Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025546.01.T01 | XP_003612893.1 | 96.947 | 131 | 4 | 0 | 66 | 196 | 101 | 231 | 1.67E-82 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025546.01.T01 | G7KDT4 | 96.947 | 131 | 4 | 0 | 66 | 196 | 101 | 231 | 7.98e-83 | 254 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048210.01 | MsG0580025546.01 | 0.801627 | 8.236839e-49 | 3.952216e-46 |
| MsG0180000797.01 | MsG0580025546.01 | 0.802880 | 4.547821e-49 | 2.252956e-46 |
| MsG0180001922.01 | MsG0580025546.01 | 0.807018 | 6.202472e-50 | 3.417193e-47 |
| MsG0280011235.01 | MsG0580025546.01 | 0.814784 | 1.291317e-51 | 8.745829e-49 |
| MsG0480020487.01 | MsG0580025546.01 | 0.828048 | 1.116212e-54 | 1.096969e-51 |
| MsG0580025453.01 | MsG0580025546.01 | 0.804673 | 1.929425e-49 | 1.000871e-46 |
| MsG0580025520.01 | MsG0580025546.01 | 0.845920 | 3.004796e-59 | 5.086270e-56 |
| MsG0580025546.01 | MsG0580025769.01 | 0.815050 | 1.127539e-51 | 7.691422e-49 |
| MsG0580025546.01 | MsG0780040389.01 | 0.808935 | 2.425550e-50 | 1.405012e-47 |
| MsG0580025546.01 | MsG0780040679.01 | 0.806397 | 8.391504e-50 | 4.549771e-47 |
| MsG0580025546.01 | MsG0780040758.01 | 0.803852 | 2.860134e-49 | 1.452708e-46 |
| MsG0580025546.01 | MsG0880042223.01 | 0.807572 | 4.734228e-50 | 2.645988e-47 |
| MsG0580025546.01 | MsG0880045106.01 | 0.806817 | 6.840639e-50 | 3.749323e-47 |
| MsG0580025546.01 | MsG0880046244.01 | 0.820683 | 6.025752e-53 | 4.800032e-50 |
| MsG0380017158.01 | MsG0580025546.01 | 0.800588 | 1.343136e-48 | 6.277905e-46 |
| MsG0280006935.01 | MsG0580025546.01 | 0.801130 | 1.041160e-48 | 4.933919e-46 |
| MsG0280007549.01 | MsG0580025546.01 | 0.804791 | 1.823089e-49 | 9.486375e-47 |
| MsG0280008295.01 | MsG0580025546.01 | 0.808658 | 2.780163e-50 | 1.598699e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025546.01.T01 | MTR_5g030210 | 96.947 | 131 | 4 | 0 | 66 | 196 | 101 | 231 | 2.02e-86 | 254 |
| MsG0580025546.01.T01 | MTR_5g430370 | 96.947 | 131 | 4 | 0 | 66 | 196 | 101 | 231 | 2.02e-86 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025546.01.T01 | AT1G32690 | 43.243 | 111 | 25 | 5 | 92 | 196 | 122 | 200 | 9.94e-14 | 67.0 |
| MsG0580025546.01.T01 | AT2G35200 | 54.688 | 64 | 21 | 3 | 135 | 196 | 144 | 201 | 1.58e-11 | 61.2 |
Find 62 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCTGTGACGGCGGTTTC+TGG | 0.145330 | 5:+20279832 | MsG0580025546.01.T01:CDS |
| CTTTAGAAGCTTTCGATTGC+CGG | 0.319927 | 5:-20279930 | None:intergenic |
| GCTACGACGGTCTCGGTCTA+TGG | 0.334307 | 5:+20279556 | MsG0580025546.01.T01:CDS |
| GAAAGCTTCTAAAGTTGTTC+AGG | 0.349932 | 5:+20279939 | MsG0580025546.01.T01:CDS |
| GCAAGTCAGCGAGGTCGTTT+TGG | 0.364935 | 5:+20279671 | MsG0580025546.01.T01:CDS |
| AGATGAAGTTGAAGCGTAAC+AGG | 0.369906 | 5:-20279436 | None:intergenic |
| AGAGATTCTCCGGCGATTAA+CGG | 0.402315 | 5:+20279646 | MsG0580025546.01.T01:CDS |
| GAGTAGAAAAGCGGCGGTTA+TGG | 0.406718 | 5:+20279795 | MsG0580025546.01.T01:CDS |
| GGAGTGAATCGGAGCGAGAT+TGG | 0.419488 | 5:+20279731 | MsG0580025546.01.T01:CDS |
| GAACGTTCTCCTTTGTATAG+AGG | 0.436369 | 5:+20279961 | MsG0580025546.01.T01:CDS |
| TCCGTCACCGGCGACGGCGT+TGG | 0.448295 | 5:+20279492 | MsG0580025546.01.T01:CDS |
| GAGTGAATCGGAGCGAGATT+GGG | 0.455212 | 5:+20279732 | MsG0580025546.01.T01:CDS |
| TGCCGGCGGAGGTAAAGAAT+TGG | 0.461758 | 5:+20279615 | MsG0580025546.01.T01:CDS |
| TTCTTTACCTCCGCCGGCAC+CGG | 0.464419 | 5:-20279611 | None:intergenic |
| GTCAAGGTCGGTGGCTGTGA+CGG | 0.472779 | 5:+20279822 | MsG0580025546.01.T01:CDS |
| CGGAAAACCTTCATCGGACT+CGG | 0.474549 | 5:-20279910 | None:intergenic |
| TCTCGATCGCGATCGAGAAA+CGG | 0.477030 | 5:-20279584 | None:intergenic |
| GGAGATGAAGAAGGTGTTGA+CGG | 0.481055 | 5:+20279753 | MsG0580025546.01.T01:CDS |
| TTCAACTTCATCTTCTTCAT+CGG | 0.483444 | 5:+20279446 | MsG0580025546.01.T01:CDS |
| AAGGTCGGTGGCTGTGACGG+CGG | 0.490130 | 5:+20279825 | MsG0580025546.01.T01:CDS |
| ATCGCCGGAGAATCTCTATC+AGG | 0.491493 | 5:-20279640 | None:intergenic |
| AGATTCTCCGGTGCCGGCGG+AGG | 0.496962 | 5:+20279604 | MsG0580025546.01.T01:CDS |
| CGATCGAGATTCTCCGGTGC+CGG | 0.502818 | 5:+20279598 | MsG0580025546.01.T01:CDS |
| TAGAGCGCCGCCGCGTTCAA+AGG | 0.512903 | 5:+20279870 | MsG0580025546.01.T01:CDS |
| GGGCGCACGTTAGCGCAGTC+AGG | 0.513482 | 5:-20279408 | None:intergenic |
| AGAGCGCCGCCGCGTTCAAA+GGG | 0.515549 | 5:+20279871 | MsG0580025546.01.T01:CDS |
| GTTCTTTCCGAGTCCGATGA+AGG | 0.518375 | 5:+20279903 | MsG0580025546.01.T01:CDS |
| TTCCAATTCTTTACCTCCGC+CGG | 0.519879 | 5:-20279617 | None:intergenic |
| ACGGCGGTTTCTGGCGGTGA+CGG | 0.521255 | 5:+20279841 | MsG0580025546.01.T01:CDS |
| AACCGCTCCAACGCCGTCGC+CGG | 0.524687 | 5:-20279499 | None:intergenic |
| CATAAGAGTAACAGTAACAG+AGG | 0.528054 | 5:+20279709 | MsG0580025546.01.T01:CDS |
| TCCAACGCCGTCGCCGGTGA+CGG | 0.528777 | 5:-20279493 | None:intergenic |
| GGAACCTGATAGAGATTCTC+CGG | 0.530069 | 5:+20279636 | MsG0580025546.01.T01:CDS |
| GATTGCCGGAAAACCTTCAT+CGG | 0.534304 | 5:-20279916 | None:intergenic |
| CCGCCGCGTTCAAAGGGAAA+GGG | 0.536223 | 5:+20279877 | MsG0580025546.01.T01:CDS |
| GAACTTCGCTACGACGGTCT+CGG | 0.545347 | 5:+20279549 | MsG0580025546.01.T01:CDS |
| GGCGACGGCGTTGGAGCGGT+TGG | 0.553785 | 5:+20279501 | MsG0580025546.01.T01:CDS |
| CACCGGCGACGGCGTTGGAG+CGG | 0.557404 | 5:+20279497 | MsG0580025546.01.T01:CDS |
| CGATCGCGATCGAGATTCTC+CGG | 0.558476 | 5:+20279592 | MsG0580025546.01.T01:CDS |
| GCGAGATTGGGAGATGAAGA+AGG | 0.564675 | 5:+20279744 | MsG0580025546.01.T01:CDS |
| CCCTTTCCCTTTGAACGCGG+CGG | 0.565669 | 5:-20279877 | None:intergenic |
| GACTTGCTTCCGTTAATCGC+CGG | 0.567137 | 5:-20279655 | None:intergenic |
| CAGTAACAGAGGGAGTGAAT+CGG | 0.569768 | 5:+20279720 | MsG0580025546.01.T01:CDS |
| GCCGCCGCGTTCAAAGGGAA+AGG | 0.573012 | 5:+20279876 | MsG0580025546.01.T01:CDS |
| GAAAAGCGGCGGTTATGGCT+AGG | 0.579162 | 5:+20279800 | MsG0580025546.01.T01:CDS |
| CAATTTCAACCTCTATACAA+AGG | 0.592513 | 5:-20279970 | None:intergenic |
| GTTGAAGCGTAACAGGAACA+TGG | 0.592953 | 5:-20279429 | None:intergenic |
| GGTTATGGCTAGGTCAAGGT+CGG | 0.593743 | 5:+20279810 | MsG0580025546.01.T01:CDS |
| CATCCCTTTCCCTTTGAACG+CGG | 0.595896 | 5:-20279880 | None:intergenic |
| TCGAGATTCTCCGGTGCCGG+CGG | 0.600382 | 5:+20279601 | MsG0580025546.01.T01:CDS |
| GATGAAGAAGGTGTTGACGG+AGG | 0.604201 | 5:+20279756 | MsG0580025546.01.T01:CDS |
| GCTGTGACGGCGGTTTCTGG+CGG | 0.605816 | 5:+20279835 | MsG0580025546.01.T01:CDS |
| TATGGCTAGGTCAAGGTCGG+TGG | 0.608472 | 5:+20279813 | MsG0580025546.01.T01:CDS |
| CGGCGGTTATGGCTAGGTCA+AGG | 0.634980 | 5:+20279806 | MsG0580025546.01.T01:CDS |
| TTGAAGCGTAACAGGAACAT+GGG | 0.644311 | 5:-20279428 | None:intergenic |
| CGCGTTCAAAGGGAAAGGGA+TGG | 0.648433 | 5:+20279881 | MsG0580025546.01.T01:CDS |
| GATCGTGAACTTCGCTACGA+CGG | 0.650872 | 5:+20279543 | MsG0580025546.01.T01:CDS |
| CGGTGATGTGAGTAGAAAAG+CGG | 0.673609 | 5:+20279786 | MsG0580025546.01.T01:CDS |
| ATAAGAGTAACAGTAACAGA+GGG | 0.689356 | 5:+20279710 | MsG0580025546.01.T01:CDS |
| TGATGTGAGTAGAAAAGCGG+CGG | 0.691902 | 5:+20279789 | MsG0580025546.01.T01:CDS |
| GTGTTGACGGAGGAAATCGA+CGG | 0.751703 | 5:+20279766 | MsG0580025546.01.T01:CDS |
| TTAACGGAAGCAAGTCAGCG+AGG | 0.770062 | 5:+20279662 | MsG0580025546.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAGAGTAACAGTAACAGA+GGG | + | Chr5:20279710-20279729 | MsG0580025546.01.T01:CDS | 30.0% | |
| TTCAACTTCATCTTCTTCAT+CGG | + | Chr5:20279446-20279465 | MsG0580025546.01.T01:CDS | 30.0% | |
| CATAAGAGTAACAGTAACAG+AGG | + | Chr5:20279709-20279728 | MsG0580025546.01.T01:CDS | 35.0% | |
| !! | GAAAGCTTCTAAAGTTGTTC+AGG | + | Chr5:20279939-20279958 | MsG0580025546.01.T01:CDS | 35.0% |
| !! | GTCGTTTTGGTCAATTTTCA+AGG | + | Chr5:20279684-20279703 | MsG0580025546.01.T01:CDS | 35.0% |
| AGATGAAGTTGAAGCGTAAC+AGG | - | Chr5:20279439-20279458 | None:intergenic | 40.0% | |
| GAACGTTCTCCTTTGTATAG+AGG | + | Chr5:20279961-20279980 | MsG0580025546.01.T01:CDS | 40.0% | |
| TTGAAGCGTAACAGGAACAT+GGG | - | Chr5:20279431-20279450 | None:intergenic | 40.0% | |
| ! | CTTTAGAAGCTTTCGATTGC+CGG | - | Chr5:20279933-20279952 | None:intergenic | 40.0% |
| ! | TTTTCTCGATTTTCCGTCAC+CGG | + | Chr5:20279480-20279499 | MsG0580025546.01.T01:CDS | 40.0% |
| AGAGATTCTCCGGCGATTAA+CGG | + | Chr5:20279646-20279665 | MsG0580025546.01.T01:CDS | 45.0% | |
| CAGTAACAGAGGGAGTGAAT+CGG | + | Chr5:20279720-20279739 | MsG0580025546.01.T01:CDS | 45.0% | |
| CGGTGATGTGAGTAGAAAAG+CGG | + | Chr5:20279786-20279805 | MsG0580025546.01.T01:CDS | 45.0% | |
| GATTGCCGGAAAACCTTCAT+CGG | - | Chr5:20279919-20279938 | None:intergenic | 45.0% | |
| GGAACCTGATAGAGATTCTC+CGG | + | Chr5:20279636-20279655 | MsG0580025546.01.T01:CDS | 45.0% | |
| GTTGAAGCGTAACAGGAACA+TGG | - | Chr5:20279432-20279451 | None:intergenic | 45.0% | |
| TGATGTGAGTAGAAAAGCGG+CGG | + | Chr5:20279789-20279808 | MsG0580025546.01.T01:CDS | 45.0% | |
| TTCCAATTCTTTACCTCCGC+CGG | - | Chr5:20279620-20279639 | None:intergenic | 45.0% | |
| !! | GGAGATGAAGAAGGTGTTGA+CGG | + | Chr5:20279753-20279772 | MsG0580025546.01.T01:CDS | 45.0% |
| ATCGCCGGAGAATCTCTATC+AGG | - | Chr5:20279643-20279662 | None:intergenic | 50.0% | |
| CATCCCTTTCCCTTTGAACG+CGG | - | Chr5:20279883-20279902 | None:intergenic | 50.0% | |
| CGGAAAACCTTCATCGGACT+CGG | - | Chr5:20279913-20279932 | None:intergenic | 50.0% | |
| GAGTAGAAAAGCGGCGGTTA+TGG | + | Chr5:20279795-20279814 | MsG0580025546.01.T01:CDS | 50.0% | |
| GAGTGAATCGGAGCGAGATT+GGG | + | Chr5:20279732-20279751 | MsG0580025546.01.T01:CDS | 50.0% | |
| GATCGTGAACTTCGCTACGA+CGG | + | Chr5:20279543-20279562 | MsG0580025546.01.T01:CDS | 50.0% | |
| GCGAGATTGGGAGATGAAGA+AGG | + | Chr5:20279744-20279763 | MsG0580025546.01.T01:CDS | 50.0% | |
| GGTTATGGCTAGGTCAAGGT+CGG | + | Chr5:20279810-20279829 | MsG0580025546.01.T01:CDS | 50.0% | |
| GTTCTTTCCGAGTCCGATGA+AGG | + | Chr5:20279903-20279922 | MsG0580025546.01.T01:CDS | 50.0% | |
| TCTCGATCGCGATCGAGAAA+CGG | - | Chr5:20279587-20279606 | None:intergenic | 50.0% | |
| TTAACGGAAGCAAGTCAGCG+AGG | + | Chr5:20279662-20279681 | MsG0580025546.01.T01:CDS | 50.0% | |
| ! | CGAGTCCGATGAAGGTTTTC+CGG | + | Chr5:20279911-20279930 | MsG0580025546.01.T01:CDS | 50.0% |
| ! | GACTTGCTTCCGTTAATCGC+CGG | - | Chr5:20279658-20279677 | None:intergenic | 50.0% |
| ! | GTGTTGACGGAGGAAATCGA+CGG | + | Chr5:20279766-20279785 | MsG0580025546.01.T01:CDS | 50.0% |
| !! | GATGAAGAAGGTGTTGACGG+AGG | + | Chr5:20279756-20279775 | MsG0580025546.01.T01:CDS | 50.0% |
| CGATCGCGATCGAGATTCTC+CGG | + | Chr5:20279592-20279611 | MsG0580025546.01.T01:CDS | 55.0% | |
| CGCGTTCAAAGGGAAAGGGA+TGG | + | Chr5:20279881-20279900 | MsG0580025546.01.T01:CDS | 55.0% | |
| GAAAAGCGGCGGTTATGGCT+AGG | + | Chr5:20279800-20279819 | MsG0580025546.01.T01:CDS | 55.0% | |
| GAACTTCGCTACGACGGTCT+CGG | + | Chr5:20279549-20279568 | MsG0580025546.01.T01:CDS | 55.0% | |
| GGAGTGAATCGGAGCGAGAT+TGG | + | Chr5:20279731-20279750 | MsG0580025546.01.T01:CDS | 55.0% | |
| TATGGCTAGGTCAAGGTCGG+TGG | + | Chr5:20279813-20279832 | MsG0580025546.01.T01:CDS | 55.0% | |
| TGCCGGCGGAGGTAAAGAAT+TGG | + | Chr5:20279615-20279634 | MsG0580025546.01.T01:CDS | 55.0% | |
| ! | GCAAGTCAGCGAGGTCGTTT+TGG | + | Chr5:20279671-20279690 | MsG0580025546.01.T01:CDS | 55.0% |
| CCCTTTCCCTTTGAACGCGG+CGG | - | Chr5:20279880-20279899 | None:intergenic | 60.0% | |
| CCGCCGCGTTCAAAGGGAAA+GGG | + | Chr5:20279877-20279896 | MsG0580025546.01.T01:CDS | 60.0% | |
| CGATCGAGATTCTCCGGTGC+CGG | + | Chr5:20279598-20279617 | MsG0580025546.01.T01:CDS | 60.0% | |
| CGGCGGTTATGGCTAGGTCA+AGG | + | Chr5:20279806-20279825 | MsG0580025546.01.T01:CDS | 60.0% | |
| GCTACGACGGTCTCGGTCTA+TGG | + | Chr5:20279556-20279575 | MsG0580025546.01.T01:CDS | 60.0% | |
| GTCAAGGTCGGTGGCTGTGA+CGG | + | Chr5:20279822-20279841 | MsG0580025546.01.T01:CDS | 60.0% | |
| TTCTTTACCTCCGCCGGCAC+CGG | - | Chr5:20279614-20279633 | None:intergenic | 60.0% | |
| ! | CGATTTTCCGTCACCGGCGA+CGG | + | Chr5:20279486-20279505 | MsG0580025546.01.T01:CDS | 60.0% |
| AAGGTCGGTGGCTGTGACGG+CGG | + | Chr5:20279825-20279844 | MsG0580025546.01.T01:CDS | 65.0% | |
| GCCGCCGCGTTCAAAGGGAA+AGG | + | Chr5:20279876-20279895 | MsG0580025546.01.T01:CDS | 65.0% | |
| GTGGCTGTGACGGCGGTTTC+TGG | + | Chr5:20279832-20279851 | MsG0580025546.01.T01:CDS | 65.0% | |
| ! | GCTGTGACGGCGGTTTCTGG+CGG | + | Chr5:20279835-20279854 | MsG0580025546.01.T01:CDS | 65.0% |
| ! | TCGAGATTCTCCGGTGCCGG+CGG | + | Chr5:20279601-20279620 | MsG0580025546.01.T01:CDS | 65.0% |
| !! | ACGGCGGTTTCTGGCGGTGA+CGG | + | Chr5:20279841-20279860 | MsG0580025546.01.T01:CDS | 65.0% |
| !! | AGAGCGCCGCCGCGTTCAAA+GGG | + | Chr5:20279871-20279890 | MsG0580025546.01.T01:CDS | 65.0% |
| !! | TAGAGCGCCGCCGCGTTCAA+AGG | + | Chr5:20279870-20279889 | MsG0580025546.01.T01:CDS | 65.0% |
| AACCGCTCCAACGCCGTCGC+CGG | - | Chr5:20279502-20279521 | None:intergenic | 70.0% | |
| GGGCGCACGTTAGCGCAGTC+AGG | - | Chr5:20279411-20279430 | None:intergenic | 70.0% | |
| TCCAACGCCGTCGCCGGTGA+CGG | - | Chr5:20279496-20279515 | None:intergenic | 70.0% | |
| !! | AGATTCTCCGGTGCCGGCGG+AGG | + | Chr5:20279604-20279623 | MsG0580025546.01.T01:CDS | 70.0% |
| TCCGTCACCGGCGACGGCGT+TGG | + | Chr5:20279492-20279511 | MsG0580025546.01.T01:CDS | 75.0% | |
| ! | CACCGGCGACGGCGTTGGAG+CGG | + | Chr5:20279497-20279516 | MsG0580025546.01.T01:CDS | 75.0% |
| ! | GGCGACGGCGTTGGAGCGGT+TGG | + | Chr5:20279501-20279520 | MsG0580025546.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 20279397 | 20279987 | 20279397 | ID=MsG0580025546.01;Name=MsG0580025546.01 |
| Chr5 | mRNA | 20279397 | 20279987 | 20279397 | ID=MsG0580025546.01.T01;Parent=MsG0580025546.01;Name=MsG0580025546.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|196 |
| Chr5 | exon | 20279397 | 20279987 | 20279397 | ID=MsG0580025546.01.T01:exon:23253;Parent=MsG0580025546.01.T01 |
| Chr5 | CDS | 20279397 | 20279987 | 20279397 | ID=MsG0580025546.01.T01:cds;Parent=MsG0580025546.01.T01 |
| Gene Sequence |
| Protein sequence |
