AlfalfaGEDB | MsG0480022224.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022224.01

KEGG: K24983
GO: GO:0003676
PFAM: PF00076 PF02136

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022224.01.T01	XP_024638145.1	69.526	443	26	7	1	334	1	443	0	536

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022224.01.T01	Q9FME2	30.989	455	201	14	1	360	1	437	1.09E-28	119

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022224.01.T01	G7JHJ0	69.526	443	26	7	1	334	1	443	0.0	536

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001169.01	MsG0480022224.01	0.828540	8.494191e-55	8.468201e-52
MsG0180003623.01	MsG0480022224.01	0.802338	5.881740e-49	2.873951e-46
MsG0180004503.01	MsG0480022224.01	0.802908	4.488290e-49	2.225075e-46
MsG0280006301.01	MsG0480022224.01	0.817063	4.005353e-52	2.887135e-49
MsG0380016805.01	MsG0480022224.01	0.868578	5.883109e-66	2.139033e-62
MsG0380017156.01	MsG0480022224.01	0.802030	6.806789e-49	3.299796e-46
MsG0480022224.01	MsG0480023996.01	0.821394	4.133320e-53	3.358586e-50
MsG0480022224.01	MsG0580028601.01	0.804132	2.501839e-49	1.279827e-46
MsG0480022224.01	MsG0780036859.01	0.800366	1.490702e-48	6.928607e-46
MsG0480022224.01	MsG0780040054.01	0.830395	3.008288e-55	3.165507e-52

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022224.01.T01	MTR_4g083150	69.526	443	26	7	1	334	1	443	0.0	536
MsG0480022224.01.T01	MTR_7g013840	34.916	358	164	12	9	301	11	364	2.48e-45	162
MsG0480022224.01.T01	MTR_7g013840	34.819	359	164	12	9	301	11	365	3.58e-45	162
MsG0480022224.01.T01	MTR_4g109640	34.605	367	166	11	1	302	1	358	2.39e-43	154
MsG0480022224.01.T01	MTR_4g109640	34.605	367	166	11	1	302	1	358	3.59e-43	154
MsG0480022224.01.T01	MTR_4g109640	34.605	367	166	11	1	302	1	358	1.03e-42	155
MsG0480022224.01.T01	MTR_7g057520	30.357	448	204	15	1	345	1	443	6.50e-39	145
MsG0480022224.01.T01	MTR_7g055610	30.357	448	204	15	1	345	8	450	7.94e-39	145

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022224.01.T01	AT1G13730	32.632	380	186	13	15	338	15	380	3.18e-41	150
MsG0480022224.01.T01	AT1G13730	32.632	380	186	13	15	338	15	380	3.18e-41	150
MsG0480022224.01.T01	AT3G25150	30.750	400	189	14	3	322	9	400	9.18e-40	147
MsG0480022224.01.T01	AT3G25150	30.693	404	192	14	3	326	108	503	1.97e-39	148
MsG0480022224.01.T01	AT2G03640	30.123	405	175	15	13	328	13	398	6.26e-37	139
MsG0480022224.01.T01	AT2G03640	29.557	406	175	16	13	328	13	397	6.17e-36	136
MsG0480022224.01.T01	AT2G03640	29.557	406	175	16	13	328	13	397	6.17e-36	136
MsG0480022224.01.T01	AT2G03640	29.557	406	175	16	13	328	13	397	6.17e-36	136
MsG0480022224.01.T01	AT2G03640	30.973	339	164	12	13	302	13	330	1.75e-35	135
MsG0480022224.01.T01	AT1G69250	30.294	340	180	10	10	302	8	337	1.28e-32	127

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Find 124 sgRNAs with CRISPR-Local

Find 295 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTATGAACCTGATCC+GGG	0.522821	4:-69864180	None:intergenic
AAAGAGAGAAACAAAGAATG+CGG	0.683376	4:-69863228	None:intergenic
AAAGATCACAGAAACTACAA+AGG	0.657053	4:-69867523	None:intergenic
AACAGTAGAGCAATTGGATC+GGG	0.513751	4:+69866424	MsG0480022224.01.T01:CDS
AACGGAAGGAACGATTTGAG+AGG	0.613863	4:+69867272	MsG0480022224.01.T01:CDS
AACTTTGGTGGCGGTCGTGG+GGG	0.442248	4:+69867242	MsG0480022224.01.T01:CDS
AAGAATGCGGCTGATGAGTG+AGG	0.705213	4:-69863215	None:intergenic
AAGGAACGATTTGAGAGGCG+AGG	0.663896	4:+69867277	MsG0480022224.01.T01:CDS
AAGTCATCATGTTAAGGAAC+CGG	0.473889	4:+69865943	MsG0480022224.01.T01:CDS
AATGATAACTTCAGGGGCCG+TGG	0.521415	4:+69867218	MsG0480022224.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAGAGAAACAAAGAATG+CGG	-	Chr4:69863231-69863250	None:intergenic	30.0%
AAAGATCACAGAAACTACAA+AGG	-	Chr4:69867526-69867545	None:intergenic	30.0%
AATCAAAAACACACAAACAC+GGG	-	Chr4:69864018-69864037	None:intergenic	30.0%
ACTAGAAAGTTAGATACAGT+AGG	-	Chr4:69867630-69867649	None:intergenic	30.0%
ACTGCAGTAAGTTCAAAAAA+TGG	+	Chr4:69864265-69864284	MsG0480022224.01.T01:intron	30.0%
AGTTACGCAATTTACTACTT+TGG	-	Chr4:69864388-69864407	None:intergenic	30.0%
ATAGAAATACTCATACCTCT+AGG	-	Chr4:69866648-69866667	None:intergenic	30.0%
ATATACAAGTATAGAATGGC+AGG	-	Chr4:69867130-69867149	None:intergenic	30.0%
ATCAATAGTTGTTATCAGCA+AGG	+	Chr4:69866666-69866685	MsG0480022224.01.T01:intron	30.0%
ATGAACTTGAACCGTAAAAA+GGG	-	Chr4:69864980-69864999	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	69863167	69867698	69863167	ID=MsG0480022224.01;Name=MsG0480022224.01
Chr4	mRNA	69863167	69867698	69863167	ID=MsG0480022224.01.T01;Parent=MsG0480022224.01;Name=MsG0480022224.01.T01;_AED=0.31;_eAED=0.31;_QI=122\|0.62\|0.55\|1\|0.87\|0.77\|9\|283\|376
Chr4	exon	69863167	69863286	69863167	ID=MsG0480022224.01.T01:exon:59603;Parent=MsG0480022224.01.T01
Chr4	exon	69864089	69864270	69864089	ID=MsG0480022224.01.T01:exon:59604;Parent=MsG0480022224.01.T01
Chr4	exon	69865124	69865225	69865124	ID=MsG0480022224.01.T01:exon:59605;Parent=MsG0480022224.01.T01
Chr4	exon	69865333	69865429	69865333	ID=MsG0480022224.01.T01:exon:59606;Parent=MsG0480022224.01.T01
Chr4	exon	69865750	69866038	69865750	ID=MsG0480022224.01.T01:exon:59607;Parent=MsG0480022224.01.T01
Chr4	exon	69866202	69866269	69866202	ID=MsG0480022224.01.T01:exon:59608;Parent=MsG0480022224.01.T01
Chr4	exon	69866381	69866502	69866381	ID=MsG0480022224.01.T01:exon:59609;Parent=MsG0480022224.01.T01
Chr4	exon	69866593	69866641	69866593	ID=MsG0480022224.01.T01:exon:59610;Parent=MsG0480022224.01.T01
Chr4	exon	69867192	69867698	69867192	ID=MsG0480022224.01.T01:exon:59611;Parent=MsG0480022224.01.T01
Chr4	five_prime_UTR	69863167	69863286	69863167	ID=MsG0480022224.01.T01:five_prime_utr;Parent=MsG0480022224.01.T01
Chr4	five_prime_UTR	69864089	69864090	69864089	ID=MsG0480022224.01.T01:five_prime_utr;Parent=MsG0480022224.01.T01
Chr4	CDS	69864091	69864270	69864091	ID=MsG0480022224.01.T01:cds;Parent=MsG0480022224.01.T01
Chr4	CDS	69865124	69865225	69865124	ID=MsG0480022224.01.T01:cds;Parent=MsG0480022224.01.T01
Chr4	CDS	69865333	69865429	69865333	ID=MsG0480022224.01.T01:cds;Parent=MsG0480022224.01.T01
Chr4	CDS	69865750	69866038	69865750	ID=MsG0480022224.01.T01:cds;Parent=MsG0480022224.01.T01
Chr4	CDS	69866202	69866269	69866202	ID=MsG0480022224.01.T01:cds;Parent=MsG0480022224.01.T01
Chr4	CDS	69866381	69866502	69866381	ID=MsG0480022224.01.T01:cds;Parent=MsG0480022224.01.T01
Chr4	CDS	69866593	69866641	69866593	ID=MsG0480022224.01.T01:cds;Parent=MsG0480022224.01.T01
Chr4	CDS	69867192	69867415	69867192	ID=MsG0480022224.01.T01:cds;Parent=MsG0480022224.01.T01
Chr4	three_prime_UTR	69867416	69867698	69867416	ID=MsG0480022224.01.T01:three_prime_utr;Parent=MsG0480022224.01.T01

Gene Sequence

>MsG0480022224.01.T01

ATGGCGGTACCTGAAGCAGTTCAAACCCCAACCTCTCAGATGGTTGGCAATGCTTTTGTGGAGCAGTATTACTCAATTCTGCACCGCGACCCGGATCAGGTTCATAGGTTTTACCACGACTCAAGTGTCATGAGTCGACCTGAAGAAGATGGTACCATGACAACTGTCACCACCACTGCAGAAATTGATAAAAAGATACAATCTCTCAAGTACACAAGCTTTAGGGTGGAGGTTTTGAGTGCTGATGCTCAGCCTTCATATAATAATGGAGTGATGGTTGTAATATGTGATGCGTATAAGTCAATTGATACCGAGTCTGTGCCAGCAAATGATGCTGATGAAAGTGCTCCATCAGAACCTATTATTACACCCGAGCCTGAGCCTGTTCATGTTCCTGAAGTCATTCCACCCACTCAAACTGTTGTTCCACCCGCTCAAACTGTTATTCCACCCACTCAAACTGTTATTGCTGATACTGAAACTATCATCAGCAAAGAAGTGAGCTTGCCACTGGAGAATGGAAAATTATCAGTTACTGAAAATGTGATTCCTGTTAATCATGTTAAAGAGTCAAGTCATCATGTTAAGGAACCGGAACAACCCACAAGCATTGAGAAAGTTGCTTCAAATACACAGGAGGATACTCCAAAAAAGTCTTTGCATCCATTCGTGGCTGCTCCTGAAGTACCAGCCCCTAACAATGACATTCCATTGGAAAAGAATAATGAGAACGCAGGTAGGGCTCATGCAATATTTGTTGCGAATTTGCCTATGAGTGCAACAGTAGAGCAATTGGATCGGGCTTTCAAGAAATTTGGGCCCATTAAACGTGATGGTATTCAAGTTAGAAGTAACAAGGGGTCTTGTTTTGGTTTTGTGGAGTTTGAATCTGCTGCTTCAATGCAAAGGCGTAGTGGATACCGAAATGACAGAAATGATAACTTCAGGGGCCGTGGCAACTTTGGTGGCGGTCGTGGGGGTGGCTTTAACGGAAGGAACGATTTGAGAGGCGAGGTGAGTTCTCTGGCCGACCTCGAGGAGGCCAGAATGCCGGGCGAAGCAATGGAGATGCTGTGCCAAGGAGTTATCAGAATGGAGGAGGAAAAGTCGCTGCTCGTCAACCACCAGTGA

Protein sequence

>MsG0480022224.01.T01

MAVPEAVQTPTSQMVGNAFVEQYYSILHRDPDQVHRFYHDSSVMSRPEEDGTMTTVTTTAEIDKKIQSLKYTSFRVEVLSADAQPSYNNGVMVVICDAYKSIDTESVPANDADESAPSEPIITPEPEPVHVPEVIPPTQTVVPPAQTVIPPTQTVIADTETIISKEVSLPLENGKLSVTENVIPVNHVKESSHHVKEPEQPTSIEKVASNTQEDTPKKSLHPFVAAPEVPAPNNDIPLEKNNENAGRAHAIFVANLPMSATVEQLDRAFKKFGPIKRDGIQVRSNKGSCFGFVEFESAASMQRRSGYRNDRNDNFRGRGNFGGGRGGGFNGRNDLRGEVSSLADLEEARMPGEAMEMLCQGVIRMEEEKSLLVNHQ*