AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042958.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042958.01.T02 MTR_8g027100 97.753 89 2 0 2 90 188 276 4.14e-56 174
MsG0880042958.01.T01 MTR_8g027100 97.753 89 2 0 2 90 188 276 4.14e-56 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATTGAAAAAATTAATT+TGG + Chr8:16689644-16689663 None:intergenic 10.0%
!!! AAATTAATTTTTTCAATTCA+TGG - Chr8:16689643-16689662 MsG0880042958.01.T02:CDS 10.0%
!!! ATCATATATTATGAAATTTT+TGG - Chr8:16689850-16689869 MsG0880042958.01.T02:intron 10.0%
!!! TATAAATTTTGTCAAAAAAA+TGG + Chr8:16689776-16689795 None:intergenic 10.0%
!!! TCATATATTATGAAATTTTT+GGG - Chr8:16689851-16689870 MsG0880042958.01.T02:intron 10.0%
!! ATTATTTGCTTGAAATTAAT+TGG - Chr8:16688438-16688457 MsG0880042958.01.T02:intron 15.0%
!! TTCAAGCAAATAATAATTAT+TGG + Chr8:16688432-16688451 None:intergenic 15.0%
!!! AAAAAATTAATTTGGTAAAC+AGG + Chr8:16689636-16689655 None:intergenic 15.0%
!!! CTAAATAATCATTTGATTTT+AGG - Chr8:16688851-16688870 MsG0880042958.01.T02:intron 15.0%
!!! GCAAATAAATTTATTTTGTT+TGG + Chr8:16689488-16689507 None:intergenic 15.0%
!!! TGTTTTTAAGATAAATAGAT+AGG - Chr8:16689121-16689140 MsG0880042958.01.T02:intron 15.0%
!! AAACTCTATAAAATCATGTT+TGG - Chr8:16689371-16689390 MsG0880042958.01.T02:intron 20.0%
!! AAGATAAATAGATAGGTATA+TGG - Chr8:16689128-16689147 MsG0880042958.01.T02:intron 20.0%
!! AATAATACTAAGTGAATGTT+TGG + Chr8:16689565-16689584 None:intergenic 20.0%
!! ATATTATCATTTACTTGTGA+TGG - Chr8:16688665-16688684 MsG0880042958.01.T02:intron 20.0%
!! CTATTTATCTTAAAAACAGA+AGG + Chr8:16689119-16689138 None:intergenic 20.0%
!! TAATTACATATATAGCCTTT+AGG + Chr8:16688212-16688231 None:intergenic 20.0%
!! TTAAACATCAACCATATATA+TGG + Chr8:16690113-16690132 None:intergenic 20.0%
!!! ATGAAATTTTTGGGTTTATT+TGG - Chr8:16689860-16689879 MsG0880042958.01.T02:intron 20.0%
!!! ATTTATTTCCATTTTCACAA+TGG - Chr8:16688316-16688335 MsG0880042958.01.T02:intron 20.0%
!!! CTGTATTATTCCAATTTTAT+CGG - Chr8:16688974-16688993 MsG0880042958.01.T02:intron 20.0%
!!! TATATGGTTGATGTTTAATT+TGG - Chr8:16690115-16690134 MsG0880042958.01.T02:CDS 20.0%
! AAAAAAGACAGTTCATGAAA+AGG + Chr8:16688645-16688664 None:intergenic 25.0%
! AAAAAGACAGTTCATGAAAA+GGG + Chr8:16688644-16688663 None:intergenic 25.0%
! ACACAAATTACAAAATCACA+TGG + Chr8:16690030-16690049 None:intergenic 25.0%
! ACGTATGAGATTAAATACTT+AGG - Chr8:16689970-16689989 MsG0880042958.01.T02:intron 25.0%
! ATCTATTCAAGTGATATTGA+TGG - Chr8:16689711-16689730 MsG0880042958.01.T02:intron 25.0%
! ATGCATGTCTCAAATTAATT+TGG - Chr8:16688724-16688743 MsG0880042958.01.T02:intron 25.0%
! ATTAATAACCACATGAATGA+AGG - Chr8:16688565-16688584 MsG0880042958.01.T02:intron 25.0%
! ATTAATTTATAGTCCTTTCG+AGG + Chr8:16689004-16689023 None:intergenic 25.0%
! CAAGAAAAAAAGAGTTTAAG+AGG + Chr8:16689246-16689265 None:intergenic 25.0%
! CCAAAACTTAAAGCAAAAAA+TGG - Chr8:16688088-16688107 MsG0880042958.01.T02:three_prime_UTR 25.0%
! CGATAAAATTGGAATAATAC+AGG + Chr8:16688976-16688995 None:intergenic 25.0%
! GTAAACTCTTAGATACTTTA+GGG - Chr8:16688873-16688892 MsG0880042958.01.T02:intron 25.0%
! TCAATATCACTTGAATAGAT+TGG + Chr8:16689711-16689730 None:intergenic 25.0%
! TCTAAAAAAACACTCAAACT+GGG - Chr8:16689219-16689238 MsG0880042958.01.T02:intron 25.0%
! TTGCTTGAAATTAATTGGTT+AGG - Chr8:16688443-16688462 MsG0880042958.01.T02:intron 25.0%
! TTTATCTTAAAAACAGAAGG+AGG + Chr8:16689116-16689135 None:intergenic 25.0%
! TTTATTTGGATTAACCTTTC+TGG - Chr8:16689874-16689893 MsG0880042958.01.T02:intron 25.0%
!! TTATAGAGTTTGAATGCTTT+TGG + Chr8:16689362-16689381 None:intergenic 25.0%
!!! CCATTTTTTGCTTTAAGTTT+TGG + Chr8:16688091-16688110 None:intergenic 25.0%
!!! CTCTTAAACTCTTTTTTTCT+TGG - Chr8:16689244-16689263 MsG0880042958.01.T02:intron 25.0%
!!! GAGTGTTGATTTTTTTACAA+AGG + Chr8:16689188-16689207 None:intergenic 25.0%
AATCACATGGTTCAATTCTT+TGG + Chr8:16690017-16690036 None:intergenic 30.0%
ACAGAAGGAGGATAAAATTA+AGG + Chr8:16689104-16689123 None:intergenic 30.0%
ACTTTCTTGATGTCTTTCAT+TGG + Chr8:16688163-16688182 None:intergenic 30.0%
ATAGATAGGTATATGGATGA+TGG - Chr8:16689135-16689154 MsG0880042958.01.T02:intron 30.0%
ATGTATCCTGCTTAATTGAT+AGG + Chr8:16689280-16689299 None:intergenic 30.0%
GGTAAACTCTTAGATACTTT+AGG - Chr8:16688872-16688891 MsG0880042958.01.T02:intron 30.0%
GTTATCTGTGACCATATATA+TGG - Chr8:16690099-16690118 MsG0880042958.01.T02:CDS 30.0%
TAACATCCTATCAATTAAGC+AGG - Chr8:16689271-16689290 MsG0880042958.01.T02:intron 30.0%
TACATACACAATTAAACTCG+AGG - Chr8:16689063-16689082 MsG0880042958.01.T02:intron 30.0%
! ATGATAACCTTTTCAACCTA+AGG + Chr8:16688787-16688806 None:intergenic 30.0%
! CTCTAAAAAAACACTCAAAC+TGG - Chr8:16689218-16689237 MsG0880042958.01.T02:intron 30.0%
! TAATATGCCTCTTTTTGTAG+TGG - Chr8:16689745-16689764 MsG0880042958.01.T02:intron 30.0%
! TATCATTTACTTGTGATGGA+AGG - Chr8:16688669-16688688 MsG0880042958.01.T02:intron 30.0%
!!! CAGTTTGAGTGTTTTTTTAG+AGG + Chr8:16689220-16689239 None:intergenic 30.0%
CATTGACACCATTGTGAAAA+TGG + Chr8:16688327-16688346 None:intergenic 35.0%
GAAGATTATTCCCCTTGTTA+AGG - Chr8:16688126-16688145 MsG0880042958.01.T02:three_prime_UTR 35.0%
GCTATATGAACCTCTTTGTT+TGG + Chr8:16689679-16689698 None:intergenic 35.0%
GGTTTAACCACTACAAAAAG+AGG + Chr8:16689755-16689774 None:intergenic 35.0%
TACATGACAACACATACCTT+AGG - Chr8:16688768-16688787 MsG0880042958.01.T02:intron 35.0%
TGATGAAGAAGATGCTAAAG+AGG - Chr8:16688491-16688510 MsG0880042958.01.T02:intron 35.0%
TGATTCTTGACATCAAGATC+TGG + Chr8:16688526-16688545 None:intergenic 35.0%
TTGTAGATAGGATAGTGTTG+AGG - Chr8:16688406-16688425 MsG0880042958.01.T02:intron 35.0%
TTTCAGTGAAGATAGATGCA+GGG - Chr8:16689603-16689622 MsG0880042958.01.T02:CDS 35.0%
! AACCTCTTTGTTTGGATGTT+TGG + Chr8:16689671-16689690 None:intergenic 35.0%
! ACCTCTTTGTTTGGATGTTT+GGG + Chr8:16689670-16689689 None:intergenic 35.0%
! ACGTGCATTTTCCTTAACAA+GGG + Chr8:16688140-16688159 None:intergenic 35.0%
! AGGTCTTTTATCGCAGTTTA+AGG - Chr8:16688598-16688617 MsG0880042958.01.T02:intron 35.0%
!! ACACATACCTTAGGTTGAAA+AGG - Chr8:16688777-16688796 MsG0880042958.01.T02:intron 35.0%
!! ACTTTAGGGTTTAGTCGTAT+CGG - Chr8:16688887-16688906 MsG0880042958.01.T02:intron 35.0%
!! TGTGTTCATGTTGTTTCTGT+TGG - Chr8:16690047-16690066 MsG0880042958.01.T02:CDS 35.0%
AACCACATGAATGAAGGCAA+TGG - Chr8:16688571-16688590 MsG0880042958.01.T02:intron 40.0%
ACTAGCTAGTGTTAAGCCAT+GGG - Chr8:16689909-16689928 MsG0880042958.01.T02:intron 40.0%
ACTTGACTCAACCAAGACTT+TGG + Chr8:16688062-16688081 None:intergenic 40.0%
ATCTACAAAAGAGTCCTCAC+TGG + Chr8:16688394-16688413 None:intergenic 40.0%
CTACATCATGCACTTCCTAA+AGG - Chr8:16688194-16688213 MsG0880042958.01.T02:three_prime_UTR 40.0%
CTTTCAGTGAAGATAGATGC+AGG - Chr8:16689602-16689621 MsG0880042958.01.T02:CDS 40.0%
TACTAGCTAGTGTTAAGCCA+TGG - Chr8:16689908-16689927 MsG0880042958.01.T02:intron 40.0%
TCCCAAACATCCAAACAAAG+AGG - Chr8:16689666-16689685 MsG0880042958.01.T02:CDS 40.0%
TCGAGGACATCCGATAAAAT+TGG + Chr8:16688987-16689006 None:intergenic 40.0%
TCTTGACATCAAGATCTGGA+TGG + Chr8:16688522-16688541 None:intergenic 40.0%
TTATTCTGCAGCCAAAGTCT+TGG - Chr8:16688048-16688067 MsG0880042958.01.T02:three_prime_UTR 40.0%
! CGTGCATTTTCCTTAACAAG+GGG + Chr8:16688139-16688158 None:intergenic 40.0%
! GACGTGCATTTTCCTTAACA+AGG + Chr8:16688141-16688160 None:intergenic 40.0%
! GTGAGGACTCTTTTGTAGAT+AGG - Chr8:16688394-16688413 MsG0880042958.01.T02:intron 40.0%
! TACTTGTGATGGAAGGTTTG+TGG - Chr8:16688676-16688695 MsG0880042958.01.T02:intron 40.0%
! TTTTATCGGATGTCCTCGAA+AGG - Chr8:16688988-16689007 MsG0880042958.01.T02:intron 40.0%
!! TAACCTTTCTGGTTTGTTGC+AGG - Chr8:16689885-16689904 MsG0880042958.01.T02:intron 40.0%
AGATAACAGTGACACTCCCA+TGG + Chr8:16689928-16689947 None:intergenic 45.0%
AGCCATTGCCTTCATTCATG+TGG + Chr8:16688576-16688595 None:intergenic 45.0%
GCTTCATAGTTTGACCAGTG+AGG - Chr8:16688377-16688396 MsG0880042958.01.T02:intron 45.0%
GTACCTGCAACAAACCAGAA+AGG + Chr8:16689891-16689910 None:intergenic 45.0%
TGAATGAAGGCAATGGCTCA+AGG - Chr8:16688578-16688597 MsG0880042958.01.T02:intron 45.0%
!! ATGGGAGTGTCACTGTTATC+TGG - Chr8:16689927-16689946 MsG0880042958.01.T02:intron 45.0%
Chromosome Type Strat End Strand Name
Chr8 gene 16688037 16690151 16688037 ID=MsG0880042958.01;Name=MsG0880042958.01
Chr8 mRNA 16688037 16690151 16688037 ID=MsG0880042958.01.T01;Parent=MsG0880042958.01;Name=MsG0880042958.01.T01;_AED=0.43;_eAED=0.43;_QI=0|1|1|1|0.5|0.2|5|246|90
Chr8 CDS 16690008 16690151 16690008 ID=MsG0880042958.01.T01:cds;Parent=MsG0880042958.01.T01
Chr8 CDS 16689570 16689698 16689570 ID=MsG0880042958.01.T01:cds;Parent=MsG0880042958.01.T01
Chr8 three_prime_UTR 16689569 16689569 16689569 ID=MsG0880042958.01.T01:three_prime_utr;Parent=MsG0880042958.01.T01
Chr8 three_prime_UTR 16688037 16688281 16688037 ID=MsG0880042958.01.T01:three_prime_utr;Parent=MsG0880042958.01.T01
Chr8 mRNA 16688037 16690151 16688037 ID=MsG0880042958.01.T02;Parent=MsG0880042958.01;Name=MsG0880042958.01.T02;_AED=0.43;_eAED=0.43;_QI=0|1|1|1|0.66|0.25|4|246|90
Chr8 CDS 16690008 16690151 16690008 ID=MsG0880042958.01.T02:cds;Parent=MsG0880042958.01.T02
Chr8 CDS 16689570 16689698 16689570 ID=MsG0880042958.01.T02:cds;Parent=MsG0880042958.01.T02
Chr8 three_prime_UTR 16689569 16689569 16689569 ID=MsG0880042958.01.T02:three_prime_utr;Parent=MsG0880042958.01.T02
Chr8 three_prime_UTR 16688037 16688281 16688037 ID=MsG0880042958.01.T02:three_prime_utr;Parent=MsG0880042958.01.T02
Gene Sequence

>MsG0880042958.01.T02

ATGAAACTTGATTATTCTGCAGCCAAAGTCTTGGTTGAGTCAAGTGAAAAACCAAAACTTAAAGCAAAAAATGGATTTTCTTTTGCTAAGAAGATTATTCCCCTTGTTAAGGAAAATGCACGTCCAATGAAAGACATCAAGAAACTGATGAAGAAGATGCTAAAGAGGAAAATCCATCCAGATCTTGATGTCAAGAATCACAAAGCAGAAGAAAACATTATTAATAACCACATGAATGAAGGCAATGGCTCAAGGTCTTTTATCGCAGTTTAA

>MsG0880042958.01.T01

ATGAAACTTGATTATTCTGCAGCCAAAGTCTTGGTTGAGTCAAGTGAAAAACCAAAACTTAAAGCAAAAAATGGATTTTCTTTTGCTAAGAAGATTATTCCCCTTGTTAAGGAAAATGCACGTCCAATGAAAGACATCAAGAAACTGATGAAGAAGATGCTAAAGAGGAAAATCCATCCAGATCTTGATGTCAAGAATCACAAAGCAGAAGAAAACATTATTAATAACCACATGAATGAAGGCAATGGCTCAAGGTCTTTTATCGCAGTTTAA

Protein sequence

>MsG0880042958.01.T02

MKLDYSAAKVLVESSEKPKLKAKNGFSFAKKIIPLVKENARPMKDIKKLMKKMLKRKIHPDLDVKNHKAEENIINNHMNEGNGSRSFIAV*

>MsG0880042958.01.T01

MKLDYSAAKVLVESSEKPKLKAKNGFSFAKKIIPLVKENARPMKDIKKLMKKMLKRKIHPDLDVKNHKAEENIINNHMNEGNGSRSFIAV*