Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036591.01.T01 | AES77692.2 | 91.703 | 458 | 38 | 0 | 1 | 458 | 1 | 458 | 0 | 874 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036591.01.T01 | Q9LRQ8 | 39.619 | 472 | 243 | 13 | 4 | 458 | 5 | 451 | 2.79E-97 | 302 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036591.01.T01 | G7L664 | 91.703 | 458 | 38 | 0 | 1 | 458 | 1 | 458 | 0.0 | 874 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001565.01 | MsG0780036591.01 | 0.805764 | 1.140582e-49 | 6.085307e-47 |
| MsG0180002071.01 | MsG0780036591.01 | 0.801574 | 8.443356e-49 | 4.046121e-46 |
| MsG0180002952.01 | MsG0780036591.01 | 0.804397 | 2.203179e-49 | 1.134730e-46 |
| MsG0180004357.01 | MsG0780036591.01 | 0.800199 | 1.612100e-48 | 7.460777e-46 |
| MsG0280010614.01 | MsG0780036591.01 | 0.809020 | 2.326275e-50 | 1.350586e-47 |
| MsG0280011282.01 | MsG0780036591.01 | 0.809972 | 1.453013e-50 | 8.652346e-48 |
| MsG0280011285.01 | MsG0780036591.01 | 0.817185 | 3.760098e-52 | 2.719314e-49 |
| MsG0280011293.01 | MsG0780036591.01 | 0.800828 | 1.200071e-48 | 5.643324e-46 |
| MsG0380014573.01 | MsG0780036591.01 | 0.808203 | 3.475245e-50 | 1.974943e-47 |
| MsG0480020465.01 | MsG0780036591.01 | 0.815821 | 7.595899e-52 | 5.292162e-49 |
| MsG0480020634.01 | MsG0780036591.01 | 0.802978 | 4.340653e-49 | 2.155617e-46 |
| MsG0580024157.01 | MsG0780036591.01 | 0.806038 | 9.986284e-50 | 5.365313e-47 |
| MsG0580025532.01 | MsG0780036591.01 | 0.800271 | 1.558445e-48 | 7.225917e-46 |
| MsG0680030672.01 | MsG0780036591.01 | 0.801638 | 8.191297e-49 | 3.931618e-46 |
| MsG0280009514.01 | MsG0780036591.01 | 0.819433 | 1.164429e-52 | 8.959426e-50 |
| MsG0780036018.01 | MsG0780036591.01 | 0.809345 | 1.981003e-50 | 1.160274e-47 |
| MsG0780036591.01 | MsG0780038602.01 | 0.821571 | 3.762787e-53 | 3.072591e-50 |
| MsG0780036591.01 | MsG0780038691.01 | 0.801542 | 8.572435e-49 | 4.104589e-46 |
| MsG0780036591.01 | MsG0780039059.01 | 0.816815 | 4.553256e-52 | 3.259801e-49 |
| MsG0780036591.01 | MsG0780039252.01 | 0.811602 | 6.450505e-51 | 4.009988e-48 |
| MsG0780036591.01 | MsG0780039655.01 | 0.836978 | 6.814597e-57 | 8.728572e-54 |
| MsG0780036591.01 | MsG0780040488.01 | 0.815278 | 1.003529e-51 | 6.888529e-49 |
| MsG0780036591.01 | MsG0780040669.01 | 0.803840 | 2.876605e-49 | 1.460625e-46 |
| MsG0780036591.01 | MsG0880046479.01 | 0.814114 | 1.816784e-51 | 1.208356e-48 |
| MsG0780036591.01 | MsG0880046669.01 | 0.800092 | 1.694628e-48 | 7.821648e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036591.01.T01 | MTR_7g014330 | 91.703 | 458 | 38 | 0 | 1 | 458 | 1 | 458 | 0.0 | 874 |
| MsG0780036591.01.T01 | MTR_7g014230 | 91.189 | 454 | 40 | 0 | 2 | 455 | 4 | 457 | 0.0 | 855 |
| MsG0780036591.01.T01 | MTR_7g014250 | 89.520 | 458 | 48 | 0 | 1 | 458 | 1 | 458 | 0.0 | 855 |
| MsG0780036591.01.T01 | MTR_7g014360 | 83.247 | 388 | 45 | 1 | 71 | 458 | 21 | 388 | 0.0 | 660 |
| MsG0780036591.01.T01 | MTR_7g014310 | 70.359 | 334 | 45 | 2 | 108 | 441 | 29 | 308 | 5.11e-158 | 449 |
| MsG0780036591.01.T01 | MTR_6g015815 | 47.425 | 466 | 225 | 9 | 2 | 458 | 8 | 462 | 3.23e-148 | 431 |
| MsG0780036591.01.T01 | MTR_6g015850 | 47.198 | 464 | 224 | 10 | 3 | 458 | 9 | 459 | 7.08e-145 | 422 |
| MsG0780036591.01.T01 | MTR_6g015830 | 47.323 | 467 | 222 | 11 | 3 | 458 | 9 | 462 | 6.17e-144 | 420 |
| MsG0780036591.01.T01 | MTR_6g015320 | 46.436 | 463 | 226 | 11 | 3 | 458 | 9 | 456 | 1.07e-139 | 409 |
| MsG0780036591.01.T01 | MTR_6g015315 | 45.770 | 461 | 233 | 9 | 3 | 458 | 6 | 454 | 4.23e-137 | 402 |
| MsG0780036591.01.T01 | MTR_7g014610 | 41.474 | 475 | 253 | 11 | 3 | 458 | 9 | 477 | 1.79e-112 | 340 |
| MsG0780036591.01.T01 | MTR_7g014200 | 40.598 | 468 | 259 | 11 | 3 | 458 | 10 | 470 | 1.50e-109 | 332 |
| MsG0780036591.01.T01 | MTR_7g014160 | 40.336 | 476 | 252 | 15 | 3 | 457 | 10 | 474 | 3.88e-105 | 321 |
| MsG0780036591.01.T01 | MTR_7g013850 | 37.879 | 462 | 246 | 14 | 27 | 457 | 30 | 481 | 7.52e-95 | 295 |
| MsG0780036591.01.T01 | MTR_7g014620 | 38.789 | 446 | 238 | 9 | 22 | 456 | 11 | 432 | 2.80e-92 | 286 |
| MsG0780036591.01.T01 | MTR_2g089765 | 36.975 | 476 | 260 | 13 | 3 | 452 | 17 | 478 | 3.52e-85 | 270 |
| MsG0780036591.01.T01 | MTR_3g028210 | 34.820 | 471 | 275 | 13 | 3 | 456 | 9 | 464 | 1.16e-83 | 265 |
| MsG0780036591.01.T01 | MTR_7g015860 | 35.158 | 475 | 259 | 11 | 3 | 452 | 6 | 456 | 2.80e-79 | 254 |
| MsG0780036591.01.T01 | MTR_2g089670 | 37.251 | 451 | 245 | 16 | 22 | 452 | 41 | 473 | 2.23e-78 | 252 |
| MsG0780036591.01.T01 | MTR_6g015310 | 39.394 | 363 | 163 | 8 | 74 | 433 | 23 | 331 | 5.15e-78 | 246 |
| MsG0780036591.01.T01 | MTR_8g012750 | 34.591 | 477 | 281 | 12 | 3 | 456 | 4 | 472 | 5.60e-78 | 251 |
| MsG0780036591.01.T01 | MTR_3g087640 | 35.417 | 480 | 267 | 13 | 3 | 456 | 10 | 472 | 5.19e-75 | 243 |
| MsG0780036591.01.T01 | MTR_2g089735 | 34.744 | 449 | 240 | 14 | 39 | 458 | 39 | 463 | 4.08e-71 | 233 |
| MsG0780036591.01.T01 | MTR_2g089650 | 33.747 | 483 | 266 | 16 | 3 | 458 | 10 | 465 | 1.49e-70 | 231 |
| MsG0780036591.01.T01 | MTR_2g089755 | 33.187 | 455 | 268 | 13 | 26 | 458 | 37 | 477 | 1.60e-68 | 226 |
| MsG0780036591.01.T01 | MTR_7g014630 | 42.446 | 278 | 139 | 7 | 3 | 267 | 10 | 279 | 5.42e-66 | 216 |
| MsG0780036591.01.T01 | MTR_7g014205 | 75.573 | 131 | 21 | 1 | 295 | 425 | 1 | 120 | 5.95e-66 | 207 |
| MsG0780036591.01.T01 | MTR_7g014210 | 81.739 | 115 | 20 | 1 | 314 | 427 | 24 | 138 | 4.00e-58 | 189 |
| MsG0780036591.01.T01 | MTR_2g089665 | 30.995 | 442 | 186 | 11 | 1 | 431 | 1 | 334 | 4.51e-52 | 179 |
| MsG0780036591.01.T01 | MTR_3g435300 | 37.956 | 274 | 142 | 8 | 3 | 262 | 4 | 263 | 2.36e-45 | 159 |
| MsG0780036591.01.T01 | MTR_7g014180 | 46.746 | 169 | 84 | 2 | 3 | 168 | 8 | 173 | 7.79e-43 | 149 |
| MsG0780036591.01.T01 | MTR_7g014340 | 87.324 | 71 | 9 | 0 | 1 | 71 | 1 | 71 | 5.77e-36 | 128 |
| MsG0780036591.01.T01 | MTR_7g014670 | 37.368 | 190 | 113 | 4 | 272 | 456 | 71 | 259 | 1.13e-33 | 127 |
| MsG0780036591.01.T01 | MTR_7g014220 | 86.885 | 61 | 8 | 0 | 14 | 74 | 6 | 66 | 9.29e-29 | 108 |
| MsG0780036591.01.T01 | MTR_3g049150 | 27.027 | 407 | 229 | 16 | 34 | 409 | 30 | 399 | 3.47e-19 | 90.1 |
| MsG0780036591.01.T01 | MTR_3g088420 | 26.761 | 426 | 225 | 17 | 20 | 413 | 18 | 388 | 6.60e-19 | 89.0 |
| MsG0780036591.01.T01 | MTR_3g026310 | 26.376 | 436 | 242 | 21 | 11 | 413 | 14 | 403 | 6.62e-19 | 89.4 |
| MsG0780036591.01.T01 | MTR_2g064495 | 23.448 | 435 | 265 | 16 | 42 | 452 | 39 | 429 | 8.50e-19 | 88.6 |
| MsG0780036591.01.T01 | MTR_8g075610 | 25.191 | 393 | 221 | 17 | 42 | 409 | 30 | 374 | 9.63e-19 | 88.6 |
| MsG0780036591.01.T01 | MTR_4g022825 | 26.190 | 420 | 242 | 15 | 20 | 413 | 18 | 395 | 1.46e-18 | 88.2 |
| MsG0780036591.01.T01 | MTR_0190s0020 | 26.906 | 446 | 239 | 20 | 23 | 437 | 26 | 415 | 2.35e-18 | 87.4 |
| MsG0780036591.01.T01 | MTR_7g026860 | 25.926 | 405 | 252 | 14 | 20 | 409 | 18 | 389 | 5.17e-18 | 86.3 |
| MsG0780036591.01.T01 | MTR_2g105330 | 23.448 | 435 | 265 | 16 | 42 | 452 | 39 | 429 | 6.13e-18 | 86.3 |
| MsG0780036591.01.T01 | MTR_3g101900 | 24.510 | 408 | 249 | 12 | 26 | 412 | 30 | 399 | 7.15e-18 | 86.3 |
| MsG0780036591.01.T01 | MTR_4g094842 | 27.078 | 421 | 250 | 19 | 26 | 427 | 27 | 409 | 8.34e-18 | 85.9 |
| MsG0780036591.01.T01 | MTR_7g085930 | 24.886 | 438 | 289 | 11 | 43 | 456 | 40 | 461 | 1.72e-17 | 85.1 |
| MsG0780036591.01.T01 | MTR_3g026950 | 25.450 | 444 | 252 | 17 | 32 | 433 | 21 | 427 | 4.28e-17 | 83.6 |
| MsG0780036591.01.T01 | MTR_4g022800 | 27.578 | 417 | 242 | 17 | 20 | 413 | 18 | 397 | 8.60e-16 | 79.7 |
| MsG0780036591.01.T01 | MTR_8g096530 | 25.634 | 355 | 228 | 12 | 62 | 410 | 59 | 383 | 1.00e-15 | 79.3 |
| MsG0780036591.01.T01 | MTR_3g101930 | 26.854 | 391 | 221 | 16 | 48 | 412 | 56 | 407 | 1.18e-15 | 79.3 |
| MsG0780036591.01.T01 | MTR_3g098980 | 24.205 | 409 | 252 | 14 | 62 | 453 | 66 | 433 | 4.77e-15 | 77.4 |
| MsG0780036591.01.T01 | MTR_4g094828 | 25.854 | 410 | 258 | 16 | 20 | 413 | 23 | 402 | 5.13e-15 | 77.4 |
| MsG0780036591.01.T01 | MTR_7g085990 | 25.225 | 333 | 216 | 7 | 43 | 362 | 36 | 348 | 1.89e-14 | 75.5 |
| MsG0780036591.01.T01 | MTR_7g086000 | 25.625 | 480 | 302 | 19 | 4 | 456 | 6 | 457 | 7.08e-14 | 73.9 |
| MsG0780036591.01.T01 | MTR_4g022720 | 25.000 | 408 | 257 | 14 | 20 | 413 | 18 | 390 | 8.06e-13 | 70.5 |
| MsG0780036591.01.T01 | MTR_4g127650 | 23.593 | 462 | 290 | 18 | 24 | 456 | 29 | 456 | 1.31e-12 | 69.7 |
| MsG0780036591.01.T01 | MTR_3g435290 | 34.483 | 116 | 71 | 3 | 344 | 458 | 8 | 119 | 3.99e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036591.01.T01 | AT3G29670 | 39.619 | 472 | 243 | 13 | 4 | 458 | 5 | 451 | 2.85e-98 | 302 |
| MsG0780036591.01.T01 | AT5G39090 | 38.229 | 463 | 252 | 11 | 5 | 456 | 8 | 447 | 5.87e-96 | 296 |
| MsG0780036591.01.T01 | AT5G39080 | 38.065 | 465 | 258 | 12 | 4 | 452 | 7 | 457 | 5.73e-95 | 294 |
| MsG0780036591.01.T01 | AT5G39050 | 38.771 | 472 | 256 | 13 | 3 | 456 | 11 | 467 | 9.12e-95 | 294 |
| MsG0780036591.01.T01 | AT3G29635 | 40.127 | 471 | 245 | 14 | 3 | 456 | 4 | 454 | 1.67e-93 | 290 |
| MsG0780036591.01.T01 | AT5G61160 | 37.263 | 475 | 253 | 14 | 3 | 458 | 4 | 452 | 8.64e-90 | 281 |
| MsG0780036591.01.T01 | AT3G29680 | 40.000 | 460 | 244 | 14 | 4 | 452 | 5 | 443 | 2.83e-89 | 279 |
| MsG0780036591.01.T01 | AT3G29590 | 38.166 | 469 | 244 | 15 | 4 | 456 | 10 | 448 | 1.49e-88 | 277 |
| MsG0780036591.01.T01 | AT1G03940 | 34.167 | 480 | 266 | 17 | 3 | 456 | 9 | 464 | 4.45e-77 | 248 |
| MsG0780036591.01.T01 | AT1G03495 | 35.465 | 344 | 188 | 12 | 4 | 331 | 10 | 335 | 3.11e-54 | 185 |
| MsG0780036591.01.T01 | AT3G29720 | 38.647 | 207 | 107 | 5 | 99 | 299 | 26 | 218 | 2.06e-31 | 120 |
| MsG0780036591.01.T01 | AT5G23940 | 25.223 | 448 | 253 | 17 | 26 | 436 | 25 | 427 | 9.55e-21 | 95.1 |
| MsG0780036591.01.T01 | AT2G19070 | 23.094 | 446 | 284 | 12 | 39 | 456 | 37 | 451 | 1.20e-20 | 94.7 |
| MsG0780036591.01.T01 | AT3G29690 | 34.868 | 152 | 79 | 6 | 312 | 455 | 38 | 177 | 1.11e-16 | 78.2 |
| MsG0780036591.01.T01 | AT3G47170 | 25.532 | 282 | 174 | 9 | 62 | 328 | 70 | 330 | 2.64e-15 | 78.2 |
| MsG0780036591.01.T01 | AT5G48930 | 23.059 | 425 | 272 | 16 | 42 | 448 | 39 | 426 | 3.55e-15 | 77.8 |
| MsG0780036591.01.T01 | AT5G67160 | 23.373 | 415 | 258 | 10 | 10 | 409 | 13 | 382 | 8.85e-15 | 76.6 |
| MsG0780036591.01.T01 | AT5G07850 | 24.324 | 407 | 257 | 12 | 26 | 413 | 31 | 405 | 4.86e-14 | 74.3 |
| MsG0780036591.01.T01 | AT2G39980 | 23.947 | 451 | 274 | 18 | 4 | 413 | 10 | 432 | 1.05e-13 | 73.6 |
| MsG0780036591.01.T01 | AT1G31490 | 23.881 | 402 | 247 | 15 | 23 | 409 | 26 | 383 | 1.00e-12 | 70.1 |
| MsG0780036591.01.T01 | AT5G38130 | 25.829 | 422 | 241 | 20 | 29 | 425 | 52 | 426 | 1.44e-12 | 69.7 |
| MsG0780036591.01.T01 | AT1G24430 | 21.739 | 460 | 291 | 13 | 6 | 450 | 11 | 416 | 7.41e-12 | 67.4 |
| MsG0780036591.01.T01 | AT1G28680 | 23.611 | 288 | 184 | 6 | 62 | 342 | 62 | 320 | 1.16e-11 | 67.0 |
| MsG0780036591.01.T01 | AT1G28680 | 23.529 | 289 | 185 | 6 | 62 | 343 | 62 | 321 | 1.17e-11 | 66.6 |
| MsG0780036591.01.T01 | AT3G48720 | 23.219 | 379 | 248 | 15 | 39 | 409 | 41 | 384 | 1.67e-11 | 66.2 |
Find 77 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGATTCCTACGACATATTT+TGG | 0.085486 | 7:-10185136 | MsG0780036591.01.T01:CDS |
| TTTGAAACAAGTGAATTATT+TGG | 0.202070 | 7:-10185085 | MsG0780036591.01.T01:CDS |
| AGTGAATTATTTGGAGAAAA+TGG | 0.206523 | 7:-10185076 | MsG0780036591.01.T01:CDS |
| GCTGATTTCAATCATTTATC+TGG | 0.253026 | 7:-10185772 | MsG0780036591.01.T01:CDS |
| GGAAAGTGATTGTTTGAGTT+TGG | 0.268451 | 7:+10185915 | None:intergenic |
| GAAACCATTTCAACTTTCTT+TGG | 0.269632 | 7:+10184869 | None:intergenic |
| GTACTATAAACCTCAAATTT+AGG | 0.281913 | 7:+10184911 | None:intergenic |
| TAACTTTCTTTGACATACTA+TGG | 0.286472 | 7:-10186017 | MsG0780036591.01.T01:CDS |
| ACATGATCAAGCTTCTGTTT+TGG | 0.289012 | 7:-10185692 | MsG0780036591.01.T01:CDS |
| CCAATTGAAAATCCATAGTT+TGG | 0.297340 | 7:+10185649 | None:intergenic |
| AACCAACAATAGAAGCTTAA+TGG | 0.299594 | 7:-10185410 | MsG0780036591.01.T01:CDS |
| AGGCAAAATATTTGAGTGAT+TGG | 0.316985 | 7:-10185447 | MsG0780036591.01.T01:CDS |
| CCCTTGATGAATTTGATGAT+TGG | 0.320166 | 7:+10185829 | None:intergenic |
| GTTATTTCAATTATCTCGTT+TGG | 0.320907 | 7:-10185350 | MsG0780036591.01.T01:CDS |
| GTTCCAAATGATTCATTTAG+AGG | 0.320989 | 7:-10185373 | MsG0780036591.01.T01:CDS |
| ACAATAGAAGCTTAATGGTT+TGG | 0.327026 | 7:-10185405 | MsG0780036591.01.T01:CDS |
| AACCATTAAGCTTCTATTGT+TGG | 0.335300 | 7:+10185408 | None:intergenic |
| CAATAGAAGCTTAATGGTTT+GGG | 0.346870 | 7:-10185404 | MsG0780036591.01.T01:CDS |
| GAGGTTTATAGTACTGATTT+TGG | 0.352559 | 7:-10184902 | MsG0780036591.01.T01:CDS |
| GAGCTGAAAATTGGTCTTCA+TGG | 0.367514 | 7:-10184988 | MsG0780036591.01.T01:CDS |
| TTTGGAGATCGAAAAGCTTA+AGG | 0.373057 | 7:-10185332 | MsG0780036591.01.T01:CDS |
| ATTGGTTTGTGAGAATCATT+AGG | 0.380290 | 7:+10185847 | None:intergenic |
| TGCTGGTTCACCTAAATTTG+AGG | 0.384622 | 7:-10184921 | MsG0780036591.01.T01:CDS |
| CAAGGATCCAAATGAATTAG+AGG | 0.386216 | 7:-10185467 | MsG0780036591.01.T01:CDS |
| TCTTCACTTGTCAACATTTG+TGG | 0.387843 | 7:-10185263 | MsG0780036591.01.T01:CDS |
| AGTATGTCAAAGAAAGTTAG+AGG | 0.388038 | 7:+10186021 | None:intergenic |
| AACCCTCTAAATGAATCATT+TGG | 0.391425 | 7:+10185370 | None:intergenic |
| TCATGTGAAATATTCAAGTT+AGG | 0.394263 | 7:+10185709 | None:intergenic |
| ATTACCGATGTTAAAGCAAT+TGG | 0.395582 | 7:-10184947 | MsG0780036591.01.T01:CDS |
| ATTGTTTGAGTTTGGGAAGA+AGG | 0.398368 | 7:+10185923 | None:intergenic |
| TAGAAGAAAATTCTTTGAAC+AGG | 0.399183 | 7:+10185982 | None:intergenic |
| ATTTGTAGTGTCAAAACAAA+AGG | 0.406667 | 7:-10185308 | MsG0780036591.01.T01:CDS |
| GAAAGTGATTGTTTGAGTTT+GGG | 0.409790 | 7:+10185916 | None:intergenic |
| CCAAAATATGTCGTAGGAAT+CGG | 0.410672 | 7:+10185136 | None:intergenic |
| TGTCTTTCTGACTGTAGAAA+TGG | 0.418931 | 7:-10184824 | MsG0780036591.01.T01:CDS |
| TGAAGTGTTGAGTGAAGCTT+TGG | 0.425405 | 7:-10185038 | MsG0780036591.01.T01:CDS |
| TGGTCTTCATGGTTACTTGA+AGG | 0.428251 | 7:-10184977 | MsG0780036591.01.T01:CDS |
| TGGCTTTGAATATCGATTGC+CGG | 0.431534 | 7:-10185174 | MsG0780036591.01.T01:CDS |
| AAAACCAAAGAAAGTTGAAA+TGG | 0.434697 | 7:-10184873 | MsG0780036591.01.T01:CDS |
| AATGGTGATGGTGTTGAGAT+AGG | 0.443549 | 7:-10184806 | MsG0780036591.01.T01:CDS |
| AATTGCTTTAACATCGGTAA+TGG | 0.446233 | 7:+10184949 | None:intergenic |
| TGTTGAATGGAGCTGAAAAT+TGG | 0.446827 | 7:-10184997 | MsG0780036591.01.T01:CDS |
| TCGGCTGATCTAAACGATTC+CGG | 0.447477 | 7:+10185155 | None:intergenic |
| AGAGGAAGTGAGGTTTCTGA+AGG | 0.472786 | 7:+10186039 | None:intergenic |
| TTGTTTGAGTTTGGGAAGAA+GGG | 0.473939 | 7:+10185924 | None:intergenic |
| ATAAAGAATGGAGTGTTGAA+TGG | 0.475613 | 7:-10185010 | MsG0780036591.01.T01:CDS |
| ACCAATCATCAAATTCATCA+AGG | 0.476412 | 7:-10185830 | MsG0780036591.01.T01:CDS |
| AAATTCATGTGGCAAACAAA+GGG | 0.476984 | 7:+10185516 | None:intergenic |
| ATGGTTTCTATAGATAGAAC+TGG | 0.490163 | 7:-10184854 | MsG0780036591.01.T01:CDS |
| TATTGAAGAAATGTGAGGTT+TGG | 0.494665 | 7:+10185947 | None:intergenic |
| AAAGAAAGTTAGAGGAAGTG+AGG | 0.497044 | 7:+10186029 | None:intergenic |
| TGTCAAAACAAAAGGGTACT+AGG | 0.501108 | 7:-10185300 | MsG0780036591.01.T01:CDS |
| AAGAAAATTCTTTGAACAGG+TGG | 0.502827 | 7:+10185985 | None:intergenic |
| TTTGCTTCGCTCTTTGTCAA+AGG | 0.503257 | 7:-10184752 | MsG0780036591.01.T01:CDS |
| GTTAAAGCAATTGGTGCTGC+TGG | 0.503742 | 7:-10184938 | MsG0780036591.01.T01:CDS |
| TTCCAAATGATTCATTTAGA+GGG | 0.515033 | 7:-10185372 | MsG0780036591.01.T01:CDS |
| GGTAGTTCTTCTTGTGAAGA+TGG | 0.518675 | 7:+10186060 | None:intergenic |
| CTATGATAGAAAAGTAATCA+AGG | 0.519078 | 7:-10185485 | MsG0780036591.01.T01:CDS |
| GGTATCTATTGCTTATGCAT+TGG | 0.520850 | 7:-10185242 | MsG0780036591.01.T01:CDS |
| AAAATTCATGTGGCAAACAA+AGG | 0.522274 | 7:+10185515 | None:intergenic |
| TTGAGTGATTGGTTAAAGCA+AGG | 0.525666 | 7:-10185436 | MsG0780036591.01.T01:CDS |
| GCTTTGGAAACTATAAAGAA+TGG | 0.532473 | 7:-10185022 | MsG0780036591.01.T01:CDS |
| AGCACCAATTGCTTTAACAT+CGG | 0.552039 | 7:+10184943 | None:intergenic |
| TCTACAGTCAGAAAGACAAA+AGG | 0.555841 | 7:+10184829 | None:intergenic |
| TTTGTAGTGTCAAAACAAAA+GGG | 0.558518 | 7:-10185307 | MsG0780036591.01.T01:CDS |
| CAAATGAGATGACCTAAAAG+AGG | 0.571110 | 7:+10185871 | None:intergenic |
| TGGAGAAAATGGACTAATTG+TGG | 0.575913 | 7:-10185065 | MsG0780036591.01.T01:CDS |
| CCAATCATCAAATTCATCAA+GGG | 0.585572 | 7:-10185829 | MsG0780036591.01.T01:CDS |
| CAATTTCCAAAATATGTCGT+AGG | 0.610979 | 7:+10185130 | None:intergenic |
| AGTGATTGGTTAAAGCAAGG+TGG | 0.623091 | 7:-10185433 | MsG0780036591.01.T01:CDS |
| ATAAAAGTGAGAAAGTGCAA+TGG | 0.624999 | 7:+10185894 | None:intergenic |
| ATCAGCATGAGATTCAGCTA+TGG | 0.626225 | 7:+10185789 | None:intergenic |
| TCTGACTGTAGAAATGGTGA+TGG | 0.632030 | 7:-10184818 | MsG0780036591.01.T01:CDS |
| AAGAAATGTGAGGTTTGGTG+TGG | 0.632754 | 7:+10185952 | None:intergenic |
| TAGAAAGGACAAAATTCATG+TGG | 0.641435 | 7:+10185505 | None:intergenic |
| AATTCATGTGGCAAACAAAG+GGG | 0.665834 | 7:+10185517 | None:intergenic |
| AAGGGTATTGAAGAAATGTG+AGG | 0.671198 | 7:+10185942 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACATATTTTGGAAATTGTAT+TGG | - | Chr7:10185708-10185727 | MsG0780036591.01.T01:CDS | 20.0% |
| !!! | ATTACTTTTCTATCATAGAA+AGG | + | Chr7:10185345-10185364 | None:intergenic | 20.0% |
| !!! | TTTGAAACAAGTGAATTATT+TGG | - | Chr7:10185747-10185766 | MsG0780036591.01.T01:CDS | 20.0% |
| ! | AAAACCAAAGAAAGTTGAAA+TGG | - | Chr7:10185959-10185978 | MsG0780036591.01.T01:CDS | 25.0% |
| ! | AAATCCATAGTTTGGAAAAA+CGG | + | Chr7:10185178-10185197 | None:intergenic | 25.0% |
| ! | AGTGAATTATTTGGAGAAAA+TGG | - | Chr7:10185756-10185775 | MsG0780036591.01.T01:CDS | 25.0% |
| ! | ATTTGTAGTGTCAAAACAAA+AGG | - | Chr7:10185524-10185543 | MsG0780036591.01.T01:CDS | 25.0% |
| ! | CTATGATAGAAAAGTAATCA+AGG | - | Chr7:10185347-10185366 | MsG0780036591.01.T01:CDS | 25.0% |
| ! | GTACTATAAACCTCAAATTT+AGG | + | Chr7:10185924-10185943 | None:intergenic | 25.0% |
| ! | TAACTTTCTTTGACATACTA+TGG | - | Chr7:10184815-10184834 | MsG0780036591.01.T01:CDS | 25.0% |
| ! | TAGAAGAAAATTCTTTGAAC+AGG | + | Chr7:10184853-10184872 | None:intergenic | 25.0% |
| ! | TTCCAAATGATTCATTTAGA+GGG | - | Chr7:10185460-10185479 | MsG0780036591.01.T01:CDS | 25.0% |
| ! | TTTGTAGTGTCAAAACAAAA+GGG | - | Chr7:10185525-10185544 | MsG0780036591.01.T01:CDS | 25.0% |
| !! | GACTTCTTTTATCAAATCTT+GGG | - | Chr7:10185245-10185264 | MsG0780036591.01.T01:CDS | 25.0% |
| !! | GTTATTTCAATTATCTCGTT+TGG | - | Chr7:10185482-10185501 | MsG0780036591.01.T01:CDS | 25.0% |
| !! | TATTTTGCCTCTAATTCATT+TGG | + | Chr7:10185375-10185394 | None:intergenic | 25.0% |
| !! | TCATGTGAAATATTCAAGTT+AGG | + | Chr7:10185126-10185145 | None:intergenic | 25.0% |
| !!! | TATTTTGGAAATTGTATTGG+AGG | - | Chr7:10185711-10185730 | MsG0780036591.01.T01:CDS | 25.0% |
| AAAATTCATGTGGCAAACAA+AGG | + | Chr7:10185320-10185339 | None:intergenic | 30.0% | |
| AAATTCATGTGGCAAACAAA+GGG | + | Chr7:10185319-10185338 | None:intergenic | 30.0% | |
| AACCAACAATAGAAGCTTAA+TGG | - | Chr7:10185422-10185441 | MsG0780036591.01.T01:CDS | 30.0% | |
| AACCATTAAGCTTCTATTGT+TGG | + | Chr7:10185427-10185446 | None:intergenic | 30.0% | |
| AACCCTCTAAATGAATCATT+TGG | + | Chr7:10185465-10185484 | None:intergenic | 30.0% | |
| AAGAAAATTCTTTGAACAGG+TGG | + | Chr7:10184850-10184869 | None:intergenic | 30.0% | |
| AATTGCTTTAACATCGGTAA+TGG | + | Chr7:10185886-10185905 | None:intergenic | 30.0% | |
| ACAATAGAAGCTTAATGGTT+TGG | - | Chr7:10185427-10185446 | MsG0780036591.01.T01:CDS | 30.0% | |
| ACCAATCATCAAATTCATCA+AGG | - | Chr7:10185002-10185021 | MsG0780036591.01.T01:CDS | 30.0% | |
| AGGCAAAATATTTGAGTGAT+TGG | - | Chr7:10185385-10185404 | MsG0780036591.01.T01:CDS | 30.0% | |
| AGTATGTCAAAGAAAGTTAG+AGG | + | Chr7:10184814-10184833 | None:intergenic | 30.0% | |
| ATGGTTTCTATAGATAGAAC+TGG | - | Chr7:10185978-10185997 | MsG0780036591.01.T01:CDS | 30.0% | |
| ATTACCGATGTTAAAGCAAT+TGG | - | Chr7:10185885-10185904 | MsG0780036591.01.T01:CDS | 30.0% | |
| CAATAGAAGCTTAATGGTTT+GGG | - | Chr7:10185428-10185447 | MsG0780036591.01.T01:CDS | 30.0% | |
| CAATTTCCAAAATATGTCGT+AGG | + | Chr7:10185705-10185724 | None:intergenic | 30.0% | |
| CCAATCATCAAATTCATCAA+GGG | - | Chr7:10185003-10185022 | MsG0780036591.01.T01:CDS | 30.0% | |
| CCAATTGAAAATCCATAGTT+TGG | + | Chr7:10185186-10185205 | None:intergenic | 30.0% | |
| GAAACCATTTCAACTTTCTT+TGG | + | Chr7:10185966-10185985 | None:intergenic | 30.0% | |
| GTTCCAAATGATTCATTTAG+AGG | - | Chr7:10185459-10185478 | MsG0780036591.01.T01:CDS | 30.0% | |
| TAGAAAGGACAAAATTCATG+TGG | + | Chr7:10185330-10185349 | None:intergenic | 30.0% | |
| TATTGAAGAAATGTGAGGTT+TGG | + | Chr7:10184888-10184907 | None:intergenic | 30.0% | |
| TGAGAACAAAAATGTTGTGA+TGG | - | Chr7:10185638-10185657 | MsG0780036591.01.T01:CDS | 30.0% | |
| TGTGTCTAACAAAAAAGCAA+TGG | - | Chr7:10186052-10186071 | MsG0780036591.01.T01:CDS | 30.0% | |
| ! | ATAAAAGTGAGAAAGTGCAA+TGG | + | Chr7:10184941-10184960 | None:intergenic | 30.0% |
| ! | CCAAACTATGGATTTTCAAT+TGG | - | Chr7:10185183-10185202 | MsG0780036591.01.T01:CDS | 30.0% |
| ! | CGACTTCTTTTATCAAATCT+TGG | - | Chr7:10185244-10185263 | MsG0780036591.01.T01:CDS | 30.0% |
| ! | GAAAGTGATTGTTTGAGTTT+GGG | + | Chr7:10184919-10184938 | None:intergenic | 30.0% |
| ! | GCTGATTTCAATCATTTATC+TGG | - | Chr7:10185060-10185079 | MsG0780036591.01.T01:CDS | 30.0% |
| !! | ATAAAGAATGGAGTGTTGAA+TGG | - | Chr7:10185822-10185841 | MsG0780036591.01.T01:CDS | 30.0% |
| !! | ATTGGTTTGTGAGAATCATT+AGG | + | Chr7:10184988-10185007 | None:intergenic | 30.0% |
| !! | GCTTTGGAAACTATAAAGAA+TGG | - | Chr7:10185810-10185829 | MsG0780036591.01.T01:CDS | 30.0% |
| !! | TCTCACTTTTATCCTCTTTT+AGG | - | Chr7:10184949-10184968 | MsG0780036591.01.T01:CDS | 30.0% |
| !!! | GAGGTTTATAGTACTGATTT+TGG | - | Chr7:10185930-10185949 | MsG0780036591.01.T01:CDS | 30.0% |
| !!! | TATAGTACTGATTTTGGTTG+TGG | - | Chr7:10185936-10185955 | MsG0780036591.01.T01:CDS | 30.0% |
| AAAGAAAGTTAGAGGAAGTG+AGG | + | Chr7:10184806-10184825 | None:intergenic | 35.0% | |
| AATTCATGTGGCAAACAAAG+GGG | + | Chr7:10185318-10185337 | None:intergenic | 35.0% | |
| AGCACCAATTGCTTTAACAT+CGG | + | Chr7:10185892-10185911 | None:intergenic | 35.0% | |
| CAAATGAGATGACCTAAAAG+AGG | + | Chr7:10184964-10184983 | None:intergenic | 35.0% | |
| CAAGGATCCAAATGAATTAG+AGG | - | Chr7:10185365-10185384 | MsG0780036591.01.T01:CDS | 35.0% | |
| CCAAAATATGTCGTAGGAAT+CGG | + | Chr7:10185699-10185718 | None:intergenic | 35.0% | |
| GGTATCTATTGCTTATGCAT+TGG | - | Chr7:10185590-10185609 | MsG0780036591.01.T01:CDS | 35.0% | |
| TCTACAGTCAGAAAGACAAA+AGG | + | Chr7:10186006-10186025 | None:intergenic | 35.0% | |
| TCTTCACTTGTCAACATTTG+TGG | - | Chr7:10185569-10185588 | MsG0780036591.01.T01:CDS | 35.0% | |
| TGTCTTTCTGACTGTAGAAA+TGG | - | Chr7:10186008-10186027 | MsG0780036591.01.T01:CDS | 35.0% | |
| TGTTGAATGGAGCTGAAAAT+TGG | - | Chr7:10185835-10185854 | MsG0780036591.01.T01:CDS | 35.0% | |
| TTTGGAGATCGAAAAGCTTA+AGG | - | Chr7:10185500-10185519 | MsG0780036591.01.T01:CDS | 35.0% | |
| ! | ACATGATCAAGCTTCTGTTT+TGG | - | Chr7:10185140-10185159 | MsG0780036591.01.T01:CDS | 35.0% |
| ! | ATTGTTTGAGTTTGGGAAGA+AGG | + | Chr7:10184912-10184931 | None:intergenic | 35.0% |
| ! | CCCTTGATGAATTTGATGAT+TGG | + | Chr7:10185006-10185025 | None:intergenic | 35.0% |
| ! | GGAAAGTGATTGTTTGAGTT+TGG | + | Chr7:10184920-10184939 | None:intergenic | 35.0% |
| ! | TTGAGTGATTGGTTAAAGCA+AGG | - | Chr7:10185396-10185415 | MsG0780036591.01.T01:CDS | 35.0% |
| ! | TTGTTTGAGTTTGGGAAGAA+GGG | + | Chr7:10184911-10184930 | None:intergenic | 35.0% |
| !! | AAGGGTATTGAAGAAATGTG+AGG | + | Chr7:10184893-10184912 | None:intergenic | 35.0% |
| !! | CTCTTTTAGGTCATCTCATT+TGG | - | Chr7:10184962-10184981 | MsG0780036591.01.T01:CDS | 35.0% |
| !! | GTAACCGTTTTTCCAAACTA+TGG | - | Chr7:10185171-10185190 | MsG0780036591.01.T01:CDS | 35.0% |
| !! | TGGAGAAAATGGACTAATTG+TGG | - | Chr7:10185767-10185786 | MsG0780036591.01.T01:CDS | 35.0% |
| !! | TGTCAAAACAAAAGGGTACT+AGG | - | Chr7:10185532-10185551 | MsG0780036591.01.T01:CDS | 35.0% |
| !!! | TCACATCATGCTGTTTTAGA+TGG | - | Chr7:10185213-10185232 | MsG0780036591.01.T01:CDS | 35.0% |
| ATCAGCATGAGATTCAGCTA+TGG | + | Chr7:10185046-10185065 | None:intergenic | 40.0% | |
| GGTAGTTCTTCTTGTGAAGA+TGG | + | Chr7:10184775-10184794 | None:intergenic | 40.0% | |
| TCTGACTGTAGAAATGGTGA+TGG | - | Chr7:10186014-10186033 | MsG0780036591.01.T01:CDS | 40.0% | |
| TGGTCTTCATGGTTACTTGA+AGG | - | Chr7:10185855-10185874 | MsG0780036591.01.T01:CDS | 40.0% | |
| TTTGCTTCGCTCTTTGTCAA+AGG | - | Chr7:10186080-10186099 | MsG0780036591.01.T01:CDS | 40.0% | |
| ! | AGTGATTGGTTAAAGCAAGG+TGG | - | Chr7:10185399-10185418 | MsG0780036591.01.T01:CDS | 40.0% |
| ! | CCGATTCCTACGACATATTT+TGG | - | Chr7:10185696-10185715 | MsG0780036591.01.T01:CDS | 40.0% |
| !! | AAGAAATGTGAGGTTTGGTG+TGG | + | Chr7:10184883-10184902 | None:intergenic | 40.0% |
| !! | AATGGTGATGGTGTTGAGAT+AGG | - | Chr7:10186026-10186045 | MsG0780036591.01.T01:CDS | 40.0% |
| !! | GAGCTGAAAATTGGTCTTCA+TGG | - | Chr7:10185844-10185863 | MsG0780036591.01.T01:CDS | 40.0% |
| !! | TGAAGTGTTGAGTGAAGCTT+TGG | - | Chr7:10185794-10185813 | MsG0780036591.01.T01:CDS | 40.0% |
| !! | TGCTGGTTCACCTAAATTTG+AGG | - | Chr7:10185911-10185930 | MsG0780036591.01.T01:CDS | 40.0% |
| !! | TGGCTTTGAATATCGATTGC+CGG | - | Chr7:10185658-10185677 | MsG0780036591.01.T01:CDS | 40.0% |
| AGAGGAAGTGAGGTTTCTGA+AGG | + | Chr7:10184796-10184815 | None:intergenic | 45.0% | |
| TCGGCTGATCTAAACGATTC+CGG | + | Chr7:10185680-10185699 | None:intergenic | 45.0% | |
| !! | GTTAAAGCAATTGGTGCTGC+TGG | - | Chr7:10185894-10185913 | MsG0780036591.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 10184739 | 10186115 | 10184739 | ID=MsG0780036591.01;Name=MsG0780036591.01 |
| Chr7 | mRNA | 10184739 | 10186115 | 10184739 | ID=MsG0780036591.01.T01;Parent=MsG0780036591.01;Name=MsG0780036591.01.T01;_AED=0.12;_eAED=0.12;_QI=0|-1|0|1|-1|1|1|0|458 |
| Chr7 | exon | 10184739 | 10186115 | 10184739 | ID=MsG0780036591.01.T01:exon:10414;Parent=MsG0780036591.01.T01 |
| Chr7 | CDS | 10184739 | 10186115 | 10184739 | ID=MsG0780036591.01.T01:cds;Parent=MsG0780036591.01.T01 |
| Gene Sequence |
| Protein sequence |
