AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039190.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039190.01.T01 MTR_7g067510 96.867 415 13 0 1 415 33 447 0.0 830
MsG0780039190.01.T01 MTR_2g013300 53.512 299 135 1 105 399 124 422 4.86e-106 320
MsG0780039190.01.T01 MTR_4g084060 32.333 300 180 7 123 413 287 572 3.14e-40 151
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039190.01.T01 AT2G38150 54.294 326 147 2 89 413 73 397 4.21e-130 380
MsG0780039190.01.T01 AT5G01250 56.494 308 133 1 108 415 96 402 5.79e-126 370
MsG0780039190.01.T01 AT3G09020 53.106 322 148 2 94 413 84 404 1.34e-124 367
MsG0780039190.01.T01 AT2G38152 48.148 351 150 3 65 414 57 376 2.44e-117 347
MsG0780039190.01.T01 AT1G61050 54.698 298 132 2 118 413 132 428 2.32e-109 329
MsG0780039190.01.T01 AT1G61050 54.698 298 132 2 118 413 131 427 2.35e-109 329
MsG0780039190.01.T01 AT4G19900 32.667 300 179 8 123 413 355 640 1.68e-41 155

Find 60 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTATGTTGAGATTTAA+TGG 0.162859 7:+59445924 None:intergenic
CTCAAACCATTCATTGATAA+AGG 0.179265 7:-59445639 MsG0780039190.01.T01:CDS
CTTGCTGTTCTTTATAAATA+TGG 0.182451 7:-59445474 MsG0780039190.01.T01:CDS
ACCACTAAGTTGCCTCAATT+TGG 0.235516 7:+59445083 None:intergenic
CTAAGATAGGTGGTTTCTTT+AGG 0.266794 7:-59445143 MsG0780039190.01.T01:CDS
CAACTTAGTGGTGAGACTTT+TGG 0.269769 7:-59445072 MsG0780039190.01.T01:CDS
GGAGCACAAAGCATGGATTT+TGG 0.279680 7:-59445387 MsG0780039190.01.T01:CDS
AGTCCAACTAACTGGATAAA+AGG 0.306301 7:+59445164 None:intergenic
GATTCTAAGGTCAAATAAAT+TGG 0.310952 7:-59445862 MsG0780039190.01.T01:CDS
AGAATCATCCACTTCTTCTA+AGG 0.328977 7:-59445317 MsG0780039190.01.T01:CDS
ATTTCACCTTCAAGTTCATT+TGG 0.338286 7:-59445765 MsG0780039190.01.T01:CDS
CACTTCCATGCTAGAATTCA+AGG 0.342911 7:-59445834 MsG0780039190.01.T01:CDS
CTTGATCCAAATGAACTTGA+AGG 0.381142 7:+59445759 None:intergenic
TACAAGAGTTCAAGATTCTA+AGG 0.382842 7:-59445875 MsG0780039190.01.T01:CDS
TCAAACCATTCATTGATAAA+GGG 0.385354 7:-59445638 MsG0780039190.01.T01:CDS
AAACATGGTTTCATGAGTTA+AGG 0.389362 7:-59445557 MsG0780039190.01.T01:CDS
CCTATCTTAGTCCAACTAAC+TGG 0.392559 7:+59445156 None:intergenic
TATTTGTAGCATGGAGTGTA+AGG 0.405268 7:+59446087 None:intergenic
AGGTTAACACTTACTCTTGA+TGG 0.407394 7:-59446035 MsG0780039190.01.T01:CDS
GTTTAATCTAGTCCAATGTT+TGG 0.409444 7:+59445362 None:intergenic
TTGAACCCTTTATCAATGAA+TGG 0.409890 7:+59445633 None:intergenic
CCAGTTAGTTGGACTAAGAT+AGG 0.417236 7:-59445156 MsG0780039190.01.T01:CDS
AGAACTTTGGACTCAATTCA+TGG 0.419288 7:-59445672 MsG0780039190.01.T01:CDS
GACAATTCTATCAAGAACTT+TGG 0.425317 7:-59445685 MsG0780039190.01.T01:CDS
AGTGGATTGGTTATTGAAGA+AGG 0.430435 7:-59445024 MsG0780039190.01.T01:CDS
TGAAACCTTCGAGTGGTTTG+AGG 0.456209 7:-59445419 MsG0780039190.01.T01:CDS
AATCTTGGCATGCTTAATCA+AGG 0.462415 7:+59446187 None:intergenic
CATGGAGTGTAAGGGAATCA+TGG 0.469815 7:+59446096 None:intergenic
TTAATGAACCTTAGAAGAAG+TGG 0.474220 7:+59445309 None:intergenic
CAAAGAATTGCATGGTTCAA+AGG 0.491075 7:-59445903 MsG0780039190.01.T01:CDS
TCTAGAGTTGTAGAAAGACT+TGG 0.499455 7:-59445228 MsG0780039190.01.T01:CDS
AGTGGTTTGAGGAATAGTAT+TGG 0.501021 7:-59445408 MsG0780039190.01.T01:CDS
GCCAAATTGAGGCAACTTAG+TGG 0.501027 7:-59445084 MsG0780039190.01.T01:CDS
GAGGTTCTTTATTTGTAGCA+TGG 0.502734 7:+59446078 None:intergenic
CTAGAGTTGTAGAAAGACTT+GGG 0.512263 7:-59445227 MsG0780039190.01.T01:CDS
GAACAAACAAAGTAGTGGAT+TGG 0.525391 7:-59445037 MsG0780039190.01.T01:CDS
ATTTGTAGCATGGAGTGTAA+GGG 0.547978 7:+59446088 None:intergenic
GAAATCCTTGAATTCTAGCA+TGG 0.563462 7:+59445829 None:intergenic
TAGTATTGGAGCACAAAGCA+TGG 0.567516 7:-59445394 MsG0780039190.01.T01:CDS
TGAGCTTGTAACATCACAAG+AGG 0.574114 7:+59446059 None:intergenic
GCTGTTCTTTATAAATATGG+TGG 0.575469 7:-59445471 MsG0780039190.01.T01:CDS
AATGGGTTGAAGCCAAATTG+AGG 0.581220 7:-59445095 MsG0780039190.01.T01:CDS
ATCTTGGCATGCTTAATCAA+GGG 0.583016 7:+59446188 None:intergenic
TTGTGATGTTACAAGCTCAA+AGG 0.584164 7:-59446055 MsG0780039190.01.T01:CDS
TGAAACAAAGGAATCTCACC+AGG 0.588168 7:+59445519 None:intergenic
AGTCACAATTCTTCATGACA+TGG 0.589579 7:-59445788 MsG0780039190.01.T01:CDS
AAGGTACTCTAGCTGAAACA+TGG 0.598518 7:-59445572 MsG0780039190.01.T01:CDS
CTAACTGGATAAAAGGCCAT+AGG 0.598525 7:+59445171 None:intergenic
CTATGGAACAAACAAAGTAG+TGG 0.605613 7:-59445042 MsG0780039190.01.T01:CDS
ACAACTCTAGAAACAAGGTA+AGG 0.607467 7:+59445240 None:intergenic
CTATTCCTCAAACCACTCGA+AGG 0.615322 7:+59445414 None:intergenic
GAAAGACTTGGGAAAAGACA+AGG 0.616882 7:-59445216 MsG0780039190.01.T01:CDS
ACATAGAACAAAGAATTGCA+TGG 0.622172 7:-59445911 MsG0780039190.01.T01:CDS
GTTAGTTGGACTAAGATAGG+TGG 0.632643 7:-59445153 MsG0780039190.01.T01:CDS
TGGTTTCATGAGTTAAGGAG+AGG 0.634296 7:-59445552 MsG0780039190.01.T01:CDS
AGAATTGTCAAGCATGCTTG+AGG 0.640913 7:+59445699 None:intergenic
TATTGAAGAAGGAAGTGTCA+TGG 0.644276 7:-59445013 MsG0780039190.01.T01:CDS
ATACTATTGAAACCTTCGAG+TGG 0.644454 7:-59445426 MsG0780039190.01.T01:CDS
ACTGGATAAAAGGCCATAGG+TGG 0.647063 7:+59445174 None:intergenic
TTTCTACAACTCTAGAAACA+AGG 0.670555 7:+59445235 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAACAAAAATGACAAGTT+TGG + Chr7:59445578-59445597 None:intergenic 20.0%
!!! AACTTGTCATTTTTGTTAAA+AGG - Chr7:59445578-59445597 MsG0780039190.01.T01:CDS 20.0%
!!! TTAGATAGATTTTGAAACAA+AGG + Chr7:59445665-59445684 None:intergenic 20.0%
! AAACTAGAAGTGAAGAAAAA+TGG - Chr7:59446056-59446075 MsG0780039190.01.T01:CDS 25.0%
! AACTAGAAGTGAAGAAAAAT+GGG - Chr7:59446057-59446076 MsG0780039190.01.T01:CDS 25.0%
! ACCTTGTATGAGAAAAAATA+TGG + Chr7:59445039-59445058 None:intergenic 25.0%
! AGAGAAGATAGAAAAAATCT+TGG + Chr7:59445000-59445019 None:intergenic 25.0%
! TCAAACCATTCATTGATAAA+GGG - Chr7:59445531-59445550 MsG0780039190.01.T01:CDS 25.0%
! TGTTCTATGTTGAGATTTAA+TGG + Chr7:59445248-59445267 None:intergenic 25.0%
!! ACTTTTGATGGAAATAAATG+GGG - Chr7:59445896-59445915 MsG0780039190.01.T01:CDS 25.0%
!! GACTTTTGATGGAAATAAAT+GGG - Chr7:59445895-59445914 MsG0780039190.01.T01:CDS 25.0%
!! TGACTTTTGATGGAAATAAA+TGG - Chr7:59445894-59445913 MsG0780039190.01.T01:CDS 25.0%
!! TTCTAATTTCTCTTTTTGCA+TGG + Chr7:59445189-59445208 None:intergenic 25.0%
!!! ACCATATTTTTTCTCATACA+AGG - Chr7:59445035-59445054 MsG0780039190.01.T01:CDS 25.0%
!!! CTTGCTGTTCTTTATAAATA+TGG - Chr7:59445695-59445714 MsG0780039190.01.T01:CDS 25.0%
!!! GATTCTAAGGTCAAATAAAT+TGG - Chr7:59445307-59445326 MsG0780039190.01.T01:CDS 25.0%
!!! TTTTCTTCACTTCTAGTTTT+AGG + Chr7:59446055-59446074 None:intergenic 25.0%
AAACATGGTTTCATGAGTTA+AGG - Chr7:59445612-59445631 MsG0780039190.01.T01:CDS 30.0%
ACATAGAACAAAGAATTGCA+TGG - Chr7:59445258-59445277 MsG0780039190.01.T01:CDS 30.0%
CTCAAACCATTCATTGATAA+AGG - Chr7:59445530-59445549 MsG0780039190.01.T01:CDS 30.0%
GACAATTCTATCAAGAACTT+TGG - Chr7:59445484-59445503 MsG0780039190.01.T01:CDS 30.0%
GCTGTTCTTTATAAATATGG+TGG - Chr7:59445698-59445717 MsG0780039190.01.T01:CDS 30.0%
GTTTAATCTAGTCCAATGTT+TGG + Chr7:59445810-59445829 None:intergenic 30.0%
TACAAGAGTTCAAGATTCTA+AGG - Chr7:59445294-59445313 MsG0780039190.01.T01:CDS 30.0%
TTAATGAACCTTAGAAGAAG+TGG + Chr7:59445863-59445882 None:intergenic 30.0%
TTGAACCCTTTATCAATGAA+TGG + Chr7:59445539-59445558 None:intergenic 30.0%
! ATTTCACCTTCAAGTTCATT+TGG - Chr7:59445404-59445423 MsG0780039190.01.T01:CDS 30.0%
! CTTTTGATGGAAATAAATGG+GGG - Chr7:59445897-59445916 MsG0780039190.01.T01:CDS 30.0%
! TTTCTACAACTCTAGAAACA+AGG + Chr7:59445937-59445956 None:intergenic 30.0%
! TTTTACAATTTTGCCACCTA+TGG - Chr7:59445982-59446001 MsG0780039190.01.T01:CDS 30.0%
!! GAATTTGCTTTGACTTTTGA+TGG - Chr7:59445884-59445903 MsG0780039190.01.T01:CDS 30.0%
!!! GTCTATAGAAAGTGTTTTCA+AGG - Chr7:59445442-59445461 MsG0780039190.01.T01:CDS 30.0%
AATCTTGGCATGCTTAATCA+AGG + Chr7:59444985-59445004 None:intergenic 35.0%
AGAACTTTGGACTCAATTCA+TGG - Chr7:59445497-59445516 MsG0780039190.01.T01:CDS 35.0%
AGAATCATCCACTTCTTCTA+AGG - Chr7:59445852-59445871 MsG0780039190.01.T01:CDS 35.0%
AGGTTAACACTTACTCTTGA+TGG - Chr7:59445134-59445153 MsG0780039190.01.T01:CDS 35.0%
AGTCACAATTCTTCATGACA+TGG - Chr7:59445381-59445400 MsG0780039190.01.T01:CDS 35.0%
AGTCCAACTAACTGGATAAA+AGG + Chr7:59446008-59446027 None:intergenic 35.0%
AGTGGTTTGAGGAATAGTAT+TGG - Chr7:59445761-59445780 MsG0780039190.01.T01:CDS 35.0%
ATACTATTGAAACCTTCGAG+TGG - Chr7:59445743-59445762 MsG0780039190.01.T01:CDS 35.0%
ATCTTGGCATGCTTAATCAA+GGG + Chr7:59444984-59445003 None:intergenic 35.0%
ATTTGTAGCATGGAGTGTAA+GGG + Chr7:59445084-59445103 None:intergenic 35.0%
CAAAGAATTGCATGGTTCAA+AGG - Chr7:59445266-59445285 MsG0780039190.01.T01:CDS 35.0%
CTAAGATAGGTGGTTTCTTT+AGG - Chr7:59446026-59446045 MsG0780039190.01.T01:CDS 35.0%
CTAGAGTTGTAGAAAGACTT+GGG - Chr7:59445942-59445961 MsG0780039190.01.T01:CDS 35.0%
CTATGGAACAAACAAAGTAG+TGG - Chr7:59446127-59446146 MsG0780039190.01.T01:CDS 35.0%
CTTGATCCAAATGAACTTGA+AGG + Chr7:59445413-59445432 None:intergenic 35.0%
GAAATCCTTGAATTCTAGCA+TGG + Chr7:59445343-59445362 None:intergenic 35.0%
TATTTGTAGCATGGAGTGTA+AGG + Chr7:59445085-59445104 None:intergenic 35.0%
TTGTGATGTTACAAGCTCAA+AGG - Chr7:59445114-59445133 MsG0780039190.01.T01:CDS 35.0%
! AACTTAGTGGTGAGACTTTT+GGG - Chr7:59446098-59446117 MsG0780039190.01.T01:CDS 35.0%
! ACAACTCTAGAAACAAGGTA+AGG + Chr7:59445932-59445951 None:intergenic 35.0%
! GAGGTTCTTTATTTGTAGCA+TGG + Chr7:59445094-59445113 None:intergenic 35.0%
! TCTAGAGTTGTAGAAAGACT+TGG - Chr7:59445941-59445960 MsG0780039190.01.T01:CDS 35.0%
! TTTTGCATGGAACGTAGTAA+AGG + Chr7:59445176-59445195 None:intergenic 35.0%
!! AGTGGATTGGTTATTGAAGA+AGG - Chr7:59446145-59446164 MsG0780039190.01.T01:CDS 35.0%
!! GAACAAACAAAGTAGTGGAT+TGG - Chr7:59446132-59446151 MsG0780039190.01.T01:CDS 35.0%
!! TATTGAAGAAGGAAGTGTCA+TGG - Chr7:59446156-59446175 MsG0780039190.01.T01:CDS 35.0%
!! TGGATTTTGGTACCAAACAT+TGG - Chr7:59445795-59445814 MsG0780039190.01.T01:CDS 35.0%
ACCACTAAGTTGCCTCAATT+TGG + Chr7:59446089-59446108 None:intergenic 40.0%
AGAATTGTCAAGCATGCTTG+AGG + Chr7:59445473-59445492 None:intergenic 40.0%
AGGAGAGGAAAAAAAGATCC+TGG - Chr7:59445632-59445651 MsG0780039190.01.T01:CDS 40.0%
CACTTCCATGCTAGAATTCA+AGG - Chr7:59445335-59445354 MsG0780039190.01.T01:CDS 40.0%
CCAGTTAGTTGGACTAAGAT+AGG - Chr7:59446013-59446032 MsG0780039190.01.T01:CDS 40.0%
CCTATCTTAGTCCAACTAAC+TGG + Chr7:59446016-59446035 None:intergenic 40.0%
CTAACTGGATAAAAGGCCAT+AGG + Chr7:59446001-59446020 None:intergenic 40.0%
GAAAGACTTGGGAAAAGACA+AGG - Chr7:59445953-59445972 MsG0780039190.01.T01:CDS 40.0%
GTTAGTTGGACTAAGATAGG+TGG - Chr7:59446016-59446035 MsG0780039190.01.T01:CDS 40.0%
TGAAACAAAGGAATCTCACC+AGG + Chr7:59445653-59445672 None:intergenic 40.0%
TGAGCTTGTAACATCACAAG+AGG + Chr7:59445113-59445132 None:intergenic 40.0%
TGGTTTCATGAGTTAAGGAG+AGG - Chr7:59445617-59445636 MsG0780039190.01.T01:CDS 40.0%
! AAGGTACTCTAGCTGAAACA+TGG - Chr7:59445597-59445616 MsG0780039190.01.T01:CDS 40.0%
! ACTTAGTGGTGAGACTTTTG+GGG - Chr7:59446099-59446118 MsG0780039190.01.T01:CDS 40.0%
! CAACTTAGTGGTGAGACTTT+TGG - Chr7:59446097-59446116 MsG0780039190.01.T01:CDS 40.0%
! GGAAATAAATGGGGGCATAA+TGG - Chr7:59445905-59445924 MsG0780039190.01.T01:CDS 40.0%
! TAGTATTGGAGCACAAAGCA+TGG - Chr7:59445775-59445794 MsG0780039190.01.T01:CDS 40.0%
! TGGCCTTTTATCCAGTTAGT+TGG - Chr7:59446002-59446021 MsG0780039190.01.T01:CDS 40.0%
!! AATGGGTTGAAGCCAAATTG+AGG - Chr7:59446074-59446093 MsG0780039190.01.T01:CDS 40.0%
!!! AGACTTTTGGGGTTCATCTA+TGG - Chr7:59446110-59446129 MsG0780039190.01.T01:CDS 40.0%
ACTGGATAAAAGGCCATAGG+TGG + Chr7:59445998-59446017 None:intergenic 45.0%
CATGGAGTGTAAGGGAATCA+TGG + Chr7:59445076-59445095 None:intergenic 45.0%
CTATTCCTCAAACCACTCGA+AGG + Chr7:59445758-59445777 None:intergenic 45.0%
GCCAAATTGAGGCAACTTAG+TGG - Chr7:59446085-59446104 MsG0780039190.01.T01:CDS 45.0%
TGAAACCTTCGAGTGGTTTG+AGG - Chr7:59445750-59445769 MsG0780039190.01.T01:CDS 45.0%
! GGAGCACAAAGCATGGATTT+TGG - Chr7:59445782-59445801 MsG0780039190.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 59444972 59446219 59444972 ID=MsG0780039190.01;Name=MsG0780039190.01
Chr7 mRNA 59444972 59446219 59444972 ID=MsG0780039190.01.T01;Parent=MsG0780039190.01;Name=MsG0780039190.01.T01;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|415
Chr7 exon 59444972 59446219 59444972 ID=MsG0780039190.01.T01:exon:7030;Parent=MsG0780039190.01.T01
Chr7 CDS 59444972 59446219 59444972 ID=MsG0780039190.01.T01:cds;Parent=MsG0780039190.01.T01
Gene Sequence

>MsG0780039190.01.T01

ATGTTTAATCCCTTGATTAAGCATGCCAAGATTTTTTCTATCTTCTCTCTAATCACATTATCTACCATATTTTTTCTCATACAAGGTGATCATGTTATCTACCATGATTCCCTTACACTCCATGCTACAAATAAAGAACCTCTTGTGATGTTACAAGCTCAAAGGTTAACACTTACTCTTGATGGTCACAATTACAATAAACCTTTACTACGTTCCATGCAAAAAGAGAAATTAGAAGAAGTTGATTATGCAAATCAAAAAGTACTAGTTGCTCCATTAAATCTCAACATAGAACAAAGAATTGCATGGTTCAAAGGTAATCTACAAGAGTTCAAGATTCTAAGGTCAAATAAATTGGCAAAGCACTTCCATGCTAGAATTCAAGGATTTCACAAAAACAATTTATGTGAGTCACAATTCTTCATGACATGGATTTCACCTTCAAGTTCATTTGGATCAAGAGAAACTTTGTCTATAGAAAGTGTTTTCAAGGTTCAACCTCAAGCATGCTTGACAATTCTATCAAGAACTTTGGACTCAATTCATGGCTACAAAATTCTCAAACCATTCATTGATAAAGGGTTCAAAGTTCAAGCTATAACACCAAACTTGTCATTTTTGTTAAAAGGTACTCTAGCTGAAACATGGTTTCATGAGTTAAGGAGAGGAAAAAAAGATCCTGGTGAGATTCCTTTGTTTCAAAATCTATCTAACTTGATAAGACTTGCTGTTCTTTATAAATATGGTGGTGTTTATATAGACATAGACTTTATACTATTGAAACCTTCGAGTGGTTTGAGGAATAGTATTGGAGCACAAAGCATGGATTTTGGTACCAAACATTGGACTAGATTAAACAATGCTGTTCTTATTTTTGATAAGAATCATCCACTTCTTCTAAGGTTCATTAATGAATTTGCTTTGACTTTTGATGGAAATAAATGGGGGCATAATGGTCCTTACCTTGTTTCTAGAGTTGTAGAAAGACTTGGGAAAAGACAAGGTTTCAATTTTACAATTTTGCCACCTATGGCCTTTTATCCAGTTAGTTGGACTAAGATAGGTGGTTTCTTTAGGAAGCCTAAAACTAGAAGTGAAGAAAAATGGGTTGAAGCCAAATTGAGGCAACTTAGTGGTGAGACTTTTGGGGTTCATCTATGGAACAAACAAAGTAGTGGATTGGTTATTGAAGAAGGAAGTGTCATGGCAAGACTTGTATCAAATCATTGTATCATTTGTAAGTTGTAA

Protein sequence

>MsG0780039190.01.T01

MFNPLIKHAKIFSIFSLITLSTIFFLIQGDHVIYHDSLTLHATNKEPLVMLQAQRLTLTLDGHNYNKPLLRSMQKEKLEEVDYANQKVLVAPLNLNIEQRIAWFKGNLQEFKILRSNKLAKHFHARIQGFHKNNLCESQFFMTWISPSSSFGSRETLSIESVFKVQPQACLTILSRTLDSIHGYKILKPFIDKGFKVQAITPNLSFLLKGTLAETWFHELRRGKKDPGEIPLFQNLSNLIRLAVLYKYGGVYIDIDFILLKPSSGLRNSIGAQSMDFGTKHWTRLNNAVLIFDKNHPLLLRFINEFALTFDGNKWGHNGPYLVSRVVERLGKRQGFNFTILPPMAFYPVSWTKIGGFFRKPKTRSEEKWVEAKLRQLSGETFGVHLWNKQSSGLVIEEGSVMARLVSNHCIICKL*