTGCTCTTATATTAGACTAA
Protein sequence

>MsG0480018550.01.T01

MAYRRQQILSKTIPLKILVDKQSNKVVFVEAIKDFVDTLFSFLSLPLGTIVRLLATTDNNDQQQLSESSPFLDNIKYLYQIVQNLNSDDVWNNSVCKQMLLRPRNPCEELCMKLLLNVDDTETSSKFYVCDSCYKFTNFQNLDCTCGKPPNRQPKNLDTGRGNYASSGVFVRESGQKFLVSDDLKILPSSMLSSLEMLVPLGYSDLTELEEVTHNIGKQEILNLLKYIFTSHEPITNTILGSSSNNRNNPANQFASVVRVTPCPDDIRKMDVKSQKNIIYAEANGDFVDFIFSFLTMPLGSIVKLLGPDSFAGCVGNLYKSVQNLDPTSVLLNPGIAPQLRCPNQPLNIPYLIPQPTYYYGLQKNDDHYDYYPTKEGLISKSYELILSSRSLIALDPREGAVGFVKRATLYGVGDDLKVKPVSAKFCLSYLKEQNLPLDDLEVKVISIGEFEVKVALILD*