AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029015.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029015.01.T01 MTR_5g080030 88.810 420 36 4 1 418 1 411 0.0 758
MsG0580029015.01.T01 MTR_5g095070 63.265 245 75 4 43 287 34 263 3.56e-95 287
MsG0580029015.01.T01 MTR_5g095070 57.895 133 46 3 243 366 140 271 1.47e-37 137
MsG0580029015.01.T01 MTR_1g084150 39.643 280 98 4 160 417 1 231 1.05e-50 171
MsG0580029015.01.T01 MTR_7g005970 25.430 291 158 7 69 304 67 353 6.26e-14 73.6
MsG0580029015.01.T01 MTR_4g015240 49.254 67 29 1 342 408 39 100 6.83e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029015.01.T01 AT3G13350 25.100 251 150 8 45 261 46 292 2.03e-11 65.1

Find 78 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGATCTTCAGTGCTCTTT+TGG 0.157665 5:-90346342 None:intergenic
CACTAGTTTATCTCAATTAA+TGG 0.164628 5:+90344997 MsG0580029015.01.T01:CDS
ATTCAAATCACCTGGGAAAA+AGG 0.184616 5:-90346500 None:intergenic
AAAGCTGATCATCAGTTTAA+AGG 0.190474 5:+90346101 MsG0580029015.01.T01:CDS
CCAGTTGCAGTTTGAGTTGC+AGG 0.228640 5:-90344769 None:intergenic
TCGTAAGCTCACTGAGCTTT+TGG 0.297529 5:+90343675 MsG0580029015.01.T01:CDS
TTATATCCACTCTGTCCTAT+TGG 0.357025 5:-90345711 None:intergenic
AGAGGAAAGCATGAAGATAA+AGG 0.358251 5:+90346297 MsG0580029015.01.T01:CDS
CGTTCGTTAGATTTGCCAAC+TGG 0.361646 5:+90346529 MsG0580029015.01.T01:CDS
CTCTCAGTGAGGATATAATA+TGG 0.364315 5:-90343646 None:intergenic
AGTTGTTTCTGCTCTAAAAC+TGG 0.365684 5:+90344657 MsG0580029015.01.T01:CDS
TTCATGCTTTCCTCTTCATA+TGG 0.373376 5:-90346289 None:intergenic
TTCACGCTTGCCTCTTCATA+TGG 0.385956 5:-90345957 None:intergenic
AGAGGCAAGCGTGAAGATAA+AGG 0.407513 5:+90345965 MsG0580029015.01.T01:CDS
GTTGTCAGACAAGGAGAAAC+AGG 0.408256 5:+90345830 MsG0580029015.01.T01:CDS
CCTCTGTCAAACTACTCTTT+TGG 0.416635 5:+90346421 MsG0580029015.01.T01:CDS
TTAATTTCATTAATGGAAGA+AGG 0.420089 5:+90343526 MsG0580029016.01.T01:intron
GACTACTCTAGTCAAATTAC+AGG 0.434649 5:+90345058 MsG0580029015.01.T01:CDS
CCTGCAACTCAAACTGCAAC+TGG 0.435997 5:+90344769 MsG0580029015.01.T01:CDS
TCGTGACCTCCAAGTAAAAC+AGG 0.439872 5:-90344224 None:intergenic
CGATAATCATTCAAATCACC+TGG 0.440231 5:-90346508 None:intergenic
TTGAGACATTGTACTTCTAC+AGG 0.444314 5:+90344743 MsG0580029015.01.T01:CDS
ACTGCAACTGGCAGCAATAC+AGG 0.446573 5:+90344781 MsG0580029015.01.T01:CDS
AGGTTGATGAAGAAAAGAAA+TGG 0.453133 5:+90344629 MsG0580029015.01.T01:intron
AGGAAAGGAATTAGTAGTGA+TGG 0.453771 5:+90343546 MsG0580029015.01.T01:CDS
AGAACAAAGAAAAGAAGAAA+CGG 0.457756 5:+90345679 MsG0580029015.01.T01:CDS
TACTTGGAGGTCACGAAAAG+AGG 0.458176 5:+90344231 MsG0580029015.01.T01:CDS
TAGTAGTCACCACACACAAT+AGG 0.458403 5:-90346382 None:intergenic
AAACGGAGAGGTGCTCCAAT+AGG 0.474962 5:+90345696 MsG0580029015.01.T01:CDS
TTCATTAATGGAAGAAGGAA+AGG 0.479210 5:+90343531 MsG0580029016.01.T01:intron
TATGCACTCTTCTGTCCCCT+TGG 0.481011 5:-90346044 None:intergenic
GGTTGATGAAGAAAAGAAAT+GGG 0.483399 5:+90344630 MsG0580029015.01.T01:intron
TTCATCAATGGAAGAAGCAA+AGG 0.491657 5:+90343603 MsG0580029015.01.T01:CDS
CTTTATAAGGTCCAGTTGAA+AGG 0.507012 5:+90344960 MsG0580029015.01.T01:intron
AGAAGCAAAGGAATTAGAGA+TGG 0.514965 5:+90343615 MsG0580029015.01.T01:CDS
AGTAAAACAGGTACAAGTCA+AGG 0.532503 5:-90344212 None:intergenic
AAATCACGGCTATTGATGCT+TGG 0.536227 5:+90346132 MsG0580029015.01.T01:CDS
TAGAGATGGCTATTGATGCT+TGG 0.538363 5:+90345802 MsG0580029015.01.T01:CDS
TCGACTTAAAGCTTATCATC+AGG 0.545141 5:+90345758 MsG0580029015.01.T01:CDS
CGACAACAAGTATAGTCACT+CGG 0.548231 5:-90346557 None:intergenic
GTCTAGCGCATTCATGCTTG+AGG 0.557923 5:-90346076 None:intergenic
GTTCGTTAGATTTGCCAACT+GGG 0.558967 5:+90346530 MsG0580029015.01.T01:CDS
AACATGAGATTGAATCCAGA+AGG 0.564550 5:-90343700 None:intergenic
GTTTCTGCTCTAAAACTGGA+AGG 0.575106 5:+90344661 MsG0580029015.01.T01:CDS
TTTCATGTAGCCATATGAAG+AGG 0.580743 5:+90345947 MsG0580029015.01.T01:intron
AGTCACTCGGGTTCCCCAGT+TGG 0.582592 5:-90346544 None:intergenic
ATATAATATATTCCTCAAGC+AGG 0.589592 5:+90345728 MsG0580029015.01.T01:CDS
GTCGAGCACACTCCTGCTTG+AGG 0.592986 5:-90345740 None:intergenic
GAGCACTGAAGATCTTGAGA+GGG 0.594444 5:+90346348 MsG0580029015.01.T01:CDS
AAAGAAAAGAAGAAACATAG+AGG 0.596397 5:+90346017 MsG0580029015.01.T01:CDS
AGAGCACTGAAGATCTTGAG+AGG 0.596789 5:+90346347 MsG0580029015.01.T01:CDS
GAAACATAGAGGTGTTCCAA+GGG 0.597227 5:+90346028 MsG0580029015.01.T01:CDS
AGAAACATAGAGGTGTTCCA+AGG 0.610697 5:+90346027 MsG0580029015.01.T01:CDS
GTAAAACAGGTACAAGTCAA+GGG 0.610799 5:-90344211 None:intergenic
TTTCATGTAGCCATATGAAG+AGG 0.611306 5:+90346279 MsG0580029015.01.T01:intron
TTCGTTAGATTTGCCAACTG+GGG 0.615840 5:+90346531 MsG0580029015.01.T01:CDS
CCAAAAGAGTAGTTTGACAG+AGG 0.617143 5:-90346421 None:intergenic
GCTTATCATCAGGACGTAGG+TGG 0.622815 5:+90345768 MsG0580029015.01.T01:CDS
GGAATTAGTAGTGATGGAGA+TGG 0.631774 5:+90343552 MsG0580029015.01.T01:CDS
TGGATATCAAGAATTTCAAG+TGG 0.632369 5:+90345638 MsG0580029015.01.T01:CDS
GACAACAAGTATAGTCACTC+GGG 0.642555 5:-90346556 None:intergenic
TTGGAGGTCACGAAAAGAGG+AGG 0.644365 5:+90344234 MsG0580029015.01.T01:CDS
GATAATCATTCAAATCACCT+GGG 0.650646 5:-90346507 None:intergenic
GGACGAGCAAGATTATCAGA+AGG 0.655330 5:+90345018 MsG0580029015.01.T01:CDS
TTGGAATAAGTTGTCAGACA+AGG 0.660735 5:+90345821 MsG0580029015.01.T01:CDS
AAAGCTCAGTGAGCTTACGA+TGG 0.661623 5:-90343672 None:intergenic
GTTGATGAAGAAAAGAAATG+GGG 0.667629 5:+90344631 MsG0580029015.01.T01:CDS
ACATGAGATTGAATCCAGAA+GGG 0.670630 5:-90343699 None:intergenic
AAAGAAAAGAAGAAACGGAG+AGG 0.672313 5:+90345684 MsG0580029015.01.T01:CDS
AATTTGACTAGAGTAGTCCT+CGG 0.673968 5:-90345053 None:intergenic
AAAGCTTATCATCAGGACGT+AGG 0.684875 5:+90345765 MsG0580029015.01.T01:CDS
GGTGCTCCAATAGGACAGAG+TGG 0.688534 5:+90345705 MsG0580029015.01.T01:CDS
CATTAATTGAGATAAACTAG+TGG 0.690114 5:-90344996 None:intergenic
GAAGGCAGCAAAGATATCCG+AGG 0.700751 5:+90345036 MsG0580029015.01.T01:CDS
GAAAAGAGGAGGTTATCACC+AGG 0.701758 5:+90344245 MsG0580029015.01.T01:CDS
AAACATAGAGGTGTTCCAAG+GGG 0.716007 5:+90346029 MsG0580029015.01.T01:CDS
GAAGAGAGACCTATTGTGTG+TGG 0.716478 5:+90346373 MsG0580029015.01.T01:CDS
TACGATGGAAGCTCTCAGTG+AGG 0.727693 5:-90343657 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATAAGAGTTTTAAATA+TGG + Chr5:90345485-90345504 MsG0580029015.01.T01:intron 10.0%
!! TCATAAAAAAAATACAACTT+TGG + Chr5:90345210-90345229 MsG0580029015.01.T01:intron 15.0%
!! TTGAACAAATAAAACATATT+TGG - Chr5:90344546-90344565 None:intergenic 15.0%
!! ACAAATAAAACATATTTGGA+TGG - Chr5:90344542-90344561 None:intergenic 20.0%
!! ACATGAAAAGTAATTAGAAA+TGG - Chr5:90345935-90345954 None:intergenic 20.0%
!! ACCTTAAATTCTTCTTTATA+AGG + Chr5:90344947-90344966 MsG0580029015.01.T01:intron 20.0%
!! ACCTTATAAAGAAGAATTTA+AGG - Chr5:90344951-90344970 None:intergenic 20.0%
!!! AAATGAGATTTTTTCATCAA+TGG + Chr5:90343591-90343610 MsG0580029015.01.T01:CDS 20.0%
!!! ACAATCCAAATTTAGTTTAA+AGG + Chr5:90345579-90345598 MsG0580029015.01.T01:intron 20.0%
!!! AGATATACAATTTTAATGAG+TGG + Chr5:90345087-90345106 MsG0580029015.01.T01:intron 20.0%
!!! AGTTTATAATTTTACTTGAC+TGG + Chr5:90344834-90344853 MsG0580029015.01.T01:intron 20.0%
!!! ATCTCATTTTCATTTTCATA+AGG - Chr5:90343580-90343599 None:intergenic 20.0%
!!! ATTATGTTTATGTTGCATTT+TGG + Chr5:90344899-90344918 MsG0580029015.01.T01:intron 20.0%
!!! ATTCTTTTTCATTCAATGAA+TGG - Chr5:90345123-90345142 None:intergenic 20.0%
!!! TATGATACTTATTTTGAATC+AGG - Chr5:90345195-90345214 None:intergenic 20.0%
!!! TTTCTTCTTTTCTTTGTTAT+TGG - Chr5:90346012-90346031 None:intergenic 20.0%
! AAAGAAAAGAAGAAACATAG+AGG + Chr5:90346017-90346036 MsG0580029015.01.T01:CDS 25.0%
! AAATACATATAGTAGCAAAC+AGG - Chr5:90345300-90345319 None:intergenic 25.0%
! AATGAGAAAGAGATTAAAGA+AGG - Chr5:90343741-90343760 None:intergenic 25.0%
! ACATGAAATGTAATTACAAG+TGG - Chr5:90346267-90346286 None:intergenic 25.0%
! AGAACAAAGAAAAGAAGAAA+CGG + Chr5:90345679-90345698 MsG0580029015.01.T01:CDS 25.0%
! ATATAACCAAACAATATACC+TGG - Chr5:90344266-90344285 None:intergenic 25.0%
! ATATAATATATTCCTCAAGC+AGG + Chr5:90345728-90345747 MsG0580029015.01.T01:CDS 25.0%
! CATATTCGAAAATTCATACA+AGG - Chr5:90344579-90344598 None:intergenic 25.0%
! CATTAATTGAGATAAACTAG+TGG - Chr5:90344999-90345018 None:intergenic 25.0%
! GTAAAACAACCTTATACTTT+CGG + Chr5:90344075-90344094 MsG0580029015.01.T01:intron 25.0%
! TGTATTTATTATCTGCACTA+TGG + Chr5:90345313-90345332 MsG0580029015.01.T01:intron 25.0%
! TTCATATTCATTAATTGACG+TGG - Chr5:90344012-90344031 None:intergenic 25.0%
!! ATCCAAATTTAGTTTAAAGG+TGG + Chr5:90345582-90345601 MsG0580029015.01.T01:intron 25.0%
!! ATTTGTTGGAAGAGTTTAAA+GGG + Chr5:90345363-90345382 MsG0580029015.01.T01:intron 25.0%
!! CACTAGTTTATCTCAATTAA+TGG + Chr5:90344997-90345016 MsG0580029015.01.T01:CDS 25.0%
!! TATAATTTTACTTGACTGGT+TGG + Chr5:90344838-90344857 MsG0580029015.01.T01:intron 25.0%
!! TTAGTAACTCAATGATTTGT+TGG + Chr5:90345349-90345368 MsG0580029015.01.T01:intron 25.0%
!!! AATGGAAGCTACATATTTTT+TGG - Chr5:90344503-90344522 None:intergenic 25.0%
!!! AATGTCTTTTTCTAGTCTAT+TGG + Chr5:90343876-90343895 MsG0580029015.01.T01:intron 25.0%
!!! TTCTTTTCTTTGTTATTGGT+GGG - Chr5:90346008-90346027 None:intergenic 25.0%
AAAGCTGATCATCAGTTTAA+AGG + Chr5:90346101-90346120 MsG0580029015.01.T01:CDS 30.0%
AGCCACCTTTAAACTAAATT+TGG - Chr5:90345587-90345606 None:intergenic 30.0%
CACAATTGATCAATTCACTA+TGG + Chr5:90345241-90345260 MsG0580029015.01.T01:intron 30.0%
CCAAATAGAATTGGAAAAAG+AGG - Chr5:90344168-90344187 None:intergenic 30.0%
GAACATGCAAACGAAAAAAA+TGG - Chr5:90343775-90343794 None:intergenic 30.0%
GATAATCATTCAAATCACCT+GGG - Chr5:90346510-90346529 None:intergenic 30.0%
GTCATATTCATTAATTGACG+TGG + Chr5:90344042-90344061 MsG0580029015.01.T01:intron 30.0%
TAAAGAGAATGACATGCAAA+CGG - Chr5:90343818-90343837 None:intergenic 30.0%
TCAAAATCTGCAAAGAAATC+TGG - Chr5:90345450-90345469 None:intergenic 30.0%
TCAAATCTGCCAAATAGAAT+TGG - Chr5:90344177-90344196 None:intergenic 30.0%
TGGAAGAAAAATCATAGAGA+TGG + Chr5:90345788-90345807 MsG0580029015.01.T01:CDS 30.0%
TGGATATCAAGAATTTCAAG+TGG + Chr5:90345638-90345657 MsG0580029015.01.T01:CDS 30.0%
TTATATCCACATTGTTGACA+TGG + Chr5:90344280-90344299 MsG0580029015.01.T01:intron 30.0%
TTATCACCAGGTATATTGTT+TGG + Chr5:90344257-90344276 MsG0580029015.01.T01:intron 30.0%
! ATATAGTAGCAAACAGGTTT+TGG - Chr5:90345294-90345313 None:intergenic 30.0%
! CCTCTTTTTCCAATTCTATT+TGG + Chr5:90344165-90344184 MsG0580029015.01.T01:intron 30.0%
! CTTCTTTTCTTTGTTCTTTG+CGG - Chr5:90345676-90345695 None:intergenic 30.0%
! GTTGATGAAGAAAAGAAATG+GGG + Chr5:90344631-90344650 MsG0580029015.01.T01:CDS 30.0%
! TCTAGCAAAAAAGTTAGTCT+CGG + Chr5:90344387-90344406 MsG0580029015.01.T01:intron 30.0%
! TTCTTTTCTTTGTTCTTTGC+GGG - Chr5:90345675-90345694 None:intergenic 30.0%
!! AGGTTGATGAAGAAAAGAAA+TGG + Chr5:90344629-90344648 MsG0580029015.01.T01:intron 30.0%
!! GATTTGTTGGAAGAGTTTAA+AGG + Chr5:90345362-90345381 MsG0580029015.01.T01:intron 30.0%
!! GGTTGATGAAGAAAAGAAAT+GGG + Chr5:90344630-90344649 MsG0580029015.01.T01:intron 30.0%
!!! CTTCTTTTCTTTGTTATTGG+TGG - Chr5:90346009-90346028 None:intergenic 30.0%
!!! CTTTTCAAGTTGATCACATA+AGG - Chr5:90344698-90344717 None:intergenic 30.0%
AAAATCAACCATATCGTTGG+TGG + Chr5:90345405-90345424 MsG0580029015.01.T01:intron 35.0%
AAAGAAAAGAAGAAACGGAG+AGG + Chr5:90345684-90345703 MsG0580029015.01.T01:CDS 35.0%
AACATGAGATTGAATCCAGA+AGG - Chr5:90343703-90343722 None:intergenic 35.0%
AATTTGACTAGAGTAGTCCT+CGG - Chr5:90345056-90345075 None:intergenic 35.0%
ACAATACCATGTCAACAATG+TGG - Chr5:90344289-90344308 None:intergenic 35.0%
ACATGAGATTGAATCCAGAA+GGG - Chr5:90343702-90343721 None:intergenic 35.0%
AGAAGCAAAGGAATTAGAGA+TGG + Chr5:90343615-90343634 MsG0580029015.01.T01:CDS 35.0%
AGGAAAGGAATTAGTAGTGA+TGG + Chr5:90343546-90343565 MsG0580029015.01.T01:CDS 35.0%
AGTAAAACAGGTACAAGTCA+AGG - Chr5:90344215-90344234 None:intergenic 35.0%
ATTCAAATCACCTGGGAAAA+AGG - Chr5:90346503-90346522 None:intergenic 35.0%
CGATAATCATTCAAATCACC+TGG - Chr5:90346511-90346530 None:intergenic 35.0%
CTCTCAGTGAGGATATAATA+TGG - Chr5:90343649-90343668 None:intergenic 35.0%
CTTTATAAGGTCCAGTTGAA+AGG + Chr5:90344960-90344979 MsG0580029015.01.T01:intron 35.0%
GACTACTCTAGTCAAATTAC+AGG + Chr5:90345058-90345077 MsG0580029015.01.T01:CDS 35.0%
GATCAATTCACTATGGTCTA+TGG + Chr5:90345248-90345267 MsG0580029015.01.T01:intron 35.0%
GTAAAACAGGTACAAGTCAA+GGG - Chr5:90344214-90344233 None:intergenic 35.0%
TAAAGGTGACCGAAAAATCA+CGG + Chr5:90346118-90346137 MsG0580029015.01.T01:CDS 35.0%
TATGTAGCTTCTAACCAACA+AGG + Chr5:90344358-90344377 MsG0580029015.01.T01:intron 35.0%
TCGACTTAAAGCTTATCATC+AGG + Chr5:90345758-90345777 MsG0580029015.01.T01:CDS 35.0%
TGGAAGAGTTTAAAGGGATT+TGG + Chr5:90345369-90345388 MsG0580029015.01.T01:intron 35.0%
TTATATCCACTCTGTCCTAT+TGG - Chr5:90345714-90345733 None:intergenic 35.0%
TTCAAATCACCTGGGAAAAA+GGG - Chr5:90346502-90346521 None:intergenic 35.0%
TTCATCAATGGAAGAAGCAA+AGG + Chr5:90343603-90343622 MsG0580029015.01.T01:CDS 35.0%
TTCATGCTTTCCTCTTCATA+TGG - Chr5:90346292-90346311 None:intergenic 35.0%
TTGAGACATTGTACTTCTAC+AGG + Chr5:90344743-90344762 MsG0580029015.01.T01:CDS 35.0%
TTGCTAGATTCAAACCTTGT+TGG - Chr5:90344375-90344394 None:intergenic 35.0%
TTGGAATAAGTTGTCAGACA+AGG + Chr5:90345821-90345840 MsG0580029015.01.T01:CDS 35.0%
TTTCATGTAGCCATATGAAG+AGG + Chr5:90345947-90345966 MsG0580029015.01.T01:intron 35.0%
! AGAGGAAAGCATGAAGATAA+AGG + Chr5:90346297-90346316 MsG0580029015.01.T01:CDS 35.0%
! CATCAATAGCCGTGATTTTT+CGG - Chr5:90346130-90346149 None:intergenic 35.0%
! TAGCATACACCGAAAGTATA+AGG - Chr5:90344087-90344106 None:intergenic 35.0%
! TATCGTACCAGTTTTTCCAT+GGG - Chr5:90346170-90346189 None:intergenic 35.0%
! TTATCGTACCAGTTTTTCCA+TGG - Chr5:90346171-90346190 None:intergenic 35.0%
! TTCGTTTGCATGTTCTGTTT+TGG + Chr5:90343780-90343799 MsG0580029015.01.T01:intron 35.0%
!! ACCAACGATATGGTTGATTT+TGG - Chr5:90345406-90345425 None:intergenic 35.0%
!! AGTTGTTTCTGCTCTAAAAC+TGG + Chr5:90344657-90344676 MsG0580029015.01.T01:CDS 35.0%
!! TGACTTGTACCTGTTTTACT+TGG + Chr5:90344215-90344234 MsG0580029015.01.T01:CDS 35.0%
!!! GAGTTTTGATTCCTTTCAAC+TGG - Chr5:90344974-90344993 None:intergenic 35.0%
!!! TTTTTTGGTTTCCCACATAC+AGG - Chr5:90344488-90344507 None:intergenic 35.0%
AAAGCTTATCATCAGGACGT+AGG + Chr5:90345765-90345784 MsG0580029015.01.T01:CDS 40.0%
AAATCACGGCTATTGATGCT+TGG + Chr5:90346132-90346151 MsG0580029015.01.T01:CDS 40.0%
ACTGACAGAAAAAACATCCG+AGG + Chr5:90346474-90346493 MsG0580029015.01.T01:CDS 40.0%
CCAAAAGAGTAGTTTGACAG+AGG - Chr5:90346424-90346443 None:intergenic 40.0%
CTTTCAAACCACCAACGATA+TGG - Chr5:90345416-90345435 None:intergenic 40.0%
GCCAAAATCAACCATATCGT+TGG + Chr5:90345402-90345421 MsG0580029015.01.T01:intron 40.0%
GCTACGTCGAAAACTAAATG+TGG - Chr5:90344448-90344467 None:intergenic 40.0%
GGATAAGCTTGATCTGCAAA+TGG - Chr5:90344521-90344540 None:intergenic 40.0%
GTTCGTTAGATTTGCCAACT+GGG + Chr5:90346530-90346549 MsG0580029015.01.T01:CDS 40.0%
TAGAGATGGCTATTGATGCT+TGG + Chr5:90345802-90345821 MsG0580029015.01.T01:CDS 40.0%
TAGTAGTCACCACACACAAT+AGG - Chr5:90346385-90346404 None:intergenic 40.0%
TGGCAGCAATACAGGTTAAT+TGG + Chr5:90344789-90344808 MsG0580029015.01.T01:intron 40.0%
TTCGTTAGATTTGCCAACTG+GGG + Chr5:90346531-90346550 MsG0580029015.01.T01:CDS 40.0%
TTCTCTTGAATGATTGCTGC+AGG + Chr5:90344609-90344628 MsG0580029015.01.T01:intron 40.0%
! ATCGTACCAGTTTTTCCATG+GGG - Chr5:90346169-90346188 None:intergenic 40.0%
! CAAGATCTTCAGTGCTCTTT+TGG - Chr5:90346345-90346364 None:intergenic 40.0%
! CCTCTGTCAAACTACTCTTT+TGG + Chr5:90346421-90346440 MsG0580029015.01.T01:CDS 40.0%
! CTTGTACCTGTTTTACTTGG+AGG + Chr5:90344218-90344237 MsG0580029015.01.T01:CDS 40.0%
! GGAATTAGTAGTGATGGAGA+TGG + Chr5:90343552-90343571 MsG0580029015.01.T01:CDS 40.0%
!! AAACATAGAGGTGTTCCAAG+GGG + Chr5:90346029-90346048 MsG0580029015.01.T01:CDS 40.0%
!! AGAAACATAGAGGTGTTCCA+AGG + Chr5:90346027-90346046 MsG0580029015.01.T01:CDS 40.0%
!! GAAACATAGAGGTGTTCCAA+GGG + Chr5:90346028-90346047 MsG0580029015.01.T01:CDS 40.0%
!! GTTTCTGCTCTAAAACTGGA+AGG + Chr5:90344661-90344680 MsG0580029015.01.T01:CDS 40.0%
AAAGCTCAGTGAGCTTACGA+TGG - Chr5:90343675-90343694 None:intergenic 45.0%
ACAAGGTTTCCTCCTGTATG+TGG + Chr5:90344473-90344492 MsG0580029015.01.T01:intron 45.0%
AGAGGCAAGCGTGAAGATAA+AGG + Chr5:90345965-90345984 MsG0580029015.01.T01:CDS 45.0%
CAAGGTTTCCTCCTGTATGT+GGG + Chr5:90344474-90344493 MsG0580029015.01.T01:intron 45.0%
CGTTCGTTAGATTTGCCAAC+TGG + Chr5:90346529-90346548 MsG0580029015.01.T01:CDS 45.0%
CTCTACGTAGTCAACCACAT+CGG - Chr5:90345537-90345556 None:intergenic 45.0%
CTGCAAAGAAATCTGGACTG+CGG - Chr5:90345443-90345462 None:intergenic 45.0%
GAAAAGAGGAGGTTATCACC+AGG + Chr5:90344245-90344264 MsG0580029015.01.T01:CDS 45.0%
GAAGAGAGACCTATTGTGTG+TGG + Chr5:90346373-90346392 MsG0580029015.01.T01:CDS 45.0%
GGACGAGCAAGATTATCAGA+AGG + Chr5:90345018-90345037 MsG0580029015.01.T01:CDS 45.0%
GGTGGCTCATATGAATTCAG+AGG + Chr5:90345600-90345619 MsG0580029015.01.T01:intron 45.0%
GTTGACTACGTAGAGTCACA+CGG + Chr5:90345542-90345561 MsG0580029015.01.T01:intron 45.0%
GTTGTCAGACAAGGAGAAAC+AGG + Chr5:90345830-90345849 MsG0580029015.01.T01:CDS 45.0%
TACTTGGAGGTCACGAAAAG+AGG + Chr5:90344231-90344250 MsG0580029015.01.T01:CDS 45.0%
TCGTGACCTCCAAGTAAAAC+AGG - Chr5:90344227-90344246 None:intergenic 45.0%
TTCACGCTTGCCTCTTCATA+TGG - Chr5:90345960-90345979 None:intergenic 45.0%
TTGACTACGTAGAGTCACAC+GGG + Chr5:90345543-90345562 MsG0580029015.01.T01:intron 45.0%
TTTCGACGTAGCCTCAAACA+AGG + Chr5:90344456-90344475 MsG0580029015.01.T01:intron 45.0%
! ACAGGAGGAAACCTTGTTTG+AGG - Chr5:90344470-90344489 None:intergenic 45.0%
! TCGTAAGCTCACTGAGCTTT+TGG + Chr5:90343675-90343694 MsG0580029015.01.T01:CDS 45.0%
! TCGTACCAGTTTTTCCATGG+GGG - Chr5:90346168-90346187 None:intergenic 45.0%
! TTTGGTTTCCCACATACAGG+AGG - Chr5:90344485-90344504 None:intergenic 45.0%
!! AGAGCACTGAAGATCTTGAG+AGG + Chr5:90346347-90346366 MsG0580029015.01.T01:CDS 45.0%
!! GAATTCAGAGGTGCTTATGC+AGG + Chr5:90345612-90345631 MsG0580029015.01.T01:intron 45.0%
!! GAGCACTGAAGATCTTGAGA+GGG + Chr5:90346348-90346367 MsG0580029015.01.T01:CDS 45.0%
!!! ATTAAACTAATTTTTTTTTA+TGG + Chr5:90344107-90344126 MsG0580029015.01.T01:intron 5.0%
ACCTGGGAAAAAGGGATCCT+CGG - Chr5:90346494-90346513 None:intergenic 50.0%
ACTGCAACTGGCAGCAATAC+AGG + Chr5:90344781-90344800 MsG0580029015.01.T01:CDS 50.0%
CCTGCAACTCAAACTGCAAC+TGG + Chr5:90344769-90344788 MsG0580029015.01.T01:CDS 50.0%
GAAGGCAGCAAAGATATCCG+AGG + Chr5:90345036-90345055 MsG0580029015.01.T01:CDS 50.0%
GATGTCCCCCATGGAAAAAC+TGG + Chr5:90346160-90346179 MsG0580029015.01.T01:CDS 50.0%
GCTTATCATCAGGACGTAGG+TGG + Chr5:90345768-90345787 MsG0580029015.01.T01:CDS 50.0%
GTCTAGCGCATTCATGCTTG+AGG - Chr5:90346079-90346098 None:intergenic 50.0%
GTGGTTCGATCAAGATGGGT+TGG - Chr5:90345512-90345531 None:intergenic 50.0%
TACGATGGAAGCTCTCAGTG+AGG - Chr5:90343660-90343679 None:intergenic 50.0%
TACGTAGTCAACCACATCGG+TGG - Chr5:90345534-90345553 None:intergenic 50.0%
TATGCACTCTTCTGTCCCCT+TGG - Chr5:90346047-90346066 None:intergenic 50.0%
TTGGAAGATGATGTCCCCCA+TGG + Chr5:90346151-90346170 MsG0580029015.01.T01:CDS 50.0%
TTGGAGGTCACGAAAAGAGG+AGG + Chr5:90344234-90344253 MsG0580029015.01.T01:CDS 50.0%
! ACTGAGCTTTTGGACCCTTC+TGG + Chr5:90343685-90343704 MsG0580029015.01.T01:CDS 50.0%
! CTCGAATCTCCTCTGAGAGT+GGG + Chr5:90344420-90344439 MsG0580029015.01.T01:intron 50.0%
! CTCTTTTGGCAACAACGCAG+TGG + Chr5:90346435-90346454 MsG0580029015.01.T01:CDS 50.0%
! GGTGGTGGTTCGATCAAGAT+GGG - Chr5:90345516-90345535 None:intergenic 50.0%
! TCGAATCTCCTCTGAGAGTG+GGG + Chr5:90344421-90344440 MsG0580029015.01.T01:intron 50.0%
! TTTTGGCAACAACGCAGTGG+TGG + Chr5:90346438-90346457 MsG0580029015.01.T01:CDS 50.0%
!! AAACGGAGAGGTGCTCCAAT+AGG + Chr5:90345696-90345715 MsG0580029015.01.T01:CDS 50.0%
!! AGAGGTGCTTATGCAGGAGT+TGG + Chr5:90345618-90345637 MsG0580029015.01.T01:intron 50.0%
!! CCAGTTGCAGTTTGAGTTGC+AGG - Chr5:90344772-90344791 None:intergenic 50.0%
AATGTGGACCCCACTCTCAG+AGG - Chr5:90344432-90344451 None:intergenic 55.0%
GGTGCTCCAATAGGACAGAG+TGG + Chr5:90345705-90345724 MsG0580029015.01.T01:CDS 55.0%
GTAGTCAACCACATCGGTGG+TGG - Chr5:90345531-90345550 None:intergenic 55.0%
TGATCGAACCACCACCGATG+TGG + Chr5:90345520-90345539 MsG0580029015.01.T01:intron 55.0%
! CGGTGGTGGTTCGATCAAGA+TGG - Chr5:90345517-90345536 None:intergenic 55.0%
! GCTCGAATCTCCTCTGAGAG+TGG + Chr5:90344419-90344438 MsG0580029015.01.T01:intron 55.0%
! TCCGAGGATCCCTTTTTCCC+AGG + Chr5:90346490-90346509 MsG0580029015.01.T01:CDS 55.0%
GTCGAGCACACTCCTGCTTG+AGG - Chr5:90345743-90345762 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 90343538 90346564 90343538 ID=MsG0580029015.01;Name=MsG0580029015.01
Chr5 mRNA 90343538 90346564 90343538 ID=MsG0580029015.01.T01;Parent=MsG0580029015.01;Name=MsG0580029015.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|7|0|419
Chr5 exon 90343538 90343719 90343538 ID=MsG0580029015.01.T01:exon:26131;Parent=MsG0580029015.01.T01
Chr5 exon 90344191 90344266 90344191 ID=MsG0580029015.01.T01:exon:26132;Parent=MsG0580029015.01.T01
Chr5 exon 90344631 90344802 90344631 ID=MsG0580029015.01.T01:exon:26133;Parent=MsG0580029015.01.T01
Chr5 exon 90344969 90345079 90344969 ID=MsG0580029015.01.T01:exon:26134;Parent=MsG0580029015.01.T01
Chr5 exon 90345634 90345851 90345634 ID=MsG0580029015.01.T01:exon:26135;Parent=MsG0580029015.01.T01
Chr5 exon 90345957 90346181 90345957 ID=MsG0580029015.01.T01:exon:26136;Parent=MsG0580029015.01.T01
Chr5 exon 90346289 90346564 90346289 ID=MsG0580029015.01.T01:exon:26137;Parent=MsG0580029015.01.T01
Chr5 CDS 90343538 90343719 90343538 ID=MsG0580029015.01.T01:cds;Parent=MsG0580029015.01.T01
Chr5 CDS 90344191 90344266 90344191 ID=MsG0580029015.01.T01:cds;Parent=MsG0580029015.01.T01
Chr5 CDS 90344631 90344802 90344631 ID=MsG0580029015.01.T01:cds;Parent=MsG0580029015.01.T01
Chr5 CDS 90344969 90345079 90344969 ID=MsG0580029015.01.T01:cds;Parent=MsG0580029015.01.T01
Chr5 CDS 90345634 90345851 90345634 ID=MsG0580029015.01.T01:cds;Parent=MsG0580029015.01.T01
Chr5 CDS 90345957 90346181 90345957 ID=MsG0580029015.01.T01:cds;Parent=MsG0580029015.01.T01
Chr5 CDS 90346289 90346564 90346289 ID=MsG0580029015.01.T01:cds;Parent=MsG0580029015.01.T01
Gene Sequence

>MsG0580029015.01.T01

ATGGAAGAAGGAAAGGAATTAGTAGTGATGGAGATGGTTCCTTATGAAAATGAAAATGAGATTTTTTCATCAATGGAAGAAGCAAAGGAATTAGAGATGGAGATGATTCCATATTATATCCTCACTGAGAGCTTCCATCGTAAGCTCACTGAGCTTTTGGACCCTTCTGGATTCAATCTCATATTTGATGTTCGAAAAACATCCCTTGACTTGTACCTGTTTTACTTGGAGGTCACGAAAAGAGGAGGTTATCACCAGGTTGATGAAGAAAAGAAATGGGGTGAAGTTGTTTCTGCTCTAAAACTGGAAGGAAACAATGCAACCTTATGTGATCAACTTGAAAAGCTATATAAAGAGCTTCTGTACAAATTTGAGACATTGTACTTCTACAGGTCTCCTGCAACTCAAACTGCAACTGGCAGCAATACAGGTCCAGTTGAAAGGAATCAAAACTCAACCACTAGTTTATCTCAATTAATGGACGAGCAAGATTATCAGAAGGCAGCAAAGATATCCGAGGACTACTCTAGTCAAATTACAGGAGTTGGATATCAAGAATTTCAAGTGGTTCAACAAGCACCCGCAAAGAACAAAGAAAAGAAGAAACGGAGAGGTGCTCCAATAGGACAGAGTGGATATAATATATTCCTCAAGCAGGAGTGTGCTCGACTTAAAGCTTATCATCAGGACGTAGGTGGAAGAAAAATCATAGAGATGGCTATTGATGCTTGGAATAAGTTGTCAGACAAGGAGAAACAGCCATATGAAGAGGCAAGCGTGAAGATAAAGGAAGAAATTAAAGAAGCACCCACCAATAACAAAGAAAAGAAGAAACATAGAGGTGTTCCAAGGGGACAGAAGAGTGCATATCAAATATTCCTCAAGCATGAATGCGCTAGACTTAAAGCTGATCATCAGTTTAAAGGTGACCGAAAAATCACGGCTATTGATGCTTGGAAGATGATGTCCCCCATGGAAAAACTGCCATATGAAGAGGAAAGCATGAAGATAAAGGAAGAAATTAAAGAAGCAATGATCCAAAAGAGCACTGAAGATCTTGAGAGGGATGAAGAGAGACCTATTGTGTGTGGTGACTACTACTCTGTAACTTCACAACCTCTGTCAAACTACTCTTTTGGCAACAACGCAGTGGTGGACTTAGATTTTAAACTGACAGAAAAAACATCCGAGGATCCCTTTTTCCCAGGTGATTTGAATGATTATCGTTCGTTAGATTTGCCAACTGGGGAACCCGAGTGA
Protein sequence

>MsG0580029015.01.T01

MEEGKELVVMEMVPYENENEIFSSMEEAKELEMEMIPYYILTESFHRKLTELLDPSGFNLIFDVRKTSLDLYLFYLEVTKRGGYHQVDEEKKWGEVVSALKLEGNNATLCDQLEKLYKELLYKFETLYFYRSPATQTATGSNTGPVERNQNSTTSLSQLMDEQDYQKAAKISEDYSSQITGVGYQEFQVVQQAPAKNKEKKKRRGAPIGQSGYNIFLKQECARLKAYHQDVGGRKIIEMAIDAWNKLSDKEKQPYEEASVKIKEEIKEAPTNNKEKKKHRGVPRGQKSAYQIFLKHECARLKADHQFKGDRKITAIDAWKMMSPMEKLPYEEESMKIKEEIKEAMIQKSTEDLERDEERPIVCGDYYSVTSQPLSNYSFGNNAVVDLDFKLTEKTSEDPFFPGDLNDYRSLDLPTGEPE*