AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022300.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCCAGCCTTCTGATTCT+TGG 0.181095 4:+70848341 None:intergenic
CATTCAGTTGGATTCATGAT+AGG 0.310680 4:-70848280 MsG0480022300.01.T01:CDS
GTACTACTCTAAGCATATTA+AGG 0.323956 4:-70848254 MsG0480022300.01.T01:CDS
AATCCAACTGAATGCAAGTT+TGG 0.355409 4:+70848289 None:intergenic
AATCTTCCAGCAACTCTACT+TGG 0.387112 4:-70848222 MsG0480022300.01.T01:CDS
ATTGCTCCAAGTAGAGTTGC+TGG 0.409276 4:+70848216 None:intergenic
CATCCAAACTTGCATTCAGT+TGG 0.428488 4:-70848292 MsG0480022300.01.T01:CDS
TCCAGCCTTCTGATTCTTGG+TGG 0.465016 4:+70848344 None:intergenic
AAGGCACAGGAGTATGTTTG+TGG 0.492271 4:-70848371 None:intergenic
ATTCAGTTGGATTCATGATA+GGG 0.509293 4:-70848279 MsG0480022300.01.T01:CDS
GCCACCAAGAATCAGAAGGC+TGG 0.541195 4:-70848345 MsG0480022300.01.T01:CDS
AGGCACAGGAGTATGTTTGT+GGG 0.574102 4:-70848370 None:intergenic
GAGAGCTTCAGCAAGAGCAA+AGG 0.595934 4:-70848318 MsG0480022300.01.T01:CDS
GGTTGCCACCAAGAATCAGA+AGG 0.645168 4:-70848349 MsG0480022300.01.T01:CDS
ACCAAGAATCAGAAGGCTGG+AGG 0.675099 4:-70848342 MsG0480022300.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAGTTTGTCATTTTTGTT+TGG - Chr4:70848355-70848374 MsG0480022300.01.T01:CDS 25.0%
ATTCAGTTGGATTCATGATA+GGG - Chr4:70848253-70848272 MsG0480022300.01.T01:CDS 30.0%
GTACTACTCTAAGCATATTA+AGG - Chr4:70848278-70848297 MsG0480022300.01.T01:CDS 30.0%
AATCCAACTGAATGCAAGTT+TGG + Chr4:70848246-70848265 None:intergenic 35.0%
CATTCAGTTGGATTCATGAT+AGG - Chr4:70848252-70848271 MsG0480022300.01.T01:CDS 35.0%
AATCTTCCAGCAACTCTACT+TGG - Chr4:70848310-70848329 MsG0480022300.01.T01:CDS 40.0%
CATCCAAACTTGCATTCAGT+TGG - Chr4:70848240-70848259 MsG0480022300.01.T01:CDS 40.0%
ATTGCTCCAAGTAGAGTTGC+TGG + Chr4:70848319-70848338 None:intergenic 45.0%
ACCAAGAATCAGAAGGCTGG+AGG - Chr4:70848190-70848209 MsG0480022300.01.T01:CDS 50.0%
GAGAGCTTCAGCAAGAGCAA+AGG - Chr4:70848214-70848233 MsG0480022300.01.T01:CDS 50.0%
GGTTGCCACCAAGAATCAGA+AGG - Chr4:70848183-70848202 MsG0480022300.01.T01:CDS 50.0%
TCCAGCCTTCTGATTCTTGG+TGG + Chr4:70848191-70848210 None:intergenic 50.0%
TCCTCCAGCCTTCTGATTCT+TGG + Chr4:70848194-70848213 None:intergenic 50.0%
GCCACCAAGAATCAGAAGGC+TGG - Chr4:70848187-70848206 MsG0480022300.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70848174 70848380 70848174 ID=MsG0480022300.01;Name=MsG0480022300.01
Chr4 mRNA 70848174 70848380 70848174 ID=MsG0480022300.01.T01;Parent=MsG0480022300.01;Name=MsG0480022300.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|68
Chr4 exon 70848174 70848380 70848174 ID=MsG0480022300.01.T01:exon:5664;Parent=MsG0480022300.01.T01
Chr4 CDS 70848174 70848380 70848174 ID=MsG0480022300.01.T01:cds;Parent=MsG0480022300.01.T01
Gene Sequence

>MsG0480022300.01.T01

ATGTTTGTGGGTTGCCACCAAGAATCAGAAGGCTGGAGGAGAGAGCTTCAGCAAGAGCAAAGGAATCATCCAAACTTGCATTCAGTTGGATTCATGATAGGGAAGTACTACTCTAAGCATATTAAGGATCATATTAAATCTTCCAGCAACTCTACTTGGAGCAATGTTGAACAGTTGAAAATTCAGTTTGTCATTTTTGTTTGGTGA

Protein sequence

>MsG0480022300.01.T01

MFVGCHQESEGWRRELQQEQRNHPNLHSVGFMIGKYYSKHIKDHIKSSSNSTWSNVEQLKIQFVIFVW*