Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022246.01.T01 | GAU32879.1 | 77.907 | 86 | 19 | 0 | 1 | 86 | 1 | 86 | 1.16E-37 | 131 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022246.01.T01 | A0A2Z6NMK5 | 77.907 | 86 | 19 | 0 | 1 | 86 | 1 | 86 | 5.54e-38 | 131 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048843.01 | MsG0480022246.01 | 0.807024 | 6.186173e-50 | 3.408716e-47 |
| MsG0080049011.01 | MsG0480022246.01 | 0.822650 | 2.115049e-53 | 1.781075e-50 |
| MsG0180000157.01 | MsG0480022246.01 | 0.803506 | 3.374228e-49 | 1.698667e-46 |
| MsG0180000271.01 | MsG0480022246.01 | 0.838744 | 2.397230e-57 | 3.240900e-54 |
| MsG0180000363.01 | MsG0480022246.01 | 0.815312 | 9.862434e-52 | 6.776010e-49 |
| MsG0180000611.01 | MsG0480022246.01 | 0.827048 | 1.939524e-54 | 1.851169e-51 |
| MsG0180001576.01 | MsG0480022246.01 | -0.803493 | 3.395490e-49 | 1.708759e-46 |
| MsG0180001712.01 | MsG0480022246.01 | 0.810655 | 1.034938e-50 | 6.274713e-48 |
| MsG0180002140.01 | MsG0480022246.01 | 0.842244 | 2.910841e-58 | 4.386492e-55 |
| MsG0180002750.01 | MsG0480022246.01 | -0.803307 | 3.710210e-49 | 1.858161e-46 |
| MsG0180003118.01 | MsG0480022246.01 | -0.806655 | 7.401169e-50 | 4.039281e-47 |
| MsG0180003379.01 | MsG0480022246.01 | 0.861169 | 1.239298e-63 | 3.474019e-60 |
| MsG0180004016.01 | MsG0480022246.01 | 0.805282 | 1.439382e-49 | 7.584333e-47 |
| MsG0180004684.01 | MsG0480022246.01 | 0.845671 | 3.510515e-59 | 5.894901e-56 |
| MsG0180005106.01 | MsG0480022246.01 | 0.867133 | 1.712813e-65 | 5.914621e-62 |
| MsG0180005268.01 | MsG0480022246.01 | 0.842220 | 2.953628e-58 | 4.447539e-55 |
| MsG0180005270.01 | MsG0480022246.01 | 0.817937 | 2.544993e-52 | 1.878903e-49 |
| MsG0180005354.01 | MsG0480022246.01 | 0.800507 | 1.395621e-48 | 6.509575e-46 |
| MsG0180005382.01 | MsG0480022246.01 | 0.804877 | 1.749553e-49 | 9.124415e-47 |
| MsG0180005446.01 | MsG0480022246.01 | 0.853466 | 2.345798e-61 | 5.073515e-58 |
| MsG0180005509.01 | MsG0480022246.01 | 0.813156 | 2.952402e-51 | 1.913129e-48 |
| MsG0180005787.01 | MsG0480022246.01 | 0.831614 | 1.510791e-55 | 1.648142e-52 |
| MsG0180006046.01 | MsG0480022246.01 | 0.857869 | 1.216792e-62 | 3.046741e-59 |
| MsG0280011073.01 | MsG0480022246.01 | 0.807784 | 4.267840e-50 | 2.398604e-47 |
| MsG0280011287.01 | MsG0480022246.01 | 0.885068 | 1.117504e-71 | 7.560507e-68 |
| MsG0280011371.01 | MsG0480022246.01 | 0.810259 | 1.259707e-50 | 7.557722e-48 |
| MsG0280011376.01 | MsG0480022246.01 | 0.805569 | 1.253031e-49 | 6.651382e-47 |
| MsG0380013471.01 | MsG0480022246.01 | 0.826971 | 2.024056e-54 | 1.927631e-51 |
| MsG0380013853.01 | MsG0480022246.01 | 0.805679 | 1.188395e-49 | 6.326439e-47 |
| MsG0480018847.01 | MsG0480022246.01 | 0.827306 | 1.682983e-54 | 1.618575e-51 |
| MsG0480018906.01 | MsG0480022246.01 | 0.802635 | 5.109929e-49 | 2.515657e-46 |
| MsG0480020327.01 | MsG0480022246.01 | -0.806541 | 7.822797e-50 | 4.257322e-47 |
| MsG0480020843.01 | MsG0480022246.01 | 0.820459 | 6.782760e-53 | 5.370213e-50 |
| MsG0480021490.01 | MsG0480022246.01 | -0.816294 | 5.957242e-52 | 4.204832e-49 |
| MsG0480021776.01 | MsG0480022246.01 | -0.827351 | 1.641171e-54 | 1.580604e-51 |
| MsG0480021876.01 | MsG0480022246.01 | -0.808071 | 3.707787e-50 | 2.099603e-47 |
| MsG0480022239.01 | MsG0480022246.01 | 0.829235 | 5.767465e-55 | 5.868177e-52 |
| MsG0480022243.01 | MsG0480022246.01 | 0.884101 | 2.555858e-71 | 1.664472e-67 |
| MsG0480022245.01 | MsG0480022246.01 | 0.891821 | 2.797554e-74 | 2.491588e-70 |
| MsG0480022246.01 | MsG0480022248.01 | 0.902082 | 1.393331e-78 | 1.939682e-74 |
| MsG0480022246.01 | MsG0480022493.01 | 0.805232 | 1.474336e-49 | 7.758798e-47 |
| MsG0480022246.01 | MsG0480023136.01 | -0.817029 | 4.076589e-52 | 2.935768e-49 |
| MsG0480022246.01 | MsG0580024084.01 | 0.840281 | 9.557920e-58 | 1.354813e-54 |
| MsG0480022246.01 | MsG0580024581.01 | 0.806292 | 8.830820e-50 | 4.775352e-47 |
| MsG0480022246.01 | MsG0580024597.01 | 0.817559 | 3.096626e-52 | 2.262621e-49 |
| MsG0480022246.01 | MsG0680030555.01 | 0.819282 | 1.260278e-52 | 9.655777e-50 |
| MsG0480022246.01 | MsG0680031446.01 | -0.802422 | 5.653409e-49 | 2.768309e-46 |
| MsG0480022246.01 | MsG0680031536.01 | 0.817407 | 3.351100e-52 | 2.438441e-49 |
| MsG0480022246.01 | MsG0680032510.01 | -0.825960 | 3.524844e-54 | 3.261065e-51 |
| MsG0480022246.01 | MsG0680032516.01 | 0.809303 | 2.022386e-50 | 1.183155e-47 |
| MsG0480022246.01 | MsG0680034655.01 | 0.802423 | 5.650032e-49 | 2.766745e-46 |
| MsG0480022246.01 | MsG0680035330.01 | -0.807677 | 4.497362e-50 | 2.520534e-47 |
| MsG0480022246.01 | MsG0680035588.01 | 0.812991 | 3.208598e-51 | 2.070076e-48 |
| MsG0480022246.01 | MsG0680035598.01 | 0.819971 | 8.774269e-53 | 6.853655e-50 |
| MsG0480022246.01 | MsG0780036117.01 | 0.801701 | 7.953915e-49 | 3.823647e-46 |
| MsG0480022246.01 | MsG0780037082.01 | 0.816264 | 6.049460e-52 | 4.266384e-49 |
| MsG0480022246.01 | MsG0780040208.01 | 0.863921 | 1.762300e-64 | 5.433650e-61 |
| MsG0480022246.01 | MsG0780040375.01 | -0.855462 | 6.206925e-62 | 1.434275e-58 |
| MsG0480022246.01 | MsG0780040424.01 | 0.837258 | 5.780862e-57 | 7.466919e-54 |
| MsG0480022246.01 | MsG0780040686.01 | 0.830715 | 2.512961e-55 | 2.669323e-52 |
| MsG0480022246.01 | MsG0780041729.01 | -0.817147 | 3.834309e-52 | 2.770149e-49 |
| MsG0480022246.01 | MsG0880043194.01 | 0.806058 | 9.891916e-50 | 5.317411e-47 |
| MsG0480022246.01 | MsG0880043829.01 | 0.806427 | 8.269648e-50 | 4.487309e-47 |
| MsG0480022246.01 | MsG0880044062.01 | -0.806637 | 7.467180e-50 | 4.073414e-47 |
| MsG0480022246.01 | MsG0880044524.01 | 0.811294 | 7.522232e-51 | 4.638449e-48 |
| MsG0480022246.01 | MsG0880046377.01 | 0.837418 | 5.260742e-57 | 6.829160e-54 |
| MsG0480022246.01 | MsG0880046877.01 | -0.805110 | 1.563296e-49 | 8.201358e-47 |
| MsG0480022246.01 | MsG0880047152.01 | 0.814611 | 1.410885e-51 | 9.511206e-49 |
| MsG0380015818.01 | MsG0480022246.01 | 0.812554 | 3.998473e-51 | 2.550167e-48 |
| MsG0380016117.01 | MsG0480022246.01 | 0.843385 | 1.447422e-58 | 2.261080e-55 |
| MsG0380016118.01 | MsG0480022246.01 | -0.812516 | 4.076554e-51 | 2.597323e-48 |
| MsG0380016182.01 | MsG0480022246.01 | 0.801117 | 1.047289e-48 | 4.961344e-46 |
| MsG0380016740.01 | MsG0480022246.01 | 0.824845 | 6.471815e-54 | 5.800375e-51 |
| MsG0380017022.01 | MsG0480022246.01 | 0.853438 | 2.389583e-61 | 5.163550e-58 |
| MsG0380017023.01 | MsG0480022246.01 | 0.875890 | 2.156887e-68 | 1.024561e-64 |
| MsG0380017085.01 | MsG0480022246.01 | 0.811501 | 6.785021e-51 | 4.207117e-48 |
| MsG0380017215.01 | MsG0480022246.01 | 0.803586 | 3.248660e-49 | 1.638817e-46 |
| MsG0380017265.01 | MsG0480022246.01 | -0.820954 | 5.221379e-53 | 4.191443e-50 |
| MsG0380017279.01 | MsG0480022246.01 | 0.835770 | 1.382809e-56 | 1.707854e-53 |
| MsG0380017512.01 | MsG0480022246.01 | 0.867361 | 1.448267e-65 | 5.043110e-62 |
| MsG0380017791.01 | MsG0480022246.01 | 0.849886 | 2.424176e-60 | 4.663477e-57 |
| MsG0480018244.01 | MsG0480022246.01 | -0.813003 | 3.189447e-51 | 2.058377e-48 |
| MsG0480018292.01 | MsG0480022246.01 | 0.855026 | 8.313309e-62 | 1.893123e-58 |
| MsG0480018402.01 | MsG0480022246.01 | 0.805270 | 1.447713e-49 | 7.625973e-47 |
| MsG0280006501.01 | MsG0480022246.01 | 0.828239 | 1.003897e-54 | 9.920753e-52 |
| MsG0280006634.01 | MsG0480022246.01 | 0.801165 | 1.024209e-48 | 4.857757e-46 |
| MsG0280006669.01 | MsG0480022246.01 | 0.813594 | 2.365749e-51 | 1.551514e-48 |
| MsG0280007165.01 | MsG0480022246.01 | -0.826898 | 2.106807e-54 | 2.002155e-51 |
| MsG0280007743.01 | MsG0480022246.01 | -0.826347 | 2.852069e-54 | 2.667867e-51 |
| MsG0280007775.01 | MsG0480022246.01 | 0.818492 | 1.905801e-52 | 1.428710e-49 |
| MsG0280007776.01 | MsG0480022246.01 | 0.835548 | 1.573563e-56 | 1.930524e-53 |
| MsG0280009350.01 | MsG0480022246.01 | 0.811503 | 6.778611e-51 | 4.203352e-48 |
| MsG0280009554.01 | MsG0480022246.01 | 0.810393 | 1.178629e-50 | 7.095934e-48 |
| MsG0280009676.01 | MsG0480022246.01 | -0.819773 | 9.740811e-53 | 7.566655e-50 |
| MsG0280010204.01 | MsG0480022246.01 | 0.809615 | 1.733458e-50 | 1.022660e-47 |
| MsG0280010205.01 | MsG0480022246.01 | 0.825718 | 4.024200e-54 | 3.697340e-51 |
| MsG0280010423.01 | MsG0480022246.01 | -0.804741 | 1.867189e-49 | 9.703725e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022246.01.T01 | MTR_4g082883 | 52.000 | 75 | 31 | 2 | 16 | 86 | 26 | 99 | 8.85e-14 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTTGGTAGAAACTTATT+TGG | 0.193374 | 4:+70077720 | None:intergenic |
| GTTTCTACCAAGTGAGGTTT+TGG | 0.234806 | 4:-70077712 | MsG0480022246.01.T01:CDS |
| AGCACACCACCTTCGGATTT+AGG | 0.251846 | 4:+70077540 | None:intergenic |
| GAGAAGGTGTCAACTGCTGC+AGG | 0.400905 | 4:-70077636 | MsG0480022246.01.T01:CDS |
| TCCGAAGGTGGTGTGCTTGA+TGG | 0.441201 | 4:-70077534 | MsG0480022246.01.T01:CDS |
| GAGTCAGCACAGACAACTGC+AGG | 0.472436 | 4:-70077669 | MsG0480022246.01.T01:CDS |
| ACCATCAAGCACACCACCTT+CGG | 0.487811 | 4:+70077533 | None:intergenic |
| CTGTAGTTGAGCATGTTGAC+AGG | 0.490478 | 4:-70077599 | MsG0480022246.01.T01:CDS |
| TCGGATTTAGGAGGCTCCTC+TGG | 0.493494 | 4:+70077552 | None:intergenic |
| GCACTCCCCAAAACCTCACT+TGG | 0.541506 | 4:+70077705 | None:intergenic |
| AAAGAAACAATGGCAGAAAG+TGG | 0.543749 | 4:-70077747 | None:intergenic |
| AAATAAGTTTCTACCAAGTG+AGG | 0.567992 | 4:-70077718 | MsG0480022246.01.T01:CDS |
| GGTGTCAACTGCTGCAGGGG+AGG | 0.571411 | 4:-70077631 | MsG0480022246.01.T01:CDS |
| TTTAGGAGGCTCCTCTGGTG+TGG | 0.573361 | 4:+70077557 | None:intergenic |
| AGAAGGTGTCAACTGCTGCA+GGG | 0.573757 | 4:-70077635 | MsG0480022246.01.T01:CDS |
| GAAGGTGTCAACTGCTGCAG+GGG | 0.582580 | 4:-70077634 | MsG0480022246.01.T01:CDS |
| ACACCACCTTCGGATTTAGG+AGG | 0.610944 | 4:+70077543 | None:intergenic |
| TGCAGGGAAACAATCTGAGA+AGG | 0.618025 | 4:-70077652 | MsG0480022246.01.T01:CDS |
| GAGGAGCCTCCTAAATCCGA+AGG | 0.645422 | 4:-70077549 | MsG0480022246.01.T01:CDS |
| TCATGTTCATTCCACACCAG+AGG | 0.674914 | 4:-70077568 | MsG0480022246.01.T01:CDS |
| GAGCCTCCTAAATCCGAAGG+TGG | 0.678300 | 4:-70077546 | MsG0480022246.01.T01:CDS |
| TGTAGTTGAGCATGTTGACA+GGG | 0.688044 | 4:-70077598 | MsG0480022246.01.T01:CDS |
| AGTCAGCACAGACAACTGCA+GGG | 0.705798 | 4:-70077668 | MsG0480022246.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAAGTTTCTACCAAGTG+AGG | - | Chr4:70077520-70077539 | MsG0480022246.01.T01:CDS | 30.0% | |
| TCACTTGGTAGAAACTTATT+TGG | + | Chr4:70077521-70077540 | None:intergenic | 30.0% | |
| ! | GATGGTGTTAAAAAAGCTGT+TGG | - | Chr4:70077722-70077741 | MsG0480022246.01.T01:CDS | 35.0% |
| ! | TTTCTACCAAGTGAGGTTTT+GGG | - | Chr4:70077527-70077546 | MsG0480022246.01.T01:CDS | 35.0% |
| GGTGTTAAAAAAGCTGTTGG+AGG | - | Chr4:70077725-70077744 | MsG0480022246.01.T01:CDS | 40.0% | |
| TGTAGTTGAGCATGTTGACA+GGG | - | Chr4:70077640-70077659 | MsG0480022246.01.T01:CDS | 40.0% | |
| ! | GTTTCTACCAAGTGAGGTTT+TGG | - | Chr4:70077526-70077545 | MsG0480022246.01.T01:CDS | 40.0% |
| ! | TTCTACCAAGTGAGGTTTTG+GGG | - | Chr4:70077528-70077547 | MsG0480022246.01.T01:CDS | 40.0% |
| CTGTAGTTGAGCATGTTGAC+AGG | - | Chr4:70077639-70077658 | MsG0480022246.01.T01:CDS | 45.0% | |
| TCATGTTCATTCCACACCAG+AGG | - | Chr4:70077670-70077689 | MsG0480022246.01.T01:CDS | 45.0% | |
| TGCAGGGAAACAATCTGAGA+AGG | - | Chr4:70077586-70077605 | MsG0480022246.01.T01:CDS | 45.0% | |
| ACACCACCTTCGGATTTAGG+AGG | + | Chr4:70077698-70077717 | None:intergenic | 50.0% | |
| ACCATCAAGCACACCACCTT+CGG | + | Chr4:70077708-70077727 | None:intergenic | 50.0% | |
| AGCACACCACCTTCGGATTT+AGG | + | Chr4:70077701-70077720 | None:intergenic | 50.0% | |
| AGTCAGCACAGACAACTGCA+GGG | - | Chr4:70077570-70077589 | MsG0480022246.01.T01:CDS | 50.0% | |
| !! | AGAAGGTGTCAACTGCTGCA+GGG | - | Chr4:70077603-70077622 | MsG0480022246.01.T01:CDS | 50.0% |
| !!! | TGAGGTTTTGGGGAGTGCAA+AGG | - | Chr4:70077538-70077557 | MsG0480022246.01.T01:CDS | 50.0% |
| GAGCCTCCTAAATCCGAAGG+TGG | - | Chr4:70077692-70077711 | MsG0480022246.01.T01:CDS | 55.0% | |
| GAGGAGCCTCCTAAATCCGA+AGG | - | Chr4:70077689-70077708 | MsG0480022246.01.T01:CDS | 55.0% | |
| GAGTCAGCACAGACAACTGC+AGG | - | Chr4:70077569-70077588 | MsG0480022246.01.T01:CDS | 55.0% | |
| GCACTCCCCAAAACCTCACT+TGG | + | Chr4:70077536-70077555 | None:intergenic | 55.0% | |
| TCCGAAGGTGGTGTGCTTGA+TGG | - | Chr4:70077704-70077723 | MsG0480022246.01.T01:CDS | 55.0% | |
| TCGGATTTAGGAGGCTCCTC+TGG | + | Chr4:70077689-70077708 | None:intergenic | 55.0% | |
| TTTAGGAGGCTCCTCTGGTG+TGG | + | Chr4:70077684-70077703 | None:intergenic | 55.0% | |
| !! | GAAGGTGTCAACTGCTGCAG+GGG | - | Chr4:70077604-70077623 | MsG0480022246.01.T01:CDS | 55.0% |
| !! | GAGAAGGTGTCAACTGCTGC+AGG | - | Chr4:70077602-70077621 | MsG0480022246.01.T01:CDS | 55.0% |
| !!! | GGTTTTGGGGAGTGCAAAGG+TGG | - | Chr4:70077541-70077560 | MsG0480022246.01.T01:CDS | 55.0% |
| GGTGTCAACTGCTGCAGGGG+AGG | - | Chr4:70077607-70077626 | MsG0480022246.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 70077500 | 70077760 | 70077500 | ID=MsG0480022246.01;Name=MsG0480022246.01 |
| Chr4 | mRNA | 70077500 | 70077760 | 70077500 | ID=MsG0480022246.01.T01;Parent=MsG0480022246.01;Name=MsG0480022246.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|86 |
| Chr4 | exon | 70077500 | 70077760 | 70077500 | ID=MsG0480022246.01.T01:exon:11102;Parent=MsG0480022246.01.T01 |
| Chr4 | CDS | 70077500 | 70077760 | 70077500 | ID=MsG0480022246.01.T01:cds;Parent=MsG0480022246.01.T01 |
| Gene Sequence |
| Protein sequence |
