AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022790.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022790.01.T01 MTR_4g096810 100.000 129 0 0 11 139 39 167 3.69e-91 262
MsG0480022790.01.T01 MTR_1g475140 39.474 114 66 3 15 127 52 163 1.07e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022790.01.T01 AT5G59180 91.473 129 11 0 11 139 39 167 1.30e-84 245
MsG0480022790.01.T01 AT4G14660 35.156 128 78 4 15 139 47 172 1.94e-16 72.0
MsG0480022790.01.T01 AT4G14660 35.156 128 78 4 15 139 47 172 1.94e-16 72.0
MsG0480022790.01.T01 AT4G14660 35.156 128 78 4 15 139 47 172 1.94e-16 72.0
MsG0480022790.01.T01 AT3G22900 35.398 113 72 1 15 127 46 157 5.01e-16 70.9
MsG0480022790.01.T01 AT3G22900 35.398 113 72 1 15 127 46 157 5.01e-16 70.9

Find 50 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATATTAGCCATTAATTC+TGG 0.172383 4:-76685226 None:intergenic
CTTTGTGTTGATGCAACTTT+TGG 0.190041 4:+76682684 None:intergenic
TGTGCTGTACCCAGAATTAA+TGG 0.225573 4:+76685216 MsG0480022790.01.T01:three_prime_UTR
GAAGTGAGGCTAAAGATAAT+TGG 0.250831 4:+76683711 MsG0480022790.01.T01:CDS
CACCAAGAAATCGTCTTTAA+TGG 0.281864 4:-76684993 None:intergenic
TCTAAGATCTCTCCCTTAAA+TGG 0.305785 4:-76682844 None:intergenic
TCAGTTGATTCCGGATGATA+TGG 0.308719 4:+76683503 MsG0480022790.01.T01:intron
TACCATTAAAGACGATTTCT+TGG 0.311415 4:+76684991 MsG0480022790.01.T01:CDS
ACAACACATTGATACTTAAC+TGG 0.334226 4:-76682814 None:intergenic
TTTCTCCTTTCAGTTGATTC+CGG 0.335903 4:+76683494 MsG0480022790.01.T01:intron
AGGTGAATTTAAACTGTTGC+AGG 0.336659 4:-76685027 None:intergenic
CACGCAAACTATACGATAGA+AGG 0.344846 4:-76685058 None:intergenic
ACCAACATGATATGAGTATC+AGG 0.401041 4:-76685088 None:intergenic
ACATCAACTAAGTAACTCTA+TGG 0.417740 4:-76685129 None:intergenic
CCATCTGAAGTAGTGTAGTT+TGG 0.431946 4:-76683546 None:intergenic
AAGTGAGGCTAAAGATAATT+GGG 0.441196 4:+76683712 MsG0480022790.01.T01:CDS
AAATATTAGCCATTAATTCT+GGG 0.444034 4:-76685225 None:intergenic
TATAAATAAACTTACATGGT+TGG 0.447507 4:-76683049 None:intergenic
GGATTTGTGGTGGCGGTGAC+GGG 0.462372 4:+76682736 MsG0480022790.01.T01:CDS
TGGATTTGTGGTGGCGGTGA+CGG 0.486501 4:+76682735 MsG0480022790.01.T01:CDS
AGTTTGGCATGTCTCCAGTC+TGG 0.489328 4:-76683530 None:intergenic
GTCTGGAATTCCATATCATC+CGG 0.502025 4:-76683513 None:intergenic
TTGGAAACGAAGATTTGAAC+TGG 0.502780 4:-76683030 None:intergenic
AACTGGAAATGTCACAAATC+CGG 0.505554 4:-76682797 None:intergenic
GGAATTGAGAACATTGGTAA+AGG 0.511677 4:+76682757 MsG0480022790.01.T01:CDS
GGGATAATTCGTGATGGGAC+CGG 0.514333 4:+76682778 MsG0480022790.01.T01:CDS
GAATTGAGAACATTGGTAAA+GGG 0.515497 4:+76682758 MsG0480022790.01.T01:CDS
TACGATAGAAGGGTACATAA+AGG 0.523004 4:-76685047 None:intergenic
CCAAACTACACTACTTCAGA+TGG 0.523620 4:+76683546 MsG0480022790.01.T01:CDS
CTATTACACAGTGGTCGACA+TGG 0.559149 4:+76682715 MsG0480022790.01.T01:CDS
TGTTGTTACCATGGTGAACA+AGG 0.559537 4:+76682870 MsG0480022790.01.T01:CDS
CTACACTACTTCAGATGGAT+CGG 0.562802 4:+76683551 MsG0480022790.01.T01:CDS
CAGTGGTCGACATGGATTTG+TGG 0.563376 4:+76682723 MsG0480022790.01.T01:CDS
GGTGAATTTAAACTGTTGCA+GGG 0.565464 4:-76685026 None:intergenic
CTTAGAAGCTGTTGTTACCA+TGG 0.567172 4:+76682861 MsG0480022790.01.T01:CDS
TTTACAGGTTAAGATTCAGA+AGG 0.579246 4:+76683683 MsG0480022790.01.T01:intron
CTTCTATCGTATAGTTTGCG+TGG 0.589393 4:+76685059 MsG0480022790.01.T01:three_prime_UTR
GGTAAAGGGATAATTCGTGA+TGG 0.598133 4:+76682772 MsG0480022790.01.T01:CDS
GTAAAGGGATAATTCGTGAT+GGG 0.604324 4:+76682773 MsG0480022790.01.T01:CDS
GATGATATGGAATTCCAGAC+TGG 0.604450 4:+76683516 MsG0480022790.01.T01:CDS
AAAGATAATTGGGACTCGAG+TGG 0.614804 4:+76683722 MsG0480022790.01.T01:CDS
ACCTGATACTCATATCATGT+TGG 0.622226 4:+76685087 MsG0480022790.01.T01:three_prime_UTR
TCGACATGGATTTGTGGTGG+CGG 0.622425 4:+76682729 MsG0480022790.01.T01:CDS
ACGCAAACTATACGATAGAA+GGG 0.633204 4:-76685057 None:intergenic
TGCACAAACCTTGTTCACCA+TGG 0.635657 4:-76682878 None:intergenic
GTGACGGGAATTGAGAACAT+TGG 0.640814 4:+76682751 MsG0480022790.01.T01:CDS
TTCAGAAGGATAGTGAAGTG+AGG 0.661943 4:+76683697 MsG0480022790.01.T01:CDS
TGGTCGACATGGATTTGTGG+TGG 0.690678 4:+76682726 MsG0480022790.01.T01:CDS
TAATTCTGGGTACAGCACAA+AGG 0.697158 4:-76685212 None:intergenic
GGCTTTCAACTATTACACAG+TGG 0.715166 4:+76682706 MsG0480022790.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATTATCATAAACTA+AGG - Chr4:76683946-76683965 None:intergenic 10.0%
!!! TAATTTTATTTTTAAGTTCA+TGG + Chr4:76683956-76683975 MsG0480022790.01.T01:intron 10.0%
!!! ATAATTTAGAATAAAACTGA+TGG - Chr4:76683815-76683834 None:intergenic 15.0%
!!! GTTTTATAAATAAACTTACA+TGG - Chr4:76683056-76683075 None:intergenic 15.0%
!!! TAACTATTTATCATGTTTTA+TGG + Chr4:76684901-76684920 MsG0480022790.01.T01:intron 15.0%
!!! TAATTTAGAATAAAACTGAT+GGG - Chr4:76683814-76683833 None:intergenic 15.0%
!!! TTCATATTTGCTTTTATAAT+TGG - Chr4:76684012-76684031 None:intergenic 15.0%
!! AAAATATTAGCCATTAATTC+TGG - Chr4:76685229-76685248 None:intergenic 20.0%
!! AAATATTAGCCATTAATTCT+GGG - Chr4:76685228-76685247 None:intergenic 20.0%
!! AACAATAATAACAGTATCTT+AGG - Chr4:76683098-76683117 None:intergenic 20.0%
!! AACATTATCTAATGCTAAAA+CGG - Chr4:76684703-76684722 None:intergenic 20.0%
!! AGTTAATTAAAACATCTTAC+AGG - Chr4:76684650-76684669 None:intergenic 20.0%
!! AGTTATATACAAAAGATCAA+AGG - Chr4:76684886-76684905 None:intergenic 20.0%
!! ATAAAAGATTAACCTATACT+TGG - Chr4:76684417-76684436 None:intergenic 20.0%
!! ATTTGTACATATCATAAAAG+TGG + Chr4:76684295-76684314 MsG0480022790.01.T01:intron 20.0%
!! TATAAATAAACTTACATGGT+TGG - Chr4:76683052-76683071 None:intergenic 20.0%
!! TGATTCTCATTAAGAAATAT+TGG - Chr4:76684735-76684754 None:intergenic 20.0%
!! TTTAAGAGATTCGAATTAAT+AGG + Chr4:76683838-76683857 MsG0480022790.01.T01:intron 20.0%
!!! AAATGAGAATCATGATTTTA+AGG - Chr4:76683133-76683152 None:intergenic 20.0%
!!! GTTAATCTTTTATCACTTTT+GGG + Chr4:76684424-76684443 MsG0480022790.01.T01:intron 20.0%
!!! TTAATCTTTTATCACTTTTG+GGG + Chr4:76684425-76684444 MsG0480022790.01.T01:intron 20.0%
!!! TTGATTGTTTGTTTTTTTAC+AGG + Chr4:76683668-76683687 MsG0480022790.01.T01:intron 20.0%
!!! TTTATTTTTAAGTTCATGGT+AGG + Chr4:76683960-76683979 MsG0480022790.01.T01:intron 20.0%
! ATAATAATTTCTCGTTGAAG+AGG + Chr4:76683586-76683605 MsG0480022790.01.T01:intron 25.0%
!! AATGTGTTTTCTTCTGTTTT+TGG + Chr4:76683643-76683662 MsG0480022790.01.T01:intron 25.0%
!! CATTTGTCATATTGACTATA+TGG + Chr4:76683293-76683312 MsG0480022790.01.T01:intron 25.0%
!! GGTTAATCTTTTATCACTTT+TGG + Chr4:76684423-76684442 MsG0480022790.01.T01:intron 25.0%
!!! AGAGAGCAATTTGTTTTTTT+AGG + Chr4:76683610-76683629 MsG0480022790.01.T01:intron 25.0%
AAGTGAGGCTAAAGATAATT+GGG + Chr4:76683712-76683731 MsG0480022790.01.T01:CDS 30.0%
ACAACACATTGATACTTAAC+TGG - Chr4:76682817-76682836 None:intergenic 30.0%
ACATCAACTAAGTAACTCTA+TGG - Chr4:76685132-76685151 None:intergenic 30.0%
ACATGAGGAAATAGTAACAA+AGG - Chr4:76683449-76683468 None:intergenic 30.0%
ACTGAAAGCTGCAATATATA+TGG - Chr4:76683768-76683787 None:intergenic 30.0%
ACTTATGTTCTTCTGAATGA+TGG + Chr4:76683245-76683264 MsG0480022790.01.T01:intron 30.0%
AGAAGTAACATCAACAAAGT+AGG - Chr4:76684596-76684615 None:intergenic 30.0%
AGTAAAGTAAAATTGCAGCA+TGG - Chr4:76684479-76684498 None:intergenic 30.0%
AGTGAGGAGAATGAAAAAAT+AGG - Chr4:76684782-76684801 None:intergenic 30.0%
ATCTTAGGTATAAGTGCAAA+AGG - Chr4:76683083-76683102 None:intergenic 30.0%
CAACTGAAAGGAGAAAAAAA+GGG - Chr4:76683490-76683509 None:intergenic 30.0%
CAAGAAACAAAAGCTATATC+TGG - Chr4:76683887-76683906 None:intergenic 30.0%
CTGAAAGCTGCAATATATAT+GGG - Chr4:76683767-76683786 None:intergenic 30.0%
GAATTGAGAACATTGGTAAA+GGG + Chr4:76682758-76682777 MsG0480022790.01.T01:CDS 30.0%
GATCAAGACAAAAATACATG+AGG - Chr4:76683464-76683483 None:intergenic 30.0%
GATCACTATCTGTATGAAAA+TGG + Chr4:76683329-76683348 MsG0480022790.01.T01:intron 30.0%
GTGATAAAAGATTAACCAAG+TGG - Chr4:76684345-76684364 None:intergenic 30.0%
TACCATTAAAGACGATTTCT+TGG + Chr4:76684991-76685010 MsG0480022790.01.T01:CDS 30.0%
TCAACTGAAAGGAGAAAAAA+AGG - Chr4:76683491-76683510 None:intergenic 30.0%
TTCTTGCAAGGTTTGAAAAA+AGG + Chr4:76683363-76683382 MsG0480022790.01.T01:intron 30.0%
TTTACAGGTTAAGATTCAGA+AGG + Chr4:76683683-76683702 MsG0480022790.01.T01:intron 30.0%
!! ATATAGCTTTTGTTTCTTGC+AGG + Chr4:76683888-76683907 MsG0480022790.01.T01:intron 30.0%
!! ATTGTGATATTGAGTTGGAT+AGG + Chr4:76684113-76684132 MsG0480022790.01.T01:intron 30.0%
!! GTTGTTTTCAGACCATTTAA+GGG + Chr4:76682832-76682851 MsG0480022790.01.T01:CDS 30.0%
!! TAATTAACTTGTGATAGCGA+TGG + Chr4:76684661-76684680 MsG0480022790.01.T01:intron 30.0%
!!! GTTTTCATTGATTTCTTGCA+AGG + Chr4:76683351-76683370 MsG0480022790.01.T01:intron 30.0%
!!! TGTTGTTTTCAGACCATTTA+AGG + Chr4:76682831-76682850 MsG0480022790.01.T01:CDS 30.0%
AACTGGAAATGTCACAAATC+CGG - Chr4:76682800-76682819 None:intergenic 35.0%
AAGGCAAAACAGAATAGCTA+CGG - Chr4:76683430-76683449 None:intergenic 35.0%
ACCAACATGATATGAGTATC+AGG - Chr4:76685091-76685110 None:intergenic 35.0%
ACCTGATACTCATATCATGT+TGG + Chr4:76685087-76685106 MsG0480022790.01.T01:three_prime_UTR 35.0%
ACGCAAACTATACGATAGAA+GGG - Chr4:76685060-76685079 None:intergenic 35.0%
AGGAGAATAATAACCCTTCT+CGG - Chr4:76684576-76684595 None:intergenic 35.0%
AGGTGAATTTAAACTGTTGC+AGG - Chr4:76685030-76685049 None:intergenic 35.0%
ATAAACTAAGGCTGTTCTCT+AGG - Chr4:76683934-76683953 None:intergenic 35.0%
ATATGAATAGACCCACTACA+TGG + Chr4:76684025-76684044 MsG0480022790.01.T01:intron 35.0%
ATGAAGTGACTACCAAGTAT+AGG + Chr4:76684402-76684421 MsG0480022790.01.T01:intron 35.0%
CACCAAGAAATCGTCTTTAA+TGG - Chr4:76684996-76685015 None:intergenic 35.0%
CTAATGCTAAAACGGTAACT+AGG - Chr4:76684695-76684714 None:intergenic 35.0%
CTGTATTAAGATGCAGAACA+TGG + Chr4:76684500-76684519 MsG0480022790.01.T01:intron 35.0%
GAAGTGAGGCTAAAGATAAT+TGG + Chr4:76683711-76683730 MsG0480022790.01.T01:CDS 35.0%
GGAATTGAGAACATTGGTAA+AGG + Chr4:76682757-76682776 MsG0480022790.01.T01:CDS 35.0%
GGTGAATTTAAACTGTTGCA+GGG - Chr4:76685029-76685048 None:intergenic 35.0%
GTAAAGGGATAATTCGTGAT+GGG + Chr4:76682773-76682792 MsG0480022790.01.T01:CDS 35.0%
TATCAGTTCATTGCATTCAC+AGG + Chr4:76684259-76684278 MsG0480022790.01.T01:intron 35.0%
TATTCGTGTTATAGCCCTAA+GGG - Chr4:76684807-76684826 None:intergenic 35.0%
TCAGGATTGTGATATTGAGT+TGG + Chr4:76684108-76684127 MsG0480022790.01.T01:intron 35.0%
TCTTACAGGATAATCATGCA+AGG - Chr4:76684636-76684655 None:intergenic 35.0%
TGAGTTCTGTTTGTACAGAT+GGG + Chr4:76682988-76683007 MsG0480022790.01.T01:intron 35.0%
TTGAGTTCTGTTTGTACAGA+TGG + Chr4:76682987-76683006 MsG0480022790.01.T01:intron 35.0%
TTGGAAACGAAGATTTGAAC+TGG - Chr4:76683033-76683052 None:intergenic 35.0%
! TACGATAGAAGGGTACATAA+AGG - Chr4:76685050-76685069 None:intergenic 35.0%
! TATGCTAGTGTTTTACCACT+TGG + Chr4:76684327-76684346 MsG0480022790.01.T01:intron 35.0%
! TCTAAGATCTCTCCCTTAAA+TGG - Chr4:76682847-76682866 None:intergenic 35.0%
! TTTCCTCATCAGCTTTATCT+AGG + Chr4:76682919-76682938 MsG0480022790.01.T01:intron 35.0%
! TTTCTCCTTTCAGTTGATTC+CGG + Chr4:76683494-76683513 MsG0480022790.01.T01:intron 35.0%
!! AATAGGATATGAAAGCACTG+TGG + Chr4:76683855-76683874 MsG0480022790.01.T01:intron 35.0%
!! AGATCTAGCTCTACAATGAA+AGG - Chr4:76684186-76684205 None:intergenic 35.0%
AAAGATAATTGGGACTCGAG+TGG + Chr4:76683722-76683741 MsG0480022790.01.T01:CDS 40.0%
ACACCTAGATAAAGCTGATG+AGG - Chr4:76682925-76682944 None:intergenic 40.0%
AGCTACGGGAGAGAAAAATT+GGG - Chr4:76683415-76683434 None:intergenic 40.0%
AGGCAAAACAGAATAGCTAC+GGG - Chr4:76683429-76683448 None:intergenic 40.0%
ATCATCCGGAATCAACTGAA+AGG - Chr4:76683502-76683521 None:intergenic 40.0%
ATGGTAGGTATACACGAGAA+GGG + Chr4:76684044-76684063 MsG0480022790.01.T01:intron 40.0%
ATGTTGATCCCTGTTGACAT+GGG - Chr4:76684379-76684398 None:intergenic 40.0%
ATTGGATCACCTGTGTCATA+AGG - Chr4:76683994-76684013 None:intergenic 40.0%
CAACTTGACTGCAACAAGTT+TGG - Chr4:76683218-76683237 None:intergenic 40.0%
CACGCAAACTATACGATAGA+AGG - Chr4:76685061-76685080 None:intergenic 40.0%
CCAAACTACACTACTTCAGA+TGG + Chr4:76683546-76683565 MsG0480022790.01.T01:CDS 40.0%
CGAATACAATGAACACTGAC+CGG + Chr4:76684821-76684840 MsG0480022790.01.T01:intron 40.0%
CTACACTACTTCAGATGGAT+CGG + Chr4:76683551-76683570 MsG0480022790.01.T01:CDS 40.0%
CTTAGAAGCTGTTGTTACCA+TGG + Chr4:76682861-76682880 MsG0480022790.01.T01:CDS 40.0%
GAGAGAATATCTCTCTGGTT+AGG + Chr4:76684135-76684154 MsG0480022790.01.T01:intron 40.0%
GAGTTCTGTTTGTACAGATG+GGG + Chr4:76682989-76683008 MsG0480022790.01.T01:intron 40.0%
GATAGGAGAGAATATCTCTC+TGG + Chr4:76684130-76684149 MsG0480022790.01.T01:intron 40.0%
GATGATATGGAATTCCAGAC+TGG + Chr4:76683516-76683535 MsG0480022790.01.T01:CDS 40.0%
GGCTTTCAACTATTACACAG+TGG + Chr4:76682706-76682725 MsG0480022790.01.T01:CDS 40.0%
GGTAAAGGGATAATTCGTGA+TGG + Chr4:76682772-76682791 MsG0480022790.01.T01:CDS 40.0%
GTATTCGTGTTATAGCCCTA+AGG - Chr4:76684808-76684827 None:intergenic 40.0%
GTGTATACCTACCATGTAGT+GGG - Chr4:76684039-76684058 None:intergenic 40.0%
GTTGCATTGCTCATAACTGT+CGG + Chr4:76684232-76684251 MsG0480022790.01.T01:intron 40.0%
TAAGTTCATGGTAGGACCTT+GGG + Chr4:76683968-76683987 MsG0480022790.01.T01:intron 40.0%
TAATTCTGGGTACAGCACAA+AGG - Chr4:76685215-76685234 None:intergenic 40.0%
TAGCTACGGGAGAGAAAAAT+TGG - Chr4:76683416-76683435 None:intergenic 40.0%
TGAGGAAAATGTAGAGACAG+AGG - Chr4:76682907-76682926 None:intergenic 40.0%
TGTGCTGTACCCAGAATTAA+TGG + Chr4:76685216-76685235 MsG0480022790.01.T01:three_prime_UTR 40.0%
TGTGTCGAATGTCGATAAAG+TGG + Chr4:76682955-76682974 MsG0480022790.01.T01:intron 40.0%
TGTTGTTACCATGGTGAACA+AGG + Chr4:76682870-76682889 MsG0480022790.01.T01:CDS 40.0%
TTAAGTTCATGGTAGGACCT+TGG + Chr4:76683967-76683986 MsG0480022790.01.T01:intron 40.0%
TTCAGAAGGATAGTGAAGTG+AGG + Chr4:76683697-76683716 MsG0480022790.01.T01:CDS 40.0%
! AGTTACTTTATGCACCGAGA+AGG + Chr4:76684559-76684578 MsG0480022790.01.T01:intron 40.0%
! CCATCTGAAGTAGTGTAGTT+TGG - Chr4:76683549-76683568 None:intergenic 40.0%
! CTTCTATCGTATAGTTTGCG+TGG + Chr4:76685059-76685078 MsG0480022790.01.T01:three_prime_UTR 40.0%
! GTCTGGAATTCCATATCATC+CGG - Chr4:76683516-76683535 None:intergenic 40.0%
! GTTACTTTATGCACCGAGAA+GGG + Chr4:76684560-76684579 MsG0480022790.01.T01:intron 40.0%
! TCAGTTGATTCCGGATGATA+TGG + Chr4:76683503-76683522 MsG0480022790.01.T01:intron 40.0%
! TTCTCGGTGCATAAAGTAAC+TGG - Chr4:76684560-76684579 None:intergenic 40.0%
AAAACGGTAACTAGGCTGCT+AGG - Chr4:76684687-76684706 None:intergenic 45.0%
CATGGTAGGTATACACGAGA+AGG + Chr4:76684043-76684062 MsG0480022790.01.T01:intron 45.0%
CATGTCAACAGGGATCAACA+TGG + Chr4:76684378-76684397 MsG0480022790.01.T01:intron 45.0%
CATGTTGATCCCTGTTGACA+TGG - Chr4:76684380-76684399 None:intergenic 45.0%
CCTATGCAGAACTGCAAAAG+AGG - Chr4:76684971-76684990 None:intergenic 45.0%
CGTGTATACCTACCATGTAG+TGG - Chr4:76684040-76684059 None:intergenic 45.0%
CTATTACACAGTGGTCGACA+TGG + Chr4:76682715-76682734 MsG0480022790.01.T01:CDS 45.0%
GAATAGACCCACTACATGGT+AGG + Chr4:76684029-76684048 MsG0480022790.01.T01:intron 45.0%
GAGAAACTGAAGAGTTGCAC+CGG - Chr4:76684843-76684862 None:intergenic 45.0%
TCCAACAACCCATGTCAACA+GGG + Chr4:76684368-76684387 MsG0480022790.01.T01:intron 45.0%
TGCACAAACCTTGTTCACCA+TGG - Chr4:76682881-76682900 None:intergenic 45.0%
TGGTAGGTATACACGAGAAG+GGG + Chr4:76684045-76684064 MsG0480022790.01.T01:intron 45.0%
TTCCAACAACCCATGTCAAC+AGG + Chr4:76684367-76684386 MsG0480022790.01.T01:intron 45.0%
! CCTCTTTTGCAGTTCTGCAT+AGG + Chr4:76684968-76684987 MsG0480022790.01.T01:intron 45.0%
! GTGACGGGAATTGAGAACAT+TGG + Chr4:76682751-76682770 MsG0480022790.01.T01:CDS 45.0%
! GTTGACATGGGTTGTTGGAA+TGG - Chr4:76684367-76684386 None:intergenic 45.0%
!!! ATGGGGTTTTTTGCTGAAGC+TGG + Chr4:76683006-76683025 MsG0480022790.01.T01:CDS 45.0%
AGTTTGGCATGTCTCCAGTC+TGG - Chr4:76683533-76683552 None:intergenic 50.0%
ATACACGAGAAGGGGAAGAC+TGG + Chr4:76684053-76684072 MsG0480022790.01.T01:intron 50.0%
ATTCTCCTCACTCGCCCTTA+GGG + Chr4:76684790-76684809 MsG0480022790.01.T01:intron 50.0%
CAGTGGTCGACATGGATTTG+TGG + Chr4:76682723-76682742 MsG0480022790.01.T01:CDS 50.0%
TCGACATGGATTTGTGGTGG+CGG + Chr4:76682729-76682748 MsG0480022790.01.T01:CDS 50.0%
TGGTCGACATGGATTTGTGG+TGG + Chr4:76682726-76682745 MsG0480022790.01.T01:CDS 50.0%
! AAGGGGAAGACTGGTGTTAG+TGG + Chr4:76684062-76684081 MsG0480022790.01.T01:intron 50.0%
! AGGGGAAGACTGGTGTTAGT+GGG + Chr4:76684063-76684082 MsG0480022790.01.T01:intron 50.0%
! GGGATAATTCGTGATGGGAC+CGG + Chr4:76682778-76682797 MsG0480022790.01.T01:CDS 50.0%
! TCCCTGTTGACATGGGTTGT+TGG - Chr4:76684372-76684391 None:intergenic 50.0%
!! GTCTAGGTGTGAGGGTATTC+AGG + Chr4:76684090-76684109 MsG0480022790.01.T01:intron 50.0%
!! TGGTTAGGAGTCACTGACTC+TGG + Chr4:76684150-76684169 MsG0480022790.01.T01:intron 50.0%
CACCTGTGTCATAAGGCCCA+AGG - Chr4:76683987-76684006 None:intergenic 55.0%
CATTCTCCTCACTCGCCCTT+AGG + Chr4:76684789-76684808 MsG0480022790.01.T01:intron 55.0%
GGTGTTAGTGGGGAGAGTCT+AGG + Chr4:76684074-76684093 MsG0480022790.01.T01:intron 55.0%
TATAGCCCTAAGGGCGAGTG+AGG - Chr4:76684798-76684817 None:intergenic 55.0%
TGGGGAGAGTCTAGGTGTGA+GGG + Chr4:76684082-76684101 MsG0480022790.01.T01:intron 55.0%
! GACCTTGGGCCTTATGACAC+AGG + Chr4:76683982-76684001 MsG0480022790.01.T01:intron 55.0%
! GGGGAAGACTGGTGTTAGTG+GGG + Chr4:76684064-76684083 MsG0480022790.01.T01:intron 55.0%
!! TGGATTTGTGGTGGCGGTGA+CGG + Chr4:76682735-76682754 MsG0480022790.01.T01:CDS 55.0%
GTGGGGAGAGTCTAGGTGTG+AGG + Chr4:76684081-76684100 MsG0480022790.01.T01:intron 60.0%
!! GGATTTGTGGTGGCGGTGAC+GGG + Chr4:76682736-76682755 MsG0480022790.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 76682694 76685262 76682694 ID=MsG0480022790.01;Name=MsG0480022790.01
Chr4 mRNA 76682694 76685262 76682694 ID=MsG0480022790.01.T01;Parent=MsG0480022790.01;Name=MsG0480022790.01.T01;_AED=0.47;_eAED=0.49;_QI=0|1|0.8|1|0.75|0.8|5|244|140
Chr4 exon 76682694 76682891 76682694 ID=MsG0480022790.01.T01:exon:10934;Parent=MsG0480022790.01.T01
Chr4 exon 76683006 76683056 76683006 ID=MsG0480022790.01.T01:exon:10935;Parent=MsG0480022790.01.T01
Chr4 exon 76683507 76683572 76683507 ID=MsG0480022790.01.T01:exon:10936;Parent=MsG0480022790.01.T01
Chr4 exon 76683690 76683758 76683690 ID=MsG0480022790.01.T01:exon:10937;Parent=MsG0480022790.01.T01
Chr4 exon 76684980 76685262 76684980 ID=MsG0480022790.01.T01:exon:10938;Parent=MsG0480022790.01.T01
Chr4 CDS 76682694 76682891 76682694 ID=MsG0480022790.01.T01:cds;Parent=MsG0480022790.01.T01
Chr4 CDS 76683006 76683056 76683006 ID=MsG0480022790.01.T01:cds;Parent=MsG0480022790.01.T01
Chr4 CDS 76683507 76683572 76683507 ID=MsG0480022790.01.T01:cds;Parent=MsG0480022790.01.T01
Chr4 CDS 76683690 76683758 76683690 ID=MsG0480022790.01.T01:cds;Parent=MsG0480022790.01.T01
Chr4 CDS 76684980 76685018 76684980 ID=MsG0480022790.01.T01:cds;Parent=MsG0480022790.01.T01
Chr4 three_prime_UTR 76685019 76685262 76685019 ID=MsG0480022790.01.T01:three_prime_utr;Parent=MsG0480022790.01.T01
Gene Sequence

>MsG0480022790.01.T01

ATGCAACTTTTGGGCTTTCAACTATTACACAGTGGTCGACATGGATTTGTGGTGGCGGTGACGGGAATTGAGAACATTGGTAAAGGGATAATTCGTGATGGGACCGGATTTGTGACATTTCCAGTTAAGTATCAATGTGTTGTTTTCAGACCATTTAAGGGAGAGATCTTAGAAGCTGTTGTTACCATGGTGAACAAGATGGGGTTTTTTGCTGAAGCTGGACCAGTTCAAATCTTCGTTTCCAACCATTTGATTCCGGATGATATGGAATTCCAGACTGGAGACATGCCAAACTACACTACTTCAGATGGATCGGTTAAGATTCAGAAGGATAGTGAAGTGAGGCTAAAGATAATTGGGACTCGAGTGGATGCTACTGAAATTTTCTGCATAGGTACCATTAAAGACGATTTCTTGGTGTGA
Protein sequence

>MsG0480022790.01.T01

MQLLGFQLLHSGRHGFVVAVTGIENIGKGIIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQTGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLV*