AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038860.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038860.01.T01 MTR_7g063490 93.023 86 6 0 1 86 1 86 1.09e-46 144
MsG0780038860.01.T01 MTR_4g021330 55.056 89 36 3 1 85 1 89 1.36e-23 85.9
MsG0780038860.01.T01 MTR_2g104750 50.000 86 38 4 1 81 1 86 3.99e-12 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACTTCCAACCGGTGATTC+CGG 0.332515 7:+54235780 MsG0780038860.01.T01:CDS
AATGGAGGGTCTTATACCTT+TGG 0.361131 7:+54235671 None:intergenic
GCACAAATCATTGTTTCATC+TGG 0.387174 7:+54235861 MsG0780038860.01.T01:CDS
GGTGGTAAAGAAGGTGCTAT+AGG 0.442411 7:+54235720 MsG0780038860.01.T01:CDS
AAGAAGGTGCTATAGGATCA+TGG 0.480205 7:+54235727 MsG0780038860.01.T01:CDS
TTGAAACCGACCGGAATCAC+CGG 0.491223 7:-54235790 None:intergenic
CAATACAAAGGTGGTAAAGA+AGG 0.512508 7:+54235711 MsG0780038860.01.T01:CDS
AACATGGTTGGAAGATGAAG+TGG 0.530962 7:-54235820 None:intergenic
ATTATTGTCTTGTAAACCAA+AGG 0.536810 7:-54235687 None:intergenic
AGAAGGTGCTATAGGATCAT+GGG 0.557929 7:+54235728 MsG0780038860.01.T01:CDS
GGTTTGCATTTGAAACCGAC+CGG 0.571028 7:-54235799 None:intergenic
TCATACATGAGACTTCCAAC+CGG 0.604318 7:+54235771 MsG0780038860.01.T01:CDS
TTCCAACCGGTGATTCCGGT+CGG 0.604554 7:+54235784 MsG0780038860.01.T01:CDS
AACCGACCGGAATCACCGGT+TGG 0.622675 7:-54235786 None:intergenic
AGTCTCATGTATGAATATGA+AGG 0.623950 7:-54235762 None:intergenic
AGTAGAAGAAGAAACATGGT+TGG 0.627898 7:-54235832 None:intergenic
CGATGTGTGAGACAATTATG+TGG 0.634825 7:-54235894 None:intergenic
ACAATAATTCAATACAAAGG+TGG 0.639646 7:+54235702 MsG0780038860.01.T01:CDS
AAGACAATAATTCAATACAA+AGG 0.658842 7:+54235699 MsG0780038860.01.T01:CDS
TTGCAGTAGAAGAAGAAACA+TGG 0.697263 7:-54235836 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGACAATAATTCAATACAA+AGG + Chr7:54235699-54235718 MsG0780038860.01.T01:CDS 20.0%
! ACAATAATTCAATACAAAGG+TGG + Chr7:54235702-54235721 MsG0780038860.01.T01:CDS 25.0%
! ATTATTGTCTTGTAAACCAA+AGG - Chr7:54235690-54235709 None:intergenic 25.0%
AGTCTCATGTATGAATATGA+AGG - Chr7:54235765-54235784 None:intergenic 30.0%
AGTAGAAGAAGAAACATGGT+TGG - Chr7:54235835-54235854 None:intergenic 35.0%
CAATACAAAGGTGGTAAAGA+AGG + Chr7:54235711-54235730 MsG0780038860.01.T01:CDS 35.0%
GCACAAATCATTGTTTCATC+TGG + Chr7:54235861-54235880 MsG0780038860.01.T01:CDS 35.0%
TTGCAGTAGAAGAAGAAACA+TGG - Chr7:54235839-54235858 None:intergenic 35.0%
AACATGGTTGGAAGATGAAG+TGG - Chr7:54235823-54235842 None:intergenic 40.0%
CGATGTGTGAGACAATTATG+TGG - Chr7:54235897-54235916 None:intergenic 40.0%
TCATACATGAGACTTCCAAC+CGG + Chr7:54235771-54235790 MsG0780038860.01.T01:CDS 40.0%
!! AAGAAGGTGCTATAGGATCA+TGG + Chr7:54235727-54235746 MsG0780038860.01.T01:CDS 40.0%
!! AGAAGGTGCTATAGGATCAT+GGG + Chr7:54235728-54235747 MsG0780038860.01.T01:CDS 40.0%
! GGTTTGCATTTGAAACCGAC+CGG - Chr7:54235802-54235821 None:intergenic 45.0%
!! GGTGGTAAAGAAGGTGCTAT+AGG + Chr7:54235720-54235739 MsG0780038860.01.T01:CDS 45.0%
AGACTTCCAACCGGTGATTC+CGG + Chr7:54235780-54235799 MsG0780038860.01.T01:CDS 50.0%
TTGAAACCGACCGGAATCAC+CGG - Chr7:54235793-54235812 None:intergenic 50.0%
TTCCAACCGGTGATTCCGGT+CGG + Chr7:54235784-54235803 MsG0780038860.01.T01:CDS 55.0%
AACCGACCGGAATCACCGGT+TGG - Chr7:54235789-54235808 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 54235672 54235932 54235672 ID=MsG0780038860.01;Name=MsG0780038860.01
Chr7 mRNA 54235672 54235932 54235672 ID=MsG0780038860.01.T01;Parent=MsG0780038860.01;Name=MsG0780038860.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|0|1|0|86
Chr7 exon 54235672 54235932 54235672 ID=MsG0780038860.01.T01:exon:15286;Parent=MsG0780038860.01.T01
Chr7 CDS 54235672 54235932 54235672 ID=MsG0780038860.01.T01:cds;Parent=MsG0780038860.01.T01
Gene Sequence

>MsG0780038860.01.T01

ATGGAGGGTCTTATACCTTTGGTTTACAAGACAATAATTCAATACAAAGGTGGTAAAGAAGGTGCTATAGGATCATGGGTTTGTGATTCACCTTCATATTCATACATGAGACTTCCAACCGGTGATTCCGGTCGGTTTCAAATGCAAACCACTTCATCTTCCAACCATGTTTCTTCTTCTACTGCAAGTGCACAAATCATTGTTTCATCTGGTGTTCAATCTCCACATAATTGTCTCACACATCGTCGAATCGCTGCATGA

Protein sequence

>MsG0780038860.01.T01

MEGLIPLVYKTIIQYKGGKEGAIGSWVCDSPSYSYMRLPTGDSGRFQMQTTSSSNHVSSSTASAQIIVSSGVQSPHNCLTHRRIAA*