AlfalfaGEDB | MsG0680031788.01

M. sativa cultivar ZhongmuNo.1 / MsG0680031788.01

KEGG: K03549
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031788.01.T01	KAF7801922.1	87.805	41	5	0	1	41	17	57	1.32E-16	80.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031788.01.T01	A0A834VYD9	87.805	41	5	0	1	41	17	57	6.28e-17	80.9

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280006319.01	MsG0680031788.01	0.809025	2.320285e-50	1.347298e-47
MsG0380012931.01	MsG0680031788.01	0.812995	3.202625e-51	2.066421e-48
MsG0380014646.01	MsG0680031788.01	0.864184	1.459677e-64	4.542257e-61
MsG0480018089.01	MsG0680031788.01	0.816209	6.222376e-52	4.381742e-49
MsG0480021420.01	MsG0680031788.01	0.806557	7.762002e-50	4.225928e-47
MsG0480022300.01	MsG0680031788.01	0.834010	3.837627e-56	4.495983e-53
MsG0480023151.01	MsG0680031788.01	0.809711	1.653039e-50	9.776459e-48
MsG0480023395.01	MsG0680031788.01	0.807930	3.972958e-50	2.241341e-47
MsG0580024538.01	MsG0680031788.01	0.830488	2.854487e-55	3.011644e-52
MsG0580024567.01	MsG0680031788.01	0.806769	7.002329e-50	3.833025e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031788.01.T01	MTR_6g033165	100.000	36	0	0	6	41	735	770	4.60e-18	79.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031788.01.T01	AT3G02050	69.048	42	12	1	1	41	699	740	2.65e-11	60.5

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Find 29 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTAAGAAGGCGTCGAGTA+AGG	0.442966	6:-26781783	None:intergenic
AATGTCGATTTGTAACTACT+AGG	0.618260	6:+26781472	None:intergenic
ACTGCCTAACAACCCTGGAT+TGG	0.494344	6:-26781755	MsG0680031788.01.T01:CDS
ACTTCGATAAGACTGATGTG+AGG	0.732857	6:+26781549	None:intergenic
AGATTTAATCCAAGCCAAGG+AGG	0.711722	6:-26781707	MsG0680031788.01.T01:CDS
AGGATGTAAAATTTACAAAA+TGG	0.320235	6:+26781386	None:intergenic
ATAATGGGACACTCGTATGA+AGG	0.577371	6:-26781670	MsG0680031788.01.T01:CDS
ATACGCAACTCCAGCCTCCT+TGG	0.478895	6:+26781693	None:intergenic
CAACTCCAGCCTCCTTGGCT+TGG	0.448016	6:+26781698	None:intergenic
CAATGACCATCATTCAAGTA+TGG	0.415193	6:+26781357	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGCTTGTGATCGATAT+GGG	-	Chr6:26781517-26781536	MsG0680031788.01.T01:CDS	30.0%
CTAGGAAAATCTAACCTAAA+TGG	+	Chr6:26781654-26781673	None:intergenic	30.0%
TCATTCAAGTATGGTGAAAA+AGG	+	Chr6:26781778-26781797	None:intergenic	30.0%
TTTAGATTTAATCCAAGCCA+AGG	-	Chr6:26781431-26781450	MsG0680031788.01.T01:CDS	30.0%
GAAAAAGCTTGTGATCGATA+TGG	-	Chr6:26781516-26781535	MsG0680031788.01.T01:CDS	35.0%
ACTTCGATAAGACTGATGTG+AGG	+	Chr6:26781595-26781614	None:intergenic	40.0%
AGATTTAATCCAAGCCAAGG+AGG	-	Chr6:26781434-26781453	MsG0680031788.01.T01:CDS	40.0%
GCTGGAGTTGCGTATATAAT+GGG	-	Chr6:26781456-26781475	MsG0680031788.01.T01:CDS	40.0%
GTTTCTGCGAAAGAATTGCA+GGG	-	Chr6:26781546-26781565	MsG0680031788.01.T01:CDS	40.0%
TAACAGCATGATCCAATCCA+GGG	+	Chr6:26781401-26781420	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	26781366	26781797	26781366	ID=MsG0680031788.01;Name=MsG0680031788.01
Chr6	mRNA	26781366	26781797	26781366	ID=MsG0680031788.01.T01;Parent=MsG0680031788.01;Name=MsG0680031788.01.T01;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|143
Chr6	exon	26781366	26781797	26781366	ID=MsG0680031788.01.T01:exon:8469;Parent=MsG0680031788.01.T01
Chr6	CDS	26781366	26781797	26781366	ID=MsG0680031788.01.T01:cds;Parent=MsG0680031788.01.T01

Gene Sequence

>MsG0680031788.01.T01

AGGCGTCGAGTAAGGTTTCAACTGCCTAACAACCCTGGATTGGATCATGCTGTTAAAGAAGAGCTTTTAGATTTAATCCAAGCCAAGGAGGCTGGAGTTGCGTATATAATGGGACACTCGTATGAAGGCAAGGAAATCATCGTCGTTCTTGAAAAAGCTTGTGATCGATATGGGATACTCGTTTCTGCGAAAGAATTGCAGGGGTCCAGCTGTGGCTCTCAACATTCCTCACATCAGTCTTATCGAAGTTGGAATGATATATTATGTCTAGATTGTGGAATTTTACCATTTAGGTTAGATTTTCCTAGTAGTTACAAATCGACATTAGAAACAAGTTTTCAAGATGGAGCCTCATTCTTGATTTTATCATTGCTTCTGCTGGTATACTTCCATTTTGTAAATTTTACATCCTTTTTCACCATACTTGAATGA

Protein sequence

>MsG0680031788.01.T01

RRRVRFQLPNNPGLDHAVKEELLDLIQAKEAGVAYIMGHSYEGKEIIVVLEKACDRYGILVSAKELQGSSCGSQHSSHQSYRSWNDILCLDCGILPFRLDFPSSYKSTLETSFQDGASFLILSLLLLVYFHFVNFTSFFTILE*