AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028633.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028633.01.T01 MTR_5g044160 73.797 187 3 1 1 141 26 212 1.04e-91 267
MsG0580028633.01.T01 MTR_4g083060 68.449 187 13 1 1 141 26 212 1.79e-84 249
MsG0580028633.01.T01 MTR_2g091225 60.963 187 27 1 1 141 3 189 4.96e-75 224
MsG0580028633.01.T01 MTR_2g091225 60.963 187 27 1 1 141 26 212 1.03e-74 224
MsG0580028633.01.T01 MTR_8g086270 60.428 187 28 1 1 141 26 212 2.10e-73 220
MsG0580028633.01.T01 MTR_2g015980 59.677 186 30 1 1 141 24 209 1.90e-72 218
MsG0580028633.01.T01 MTR_2g076960 59.358 187 30 2 1 141 27 213 1.19e-71 216
MsG0580028633.01.T01 MTR_3g099380 59.259 189 29 3 1 141 27 215 1.90e-65 200
MsG0580028633.01.T01 MTR_5g064580 57.143 189 33 2 1 141 27 215 3.85e-64 197
MsG0580028633.01.T01 MTR_3g099380 55.367 177 31 2 1 129 27 203 3.95e-57 177
MsG0580028633.01.T01 MTR_3g100620 49.474 190 47 2 1 141 30 219 3.53e-53 169
MsG0580028633.01.T01 MTR_3g014060 41.420 169 55 2 14 138 46 214 5.75e-30 109
MsG0580028633.01.T01 MTR_0137s0020 40.000 185 64 3 1 138 30 214 5.81e-30 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028633.01.T01 AT1G26480 67.380 187 15 1 1 141 29 215 1.64e-82 244
MsG0580028633.01.T01 AT1G34760 62.032 187 25 1 1 141 24 210 9.00e-75 224
MsG0580028633.01.T01 AT1G34760 62.032 187 25 1 1 141 24 210 9.65e-75 223
MsG0580028633.01.T01 AT2G42590 61.497 187 26 1 1 141 26 212 1.57e-73 221
MsG0580028633.01.T01 AT2G42590 61.497 187 26 1 1 141 26 212 1.72e-73 221
MsG0580028633.01.T01 AT2G42590 61.497 187 26 1 1 141 26 212 1.74e-73 221
MsG0580028633.01.T01 AT4G09000 59.259 189 29 3 1 141 29 217 1.29e-66 203
MsG0580028633.01.T01 AT1G35160 59.259 189 29 3 1 141 30 218 4.08e-66 202
MsG0580028633.01.T01 AT1G22300 59.893 187 29 1 1 141 24 210 4.25e-66 202
MsG0580028633.01.T01 AT1G22300 59.893 187 29 1 1 141 24 210 4.30e-66 202
MsG0580028633.01.T01 AT1G22300 59.893 187 29 1 1 141 24 210 6.55e-66 201
MsG0580028633.01.T01 AT1G35160 59.259 189 29 3 1 141 30 218 1.06e-65 202
MsG0580028633.01.T01 AT4G09000 59.259 189 29 3 1 141 29 217 1.62e-65 202
MsG0580028633.01.T01 AT1G78300 58.730 189 30 3 1 141 24 212 2.68e-65 200
MsG0580028633.01.T01 AT5G38480 59.259 189 29 4 1 141 23 211 1.64e-64 197
MsG0580028633.01.T01 AT5G38480 59.259 189 29 4 1 141 23 211 1.64e-64 197
MsG0580028633.01.T01 AT5G38480 59.259 189 29 4 1 141 23 211 1.77e-64 197
MsG0580028633.01.T01 AT3G02520 58.730 189 30 3 1 141 24 212 3.50e-64 197
MsG0580028633.01.T01 AT3G02520 58.730 189 30 3 1 141 24 212 3.50e-64 197
MsG0580028633.01.T01 AT5G16050 53.968 189 39 2 1 141 26 214 5.76e-57 179
MsG0580028633.01.T01 AT5G16050 53.968 189 39 2 1 141 26 214 5.76e-57 179
MsG0580028633.01.T01 AT5G65430 54.023 174 34 1 14 141 42 215 1.26e-54 172
MsG0580028633.01.T01 AT5G65430 54.023 174 34 1 14 141 42 215 1.39e-54 172
MsG0580028633.01.T01 AT5G65430 54.023 174 34 1 14 141 42 215 3.11e-54 172
MsG0580028633.01.T01 AT5G10450 52.299 174 37 1 14 141 42 215 5.59e-54 171
MsG0580028633.01.T01 AT5G10450 52.299 174 37 1 14 141 42 215 5.80e-54 171
MsG0580028633.01.T01 AT5G10450 52.299 174 37 1 14 141 42 215 1.19e-53 170
MsG0580028633.01.T01 AT5G10450 52.299 174 37 1 14 141 42 215 1.50e-53 170
MsG0580028633.01.T01 AT1G78220 42.932 191 62 1 1 144 24 214 2.63e-44 146
MsG0580028633.01.T01 AT1G22290 38.983 177 56 5 4 140 31 195 3.73e-26 98.2
MsG0580028633.01.T01 AT1G22290 39.326 178 56 5 4 141 31 196 5.79e-26 98.2

Find 65 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCGATTGCTTACCTTTC+AGG 0.105745 5:+83847337 None:intergenic
GGACTAGACAAGGTGGAATT+TGG 0.236275 5:-83846233 MsG0580028633.01.T01:three_prime_UTR
TGAGCAGAAGGAGGAGTCTA+AGG 0.249783 5:-83848489 MsG0580028633.01.T01:CDS
GCAGGAAAGGGGATTATTTC+CGG 0.289859 5:-83847631 MsG0580028633.01.T01:intron
ATGCAGAAAATGTGCTTAAA+AGG 0.311470 5:+83846046 None:intergenic
CGCCTCATCAAAAGCTTGTT+TGG 0.319773 5:+83847229 None:intergenic
TAGATACATCACTACCGAAT+TGG 0.327415 5:+83846304 None:intergenic
TACCTCAGGCTCAGCGGGTT+TGG 0.338393 5:+83846579 None:intergenic
CAAAAGCTTGTTTGGCCAAA+TGG 0.349323 5:+83847237 None:intergenic
CAGCATTGATAAGATTTCAT+TGG 0.357814 5:-83846337 MsG0580028633.01.T01:three_prime_UTR
ATAAGATTTCATTGGATCTC+TGG 0.360076 5:-83846329 MsG0580028633.01.T01:three_prime_UTR
AACTTACAGGGCCTGCCATT+TGG 0.370260 5:-83847252 MsG0580028633.01.T01:intron
AGATACATCACTACCGAATT+GGG 0.372560 5:+83846305 None:intergenic
AGGTGCAGATCTTCCGGCTT+CGG 0.381800 5:+83846174 None:intergenic
TGCATTCATCGAGTCTGAAT+TGG 0.390401 5:-83846028 MsG0580028633.01.T01:three_prime_UTR
AATCAGAGGTCCAGAGAGTC+AGG 0.404705 5:+83847134 None:intergenic
TCACTTTACTTAGAAACATA+AGG 0.424485 5:+83846098 None:intergenic
TGAGAGACAACCTGACTCTC+TGG 0.448857 5:-83847144 MsG0580028633.01.T01:three_prime_UTR
AGCTTGTTTGGCCAAATGGC+AGG 0.464126 5:+83847241 None:intergenic
AAAGACAGGTGCAGATCTTC+CGG 0.468481 5:+83846168 None:intergenic
TGATGAGGCGATTGCAGAGT+TGG 0.469932 5:-83847216 MsG0580028633.01.T01:CDS
AATAAATAAATAAGTTTGAA+GGG 0.472994 5:+83846128 None:intergenic
AGACGGATGGGATGTGTTGA+TGG 0.473607 5:+83847409 None:intergenic
AAATAAATAAATAAGTTTGA+AGG 0.475285 5:+83846127 None:intergenic
GCTTCGGTTGGATAACTAAG+AGG 0.493581 5:+83846190 None:intergenic
ATACATACCTCAGGCTCAGC+GGG 0.501637 5:+83846574 None:intergenic
ACCTCTGATTTGCCAGAGGA+TGG 0.501755 5:-83847121 MsG0580028633.01.T01:three_prime_UTR
CTTAGTTATCCAACCGAAGC+CGG 0.505221 5:-83846187 MsG0580028633.01.T01:three_prime_UTR
TTGGATCTCTGGCCCCAATT+CGG 0.506761 5:-83846318 MsG0580028633.01.T01:three_prime_UTR
TCAACACATCCCATCCGTCT+TGG 0.509616 5:-83847406 MsG0580028633.01.T01:CDS
GCAGATCTTCCGGCTTCGGT+TGG 0.512754 5:+83846178 None:intergenic
TCTTGAACTCAGCAAGATAC+CGG 0.525675 5:+83847612 None:intergenic
TTGGTGCACGAAGAGCTTCA+TGG 0.527662 5:-83848529 MsG0580028633.01.T01:CDS
CTCCTCCTTCTGCTCAATTG+AGG 0.527914 5:+83848496 None:intergenic
TATCCCTTACCTCCATCCTC+TGG 0.533069 5:+83847109 None:intergenic
GAGAGGAACCTCCTCTCAGT+TGG 0.543164 5:-83848566 MsG0580028633.01.T01:CDS
TCTAAGATACTCAGTGAAGA+GGG 0.545061 5:-83846285 MsG0580028633.01.T01:three_prime_UTR
GAGTGCAAGTCCAAGACGGA+TGG 0.545908 5:+83847396 None:intergenic
AAGATCAGTGTTTGCAGTGG+CGG 0.557206 5:+83847432 None:intergenic
GACTGACCAAGAGAGAAAAG+AGG 0.559564 5:-83847591 MsG0580028633.01.T01:CDS
TATGTCCTCAATTGAGCAGA+AGG 0.567548 5:-83848501 MsG0580028633.01.T01:CDS
CTATGGATTTGGACTAGACA+AGG 0.572676 5:-83846243 MsG0580028633.01.T01:three_prime_UTR
AGCCAAACCCGCTGAGCCTG+AGG 0.586083 5:-83846581 MsG0580028633.01.T01:intron
CCTTAGTGAAGAGTCGTACA+AGG 0.587245 5:-83847190 MsG0580028633.01.T01:CDS
TTGAACTAACTGTGGAAGAG+AGG 0.589747 5:-83848583 MsG0580028633.01.T01:CDS
GAGCAGAAGGAGGAGTCTAA+GGG 0.590458 5:-83848488 MsG0580028633.01.T01:CDS
ATCTAAGATACTCAGTGAAG+AGG 0.591608 5:-83846286 MsG0580028633.01.T01:three_prime_UTR
CTGGACCTCTGATTTGCCAG+AGG 0.595427 5:-83847125 MsG0580028633.01.T01:three_prime_UTR
GGTTGAATGTATGAAGAAAG+TGG 0.595766 5:-83848618 MsG0580028633.01.T01:CDS
CCTTGTACGACTCTTCACTA+AGG 0.600123 5:+83847190 None:intergenic
GCTGCTGAGCAGTCACTGAA+AGG 0.600437 5:-83847569 MsG0580028633.01.T01:CDS
CTTCGGTTGGATAACTAAGA+GGG 0.603101 5:+83846191 None:intergenic
AGATTATGAACTCTCCTGAA+AGG 0.605075 5:-83847351 MsG0580028633.01.T01:intron
AGTGCAAGTCCAAGACGGAT+GGG 0.618572 5:+83847397 None:intergenic
TCCATCCTCTGGCAAATCAG+AGG 0.621846 5:+83847120 None:intergenic
GATACATCACTACCGAATTG+GGG 0.638690 5:+83846306 None:intergenic
TCTGATTTGCCAGAGGATGG+AGG 0.645454 5:-83847118 MsG0580028633.01.T01:intron
ACTTGATGTTGAACTAACTG+TGG 0.647498 5:-83848591 MsG0580028633.01.T01:CDS
AGGGCACGAGAGCTATGAAG+TGG 0.649523 5:+83846210 None:intergenic
AGTTGAGTGCAAGTCCAAGA+CGG 0.649927 5:+83847392 None:intergenic
GTCCTCAATTGAGCAGAAGG+AGG 0.654997 5:-83848498 MsG0580028633.01.T01:CDS
TATACATACCTCAGGCTCAG+CGG 0.690490 5:+83846573 None:intergenic
TGGATTTGGACTAGACAAGG+TGG 0.693750 5:-83846240 MsG0580028633.01.T01:three_prime_UTR
TGGAAGATCAGTGTTTGCAG+TGG 0.713574 5:+83847429 None:intergenic
GCAGTCACTGAAAGGATACG+AGG 0.737880 5:-83847561 MsG0580028633.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATAAATAAGTTTGA+AGG + Chr5:83848516-83848535 None:intergenic 10.0%
!! AATAAATAAATAAGTTTGAA+GGG + Chr5:83848515-83848534 None:intergenic 10.0%
!!! ATTTTTTGCTTTTAAAAATT+AGG - Chr5:83847608-83847627 MsG0580028633.01.T01:CDS 10.0%
!!! TAATTTTTAAAAGCAAAAAA+TGG + Chr5:83847609-83847628 None:intergenic 10.0%
!!! TTTTTTGCTTTTAAAAATTA+GGG - Chr5:83847609-83847628 MsG0580028633.01.T01:CDS 10.0%
!! AAAAACATAATGTCAAATTT+GGG + Chr5:83848282-83848301 None:intergenic 15.0%
!! AAAATAATATGTCTACTAAA+AGG + Chr5:83846436-83846455 None:intergenic 15.0%
!!! AAATTCTTTTAAGTGAAAAA+AGG - Chr5:83848195-83848214 MsG0580028633.01.T01:intron 15.0%
!!! AATTCTTTTAAGTGAAAAAA+GGG - Chr5:83848196-83848215 MsG0580028633.01.T01:intron 15.0%
!!! TCATTAATAATTATTTCTGA+TGG - Chr5:83847126-83847145 MsG0580028633.01.T01:three_prime_UTR 15.0%
!!! TTAATTAAGCTTTTGTTATT+TGG - Chr5:83847949-83847968 MsG0580028633.01.T01:intron 15.0%
!! AAAAAAAAAAACACAAAGAC+AGG + Chr5:83848489-83848508 None:intergenic 20.0%
!! AAAACATAATGTCAAATTTG+GGG + Chr5:83848281-83848300 None:intergenic 20.0%
!! AAAGTATAGGTATATATTGT+TGG - Chr5:83847686-83847705 MsG0580028633.01.T01:intron 20.0%
!! AAGTATAGGTATATATTGTT+GGG - Chr5:83847687-83847706 MsG0580028633.01.T01:intron 20.0%
!! AGATATAGATACTACATTAT+AGG - Chr5:83846698-83846717 MsG0580028633.01.T01:intron 20.0%
!! CAAAAACATAATGTCAAATT+TGG + Chr5:83848283-83848302 None:intergenic 20.0%
!! TTACTATAGATCTATAACTA+TGG + Chr5:83848151-83848170 None:intergenic 20.0%
!!! CTTTGAATTCTAGTTAATTA+TGG - Chr5:83846670-83846689 MsG0580028633.01.T01:intron 20.0%
! AAAATAAGCATAAGGAAATC+AGG + Chr5:83846986-83847005 None:intergenic 25.0%
! AAACATAATGTCAAATTTGG+GGG + Chr5:83848280-83848299 None:intergenic 25.0%
! AAATAAGCATAAGGAAATCA+GGG + Chr5:83846985-83847004 None:intergenic 25.0%
! AAGAGATTTCATATCACATA+AGG + Chr5:83846933-83846952 None:intergenic 25.0%
! AGTTAGGAGTTTAATAAACA+AGG + Chr5:83846780-83846799 None:intergenic 25.0%
! ATTAAAAATCTTGGATCTCA+GGG + Chr5:83847642-83847661 None:intergenic 25.0%
! CATTATAGGTTATAGATTAC+AGG - Chr5:83846712-83846731 MsG0580028633.01.T01:intron 25.0%
! GTTAGGAGTTTAATAAACAA+GGG + Chr5:83846779-83846798 None:intergenic 25.0%
! GTTTCTATCTATTGTCTAAA+TGG - Chr5:83847356-83847375 MsG0580028633.01.T01:CDS 25.0%
! TCACTTTACTTAGAAACATA+AGG + Chr5:83848545-83848564 None:intergenic 25.0%
!!! TCACAACTGTTTTGATTATT+AGG - Chr5:83847179-83847198 MsG0580028633.01.T01:CDS 25.0%
!!! TTTTTAAGCTTTTCTTGCTA+TGG - Chr5:83848380-83848399 MsG0580028633.01.T01:intron 25.0%
AAACATAGAATCTGTATGAC+TGG - Chr5:83846485-83846504 MsG0580028633.01.T01:intron 30.0%
AAAGGTTAATACTCTCTTAC+AGG - Chr5:83848012-83848031 MsG0580028633.01.T01:intron 30.0%
AAATTTGGGGGTAGAAATTA+TGG + Chr5:83848268-83848287 None:intergenic 30.0%
ACCTATACTTTCAATGTGTT+TGG + Chr5:83847677-83847696 None:intergenic 30.0%
AGTAGGACTTAATCATGAAA+CGG + Chr5:83846572-83846591 None:intergenic 30.0%
ATAAGATTTCATTGGATCTC+TGG - Chr5:83848311-83848330 MsG0580028633.01.T01:intron 30.0%
ATAGGTATATATTGTTGGGT+AGG - Chr5:83847691-83847710 MsG0580028633.01.T01:intron 30.0%
ATGATAAGGACTACGAAATA+TGG - Chr5:83846822-83846841 MsG0580028633.01.T01:intron 30.0%
ATGCAGAAAATGTGCTTAAA+AGG + Chr5:83848597-83848616 None:intergenic 30.0%
ATTTATCGCTATCTTCGTTA+TGG - Chr5:83846399-83846418 MsG0580028633.01.T01:intron 30.0%
GATTAAAAATCTTGGATCTC+AGG + Chr5:83847643-83847662 None:intergenic 30.0%
GTTGGATATAAAAACGTCAT+TGG - Chr5:83846092-83846111 MsG0580028633.01.T01:three_prime_UTR 30.0%
TACTTTCAATGTGTTTGGTT+TGG + Chr5:83847672-83847691 None:intergenic 30.0%
TATAGGGTTTCAGATAAAGA+TGG - Chr5:83846635-83846654 MsG0580028633.01.T01:intron 30.0%
TATATTCCGCTCAAGTTATA+GGG - Chr5:83846619-83846638 MsG0580028633.01.T01:intron 30.0%
TCTATCTATTGTCTAAATGG+TGG - Chr5:83847359-83847378 MsG0580028633.01.T01:CDS 30.0%
TGGATAATTTATCCAATAGC+AGG + Chr5:83847867-83847886 None:intergenic 30.0%
TGTGATATGAAATCTCTTTC+TGG - Chr5:83846935-83846954 MsG0580028633.01.T01:intron 30.0%
TTATATTCCGCTCAAGTTAT+AGG - Chr5:83846618-83846637 MsG0580028633.01.T01:intron 30.0%
! ACAATATGACTATGGTTTTC+TGG + Chr5:83847887-83847906 None:intergenic 30.0%
! ACCAAACACATTGAAAGTAT+AGG - Chr5:83847673-83847692 MsG0580028633.01.T01:intron 30.0%
! GAGATCCAAGATTTTTAATC+TGG - Chr5:83847643-83847662 MsG0580028633.01.T01:intron 30.0%
! TCAGAATATCACACAGATTT+TGG + Chr5:83846235-83846254 None:intergenic 30.0%
! TTCACTGTTAGATTCTAGTT+AGG + Chr5:83846796-83846815 None:intergenic 30.0%
!! CAGCATTGATAAGATTTCAT+TGG - Chr5:83848303-83848322 MsG0580028633.01.T01:intron 30.0%
!! GGTATGTATAAGATTGGTTT+CGG - Chr5:83848080-83848099 MsG0580028633.01.T01:intron 30.0%
!!! CCTAATTTTTTGACAAGGTT+GGG + Chr5:83847821-83847840 None:intergenic 30.0%
!!! CTAATTTTTTGACAAGGTTG+GGG + Chr5:83847820-83847839 None:intergenic 30.0%
!!! CTCATCCTAATTTTTTGACA+AGG + Chr5:83847826-83847845 None:intergenic 30.0%
!!! TCCTAATTTTTTGACAAGGT+TGG + Chr5:83847822-83847841 None:intergenic 30.0%
ACAGTGAAGAACTCATGATA+AGG - Chr5:83846808-83846827 MsG0580028633.01.T01:intron 35.0%
ACCAATCTTATACATACCTC+AGG + Chr5:83848078-83848097 None:intergenic 35.0%
ACTTGATGTTGAACTAACTG+TGG - Chr5:83846049-83846068 MsG0580028633.01.T01:three_prime_UTR 35.0%
AGATACATCACTACCGAATT+GGG + Chr5:83848338-83848357 None:intergenic 35.0%
AGATTATGAACTCTCCTGAA+AGG - Chr5:83847289-83847308 MsG0580028633.01.T01:intron 35.0%
AGGTGGATAACAAATATGCA+AGG + Chr5:83846536-83846555 None:intergenic 35.0%
CCCAACCTTGTCAAAAAATT+AGG - Chr5:83847818-83847837 MsG0580028633.01.T01:intron 35.0%
CCTGCATCAAAATAAGCATA+AGG + Chr5:83846994-83847013 None:intergenic 35.0%
GAAGATAGCGATAAATCTGA+TGG + Chr5:83846395-83846414 None:intergenic 35.0%
GCAAAAAATGGAGATCTTGA+AGG + Chr5:83847597-83847616 None:intergenic 35.0%
GGCATCCAGATTAAAAATCT+TGG + Chr5:83847651-83847670 None:intergenic 35.0%
GGTTGAATGTATGAAGAAAG+TGG - Chr5:83846022-83846041 MsG0580028633.01.T01:three_prime_UTR 35.0%
GTAGGACTTAATCATGAAAC+GGG + Chr5:83846571-83846590 None:intergenic 35.0%
GTATATATTGTTGGGTAGGT+TGG - Chr5:83847695-83847714 MsG0580028633.01.T01:intron 35.0%
TAGATACATCACTACCGAAT+TGG + Chr5:83848339-83848358 None:intergenic 35.0%
TCCAATGACTCATTCATAGT+AGG + Chr5:83846589-83846608 None:intergenic 35.0%
TCCTACTATGAATGAGTCAT+TGG - Chr5:83846585-83846604 MsG0580028633.01.T01:three_prime_UTR 35.0%
TTAATACTCTCTTACAGGTG+AGG - Chr5:83848017-83848036 MsG0580028633.01.T01:intron 35.0%
! ATCTAAGATACTCAGTGAAG+AGG - Chr5:83848354-83848373 MsG0580028633.01.T01:intron 35.0%
! ATGGTGGTGATAAAACTTAC+AGG - Chr5:83847375-83847394 MsG0580028633.01.T01:CDS 35.0%
! CGTTTTTATATCCAACTGAG+AGG + Chr5:83846088-83846107 None:intergenic 35.0%
! CTCTGGAAACAATATGACTA+TGG + Chr5:83847895-83847914 None:intergenic 35.0%
! TCCTACTAAAGAGGTTCTAA+AGG - Chr5:83847994-83848013 MsG0580028633.01.T01:intron 35.0%
! TCTAAGATACTCAGTGAAGA+GGG - Chr5:83848355-83848374 MsG0580028633.01.T01:intron 35.0%
! TGGTGGTGATAAAACTTACA+GGG - Chr5:83847376-83847395 MsG0580028633.01.T01:CDS 35.0%
! TTTTATATCCAACTGAGAGG+AGG + Chr5:83846085-83846104 None:intergenic 35.0%
!! ACCTTTAGAACCTCTTTAGT+AGG + Chr5:83847998-83848017 None:intergenic 35.0%
!! AGCTTTTCTTGCTATGGATT+TGG - Chr5:83848386-83848405 MsG0580028633.01.T01:intron 35.0%
!! TTGCATATTTGTTATCCACC+TGG - Chr5:83846535-83846554 MsG0580028633.01.T01:intron 35.0%
!! TTTGGCTTCTTCAGCTTTTA+TGG + Chr5:83848046-83848065 None:intergenic 35.0%
!!! CCTTATGCTTATTTTGATGC+AGG - Chr5:83846991-83847010 MsG0580028633.01.T01:intron 35.0%
!!! CTTATTTTGATGCAGGAAAG+GGG - Chr5:83846998-83847017 MsG0580028633.01.T01:intron 35.0%
!!! GCTTATTTTGATGCAGGAAA+GGG - Chr5:83846997-83847016 MsG0580028633.01.T01:intron 35.0%
!!! TGCTTATTTTGATGCAGGAA+AGG - Chr5:83846996-83847015 MsG0580028633.01.T01:intron 35.0%
AAATCTGATGGCCAACAAGT+CGG + Chr5:83846383-83846402 None:intergenic 40.0%
CATAGAATCTGTATGACTGG+TGG - Chr5:83846488-83846507 MsG0580028633.01.T01:intron 40.0%
CGATTCTCTCATACTTTGCA+TGG - Chr5:83847320-83847339 MsG0580028633.01.T01:intron 40.0%
CTGAAACCCTATAACTTGAG+CGG + Chr5:83846628-83846647 None:intergenic 40.0%
CTTCGGTTGGATAACTAAGA+GGG + Chr5:83848452-83848471 None:intergenic 40.0%
GAATCGATTGCTTACCTTTC+AGG + Chr5:83847306-83847325 None:intergenic 40.0%
GACAATTATTGACCAGCATC+TGG - Chr5:83846253-83846272 MsG0580028633.01.T01:three_prime_UTR 40.0%
GATACATCACTACCGAATTG+GGG + Chr5:83848337-83848356 None:intergenic 40.0%
GATCAAGAGTTACTGCCAAA+AGG - Chr5:83846194-83846213 MsG0580028633.01.T01:three_prime_UTR 40.0%
GATTACTCTTGCGGTGATTA+GGG + Chr5:83847775-83847794 None:intergenic 40.0%
GCCTGAGGTATGTATAAGAT+TGG - Chr5:83848074-83848093 MsG0580028633.01.T01:intron 40.0%
GGACTACGAAATATGGAAAC+TGG - Chr5:83846829-83846848 MsG0580028633.01.T01:intron 40.0%
TAGAACTGCTCCTACTAAAG+AGG - Chr5:83847985-83848004 MsG0580028633.01.T01:intron 40.0%
TATGTCCTCAATTGAGCAGA+AGG - Chr5:83846139-83846158 MsG0580028633.01.T01:three_prime_UTR 40.0%
TCACTAGAAGAAACTCACTC+TGG + Chr5:83847912-83847931 None:intergenic 40.0%
TCTTGAACTCAGCAAGATAC+CGG + Chr5:83847031-83847050 None:intergenic 40.0%
TGATTACTCTTGCGGTGATT+AGG + Chr5:83847776-83847795 None:intergenic 40.0%
TGATTAGGGACAAGTCAACT+TGG + Chr5:83847761-83847780 None:intergenic 40.0%
TGCATTCATCGAGTCTGAAT+TGG - Chr5:83848612-83848631 MsG0580028633.01.T01:CDS 40.0%
TTGAACTAACTGTGGAAGAG+AGG - Chr5:83846057-83846076 MsG0580028633.01.T01:three_prime_UTR 40.0%
TTGGCATTCCATATCCTAGT+GGG - Chr5:83847714-83847733 MsG0580028633.01.T01:intron 40.0%
! AAGTCAACTTGGTGGACAAT+GGG + Chr5:83847750-83847769 None:intergenic 40.0%
! CAAAAGCTTGTTTGGCCAAA+TGG + Chr5:83847406-83847425 None:intergenic 40.0%
! CTATGGATTTGGACTAGACA+AGG - Chr5:83848397-83848416 MsG0580028633.01.T01:intron 40.0%
! TCAAATCCTTTCCGACTTGT+TGG - Chr5:83846369-83846388 MsG0580028633.01.T01:intron 40.0%
! TTTTTGACAAGGTTGGGGTA+AGG + Chr5:83847815-83847834 None:intergenic 40.0%
AAAGACAGGTGCAGATCTTC+CGG + Chr5:83848475-83848494 None:intergenic 45.0%
AAGATCAGTGTTTGCAGTGG+CGG + Chr5:83847211-83847230 None:intergenic 45.0%
AGTTGAGTGCAAGTCCAAGA+CGG + Chr5:83847251-83847270 None:intergenic 45.0%
CATTTGTCCCCACTAGGATA+TGG + Chr5:83847725-83847744 None:intergenic 45.0%
CCTTAGTGAAGAGTCGTACA+AGG - Chr5:83847450-83847469 MsG0580028633.01.T01:intron 45.0%
CCTTGTACGACTCTTCACTA+AGG + Chr5:83847453-83847472 None:intergenic 45.0%
CGAGTGGATGATTACTCTTG+CGG + Chr5:83847784-83847803 None:intergenic 45.0%
CTAAGAACTGTGCCTGCTAT+TGG - Chr5:83847852-83847871 MsG0580028633.01.T01:intron 45.0%
CTTAGTTATCCAACCGAAGC+CGG - Chr5:83848453-83848472 MsG0580028633.01.T01:intron 45.0%
GACTGACCAAGAGAGAAAAG+AGG - Chr5:83847049-83847068 MsG0580028633.01.T01:intron 45.0%
GAGTTACTGCCAAAAGGTTG+AGG - Chr5:83846200-83846219 MsG0580028633.01.T01:three_prime_UTR 45.0%
GCAGGAAAGGGGATTATTTC+CGG - Chr5:83847009-83847028 MsG0580028633.01.T01:intron 45.0%
GCTTCGGTTGGATAACTAAG+AGG + Chr5:83848453-83848472 None:intergenic 45.0%
GGACTAGACAAGGTGGAATT+TGG - Chr5:83848407-83848426 MsG0580028633.01.T01:intron 45.0%
GGGTAAGGATACAAATCGAG+TGG + Chr5:83847800-83847819 None:intergenic 45.0%
GTTGGCATTCCATATCCTAG+TGG - Chr5:83847713-83847732 MsG0580028633.01.T01:intron 45.0%
TATACATACCTCAGGCTCAG+CGG + Chr5:83848070-83848089 None:intergenic 45.0%
TGGAAGATCAGTGTTTGCAG+TGG + Chr5:83847214-83847233 None:intergenic 45.0%
TGGCATTCCATATCCTAGTG+GGG - Chr5:83847715-83847734 MsG0580028633.01.T01:intron 45.0%
TTACTGCCAAAAGGTTGAGG+AGG - Chr5:83846203-83846222 MsG0580028633.01.T01:three_prime_UTR 45.0%
TTAGGGACAAGTCAACTTGG+TGG + Chr5:83847758-83847777 None:intergenic 45.0%
! AGAGTTCCTCCTCAACCTTT+TGG + Chr5:83846212-83846231 None:intergenic 45.0%
! CAAGTCAACTTGGTGGACAA+TGG + Chr5:83847751-83847770 None:intergenic 45.0%
! CGCCTCATCAAAAGCTTGTT+TGG + Chr5:83847414-83847433 None:intergenic 45.0%
! GAAAACATTAGCTTCTGCCG+AGG + Chr5:83846291-83846310 None:intergenic 45.0%
! GGCCAAACAAGCTTTTGATG+AGG - Chr5:83847409-83847428 MsG0580028633.01.T01:CDS 45.0%
! TGGATTTGGACTAGACAAGG+TGG - Chr5:83848400-83848419 MsG0580028633.01.T01:intron 45.0%
!! TTTCTGGCTCACACGTACTT+TGG - Chr5:83846951-83846970 MsG0580028633.01.T01:intron 45.0%
AACTTACAGGGCCTGCCATT+TGG - Chr5:83847388-83847407 MsG0580028633.01.T01:CDS 50.0%
AATCAGAGGTCCAGAGAGTC+AGG + Chr5:83847509-83847528 None:intergenic 50.0%
AGACGGATGGGATGTGTTGA+TGG + Chr5:83847234-83847253 None:intergenic 50.0%
AGTGCAAGTCCAAGACGGAT+GGG + Chr5:83847246-83847265 None:intergenic 50.0%
ATACATACCTCAGGCTCAGC+GGG + Chr5:83848069-83848088 None:intergenic 50.0%
CTCCTCCTTCTGCTCAATTG+AGG + Chr5:83846147-83846166 None:intergenic 50.0%
GAGCAGAAGGAGGAGTCTAA+GGG - Chr5:83846152-83846171 MsG0580028633.01.T01:three_prime_UTR 50.0%
GCAGTCACTGAAAGGATACG+AGG - Chr5:83847079-83847098 MsG0580028633.01.T01:intron 50.0%
GTCCTCAATTGAGCAGAAGG+AGG - Chr5:83846142-83846161 MsG0580028633.01.T01:three_prime_UTR 50.0%
TATCCCTTACCTCCATCCTC+TGG + Chr5:83847534-83847553 None:intergenic 50.0%
TCAACACATCCCATCCGTCT+TGG - Chr5:83847234-83847253 MsG0580028633.01.T01:CDS 50.0%
TCCATCCTCTGGCAAATCAG+AGG + Chr5:83847523-83847542 None:intergenic 50.0%
TGAGAGACAACCTGACTCTC+TGG - Chr5:83847496-83847515 MsG0580028633.01.T01:intron 50.0%
TGAGCAGAAGGAGGAGTCTA+AGG - Chr5:83846151-83846170 MsG0580028633.01.T01:three_prime_UTR 50.0%
TGATGAGGCGATTGCAGAGT+TGG - Chr5:83847424-83847443 MsG0580028633.01.T01:CDS 50.0%
TGATGGCCAACAAGTCGGAA+AGG + Chr5:83846378-83846397 None:intergenic 50.0%
TTGCCAGAGGATGGAGGTAA+GGG - Chr5:83847528-83847547 MsG0580028633.01.T01:intron 50.0%
TTGGATCTCTGGCCCCAATT+CGG - Chr5:83848322-83848341 MsG0580028633.01.T01:intron 50.0%
TTGGTGCACGAAGAGCTTCA+TGG - Chr5:83846111-83846130 MsG0580028633.01.T01:three_prime_UTR 50.0%
TTTGCCAGAGGATGGAGGTA+AGG - Chr5:83847527-83847546 MsG0580028633.01.T01:intron 50.0%
! AACATTAGCTTCTGCCGAGG+TGG + Chr5:83846288-83846307 None:intergenic 50.0%
! ACCTCTGATTTGCCAGAGGA+TGG - Chr5:83847519-83847538 MsG0580028633.01.T01:intron 50.0%
! AGCTTGTTTGGCCAAATGGC+AGG + Chr5:83847402-83847421 None:intergenic 50.0%
! CAGCAGCCTCTTTTCTCTCT+TGG + Chr5:83847058-83847077 None:intergenic 50.0%
! TCTGATTTGCCAGAGGATGG+AGG - Chr5:83847522-83847541 MsG0580028633.01.T01:intron 50.0%
AGGGCACGAGAGCTATGAAG+TGG + Chr5:83848433-83848452 None:intergenic 55.0%
AGGTGCAGATCTTCCGGCTT+CGG + Chr5:83848469-83848488 None:intergenic 55.0%
GAAACGGGTGCTCATCTACC+AGG + Chr5:83846556-83846575 None:intergenic 55.0%
GAGAGGAACCTCCTCTCAGT+TGG - Chr5:83846074-83846093 MsG0580028633.01.T01:three_prime_UTR 55.0%
GAGTGCAAGTCCAAGACGGA+TGG + Chr5:83847247-83847266 None:intergenic 55.0%
GCTGCTGAGCAGTCACTGAA+AGG - Chr5:83847071-83847090 MsG0580028633.01.T01:intron 55.0%
TGGAAGCAGGAACCAGATGC+TGG + Chr5:83846268-83846287 None:intergenic 55.0%
! CTGGACCTCTGATTTGCCAG+AGG - Chr5:83847515-83847534 MsG0580028633.01.T01:intron 55.0%
! TCTGGTTCCTGCTTCCACCT+CGG - Chr5:83846271-83846290 MsG0580028633.01.T01:three_prime_UTR 55.0%
!! TGGGAGCATTTGTCCCCACT+AGG + Chr5:83847731-83847750 None:intergenic 55.0%
ACGGGTGCTCATCTACCAGG+TGG + Chr5:83846553-83846572 None:intergenic 60.0%
GCAGATCTTCCGGCTTCGGT+TGG + Chr5:83848465-83848484 None:intergenic 60.0%
! TACCTCAGGCTCAGCGGGTT+TGG + Chr5:83848064-83848083 None:intergenic 60.0%
AGCCAAACCCGCTGAGCCTG+AGG - Chr5:83848059-83848078 MsG0580028633.01.T01:intron 65.0%
GCTTCTGCCGAGGTGGAAGC+AGG + Chr5:83846281-83846300 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 83846020 83848642 83846020 ID=MsG0580028633.01;Name=MsG0580028633.01
Chr5 mRNA 83846020 83848642 83846020 ID=MsG0580028633.01.T01;Parent=MsG0580028633.01;Name=MsG0580028633.01.T01;_AED=0.38;_eAED=0.38;_QI=0|0.8|0.83|1|0.6|0.5|6|443|150
Chr5 exon 83846020 83846356 83846020 ID=MsG0580028633.01.T01:exon:17109;Parent=MsG0580028633.01.T01
Chr5 exon 83846582 83846628 83846582 ID=MsG0580028633.01.T01:exon:17108;Parent=MsG0580028633.01.T01
Chr5 exon 83847119 83847265 83847119 ID=MsG0580028633.01.T01:exon:17107;Parent=MsG0580028633.01.T01
Chr5 exon 83847352 83847461 83847352 ID=MsG0580028633.01.T01:exon:17106;Parent=MsG0580028633.01.T01
Chr5 exon 83847562 83847649 83847562 ID=MsG0580028633.01.T01:exon:17105;Parent=MsG0580028633.01.T01
Chr5 exon 83848476 83848642 83848476 ID=MsG0580028633.01.T01:exon:17104;Parent=MsG0580028633.01.T01
Chr5 CDS 83848476 83848642 83848476 ID=MsG0580028633.01.T01:cds;Parent=MsG0580028633.01.T01
Chr5 CDS 83847562 83847649 83847562 ID=MsG0580028633.01.T01:cds;Parent=MsG0580028633.01.T01
Chr5 CDS 83847352 83847461 83847352 ID=MsG0580028633.01.T01:cds;Parent=MsG0580028633.01.T01
Chr5 CDS 83847178 83847265 83847178 ID=MsG0580028633.01.T01:cds;Parent=MsG0580028633.01.T01
Chr5 three_prime_UTR 83847119 83847177 83847119 ID=MsG0580028633.01.T01:three_prime_utr;Parent=MsG0580028633.01.T01
Chr5 three_prime_UTR 83846582 83846628 83846582 ID=MsG0580028633.01.T01:three_prime_utr;Parent=MsG0580028633.01.T01
Chr5 three_prime_UTR 83846020 83846356 83846020 ID=MsG0580028633.01.T01:three_prime_utr;Parent=MsG0580028633.01.T01
Gene Sequence

>MsG0580028633.01.T01

ATGGTTGAATGTATGAAGAAAGTGGCAAAACTTGATGTTGAACTAACTGTGGAAGAGAGGAACCTCCTCTCAGTTGGATATAAAAACGTCATTGGTGCACGAAGAGCTTCATGGCGCATTATGTCCTCAATTGAGCAGAAGGAGGAGTCTAAGGGTATGAACACAATGAAAGGGGATTATTTCCGGTATCTTGCTGAGTTCAAGACTGACCAAGAGAGAAAAGAGGCTGCTGAGCAGTCACTGAAAGGATACGAGGCTGCTTCCGCCACTGCAAACACTGATCTTCCATCAACACATCCCATCCGTCTTGGACTTGCACTCAACTTCTCTGTCTTTTATTATGAGATTATGAACTCTCCTGAAAGGGCCTGCCATTTGGCCAAACAAGCTTTTGATGAGGCGATTGCAGAGTTGGATACCTTAGTGAAGAGTCGTACAAGGACAGCACTTTGA
Protein sequence

>MsG0580028633.01.T01

MVECMKKVAKLDVELTVEERNLLSVGYKNVIGARRASWRIMSSIEQKEESKGMNTMKGDYFRYLAEFKTDQERKEAAEQSLKGYEAASATANTDLPSTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAIAELDTLVKSRTRTAL*