AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024599.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024599.01.T01 MTR_3g112330 64.706 102 36 0 1 102 37 138 1.21e-44 143
MsG0580024599.01.T01 MTR_3g070320 71.111 90 26 0 10 99 1 90 1.97e-44 142
MsG0580024599.01.T01 MTR_6g072515 59.000 100 41 0 10 109 1 100 6.34e-40 130
MsG0580024599.01.T01 MTR_2g090750 62.500 88 33 0 1 88 599 686 6.03e-33 120
MsG0580024599.01.T01 MTR_5g007390 46.296 108 57 1 1 107 11 118 6.81e-32 108
MsG0580024599.01.T01 MTR_5g048020 54.945 91 41 0 1 91 83 173 3.57e-30 106
MsG0580024599.01.T01 MTR_5g027710 49.000 100 51 0 10 109 1 100 6.53e-30 103
MsG0580024599.01.T01 MTR_7g089465 56.790 81 35 0 18 98 21 101 3.28e-29 101
MsG0580024599.01.T01 MTR_4g093760 55.294 85 38 0 10 94 1 85 5.07e-27 98.6
MsG0580024599.01.T01 MTR_1g015073 46.875 96 51 0 1 96 23 118 4.38e-23 87.8
MsG0580024599.01.T01 MTR_8g077330 47.561 82 43 0 1 82 57 138 9.23e-22 85.1
MsG0580024599.01.T01 MTR_5g027610 42.424 99 57 0 10 108 1 99 1.43e-21 83.2
MsG0580024599.01.T01 MTR_4g459430 48.235 85 44 0 1 85 251 335 5.32e-21 85.9
MsG0580024599.01.T01 MTR_4g059530 48.235 85 44 0 1 85 251 335 5.32e-21 85.9
MsG0580024599.01.T01 MTR_2g090630 74.468 47 12 0 19 65 1 47 2.38e-20 79.0
MsG0580024599.01.T01 MTR_5g027090 80.952 42 8 0 68 109 14 55 7.79e-20 76.3
MsG0580024599.01.T01 MTR_7g106620 54.545 66 30 0 28 93 3 68 3.51e-18 74.3
MsG0580024599.01.T01 MTR_3g077360 50.877 57 28 0 53 109 1 57 1.20e-15 65.9
MsG0580024599.01.T01 MTR_1g100385 43.210 81 38 1 1 81 294 366 3.78e-15 70.1
MsG0580024599.01.T01 MTR_7g083290 56.604 53 23 0 57 109 142 194 2.02e-13 63.5
MsG0580024599.01.T01 MTR_8g074600 49.091 55 28 0 36 90 20 74 2.72e-13 61.6
MsG0580024599.01.T01 MTR_5g099210 51.923 52 25 0 58 109 18 69 3.16e-13 62.0
MsG0580024599.01.T01 MTR_4g037265 50.000 58 29 0 23 80 1 58 4.35e-13 62.4
MsG0580024599.01.T01 MTR_5g036810 49.153 59 30 0 1 59 237 295 5.22e-13 63.5
MsG0580024599.01.T01 MTR_8g446700 52.174 46 22 0 62 107 1 46 8.73e-13 59.7
MsG0580024599.01.T01 MTR_3g021160 51.064 47 23 0 63 109 3 49 1.96e-11 56.2
MsG0580024599.01.T01 MTR_3g108670 43.750 80 44 1 29 107 231 310 2.44e-11 58.9
MsG0580024599.01.T01 MTR_1g052925 66.667 39 13 0 45 83 1 39 4.33e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGATATGGCTAGAAATATT+TGG 0.242600 5:+6750918 None:intergenic
TCCCAATGGATAAACAAATT+TGG 0.244061 5:-6750983 None:intergenic
AAATATTTGGAGGAGTTTAA+TGG 0.268440 5:+6750931 MsG0580024599.01.T01:CDS
AAGTGGCGATTTGGGTAATT+TGG 0.301231 5:+6751065 MsG0580024599.01.T01:CDS
TGATTTGGCAAGTGGCGATT+TGG 0.313370 5:+6751056 MsG0580024599.01.T01:CDS
GTTGTCTTGGAGGTGGGTGT+TGG 0.316052 5:+6751159 MsG0580024599.01.T01:CDS
TCCAAATTTGTTTATCCATT+GGG 0.331325 5:+6750982 MsG0580024599.01.T01:CDS
GGAAGGGATTGCGGTTGATT+TGG 0.335809 5:+6751041 MsG0580024599.01.T01:CDS
AATATTTGGAGGAGTTTAAT+GGG 0.336070 5:+6750932 MsG0580024599.01.T01:CDS
TGAGATGGTGGAGGAGGTTA+AGG 0.340749 5:+6751135 MsG0580024599.01.T01:CDS
TTGTCTTGGAGGTGGGTGTT+GGG 0.344132 5:+6751160 MsG0580024599.01.T01:CDS
GTTTATCCATTGGGAGTGTT+GGG 0.364864 5:+6750991 MsG0580024599.01.T01:CDS
GTTCTTTAAATAAGAAAATT+CGG 0.376835 5:+6751020 MsG0580024599.01.T01:CDS
GATTTGGCAAGTGGCGATTT+GGG 0.380918 5:+6751057 MsG0580024599.01.T01:CDS
CTCCAAATTTGTTTATCCAT+TGG 0.393697 5:+6750981 MsG0580024599.01.T01:CDS
TGTTTATCCATTGGGAGTGT+TGG 0.416528 5:+6750990 MsG0580024599.01.T01:CDS
TAAGGTGTTGTCTTGGAGGT+GGG 0.425154 5:+6751153 MsG0580024599.01.T01:CDS
TTAAGGTGTTGTCTTGGAGG+TGG 0.426272 5:+6751152 MsG0580024599.01.T01:CDS
AGGTTAAGGTGTTGTCTTGG+AGG 0.428623 5:+6751149 MsG0580024599.01.T01:CDS
AGGAGGTTAAGGTGTTGTCT+TGG 0.443903 5:+6751146 MsG0580024599.01.T01:CDS
ATCCATTGGGAGTGTTGGGA+GGG 0.463309 5:+6750995 MsG0580024599.01.T01:CDS
CATTGGGAGTGTTGGGAGGG+AGG 0.473373 5:+6750998 MsG0580024599.01.T01:CDS
CAATAATGAGCTTGCTAGGT+GGG 0.480034 5:+6751111 MsG0580024599.01.T01:CDS
GTGGGATGAGATGGTGGAGG+AGG 0.480102 5:+6751129 MsG0580024599.01.T01:CDS
TATCCATTGGGAGTGTTGGG+AGG 0.491156 5:+6750994 MsG0580024599.01.T01:CDS
GGCGATTTGGGTAATTTGGA+AGG 0.516684 5:+6751069 MsG0580024599.01.T01:CDS
TTTAAATAAGAAAATTCGGA+AGG 0.517798 5:+6751024 MsG0580024599.01.T01:CDS
CACTCGTAAAACATACAAGC+CGG 0.518524 5:-6751196 None:intergenic
CGAGTGGAGTTGGTGTCCGC+GGG 0.527420 5:+6751213 MsG0580024599.01.T01:CDS
CTCCCTCCCAACACTCCCAA+TGG 0.538796 5:-6750997 None:intergenic
AGAAAATTCGGAAGGGATTG+CGG 0.543830 5:+6751032 MsG0580024599.01.T01:CDS
CAATGGATAAACAAATTTGG+AGG 0.558332 5:-6750980 None:intergenic
ACGAGTGGAGTTGGTGTCCG+CGG 0.558716 5:+6751212 MsG0580024599.01.T01:CDS
TTTGGAGGAGTTTAATGGGG+TGG 0.562777 5:+6750936 MsG0580024599.01.T01:CDS
ATTGCGGTTGATTTGGCAAG+TGG 0.568446 5:+6751048 MsG0580024599.01.T01:CDS
ATATTTGGAGGAGTTTAATG+GGG 0.575031 5:+6750933 MsG0580024599.01.T01:CDS
TTAAATAAGAAAATTCGGAA+GGG 0.578307 5:+6751025 MsG0580024599.01.T01:CDS
TGCTAGGTGGGATGAGATGG+TGG 0.580942 5:+6751123 MsG0580024599.01.T01:CDS
GCTTGCTAGGTGGGATGAGA+TGG 0.593043 5:+6751120 MsG0580024599.01.T01:CDS
GTTGGGTAGATTTAATAACC+CGG 0.593410 5:+6751177 MsG0580024599.01.T01:CDS
GTAATTTGGAAGGCTAGAAA+CGG 0.611611 5:+6751079 MsG0580024599.01.T01:CDS
ACTCGTAAAACATACAAGCC+GGG 0.614655 5:-6751195 None:intergenic
TAGGTGGGATGAGATGGTGG+AGG 0.646079 5:+6751126 MsG0580024599.01.T01:CDS
ATATGGCTAGAAATATTTGG+AGG 0.651693 5:+6750921 None:intergenic
TCAATAATGAGCTTGCTAGG+TGG 0.675953 5:+6751110 MsG0580024599.01.T01:CDS
TATCTTATCAGACACTCCCG+CGG 0.717026 5:-6751229 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTCTTTAAATAAGAAAATT+CGG + Chr5:6751020-6751039 MsG0580024599.01.T01:CDS 15.0%
!! TTAAATAAGAAAATTCGGAA+GGG + Chr5:6751025-6751044 MsG0580024599.01.T01:CDS 20.0%
!! TTTAAATAAGAAAATTCGGA+AGG + Chr5:6751024-6751043 MsG0580024599.01.T01:CDS 20.0%
! AAATATTTGGAGGAGTTTAA+TGG + Chr5:6750931-6750950 MsG0580024599.01.T01:CDS 25.0%
! AATATTTGGAGGAGTTTAAT+GGG + Chr5:6750932-6750951 MsG0580024599.01.T01:CDS 25.0%
!! TCCAAATTTGTTTATCCATT+GGG + Chr5:6750982-6751001 MsG0580024599.01.T01:CDS 25.0%
ATATTTGGAGGAGTTTAATG+GGG + Chr5:6750933-6750952 MsG0580024599.01.T01:CDS 30.0%
CAATGGATAAACAAATTTGG+AGG - Chr5:6750983-6751002 None:intergenic 30.0%
TCCCAATGGATAAACAAATT+TGG - Chr5:6750986-6751005 None:intergenic 30.0%
! CTCCAAATTTGTTTATCCAT+TGG + Chr5:6750981-6751000 MsG0580024599.01.T01:CDS 30.0%
!! TTTTCAATAATGAGCTTGCT+AGG + Chr5:6751107-6751126 MsG0580024599.01.T01:CDS 30.0%
GTAATTTGGAAGGCTAGAAA+CGG + Chr5:6751079-6751098 MsG0580024599.01.T01:CDS 35.0%
GTTGGGTAGATTTAATAACC+CGG + Chr5:6751177-6751196 MsG0580024599.01.T01:CDS 35.0%
ACTCGTAAAACATACAAGCC+GGG - Chr5:6751198-6751217 None:intergenic 40.0%
AGAAAATTCGGAAGGGATTG+CGG + Chr5:6751032-6751051 MsG0580024599.01.T01:CDS 40.0%
CACTCGTAAAACATACAAGC+CGG - Chr5:6751199-6751218 None:intergenic 40.0%
! AAGTGGCGATTTGGGTAATT+TGG + Chr5:6751065-6751084 MsG0580024599.01.T01:CDS 40.0%
! GTATGTTTTACGAGTGGAGT+TGG + Chr5:6751203-6751222 MsG0580024599.01.T01:CDS 40.0%
! TGTTTATCCATTGGGAGTGT+TGG + Chr5:6750990-6751009 MsG0580024599.01.T01:CDS 40.0%
!! CAATAATGAGCTTGCTAGGT+GGG + Chr5:6751111-6751130 MsG0580024599.01.T01:CDS 40.0%
!! GTTTATCCATTGGGAGTGTT+GGG + Chr5:6750991-6751010 MsG0580024599.01.T01:CDS 40.0%
!! TCAATAATGAGCTTGCTAGG+TGG + Chr5:6751110-6751129 MsG0580024599.01.T01:CDS 40.0%
GATTTGGCAAGTGGCGATTT+GGG + Chr5:6751057-6751076 MsG0580024599.01.T01:CDS 45.0%
TATCTTATCAGACACTCCCG+CGG - Chr5:6751232-6751251 None:intergenic 45.0%
TTTGGAGGAGTTTAATGGGG+TGG + Chr5:6750936-6750955 MsG0580024599.01.T01:CDS 45.0%
! CGGCTTGTATGTTTTACGAG+TGG + Chr5:6751197-6751216 MsG0580024599.01.T01:CDS 45.0%
! GGCGATTTGGGTAATTTGGA+AGG + Chr5:6751069-6751088 MsG0580024599.01.T01:CDS 45.0%
! TGATTTGGCAAGTGGCGATT+TGG + Chr5:6751056-6751075 MsG0580024599.01.T01:CDS 45.0%
!! AGGAGGTTAAGGTGTTGTCT+TGG + Chr5:6751146-6751165 MsG0580024599.01.T01:CDS 45.0%
!! AGGTTAAGGTGTTGTCTTGG+AGG + Chr5:6751149-6751168 MsG0580024599.01.T01:CDS 45.0%
!! ATTGCGGTTGATTTGGCAAG+TGG + Chr5:6751048-6751067 MsG0580024599.01.T01:CDS 45.0%
!! TAAGGTGTTGTCTTGGAGGT+GGG + Chr5:6751153-6751172 MsG0580024599.01.T01:CDS 45.0%
!! TTAAGGTGTTGTCTTGGAGG+TGG + Chr5:6751152-6751171 MsG0580024599.01.T01:CDS 45.0%
GGAAGGGATTGCGGTTGATT+TGG + Chr5:6751041-6751060 MsG0580024599.01.T01:CDS 50.0%
TTGTCTTGGAGGTGGGTGTT+GGG + Chr5:6751160-6751179 MsG0580024599.01.T01:CDS 50.0%
!! ATCCATTGGGAGTGTTGGGA+GGG + Chr5:6750995-6751014 MsG0580024599.01.T01:CDS 50.0%
!! TATCCATTGGGAGTGTTGGG+AGG + Chr5:6750994-6751013 MsG0580024599.01.T01:CDS 50.0%
!! TGAGATGGTGGAGGAGGTTA+AGG + Chr5:6751135-6751154 MsG0580024599.01.T01:CDS 50.0%
GTTGTCTTGGAGGTGGGTGT+TGG + Chr5:6751159-6751178 MsG0580024599.01.T01:CDS 55.0%
TGCTAGGTGGGATGAGATGG+TGG + Chr5:6751123-6751142 MsG0580024599.01.T01:CDS 55.0%
! GCTTGCTAGGTGGGATGAGA+TGG + Chr5:6751120-6751139 MsG0580024599.01.T01:CDS 55.0%
! TAGGTGGGATGAGATGGTGG+AGG + Chr5:6751126-6751145 MsG0580024599.01.T01:CDS 55.0%
CTCCCTCCCAACACTCCCAA+TGG - Chr5:6751000-6751019 None:intergenic 60.0%
!! ACGAGTGGAGTTGGTGTCCG+CGG + Chr5:6751212-6751231 MsG0580024599.01.T01:CDS 60.0%
!! CATTGGGAGTGTTGGGAGGG+AGG + Chr5:6750998-6751017 MsG0580024599.01.T01:CDS 60.0%
!! GTGGGATGAGATGGTGGAGG+AGG + Chr5:6751129-6751148 MsG0580024599.01.T01:CDS 60.0%
!! CGAGTGGAGTTGGTGTCCGC+GGG + Chr5:6751213-6751232 MsG0580024599.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 6750923 6751252 6750923 ID=MsG0580024599.01;Name=MsG0580024599.01
Chr5 mRNA 6750923 6751252 6750923 ID=MsG0580024599.01.T01;Parent=MsG0580024599.01;Name=MsG0580024599.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|109
Chr5 exon 6750923 6751252 6750923 ID=MsG0580024599.01.T01:exon:859;Parent=MsG0580024599.01.T01
Chr5 CDS 6750923 6751252 6750923 ID=MsG0580024599.01.T01:cds;Parent=MsG0580024599.01.T01
Gene Sequence

>MsG0580024599.01.T01

ATGGCTAGAAATATTTGGAGGAGTTTAATGGGGTGGTTAGATATGAATTTTTTAATGCCTCCAAATTTGTTTATCCATTGGGAGTGTTGGGAGGGAGGTTCTTTAAATAAGAAAATTCGGAAGGGATTGCGGTTGATTTGGCAAGTGGCGATTTGGGTAATTTGGAAGGCTAGAAACGGTGTTATTTTCAATAATGAGCTTGCTAGGTGGGATGAGATGGTGGAGGAGGTTAAGGTGTTGTCTTGGAGGTGGGTGTTGGGTAGATTTAATAACCCGGCTTGTATGTTTTACGAGTGGAGTTGGTGTCCGCGGGAGTGTCTGATAAGATAA

Protein sequence

>MsG0580024599.01.T01

MARNIWRSLMGWLDMNFLMPPNLFIHWECWEGGSLNKKIRKGLRLIWQVAIWVIWKARNGVIFNNELARWDEMVEEVKVLSWRWVLGRFNNPACMFYEWSWCPRECLIR*