AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011235.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011235.01.T01 MTR_2g100420 100.000 205 0 0 1 205 1 205 2.92e-150 415
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011235.01.T01 AT1G17730 87.805 205 23 1 1 205 1 203 1.09e-130 366
MsG0280011235.01.T01 AT1G73030 88.293 205 22 1 1 205 1 203 8.83e-119 335
MsG0280011235.01.T01 AT2G06530 27.273 165 116 2 19 180 7 170 6.62e-12 62.4
MsG0280011235.01.T01 AT2G06530 27.273 165 116 2 19 180 28 191 8.72e-12 62.4

Find 49 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGTTCAAGCGGAGTTTA+TGG 0.198154 2:-81300769 MsG0280011235.01.T01:CDS
CAACAGGTGGCGGATGATTA+TGG 0.251058 2:-81300684 MsG0280011235.01.T01:CDS
CGAGAAGCAGTTTGTGAATA+TGG 0.254306 2:-81300790 MsG0280011235.01.T01:CDS
GGGAAACATCGTGAAATCTT+TGG 0.350209 2:-81300868 MsG0280011235.01.T01:CDS
GGCGGCTGCTCATGCTGTTC+CGG 0.374454 2:-81300634 MsG0280011235.01.T01:CDS
TGCTCATGCTGTTCCGGTTA+AGG 0.374890 2:-81300628 MsG0280011235.01.T01:CDS
GGAAGTTTCGGTGAATCTTC+CGG 0.375555 2:-81300658 MsG0280011235.01.T01:CDS
TCAGCATCCTTCTCCTTAAC+CGG 0.398938 2:+81300615 None:intergenic
CATCCAATCGGGAGGATAAT+CGG 0.409666 2:+81300946 None:intergenic
TGATTATGGATTGGAAGTTT+CGG 0.427917 2:-81300670 MsG0280011235.01.T01:CDS
GGTTAATAGTTTGATGCAAC+AGG 0.440152 2:-81300700 MsG0280011235.01.T01:CDS
TTGGAATCGTCGTTGGCGAC+GGG 0.441222 2:-81300849 MsG0280011235.01.T01:CDS
TTCGGTGAATCTTCCGGTGG+CGG 0.464892 2:-81300652 MsG0280011235.01.T01:CDS
AAGCTTCTCTGTGTTTCCCA+TGG 0.476337 2:+81301154 None:intergenic
TAGTTTGATGCAACAGGTGG+CGG 0.477361 2:-81300694 MsG0280011235.01.T01:CDS
AAACTATTAACCTCCCCTTC+AGG 0.482512 2:+81300711 None:intergenic
GAGCACTCACGGCATCCAAT+CGG 0.483420 2:+81300934 None:intergenic
ACAGCATGAGCAGCCGCCAC+CGG 0.500440 2:+81300639 None:intergenic
AGCACTCACGGCATCCAATC+GGG 0.502128 2:+81300935 None:intergenic
CTTCGCTTTCGACTCCTGAA+GGG 0.513104 2:-81300725 MsG0280011235.01.T01:CDS
ACTTCGCTTTCGACTCCTGA+AGG 0.521847 2:-81300726 MsG0280011235.01.T01:CDS
GTCAACAATCAGCAAATCGA+TGG 0.522743 2:-81300889 MsG0280011235.01.T01:CDS
TGCTGTTCCGGTTAAGGAGA+AGG 0.523122 2:-81300622 MsG0280011235.01.T01:CDS
GAAGCTTCTGAATCAGATCA+TGG 0.534967 2:-81301138 MsG0280011235.01.T01:CDS
GAAATCTTTGGAATCGTCGT+TGG 0.542053 2:-81300856 MsG0280011235.01.T01:CDS
TTTGGAATCGTCGTTGGCGA+CGG 0.545316 2:-81300850 MsG0280011235.01.T01:CDS
TTCGCTTTCGACTCCTGAAG+GGG 0.555513 2:-81300724 MsG0280011235.01.T01:CDS
GCAGAAGATGTCGGAGACGA+TGG 0.561045 2:-81300820 MsG0280011235.01.T01:CDS
TAAGGAGAAGGATGCTGAGA+AGG 0.573843 2:-81300610 MsG0280011235.01.T01:CDS
GGAGTTTATGGAAAGTGCTA+TGG 0.582336 2:-81300757 MsG0280011235.01.T01:CDS
GGTGGCGGATGATTATGGAT+TGG 0.587630 2:-81300679 MsG0280011235.01.T01:CDS
CTTGCTGAGCTTAAAGCTAG+AGG 0.589782 2:-81300561 MsG0280011235.01.T01:CDS
CCTCCGATTATCCTCCCGAT+TGG 0.590956 2:-81300949 MsG0280011235.01.T01:CDS
GGATGCTGAGAAGGTTGATG+AGG 0.596267 2:-81300601 MsG0280011235.01.T01:CDS
GTTTATGGAAAGTGCTATGG+CGG 0.596862 2:-81300754 MsG0280011235.01.T01:CDS
TGTGAATATGGAGGTTCAAG+CGG 0.607620 2:-81300778 MsG0280011235.01.T01:CDS
TGTATCGAGACGAGCACTCA+CGG 0.607767 2:+81300923 None:intergenic
GCTTTCGACTCCTGAAGGGG+AGG 0.608667 2:-81300721 MsG0280011235.01.T01:CDS
TCAACAATCAGCAAATCGAT+GGG 0.616382 2:-81300888 MsG0280011235.01.T01:CDS
TGGAATCGTCGTTGGCGACG+GGG 0.616847 2:-81300848 MsG0280011235.01.T01:CDS
GGGGAATTTGCAGAAGATGT+CGG 0.623637 2:-81300829 MsG0280011235.01.T01:CDS
GAGTGCTCGTCTCGATACAC+AGG 0.631959 2:-81300919 MsG0280011235.01.T01:CDS
TTGATGAGGATGATTTGTCA+AGG 0.632184 2:-81300587 MsG0280011235.01.T01:CDS
TTTATGGAAAGTGCTATGGC+GGG 0.633565 2:-81300753 MsG0280011235.01.T01:CDS
TAATAGTTTGATGCAACAGG+TGG 0.645930 2:-81300697 MsG0280011235.01.T01:CDS
GAAGCAGTTTGTGAATATGG+AGG 0.657749 2:-81300787 MsG0280011235.01.T01:CDS
AGTTTCGGTGAATCTTCCGG+TGG 0.669085 2:-81300655 MsG0280011235.01.T01:CDS
ACTCACGGCATCCAATCGGG+AGG 0.700613 2:+81300938 None:intergenic
CCAATCGGGAGGATAATCGG+AGG 0.740590 2:+81300949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCACCTTAAGTTTTT+CGG + Chr2:81300660-81300679 None:intergenic 20.0%
AGAGAAATCCGAAAAACTTA+AGG - Chr2:81300649-81300668 MsG0280011235.01.T01:CDS 30.0%
GTGAAAAAAGCAATCGAAAA+AGG - Chr2:81300671-81300690 MsG0280011235.01.T01:CDS 30.0%
!! TGATTATGGATTGGAAGTTT+CGG - Chr2:81301039-81301058 MsG0280011235.01.T01:CDS 30.0%
AGCAATCGAAAAAGGTAACA+TGG - Chr2:81300679-81300698 MsG0280011235.01.T01:CDS 35.0%
ATCGAAAAAGGTAACATGGA+CGG - Chr2:81300683-81300702 MsG0280011235.01.T01:CDS 35.0%
GGTTAATAGTTTGATGCAAC+AGG - Chr2:81301009-81301028 MsG0280011235.01.T01:CDS 35.0%
TAATAGTTTGATGCAACAGG+TGG - Chr2:81301012-81301031 MsG0280011235.01.T01:CDS 35.0%
TCAACAATCAGCAAATCGAT+GGG - Chr2:81300821-81300840 MsG0280011235.01.T01:CDS 35.0%
! TTGATGAGGATGATTTGTCA+AGG - Chr2:81301122-81301141 MsG0280011235.01.T01:CDS 35.0%
!! CTTTTTCGCATTTTCTTGCT+TGG + Chr2:81300628-81300647 None:intergenic 35.0%
AAACTATTAACCTCCCCTTC+AGG + Chr2:81301001-81301020 None:intergenic 40.0%
CGAGAAGCAGTTTGTGAATA+TGG - Chr2:81300919-81300938 MsG0280011235.01.T01:CDS 40.0%
GAAGCAGTTTGTGAATATGG+AGG - Chr2:81300922-81300941 MsG0280011235.01.T01:CDS 40.0%
GAAGCTTCTGAATCAGATCA+TGG - Chr2:81300571-81300590 MsG0280011235.01.T01:CDS 40.0%
GGGAAACATCGTGAAATCTT+TGG - Chr2:81300841-81300860 MsG0280011235.01.T01:CDS 40.0%
GTCAACAATCAGCAAATCGA+TGG - Chr2:81300820-81300839 MsG0280011235.01.T01:CDS 40.0%
TGTGAATATGGAGGTTCAAG+CGG - Chr2:81300931-81300950 MsG0280011235.01.T01:CDS 40.0%
!! GAAATCTTTGGAATCGTCGT+TGG - Chr2:81300853-81300872 MsG0280011235.01.T01:CDS 40.0%
!! GGAGTTTATGGAAAGTGCTA+TGG - Chr2:81300952-81300971 MsG0280011235.01.T01:CDS 40.0%
!! GTTTATGGAAAGTGCTATGG+CGG - Chr2:81300955-81300974 MsG0280011235.01.T01:CDS 40.0%
!! TTGGCGTTGAAGAGATTTTG+AGG + Chr2:81300609-81300628 None:intergenic 40.0%
!! TTTATGGAAAGTGCTATGGC+GGG - Chr2:81300956-81300975 MsG0280011235.01.T01:CDS 40.0%
CATCCAATCGGGAGGATAAT+CGG + Chr2:81300766-81300785 None:intergenic 45.0%
CTTGCTGAGCTTAAAGCTAG+AGG - Chr2:81301148-81301167 MsG0280011235.01.T01:CDS 45.0%
GGAAGTTTCGGTGAATCTTC+CGG - Chr2:81301051-81301070 MsG0280011235.01.T01:CDS 45.0%
GGGGAATTTGCAGAAGATGT+CGG - Chr2:81300880-81300899 MsG0280011235.01.T01:CDS 45.0%
TAAGGAGAAGGATGCTGAGA+AGG - Chr2:81301099-81301118 MsG0280011235.01.T01:CDS 45.0%
TAGTTTGATGCAACAGGTGG+CGG - Chr2:81301015-81301034 MsG0280011235.01.T01:CDS 45.0%
TCAGCATCCTTCTCCTTAAC+CGG + Chr2:81301097-81301116 None:intergenic 45.0%
! TCATCTGCTCTGTGCGTTTT+CGG + Chr2:81300736-81300755 None:intergenic 45.0%
ACTTCGCTTTCGACTCCTGA+AGG - Chr2:81300983-81301002 MsG0280011235.01.T01:CDS 50.0%
AGTTTCGGTGAATCTTCCGG+TGG - Chr2:81301054-81301073 MsG0280011235.01.T01:CDS 50.0%
CTTCGCTTTCGACTCCTGAA+GGG - Chr2:81300984-81301003 MsG0280011235.01.T01:CDS 50.0%
GGAGGTTCAAGCGGAGTTTA+TGG - Chr2:81300940-81300959 MsG0280011235.01.T01:CDS 50.0%
TGCTCATGCTGTTCCGGTTA+AGG - Chr2:81301081-81301100 MsG0280011235.01.T01:CDS 50.0%
TGCTGTTCCGGTTAAGGAGA+AGG - Chr2:81301087-81301106 MsG0280011235.01.T01:CDS 50.0%
TGTATCGAGACGAGCACTCA+CGG + Chr2:81300789-81300808 None:intergenic 50.0%
TTCGCTTTCGACTCCTGAAG+GGG - Chr2:81300985-81301004 MsG0280011235.01.T01:CDS 50.0%
! TTTGGAATCGTCGTTGGCGA+CGG - Chr2:81300859-81300878 MsG0280011235.01.T01:CDS 50.0%
!! CAACAGGTGGCGGATGATTA+TGG - Chr2:81301025-81301044 MsG0280011235.01.T01:CDS 50.0%
!! GGATGCTGAGAAGGTTGATG+AGG - Chr2:81301108-81301127 MsG0280011235.01.T01:CDS 50.0%
!! GGTGGCGGATGATTATGGAT+TGG - Chr2:81301030-81301049 MsG0280011235.01.T01:CDS 50.0%
AGCACTCACGGCATCCAATC+GGG + Chr2:81300777-81300796 None:intergenic 55.0%
CCAATCGGGAGGATAATCGG+AGG + Chr2:81300763-81300782 None:intergenic 55.0%
CCTCCGATTATCCTCCCGAT+TGG - Chr2:81300760-81300779 MsG0280011235.01.T01:CDS 55.0%
GAGCACTCACGGCATCCAAT+CGG + Chr2:81300778-81300797 None:intergenic 55.0%
GAGTGCTCGTCTCGATACAC+AGG - Chr2:81300790-81300809 MsG0280011235.01.T01:CDS 55.0%
GCAGAAGATGTCGGAGACGA+TGG - Chr2:81300889-81300908 MsG0280011235.01.T01:CDS 55.0%
TTCGGTGAATCTTCCGGTGG+CGG - Chr2:81301057-81301076 MsG0280011235.01.T01:CDS 55.0%
TTGGAATCGTCGTTGGCGAC+GGG - Chr2:81300860-81300879 MsG0280011235.01.T01:CDS 55.0%
ACTCACGGCATCCAATCGGG+AGG + Chr2:81300774-81300793 None:intergenic 60.0%
GCTTTCGACTCCTGAAGGGG+AGG - Chr2:81300988-81301007 MsG0280011235.01.T01:CDS 60.0%
TGGAATCGTCGTTGGCGACG+GGG - Chr2:81300861-81300880 MsG0280011235.01.T01:CDS 60.0%
ACAGCATGAGCAGCCGCCAC+CGG + Chr2:81301073-81301092 None:intergenic 65.0%
GGCGGCTGCTCATGCTGTTC+CGG - Chr2:81301075-81301094 MsG0280011235.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 81300557 81301174 81300557 ID=MsG0280011235.01;Name=MsG0280011235.01
Chr2 mRNA 81300557 81301174 81300557 ID=MsG0280011235.01.T01;Parent=MsG0280011235.01;Name=MsG0280011235.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|205
Chr2 exon 81300557 81301174 81300557 ID=MsG0280011235.01.T01:exon:12057;Parent=MsG0280011235.01.T01
Chr2 CDS 81300557 81301174 81300557 ID=MsG0280011235.01.T01:cds;Parent=MsG0280011235.01.T01
Gene Sequence

>MsG0280011235.01.T01

ATGGGAAACACAGAGAAGCTTCTGAATCAGATCATGGAATTGAAATTCACCTCAAAATCTCTTCAACGCCAAGCAAGAAAATGCGAAAAAGAAGAGAAATCCGAAAAACTTAAGGTGAAAAAAGCAATCGAAAAAGGTAACATGGACGGTGCACGAATCTACGCTGAAAACGCGATCCGAAAACGCACAGAGCAGATGAACTACCTCCGATTATCCTCCCGATTGGATGCCGTGAGTGCTCGTCTCGATACACAGGCGAAGATGTCAACAATCAGCAAATCGATGGGAAACATCGTGAAATCTTTGGAATCGTCGTTGGCGACGGGGAATTTGCAGAAGATGTCGGAGACGATGGATCAATTCGAGAAGCAGTTTGTGAATATGGAGGTTCAAGCGGAGTTTATGGAAAGTGCTATGGCGGGATCGACTTCGCTTTCGACTCCTGAAGGGGAGGTTAATAGTTTGATGCAACAGGTGGCGGATGATTATGGATTGGAAGTTTCGGTGAATCTTCCGGTGGCGGCTGCTCATGCTGTTCCGGTTAAGGAGAAGGATGCTGAGAAGGTTGATGAGGATGATTTGTCAAGGCGTCTTGCTGAGCTTAAAGCTAGAGGTTAA

Protein sequence

>MsG0280011235.01.T01

MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKVKKAIEKGNMDGARIYAENAIRKRTEQMNYLRLSSRLDAVSARLDTQAKMSTISKSMGNIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQAEFMESAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVNLPVAAAHAVPVKEKDAEKVDEDDLSRRLAELKARG*