AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000138.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000138.01.T01 MTR_1g022395 72.121 165 12 2 1 163 1 133 4.87e-78 228
MsG0180000138.01.T01 MTR_3g111970 51.205 166 40 5 1 163 1 128 5.22e-47 150
MsG0180000138.01.T01 MTR_3g111970 49.673 153 36 5 1 150 1 115 1.21e-38 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000138.01.T01 AT1G19530 32.716 162 58 4 1 161 1 112 2.74e-13 63.2
MsG0180000138.01.T01 AT1G19530 32.716 162 58 4 1 161 1 112 2.74e-13 63.2

Find 51 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATGAAATTGATGCTTAT+TGG 0.156482 1:-1945032 MsG0180000138.01.T01:CDS
AATCATATGGTACCTGATTT+AGG 0.175839 1:+1945210 None:intergenic
CTGAATAGTCTTTGACAAAT+TGG 0.206876 1:+1944966 None:intergenic
GAACGTACTTGACACAAATT+TGG 0.215908 1:-1943920 MsG0180000138.01.T01:CDS
GTCAAGTACGTTCATATTAA+AGG 0.224800 1:+1943930 None:intergenic
CTGTTTCAGCCATACTTAAA+AGG 0.239435 1:-1945071 MsG0180000138.01.T01:intron
AGACTAAGAGTACTACAAAT+TGG 0.249038 1:-1945348 MsG0180000138.01.T01:five_prime_UTR
GCAAATCAACGTGTGTTTCT+TGG 0.281985 1:-1943442 MsG0180000138.01.T01:three_prime_UTR
CAATTTGTCAAAGACTATTC+AGG 0.285232 1:-1944965 MsG0180000138.01.T01:CDS
ACCACAATGCAATTTCTAAA+TGG 0.294299 1:+1945307 None:intergenic
CAGTTTCACGTTGCCAGTTT+GGG 0.309436 1:-1943651 MsG0180000138.01.T01:CDS
CTAAACATTAATTTCAAACT+TGG 0.322769 1:+1944027 None:intergenic
CAAGTTTGAAATTAATGTTT+AGG 0.334583 1:-1944026 MsG0180000138.01.T01:CDS
GCAGGTGGACTAAGAGTAAC+TGG 0.334701 1:-1943728 MsG0180000138.01.T01:intron
GCATGGGCAGATCCAACTTA+TGG 0.351906 1:-1944911 MsG0180000138.01.T01:intron
TCAAGTACGTTCATATTAAA+GGG 0.359264 1:+1943931 None:intergenic
ACAGTTTCACGTTGCCAGTT+TGG 0.359415 1:-1943652 MsG0180000138.01.T01:CDS
TCTTCAATTGATCCTAAATC+AGG 0.362673 1:-1945222 MsG0180000138.01.T01:intron
TTGGATGCAGCTTCCATCAT+TGG 0.363993 1:+1943687 None:intergenic
TCAATTTGTCGATCCCAAAC+TGG 0.421196 1:+1943638 None:intergenic
GGGTTCTCTTATGGCAGGAT+GGG 0.445491 1:-1945250 MsG0180000138.01.T01:CDS
TACTTGCCACTTTCCAGCAT+GGG 0.450466 1:-1944927 MsG0180000138.01.T01:CDS
GACTAAGAGTACTACAAATT+GGG 0.460520 1:-1945347 MsG0180000138.01.T01:five_prime_UTR
TCTGAATGAGCCACCAATGA+TGG 0.467236 1:-1943700 MsG0180000138.01.T01:CDS
CCATGCCCGTGGCCCACGTA+AGG 0.471373 1:-1943957 MsG0180000138.01.T01:CDS
GGGTCCCTTACGTGGGCCAC+GGG 0.472849 1:+1943952 None:intergenic
GGGGTCCCTTACGTGGGCCA+CGG 0.474803 1:+1943951 None:intergenic
CCTTACGTGGGCCACGGGCA+TGG 0.492032 1:+1943957 None:intergenic
GGTTCTCTTATGGCAGGATG+GGG 0.492223 1:-1945249 MsG0180000138.01.T01:CDS
CAATCACTAACTTCATCTAC+GGG 0.495529 1:+1943613 None:intergenic
GAAATCAAAGAAGAAGATAG+AGG 0.499005 1:-1945010 MsG0180000138.01.T01:CDS
TAAGTTGGATCTGCCCATGC+TGG 0.504399 1:+1944914 None:intergenic
GATGCAGCTTCCATCATTGG+TGG 0.505474 1:+1943690 None:intergenic
TGGGTTCTCTTATGGCAGGA+TGG 0.507870 1:-1945251 MsG0180000138.01.T01:CDS
ACAAATTGGAAATTGCTGTA+AGG 0.512610 1:+1944980 None:intergenic
TCAATCACTAACTTCATCTA+CGG 0.513170 1:+1943612 None:intergenic
AAACTGTGCTGCATACTTGT+TGG 0.515262 1:+1943668 None:intergenic
TATTAAAGGGGTCCCTTACG+TGG 0.530982 1:+1943944 None:intergenic
AAAATGGGTTCTCTTATGGC+AGG 0.548408 1:-1945255 MsG0180000138.01.T01:CDS
CATGCCCGTGGCCCACGTAA+GGG 0.568293 1:-1943956 MsG0180000138.01.T01:CDS
GGTAGCCGCAAACCATAAGT+TGG 0.578815 1:+1944899 None:intergenic
CTACTTGCCACTTTCCAGCA+TGG 0.590312 1:-1944928 MsG0180000138.01.T01:CDS
TGCTGGAAAGTGGCAAGTAG+AGG 0.605008 1:+1944931 None:intergenic
TCCATTTAGAAATTGCATTG+TGG 0.619587 1:-1945308 MsG0180000138.01.T01:five_prime_UTR
GATCTGCCCATGCTGGAAAG+TGG 0.635937 1:+1944921 None:intergenic
GACATAAATATATACATACA+AGG 0.637376 1:+1943563 None:intergenic
GAAGTCAATGACCATGCCCG+TGG 0.649977 1:-1943968 MsG0180000138.01.T01:CDS
CAAGTACGTTCATATTAAAG+GGG 0.651488 1:+1943932 None:intergenic
ATTAAAGGGGTCCCTTACGT+GGG 0.678500 1:+1943945 None:intergenic
CAGGTGGACTAAGAGTAACT+GGG 0.688506 1:-1943727 MsG0180000138.01.T01:CDS
AATCACTAACTTCATCTACG+GGG 0.796732 1:+1943614 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAAATCATATATATATTT+CGG + Chr1:1944522-1944541 None:intergenic 10.0%
!! TGAAATAAAAACATTAAAAT+TGG + Chr1:1944660-1944679 None:intergenic 10.0%
!!! ATTTATTTTTTTGGAAAATT+TGG + Chr1:1943860-1943879 None:intergenic 10.0%
!! AATAAATAAAATACATACGA+AGG - Chr1:1943873-1943892 MsG0180000138.01.T01:intron 15.0%
!! TATAGTATTATATATAGTGT+TGG + Chr1:1944213-1944232 None:intergenic 15.0%
!!! AAATACATCTTTAATTTCAT+TGG - Chr1:1944028-1944047 MsG0180000138.01.T01:CDS 15.0%
!!! TTAATGTTTTTATTTCATGA+TGG - Chr1:1944663-1944682 MsG0180000138.01.T01:intron 15.0%
!!! TTTTACACACAATATTAAAA+TGG + Chr1:1944631-1944650 None:intergenic 15.0%
!! AATCATTATTAGAACTTAGA+CGG - Chr1:1943604-1943623 MsG0180000138.01.T01:three_prime_UTR 20.0%
!! CATATGCATAATTCATATAT+AGG + Chr1:1944954-1944973 None:intergenic 20.0%
!! CTAAACATTAATTTCAAACT+TGG + Chr1:1944703-1944722 None:intergenic 20.0%
!! CTATATATGAATTATGCATA+TGG - Chr1:1944952-1944971 MsG0180000138.01.T01:CDS 20.0%
!! GACATAAATATATACATACA+AGG + Chr1:1945167-1945186 None:intergenic 20.0%
!! TGATTCTATACAGAAAAAAT+GGG - Chr1:1943457-1943476 MsG0180000138.01.T01:three_prime_UTR 20.0%
!! TTGATTCTATACAGAAAAAA+TGG - Chr1:1943456-1943475 MsG0180000138.01.T01:three_prime_UTR 20.0%
!!! AAGTATCGTTATTAATCATA+TGG + Chr1:1943533-1943552 None:intergenic 20.0%
!!! CAAGTTTGAAATTAATGTTT+AGG - Chr1:1944701-1944720 MsG0180000138.01.T01:intron 20.0%
!!! CTACTATTTTCTTTTTCTTT+TGG + Chr1:1944181-1944200 None:intergenic 20.0%
!!! TATTAATTTAATTTGAGTGC+AGG - Chr1:1944981-1945000 MsG0180000138.01.T01:CDS 20.0%
! AAGATGAAATTGATGCTTAT+TGG - Chr1:1943695-1943714 MsG0180000138.01.T01:CDS 25.0%
! ACACTTGAAAAAATCAACAA+GGG - Chr1:1944149-1944168 MsG0180000138.01.T01:intron 25.0%
! ACTATAATAATTGGAAGAGT+AGG + Chr1:1944867-1944886 None:intergenic 25.0%
! ATATATTTCGGATTTCACTT+AGG + Chr1:1944510-1944529 None:intergenic 25.0%
! GAACCCTAATAATTGTTAAT+TGG + Chr1:1943946-1943965 None:intergenic 25.0%
! GCTCCAATTAACAATTATTA+GGG - Chr1:1943940-1943959 MsG0180000138.01.T01:CDS 25.0%
! TACACTTGAAAAAATCAACA+AGG - Chr1:1944148-1944167 MsG0180000138.01.T01:intron 25.0%
! TCAAGTACGTTCATATTAAA+GGG + Chr1:1944799-1944818 None:intergenic 25.0%
! TGCTCCAATTAACAATTATT+AGG - Chr1:1943939-1943958 MsG0180000138.01.T01:CDS 25.0%
!! AGTATTATATATAGTGTTGG+TGG + Chr1:1944210-1944229 None:intergenic 25.0%
!! TAGTTTTCTAATATGCAAGA+AGG + Chr1:1944463-1944482 None:intergenic 25.0%
!!! GTATTATATATAGTGTTGGT+GGG + Chr1:1944209-1944228 None:intergenic 25.0%
!!! TATTATATATAGTGTTGGTG+GGG + Chr1:1944208-1944227 None:intergenic 25.0%
!!! TTTTTTTGGAAAATTTGGTG+TGG + Chr1:1943855-1943874 None:intergenic 25.0%
AACTTATCAACAAAAATGGC+TGG - Chr1:1944833-1944852 MsG0180000138.01.T01:intron 30.0%
AATATGCAAGAAGGACTAAA+AGG + Chr1:1944454-1944473 None:intergenic 30.0%
AATCATATGGTACCTGATTT+AGG + Chr1:1943520-1943539 None:intergenic 30.0%
AATGAAATGTCAGAGAAAGT+TGG - Chr1:1943906-1943925 MsG0180000138.01.T01:CDS 30.0%
ACAAATTGGAAATTGCTGTA+AGG + Chr1:1943750-1943769 None:intergenic 30.0%
ACCACAATGCAATTTCTAAA+TGG + Chr1:1943423-1943442 None:intergenic 30.0%
ACGCGACAAACATTAATTAT+TGG - Chr1:1944365-1944384 MsG0180000138.01.T01:intron 30.0%
ACTCAAACACACAAGAAAAA+TGG + Chr1:1944086-1944105 None:intergenic 30.0%
AGACGGTACTTATATTTATG+AGG - Chr1:1943621-1943640 MsG0180000138.01.T01:exon 30.0%
CAAGTACGTTCATATTAAAG+GGG + Chr1:1944798-1944817 None:intergenic 30.0%
CAATTTGTCAAAGACTATTC+AGG - Chr1:1943762-1943781 MsG0180000138.01.T01:intron 30.0%
CTGAATAGTCTTTGACAAAT+TGG + Chr1:1943764-1943783 None:intergenic 30.0%
GAAATCAAAGAAGAAGATAG+AGG - Chr1:1943717-1943736 MsG0180000138.01.T01:CDS 30.0%
GACGGTACTTATATTTATGA+GGG - Chr1:1943622-1943641 MsG0180000138.01.T01:exon 30.0%
GAGCAACTTATCAACAAAAA+TGG - Chr1:1944829-1944848 MsG0180000138.01.T01:intron 30.0%
GTCAAGTACGTTCATATTAA+AGG + Chr1:1944800-1944819 None:intergenic 30.0%
TCAATCACTAACTTCATCTA+CGG + Chr1:1945118-1945137 None:intergenic 30.0%
TCCATTTAGAAATTGCATTG+TGG - Chr1:1943419-1943438 MsG0180000138.01.T01:three_prime_UTR 30.0%
TCTTCAATTGATCCTAAATC+AGG - Chr1:1943505-1943524 MsG0180000138.01.T01:three_prime_UTR 30.0%
TGTGCTTAGTATTTATGTTG+CGG - Chr1:1943574-1943593 MsG0180000138.01.T01:three_prime_UTR 30.0%
TTACTCTAGTAGAAGAATAC+TGG + Chr1:1944334-1944353 None:intergenic 30.0%
TTTCTTGTGTGTTTGAGTAA+TGG - Chr1:1944088-1944107 MsG0180000138.01.T01:intron 30.0%
! AGAAAAAATGGGTTCTCTTA+TGG - Chr1:1943468-1943487 MsG0180000138.01.T01:three_prime_UTR 30.0%
! TTTCATCTTTTGTAAGTGAC+TGG + Chr1:1943684-1943703 None:intergenic 30.0%
!! AGACTAAGAGTACTACAAAT+TGG - Chr1:1943379-1943398 MsG0180000138.01.T01:three_prime_UTR 30.0%
!! GACTAAGAGTACTACAAATT+GGG - Chr1:1943380-1943399 MsG0180000138.01.T01:three_prime_UTR 30.0%
!! TAATTTAATTTGAGTGCAGG+TGG - Chr1:1944984-1945003 MsG0180000138.01.T01:CDS 30.0%
AATCACTAACTTCATCTACG+GGG + Chr1:1945116-1945135 None:intergenic 35.0%
CAATCACTAACTTCATCTAC+GGG + Chr1:1945117-1945136 None:intergenic 35.0%
CTGTTTCAGCCATACTTAAA+AGG - Chr1:1943656-1943675 MsG0180000138.01.T01:CDS 35.0%
GAACGTACTTGACACAAATT+TGG - Chr1:1944807-1944826 MsG0180000138.01.T01:intron 35.0%
TATTTCGGATTTCACTTAGG+TGG + Chr1:1944507-1944526 None:intergenic 35.0%
!! TGAAACTTCTTTTCAGGATC+AGG + Chr1:1944739-1944758 None:intergenic 35.0%
!! TGACTAAGAGCACTACAATT+TGG - Chr1:1944236-1944255 MsG0180000138.01.T01:intron 35.0%
!! TGACTGGTTCCTTTTAAGTA+TGG + Chr1:1943668-1943687 None:intergenic 35.0%
!!! GACTTCTGAAACTTCTTTTC+AGG + Chr1:1944745-1944764 None:intergenic 35.0%
!!! TTTTGGAAAATTTGGTGTGG+CGG + Chr1:1943852-1943871 None:intergenic 35.0%
AAAAGAAGAAGCAGACAGAG+TGG + Chr1:1944062-1944081 None:intergenic 40.0%
AAACTGTGCTGCATACTTGT+TGG + Chr1:1945062-1945081 None:intergenic 40.0%
ATGTTTGTCGCGTCTCAAAT+AGG + Chr1:1944358-1944377 None:intergenic 40.0%
CAGATCCAACTTATGGTTTG+CGG - Chr1:1943823-1943842 MsG0180000138.01.T01:intron 40.0%
CCAAAAACGAACAACGCTAT+TGG + Chr1:1945223-1945242 None:intergenic 40.0%
GCAAATCAACGTGTGTTTCT+TGG - Chr1:1945285-1945304 MsG0180000138.01.T01:five_prime_UTR 40.0%
TCAATTTGTCGATCCCAAAC+TGG + Chr1:1945092-1945111 None:intergenic 40.0%
! AAAATGGGTTCTCTTATGGC+AGG - Chr1:1943472-1943491 MsG0180000138.01.T01:three_prime_UTR 40.0%
! CCAATAGCGTTGTTCGTTTT+TGG - Chr1:1945220-1945239 MsG0180000138.01.T01:intron 40.0%
ACAGTTTCACGTTGCCAGTT+TGG - Chr1:1945075-1945094 MsG0180000138.01.T01:intron 45.0%
ATTAAAGGGGTCCCTTACGT+GGG + Chr1:1944785-1944804 None:intergenic 45.0%
CAGTTTCACGTTGCCAGTTT+GGG - Chr1:1945076-1945095 MsG0180000138.01.T01:intron 45.0%
TATTAAAGGGGTCCCTTACG+TGG + Chr1:1944786-1944805 None:intergenic 45.0%
TCTGAATGAGCCACCAATGA+TGG - Chr1:1945027-1945046 MsG0180000138.01.T01:CDS 45.0%
TTGGATGCAGCTTCCATCAT+TGG + Chr1:1945043-1945062 None:intergenic 45.0%
!! CAGGTGGACTAAGAGTAACT+GGG - Chr1:1945000-1945019 MsG0180000138.01.T01:CDS 45.0%
!! TACTTGCCACTTTCCAGCAT+GGG - Chr1:1943800-1943819 MsG0180000138.01.T01:intron 45.0%
!! TTATATATAACTATAATAAT+TGG + Chr1:1944876-1944895 None:intergenic 5.0%
!! TTATTATAGTTATATATAAT+TGG - Chr1:1944877-1944896 MsG0180000138.01.T01:intron 5.0%
!!! ATGTATTTTATTTATTTTTT+TGG + Chr1:1943869-1943888 None:intergenic 5.0%
AAGAAGCAGACAGAGTGGTG+GGG + Chr1:1944057-1944076 None:intergenic 50.0%
AGAAGAAGCAGACAGAGTGG+TGG + Chr1:1944059-1944078 None:intergenic 50.0%
GAAGAAGCAGACAGAGTGGT+GGG + Chr1:1944058-1944077 None:intergenic 50.0%
GATGCAGCTTCCATCATTGG+TGG + Chr1:1945040-1945059 None:intergenic 50.0%
GCATGGGCAGATCCAACTTA+TGG - Chr1:1943816-1943835 MsG0180000138.01.T01:intron 50.0%
GGGTTCTCTTATGGCAGGAT+GGG - Chr1:1943477-1943496 MsG0180000138.01.T01:three_prime_UTR 50.0%
GGTAGCCGCAAACCATAAGT+TGG + Chr1:1943831-1943850 None:intergenic 50.0%
GGTTCTCTTATGGCAGGATG+GGG - Chr1:1943478-1943497 MsG0180000138.01.T01:three_prime_UTR 50.0%
TGCTGGAAAGTGGCAAGTAG+AGG + Chr1:1943799-1943818 None:intergenic 50.0%
TGGGTTCTCTTATGGCAGGA+TGG - Chr1:1943476-1943495 MsG0180000138.01.T01:three_prime_UTR 50.0%
! TAAGTTGGATCTGCCCATGC+TGG + Chr1:1943816-1943835 None:intergenic 50.0%
!! CTACTTGCCACTTTCCAGCA+TGG - Chr1:1943799-1943818 MsG0180000138.01.T01:intron 50.0%
!! GCAGGTGGACTAAGAGTAAC+TGG - Chr1:1944999-1945018 MsG0180000138.01.T01:CDS 50.0%
GAAGTCAATGACCATGCCCG+TGG - Chr1:1944759-1944778 MsG0180000138.01.T01:intron 55.0%
GATCTGCCCATGCTGGAAAG+TGG + Chr1:1943809-1943828 None:intergenic 55.0%
CATGCCCGTGGCCCACGTAA+GGG - Chr1:1944771-1944790 MsG0180000138.01.T01:intron 65.0%
CCATGCCCGTGGCCCACGTA+AGG - Chr1:1944770-1944789 MsG0180000138.01.T01:intron 70.0%
CCTTACGTGGGCCACGGGCA+TGG + Chr1:1944773-1944792 None:intergenic 70.0%
GGGGTCCCTTACGTGGGCCA+CGG + Chr1:1944779-1944798 None:intergenic 70.0%
GGGTCCCTTACGTGGGCCAC+GGG + Chr1:1944778-1944797 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 1943375 1945374 1943375 ID=MsG0180000138.01;Name=MsG0180000138.01
Chr1 mRNA 1943375 1945374 1943375 ID=MsG0180000138.01.T01;Parent=MsG0180000138.01;Name=MsG0180000138.01.T01;_AED=0.45;_eAED=0.48;_QI=100|0.66|0.5|1|1|1|4|263|164
Chr1 exon 1945223 1945374 1945223 ID=MsG0180000138.01.T01:exon:6886;Parent=MsG0180000138.01.T01
Chr1 exon 1944912 1945084 1944912 ID=MsG0180000138.01.T01:exon:6887;Parent=MsG0180000138.01.T01
Chr1 exon 1943895 1944055 1943895 ID=MsG0180000138.01.T01:exon:6888;Parent=MsG0180000138.01.T01
Chr1 exon 1943375 1943746 1943375 ID=MsG0180000138.01.T01:exon:6889;Parent=MsG0180000138.01.T01
Chr1 five_prime_UTR 1945275 1945374 1945275 ID=MsG0180000138.01.T01:five_prime_utr;Parent=MsG0180000138.01.T01
Chr1 CDS 1945223 1945274 1945223 ID=MsG0180000138.01.T01:cds;Parent=MsG0180000138.01.T01
Chr1 CDS 1944912 1945084 1944912 ID=MsG0180000138.01.T01:cds;Parent=MsG0180000138.01.T01
Chr1 CDS 1943895 1944055 1943895 ID=MsG0180000138.01.T01:cds;Parent=MsG0180000138.01.T01
Chr1 CDS 1943638 1943746 1943638 ID=MsG0180000138.01.T01:cds;Parent=MsG0180000138.01.T01
Chr1 three_prime_UTR 1943375 1943637 1943375 ID=MsG0180000138.01.T01:three_prime_utr;Parent=MsG0180000138.01.T01
Gene Sequence

>MsG0180000138.01.T01

ATGGGTTCTCTTATGGCAGGATGGGGCTCTTCTTCAATTGATCCTAAATCAGCCATACTTAAAAGGAACCAGTCACTTACAAAAGATGAAATTGATGCTTATTGGAAATCAAAGAAGAAGATAGAGGAAGAACACCTTACAGCAATTTCCAATTTGTCAAAGACTATTCAGGTTGATATTCAACCTCTACTTGCCACTTTCCAGCATGGGCAGATCCAACTTATGACAATTCCAAGTTTGAAATTAATGTTTAGGATGATGATGCAGCCTGATCCTGAAAAGAAGTTTCAGAAGTCAATGACCATGCCCGTGGCCCACGTAAGGGACCCCTTTAATATGAACGTACTTGACACAAATTTGGAGCAACTTATCAACAAAAATGGCTGGTGGACTAAGAGTAACTGGGCATTTCTGAATGAGCCACCAATGATGGAAGCTGCATCCAACAAGTATGCAGCACAGTTTCACGTTGCCAGTTTGGGATCGACAAATTGA

Protein sequence

>MsG0180000138.01.T01

MGSLMAGWGSSSIDPKSAILKRNQSLTKDEIDAYWKSKKKIEEEHLTAISNLSKTIQVDIQPLLATFQHGQIQLMTIPSLKLMFRMMMQPDPEKKFQKSMTMPVAHVRDPFNMNVLDTNLEQLINKNGWWTKSNWAFLNEPPMMEAASNKYAAQFHVASLGSTN*