Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031788.01.T01 | KAF7801922.1 | 87.805 | 41 | 5 | 0 | 1 | 41 | 17 | 57 | 1.32E-16 | 80.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031788.01.T01 | A0A834VYD9 | 87.805 | 41 | 5 | 0 | 1 | 41 | 17 | 57 | 6.28e-17 | 80.9 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006319.01 | MsG0680031788.01 | 0.809025 | 2.320285e-50 | 1.347298e-47 |
| MsG0380012931.01 | MsG0680031788.01 | 0.812995 | 3.202625e-51 | 2.066421e-48 |
| MsG0380014646.01 | MsG0680031788.01 | 0.864184 | 1.459677e-64 | 4.542257e-61 |
| MsG0680031788.01 | MsG0780041002.01 | 0.827145 | 1.839350e-54 | 1.760500e-51 |
| MsG0680031788.01 | MsG0780041218.01 | 0.828570 | 8.352576e-55 | 8.334230e-52 |
| MsG0680031788.01 | MsG0880046574.01 | 0.816365 | 5.742327e-52 | 4.060849e-49 |
| MsG0680031788.01 | MsG0880046735.01 | 0.816748 | 4.713270e-52 | 3.368131e-49 |
| MsG0680031788.01 | MsG0880046856.01 | 0.830721 | 2.503161e-55 | 2.659441e-52 |
| MsG0680031788.01 | MsG0880047660.01 | 0.817674 | 2.916735e-52 | 2.137953e-49 |
| MsG0480021420.01 | MsG0680031788.01 | 0.806557 | 7.762002e-50 | 4.225928e-47 |
| MsG0480022300.01 | MsG0680031788.01 | 0.834010 | 3.837627e-56 | 4.495983e-53 |
| MsG0480023151.01 | MsG0680031788.01 | 0.809711 | 1.653039e-50 | 9.776459e-48 |
| MsG0480023395.01 | MsG0680031788.01 | 0.807930 | 3.972958e-50 | 2.241341e-47 |
| MsG0580024538.01 | MsG0680031788.01 | 0.830488 | 2.854487e-55 | 3.011644e-52 |
| MsG0580024567.01 | MsG0680031788.01 | 0.806769 | 7.002329e-50 | 3.833025e-47 |
| MsG0580024763.01 | MsG0680031788.01 | 0.814267 | 1.680911e-51 | 1.122674e-48 |
| MsG0580024766.01 | MsG0680031788.01 | 0.840482 | 8.466682e-58 | 1.207634e-54 |
| MsG0480018089.01 | MsG0680031788.01 | 0.816209 | 6.222376e-52 | 4.381742e-49 |
| MsG0580027098.01 | MsG0680031788.01 | 0.800801 | 1.215128e-48 | 5.710217e-46 |
| MsG0580029706.01 | MsG0680031788.01 | 0.807794 | 4.246658e-50 | 2.387355e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031788.01.T01 | MTR_6g033165 | 100.000 | 36 | 0 | 0 | 6 | 41 | 735 | 770 | 4.60e-18 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031788.01.T01 | AT3G02050 | 69.048 | 42 | 12 | 1 | 1 | 41 | 699 | 740 | 2.65e-11 | 60.5 |
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGGAAAATCTAACCTAAA+TGG | 0.297810 | 6:+26781490 | None:intergenic |
| TCATTCAAGTATGGTGAAAA+AGG | 0.303717 | 6:+26781366 | None:intergenic |
| AGGATGTAAAATTTACAAAA+TGG | 0.320235 | 6:+26781386 | None:intergenic |
| GGCTGGAGTTGCGTATATAA+TGG | 0.328115 | 6:-26781686 | MsG0680031788.01.T01:CDS |
| CGTTTCTGCGAAAGAATTGC+AGG | 0.376232 | 6:-26781596 | MsG0680031788.01.T01:CDS |
| TGATCCAATCCAGGGTTGTT+AGG | 0.412649 | 6:+26781751 | None:intergenic |
| CAATGACCATCATTCAAGTA+TGG | 0.415193 | 6:+26781357 | None:intergenic |
| CACATCAGTCTTATCGAAGT+TGG | 0.424667 | 6:-26781546 | MsG0680031788.01.T01:CDS |
| AAGTAAGAAGGCGTCGAGTA+AGG | 0.442966 | 6:-26781783 | None:intergenic |
| CAACTCCAGCCTCCTTGGCT+TGG | 0.448016 | 6:+26781698 | None:intergenic |
| GCTGGAGTTGCGTATATAAT+GGG | 0.459552 | 6:-26781685 | MsG0680031788.01.T01:CDS |
| ATACGCAACTCCAGCCTCCT+TGG | 0.478895 | 6:+26781693 | None:intergenic |
| TTAACAGCATGATCCAATCC+AGG | 0.482648 | 6:+26781742 | None:intergenic |
| TTAATCCAAGCCAAGGAGGC+TGG | 0.483772 | 6:-26781703 | MsG0680031788.01.T01:CDS |
| ACTGCCTAACAACCCTGGAT+TGG | 0.494344 | 6:-26781755 | MsG0680031788.01.T01:CDS |
| GATATATTATGTCTAGATTG+TGG | 0.513129 | 6:-26781520 | MsG0680031788.01.T01:CDS |
| TTTAGATTTAATCCAAGCCA+AGG | 0.535244 | 6:-26781710 | MsG0680031788.01.T01:CDS |
| GGAATGTTGAGAGCCACAGC+TGG | 0.564106 | 6:+26781570 | None:intergenic |
| TTTCAACTGCCTAACAACCC+TGG | 0.571708 | 6:-26781760 | MsG0680031788.01.T01:CDS |
| CAATGATAAAATCAAGAATG+AGG | 0.575181 | 6:+26781426 | None:intergenic |
| ATAATGGGACACTCGTATGA+AGG | 0.577371 | 6:-26781670 | MsG0680031788.01.T01:CDS |
| GGGACACTCGTATGAAGGCA+AGG | 0.601079 | 6:-26781665 | MsG0680031788.01.T01:CDS |
| GTTTCTGCGAAAGAATTGCA+GGG | 0.607197 | 6:-26781595 | MsG0680031788.01.T01:CDS |
| TAACAGCATGATCCAATCCA+GGG | 0.608772 | 6:+26781743 | None:intergenic |
| GAATTGCAGGGGTCCAGCTG+TGG | 0.613283 | 6:-26781583 | MsG0680031788.01.T01:CDS |
| AATGTCGATTTGTAACTACT+AGG | 0.618260 | 6:+26781472 | None:intergenic |
| TTTCTGCGAAAGAATTGCAG+GGG | 0.665372 | 6:-26781594 | MsG0680031788.01.T01:CDS |
| AGATTTAATCCAAGCCAAGG+AGG | 0.711722 | 6:-26781707 | MsG0680031788.01.T01:CDS |
| ACTTCGATAAGACTGATGTG+AGG | 0.732857 | 6:+26781549 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGGATGTAAAATTTACAAAA+TGG | + | Chr6:26781758-26781777 | None:intergenic | 20.0% |
| ! | CAATGATAAAATCAAGAATG+AGG | + | Chr6:26781718-26781737 | None:intergenic | 25.0% |
| ! | GATATATTATGTCTAGATTG+TGG | - | Chr6:26781621-26781640 | MsG0680031788.01.T01:CDS | 25.0% |
| !! | ATTGTGGAATTTTACCATTT+AGG | - | Chr6:26781637-26781656 | MsG0680031788.01.T01:CDS | 25.0% |
| !!! | TTAGAAACAAGTTTTCAAGA+TGG | - | Chr6:26781690-26781709 | MsG0680031788.01.T01:CDS | 25.0% |
| AAAAAGCTTGTGATCGATAT+GGG | - | Chr6:26781517-26781536 | MsG0680031788.01.T01:CDS | 30.0% | |
| CTAGGAAAATCTAACCTAAA+TGG | + | Chr6:26781654-26781673 | None:intergenic | 30.0% | |
| TCATTCAAGTATGGTGAAAA+AGG | + | Chr6:26781778-26781797 | None:intergenic | 30.0% | |
| TTTAGATTTAATCCAAGCCA+AGG | - | Chr6:26781431-26781450 | MsG0680031788.01.T01:CDS | 30.0% | |
| ! | TTTTCACCATACTTGAATGA+AGG | - | Chr6:26781778-26781797 | MsG0680031788.01.T01:CDS | 30.0% |
| !! | AATGTCGATTTGTAACTACT+AGG | + | Chr6:26781672-26781691 | None:intergenic | 30.0% |
| GAAAAAGCTTGTGATCGATA+TGG | - | Chr6:26781516-26781535 | MsG0680031788.01.T01:CDS | 35.0% | |
| ! | GATTTTATCATTGCTTCTGC+TGG | - | Chr6:26781725-26781744 | MsG0680031788.01.T01:CDS | 35.0% |
| ACTTCGATAAGACTGATGTG+AGG | + | Chr6:26781595-26781614 | None:intergenic | 40.0% | |
| AGATTTAATCCAAGCCAAGG+AGG | - | Chr6:26781434-26781453 | MsG0680031788.01.T01:CDS | 40.0% | |
| GCTGGAGTTGCGTATATAAT+GGG | - | Chr6:26781456-26781475 | MsG0680031788.01.T01:CDS | 40.0% | |
| GTTTCTGCGAAAGAATTGCA+GGG | - | Chr6:26781546-26781565 | MsG0680031788.01.T01:CDS | 40.0% | |
| TAACAGCATGATCCAATCCA+GGG | + | Chr6:26781401-26781420 | None:intergenic | 40.0% | |
| TTAACAGCATGATCCAATCC+AGG | + | Chr6:26781402-26781421 | None:intergenic | 40.0% | |
| TTTCTGCGAAAGAATTGCAG+GGG | - | Chr6:26781547-26781566 | MsG0680031788.01.T01:CDS | 40.0% | |
| ! | ATAATGGGACACTCGTATGA+AGG | - | Chr6:26781471-26781490 | MsG0680031788.01.T01:CDS | 40.0% |
| ! | CACATCAGTCTTATCGAAGT+TGG | - | Chr6:26781595-26781614 | MsG0680031788.01.T01:CDS | 40.0% |
| CGTTTCTGCGAAAGAATTGC+AGG | - | Chr6:26781545-26781564 | MsG0680031788.01.T01:CDS | 45.0% | |
| GGCTGGAGTTGCGTATATAA+TGG | - | Chr6:26781455-26781474 | MsG0680031788.01.T01:CDS | 45.0% | |
| TGATCCAATCCAGGGTTGTT+AGG | + | Chr6:26781393-26781412 | None:intergenic | 45.0% | |
| TTTCAACTGCCTAACAACCC+TGG | - | Chr6:26781381-26781400 | MsG0680031788.01.T01:CDS | 45.0% | |
| ACTGCCTAACAACCCTGGAT+TGG | - | Chr6:26781386-26781405 | MsG0680031788.01.T01:CDS | 50.0% | |
| TTAATCCAAGCCAAGGAGGC+TGG | - | Chr6:26781438-26781457 | MsG0680031788.01.T01:CDS | 50.0% | |
| ATACGCAACTCCAGCCTCCT+TGG | + | Chr6:26781451-26781470 | None:intergenic | 55.0% | |
| GGAATGTTGAGAGCCACAGC+TGG | + | Chr6:26781574-26781593 | None:intergenic | 55.0% | |
| GGGACACTCGTATGAAGGCA+AGG | - | Chr6:26781476-26781495 | MsG0680031788.01.T01:CDS | 55.0% | |
| CAACTCCAGCCTCCTTGGCT+TGG | + | Chr6:26781446-26781465 | None:intergenic | 60.0% | |
| GAATTGCAGGGGTCCAGCTG+TGG | - | Chr6:26781558-26781577 | MsG0680031788.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 26781366 | 26781797 | 26781366 | ID=MsG0680031788.01;Name=MsG0680031788.01 |
| Chr6 | mRNA | 26781366 | 26781797 | 26781366 | ID=MsG0680031788.01.T01;Parent=MsG0680031788.01;Name=MsG0680031788.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|143 |
| Chr6 | exon | 26781366 | 26781797 | 26781366 | ID=MsG0680031788.01.T01:exon:8469;Parent=MsG0680031788.01.T01 |
| Chr6 | CDS | 26781366 | 26781797 | 26781366 | ID=MsG0680031788.01.T01:cds;Parent=MsG0680031788.01.T01 |
| Gene Sequence |
| Protein sequence |
