AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021301.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021301.01.T01 MTR_4g071150 98.026 152 1 1 1 150 1 152 1.02e-100 285
MsG0480021301.01.T01 MTR_2g096610 87.333 150 17 1 1 150 1 148 4.59e-83 241
MsG0480021301.01.T01 MTR_4g064005 94.000 150 7 1 1 150 1 148 1.38e-82 239
MsG0480021301.01.T01 MTR_4g063410 94.000 150 7 1 1 150 1 148 2.81e-82 239
MsG0480021301.01.T01 MTR_2g096570 91.558 154 8 2 1 150 1 153 5.87e-82 238
MsG0480021301.01.T01 MTR_4g063280 92.424 132 9 1 20 150 14 145 2.96e-81 236
MsG0480021301.01.T01 MTR_4g064967 93.333 150 8 1 1 150 1 148 3.10e-80 234
MsG0480021301.01.T01 MTR_8g086640 96.212 132 5 0 1 132 1 132 4.44e-75 221
MsG0480021301.01.T01 MTR_7g113160 79.699 133 26 1 10 142 13 144 5.21e-71 210
MsG0480021301.01.T01 MTR_7g108320 75.000 128 26 3 4 130 6 128 7.88e-60 182
MsG0480021301.01.T01 MTR_7g114060 74.219 128 29 1 3 130 2 125 8.16e-60 182
MsG0480021301.01.T01 MTR_7g114040 74.219 128 29 1 3 130 2 125 8.16e-60 182
MsG0480021301.01.T01 MTR_7g093910 73.228 127 31 1 4 130 5 128 9.70e-60 181
MsG0480021301.01.T01 MTR_2g082510 70.543 129 38 0 11 139 3 131 3.33e-59 180
MsG0480021301.01.T01 MTR_2g082220 73.950 119 31 0 11 129 3 121 1.72e-57 176
MsG0480021301.01.T01 MTR_6g005150 62.992 127 33 3 24 149 26 139 3.28e-47 150
MsG0480021301.01.T01 MTR_4g063300 75.238 105 21 1 45 149 10 109 3.46e-46 146
MsG0480021301.01.T01 MTR_7g114050 68.644 118 22 3 17 130 10 116 2.27e-45 145
MsG0480021301.01.T01 MTR_1g011750 60.714 112 42 2 21 131 23 133 3.72e-36 121
MsG0480021301.01.T01 MTR_1g011850 60.714 112 42 2 21 131 23 133 3.72e-36 121
MsG0480021301.01.T01 MTR_1g114050 60.550 109 41 2 21 128 20 127 3.01e-35 119
MsG0480021301.01.T01 MTR_1g071040 62.500 104 37 2 26 128 28 130 1.59e-34 117
MsG0480021301.01.T01 MTR_1g069770 48.958 96 48 1 45 139 9 104 5.82e-25 92.4
MsG0480021301.01.T01 MTR_4g048490 44.762 105 29 2 24 128 26 101 1.75e-16 70.9
MsG0480021301.01.T01 MTR_7g010440 70.588 51 15 0 78 128 122 172 2.30e-16 74.3
MsG0480021301.01.T01 MTR_4g005910 74.419 43 11 0 87 129 4 46 3.30e-16 68.6
MsG0480021301.01.T01 MTR_7g027610 41.525 118 42 4 20 135 10 102 5.78e-15 67.0
MsG0480021301.01.T01 MTR_0653s0030 54.545 55 25 0 55 109 43 97 4.70e-12 60.5
MsG0480021301.01.T01 MTR_2g096590 70.000 40 12 0 110 149 12 51 5.47e-12 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021301.01.T01 AT5G02560 79.739 153 28 1 1 150 1 153 1.54e-77 227
MsG0480021301.01.T01 AT5G27670 76.316 152 32 3 1 150 1 150 3.46e-74 218
MsG0480021301.01.T01 AT5G02560 68.927 177 28 2 1 150 1 177 1.10e-72 216
MsG0480021301.01.T01 AT5G59870 75.000 148 36 1 4 150 3 150 9.34e-72 212
MsG0480021301.01.T01 AT1G51060 76.667 120 28 0 11 130 3 122 9.51e-61 184
MsG0480021301.01.T01 AT4G27230 71.538 130 32 1 11 140 3 127 1.49e-59 181
MsG0480021301.01.T01 AT4G27230 71.538 130 32 1 11 140 3 127 1.49e-59 181
MsG0480021301.01.T01 AT3G20670 75.630 119 29 0 11 129 3 121 4.96e-59 179
MsG0480021301.01.T01 AT1G08880 71.654 127 33 1 7 130 2 128 3.60e-58 177
MsG0480021301.01.T01 AT5G54640 74.790 119 30 0 11 129 3 121 4.69e-58 177
MsG0480021301.01.T01 AT1G54690 72.868 129 29 3 3 130 5 128 1.24e-57 176
MsG0480021301.01.T01 AT2G38810 60.714 112 42 2 21 131 25 135 7.22e-37 123
MsG0480021301.01.T01 AT2G38810 60.714 112 42 2 21 131 25 135 7.22e-37 123
MsG0480021301.01.T01 AT2G38810 60.714 112 42 2 21 131 25 135 7.22e-37 123
MsG0480021301.01.T01 AT2G38810 60.714 112 42 2 21 131 25 135 7.22e-37 123
MsG0480021301.01.T01 AT1G52740 62.857 105 37 2 25 128 27 130 6.40e-35 118
MsG0480021301.01.T01 AT3G54560 60.185 108 41 2 25 131 29 135 1.29e-34 118
MsG0480021301.01.T01 AT3G54560 60.185 108 41 2 25 131 29 135 1.29e-34 118
MsG0480021301.01.T01 AT4G13570 48.913 92 45 2 32 122 28 118 2.04e-20 81.3
MsG0480021301.01.T01 AT4G13570 48.352 91 45 2 33 122 35 124 5.83e-20 80.1

Find 56 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.241789 4:-56478996 None:intergenic
AATCAGATCTAAGCCTTCTT+TGG 0.242120 4:-56479017 None:intergenic
GTCTTCTTAGGCAACAGAAT+TGG 0.279189 4:-56478945 None:intergenic
TTAGCCCTTTCAGTCTTCTT+AGG 0.279305 4:-56478957 None:intergenic
CGATTCTTCCGACTGGGAAT+TGG 0.285182 4:-56478677 None:intergenic
CATTCCAAGGCATTTGTTGC+TGG 0.294047 4:+56478842 MsG0480021301.01.T01:CDS
CTTGTCACCGACTTCTTCCT+TGG 0.303917 4:-56478636 None:intergenic
GGCTCCCTTCTTTGTTGTCT+TGG 0.304409 4:-56478592 None:intergenic
GAGCATATCTACCTTTCTTC+AGG 0.354180 4:-56478707 None:intergenic
TGCTGCTGAGGTTCTTGAAT+TGG 0.361111 4:+56478782 MsG0480021301.01.T01:CDS
GAGCTTGGGAAGTTGCTTGC+TGG 0.378028 4:+56478882 MsG0480021301.01.T01:CDS
GGCATTTGTTGCTGGCTGTT+AGG 0.378218 4:+56478850 MsG0480021301.01.T01:CDS
TCTTCTTAGGCAACAGAATT+GGG 0.398360 4:-56478944 None:intergenic
GAAGGGAGCCGGTGGAAGAA+AGG 0.417242 4:+56478605 MsG0480021301.01.T01:CDS
AACAGAATTGGGTTGATGTT+CGG 0.418504 4:-56478933 None:intergenic
AAGGGAGCCGGTGGAAGAAA+GGG 0.429527 4:+56478606 MsG0480021301.01.T01:CDS
CAATTCCCAGTCGGAAGAAT+CGG 0.431861 4:+56478678 MsG0480021301.01.T01:CDS
AGAACTGCTGCTAGGTAAAC+CGG 0.439504 4:-56478750 None:intergenic
ACCTCCTCCCTTTCTTCCAC+CGG 0.471747 4:-56478613 None:intergenic
GCGTGTCGGCACCGGTGCTC+CGG 0.486643 4:+56478731 MsG0480021301.01.T01:CDS
GAATTGGAGACCGGCTCTGA+TGG 0.486869 4:-56478661 None:intergenic
TCTTGAATATCTTGCTGCTG+AGG 0.496391 4:+56478770 MsG0480021301.01.T01:CDS
AAGCACCAAGACAACAAAGA+AGG 0.501816 4:+56478587 MsG0480021301.01.T01:CDS
TATGCTCAGCGTGTCGGCAC+CGG 0.502840 4:+56478723 MsG0480021301.01.T01:CDS
GTTAGGAATGATGAAGAGCT+TGG 0.506747 4:+56478867 MsG0480021301.01.T01:CDS
AGCAAGGAACCAAAGAAGGC+TGG 0.510603 4:+56478987 MsG0480021301.01.T01:CDS
GCTGAGGTTCTTGAATTGGC+AGG 0.519701 4:+56478786 MsG0480021301.01.T01:CDS
CTCGTGATAACAAGAAGAAT+AGG 0.522113 4:+56478817 MsG0480021301.01.T01:CDS
TTAGGAATGATGAAGAGCTT+GGG 0.524474 4:+56478868 MsG0480021301.01.T01:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 4:+56479004 MsG0480021301.01.T01:CDS
ACAGCCAGCAACAAATGCCT+TGG 0.526781 4:-56478846 None:intergenic
ATCGGTCGATACCTGAAGAA+AGG 0.532583 4:+56478696 MsG0480021301.01.T01:CDS
TGCTAGGTAAACCGGAGCAC+CGG 0.537567 4:-56478742 None:intergenic
TATCGACCGATTCTTCCGAC+TGG 0.549955 4:-56478684 None:intergenic
TGGTAGCAAGGAACCAAAGA+AGG 0.550474 4:+56478983 MsG0480021301.01.T01:CDS
TGTTGCCTAAGAAGACTGAA+AGG 0.556495 4:+56478952 MsG0480021301.01.T01:CDS
AGCACCAAGACAACAAAGAA+GGG 0.558080 4:+56478588 MsG0480021301.01.T01:CDS
GAAGAAAGGGAGGAGGTCCA+AGG 0.558674 4:+56478619 MsG0480021301.01.T01:CDS
TCCGACTGGGAATTGGAGAC+CGG 0.563841 4:-56478670 None:intergenic
AAGAACACCACCATGAGCTA+TGG 0.573393 4:-56478910 None:intergenic
AAGACAACAAAGAAGGGAGC+CGG 0.578427 4:+56478594 MsG0480021301.01.T01:CDS
GATATTCAAGAACTGCTGCT+AGG 0.583613 4:-56478758 None:intergenic
GCTGGTGTTACCATAGCTCA+TGG 0.586669 4:+56478900 MsG0480021301.01.T01:CDS
GTGACAAGATCCATCAGAGC+CGG 0.589197 4:+56478651 MsG0480021301.01.T01:CDS
GTTGCCTAAGAAGACTGAAA+GGG 0.589268 4:+56478953 MsG0480021301.01.T01:CDS
GCCGGTGGAAGAAAGGGAGG+AGG 0.593863 4:+56478612 MsG0480021301.01.T01:CDS
GGTAGATATGCTCAGCGTGT+CGG 0.594469 4:+56478717 MsG0480021301.01.T01:CDS
AAGGGCTAACACTGGTAGCA+AGG 0.594830 4:+56478971 MsG0480021301.01.T01:CDS
ACAACAAAGAAGGGAGCCGG+TGG 0.595449 4:+56478597 MsG0480021301.01.T01:CDS
GGAGCCGGTGGAAGAAAGGG+AGG 0.604037 4:+56478609 MsG0480021301.01.T01:CDS
ATCGACCGATTCTTCCGACT+GGG 0.613102 4:-56478683 None:intergenic
AGGAGGTCCAAGGAAGAAGT+CGG 0.620460 4:+56478629 MsG0480021301.01.T01:CDS
GCCGGTCTCCAATTCCCAGT+CGG 0.630576 4:+56478669 MsG0480021301.01.T01:CDS
AGAAGAATAGGATCATTCCA+AGG 0.652341 4:+56478829 MsG0480021301.01.T01:CDS
AAGACTGAAAGGGCTAACAC+TGG 0.665786 4:+56478963 MsG0480021301.01.T01:CDS
GGTGTTACCATAGCTCATGG+TGG 0.742529 4:+56478903 MsG0480021301.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAGAATAGGATCATTCCA+AGG + Chr4:56478829-56478848 MsG0480021301.01.T01:CDS 35.0%
CTCGTGATAACAAGAAGAAT+AGG + Chr4:56478817-56478836 MsG0480021301.01.T01:CDS 35.0%
TCTTCTTAGGCAACAGAATT+GGG - Chr4:56478947-56478966 None:intergenic 35.0%
TTAGGAATGATGAAGAGCTT+GGG + Chr4:56478868-56478887 MsG0480021301.01.T01:CDS 35.0%
!! AACAGAATTGGGTTGATGTT+CGG - Chr4:56478936-56478955 None:intergenic 35.0%
AAGCACCAAGACAACAAAGA+AGG + Chr4:56478587-56478606 MsG0480021301.01.T01:CDS 40.0%
AGCACCAAGACAACAAAGAA+GGG + Chr4:56478588-56478607 MsG0480021301.01.T01:CDS 40.0%
GAGCATATCTACCTTTCTTC+AGG - Chr4:56478710-56478729 None:intergenic 40.0%
GATATTCAAGAACTGCTGCT+AGG - Chr4:56478761-56478780 None:intergenic 40.0%
GTCTTCTTAGGCAACAGAAT+TGG - Chr4:56478948-56478967 None:intergenic 40.0%
GTTAGGAATGATGAAGAGCT+TGG + Chr4:56478867-56478886 MsG0480021301.01.T01:CDS 40.0%
GTTGCCTAAGAAGACTGAAA+GGG + Chr4:56478953-56478972 MsG0480021301.01.T01:CDS 40.0%
TCTTGAATATCTTGCTGCTG+AGG + Chr4:56478770-56478789 MsG0480021301.01.T01:CDS 40.0%
TGTTGCCTAAGAAGACTGAA+AGG + Chr4:56478952-56478971 MsG0480021301.01.T01:CDS 40.0%
TTAGCCCTTTCAGTCTTCTT+AGG - Chr4:56478960-56478979 None:intergenic 40.0%
AAGAACACCACCATGAGCTA+TGG - Chr4:56478913-56478932 None:intergenic 45.0%
AAGACAACAAAGAAGGGAGC+CGG + Chr4:56478594-56478613 MsG0480021301.01.T01:CDS 45.0%
AAGACTGAAAGGGCTAACAC+TGG + Chr4:56478963-56478982 MsG0480021301.01.T01:CDS 45.0%
AGAACTGCTGCTAGGTAAAC+CGG - Chr4:56478753-56478772 None:intergenic 45.0%
ATCGGTCGATACCTGAAGAA+AGG + Chr4:56478696-56478715 MsG0480021301.01.T01:CDS 45.0%
CAATTCCCAGTCGGAAGAAT+CGG + Chr4:56478678-56478697 MsG0480021301.01.T01:CDS 45.0%
TGCTGCTGAGGTTCTTGAAT+TGG + Chr4:56478782-56478801 MsG0480021301.01.T01:CDS 45.0%
TGGTAGCAAGGAACCAAAGA+AGG + Chr4:56478983-56479002 MsG0480021301.01.T01:CDS 45.0%
! CATTCCAAGGCATTTGTTGC+TGG + Chr4:56478842-56478861 MsG0480021301.01.T01:CDS 45.0%
AAGGGCTAACACTGGTAGCA+AGG + Chr4:56478971-56478990 MsG0480021301.01.T01:CDS 50.0%
ACAGCCAGCAACAAATGCCT+TGG - Chr4:56478849-56478868 None:intergenic 50.0%
AGCAAGGAACCAAAGAAGGC+TGG + Chr4:56478987-56479006 MsG0480021301.01.T01:CDS 50.0%
AGGAGGTCCAAGGAAGAAGT+CGG + Chr4:56478629-56478648 MsG0480021301.01.T01:CDS 50.0%
CGATTCTTCCGACTGGGAAT+TGG - Chr4:56478680-56478699 None:intergenic 50.0%
GCTGAGGTTCTTGAATTGGC+AGG + Chr4:56478786-56478805 MsG0480021301.01.T01:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG - Chr4:56478999-56479018 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG + Chr4:56479004-56479023 MsG0480021301.01.T01:CDS 50.0%
GGTAGATATGCTCAGCGTGT+CGG + Chr4:56478717-56478736 MsG0480021301.01.T01:CDS 50.0%
GTGACAAGATCCATCAGAGC+CGG + Chr4:56478651-56478670 MsG0480021301.01.T01:CDS 50.0%
! ATCGACCGATTCTTCCGACT+GGG - Chr4:56478686-56478705 None:intergenic 50.0%
! GGCATTTGTTGCTGGCTGTT+AGG + Chr4:56478850-56478869 MsG0480021301.01.T01:CDS 50.0%
! GGCTCCCTTCTTTGTTGTCT+TGG - Chr4:56478595-56478614 None:intergenic 50.0%
! GGTGTTACCATAGCTCATGG+TGG + Chr4:56478903-56478922 MsG0480021301.01.T01:CDS 50.0%
! TATCGACCGATTCTTCCGAC+TGG - Chr4:56478687-56478706 None:intergenic 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG - Chr4:56478639-56478658 None:intergenic 50.0%
!! GCTGGTGTTACCATAGCTCA+TGG + Chr4:56478900-56478919 MsG0480021301.01.T01:CDS 50.0%
AAGGGAGCCGGTGGAAGAAA+GGG + Chr4:56478606-56478625 MsG0480021301.01.T01:CDS 55.0%
ACAACAAAGAAGGGAGCCGG+TGG + Chr4:56478597-56478616 MsG0480021301.01.T01:CDS 55.0%
ACCTCCTCCCTTTCTTCCAC+CGG - Chr4:56478616-56478635 None:intergenic 55.0%
GAAGAAAGGGAGGAGGTCCA+AGG + Chr4:56478619-56478638 MsG0480021301.01.T01:CDS 55.0%
GAGCTTGGGAAGTTGCTTGC+TGG + Chr4:56478882-56478901 MsG0480021301.01.T01:CDS 55.0%
TCCGACTGGGAATTGGAGAC+CGG - Chr4:56478673-56478692 None:intergenic 55.0%
TGCTAGGTAAACCGGAGCAC+CGG - Chr4:56478745-56478764 None:intergenic 55.0%
! GAATTGGAGACCGGCTCTGA+TGG - Chr4:56478664-56478683 None:intergenic 55.0%
GAAGGGAGCCGGTGGAAGAA+AGG + Chr4:56478605-56478624 MsG0480021301.01.T01:CDS 60.0%
GCCGGTCTCCAATTCCCAGT+CGG + Chr4:56478669-56478688 MsG0480021301.01.T01:CDS 60.0%
! TATGCTCAGCGTGTCGGCAC+CGG + Chr4:56478723-56478742 MsG0480021301.01.T01:CDS 60.0%
GCCGGTGGAAGAAAGGGAGG+AGG + Chr4:56478612-56478631 MsG0480021301.01.T01:CDS 65.0%
GGAGCCGGTGGAAGAAAGGG+AGG + Chr4:56478609-56478628 MsG0480021301.01.T01:CDS 65.0%
!! GCGTGTCGGCACCGGTGCTC+CGG + Chr4:56478731-56478750 MsG0480021301.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 56478579 56479031 56478579 ID=MsG0480021301.01;Name=MsG0480021301.01
Chr4 mRNA 56478579 56479031 56478579 ID=MsG0480021301.01.T01;Parent=MsG0480021301.01;Name=MsG0480021301.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|150
Chr4 exon 56478579 56479031 56478579 ID=MsG0480021301.01.T01:exon:19062;Parent=MsG0480021301.01.T01
Chr4 CDS 56478579 56479031 56478579 ID=MsG0480021301.01.T01:cds;Parent=MsG0480021301.01.T01
Gene Sequence

>MsG0480021301.01.T01

ATGGACGCAAGCACCAAGACAACAAAGAAGGGAGCCGGTGGAAGAAAGGGAGGAGGTCCAAGGAAGAAGTCGGTGACAAGATCCATCAGAGCCGGTCTCCAATTCCCAGTCGGAAGAATCGGTCGATACCTGAAGAAAGGTAGATATGCTCAGCGTGTCGGCACCGGTGCTCCGGTTTACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAGGTTCTTGAATTGGCAGGAAACGCTGCTCGTGATAACAAGAAGAATAGGATCATTCCAAGGCATTTGTTGCTGGCTGTTAGGAATGATGAAGAGCTTGGGAAGTTGCTTGCTGGTGTTACCATAGCTCATGGTGGTGTTCTTCCGAACATCAACCCAATTCTGTTGCCTAAGAAGACTGAAAGGGCTAACACTGGTAGCAAGGAACCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MsG0480021301.01.T01

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHLLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTERANTGSKEPKKAGKSPKKA*