AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024144.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024144.01.T01 MTR_5g006360 70.779 154 31 2 52 205 1 140 3.37e-69 207
MsG0580024144.01.T01 MTR_5g006370 60.000 105 38 1 50 154 2 102 6.03e-40 133
MsG0580024144.01.T01 MTR_5g006370 60.000 105 38 1 50 154 2 102 7.15e-40 133
MsG0580024144.01.T01 MTR_5g006370 57.692 78 29 1 77 154 1 74 3.54e-25 95.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024144.01.T01 AT2G32090 68.000 100 31 1 55 154 2 100 1.55e-48 155
MsG0580024144.01.T01 AT2G32090 70.513 78 22 1 77 154 2 78 2.36e-36 123

Find 60 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCAAGTTCGGAGAGTTTA+AGG 0.169316 5:-1310601 MsG0580024144.01.T01:CDS
AAAAGAAAACCTTGAGAATT+TGG 0.172759 5:+1310406 None:intergenic
AGTTACCTCTTCATAGAATT+TGG 0.196394 5:+1310749 None:intergenic
TTGGAGGAAAGATTGGAAAT+TGG 0.234528 5:+1310425 None:intergenic
TGGAGGAAAGATTGGAAATT+GGG 0.285778 5:+1310426 None:intergenic
GTGGCACTCCATGGACCTTC+AGG 0.291137 5:+1310507 None:intergenic
ACACAAACTATAGTAAAATT+AGG 0.312173 5:+1312969 None:intergenic
TGAGAATTTGGAGGAAAGAT+TGG 0.329429 5:+1310418 None:intergenic
AGAGAAAAGTGAAGCTGAAA+TGG 0.368788 5:-1310826 MsG0580024144.01.T01:CDS
CGGTGGACTCTCGAGATATA+TGG 0.373213 5:+1310787 None:intergenic
TTTGAGAAATCTCTGCCTAA+TGG 0.386060 5:-1309805 MsG0580024144.01.T01:CDS
GATGTTCTTAAATGACAATA+AGG 0.392166 5:+1309552 None:intergenic
AACCTTAAACTCTCCGAACT+TGG 0.393413 5:+1310599 None:intergenic
TAGAAGGGTCGGTCGACTAT+AGG 0.403222 5:-1313022 MsG0580024144.01.T01:CDS
TCGGAGAGTTTAAGGTTGTA+TGG 0.410903 5:-1310593 MsG0580024144.01.T01:CDS
CTTCAGGGAGATTGTTGGAT+GGG 0.413649 5:+1310523 None:intergenic
AGATGGGAAGGGTCTTTAAC+AGG 0.425313 5:+1310480 None:intergenic
TGTTGTAAGTCTGCTTGCAC+AGG 0.453222 5:+1312999 None:intergenic
TTAATTGTATATGCAGGTAA+TGG 0.459390 5:-1313221 None:intergenic
TGAAGATACAATGAAGAAGA+TGG 0.469893 5:+1310561 None:intergenic
GAAGTAGAAAGTCCCAAGTT+CGG 0.482224 5:-1310612 MsG0580024144.01.T01:CDS
CCTTCAGGGAGATTGTTGGA+TGG 0.483246 5:+1310522 None:intergenic
GATGATGGCCTCTAGGAAGA+TGG 0.486052 5:+1310463 None:intergenic
ATCTTTCCTCCAAATTCTCA+AGG 0.486071 5:-1310415 MsG0580024144.01.T01:CDS
TGGACCTTCAGGGAGATTGT+TGG 0.488258 5:+1310518 None:intergenic
TACTACATAGTCTTACCATC+AGG 0.496646 5:+1309754 None:intergenic
ATCTGTAGGTAATGGCTTGG+AGG 0.496826 5:-1309646 MsG0580024144.01.T01:intron
GGAGGAAAGATTGGAAATTG+GGG 0.506081 5:+1310427 None:intergenic
AAGCAGAGATGATGGCCTCT+AGG 0.507231 5:+1310456 None:intergenic
GGACAGAGAAGCAGAGATGA+TGG 0.508377 5:+1310448 None:intergenic
GCAAGCAGACTTACAACAAA+AGG 0.511066 5:-1312994 MsG0580024144.01.T01:CDS
TCAATCTGTAGGTAATGGCT+TGG 0.513155 5:-1309649 MsG0580024144.01.T01:intron
TTTCATTTCATATGGTCCTA+GGG 0.518752 5:-1310386 MsG0580024144.01.T01:intron
TGTATATGCAGGTAATGGCT+TGG 0.518965 5:-1313216 None:intergenic
TGGCACTCCATGGACCTTCA+GGG 0.526229 5:+1310508 None:intergenic
GGCAAGGCGGTTTACATCGG+TGG 0.530632 5:+1310770 None:intergenic
ACCTTAAACTCTCCGAACTT+GGG 0.532859 5:+1310600 None:intergenic
ATGATGGCCTCTAGGAAGAT+GGG 0.534418 5:+1310464 None:intergenic
TGGCTTGGAGGTTGCAAGCA+AGG 0.537704 5:-1309634 MsG0580024144.01.T01:CDS
ACAATCTCCCTGAAGGTCCA+TGG 0.539494 5:-1310515 MsG0580024144.01.T01:CDS
AGAAAACCTTGAGAATTTGG+AGG 0.542787 5:+1310409 None:intergenic
ACAGGAGAGGTGGCACTCCA+TGG 0.546931 5:+1310498 None:intergenic
ATGCATATCAAAGTAACTCT+AGG 0.559770 5:+1309605 None:intergenic
ATATGCAGGTAATGGCTTGG+AGG 0.578557 5:-1313213 MsG0580024144.01.T01:CDS
TGGCCTCTAGGAAGATGGGA+AGG 0.592110 5:+1310468 None:intergenic
TTTGGCAAGGCGGTTTACAT+CGG 0.596297 5:+1310767 None:intergenic
CCTTGCCAAATTCTATGAAG+AGG 0.598216 5:-1310754 MsG0580024144.01.T01:intron
GCAATTACAGCAGCATCTAG+AGG 0.599720 5:+1309496 None:intergenic
CCTCTTCATAGAATTTGGCA+AGG 0.604080 5:+1310754 None:intergenic
CAATTACAGCAGCATCTAGA+GGG 0.610460 5:+1309497 None:intergenic
CCATCCAACAATCTCCCTGA+AGG 0.629938 5:-1310522 MsG0580024144.01.T01:CDS
GGCCTCTAGGAAGATGGGAA+GGG 0.632526 5:+1310469 None:intergenic
CTTCATAGAATTTGGCAAGG+CGG 0.637595 5:+1310757 None:intergenic
ACTACATAGTCTTACCATCA+GGG 0.643829 5:+1309755 None:intergenic
AGGGTCTTTAACAGGAGAGG+TGG 0.645849 5:+1310488 None:intergenic
GGAAGGGTCTTTAACAGGAG+AGG 0.653406 5:+1310485 None:intergenic
TATAGTTTGTGTGTTGCCTG+CGG 0.662507 5:-1312958 MsG0580024144.01.T01:intron
GACCCTTCCCATCTTCCTAG+AGG 0.663206 5:-1310471 MsG0580024144.01.T01:CDS
TTACAGCAGCATCTAGAGGG+AGG 0.687200 5:+1309500 None:intergenic
TGGATGGGTTGCGCTCGATG+AGG 0.700869 5:+1310538 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTTTTTATAAATTA+TGG + Chr5:1310975-1310994 None:intergenic 0.0%
!! AAAATTGAATATAAATAAGA+AGG + Chr5:1311195-1311214 None:intergenic 10.0%
!! TTAATAAAAGTTAATGTATT+TGG + Chr5:1312323-1312342 None:intergenic 10.0%
!! TTACTAATAATAATCTTAAA+AGG + Chr5:1311153-1311172 None:intergenic 10.0%
!! TTCTATTTCATTTAAAAATT+TGG + Chr5:1311822-1311841 None:intergenic 10.0%
!!! TCTATTTTTTTTATTTTGAA+TGG - Chr5:1310830-1310849 MsG0580024144.01.T01:CDS 10.0%
!!! TTTTTTATATCAGTAAAATT+TGG - Chr5:1312950-1312969 MsG0580024144.01.T01:intron 10.0%
!!! TTTTTTTTTTTAATAGTGAT+CGG - Chr5:1311501-1311520 MsG0580024144.01.T01:intron 10.0%
!! ACAAAAGTAAAAAAATATCT+TGG - Chr5:1311084-1311103 MsG0580024144.01.T01:intron 15.0%
!! ATACTATAATACTATATGTT+TGG - Chr5:1312004-1312023 MsG0580024144.01.T01:intron 15.0%
!! TTGATTATGTAAAATTGATT+TGG - Chr5:1312499-1312518 MsG0580024144.01.T01:intron 15.0%
!!! AGAAATTAAGATTTTTTTCA+AGG + Chr5:1310488-1310507 None:intergenic 15.0%
!!! GTTTTTTTTTTAATAGTGAT+CGG - Chr5:1311679-1311698 MsG0580024144.01.T01:intron 15.0%
!!! TAATAACAGATTTTGTTTTA+TGG + Chr5:1310005-1310024 None:intergenic 15.0%
!!! TAATATGATTTTCAGATATT+TGG - Chr5:1312036-1312055 MsG0580024144.01.T01:intron 15.0%
!!! TAGCTTTATTTTATGAATAA+AGG + Chr5:1313209-1313228 None:intergenic 15.0%
!!! TTTTTTTTTTAATAGTGATC+GGG - Chr5:1311502-1311521 MsG0580024144.01.T01:intron 15.0%
!! AATCTTAATTTCTCTTATTC+TGG - Chr5:1310495-1310514 MsG0580024144.01.T01:CDS 20.0%
!! ACACAAACTATAGTAAAATT+AGG + Chr5:1309712-1309731 None:intergenic 20.0%
!! AGTCAAAGTTAATTAAGATA+TGG + Chr5:1310031-1310050 None:intergenic 20.0%
!! ATGTAAAATTGTAAGCATTT+CGG - Chr5:1310719-1310738 MsG0580024144.01.T01:intron 20.0%
!! GTAAAAAAATATCTTGGAAA+TGG - Chr5:1311090-1311109 MsG0580024144.01.T01:intron 20.0%
!! TACAAAAATTAGTGTATGTT+TGG + Chr5:1312733-1312752 None:intergenic 20.0%
!! TGCATAAATGAAAATATGTT+TGG + Chr5:1311322-1311341 None:intergenic 20.0%
!! TTCATAATTAAGTGAGTATT+TGG - Chr5:1312444-1312463 MsG0580024144.01.T01:intron 20.0%
!!! AAACATATTTTCATTTATGC+AGG - Chr5:1311321-1311340 MsG0580024144.01.T01:intron 20.0%
!!! AAAGCTAAAAAATTAGTTTG+AGG - Chr5:1312593-1312612 MsG0580024144.01.T01:intron 20.0%
!!! AACATATTTTCATTTATGCA+GGG - Chr5:1311322-1311341 MsG0580024144.01.T01:intron 20.0%
!!! AACCTTTTAAGATTTAGAAA+TGG - Chr5:1311119-1311138 MsG0580024144.01.T01:intron 20.0%
!!! AAGGTGACAAAATTTATTTT+TGG - Chr5:1312471-1312490 MsG0580024144.01.T01:intron 20.0%
!!! AATATGAAGTTTTGTATTTG+AGG - Chr5:1312780-1312799 MsG0580024144.01.T01:intron 20.0%
!!! ACATTTTCAACAATTTCAAT+TGG - Chr5:1310054-1310073 MsG0580024144.01.T01:intron 20.0%
!!! ATATGAAGTTTTGTATTTGA+GGG - Chr5:1312781-1312800 MsG0580024144.01.T01:intron 20.0%
!!! ATGAAAATTTCTTTTAGCTT+TGG + Chr5:1309898-1309917 None:intergenic 20.0%
!!! ATTCAAATTTATTCCTCTTT+TGG + Chr5:1310888-1310907 None:intergenic 20.0%
!!! ATTGTTATTATTCTCTGAAT+TGG - Chr5:1312974-1312993 MsG0580024144.01.T01:CDS 20.0%
!!! ATTTTTTTCAAAGAAGAGAA+AGG - Chr5:1311776-1311795 MsG0580024144.01.T01:intron 20.0%
!!! CATACACTAATTTTTGTATA+AGG - Chr5:1312735-1312754 MsG0580024144.01.T01:intron 20.0%
!!! CTCACTTAATTATGAAATTT+TGG + Chr5:1312440-1312459 None:intergenic 20.0%
!!! TATATTTATTTAAGACACGA+GGG - Chr5:1312356-1312375 MsG0580024144.01.T01:intron 20.0%
!!! TTAAGTTAGAATAGTTTTAC+TGG - Chr5:1312637-1312656 MsG0580024144.01.T01:intron 20.0%
!!! TTATATTTATTTAAGACACG+AGG - Chr5:1312355-1312374 MsG0580024144.01.T01:intron 20.0%
!!! TTTAGAATATCAATAATGTG+AGG - Chr5:1310932-1310951 MsG0580024144.01.T01:intron 20.0%
!!! TTTTTAGTCTAATATCCTAT+TGG + Chr5:1311405-1311424 None:intergenic 20.0%
!!! TTTTTTTTTAATAGTGATCG+GGG - Chr5:1311503-1311522 MsG0580024144.01.T01:intron 20.0%
! AAAAGAAAACCTTGAGAATT+TGG + Chr5:1312275-1312294 None:intergenic 25.0%
! AATTATAGAAAAAGTGGGAT+AGG + Chr5:1310135-1310154 None:intergenic 25.0%
! ATCTGTTGTTAATTGTATCA+AGG - Chr5:1310601-1310620 MsG0580024144.01.T01:CDS 25.0%
! ATTACCTACAGATTGATATT+AGG + Chr5:1313025-1313044 None:intergenic 25.0%
! CACCATTTCTAAATCTTAAA+AGG + Chr5:1311124-1311143 None:intergenic 25.0%
! CAGTCAGATTCTATAATAAT+TGG + Chr5:1310580-1310599 None:intergenic 25.0%
! GAAATTAACTCTAAACAATC+TGG - Chr5:1312678-1312697 MsG0580024144.01.T01:intron 25.0%
! GATATTAATTCTCATGTAGA+GGG + Chr5:1310678-1310697 None:intergenic 25.0%
! GATGTTCTTAAATGACAATA+AGG + Chr5:1313129-1313148 None:intergenic 25.0%
! TAATATCAATCTGTAGGTAA+TGG - Chr5:1313024-1313043 MsG0580024144.01.T01:intron 25.0%
! TAATATGATCCTAGAATTTG+CGG + Chr5:1310097-1310116 None:intergenic 25.0%
! TAGCCAATTATAGAAAAAGT+GGG + Chr5:1310140-1310159 None:intergenic 25.0%
! TGAATGATCAGTGATTATTA+TGG - Chr5:1312534-1312553 MsG0580024144.01.T01:intron 25.0%
! TGATATTAATTCTCATGTAG+AGG + Chr5:1310679-1310698 None:intergenic 25.0%
! TTACCTACAGATTGATATTA+GGG + Chr5:1313024-1313043 None:intergenic 25.0%
! TTAGCCAATTATAGAAAAAG+TGG + Chr5:1310141-1310160 None:intergenic 25.0%
!! AAAACTTTGCTAATTTGTCA+AGG + Chr5:1311545-1311564 None:intergenic 25.0%
!! AAACTTTGCTAATTTGTCAA+GGG + Chr5:1311544-1311563 None:intergenic 25.0%
!! ACATATTTTCATTTATGCAG+GGG - Chr5:1311323-1311342 MsG0580024144.01.T01:intron 25.0%
!! CTTTTTCTATAATTGGCTAA+AGG - Chr5:1310141-1310160 MsG0580024144.01.T01:intron 25.0%
!! GGTTTTCTTTTCATTTCATA+TGG - Chr5:1312284-1312303 MsG0580024144.01.T01:intron 25.0%
!! TACAAGTTGACAAAATGAAA+GGG + Chr5:1309774-1309793 None:intergenic 25.0%
!! TGAATGGTTTTCAAAGTTAA+AGG + Chr5:1310185-1310204 None:intergenic 25.0%
!! TTTTCAGATATTTGGATTTG+AGG - Chr5:1312044-1312063 MsG0580024144.01.T01:intron 25.0%
!!! AATTTTAACTACTCCAAAAG+TGG - Chr5:1312705-1312724 MsG0580024144.01.T01:intron 25.0%
!!! TATCCCACTTTTTCTATAAT+TGG - Chr5:1310134-1310153 MsG0580024144.01.T01:intron 25.0%
!!! TATGAAGTTTTGTATTTGAG+GGG - Chr5:1312782-1312801 MsG0580024144.01.T01:intron 25.0%
!!! TTTGTTTGTTTGTTTTATGC+AGG - Chr5:1312833-1312852 MsG0580024144.01.T01:intron 25.0%
AGCTTCAGAATCAGAAATTT+GGG - Chr5:1310372-1310391 MsG0580024144.01.T01:intron 30.0%
AGTTACCTCTTCATAGAATT+TGG + Chr5:1311932-1311951 None:intergenic 30.0%
ATAGAAGATTGAAGACTTGT+TGG + Chr5:1310258-1310277 None:intergenic 30.0%
ATGCATATCAAAGTAACTCT+AGG + Chr5:1313076-1313095 None:intergenic 30.0%
ATTGGTCAATAGTATTGTCT+TGG - Chr5:1310549-1310568 MsG0580024144.01.T01:CDS 30.0%
CAGTATCATTAATCAAGACT+AGG + Chr5:1309600-1309619 None:intergenic 30.0%
GTATGTGTAAAGACTAGAAT+TGG + Chr5:1310866-1310885 None:intergenic 30.0%
TACCTACAGATTGATATTAG+GGG + Chr5:1313023-1313042 None:intergenic 30.0%
TAGCTTCAGAATCAGAAATT+TGG - Chr5:1310371-1310390 MsG0580024144.01.T01:intron 30.0%
TCTCTGAATTGGTTGTTTAT+TGG - Chr5:1312985-1313004 MsG0580024144.01.T01:CDS 30.0%
TGAAGATACAATGAAGAAGA+TGG + Chr5:1312120-1312139 None:intergenic 30.0%
TTTCATTTCATATGGTCCTA+GGG - Chr5:1312292-1312311 MsG0580024144.01.T01:intron 30.0%
! ACAAGTTGACAAAATGAAAG+GGG + Chr5:1309773-1309792 None:intergenic 30.0%
! CAATTGGAAATTTGTTCGAA+CGG - Chr5:1310070-1310089 MsG0580024144.01.T01:intron 30.0%
! CTACAAGTTGACAAAATGAA+AGG + Chr5:1309775-1309794 None:intergenic 30.0%
! GTTTTCAAAGTTAAAGGATC+TGG + Chr5:1310179-1310198 None:intergenic 30.0%
! TTTTCATTTCATATGGTCCT+AGG - Chr5:1312291-1312310 MsG0580024144.01.T01:intron 30.0%
!! ACTTGTTTGATTTTGCCATT+AGG + Chr5:1312891-1312910 None:intergenic 30.0%
!! TAAGTGAGTATTTGGTTCTA+AGG - Chr5:1312452-1312471 MsG0580024144.01.T01:intron 30.0%
!!! GGTGGCTTTATTTTTTTTCA+AGG - Chr5:1309747-1309766 MsG0580024144.01.T01:intron 30.0%
!!! GTTTGTTTTATGCAGGATAA+GGG - Chr5:1312840-1312859 MsG0580024144.01.T01:intron 30.0%
!!! TGTTTGTTTTATGCAGGATA+AGG - Chr5:1312839-1312858 MsG0580024144.01.T01:intron 30.0%
AAAATGCCTACCAAGGAATA+TGG - Chr5:1309955-1309974 MsG0580024144.01.T01:intron 35.0%
AAATGAAGCAACAATAGCCT+TGG + Chr5:1310771-1310790 None:intergenic 35.0%
AACATTAATTAAGACACCCG+CGG - Chr5:1309924-1309943 MsG0580024144.01.T01:intron 35.0%
AACCCCTAATATCAATCTGT+AGG - Chr5:1313018-1313037 MsG0580024144.01.T01:CDS 35.0%
ACACCAAATTAGTAACTAGG+AGG + Chr5:1309512-1309531 None:intergenic 35.0%
ACCGAATGATGTTCTCAAAA+TGG - Chr5:1310399-1310418 MsG0580024144.01.T01:CDS 35.0%
ACGACACCAAATTAGTAACT+AGG + Chr5:1309515-1309534 None:intergenic 35.0%
ACTCCTCCTAGTTACTAATT+TGG - Chr5:1309506-1309525 MsG0580024144.01.T01:three_prime_UTR 35.0%
AGAAAACCTTGAGAATTTGG+AGG + Chr5:1312272-1312291 None:intergenic 35.0%
AGAGAAAAGTGAAGCTGAAA+TGG - Chr5:1311852-1311871 MsG0580024144.01.T01:intron 35.0%
AGTTCTACAAGTCTTCAAGT+AGG - Chr5:1310205-1310224 MsG0580024144.01.T01:intron 35.0%
ATCTTTCCTCCAAATTCTCA+AGG - Chr5:1312263-1312282 MsG0580024144.01.T01:intron 35.0%
ATTTCACGTTTCCATTGCTA+TGG - Chr5:1310290-1310309 MsG0580024144.01.T01:intron 35.0%
CAATTAAATGTGGAGAAGTG+GGG + Chr5:1311370-1311389 None:intergenic 35.0%
CTTGTAGCATTGTTTCCATT+AGG - Chr5:1309788-1309807 MsG0580024144.01.T01:CDS 35.0%
GAAGACTTGTAGAACTTGAA+TGG + Chr5:1310201-1310220 None:intergenic 35.0%
GAGGTAAAAGCTTCAAAATC+TGG - Chr5:1312612-1312631 MsG0580024144.01.T01:intron 35.0%
GCAATTAAATGTGGAGAAGT+GGG + Chr5:1311371-1311390 None:intergenic 35.0%
GTCTTTACACATACCAAAAG+AGG - Chr5:1310872-1310891 MsG0580024144.01.T01:intron 35.0%
GTGAACAAATTACCTCCTAA+TGG + Chr5:1309806-1309825 None:intergenic 35.0%
TGAGAATTTGGAGGAAAGAT+TGG + Chr5:1312263-1312282 None:intergenic 35.0%
TGGAGGAAAGATTGGAAATT+GGG + Chr5:1312255-1312274 None:intergenic 35.0%
TGTTCTCAAAATGGCCATAT+TGG - Chr5:1310408-1310427 MsG0580024144.01.T01:CDS 35.0%
TTGGAGGAAAGATTGGAAAT+TGG + Chr5:1312256-1312275 None:intergenic 35.0%
TTGTTAGAAAATGCCTACCA+AGG - Chr5:1309948-1309967 MsG0580024144.01.T01:intron 35.0%
TTTGAGAAATCTCTGCCTAA+TGG - Chr5:1312873-1312892 MsG0580024144.01.T01:intron 35.0%
! AATTTCTCTTATTCTGGCTG+AGG - Chr5:1310501-1310520 MsG0580024144.01.T01:CDS 35.0%
! ACTACATAGTCTTACCATCA+GGG + Chr5:1312926-1312945 None:intergenic 35.0%
! CTATGACAAGTTTGCTTCAT+AGG + Chr5:1310354-1310373 None:intergenic 35.0%
! CTATTGTTGCTTCATTTGCA+AGG - Chr5:1310774-1310793 MsG0580024144.01.T01:CDS 35.0%
! TAATTTGTCAAGGGAATTGG+CGG + Chr5:1311535-1311554 None:intergenic 35.0%
! TACTACATAGTCTTACCATC+AGG + Chr5:1312927-1312946 None:intergenic 35.0%
! TCAAAATGGCCATATTGGTT+GGG - Chr5:1310413-1310432 MsG0580024144.01.T01:CDS 35.0%
! TGCTAATTTGTCAAGGGAAT+TGG + Chr5:1311538-1311557 None:intergenic 35.0%
!! AAGACTAGGTTGATGATAGT+TGG + Chr5:1309586-1309605 None:intergenic 35.0%
!! GCCATTTTGAGAACATCATT+CGG + Chr5:1310403-1310422 None:intergenic 35.0%
!! TGTATGTTTGGTTCCACTTT+TGG + Chr5:1312721-1312740 None:intergenic 35.0%
!! TGTCGGGTTTTTGTTTAGAA+GGG - Chr5:1309641-1309660 MsG0580024144.01.T01:CDS 35.0%
!! TTGTCGGGTTTTTGTTTAGA+AGG - Chr5:1309640-1309659 MsG0580024144.01.T01:CDS 35.0%
!!! TTCTTTTAGCTTTGGCGATA+AGG + Chr5:1309890-1309909 None:intergenic 35.0%
AACCTTAAACTCTCCGAACT+TGG + Chr5:1312082-1312101 None:intergenic 40.0%
ACATTAATTAAGACACCCGC+GGG - Chr5:1309925-1309944 MsG0580024144.01.T01:intron 40.0%
ACCTTAAACTCTCCGAACTT+GGG + Chr5:1312081-1312100 None:intergenic 40.0%
AGAGCTCACGCAATTAAATG+TGG + Chr5:1311380-1311399 None:intergenic 40.0%
AGCGTTAATAAACTGCCGAA+GGG + Chr5:1310328-1310347 None:intergenic 40.0%
AGGTATCCATATTCCTTGGT+AGG + Chr5:1309964-1309983 None:intergenic 40.0%
ATTTCATATGGTCCTAGGGT+CGG - Chr5:1312296-1312315 MsG0580024144.01.T01:intron 40.0%
CAATTACAGCAGCATCTAGA+GGG + Chr5:1313184-1313203 None:intergenic 40.0%
CCTCTTCATAGAATTTGGCA+AGG + Chr5:1311927-1311946 None:intergenic 40.0%
CCTTGCCAAATTCTATGAAG+AGG - Chr5:1311924-1311943 MsG0580024144.01.T01:intron 40.0%
CGCAATTAAATGTGGAGAAG+TGG + Chr5:1311372-1311391 None:intergenic 40.0%
CGCTACTTGTGATTTCTTGT+CGG - Chr5:1309624-1309643 MsG0580024144.01.T01:CDS 40.0%
CTAAGAAACCCCAACCAATA+TGG + Chr5:1310425-1310444 None:intergenic 40.0%
CTCAAAATGGCCATATTGGT+TGG - Chr5:1310412-1310431 MsG0580024144.01.T01:CDS 40.0%
CTCGAGATGGTGTCTTAAAT+TGG - Chr5:1310531-1310550 MsG0580024144.01.T01:CDS 40.0%
CTTCATAGAATTTGGCAAGG+CGG + Chr5:1311924-1311943 None:intergenic 40.0%
GAAGTAGAAAGTCCCAAGTT+CGG - Chr5:1312066-1312085 MsG0580024144.01.T01:intron 40.0%
GCAAGCAGACTTACAACAAA+AGG - Chr5:1309684-1309703 MsG0580024144.01.T01:intron 40.0%
GCTACTTGTGATTTCTTGTC+GGG - Chr5:1309625-1309644 MsG0580024144.01.T01:CDS 40.0%
GGAGGAAAGATTGGAAATTG+GGG + Chr5:1312254-1312273 None:intergenic 40.0%
GGTCAGATGCATGTAAAACT+GGG - Chr5:1312555-1312574 MsG0580024144.01.T01:intron 40.0%
GTAGCATTGTTTCCATTAGG+AGG - Chr5:1309791-1309810 MsG0580024144.01.T01:CDS 40.0%
TAGCGTTAATAAACTGCCGA+AGG + Chr5:1310329-1310348 None:intergenic 40.0%
TGGTCAGATGCATGTAAAAC+TGG - Chr5:1312554-1312573 MsG0580024144.01.T01:intron 40.0%
TTGTTGGCGAAGCTAAGTTA+TGG + Chr5:1310242-1310261 None:intergenic 40.0%
! ATTTTCATTTATGCAGGGGC+TGG - Chr5:1311327-1311346 MsG0580024144.01.T01:intron 40.0%
! CAAAATGGCCATATTGGTTG+GGG - Chr5:1310414-1310433 MsG0580024144.01.T01:CDS 40.0%
! CAATTTAAGACACCATCTCG+AGG + Chr5:1310533-1310552 None:intergenic 40.0%
! GTTTTCTTCTTTGACCCTGA+TGG - Chr5:1312909-1312928 MsG0580024144.01.T01:intron 40.0%
! TATAGTTTGTGTGTTGCCTG+CGG - Chr5:1309720-1309739 MsG0580024144.01.T01:intron 40.0%
! TCAATCTGTAGGTAATGGCT+TGG - Chr5:1313029-1313048 MsG0580024144.01.T01:intron 40.0%
! TCGGAGAGTTTAAGGTTGTA+TGG - Chr5:1312085-1312104 MsG0580024144.01.T01:intron 40.0%
! TTTTCATTTATGCAGGGGCT+GGG - Chr5:1311328-1311347 MsG0580024144.01.T01:intron 40.0%
!! AGCATTTCGGTTCTAAGTCT+TGG - Chr5:1310732-1310751 MsG0580024144.01.T01:intron 40.0%
!! GCATTTCGGTTCTAAGTCTT+GGG - Chr5:1310733-1310752 MsG0580024144.01.T01:intron 40.0%
!! GGGTTTTTGTTTAGAAGGGT+CGG - Chr5:1309645-1309664 MsG0580024144.01.T01:intron 40.0%
AATGTATTTGGTCCGACCCT+AGG + Chr5:1312311-1312330 None:intergenic 45.0%
AGATGGGAAGGGTCTTTAAC+AGG + Chr5:1312201-1312220 None:intergenic 45.0%
CTTCAGGGAGATTGTTGGAT+GGG + Chr5:1312158-1312177 None:intergenic 45.0%
GATATTAGGGGTTCAGCTCA+GGG + Chr5:1313011-1313030 None:intergenic 45.0%
GCAATTACAGCAGCATCTAG+AGG + Chr5:1313185-1313204 None:intergenic 45.0%
GGACATCAAAGTAGATGACG+AGG + Chr5:1309984-1310003 None:intergenic 45.0%
TCCCAAGTTCGGAGAGTTTA+AGG - Chr5:1312077-1312096 MsG0580024144.01.T01:intron 45.0%
TGATATTAGGGGTTCAGCTC+AGG + Chr5:1313012-1313031 None:intergenic 45.0%
TGTTGTAAGTCTGCTTGCAC+AGG + Chr5:1309682-1309701 None:intergenic 45.0%
TTTCATTTATGCAGGGGCTG+GGG - Chr5:1311329-1311348 MsG0580024144.01.T01:intron 45.0%
TTTGGCAAGGCGGTTTACAT+CGG + Chr5:1311914-1311933 None:intergenic 45.0%
! AGGCATTTTCTAACAACCCG+CGG + Chr5:1309944-1309963 None:intergenic 45.0%
! ATCTGTAGGTAATGGCTTGG+AGG - Chr5:1313032-1313051 MsG0580024144.01.T01:intron 45.0%
! ATGATGGCCTCTAGGAAGAT+GGG + Chr5:1312217-1312236 None:intergenic 45.0%
! GACGAGGTATCCATATTCCT+TGG + Chr5:1309968-1309987 None:intergenic 45.0%
! TCTTATTCTGGCTGAGGCAA+TGG - Chr5:1310507-1310526 MsG0580024144.01.T01:CDS 45.0%
!! TTCGGTTCTAAGTCTTGGGT+GGG - Chr5:1310737-1310756 MsG0580024144.01.T01:intron 45.0%
!! TTTCGGTTCTAAGTCTTGGG+TGG - Chr5:1310736-1310755 MsG0580024144.01.T01:intron 45.0%
!!! TTTTATATGATATAAAAAAT+TGG + Chr5:1311040-1311059 None:intergenic 5.0%
AATAAAGCCACCCAAACCGC+AGG + Chr5:1309739-1309758 None:intergenic 50.0%
ACAATCTCCCTGAAGGTCCA+TGG - Chr5:1312163-1312182 MsG0580024144.01.T01:intron 50.0%
AGGGTCTTTAACAGGAGAGG+TGG + Chr5:1312193-1312212 None:intergenic 50.0%
CCATCCAACAATCTCCCTGA+AGG - Chr5:1312156-1312175 MsG0580024144.01.T01:intron 50.0%
CCTTCAGGGAGATTGTTGGA+TGG + Chr5:1312159-1312178 None:intergenic 50.0%
GGAAGGGTCTTTAACAGGAG+AGG + Chr5:1312196-1312215 None:intergenic 50.0%
GGACAGAGAAGCAGAGATGA+TGG + Chr5:1312233-1312252 None:intergenic 50.0%
TAACGCTGTCGCCATAGCAA+TGG + Chr5:1310304-1310323 None:intergenic 50.0%
TAGAAGGGTCGGTCGACTAT+AGG - Chr5:1309656-1309675 MsG0580024144.01.T01:intron 50.0%
TCGAACGGTCCGCAAATTCT+AGG - Chr5:1310085-1310104 MsG0580024144.01.T01:intron 50.0%
TGGACCTTCAGGGAGATTGT+TGG + Chr5:1312163-1312182 None:intergenic 50.0%
TTACAGCAGCATCTAGAGGG+AGG + Chr5:1313181-1313200 None:intergenic 50.0%
TTGGGTGGGATTTGTCTCCA+AGG - Chr5:1310751-1310770 MsG0580024144.01.T01:intron 50.0%
TTGTGTGTTGCCTGCGGTTT+GGG - Chr5:1309726-1309745 MsG0580024144.01.T01:intron 50.0%
TTTGTGTGTTGCCTGCGGTT+TGG - Chr5:1309725-1309744 MsG0580024144.01.T01:intron 50.0%
! AAGCAGAGATGATGGCCTCT+AGG + Chr5:1312225-1312244 None:intergenic 50.0%
! ATGTGGAGAAGTGGGGTATC+CGG + Chr5:1311363-1311382 None:intergenic 50.0%
! CGGTGGACTCTCGAGATATA+TGG + Chr5:1311894-1311913 None:intergenic 50.0%
! GATGATGGCCTCTAGGAAGA+TGG + Chr5:1312218-1312237 None:intergenic 50.0%
! GGCATTTTCTAACAACCCGC+GGG + Chr5:1309943-1309962 None:intergenic 50.0%
! TTGCGTGAGCTCTAGCCAAT+AGG - Chr5:1311387-1311406 MsG0580024144.01.T01:intron 50.0%
GACCCTTCCCATCTTCCTAG+AGG - Chr5:1312207-1312226 MsG0580024144.01.T01:intron 55.0%
TGGCACTCCATGGACCTTCA+GGG + Chr5:1312173-1312192 None:intergenic 55.0%
TGGCGACAGCGTTATCCCTT+CGG - Chr5:1310310-1310329 MsG0580024144.01.T01:intron 55.0%
! GGCCTCTAGGAAGATGGGAA+GGG + Chr5:1312212-1312231 None:intergenic 55.0%
! TGGCCTCTAGGAAGATGGGA+AGG + Chr5:1312213-1312232 None:intergenic 55.0%
! TGGCTTGGAGGTTGCAAGCA+AGG - Chr5:1313044-1313063 MsG0580024144.01.T01:intron 55.0%
! TGTGGAGAAGTGGGGTATCC+GGG + Chr5:1311362-1311381 None:intergenic 55.0%
ACAGGAGAGGTGGCACTCCA+TGG + Chr5:1312183-1312202 None:intergenic 60.0%
AGGGGCTGGGGTTCAAATCC+CGG - Chr5:1311341-1311360 MsG0580024144.01.T01:intron 60.0%
CTGAGGCAATGGCCTCGAGA+TGG - Chr5:1310518-1310537 MsG0580024144.01.T01:CDS 60.0%
GGCAAGGCGGTTTACATCGG+TGG + Chr5:1311911-1311930 None:intergenic 60.0%
GTGGCACTCCATGGACCTTC+AGG + Chr5:1312174-1312193 None:intergenic 60.0%
! TGTGTTGCCTGCGGTTTGGG+TGG - Chr5:1309729-1309748 MsG0580024144.01.T01:intron 60.0%
!! TGGATGGGTTGCGCTCGATG+AGG + Chr5:1312143-1312162 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 1309467 1313233 1309467 ID=MsG0580024144.01;Name=MsG0580024144.01
Chr5 mRNA 1309467 1313233 1309467 ID=MsG0580024144.01.T01;Parent=MsG0580024144.01;Name=MsG0580024144.01.T01;_AED=0.42;_eAED=0.55;_QI=0|0.2|0.16|1|0.4|0.33|6|156|205
Chr5 exon 1309467 1309660 1309467 ID=MsG0580024144.01.T01:exon:395;Parent=MsG0580024144.01.T01
Chr5 exon 1309757 1309845 1309757 ID=MsG0580024144.01.T01:exon:394;Parent=MsG0580024144.01.T01
Chr5 exon 1310387 1310649 1310387 ID=MsG0580024144.01.T01:exon:393;Parent=MsG0580024144.01.T01
Chr5 exon 1310755 1310850 1310755 ID=MsG0580024144.01.T01:exon:392;Parent=MsG0580024144.01.T01
Chr5 exon 1312959 1313041 1312959 ID=MsG0580024144.01.T01:exon:391;Parent=MsG0580024144.01.T01
Chr5 exon 1313185 1313233 1313185 ID=MsG0580024144.01.T01:exon:390;Parent=MsG0580024144.01.T01
Chr5 CDS 1313185 1313233 1313185 ID=MsG0580024144.01.T01:cds;Parent=MsG0580024144.01.T01
Chr5 CDS 1312959 1313041 1312959 ID=MsG0580024144.01.T01:cds;Parent=MsG0580024144.01.T01
Chr5 CDS 1310755 1310850 1310755 ID=MsG0580024144.01.T01:cds;Parent=MsG0580024144.01.T01
Chr5 CDS 1310387 1310649 1310387 ID=MsG0580024144.01.T01:cds;Parent=MsG0580024144.01.T01
Chr5 CDS 1309757 1309845 1309757 ID=MsG0580024144.01.T01:cds;Parent=MsG0580024144.01.T01
Chr5 CDS 1309623 1309660 1309623 ID=MsG0580024144.01.T01:cds;Parent=MsG0580024144.01.T01
Chr5 three_prime_UTR 1309467 1309622 1309467 ID=MsG0580024144.01.T01:three_prime_utr;Parent=MsG0580024144.01.T01
Gene Sequence

>MsG0580024144.01.T01

ATGCAGGTAATGGCTTGGAGGTATTTGCTAGCATCGAAGACTCCTCCTAAAGGGTCGGTCGACTATAGGCCTGTGCAAGCAGACTTACAACAAAAGGTTCCTAATTTTACTATAGTTTGTGTGTTGCCTGCGAAAGAGAAAAGTGAAGCTGAAATGGCAGCAGTGACTCTGAACCATATATCTCGAGAGTCCACCGATGTAAACCGCCTTGCCAAATTCTATGAAGAGATATTTGGATTTGAGGAAGTAGAAAGTCCCAAGTTCGGAGAGTTTAAGGTTGTATGGCTACGTCTTCCATCTTCTTCATTGTATCTTCACCTCATCGAGCGCAACCCATCCAACAATCTCCCTGAAGGTCCATGGAGTGCCACCTCTCCTGTTAAAGACCCTTCCCATCTTCCTAGAGGCCATCATCTCTGCTTCTCTGTCCCCAATTTCCAATCTTTCCTCCAAATTCTCAAGGTTTTCTTTTCATTTCATATGGTCCTAGGGATAAGGGCATTGAGACTTTTGAGAAATCTCTGCCTAATGGCAAAATCAAACAAGTTTTCTTCTTTGACCCTGATGGTAAGACTATGTAGTAATGGCTTGGAGGTTGCAAGCAAGGAAGACTCCTAG
Protein sequence

>MsG0580024144.01.T01

MQVMAWRYLLASKTPPKGSVDYRPVQADLQQKVPNFTIVCVLPAKEKSEAEMAAVTLNHISRESTDVNRLAKFYEEIFGFEEVESPKFGEFKVVWLRLPSSSLYLHLIERNPSNNLPEGPWSATSPVKDPSHLPRGHHLCFSVPNFQSFLQILKVFFSFHMVLGIRALRLLRNLCLMAKSNKFSSLTLMVRLCSNGLEVASKEDS*