AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006853.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006853.01.T01 MTR_2g015540 98.750 80 1 0 1 80 1 80 8.24e-50 151
MsG0280006853.01.T01 MTR_4g079680 75.000 76 19 0 5 80 2 77 7.79e-35 113
MsG0280006853.01.T01 MTR_2g090695 59.459 74 30 0 7 80 5 78 1.24e-20 77.8
MsG0280006853.01.T01 MTR_4g078840 56.944 72 31 0 9 80 8 79 2.12e-14 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006853.01.T01 AT2G31090 75.000 76 17 1 5 80 2 75 3.46e-27 94.4
MsG0280006853.01.T01 AT2G20562 60.870 69 27 0 12 80 5 73 5.38e-20 76.3

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTAGGTGCAGTTATT+TGG 0.227999 2:+7104274 MsG0280006853.01.T01:CDS
AACCACTCCATAACCTTAAT+TGG 0.239487 2:-7104498 None:intergenic
TCTCTCATAATCTCCTTTGT+AGG 0.287883 2:+7104261 MsG0280006853.01.T01:CDS
TCTCATCAAGGCTCCAATTA+AGG 0.314893 2:+7104485 MsG0280006853.01.T01:CDS
CAAGGCTCCAATTAAGGTTA+TGG 0.360751 2:+7104491 MsG0280006853.01.T01:CDS
TCTATTCTAACATGGAATCT+TGG 0.376692 2:-7104526 None:intergenic
GGTGCAGTTATTTGGATTGT+TGG 0.388294 2:+7104282 MsG0280006853.01.T01:CDS
GTGCAGTTATTTGGATTGTT+GGG 0.397665 2:+7104283 MsG0280006853.01.T01:CDS
GTTTGCTCTGGCTCTCATCA+AGG 0.420977 2:+7104473 MsG0280006853.01.T01:CDS
CCTCATAGTTGAGTTTGCTC+TGG 0.438328 2:+7104461 MsG0280006853.01.T01:CDS
GATTGTAAATTCAGGCCTCT+TGG 0.461509 2:+7104207 MsG0280006853.01.T01:CDS
ATGAGAGACAAGAAAGCAAA+AGG 0.478563 2:-7104246 None:intergenic
AGAAAGCAAAAGGAAGGCCT+AGG 0.499755 2:-7104236 None:intergenic
TTAACCATGTGTTGCTGTTG+TGG 0.511293 2:+7104180 None:intergenic
CCAGAGCAAACTCAACTATG+AGG 0.515083 2:-7104461 None:intergenic
GTGGTGAGGATTGTAAATTC+AGG 0.531054 2:+7104199 MsG0280006853.01.T01:CDS
CCAAATAACTGCACCTACAA+AGG 0.535186 2:-7104274 None:intergenic
ATATCTCTTCTATTCTAACA+TGG 0.596081 2:-7104534 None:intergenic
CATGTGTTGCTGTTGTGGTG+AGG 0.609312 2:+7104185 None:intergenic
GAGACAAGAAAGCAAAAGGA+AGG 0.614663 2:-7104242 None:intergenic
AGGGTCACGCAAAGACAGCA+TGG 0.623057 2:-7104441 None:intergenic
CAGAGCAAACTCAACTATGA+GGG 0.623831 2:-7104460 None:intergenic
CTCACCACAACAGCAACACA+TGG 0.630485 2:-7104184 None:intergenic
CTCCAATTAAGGTTATGGAG+TGG 0.661349 2:+7104496 MsG0280006853.01.T01:CDS
AGGCCTAGGAGAAAACCAAG+AGG 0.747286 2:-7104222 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAAATACTAATAGAT+GGG - Chr2:7104325-7104344 None:intergenic 15.0%
!! CAAAAGAAAATACTAATAGA+TGG - Chr2:7104326-7104345 None:intergenic 20.0%
! AATGTGTATGATGCTATATA+TGG + Chr2:7104393-7104412 MsG0280006853.01.T01:intron 25.0%
AACCACTCCATAACCTTAAT+TGG - Chr2:7104501-7104520 None:intergenic 35.0%
ACTAATAGATGGGAAAAGAG+AGG - Chr2:7104315-7104334 None:intergenic 35.0%
ATGAGAGACAAGAAAGCAAA+AGG - Chr2:7104249-7104268 None:intergenic 35.0%
CTAATAGATGGGAAAAGAGA+GGG - Chr2:7104314-7104333 None:intergenic 35.0%
GTGCAGTTATTTGGATTGTT+GGG + Chr2:7104283-7104302 MsG0280006853.01.T01:CDS 35.0%
TCTCTCATAATCTCCTTTGT+AGG + Chr2:7104261-7104280 MsG0280006853.01.T01:CDS 35.0%
TGTATGATGCTATATATGGC+AGG + Chr2:7104397-7104416 MsG0280006853.01.T01:intron 35.0%
CAAGGCTCCAATTAAGGTTA+TGG + Chr2:7104491-7104510 MsG0280006853.01.T01:CDS 40.0%
CAGAGCAAACTCAACTATGA+GGG - Chr2:7104463-7104482 None:intergenic 40.0%
CCAAATAACTGCACCTACAA+AGG - Chr2:7104277-7104296 None:intergenic 40.0%
CCTTTGTAGGTGCAGTTATT+TGG + Chr2:7104274-7104293 MsG0280006853.01.T01:CDS 40.0%
CTCCAATTAAGGTTATGGAG+TGG + Chr2:7104496-7104515 MsG0280006853.01.T01:CDS 40.0%
GAGACAAGAAAGCAAAAGGA+AGG - Chr2:7104245-7104264 None:intergenic 40.0%
GATTGTAAATTCAGGCCTCT+TGG + Chr2:7104207-7104226 MsG0280006853.01.T01:CDS 40.0%
GGTGCAGTTATTTGGATTGT+TGG + Chr2:7104282-7104301 MsG0280006853.01.T01:CDS 40.0%
GTGGTGAGGATTGTAAATTC+AGG + Chr2:7104199-7104218 MsG0280006853.01.T01:CDS 40.0%
TCTCATCAAGGCTCCAATTA+AGG + Chr2:7104485-7104504 MsG0280006853.01.T01:CDS 40.0%
AGAAAGCAAAAGGAAGGCCT+AGG - Chr2:7104239-7104258 None:intergenic 45.0%
CCAGAGCAAACTCAACTATG+AGG - Chr2:7104464-7104483 None:intergenic 45.0%
! CCTCATAGTTGAGTTTGCTC+TGG + Chr2:7104461-7104480 MsG0280006853.01.T01:CDS 45.0%
AGGCCTAGGAGAAAACCAAG+AGG - Chr2:7104225-7104244 None:intergenic 50.0%
!! AGGCCTCTTGGTTTTCTCCT+AGG + Chr2:7104219-7104238 MsG0280006853.01.T01:CDS 50.0%
!! GTTTGCTCTGGCTCTCATCA+AGG + Chr2:7104473-7104492 MsG0280006853.01.T01:CDS 50.0%
AGGGTCACGCAAAGACAGCA+TGG - Chr2:7104444-7104463 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 7104186 7104542 7104186 ID=MsG0280006853.01;Name=MsG0280006853.01
Chr2 mRNA 7104186 7104542 7104186 ID=MsG0280006853.01.T01;Parent=MsG0280006853.01;Name=MsG0280006853.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|0|0|2|0|80
Chr2 exon 7104186 7104304 7104186 ID=MsG0280006853.01.T01:exon:2126;Parent=MsG0280006853.01.T01
Chr2 exon 7104419 7104542 7104419 ID=MsG0280006853.01.T01:exon:2127;Parent=MsG0280006853.01.T01
Chr2 CDS 7104186 7104304 7104186 ID=MsG0280006853.01.T01:cds;Parent=MsG0280006853.01.T01
Chr2 CDS 7104419 7104542 7104419 ID=MsG0280006853.01.T01:cds;Parent=MsG0280006853.01.T01
Gene Sequence

>MsG0280006853.01.T01

ATGTGTTGCTGTTGTGGTGAGGATTGTAAATTCAGGCCTCTTGGTTTTCTCCTAGGCCTTCCTTTTGCTTTCTTGTCTCTCATAATCTCCTTTGTAGGTGCAGTTATTTGGATTGTTGGGTTGACTTTGACATGTATATGTCCATGCTGTCTTTGCGTGACCCTCATAGTTGAGTTTGCTCTGGCTCTCATCAAGGCTCCAATTAAGGTTATGGAGTGGTTCACATCCAAGATTCCATGTTAG

Protein sequence

>MsG0280006853.01.T01

MCCCCGEDCKFRPLGFLLGLPFAFLSLIISFVGAVIWIVGLTLTCICPCCLCVTLIVEFALALIKAPIKVMEWFTSKIPC*