AlfalfaGEDB | MsG0580028987.01

M. sativa cultivar ZhongmuNo.1 / MsG0580028987.01

KEGG: K09874
GO: GO:0015267 GO:0016020 GO:0055085
PFAM: PF00230

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028987.01.T01	AES99338.1	95.149	268	11	1	1	266	1	268	0	515

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028987.01.T01	P49173	65.267	262	84	2	4	258	3	264	7.98E-123	353

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028987.01.T01	G7KBN5	95.149	268	11	1	1	266	1	268	0.0	515

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003741.01	MsG0580028987.01	0.800591	1.341440e-48	6.270365e-46
MsG0580028987.01	MsG0580029310.01	0.821819	3.295780e-53	2.710418e-50
MsG0580028987.01	MsG0780041259.01	0.806161	9.408612e-50	5.070990e-47
MsG0580028987.01	MsG0880042958.01	0.831288	1.817119e-55	1.963015e-52
MsG0580028987.01	MsG0880042959.01	0.807045	6.123280e-50	3.375855e-47

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PPI

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Gene1	Gene2	Type
MsG0380011693.01	MsG0580028987.01	PPI
MsG0380015447.01	MsG0580028987.01	PPI
MsG0480018608.01	MsG0580028987.01	PPI
MsG0480020913.01	MsG0580028987.01	PPI
MsG0580024653.01	MsG0580028987.01	PPI
MsG0580028987.01	MsG0480019955.01	PPI
MsG0580029158.01	MsG0580028987.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028987.01.T01	MTR_5g079570	95.149	268	11	1	1	266	1	268	0.0	515
MsG0580028987.01.T01	MTR_3g008920	73.361	244	63	1	17	258	5	248	1.29e-132	375
MsG0580028987.01.T01	MTR_4g084880	58.233	249	97	2	11	252	4	252	6.66e-107	311
MsG0580028987.01.T01	MTR_8g087720	54.425	226	102	1	28	252	28	253	2.83e-86	258
MsG0580028987.01.T01	MTR_2g017590	50.000	252	117	3	7	252	3	251	6.69e-86	257
MsG0580028987.01.T01	MTR_2g017610	52.301	239	105	3	20	252	18	253	1.03e-84	254
MsG0580028987.01.T01	MTR_2g017660	47.191	267	133	4	3	264	5	268	4.93e-84	253
MsG0580028987.01.T01	MTR_2g017570	51.883	239	106	3	20	252	18	253	6.08e-84	252
MsG0580028987.01.T01	MTR_2g017620	53.043	230	100	3	31	255	17	243	1.54e-82	248
MsG0580028987.01.T01	MTR_2g017620	53.043	230	100	3	31	255	31	257	2.01e-82	248

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028987.01.T01	AT5G37820	58.915	258	100	2	1	252	1	258	6.62e-110	319
MsG0580028987.01.T01	AT5G37820	58.915	258	100	2	1	252	37	294	1.19e-109	319
MsG0580028987.01.T01	AT5G37810	56.977	258	105	2	1	252	1	258	4.72e-106	309
MsG0580028987.01.T01	AT4G18910	53.947	228	97	3	33	252	47	274	2.50e-83	251
MsG0580028987.01.T01	AT4G19030	47.126	261	128	5	1	252	18	277	3.96e-78	238
MsG0580028987.01.T01	AT1G31885	51.770	226	101	3	33	250	38	263	7.05e-77	236
MsG0580028987.01.T01	AT1G31885	53.110	209	90	3	50	250	1	209	8.70e-71	219
MsG0580028987.01.T01	AT2G34390	44.068	236	119	4	33	260	43	273	9.96e-67	209
MsG0580028987.01.T01	AT2G34390	43.966	232	117	4	39	262	7	233	8.81e-65	202
MsG0580028987.01.T01	AT4G18910	52.273	176	76	3	33	200	47	222	1.04e-57	184

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Find 64 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAAGTACCTCTTTAAG+TGG	0.467838	5:-89953863	None:intergenic
AAATAAGTACCTCTTTAAGT+GGG	0.550356	5:-89953862	None:intergenic
AACAAAGTTGCATGACGTGA+GGG	0.603296	5:-89952784	None:intergenic
AACGGTTCCAAAATAAGTTT+TGG	0.262047	5:-89954104	None:intergenic
AAGTGGGGTCCTACGAAAGA+TGG	0.521239	5:-89953846	None:intergenic
AATAAGTACCTCTTTAAGTG+GGG	0.663262	5:-89953861	None:intergenic
AATTAGCAGATGAACAATTC+AGG	0.402602	5:+89954848	MsG0580028987.01.T01:CDS
ACCCTGCTGTTACCATCACT+TGG	0.498343	5:+89953822	MsG0580028987.01.T01:CDS
ACCGTTCCCGTTGGATCAAA+TGG	0.412321	5:+89954121	MsG0580028987.01.T01:CDS
ACGAAAGATGGTCCAAGTGA+TGG	0.593945	5:-89953834	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAAGTACCTCTTTAAGTG+GGG	-	Chr5:89953864-89953883	None:intergenic	30.0%
AATGAGAGATTTAGCATGTT+TGG	-	Chr5:89953434-89953453	None:intergenic	30.0%
AATTAGCAGATGAACAATTC+AGG	+	Chr5:89954848-89954867	MsG0580028987.01.T01:CDS	30.0%
AGATTTAACGAGAGAGAATT+TGG	-	Chr5:89953296-89953315	None:intergenic	30.0%
CAACATTATAGTCATTCCAA+TGG	-	Chr5:89954529-89954548	None:intergenic	30.0%
CATATGTTTCTTGTACATGT+AGG	+	Chr5:89954003-89954022	MsG0580028987.01.T01:intron	30.0%
CTACATGTACAAGAAACATA+TGG	-	Chr5:89954005-89954024	None:intergenic	30.0%
GTAAGTTAGTGTGTCTTAAT+TGG	+	Chr5:89952842-89952861	MsG0580028987.01.T01:intron	30.0%
TAAACATGTACTATAAGTGC+AGG	-	Chr5:89954733-89954752	None:intergenic	30.0%
TAATCTTGTGGAAAATGACA+TGG	-	Chr5:89953521-89953540	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	89952725	89954888	89952725	ID=MsG0580028987.01;Name=MsG0580028987.01
Chr5	mRNA	89952725	89954888	89952725	ID=MsG0580028987.01.T01;Parent=MsG0580028987.01;Name=MsG0580028987.01.T01;_AED=0.44;_eAED=0.45;_QI=0\|0\|0\|1\|1\|1\|5\|0\|266
Chr5	exon	89952725	89952841	89952725	ID=MsG0580028987.01.T01:exon:17549;Parent=MsG0580028987.01.T01
Chr5	exon	89953653	89953874	89953653	ID=MsG0580028987.01.T01:exon:17550;Parent=MsG0580028987.01.T01
Chr5	exon	89954025	89954222	89954025	ID=MsG0580028987.01.T01:exon:17551;Parent=MsG0580028987.01.T01
Chr5	exon	89954504	89954565	89954504	ID=MsG0580028987.01.T01:exon:17552;Parent=MsG0580028987.01.T01
Chr5	exon	89954687	89954888	89954687	ID=MsG0580028987.01.T01:exon:17553;Parent=MsG0580028987.01.T01
Chr5	CDS	89952725	89952841	89952725	ID=MsG0580028987.01.T01:cds;Parent=MsG0580028987.01.T01
Chr5	CDS	89953653	89953874	89953653	ID=MsG0580028987.01.T01:cds;Parent=MsG0580028987.01.T01
Chr5	CDS	89954025	89954222	89954025	ID=MsG0580028987.01.T01:cds;Parent=MsG0580028987.01.T01
Chr5	CDS	89954504	89954565	89954504	ID=MsG0580028987.01.T01:cds;Parent=MsG0580028987.01.T01
Chr5	CDS	89954687	89954888	89954687	ID=MsG0580028987.01.T01:cds;Parent=MsG0580028987.01.T01

Gene Sequence

>MsG0580028987.01.T01

ATGGCTAACAAACGTGAAGACATTCATGACGATGAAATTTCAAGTGTTGAAGAAGGAAACCCTCACGTCATGCAACTTTGTTGTTCATCAAACAATACGATAACCTTGATACAGAAGGTGATTGCAGAAATCATTGGAACATATTTTGTGGTGTTTGCTGGATGTGGTTCTGTAGCGGTGGATAAGATCTATGGCTCTGTAACATTTCCAGGGGTATGTATCACTTGGGGGCTTATTGTAATGGTCATGTCTTACTCTGTTGGTCATGTCTCTGGAGGACACTTCAACCCTGCTGTTACCATCACTTGGACCATCTTTCGTAGGACCCCACTTAAAGAGGCTTCACTATACATTTTTGCTCAGTTGGTAGGATCAACGCTTGCAAGTGGAACATTAACTCTAATGTTTGATATCACATCCAAAACTTATTTTGGAACCGTTCCCGTTGGATCAAATGGCCAGTCTTTAGTTGCTGAAATCATTATCAGTTTTCTCTTAATGTTTGTCATTTCAGCGGTTTCAACTGACGAAAGAGCGGTGAATGATTTCGCCGGTGTTGCCATTGGAATGACTATAATGTTGAATGTCTTCATTGCTGGGCCTGTATCAGGAGGATCTATGAACCCAGCAAGAAGTATCGGTCCTGCACTTATAGTACATGTTTACAAAGGGTTATGGATATATGTGGTTGGTCCAATTGTTGGAGCTATAGTTGGAGCAATTGCATATAACTTTCTTAGATCCATAAAGTCACCTTCAGAATTAGCAGATGAACAATTCAGGAACATTGTTGTAGGTTGA

Protein sequence

>MsG0580028987.01.T01

MANKREDIHDDEISSVEEGNPHVMQLCCSSNNTITLIQKVIAEIIGTYFVVFAGCGSVAVDKIYGSVTFPGVCITWGLIVMVMSYSVGHVSGGHFNPAVTITWTIFRRTPLKEASLYIFAQLVGSTLASGTLTLMFDITSKTYFGTVPVGSNGQSLVAEIIISFLLMFVISAVSTDERAVNDFAGVAIGMTIMLNVFIAGPVSGGSMNPARSIGPALIVHVYKGLWIYVVGPIVGAIVGAIAYNFLRSIKSPSELADEQFRNIVVG*