AlfalfaGEDB | MsG0480023855.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023855.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023855.01.T01	AET04117.1	96.512	86	1	2	3	86	17	102	1.83E-49	161

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023855.01.T01	G7LH58	96.512	86	1	2	3	86	17	102	8.75e-50	161

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000138.01	MsG0480023855.01	0.818253	2.158654e-52	1.607533e-49
MsG0180004942.01	MsG0480023855.01	0.811914	5.516309e-51	3.457732e-48
MsG0180005102.01	MsG0480023855.01	0.821803	3.325033e-53	2.733123e-50
MsG0480019017.01	MsG0480023855.01	0.808798	2.595135e-50	1.497777e-47
MsG0480020814.01	MsG0480023855.01	0.805267	1.449360e-49	7.634097e-47
MsG0480022637.01	MsG0480023855.01	0.850124	2.079161e-60	4.030967e-57
MsG0480023855.01	MsG0680034316.01	0.801167	1.023287e-48	4.853584e-46
MsG0480023855.01	MsG0780036370.01	0.807177	5.741275e-50	3.176169e-47
MsG0480023855.01	MsG0780038860.01	0.807259	5.515168e-50	3.057677e-47
MsG0480023855.01	MsG0780039190.01	0.811095	8.311019e-51	5.098250e-48
MsG0480023855.01	MsG0780040977.01	0.800853	1.185894e-48	5.580526e-46
MsG0480023855.01	MsG0880046174.01	-0.818390	2.010199e-52	1.502783e-49
MsG0380016476.01	MsG0480023855.01	0.827758	1.310907e-54	1.277310e-51
MsG0480018440.01	MsG0480023855.01	0.809182	2.147497e-50	1.252287e-47
MsG0280006853.01	MsG0480023855.01	0.817122	3.883625e-52	2.803827e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023855.01.T01	MTR_8g083450	96.512	86	1	2	3	86	17	102	2.22e-53	161
MsG0480023855.01.T01	MTR_6g086485	61.111	90	29	4	1	86	1	88	2.43e-24	87.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGACACATGAAGGGTCTTTC+AGG	0.198905	4:+90516618	MsG0480023855.01.T01:CDS
GCCGATTCCACTGTCACTTT+TGG	0.208493	4:-90516549	None:intergenic
GCTTAATTTGAGGTTGAGAT+TGG	0.382215	4:-90516505	None:intergenic
ACAACAGACTTATTATGATT+AGG	0.402368	4:-90516716	None:intergenic
GAACATAGACACCGTCCTTT+GGG	0.403670	4:-90516590	None:intergenic
ATGATTAGGAGGCATGGATT+TGG	0.406829	4:-90516702	None:intergenic
TGAACATAGACACCGTCCTT+TGG	0.409239	4:-90516591	None:intergenic
CACCTCCACCTTGGATGAAA+AGG	0.419918	4:+90516678	MsG0480023855.01.T01:CDS
CCCAAAAGTGACAGTGGAAT+CGG	0.461245	4:+90516548	MsG0480023855.01.T01:CDS
CTTAATTTGAGGTTGAGATT+GGG	0.479480	4:-90516504	None:intergenic
CTTATTATGATTAGGAGGCA+TGG	0.481755	4:-90516708	None:intergenic
CGTCATCTGCACCTCCACCT+TGG	0.488683	4:+90516669	MsG0480023855.01.T01:CDS
TGTTCACCAACGACACATGA+AGG	0.528018	4:+90516608	MsG0480023855.01.T01:CDS
GAAACGATGAGCTTAATTTG+AGG	0.569661	4:-90516515	None:intergenic
ACAGACTTATTATGATTAGG+AGG	0.572228	4:-90516713	None:intergenic
AAAGACCCTTCATGTGTCGT+TGG	0.628957	4:-90516614	None:intergenic
GCAGCAGCGTCGTTTCCCAA+AGG	0.634028	4:+90516575	MsG0480023855.01.T01:CDS
CAGCGTCGTTTCCCAAAGGA+CGG	0.634645	4:+90516579	MsG0480023855.01.T01:CDS
GTTCACCAACGACACATGAA+GGG	0.648482	4:+90516609	MsG0480023855.01.T01:CDS
TCATCACCCAAAAGTGACAG+TGG	0.653040	4:+90516542	MsG0480023855.01.T01:CDS
AAACATATAGTAACTCACTG+TGG	0.708803	4:-90516749	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACAACAGACTTATTATGATT+AGG	-	Chr4:90516719-90516738	None:intergenic	25.0%
	ACAGACTTATTATGATTAGG+AGG	-	Chr4:90516716-90516735	None:intergenic	30.0%
!	CTTAATTTGAGGTTGAGATT+GGG	-	Chr4:90516507-90516526	None:intergenic	30.0%
	CTTATTATGATTAGGAGGCA+TGG	-	Chr4:90516711-90516730	None:intergenic	35.0%
!	GAAACGATGAGCTTAATTTG+AGG	-	Chr4:90516518-90516537	None:intergenic	35.0%
!	GCTTAATTTGAGGTTGAGAT+TGG	-	Chr4:90516508-90516527	None:intergenic	35.0%
!	ATGATTAGGAGGCATGGATT+TGG	-	Chr4:90516705-90516724	None:intergenic	40.0%
!!	GATTTGGTCCTTTTCATCCA+AGG	-	Chr4:90516689-90516708	None:intergenic	40.0%
	AAAGACCCTTCATGTGTCGT+TGG	-	Chr4:90516617-90516636	None:intergenic	45.0%
	CCCAAAAGTGACAGTGGAAT+CGG	+	Chr4:90516548-90516567	MsG0480023855.01.T01:CDS	45.0%
	GAACATAGACACCGTCCTTT+GGG	-	Chr4:90516593-90516612	None:intergenic	45.0%
	GTTCACCAACGACACATGAA+GGG	+	Chr4:90516609-90516628	MsG0480023855.01.T01:CDS	45.0%
	TCATCACCCAAAAGTGACAG+TGG	+	Chr4:90516542-90516561	MsG0480023855.01.T01:CDS	45.0%
	TGAACATAGACACCGTCCTT+TGG	-	Chr4:90516594-90516613	None:intergenic	45.0%
	TGTTCACCAACGACACATGA+AGG	+	Chr4:90516608-90516627	MsG0480023855.01.T01:CDS	45.0%
!	TTGGTCCTTTTCATCCAAGG+TGG	-	Chr4:90516686-90516705	None:intergenic	45.0%
!!!	AGGTGTCGTTTTCATCGATC+AGG	+	Chr4:90516638-90516657	MsG0480023855.01.T01:CDS	45.0%
!!!	CCGATTCCACTGTCACTTTT+GGG	-	Chr4:90516551-90516570	None:intergenic	45.0%
!!!	GGTGTCGTTTTCATCGATCA+GGG	+	Chr4:90516639-90516658	MsG0480023855.01.T01:CDS	45.0%
	CACCTCCACCTTGGATGAAA+AGG	+	Chr4:90516678-90516697	MsG0480023855.01.T01:CDS	50.0%
	CGACACATGAAGGGTCTTTC+AGG	+	Chr4:90516618-90516637	MsG0480023855.01.T01:CDS	50.0%
!	GTCCTTTTCATCCAAGGTGG+AGG	-	Chr4:90516683-90516702	None:intergenic	50.0%
!!!	GCCGATTCCACTGTCACTTT+TGG	-	Chr4:90516552-90516571	None:intergenic	50.0%
	CAGCGTCGTTTCCCAAAGGA+CGG	+	Chr4:90516579-90516598	MsG0480023855.01.T01:CDS	55.0%
	CGTCATCTGCACCTCCACCT+TGG	+	Chr4:90516669-90516688	MsG0480023855.01.T01:CDS	60.0%
	GCAGCAGCGTCGTTTCCCAA+AGG	+	Chr4:90516575-90516594	MsG0480023855.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	90516497	90516757	90516497	ID=MsG0480023855.01;Name=MsG0480023855.01
Chr4	mRNA	90516497	90516757	90516497	ID=MsG0480023855.01.T01;Parent=MsG0480023855.01;Name=MsG0480023855.01.T01;_AED=0.38;_eAED=0.38;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|86
Chr4	exon	90516497	90516757	90516497	ID=MsG0480023855.01.T01:exon:15235;Parent=MsG0480023855.01.T01
Chr4	CDS	90516497	90516757	90516497	ID=MsG0480023855.01.T01:cds;Parent=MsG0480023855.01.T01
Chr4	mRNA	90516573	90516695	90516573	ID=MsG0480023855.01.T02;Parent=MsG0480023855.01;Name=MsG0480023855.01.T02;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|40
Chr4	exon	90516573	90516695	90516573	ID=MsG0480023855.01.T02:exon:15236;Parent=MsG0480023855.01.T02
Chr4	CDS	90516573	90516695	90516573	ID=MsG0480023855.01.T02:cds;Parent=MsG0480023855.01.T02

Gene Sequence

>MsG0480023855.01.T02

ATGCAGCAGCGTCGTTTCCCAAAGGACGGTGTCTATGTTCACCAACGACACATGAAGGGTCTTTCAGGTGTCGTTTTCATCGATCAGGGACATTTTCGTCATCTGCACCTCCACCTTGGATGA

>MsG0480023855.01.T01

ATGGCTTCCCAATCTCAACCTCAAATTAAGCTCATCGTTTCAACATCATCACCCAAAAGTGACAGTGGAATCGGCGATGCAGCAGCGTCGTTTCCCAAAGGACGGTGTCTATGTTCACCAACGACACATGAAGGGTCTTTCAGGTGTCGTTTTCATCGATCAGGGACATTTTCGTCATCTGCACCTCCACCTTGGATGAAAAGGACCAAATCCATGCCTCCTAATCATAATAAGTCTGTTGTTTCTGTTTCTCCACAGTGA

Protein sequence

>MsG0480023855.01.T02

MQQRRFPKDGVYVHQRHMKGLSGVVFIDQGHFRHLHLHLG*

>MsG0480023855.01.T01

MASQSQPQIKLIVSTSSPKSDSGIGDAAASFPKGRCLCSPTTHEGSFRCRFHRSGTFSSSAPPPWMKRTKSMPPNHNKSVVSVSPQ*