Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035588.01.T01 | AEV52286.2 | 62.5 | 168 | 34 | 5 | 13 | 151 | 110 | 277 | 5.66E-28 | 115 |
| MsG0680035588.01.T01 | AEV52286.2 | 46.471 | 170 | 23 | 5 | 17 | 118 | 114 | 283 | 8.39E-13 | 75.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035588.01.T01 | P28641 | 46.067 | 89 | 22 | 2 | 18 | 80 | 144 | 232 | 6.15E-13 | 67 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035588.01.T01 | G9FYZ2 | 62.500 | 168 | 34 | 5 | 13 | 151 | 110 | 277 | 2.70e-28 | 115 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680035588.01 | MsG0680035598.01 | 0.862562 | 4.641125e-64 | 1.365039e-60 |
| MsG0680035588.01 | MsG0880043829.01 | 0.854125 | 1.515923e-61 | 3.351536e-58 |
| MsG0680035588.01 | MsG0880046536.01 | 0.849435 | 3.240049e-60 | 6.141555e-57 |
| MsG0680035588.01 | MsG0880046930.01 | 0.804362 | 2.239861e-49 | 1.152654e-46 |
| MsG0480021776.01 | MsG0680035588.01 | -0.811383 | 7.195246e-51 | 4.447384e-48 |
| MsG0480022243.01 | MsG0680035588.01 | 0.834012 | 3.833499e-56 | 4.491426e-53 |
| MsG0480022245.01 | MsG0680035588.01 | 0.837237 | 5.852549e-57 | 7.554706e-54 |
| MsG0480022246.01 | MsG0680035588.01 | 0.812991 | 3.208598e-51 | 2.070076e-48 |
| MsG0480023652.01 | MsG0680035588.01 | 0.801251 | 9.831859e-49 | 4.673369e-46 |
| MsG0580024597.01 | MsG0680035588.01 | 0.814269 | 1.679232e-51 | 1.121614e-48 |
| MsG0580025280.01 | MsG0680035588.01 | 0.838442 | 2.868204e-57 | 3.842335e-54 |
| MsG0380017022.01 | MsG0680035588.01 | 0.835173 | 1.957885e-56 | 2.375170e-53 |
| MsG0380017023.01 | MsG0680035588.01 | 0.852338 | 4.928011e-61 | 1.026478e-57 |
| MsG0380017791.01 | MsG0680035588.01 | 0.803827 | 2.895338e-49 | 1.469587e-46 |
| MsG0680030636.01 | MsG0680035588.01 | 0.801190 | 1.012220e-48 | 4.803823e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035588.01.T01 | MTR_6g084640 | 62.353 | 170 | 31 | 6 | 17 | 154 | 144 | 312 | 1.89e-30 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 64 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGCTGCTAGTACTTA+TGG | 0.182311 | 6:-108867872 | MsG0680035588.01.T01:CDS |
| GGAGCTACCGGTGGCGGAAC+TGG | 0.275507 | 6:-108867950 | MsG0680035588.01.T01:CDS |
| AGGGGTTGTGGACAAGATTA+AGG | 0.278783 | 6:-108867681 | MsG0680035588.01.T01:CDS |
| AGGGATTGTGGACAAGATTA+AGG | 0.287150 | 6:-108867564 | MsG0680035588.01.T01:CDS |
| AAGATTAAGGAGAAGCTTCC+TGG | 0.308792 | 6:-108867551 | MsG0680035588.01.T01:CDS |
| AAGATTCCTGGCACTGGAAC+TGG | 0.323822 | 6:-108867656 | MsG0680035588.01.T01:CDS |
| CCTGGCACTGGAACTGGAAC+TGG | 0.334665 | 6:-108867650 | MsG0680035588.01.T01:CDS |
| CCTGTTCCAGTTCCAGTTCC+AGG | 0.353061 | 6:+108867761 | None:intergenic |
| AAGATTCCTGGAACTGGAAC+TGG | 0.359093 | 6:-108867767 | MsG0680035588.01.T01:CDS |
| TCTCAATATCAACAAGGTTA+TGG | 0.374924 | 6:-108867971 | MsG0680035588.01.T01:CDS |
| AGGGGTAATGGACAAGATTA+AGG | 0.379804 | 6:-108867915 | MsG0680035588.01.T01:CDS |
| AAGATCAAGGAGAAGATTCC+TGG | 0.387597 | 6:-108867779 | MsG0680035588.01.T01:CDS |
| CCTGGAACTGGAACTGGAAC+AGG | 0.387624 | 6:-108867761 | MsG0680035588.01.T01:CDS |
| ACTGGAACTGGAACTGGAAC+AGG | 0.409631 | 6:-108867644 | MsG0680035588.01.T01:CDS |
| AAGATTAAGGCGAAAATTCC+CGG | 0.410456 | 6:-108867902 | MsG0680035588.01.T01:CDS |
| AAGATTAAGGAGAAGATTCC+TGG | 0.417229 | 6:-108867668 | MsG0680035588.01.T01:CDS |
| AAAATGCTGCTAGTACTTAT+GGG | 0.425979 | 6:-108867871 | MsG0680035588.01.T01:CDS |
| AAGGAGAAGATTCCTGGAAC+TGG | 0.429498 | 6:-108867773 | MsG0680035588.01.T01:CDS |
| TGGAGATAATAGAGGAGTTA+TGG | 0.444393 | 6:-108867804 | MsG0680035588.01.T01:CDS |
| CATCAACAACATGGAAAGAA+AGG | 0.456958 | 6:-108867584 | MsG0680035588.01.T01:CDS |
| CATCAACAACATGGAAAGAA+AGG | 0.456958 | 6:-108867701 | MsG0680035588.01.T01:CDS |
| GGAACAGGTCATGGAACAAC+TGG | 0.460620 | 6:-108867629 | MsG0680035588.01.T01:CDS |
| GGAACAGGTCATGGAACAAC+TGG | 0.460620 | 6:-108867746 | MsG0680035588.01.T01:CDS |
| GGGACAGGACATGGAACAAC+TGG | 0.471797 | 6:-108867845 | MsG0680035588.01.T01:CDS |
| GGTCATGGAACAACTGGTTA+TGG | 0.473670 | 6:-108867623 | MsG0680035588.01.T01:CDS |
| GGTCATGGAACAACTGGTTA+TGG | 0.473670 | 6:-108867740 | MsG0680035588.01.T01:CDS |
| CCAGTTCCAGTTCCAGTGCC+AGG | 0.475451 | 6:+108867650 | None:intergenic |
| GAGCTACCGGTGGCGGAACT+GGG | 0.485254 | 6:-108867949 | MsG0680035588.01.T01:CDS |
| GCATGATAATAGAGGGGTAA+TGG | 0.494411 | 6:-108867927 | MsG0680035588.01.T01:CDS |
| GGTTATGGTGCTGGTGGTCA+TGG | 0.495776 | 6:-108867725 | MsG0680035588.01.T01:CDS |
| AGGAGTTATGGACAAGATCA+AGG | 0.502550 | 6:-108867792 | MsG0680035588.01.T01:CDS |
| TAGTGTCCTTGTCCATGTCC+AGG | 0.504026 | 6:+108867533 | None:intergenic |
| AGTACTTATGGGACAGGGAC+AGG | 0.509114 | 6:-108867860 | MsG0680035588.01.T01:CDS |
| ATCAACAACATGGAAAGAAA+GGG | 0.511347 | 6:-108867700 | MsG0680035588.01.T01:CDS |
| GCTGCTAGTACTTATGGGAC+AGG | 0.515021 | 6:-108867866 | MsG0680035588.01.T01:CDS |
| GGTTATGGAGCTACCGGTGG+CGG | 0.520450 | 6:-108867956 | MsG0680035588.01.T01:CDS |
| ACATGGAAAGAAAGGGGTTG+TGG | 0.523511 | 6:-108867693 | MsG0680035588.01.T01:CDS |
| ACTGGAACTGGAACAGGTCA+TGG | 0.523949 | 6:-108867638 | MsG0680035588.01.T01:CDS |
| ACTGGAACTGGAACAGGTCA+TGG | 0.523949 | 6:-108867755 | MsG0680035588.01.T01:CDS |
| GGAACTGGGCATGATAATAG+AGG | 0.529404 | 6:-108867935 | MsG0680035588.01.T01:CDS |
| AAGGAGAAGATTCCTGGCAC+TGG | 0.533139 | 6:-108867662 | MsG0680035588.01.T01:CDS |
| CAACAAGGTTATGGAGCTAC+CGG | 0.539969 | 6:-108867962 | MsG0680035588.01.T01:CDS |
| GGAACAACTGGTTATGGTGC+TGG | 0.541736 | 6:-108867734 | MsG0680035588.01.T01:CDS |
| GGTTATGGTGGTGTTGGTCA+TGG | 0.545868 | 6:-108867608 | MsG0680035588.01.T01:CDS |
| ATCAACAACATGGAAAGAAA+GGG | 0.547780 | 6:-108867583 | MsG0680035588.01.T01:CDS |
| CAACAACATGGAGATAATAG+AGG | 0.548264 | 6:-108867812 | MsG0680035588.01.T01:CDS |
| ATCATGCCCAGTTCCGCCAC+CGG | 0.554198 | 6:+108867943 | None:intergenic |
| AAGGAGAAGCTTCCTGGACA+TGG | 0.566266 | 6:-108867545 | MsG0680035588.01.T01:CDS |
| TATGGGACAGGGACAGGACA+TGG | 0.569049 | 6:-108867854 | MsG0680035588.01.T01:CDS |
| ACATGGAAAGAAAGGGATTG+TGG | 0.578076 | 6:-108867576 | MsG0680035588.01.T01:CDS |
| GGACATGGAACAACTGGTGT+TGG | 0.586251 | 6:-108867839 | MsG0680035588.01.T01:CDS |
| ACAACTGGTTATGGTGGTGT+TGG | 0.599005 | 6:-108867614 | MsG0680035588.01.T01:CDS |
| AAGCTTCCTGGACATGGACA+AGG | 0.605612 | 6:-108867539 | MsG0680035588.01.T01:CDS |
| GAACTGGGCATGATAATAGA+GGG | 0.607970 | 6:-108867934 | MsG0680035588.01.T01:CDS |
| CTGCTAGTACTTATGGGACA+GGG | 0.654209 | 6:-108867865 | MsG0680035588.01.T01:CDS |
| GGTCATGGTCATCAACAACA+TGG | 0.654732 | 6:-108867710 | MsG0680035588.01.T01:CDS |
| TCAACAACATGGAAAGAAAG+GGG | 0.658215 | 6:-108867699 | MsG0680035588.01.T01:CDS |
| CAAGGTTATGGAGCTACCGG+TGG | 0.670970 | 6:-108867959 | MsG0680035588.01.T01:CDS |
| GGTGTTGGTCATCAACAACA+TGG | 0.679464 | 6:-108867824 | MsG0680035588.01.T01:CDS |
| ACAACTGGTTATGGTGCTGG+TGG | 0.680489 | 6:-108867731 | MsG0680035588.01.T01:CDS |
| GGTCATGGTCATCAACAACA+TGG | 0.689366 | 6:-108867593 | MsG0680035588.01.T01:CDS |
| AACTGGGCATGATAATAGAG+GGG | 0.696526 | 6:-108867933 | MsG0680035588.01.T01:CDS |
| CATGGAACAACTGGTTATGG+TGG | 0.707484 | 6:-108867620 | MsG0680035588.01.T01:CDS |
| ACAATGTCTCAATATCAACA+AGG | 0.721195 | 6:-108867977 | MsG0680035588.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGCTGCTAGTACTTAT+GGG | - | Chr6:108867635-108867654 | MsG0680035588.01.T01:CDS | 30.0% | |
| ATCAACAACATGGAAAGAAA+GGG | - | Chr6:108867806-108867825 | MsG0680035588.01.T01:CDS | 30.0% | |
| TCTCAATATCAACAAGGTTA+TGG | - | Chr6:108867535-108867554 | MsG0680035588.01.T01:CDS | 30.0% | |
| AAGATTAAGGAGAAGATTCC+TGG | - | Chr6:108867838-108867857 | MsG0680035588.01.T01:CDS | 35.0% | |
| AAGATTAAGGCGAAAATTCC+CGG | - | Chr6:108867604-108867623 | MsG0680035588.01.T01:CDS | 35.0% | |
| CAAAATGCTGCTAGTACTTA+TGG | - | Chr6:108867634-108867653 | MsG0680035588.01.T01:CDS | 35.0% | |
| CAACAACATGGAGATAATAG+AGG | - | Chr6:108867694-108867713 | MsG0680035588.01.T01:CDS | 35.0% | |
| CATCAACAACATGGAAAGAA+AGG | - | Chr6:108867805-108867824 | MsG0680035588.01.T01:CDS | 35.0% | |
| TCAACAACATGGAAAGAAAG+GGG | - | Chr6:108867807-108867826 | MsG0680035588.01.T01:CDS | 35.0% | |
| TGGAGATAATAGAGGAGTTA+TGG | - | Chr6:108867702-108867721 | MsG0680035588.01.T01:CDS | 35.0% | |
| AACTGGGCATGATAATAGAG+GGG | - | Chr6:108867573-108867592 | MsG0680035588.01.T01:CDS | 40.0% | |
| AAGATCAAGGAGAAGATTCC+TGG | - | Chr6:108867727-108867746 | MsG0680035588.01.T01:CDS | 40.0% | |
| AAGATTAAGGAGAAGCTTCC+TGG | - | Chr6:108867955-108867974 | MsG0680035588.01.T01:CDS | 40.0% | |
| ACATGGAAAGAAAGGGATTG+TGG | - | Chr6:108867930-108867949 | MsG0680035588.01.T01:CDS | 40.0% | |
| AGGAGTTATGGACAAGATCA+AGG | - | Chr6:108867714-108867733 | MsG0680035588.01.T01:CDS | 40.0% | |
| AGGGATTGTGGACAAGATTA+AGG | - | Chr6:108867942-108867961 | MsG0680035588.01.T01:CDS | 40.0% | |
| GAACTGGGCATGATAATAGA+GGG | - | Chr6:108867572-108867591 | MsG0680035588.01.T01:CDS | 40.0% | |
| GCATGATAATAGAGGGGTAA+TGG | - | Chr6:108867579-108867598 | MsG0680035588.01.T01:CDS | 40.0% | |
| !! | AGCAGCATTTTGATGAGTAC+CGG | + | Chr6:108867626-108867645 | None:intergenic | 40.0% |
| !! | AGGGGTAATGGACAAGATTA+AGG | - | Chr6:108867591-108867610 | MsG0680035588.01.T01:CDS | 40.0% |
| AAGATTCCTGGAACTGGAAC+TGG | - | Chr6:108867739-108867758 | MsG0680035588.01.T01:CDS | 45.0% | |
| AAGGAGAAGATTCCTGGAAC+TGG | - | Chr6:108867733-108867752 | MsG0680035588.01.T01:CDS | 45.0% | |
| ACATGGAAAGAAAGGGGTTG+TGG | - | Chr6:108867813-108867832 | MsG0680035588.01.T01:CDS | 45.0% | |
| AGGGGTTGTGGACAAGATTA+AGG | - | Chr6:108867825-108867844 | MsG0680035588.01.T01:CDS | 45.0% | |
| CAACAAGGTTATGGAGCTAC+CGG | - | Chr6:108867544-108867563 | MsG0680035588.01.T01:CDS | 45.0% | |
| CATGGAACAACTGGTTATGG+TGG | - | Chr6:108867769-108867788 | MsG0680035588.01.T01:CDS | 45.0% | |
| CTGCTAGTACTTATGGGACA+GGG | - | Chr6:108867641-108867660 | MsG0680035588.01.T01:CDS | 45.0% | |
| GGAACTGGGCATGATAATAG+AGG | - | Chr6:108867571-108867590 | MsG0680035588.01.T01:CDS | 45.0% | |
| GGTCATGGAACAACTGGTTA+TGG | - | Chr6:108867766-108867785 | MsG0680035588.01.T01:CDS | 45.0% | |
| GGTCATGGTCATCAACAACA+TGG | - | Chr6:108867796-108867815 | MsG0680035588.01.T01:CDS | 45.0% | |
| ! | ACAACTGGTTATGGTGGTGT+TGG | - | Chr6:108867892-108867911 | MsG0680035588.01.T01:CDS | 45.0% |
| !! | GCAGCATTTTGATGAGTACC+GGG | + | Chr6:108867625-108867644 | None:intergenic | 45.0% |
| !! | GGTGTTGGTCATCAACAACA+TGG | - | Chr6:108867682-108867701 | MsG0680035588.01.T01:CDS | 45.0% |
| AAGCTTCCTGGACATGGACA+AGG | - | Chr6:108867967-108867986 | MsG0680035588.01.T01:CDS | 50.0% | |
| AAGGAGAAGATTCCTGGCAC+TGG | - | Chr6:108867844-108867863 | MsG0680035588.01.T01:CDS | 50.0% | |
| AAGGAGAAGCTTCCTGGACA+TGG | - | Chr6:108867961-108867980 | MsG0680035588.01.T01:CDS | 50.0% | |
| ACTGGAACTGGAACAGGTCA+TGG | - | Chr6:108867751-108867770 | MsG0680035588.01.T01:CDS | 50.0% | |
| ACTGGAACTGGAACTGGAAC+AGG | - | Chr6:108867862-108867881 | MsG0680035588.01.T01:CDS | 50.0% | |
| AGTACTTATGGGACAGGGAC+AGG | - | Chr6:108867646-108867665 | MsG0680035588.01.T01:CDS | 50.0% | |
| GCTGCTAGTACTTATGGGAC+AGG | - | Chr6:108867640-108867659 | MsG0680035588.01.T01:CDS | 50.0% | |
| GGAACAACTGGTTATGGTGC+TGG | - | Chr6:108867772-108867791 | MsG0680035588.01.T01:CDS | 50.0% | |
| GGAACAGGTCATGGAACAAC+TGG | - | Chr6:108867760-108867779 | MsG0680035588.01.T01:CDS | 50.0% | |
| GGACATGGAACAACTGGTGT+TGG | - | Chr6:108867667-108867686 | MsG0680035588.01.T01:CDS | 50.0% | |
| TAGTGTCCTTGTCCATGTCC+AGG | + | Chr6:108867976-108867995 | None:intergenic | 50.0% | |
| ! | AAGATTCCTGGCACTGGAAC+TGG | - | Chr6:108867850-108867869 | MsG0680035588.01.T01:CDS | 50.0% |
| ! | ACAACTGGTTATGGTGCTGG+TGG | - | Chr6:108867775-108867794 | MsG0680035588.01.T01:CDS | 50.0% |
| !! | GGTTATGGTGGTGTTGGTCA+TGG | - | Chr6:108867898-108867917 | MsG0680035588.01.T01:CDS | 50.0% |
| CAAGGTTATGGAGCTACCGG+TGG | - | Chr6:108867547-108867566 | MsG0680035588.01.T01:CDS | 55.0% | |
| CCTGGAACTGGAACTGGAAC+AGG | - | Chr6:108867745-108867764 | MsG0680035588.01.T01:CDS | 55.0% | |
| CCTGTTCCAGTTCCAGTTCC+AGG | + | Chr6:108867748-108867767 | None:intergenic | 55.0% | |
| GGGACAGGACATGGAACAAC+TGG | - | Chr6:108867661-108867680 | MsG0680035588.01.T01:CDS | 55.0% | |
| TATGGGACAGGGACAGGACA+TGG | - | Chr6:108867652-108867671 | MsG0680035588.01.T01:CDS | 55.0% | |
| !! | GGTTATGGTGCTGGTGGTCA+TGG | - | Chr6:108867781-108867800 | MsG0680035588.01.T01:CDS | 55.0% |
| ATCATGCCCAGTTCCGCCAC+CGG | + | Chr6:108867566-108867585 | None:intergenic | 60.0% | |
| CCAGTTCCAGTTCCAGTGCC+AGG | + | Chr6:108867859-108867878 | None:intergenic | 60.0% | |
| GGTTATGGAGCTACCGGTGG+CGG | - | Chr6:108867550-108867569 | MsG0680035588.01.T01:CDS | 60.0% | |
| ! | CCTGGCACTGGAACTGGAAC+TGG | - | Chr6:108867856-108867875 | MsG0680035588.01.T01:CDS | 60.0% |
| !! | GAGCTACCGGTGGCGGAACT+GGG | - | Chr6:108867557-108867576 | MsG0680035588.01.T01:CDS | 65.0% |
| !! | GGAGCTACCGGTGGCGGAAC+TGG | - | Chr6:108867556-108867575 | MsG0680035588.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 108867532 | 108867996 | 108867532 | ID=MsG0680035588.01;Name=MsG0680035588.01 |
| Chr6 | mRNA | 108867532 | 108867996 | 108867532 | ID=MsG0680035588.01.T01;Parent=MsG0680035588.01;Name=MsG0680035588.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|154 |
| Chr6 | exon | 108867532 | 108867996 | 108867532 | ID=MsG0680035588.01.T01:exon:34796;Parent=MsG0680035588.01.T01 |
| Chr6 | CDS | 108867532 | 108867996 | 108867532 | ID=MsG0680035588.01.T01:cds;Parent=MsG0680035588.01.T01 |
| Gene Sequence |
| Protein sequence |
