Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026031.01.T01 | PNX79928.1 | 68.293 | 82 | 26 | 0 | 1 | 82 | 11 | 92 | 1.76E-29 | 111 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026031.01.T01 | O04492 | 57.317 | 82 | 35 | 0 | 1 | 82 | 15 | 96 | 4.57E-23 | 94.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026031.01.T01 | A0A072V205 | 70.732 | 82 | 24 | 0 | 1 | 82 | 16 | 97 | 7.31e-29 | 119 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000097.01 | MsG0580026031.01 | -0.823288 | 1.501769e-53 | 1.287556e-50 |
| MsG0180000942.01 | MsG0580026031.01 | 0.807889 | 4.053523e-50 | 2.284356e-47 |
| MsG0180000977.01 | MsG0580026031.01 | 0.835487 | 1.630575e-56 | 1.996933e-53 |
| MsG0180001119.01 | MsG0580026031.01 | 0.804914 | 1.718712e-49 | 8.971968e-47 |
| MsG0180001120.01 | MsG0580026031.01 | 0.800844 | 1.191061e-48 | 5.603444e-46 |
| MsG0180004025.01 | MsG0580026031.01 | 0.808748 | 2.659449e-50 | 1.532925e-47 |
| MsG0180004106.01 | MsG0580026031.01 | 0.822485 | 2.309548e-53 | 1.935730e-50 |
| MsG0180004112.01 | MsG0580026031.01 | 0.834179 | 3.481300e-56 | 4.098877e-53 |
| MsG0180005339.01 | MsG0580026031.01 | 0.803122 | 4.052845e-49 | 2.020120e-46 |
| MsG0180005639.01 | MsG0580026031.01 | 0.822233 | 2.643432e-53 | 2.199649e-50 |
| MsG0180006054.01 | MsG0580026031.01 | 0.823928 | 1.063286e-53 | 9.283226e-51 |
| MsG0480019384.01 | MsG0580026031.01 | 0.821544 | 3.816212e-53 | 3.113999e-50 |
| MsG0480020322.01 | MsG0580026031.01 | 0.841323 | 5.093499e-58 | 7.456690e-55 |
| MsG0480020323.01 | MsG0580026031.01 | 0.848992 | 4.301966e-60 | 8.038776e-57 |
| MsG0480020433.01 | MsG0580026031.01 | 0.810466 | 1.136729e-50 | 6.857182e-48 |
| MsG0480021564.01 | MsG0580026031.01 | 0.800416 | 1.456487e-48 | 6.778146e-46 |
| MsG0480021690.01 | MsG0580026031.01 | 0.805806 | 1.117378e-49 | 5.967823e-47 |
| MsG0480022535.01 | MsG0580026031.01 | 0.867401 | 1.406728e-65 | 4.905653e-62 |
| MsG0480022572.01 | MsG0580026031.01 | 0.803051 | 4.192057e-49 | 2.085708e-46 |
| MsG0480022918.01 | MsG0580026031.01 | 0.807240 | 5.568225e-50 | 3.085482e-47 |
| MsG0580024447.01 | MsG0580026031.01 | 0.803581 | 3.256587e-49 | 1.642624e-46 |
| MsG0580024599.01 | MsG0580026031.01 | 0.810944 | 8.960391e-51 | 5.475165e-48 |
| MsG0580025226.01 | MsG0580026031.01 | 0.822500 | 2.291823e-53 | 1.921703e-50 |
| MsG0580025644.01 | MsG0580026031.01 | 0.834440 | 2.994305e-56 | 3.553716e-53 |
| MsG0580025827.01 | MsG0580026031.01 | 0.806164 | 9.397373e-50 | 5.065258e-47 |
| MsG0580026031.01 | MsG0580026498.01 | 0.802193 | 6.302215e-49 | 3.067811e-46 |
| MsG0580026031.01 | MsG0580027789.01 | 0.814855 | 1.245624e-51 | 8.452376e-49 |
| MsG0580026031.01 | MsG0580028177.01 | 0.804670 | 1.932539e-49 | 1.002407e-46 |
| MsG0580026031.01 | MsG0580028179.01 | 0.831835 | 1.332792e-55 | 1.463577e-52 |
| MsG0580026031.01 | MsG0680031996.01 | 0.829020 | 6.503321e-55 | 6.574083e-52 |
| MsG0580026031.01 | MsG0680032286.01 | 0.824027 | 1.007986e-53 | 8.824176e-51 |
| MsG0580026031.01 | MsG0680032310.01 | 0.801773 | 7.685202e-49 | 3.701348e-46 |
| MsG0580026031.01 | MsG0680033136.01 | 0.822343 | 2.492615e-53 | 2.080737e-50 |
| MsG0580026031.01 | MsG0780036509.01 | 0.812795 | 3.542718e-51 | 2.273586e-48 |
| MsG0580026031.01 | MsG0780037105.01 | 0.847387 | 1.194612e-59 | 2.119885e-56 |
| MsG0580026031.01 | MsG0780039336.01 | 0.808805 | 2.585077e-50 | 1.492292e-47 |
| MsG0580026031.01 | MsG0780039438.01 | 0.805492 | 1.300447e-49 | 6.889603e-47 |
| MsG0580026031.01 | MsG0880041852.01 | 0.808009 | 3.822426e-50 | 2.161022e-47 |
| MsG0580026031.01 | MsG0880042000.01 | 0.805228 | 1.477125e-49 | 7.772588e-47 |
| MsG0580026031.01 | MsG0880042085.01 | 0.809845 | 1.547294e-50 | 9.183069e-48 |
| MsG0580026031.01 | MsG0880042086.01 | 0.811320 | 7.427716e-51 | 4.583273e-48 |
| MsG0580026031.01 | MsG0880042416.01 | 0.813375 | 2.642547e-51 | 1.722816e-48 |
| MsG0580026031.01 | MsG0880042948.01 | 0.833795 | 4.344165e-56 | 5.056939e-53 |
| MsG0580026031.01 | MsG0880044615.01 | 0.823112 | 1.650109e-53 | 1.407880e-50 |
| MsG0580026031.01 | MsG0880044619.01 | 0.808387 | 3.175978e-50 | 1.813586e-47 |
| MsG0580026031.01 | MsG0880044620.01 | 0.802107 | 6.564186e-49 | 3.188403e-46 |
| MsG0580026031.01 | MsG0880044625.01 | 0.806928 | 6.483314e-50 | 3.563473e-47 |
| MsG0580026031.01 | MsG0880044629.01 | 0.816233 | 6.147205e-52 | 4.331369e-49 |
| MsG0580026031.01 | MsG0880044876.01 | 0.802647 | 5.079177e-49 | 2.501284e-46 |
| MsG0580026031.01 | MsG0880045771.01 | 0.815335 | 9.745824e-52 | 6.700355e-49 |
| MsG0580026031.01 | MsG0880045992.01 | 0.805135 | 1.544963e-49 | 8.110443e-47 |
| MsG0580026031.01 | MsG0880046207.01 | 0.817046 | 4.039748e-52 | 2.910644e-49 |
| MsG0580026031.01 | MsG0880047206.01 | 0.817296 | 3.548608e-52 | 2.574210e-49 |
| MsG0580026031.01 | MsG0880047481.01 | -0.805177 | 1.513689e-49 | 7.954819e-47 |
| MsG0280006552.01 | MsG0580026031.01 | 0.836209 | 1.070156e-56 | 1.339605e-53 |
| MsG0280007867.01 | MsG0580026031.01 | 0.806748 | 7.074150e-50 | 3.870274e-47 |
| MsG0280009757.01 | MsG0580026031.01 | 0.803439 | 3.484940e-49 | 1.751278e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026031.01.T01 | MTR_3g100120 | 70.732 | 82 | 24 | 0 | 1 | 82 | 16 | 97 | 1.85e-32 | 119 |
| MsG0580026031.01.T01 | MTR_3g100120 | 70.732 | 82 | 24 | 0 | 1 | 82 | 18 | 99 | 1.99e-32 | 118 |
| MsG0580026031.01.T01 | MTR_7g114310 | 41.667 | 84 | 48 | 1 | 1 | 84 | 1 | 83 | 1.18e-11 | 59.7 |
| MsG0580026031.01.T01 | MTR_2g094490 | 44.928 | 69 | 38 | 0 | 1 | 69 | 1 | 69 | 3.99e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026031.01.T01 | AT1G09700 | 57.317 | 82 | 35 | 0 | 1 | 82 | 15 | 96 | 1.09e-24 | 94.4 |
| MsG0580026031.01.T01 | AT1G09700 | 57.317 | 82 | 35 | 0 | 1 | 82 | 15 | 96 | 4.66e-24 | 94.4 |
| MsG0580026031.01.T01 | AT3G26932 | 42.857 | 84 | 47 | 1 | 1 | 84 | 1 | 83 | 1.60e-13 | 64.7 |
| MsG0580026031.01.T01 | AT3G26932 | 42.857 | 84 | 47 | 1 | 1 | 84 | 1 | 83 | 1.60e-13 | 64.7 |
| MsG0580026031.01.T01 | AT2G28380 | 44.928 | 69 | 38 | 0 | 1 | 69 | 1 | 69 | 4.38e-11 | 58.2 |
Find 16 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGACCTGAAAGAAAGTTTA+TGG | 0.183803 | 5:-29440875 | None:intergenic |
| CTCTCCATAAACTTTCTTTC+AGG | 0.227895 | 5:+29440871 | MsG0580026031.01.T01:CDS |
| CTGATTAACATCACTAGTTT+TGG | 0.290642 | 5:-29440997 | None:intergenic |
| AGCTGCAAAGGTTGCTTTGA+TGG | 0.320686 | 5:+29440968 | MsG0580026031.01.T01:CDS |
| TGCGCTAAGGCTCCTCTTAT+AGG | 0.350426 | 5:-29441059 | None:intergenic |
| GCGCTAAGGCTCCTCTTATA+GGG | 0.413596 | 5:-29441058 | None:intergenic |
| AGCAGAGCAGTCAGCTGCAA+AGG | 0.457336 | 5:+29440956 | MsG0580026031.01.T01:CDS |
| TCTGTGCATACTCTTGCAAT+CGG | 0.492017 | 5:-29440803 | None:intergenic |
| AAAGGTTGCTTTGATGGAGT+TGG | 0.510442 | 5:+29440974 | MsG0580026031.01.T01:CDS |
| TCAACTTTATTGTTGCGCTA+AGG | 0.545537 | 5:-29441072 | None:intergenic |
| ATCTACAACAAGTTGAGTGA+TGG | 0.564808 | 5:-29441021 | None:intergenic |
| GTGTATACAACTACTAGTGA+AGG | 0.572322 | 5:+29440846 | MsG0580026031.01.T01:CDS |
| TGTATACAACTACTAGTGAA+GGG | 0.645234 | 5:+29440847 | MsG0580026031.01.T01:CDS |
| TCACTAGTAGTTGTATACAC+TGG | 0.652691 | 5:-29440843 | None:intergenic |
| CTGAAAGAAAGTTTATGGAG+AGG | 0.658722 | 5:-29440870 | None:intergenic |
| AGTAGTTGTATACACTGGTG+CGG | 0.710579 | 5:-29440838 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTTTCTCCCTATAAG+AGG | + | Chr5:29441047-29441066 | MsG0580026031.01.T01:CDS | 25.0% |
| TGTATACAACTACTAGTGAA+GGG | + | Chr5:29440847-29440866 | MsG0580026031.01.T01:CDS | 30.0% | |
| TTGACCTGAAAGAAAGTTTA+TGG | - | Chr5:29440878-29440897 | None:intergenic | 30.0% | |
| !! | CTGATTAACATCACTAGTTT+TGG | - | Chr5:29441000-29441019 | None:intergenic | 30.0% |
| !! | GACTGAATTTTCTAAGAAGA+AGG | + | Chr5:29440932-29440951 | MsG0580026031.01.T01:CDS | 30.0% |
| CTCTCCATAAACTTTCTTTC+AGG | + | Chr5:29440871-29440890 | MsG0580026031.01.T01:CDS | 35.0% | |
| CTGAAAGAAAGTTTATGGAG+AGG | - | Chr5:29440873-29440892 | None:intergenic | 35.0% | |
| GTGTATACAACTACTAGTGA+AGG | + | Chr5:29440846-29440865 | MsG0580026031.01.T01:CDS | 35.0% | |
| TCACTAGTAGTTGTATACAC+TGG | - | Chr5:29440846-29440865 | None:intergenic | 35.0% | |
| ! | ATCTACAACAAGTTGAGTGA+TGG | - | Chr5:29441024-29441043 | None:intergenic | 35.0% |
| AGTAGTTGTATACACTGGTG+CGG | - | Chr5:29440841-29440860 | None:intergenic | 40.0% | |
| TCTGTGCATACTCTTGCAAT+CGG | - | Chr5:29440806-29440825 | None:intergenic | 40.0% | |
| ! | AAAGGTTGCTTTGATGGAGT+TGG | + | Chr5:29440974-29440993 | MsG0580026031.01.T01:CDS | 40.0% |
| ! | AGCTGCAAAGGTTGCTTTGA+TGG | + | Chr5:29440968-29440987 | MsG0580026031.01.T01:CDS | 45.0% |
| GCGCTAAGGCTCCTCTTATA+GGG | - | Chr5:29441061-29441080 | None:intergenic | 50.0% | |
| TGCGCTAAGGCTCCTCTTAT+AGG | - | Chr5:29441062-29441081 | None:intergenic | 50.0% | |
| AGCAGAGCAGTCAGCTGCAA+AGG | + | Chr5:29440956-29440975 | MsG0580026031.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 29440792 | 29441088 | 29440792 | ID=MsG0580026031.01;Name=MsG0580026031.01 |
| Chr5 | mRNA | 29440792 | 29441088 | 29440792 | ID=MsG0580026031.01.T01;Parent=MsG0580026031.01;Name=MsG0580026031.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|98 |
| Chr5 | exon | 29440792 | 29441088 | 29440792 | ID=MsG0580026031.01.T01:exon:33513;Parent=MsG0580026031.01.T01 |
| Chr5 | CDS | 29440792 | 29441088 | 29440792 | ID=MsG0580026031.01.T01:cds;Parent=MsG0580026031.01.T01 |
| Gene Sequence |
| Protein sequence |
