AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033646.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033646.01.T01 MTR_0019s0160 96.970 132 4 0 56 187 13 144 1.63e-88 256
MsG0680033646.01.T01 MTR_7g092450 88.596 114 12 1 75 187 32 145 2.34e-67 202
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033646.01.T01 AT5G51940 80.303 132 25 1 56 187 14 144 1.16e-65 198
MsG0680033646.01.T01 AT2G04630 80.303 132 25 1 56 187 14 144 6.44e-65 196

Find 61 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATGAGCGTGCACGAATTT+TGG 0.183349 6:+70197423 MsG0680033646.01.T01:CDS
TTTCTCTCGAAGCTCTTTCA+TGG 0.342410 6:-70198623 None:intergenic
TCCGAGATGCAGCTCAATTC+CGG 0.345334 6:-70195493 None:intergenic
ACGCTCGGCGTTGACGGTTT+CGG 0.357371 6:+70195466 MsG0680033646.01.T01:CDS
GCTCTTTGATGCGTGGATCT+TGG 0.361114 6:+70195563 MsG0680033646.01.T01:CDS
GACTCATGGAAGAGGCAAAT+TGG 0.371145 6:+70198845 MsG0680033646.01.T01:CDS
GGTGAAACGCTCGGCGTTGA+CGG 0.398585 6:+70195460 MsG0680033646.01.T01:CDS
GCCTATTGAAACCGAGGAAA+AGG 0.427074 6:+70197348 MsG0680033646.01.T01:CDS
GAATTCCTTACTTCAATCTC+AGG 0.430246 6:-70197189 None:intergenic
AATTGTGCAGCTATGAAGAT+TGG 0.435283 6:+70198798 MsG0680033646.01.T01:intron
ATTGTGCAGCTATGAAGATT+GGG 0.436731 6:+70198799 MsG0680033646.01.T01:intron
GTCTTTCGAGGCCTTTCTGT+TGG 0.438965 6:-70197382 None:intergenic
AATTCGTGCACGCTCATACT+TGG 0.443078 6:-70197419 None:intergenic
GTACCCGTGCTCTTCAAATC+AGG 0.451117 6:+70197446 MsG0680033646.01.T01:CDS
GCTCGAGAGCTTCATGAAGC+AGG 0.451772 6:+70195438 None:intergenic
GGCAAATTGGTGGTGATTGA+TGG 0.457288 6:+70198858 MsG0680033646.01.T01:CDS
CATAACCTCTTATTATGCCT+TGG 0.463278 6:+70199009 MsG0680033646.01.T01:three_prime_UTR
ATATATTTCGATGTCTTTCG+AGG 0.473400 6:-70197394 None:intergenic
ACTTCAATCTCAGGATCTAC+TGG 0.475450 6:-70197180 None:intergenic
CAATTGCTGCTGCGTTGCGT+TGG 0.482773 6:+70195527 MsG0680033646.01.T01:CDS
CGTTGACGGTTTCGGCCTGC+CGG 0.483937 6:+70195474 MsG0680033646.01.T01:CDS
CACAGTAATTACCTTCCATC+AGG 0.487928 6:-70198676 None:intergenic
AAGTACCAAGGCATAATAAG+AGG 0.489624 6:-70199014 None:intergenic
CAAGTAACGACGGATGGTAA+AGG 0.490956 6:-70198653 None:intergenic
CCTTCCAACTCAACCATGAC+AGG 0.504355 6:-70197581 None:intergenic
GGGAGTTGATGAATTGATTG+TGG 0.509393 6:+70198820 MsG0680033646.01.T01:CDS
AAGTAACGACGGATGGTAAA+GGG 0.509534 6:-70198652 None:intergenic
GCCGGAATTGAGCTGCATCT+CGG 0.532928 6:+70195492 MsG0680033646.01.T01:CDS
AATTGAGCTGCATCTCGGAT+TGG 0.532983 6:+70195497 MsG0680033646.01.T01:CDS
ATTACCTGATTTGAAGAGCA+CGG 0.540995 6:-70197450 None:intergenic
CTGTCATGGTTGAGTTGGAA+GGG 0.541235 6:+70197582 MsG0680033646.01.T01:CDS
ATCCGTCGTTACTTGCCTGA+TGG 0.543002 6:+70198661 MsG0680033646.01.T01:CDS
TGCACCTGTCATGGTTGAGT+TGG 0.544974 6:+70197577 MsG0680033646.01.T01:CDS
TTACCTGATTTGAAGAGCAC+GGG 0.554617 6:-70197449 None:intergenic
GCGTGGATCTTGGTCTGCCA+TGG 0.560582 6:+70195573 MsG0680033646.01.T01:CDS
ATGAAGCAGGGTGAAACGCT+CGG 0.561853 6:+70195451 MsG0680033646.01.T01:CDS
AGATGCAGCTCAATTCCGGC+AGG 0.564743 6:-70195489 None:intergenic
AATTGATTGTGGAAGACTCA+TGG 0.564886 6:+70198831 MsG0680033646.01.T01:CDS
GACAACAATGATGAGATGGC+TGG 0.565670 6:+70197322 MsG0680033646.01.T01:CDS
AAAGTTTCTCGTACCTCAAG+CGG 0.565990 6:-70197614 None:intergenic
AACAAGAAGAGCCAACAGAA+AGG 0.566880 6:+70197371 MsG0680033646.01.T01:CDS
TGGTGCTTTCATTGACGCCA+TGG 0.577104 6:-70195590 None:intergenic
CTCGAGAGCTTCATGAAGCA+GGG 0.579846 6:+70195439 None:intergenic
GTCGTTACTTGCCTGATGGA+AGG 0.583890 6:+70198665 MsG0680033646.01.T01:CDS
TTGTGGAAGACTCATGGAAG+AGG 0.587346 6:+70198837 MsG0680033646.01.T01:CDS
TTGTAGTGTGGTACTAATGT+TGG 0.589851 6:+70198940 MsG0680033646.01.T01:three_prime_UTR
CCTGTCATGGTTGAGTTGGA+AGG 0.591673 6:+70197581 MsG0680033646.01.T01:CDS
AAGTTTCTCGTACCTCAAGC+GGG 0.593900 6:-70197613 None:intergenic
ATCAGGCAAGTAACGACGGA+TGG 0.598660 6:-70198659 None:intergenic
AGGGGAGACTGACCCGCTTG+AGG 0.598671 6:+70197601 MsG0680033646.01.T01:CDS
AAGTATGAATGCACCTGTCA+TGG 0.600527 6:+70197568 MsG0680033646.01.T01:intron
TTGATAGGAAGGTGCAGAGG+TGG 0.602482 6:+70197285 MsG0680033646.01.T01:intron
TGTCATGGTTGAGTTGGAAG+GGG 0.604272 6:+70197583 MsG0680033646.01.T01:CDS
TCATGGAAGAGGCAAATTGG+TGG 0.616416 6:+70198848 MsG0680033646.01.T01:CDS
CAACGACAACAATGATGAGA+TGG 0.626792 6:+70197318 MsG0680033646.01.T01:CDS
CATAAACTTGACTTGTAGTG+TGG 0.639236 6:+70198928 MsG0680033646.01.T01:three_prime_UTR
TGGAGAGCCTATTGAAACCG+AGG 0.643087 6:+70197342 MsG0680033646.01.T01:CDS
TTCCATCAGGCAAGTAACGA+CGG 0.645675 6:-70198663 None:intergenic
TTGTGCAGCTATGAAGATTG+GGG 0.646097 6:+70198800 MsG0680033646.01.T01:intron
TGGTTGATAGGAAGGTGCAG+AGG 0.655672 6:+70197282 MsG0680033646.01.T01:intron
CAACGCAGCAGCAATTGACA+AGG 0.675175 6:-70195521 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAATTAATAATCAATT+TGG - Chr6:70198489-70198508 None:intergenic 10.0%
!! ATTATTGTAATATGAAAATA+TGG - Chr6:70198052-70198071 None:intergenic 10.0%
!! GAATTAATTATTATCAATAA+AGG - Chr6:70196162-70196181 None:intergenic 10.0%
!! TAATTTAAAAAATTCATCAA+CGG - Chr6:70197261-70197280 None:intergenic 10.0%
!! TCAAAAATGAAAATTAAATT+GGG - Chr6:70196135-70196154 None:intergenic 10.0%
!! TTCAAAAATGAAAATTAAAT+TGG - Chr6:70196136-70196155 None:intergenic 10.0%
!!! TGATGAATTTTTTAAATTAT+TGG + Chr6:70197262-70197281 MsG0680033646.01.T01:intron 10.0%
!! ACAAACATATATGAATATTA+AGG + Chr6:70197229-70197248 MsG0680033646.01.T01:intron 15.0%
!! CAAAAATGAAAATTAAATTG+GGG - Chr6:70196134-70196153 None:intergenic 15.0%
!! TTTACAATCTTATTATTCAA+AGG - Chr6:70198431-70198450 None:intergenic 15.0%
!!! AATAATAACAGCTTTTAATA+TGG + Chr6:70198066-70198085 MsG0680033646.01.T01:intron 15.0%
!!! ATAATAACAGCTTTTAATAT+GGG + Chr6:70198067-70198086 MsG0680033646.01.T01:intron 15.0%
!!! TTTTATTTTGAAATATGGAT+CGG - Chr6:70197969-70197988 None:intergenic 15.0%
!!! TTTTTAAATTATTGGTTGAT+AGG + Chr6:70197270-70197289 MsG0680033646.01.T01:intron 15.0%
!! AAAAGATCATACAGTTTAAA+AGG + Chr6:70197040-70197059 MsG0680033646.01.T01:intron 20.0%
!! AACAAAAGAAATTAGATACT+AGG - Chr6:70197738-70197757 None:intergenic 20.0%
!! AACAAACATAAAAATGAACA+TGG - Chr6:70196236-70196255 None:intergenic 20.0%
!! AATATGAAAATATGGAAAGA+AGG - Chr6:70198044-70198063 None:intergenic 20.0%
!! ATAAGCATTTATCATGATAA+AGG + Chr6:70196675-70196694 MsG0680033646.01.T01:intron 20.0%
!! ATATGAAAATATGGAAAGAA+GGG - Chr6:70198043-70198062 None:intergenic 20.0%
!! ATCATGTTTAAACATTTACA+AGG - Chr6:70197528-70197547 None:intergenic 20.0%
!! GCTTAAAAACATGAATATAT+AGG - Chr6:70196300-70196319 None:intergenic 20.0%
!! TATAGGAAGAAAAAACAAAA+AGG - Chr6:70196283-70196302 None:intergenic 20.0%
!! TCAAAAAATAAAACGATATC+AGG - Chr6:70196184-70196203 None:intergenic 20.0%
!!! AAAAAGGTTACATTTTACTT+TGG - Chr6:70196267-70196286 None:intergenic 20.0%
!!! ATCAATTTGGAATTGATATA+TGG - Chr6:70198476-70198495 None:intergenic 20.0%
!!! TAAACTGTATGATCTTTTTT+AGG - Chr6:70197038-70197057 None:intergenic 20.0%
!!! TAAATATGTTTTGAAGTCAA+TGG - Chr6:70198376-70198395 None:intergenic 20.0%
!!! TCTTTAAAGACATGTATATA+TGG - Chr6:70196751-70196770 None:intergenic 20.0%
!!! TTTATTTTGAAATATGGATC+GGG - Chr6:70197968-70197987 None:intergenic 20.0%
! AAACTTAAACATTCACTTTG+TGG + Chr6:70198259-70198278 MsG0680033646.01.T01:intron 25.0%
! AAGATTGTAAATATCTCCTT+TGG + Chr6:70198440-70198459 MsG0680033646.01.T01:intron 25.0%
! AATTTCAAAACATATCTCGT+CGG + Chr6:70197784-70197803 MsG0680033646.01.T01:intron 25.0%
! ATATTTCATCACTGTTACAT+TGG - Chr6:70195613-70195632 None:intergenic 25.0%
! ATCATACAGTTTAAAAGGAA+TGG + Chr6:70197045-70197064 MsG0680033646.01.T01:intron 25.0%
! ATTACCACACTCAAAAATTA+GGG + Chr6:70196103-70196122 MsG0680033646.01.T01:intron 25.0%
! TACAAATTATACGCAAGAAT+AGG - Chr6:70198148-70198167 None:intergenic 25.0%
! TCATATATGTTTGTGTTAGT+CGG - Chr6:70197223-70197242 None:intergenic 25.0%
! TCCAAAACTAAACAGAAAAA+GGG - Chr6:70198709-70198728 None:intergenic 25.0%
! TCTCTCATAACAAATTTGAA+TGG + Chr6:70197136-70197155 MsG0680033646.01.T01:intron 25.0%
! TTCCAAAACTAAACAGAAAA+AGG - Chr6:70198710-70198729 None:intergenic 25.0%
! TTGAATGTAATAACATGTGT+CGG + Chr6:70196915-70196934 MsG0680033646.01.T01:intron 25.0%
! TTGGAATTGATATATGGTAT+TGG - Chr6:70198470-70198489 None:intergenic 25.0%
! TTTGAACATAATCACATGTT+CGG + Chr6:70196447-70196466 MsG0680033646.01.T01:intron 25.0%
! TTTGTATCTGTTATGAAAGA+CGG - Chr6:70195917-70195936 None:intergenic 25.0%
!! GCTTATTTGAGTTTATCTAA+TGG + Chr6:70196350-70196369 MsG0680033646.01.T01:intron 25.0%
!! GGAAAAATTTGTTCTTTCAA+AGG - Chr6:70197017-70197036 None:intergenic 25.0%
!! GTTTTGTAGTATATTACTAG+TGG + Chr6:70197107-70197126 MsG0680033646.01.T01:intron 25.0%
!! TACATGTCTTTAAAGATTGA+AGG + Chr6:70196755-70196774 MsG0680033646.01.T01:intron 25.0%
!!! TAAATTATTGGTTGATAGGA+AGG + Chr6:70197274-70197293 MsG0680033646.01.T01:intron 25.0%
!!! TCAAATTCATTAGTTTGCAT+GGG + Chr6:70198517-70198536 MsG0680033646.01.T01:intron 25.0%
!!! TTAAGCTTGTTTTGCATTAT+GGG + Chr6:70196314-70196333 MsG0680033646.01.T01:intron 25.0%
!!! TTCAAATTCATTAGTTTGCA+TGG + Chr6:70198516-70198535 MsG0680033646.01.T01:intron 25.0%
!!! TTCTGTTTAGTTTTGGAAAT+GGG + Chr6:70198712-70198731 MsG0680033646.01.T01:intron 25.0%
!!! TTTAAGCTTGTTTTGCATTA+TGG + Chr6:70196313-70196332 MsG0680033646.01.T01:intron 25.0%
!!! TTTCTGTTTAGTTTTGGAAA+TGG + Chr6:70198711-70198730 MsG0680033646.01.T01:intron 25.0%
AGATACTAGGATAACAAGTT+AGG - Chr6:70197725-70197744 None:intergenic 30.0%
ATACAGTTTAAAAGGAATGG+AGG + Chr6:70197048-70197067 MsG0680033646.01.T01:intron 30.0%
ATATATTTCGATGTCTTTCG+AGG - Chr6:70197397-70197416 None:intergenic 30.0%
CAAAACACAATCTCATTAAC+TGG - Chr6:70197093-70197112 None:intergenic 30.0%
CAAAGTGAATGATCAAAGTT+AGG - Chr6:70197862-70197881 None:intergenic 30.0%
CATTACCACACTCAAAAATT+AGG + Chr6:70196102-70196121 MsG0680033646.01.T01:intron 30.0%
CATTATGGGTATGTTTGAAT+TGG + Chr6:70196328-70196347 MsG0680033646.01.T01:intron 30.0%
CTAACTTTGATCATTCACTT+TGG + Chr6:70197860-70197879 MsG0680033646.01.T01:intron 30.0%
TAATGTTGATCGAAAAGAAC+TGG + Chr6:70198330-70198349 MsG0680033646.01.T01:intron 30.0%
TACAAGTGCAGAAATTAGAA+AGG + Chr6:70195650-70195669 MsG0680033646.01.T01:intron 30.0%
TACAGTTTAAAAGGAATGGA+GGG + Chr6:70197049-70197068 MsG0680033646.01.T01:intron 30.0%
TGTGTCCAAAATGTATCAAA+CGG - Chr6:70198566-70198585 None:intergenic 30.0%
TTAAACTGTTTAGCCAAACA+TGG + Chr6:70196718-70196737 MsG0680033646.01.T01:intron 30.0%
TTGTAAATATCTCCTTTGGA+TGG + Chr6:70198444-70198463 MsG0680033646.01.T01:intron 30.0%
! AAACATGATGTCTTGTCTTT+TGG + Chr6:70197539-70197558 MsG0680033646.01.T01:intron 30.0%
! CAGGAACATTTTCATTTACA+GGG - Chr6:70198226-70198245 None:intergenic 30.0%
! CTTAACCGTTTGATACATTT+TGG + Chr6:70198558-70198577 MsG0680033646.01.T01:intron 30.0%
! GTGAAGTTAAGATTTTGTTC+TGG + Chr6:70197493-70197512 MsG0680033646.01.T01:intron 30.0%
! TAACAGCTTTTAATATGGGA+AGG + Chr6:70198071-70198090 MsG0680033646.01.T01:intron 30.0%
! TGTACTAGATCCTCTTTTTA+CGG - Chr6:70195695-70195714 None:intergenic 30.0%
! TTGTCACTGAATGTTGATTA+TGG + Chr6:70198401-70198420 MsG0680033646.01.T01:intron 30.0%
!! TCCCTTTTTCTGTTTAGTTT+TGG + Chr6:70198705-70198724 MsG0680033646.01.T01:intron 30.0%
!!! AAAACCCTAATTTTTGAGTG+TGG - Chr6:70196110-70196129 None:intergenic 30.0%
!!! TTTTTTTTTGTACGTTCTCC+AGG + Chr6:70196579-70196598 MsG0680033646.01.T01:intron 30.0%
AATATCAGAGAGAAGAGAGA+TGG + Chr6:70195745-70195764 MsG0680033646.01.T01:intron 35.0%
AATGAAAATGTTCCTGGCTT+TGG + Chr6:70198230-70198249 MsG0680033646.01.T01:intron 35.0%
AATGGGGGAAGATTTCTTTA+AGG + Chr6:70197831-70197850 MsG0680033646.01.T01:intron 35.0%
AATTGATTGTGGAAGACTCA+TGG + Chr6:70198831-70198850 MsG0680033646.01.T01:CDS 35.0%
AATTGTGCAGCTATGAAGAT+TGG + Chr6:70198798-70198817 MsG0680033646.01.T01:intron 35.0%
ACTGCTAATGTGCTAATGTA+TGG + Chr6:70198001-70198020 MsG0680033646.01.T01:intron 35.0%
AGTGATGAAATATGCAGTAC+AGG + Chr6:70195620-70195639 MsG0680033646.01.T01:intron 35.0%
ATATGGACTTAGACCATGTT+TGG - Chr6:70196734-70196753 None:intergenic 35.0%
ATTGTGCAGCTATGAAGATT+GGG + Chr6:70198799-70198818 MsG0680033646.01.T01:intron 35.0%
CATAACCTCTTATTATGCCT+TGG + Chr6:70199009-70199028 MsG0680033646.01.T01:three_prime_UTR 35.0%
CCTGTAAATGAAAATGTTCC+TGG + Chr6:70198224-70198243 MsG0680033646.01.T01:intron 35.0%
CTGCTAATGTGCTAATGTAT+GGG + Chr6:70198002-70198021 MsG0680033646.01.T01:intron 35.0%
CTTATTCCTCCAAGTTAGTT+GGG + Chr6:70195859-70195878 MsG0680033646.01.T01:intron 35.0%
GAATTCCTTACTTCAATCTC+AGG - Chr6:70197192-70197211 None:intergenic 35.0%
GAATTTGGACACAAAACAAG+CGG + Chr6:70195972-70195991 MsG0680033646.01.T01:intron 35.0%
TAGATCCTGAGATTGAAGTA+AGG + Chr6:70197184-70197203 MsG0680033646.01.T01:intron 35.0%
TCTTATTCCTCCAAGTTAGT+TGG + Chr6:70195858-70195877 MsG0680033646.01.T01:intron 35.0%
TGGTAAGGGGTTTAAGAATT+TGG + Chr6:70195957-70195976 MsG0680033646.01.T01:intron 35.0%
TTGCAAAAACAGAAAACGCA+TGG - Chr6:70198778-70198797 None:intergenic 35.0%
TTGTAGTGTGGTACTAATGT+TGG + Chr6:70198940-70198959 MsG0680033646.01.T01:three_prime_UTR 35.0%
! AAAGTGTCTTCTTCTGGTAA+GGG + Chr6:70195943-70195962 MsG0680033646.01.T01:intron 35.0%
! ATTACCTGATTTGAAGAGCA+CGG - Chr6:70197453-70197472 None:intergenic 35.0%
! CCAGGAACATTTTCATTTAC+AGG - Chr6:70198227-70198246 None:intergenic 35.0%
! GCATTTGAGTTATGTCTGAA+GGG + Chr6:70197680-70197699 MsG0680033646.01.T01:intron 35.0%
! GTACTAGATCCTCTTTTTAC+GGG - Chr6:70195694-70195713 None:intergenic 35.0%
! TGCATTTGAGTTATGTCTGA+AGG + Chr6:70197679-70197698 MsG0680033646.01.T01:intron 35.0%
!! AAGTTTTAATGTCCAAAGCC+AGG - Chr6:70198245-70198264 None:intergenic 35.0%
!! CATAAACTTGACTTGTAGTG+TGG + Chr6:70198928-70198947 MsG0680033646.01.T01:three_prime_UTR 35.0%
!!! ACAGAGGTTCTAAGACTTTT+TGG + Chr6:70196503-70196522 MsG0680033646.01.T01:intron 35.0%
AAAGTTTCTCGTACCTCAAG+CGG - Chr6:70197617-70197636 None:intergenic 40.0%
AACAAGAAGAGCCAACAGAA+AGG + Chr6:70197371-70197390 MsG0680033646.01.T01:CDS 40.0%
AAGTAACGACGGATGGTAAA+GGG - Chr6:70198655-70198674 None:intergenic 40.0%
AAGTATGAATGCACCTGTCA+TGG + Chr6:70197568-70197587 MsG0680033646.01.T01:intron 40.0%
AAGTGTCTTCTTCTGGTAAG+GGG + Chr6:70195944-70195963 MsG0680033646.01.T01:intron 40.0%
ACTTCAATCTCAGGATCTAC+TGG - Chr6:70197183-70197202 None:intergenic 40.0%
AGGCTCAAGGGTTATAAAGT+AGG - Chr6:70195809-70195828 None:intergenic 40.0%
AGTCAAGGACTCCATTAACT+AGG - Chr6:70197896-70197915 None:intergenic 40.0%
AGTGGTATTCGTATGGAATG+GGG + Chr6:70197815-70197834 MsG0680033646.01.T01:intron 40.0%
ATAGACCAGTGGTATTCGTA+TGG + Chr6:70197808-70197827 MsG0680033646.01.T01:intron 40.0%
ATTACCAGTGTAGACATGTC+TGG - Chr6:70196408-70196427 None:intergenic 40.0%
CAACGACAACAATGATGAGA+TGG + Chr6:70197318-70197337 MsG0680033646.01.T01:CDS 40.0%
CACAGTAATTACCTTCCATC+AGG - Chr6:70198679-70198698 None:intergenic 40.0%
CAGTGGTATTCGTATGGAAT+GGG + Chr6:70197814-70197833 MsG0680033646.01.T01:intron 40.0%
CTAACTTGGAGGAATAAGAG+AGG - Chr6:70195857-70195876 None:intergenic 40.0%
GATCTAGTACAATGTTGCAG+AGG + Chr6:70195704-70195723 MsG0680033646.01.T01:intron 40.0%
GCATGTGAGGAATGAGAATT+AGG - Chr6:70195787-70195806 None:intergenic 40.0%
GGTTATAAAGTAGGCATGTG+AGG - Chr6:70195800-70195819 None:intergenic 40.0%
TGATCAACTCCCGTAAAAAG+AGG + Chr6:70195682-70195701 MsG0680033646.01.T01:intron 40.0%
TTGTGCAGCTATGAAGATTG+GGG + Chr6:70198800-70198819 MsG0680033646.01.T01:intron 40.0%
TTTCTCTCGAAGCTCTTTCA+TGG - Chr6:70198626-70198645 None:intergenic 40.0%
! AAGCACAAAGTGTCTTCTTC+TGG + Chr6:70195937-70195956 MsG0680033646.01.T01:intron 40.0%
! ACATTTTCATTTACAGGGCC+AGG - Chr6:70198221-70198240 None:intergenic 40.0%
! ACTACAATGACTTGGACATC+GGG + Chr6:70196036-70196055 MsG0680033646.01.T01:intron 40.0%
! CAAAGTGTCTTCTTCTGGTA+AGG + Chr6:70195942-70195961 MsG0680033646.01.T01:intron 40.0%
! CATTTTCATTTACAGGGCCA+GGG - Chr6:70198220-70198239 None:intergenic 40.0%
! CTCTTCTTGTTCCTTTTCCT+CGG - Chr6:70197362-70197381 None:intergenic 40.0%
! GACTACAATGACTTGGACAT+CGG + Chr6:70196035-70196054 MsG0680033646.01.T01:intron 40.0%
! TATGAGCGTGCACGAATTTT+GGG + Chr6:70197424-70197443 MsG0680033646.01.T01:CDS 40.0%
! TCCTTTTCCTCGGTTTCAAT+AGG - Chr6:70197352-70197371 None:intergenic 40.0%
! TTACCTGATTTGAAGAGCAC+GGG - Chr6:70197452-70197471 None:intergenic 40.0%
!! ATATGGTATTGGCCATCCAA+AGG - Chr6:70198459-70198478 None:intergenic 40.0%
!! CATAATCACATGTTCGGTGT+TGG + Chr6:70196453-70196472 MsG0680033646.01.T01:intron 40.0%
!! GCTATTTCTATAACCTGTCC+TGG - Chr6:70196600-70196619 None:intergenic 40.0%
!! GGGAGTTGATGAATTGATTG+TGG + Chr6:70198820-70198839 MsG0680033646.01.T01:CDS 40.0%
!!! TAGTTTTGGAAATGGGTGAG+TGG + Chr6:70198719-70198738 MsG0680033646.01.T01:intron 40.0%
AACCCTTGAGCCTAGTGTTA+AGG + Chr6:70195816-70195835 MsG0680033646.01.T01:intron 45.0%
AAGTTTCTCGTACCTCAAGC+GGG - Chr6:70197616-70197635 None:intergenic 45.0%
AATTCGTGCACGCTCATACT+TGG - Chr6:70197422-70197441 None:intergenic 45.0%
AATTGAGCTGCATCTCGGAT+TGG + Chr6:70195497-70195516 MsG0680033646.01.T01:CDS 45.0%
ACAGAAAACGCATGGCATGA+AGG - Chr6:70198770-70198789 None:intergenic 45.0%
AGTGTAGACATGTCTGGTGT+CGG - Chr6:70196402-70196421 None:intergenic 45.0%
AGTTCATGTTTCCTTTGCCC+TGG + Chr6:70198200-70198219 MsG0680033646.01.T01:intron 45.0%
CAAGGACTCCATTAACTAGG+AGG - Chr6:70197893-70197912 None:intergenic 45.0%
CAAGTAACGACGGATGGTAA+AGG - Chr6:70198656-70198675 None:intergenic 45.0%
CAAGTCATTGTAGTCGTCGT+CGG - Chr6:70196030-70196049 None:intergenic 45.0%
CACAGCCATAGTTAGAGTCA+AGG - Chr6:70197911-70197930 None:intergenic 45.0%
CCAGTGGTATTCGTATGGAA+TGG + Chr6:70197813-70197832 MsG0680033646.01.T01:intron 45.0%
CCATTCCATACGAATACCAC+TGG - Chr6:70197816-70197835 None:intergenic 45.0%
CTTGACTCTAACTATGGCTG+TGG + Chr6:70197909-70197928 MsG0680033646.01.T01:intron 45.0%
GACAACAATGATGAGATGGC+TGG + Chr6:70197322-70197341 MsG0680033646.01.T01:CDS 45.0%
GACTCATGGAAGAGGCAAAT+TGG + Chr6:70198845-70198864 MsG0680033646.01.T01:CDS 45.0%
GACTCCATTAACTAGGAGGA+GGG - Chr6:70197889-70197908 None:intergenic 45.0%
GCCTATTGAAACCGAGGAAA+AGG + Chr6:70197348-70197367 MsG0680033646.01.T01:CDS 45.0%
GGAGTCCTTGACTCTAACTA+TGG + Chr6:70197903-70197922 MsG0680033646.01.T01:intron 45.0%
GGCAGGGTTGTCTTTAAATC+AGG + Chr6:70198109-70198128 MsG0680033646.01.T01:intron 45.0%
GTAAGGGTTTCTCGTCACAA+TGG + Chr6:70196004-70196023 MsG0680033646.01.T01:intron 45.0%
GTCCTTAACACTAGGCTCAA+GGG - Chr6:70195821-70195840 None:intergenic 45.0%
GTGGTATTCGTATGGAATGG+GGG + Chr6:70197816-70197835 MsG0680033646.01.T01:intron 45.0%
GTTATGAAAGACGGCCTAGA+AGG - Chr6:70195908-70195927 None:intergenic 45.0%
TACTACCAGTGTCAACGTGT+CGG - Chr6:70196870-70196889 None:intergenic 45.0%
TCATGGAAGAGGCAAATTGG+TGG + Chr6:70198848-70198867 MsG0680033646.01.T01:CDS 45.0%
TGTCCTTAACACTAGGCTCA+AGG - Chr6:70195822-70195841 None:intergenic 45.0%
TTCCATCAGGCAAGTAACGA+CGG - Chr6:70198666-70198685 None:intergenic 45.0%
TTGTGGAAGACTCATGGAAG+AGG + Chr6:70198837-70198856 MsG0680033646.01.T01:CDS 45.0%
! CGTTGCGTTGGATGTCTTTT+TGG + Chr6:70195539-70195558 MsG0680033646.01.T01:CDS 45.0%
! GTATGAGCGTGCACGAATTT+TGG + Chr6:70197423-70197442 MsG0680033646.01.T01:CDS 45.0%
! TTTTGTACGTTCTCCAGGAC+AGG + Chr6:70196584-70196603 MsG0680033646.01.T01:intron 45.0%
!! CTGTCATGGTTGAGTTGGAA+GGG + Chr6:70197582-70197601 MsG0680033646.01.T01:CDS 45.0%
!! GCTGATTTTCTTGTGAGGCA+GGG + Chr6:70198093-70198112 MsG0680033646.01.T01:intron 45.0%
!! GGAAGGCTGATTTTCTTGTG+AGG + Chr6:70198088-70198107 MsG0680033646.01.T01:intron 45.0%
!! GGCAAATTGGTGGTGATTGA+TGG + Chr6:70198858-70198877 MsG0680033646.01.T01:CDS 45.0%
!! TGAACTATGAAGCACCGACA+CGG + Chr6:70196830-70196849 MsG0680033646.01.T01:intron 45.0%
!! TGTCATGGTTGAGTTGGAAG+GGG + Chr6:70197583-70197602 MsG0680033646.01.T01:CDS 45.0%
!! TGTCGGTGTCGTGTTGATAT+CGG + Chr6:70196932-70196951 MsG0680033646.01.T01:intron 45.0%
!!! TTTTGGAGCTCTTTGATGCG+TGG + Chr6:70195556-70195575 MsG0680033646.01.T01:CDS 45.0%
!!! TATATTTTTATTTTGAAATA+TGG - Chr6:70197974-70197993 None:intergenic 5.0%
AAGACGGCCTAGAAGGAATG+AGG - Chr6:70195901-70195920 None:intergenic 50.0%
AAGAGGAGGCCCAACTAACT+TGG - Chr6:70195871-70195890 None:intergenic 50.0%
AATGAGGACTCAGCAAGAGG+AGG - Chr6:70195885-70195904 None:intergenic 50.0%
AGGAATGAGGACTCAGCAAG+AGG - Chr6:70195888-70195907 None:intergenic 50.0%
AGTGTTAAGGACACGTGTCC+TGG + Chr6:70195829-70195848 MsG0680033646.01.T01:intron 50.0%
ATCAGGCAAGTAACGACGGA+TGG - Chr6:70198662-70198681 None:intergenic 50.0%
ATCCGTCGTTACTTGCCTGA+TGG + Chr6:70198661-70198680 MsG0680033646.01.T01:CDS 50.0%
ATCTCGTCGGTATAGACCAG+TGG + Chr6:70197797-70197816 MsG0680033646.01.T01:intron 50.0%
ATGAAGCAGGGTGAAACGCT+CGG + Chr6:70195451-70195470 MsG0680033646.01.T01:CDS 50.0%
CAACGCAGCAGCAATTGACA+AGG - Chr6:70195524-70195543 None:intergenic 50.0%
CCTTCCAACTCAACCATGAC+AGG - Chr6:70197584-70197603 None:intergenic 50.0%
CGACGACGACTACAATGACT+TGG + Chr6:70196028-70196047 MsG0680033646.01.T01:intron 50.0%
GACACCAGACATGTCTACAC+TGG + Chr6:70196401-70196420 MsG0680033646.01.T01:intron 50.0%
GCTCTTTGATGCGTGGATCT+TGG + Chr6:70195563-70195582 MsG0680033646.01.T01:CDS 50.0%
GCTGAGTCCTCATTCCTTCT+AGG + Chr6:70195891-70195910 MsG0680033646.01.T01:intron 50.0%
GGACACGTGTCCTTAACACT+AGG - Chr6:70195829-70195848 None:intergenic 50.0%
GGACTCCATTAACTAGGAGG+AGG - Chr6:70197890-70197909 None:intergenic 50.0%
GGAGGAATAAGAGAGGAACC+AGG - Chr6:70195850-70195869 None:intergenic 50.0%
GTACCCGTGCTCTTCAAATC+AGG + Chr6:70197446-70197465 MsG0680033646.01.T01:CDS 50.0%
GTCGTTACTTGCCTGATGGA+AGG + Chr6:70198665-70198684 MsG0680033646.01.T01:CDS 50.0%
GTCTTTCGAGGCCTTTCTGT+TGG - Chr6:70197385-70197404 None:intergenic 50.0%
GTGACCCTCCTCCTAGTTAA+TGG + Chr6:70197882-70197901 MsG0680033646.01.T01:intron 50.0%
TCCGAGATGCAGCTCAATTC+CGG - Chr6:70195496-70195515 None:intergenic 50.0%
TGCACCTGTCATGGTTGAGT+TGG + Chr6:70197577-70197596 MsG0680033646.01.T01:CDS 50.0%
TGGAGAGCCTATTGAAACCG+AGG + Chr6:70197342-70197361 MsG0680033646.01.T01:CDS 50.0%
TGGTGCTTTCATTGACGCCA+TGG - Chr6:70195593-70195612 None:intergenic 50.0%
TGTCAGTGTTGTGTCCGTGT+CGG - Chr6:70196847-70196866 None:intergenic 50.0%
! GCACTGACACATCTGATCGA+AGG - Chr6:70196987-70197006 None:intergenic 50.0%
! TTGATAGGAAGGTGCAGAGG+TGG + Chr6:70197285-70197304 MsG0680033646.01.T01:intron 50.0%
!! ACATGTTCGGTGTTGGACAC+CGG + Chr6:70196460-70196479 MsG0680033646.01.T01:intron 50.0%
!! CCTGTCATGGTTGAGTTGGA+AGG + Chr6:70197581-70197600 MsG0680033646.01.T01:CDS 50.0%
!! GGCTGATTTTCTTGTGAGGC+AGG + Chr6:70198092-70198111 MsG0680033646.01.T01:intron 50.0%
!! TGGTTGATAGGAAGGTGCAG+AGG + Chr6:70197282-70197301 MsG0680033646.01.T01:intron 50.0%
ACAAGCGGAGCTTCAGCGTA+AGG + Chr6:70195987-70196006 MsG0680033646.01.T01:intron 55.0%
ACACCGGACAATCAGAGGTG+TGG + Chr6:70196476-70196495 MsG0680033646.01.T01:intron 55.0%
AGATGCAGCTCAATTCCGGC+AGG - Chr6:70195492-70195511 None:intergenic 55.0%
AGGAGGCCCAACTAACTTGG+AGG - Chr6:70195868-70195887 None:intergenic 55.0%
CAAGCGGAGCTTCAGCGTAA+GGG + Chr6:70195988-70196007 MsG0680033646.01.T01:intron 55.0%
CAATTGCTGCTGCGTTGCGT+TGG + Chr6:70195527-70195546 MsG0680033646.01.T01:CDS 55.0%
CATTTACAGGGCCAGGGCAA+AGG - Chr6:70198214-70198233 None:intergenic 55.0%
GCACCACACCTCTGATTGTC+CGG - Chr6:70196482-70196501 None:intergenic 55.0%
GCCGGAATTGAGCTGCATCT+CGG + Chr6:70195492-70195511 MsG0680033646.01.T01:CDS 55.0%
GTTGGACACCGGACAATCAG+AGG + Chr6:70196471-70196490 MsG0680033646.01.T01:intron 55.0%
TGACACCGACACGTTGACAC+TGG + Chr6:70196862-70196881 MsG0680033646.01.T01:intron 55.0%
! CATCTGATCGAAGGCGTGTC+TGG - Chr6:70196978-70196997 None:intergenic 55.0%
!! GTCTGGTGTCGGTGCTTCAT+AGG - Chr6:70196391-70196410 None:intergenic 55.0%
!! TCAGAGGTGTGGTGCTACAG+AGG + Chr6:70196487-70196506 MsG0680033646.01.T01:intron 55.0%
GCGTGGATCTTGGTCTGCCA+TGG + Chr6:70195573-70195592 MsG0680033646.01.T01:CDS 60.0%
! GGTGAAACGCTCGGCGTTGA+CGG + Chr6:70195460-70195479 MsG0680033646.01.T01:CDS 60.0%
!! ACGCTCGGCGTTGACGGTTT+CGG + Chr6:70195466-70195485 MsG0680033646.01.T01:CDS 60.0%
AGGGGAGACTGACCCGCTTG+AGG + Chr6:70197601-70197620 MsG0680033646.01.T01:CDS 65.0%
CGTTGACGGTTTCGGCCTGC+CGG + Chr6:70195474-70195493 MsG0680033646.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 70195451 70199032 70195451 ID=MsG0680033646.01;Name=MsG0680033646.01
Chr6 mRNA 70195451 70199032 70195451 ID=MsG0680033646.01.T01;Parent=MsG0680033646.01;Name=MsG0680033646.01.T01;_AED=0.53;_eAED=0.53;_QI=0|0.8|0.83|0.83|1|1|6|155|187
Chr6 exon 70198808 70199032 70198808 ID=MsG0680033646.01.T01:exon:1888;Parent=MsG0680033646.01.T01
Chr6 exon 70198619 70198686 70198619 ID=MsG0680033646.01.T01:exon:1887;Parent=MsG0680033646.01.T01
Chr6 exon 70197571 70197622 70197571 ID=MsG0680033646.01.T01:exon:1886;Parent=MsG0680033646.01.T01
Chr6 exon 70197292 70197467 70197292 ID=MsG0680033646.01.T01:exon:1885;Parent=MsG0680033646.01.T01
Chr6 exon 70197167 70197200 70197167 ID=MsG0680033646.01.T01:exon:1884;Parent=MsG0680033646.01.T01
Chr6 exon 70195451 70195614 70195451 ID=MsG0680033646.01.T01:exon:1883;Parent=MsG0680033646.01.T01
Chr6 CDS 70195451 70195614 70195451 ID=MsG0680033646.01.T01:cds;Parent=MsG0680033646.01.T01
Chr6 CDS 70197167 70197200 70197167 ID=MsG0680033646.01.T01:cds;Parent=MsG0680033646.01.T01
Chr6 CDS 70197292 70197467 70197292 ID=MsG0680033646.01.T01:cds;Parent=MsG0680033646.01.T01
Chr6 CDS 70197571 70197622 70197571 ID=MsG0680033646.01.T01:cds;Parent=MsG0680033646.01.T01
Chr6 CDS 70198619 70198686 70198619 ID=MsG0680033646.01.T01:cds;Parent=MsG0680033646.01.T01
Chr6 CDS 70198808 70198877 70198808 ID=MsG0680033646.01.T01:cds;Parent=MsG0680033646.01.T01
Chr6 three_prime_UTR 70198878 70199032 70198878 ID=MsG0680033646.01.T01:three_prime_utr;Parent=MsG0680033646.01.T01
Gene Sequence

>MsG0680033646.01.T01

ATGAAGCAGGGTGAAACGCTCGGCGTTGACGGTTTCGGCCTGCCGGAATTGAGCTGCATCTCGGATTGGACCTTGTCAATTGCTGCTGCGTTGCGTTGGATGTCTTTTTGGAGCTCTTTGATGCGTGGATCTTGGTCTGCCATGGCGTCAATGAAAGCACCAATATATGAAGATGAGCCAGTAGATCCTGAGATTGAAGAAGGTGCAGAGGTGGATGCAGATGACAACGACAACAATGATGAGATGGCTGGAGAGCCTATTGAAACCGAGGAAAAGGAACAAGAAGAGCCAACAGAAAGGCCTCGAAAGACATCGAAATATATGACCAAGTATGAGCGTGCACGAATTTTGGGTACCCGTGCTCTTCAAATCAGTATGAATGCACCTGTCATGGTTGAGTTGGAAGGGGAGACTGACCCGCTTGAGATTGCCATGAAAGAGCTTCGAGAGAAAAAGATACCCTTTACCATCCGTCGTTACTTGCCTGATGGAAGCTATGAAGATTGGGGAGTTGATGAATTGATTGTGGAAGACTCATGGAAGAGGCAAATTGGTGGTGATTGA
Protein sequence

>MsG0680033646.01.T01

MKQGETLGVDGFGLPELSCISDWTLSIAAALRWMSFWSSLMRGSWSAMASMKAPIYEDEPVDPEIEEGAEVDADDNDNNDEMAGEPIETEEKEQEEPTERPRKTSKYMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELREKKIPFTIRRYLPDGSYEDWGVDELIVEDSWKRQIGGD*