Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039252.01.T01 | KEH23190.1 | 78.511 | 591 | 10 | 4 | 1 | 474 | 1 | 591 | 0 | 909 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039252.01.T01 | A0A072UBG4 | 78.511 | 591 | 10 | 4 | 1 | 474 | 1 | 591 | 0.0 | 909 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047797.01 | MsG0780039252.01 | 0.825894 | 3.654093e-54 | 3.374392e-51 |
| MsG0080047869.01 | MsG0780039252.01 | 0.856307 | 3.515719e-62 | 8.353836e-59 |
| MsG0080047944.01 | MsG0780039252.01 | 0.809061 | 2.279579e-50 | 1.324979e-47 |
| MsG0080047957.01 | MsG0780039252.01 | 0.812671 | 3.770630e-51 | 2.412171e-48 |
| MsG0080047959.01 | MsG0780039252.01 | 0.819172 | 1.335310e-52 | 1.019964e-49 |
| MsG0080048058.01 | MsG0780039252.01 | 0.803769 | 2.976135e-49 | 1.508353e-46 |
| MsG0080048402.01 | MsG0780039252.01 | 0.831908 | 1.278293e-55 | 1.406810e-52 |
| MsG0080048461.01 | MsG0780039252.01 | 0.867441 | 1.365661e-65 | 4.768874e-62 |
| MsG0080048462.01 | MsG0780039252.01 | 0.848119 | 7.509064e-60 | 1.363680e-56 |
| MsG0080048618.01 | MsG0780039252.01 | 0.822006 | 2.983705e-53 | 2.466689e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039252.01.T01 | MTR_7g068890 | 78.511 | 591 | 10 | 4 | 1 | 474 | 1 | 591 | 0.0 | 909 |
| MsG0780039252.01.T01 | MTR_5g023960 | 38.014 | 584 | 233 | 10 | 14 | 470 | 10 | 591 | 1.84e-127 | 383 |
| MsG0780039252.01.T01 | MTR_5g023960 | 38.356 | 584 | 230 | 11 | 14 | 470 | 10 | 590 | 1.30e-126 | 380 |
| MsG0780039252.01.T01 | MTR_4g032925 | 24.012 | 329 | 173 | 7 | 51 | 305 | 100 | 425 | 1.25e-17 | 86.3 |
| MsG0780039252.01.T01 | MTR_4g032925 | 24.012 | 329 | 173 | 7 | 51 | 305 | 100 | 425 | 1.90e-17 | 85.9 |
| MsG0780039252.01.T01 | MTR_4g032935 | 30.811 | 185 | 97 | 6 | 149 | 305 | 19 | 200 | 8.07e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039252.01.T01 | AT3G51830 | 52.961 | 591 | 158 | 6 | 1 | 474 | 1 | 588 | 0.0 | 607 |
| MsG0780039252.01.T01 | AT3G51460 | 38.151 | 595 | 233 | 13 | 6 | 470 | 3 | 592 | 1.25e-124 | 375 |
| MsG0780039252.01.T01 | AT5G66020 | 36.318 | 592 | 237 | 12 | 12 | 470 | 4 | 588 | 3.08e-118 | 359 |
| MsG0780039252.01.T01 | AT3G51830 | 50.980 | 204 | 77 | 3 | 1 | 204 | 1 | 181 | 1.20e-61 | 201 |
| MsG0780039252.01.T01 | AT3G14205 | 25.751 | 466 | 218 | 13 | 65 | 404 | 91 | 554 | 1.97e-27 | 116 |
| MsG0780039252.01.T01 | AT3G43220 | 23.276 | 464 | 226 | 13 | 68 | 404 | 89 | 549 | 2.80e-23 | 103 |
| MsG0780039252.01.T01 | AT3G43220 | 23.276 | 464 | 226 | 13 | 68 | 404 | 89 | 549 | 2.81e-23 | 103 |
| MsG0780039252.01.T01 | AT3G14205 | 27.711 | 332 | 158 | 11 | 153 | 404 | 50 | 379 | 5.54e-20 | 93.6 |
| MsG0780039252.01.T01 | AT3G43220 | 22.944 | 462 | 230 | 14 | 68 | 404 | 89 | 549 | 2.27e-19 | 91.7 |
| MsG0780039252.01.T01 | AT1G22620 | 23.718 | 312 | 167 | 8 | 65 | 305 | 106 | 417 | 5.18e-15 | 78.2 |
Find 93 sgRNAs with CRISPR-Local
Find 343 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGTGGAGTCAATGCC+GGG | 0.491746 | 7:-60794578 | MsG0780039252.01.T01:CDS |
| AAATGACGCTCCACAACATT+TGG | 0.393961 | 7:+60793021 | None:intergenic |
| AAGGAAGTTGGAAGTTTCCT+TGG | 0.464170 | 7:-60794804 | MsG0780039252.01.T01:CDS |
| AAGGCTCAAAGCTACTCCGC+TGG | 0.559278 | 7:+60790493 | None:intergenic |
| AAGTTGAAAACTTCAAAGCC+TGG | 0.516357 | 7:+60794736 | None:intergenic |
| AATCGTCAATTGCAGAGGGT+TGG | 0.525773 | 7:-60791132 | MsG0780039252.01.T01:CDS |
| AATCTCCTAATGAAGGGTTT+TGG | 0.295846 | 7:-60795760 | MsG0780039252.01.T01:CDS |
| AATTAATGTTTCCATCAACA+CGG | 0.649289 | 7:+60795732 | None:intergenic |
| ACAAAGATATGGAGAGGTAT+TGG | 0.455710 | 7:-60792986 | MsG0780039252.01.T01:CDS |
| ACAATCTGCTCCCACAAAAT+AGG | 0.284131 | 7:+60793200 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATAACATGTCCCACAT+CGG | - | Chr7:60793612-60793631 | MsG0780039252.01.T01:CDS | 30.0% | |
| AAATTCATCAGTATCACAGA+AGG | + | Chr7:60795171-60795190 | None:intergenic | 30.0% | |
| AACTTGCATTATACTCGTTA+AGG | - | Chr7:60791140-60791159 | MsG0780039252.01.T01:CDS | 30.0% | |
| AAGCTGCCATAAAAAAAGTT+TGG | + | Chr7:60792178-60792197 | None:intergenic | 30.0% | |
| AATCATATATCCTATAGGCA+GGG | + | Chr7:60794150-60794169 | None:intergenic | 30.0% | |
| AATGACAGTTAATTGAAAGG+TGG | - | Chr7:60795420-60795439 | MsG0780039252.01.T01:intron | 30.0% | |
| ACTCTAATTCATTCCTTTGT+AGG | - | Chr7:60792783-60792802 | MsG0780039252.01.T01:intron | 30.0% | |
| AGAAGAAGACTTTCCTTAAT+TGG | + | Chr7:60791007-60791026 | None:intergenic | 30.0% | |
| AGATGCATGCTATATATCTT+TGG | + | Chr7:60793155-60793174 | None:intergenic | 30.0% | |
| AGTAACATTTGTTCGATCAA+GGG | + | Chr7:60794760-60794779 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 60790054 | 60795988 | 60790054 | ID=MsG0780039252.01;Name=MsG0780039252.01 |
| Chr7 | mRNA | 60790054 | 60795988 | 60790054 | ID=MsG0780039252.01.T01;Parent=MsG0780039252.01;Name=MsG0780039252.01.T01;_AED=0.38;_eAED=0.39;_QI=106|0.71|0.73|1|1|1|15|0|474 |
| Chr7 | exon | 60790054 | 60790164 | 60790054 | ID=MsG0780039252.01.T01:exon:7407;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60790488 | 60790574 | 60790488 | ID=MsG0780039252.01.T01:exon:7406;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60790921 | 60790988 | 60790921 | ID=MsG0780039252.01.T01:exon:7405;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60791070 | 60791178 | 60791070 | ID=MsG0780039252.01.T01:exon:7404;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60791260 | 60791359 | 60791260 | ID=MsG0780039252.01.T01:exon:7403;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60791473 | 60791571 | 60791473 | ID=MsG0780039252.01.T01:exon:7402;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60791697 | 60791761 | 60791697 | ID=MsG0780039252.01.T01:exon:7401;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60792966 | 60793043 | 60792966 | ID=MsG0780039252.01.T01:exon:7400;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60793172 | 60793237 | 60793172 | ID=MsG0780039252.01.T01:exon:7399;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60793395 | 60793501 | 60793395 | ID=MsG0780039252.01.T01:exon:7398;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60793587 | 60793666 | 60793587 | ID=MsG0780039252.01.T01:exon:7397;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60794542 | 60794633 | 60794542 | ID=MsG0780039252.01.T01:exon:7396;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60794728 | 60794855 | 60794728 | ID=MsG0780039252.01.T01:exon:7395;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60795162 | 60795236 | 60795162 | ID=MsG0780039252.01.T01:exon:7394;Parent=MsG0780039252.01.T01 |
| Chr7 | exon | 60795723 | 60795988 | 60795723 | ID=MsG0780039252.01.T01:exon:7393;Parent=MsG0780039252.01.T01 |
| Chr7 | five_prime_UTR | 60795883 | 60795988 | 60795883 | ID=MsG0780039252.01.T01:five_prime_utr;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60795723 | 60795882 | 60795723 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60795162 | 60795236 | 60795162 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60794728 | 60794855 | 60794728 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60794542 | 60794633 | 60794542 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60793587 | 60793666 | 60793587 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60793395 | 60793501 | 60793395 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60793172 | 60793237 | 60793172 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60792966 | 60793043 | 60792966 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60791697 | 60791761 | 60791697 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60791473 | 60791571 | 60791473 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60791260 | 60791359 | 60791260 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60791070 | 60791178 | 60791070 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60790921 | 60790988 | 60790921 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60790488 | 60790574 | 60790488 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Chr7 | CDS | 60790054 | 60790164 | 60790054 | ID=MsG0780039252.01.T01:cds;Parent=MsG0780039252.01.T01 |
| Gene Sequence |
>MsG0780039252.01.T01 ATGGACATTGAACCCACTTCAGATTCTTCTGAAAGATTCAAGCTTTATGATGAGCTTGAATTGCAAGAATTTCAAGACAAATTTGTGATCAAATCTCATCAATCTCCTAATGAAGGGTTTTGGATTAGCCGTGTTGATGGAAACATTAATTTACTTGATGGTGACGCAGTCTCAGAAAGTCCGACGAAGACATCTACGATATATGGTGTCGTGGGAACAATAAGATTGGTCGTAGGAACTTATGCCATTGTAATAACTTCTCGGAAGGAAGTTGGAAGTTTCCTTGGTTTTCCGGTATATCGCTTGATGTCGATGAGAGTACTTGCGTGTAACCAGGCTTTGAAGTTTTCAACTTCTCAAGAAAAAAAGGATGAGGCGTACTTTTTGACATTGTTGAAAATAGTGGAGTCAATGCCGGGTTTATACTATTCTTATGAAACCGATATTACATTAAACTTCCAAACAGCTGAGCTGAAGCTAAAAGGCTCATGTGCTAAACTGTCATTAATTTCAAGGAGATGTACACGGCGTTTAGGAACAAGAATGTGGAGAAGAGGAGCTAACTTTGAAGGTGACTCTGCCAATTTCATTGAAACTGAACAGTTGCTTGAAGTTGAAGAATTCAAGTTCTCATTTTTGCAGGTTCGAGGTTCGATTCCTATTTTGTGGGAGCAGATTGTTGATCTCAGCTATAAGCCTCACCTTCGGCCAAATGTTGTGGAGCGTCATTTCCATGATCTCTCACAAAGATATGGAGAGGTATTGGCAGTTGATCTAACTGACAAACATGGTGAAGAAGGTAAATTAAGTGCAGCATATGCTGCTGAAATGAAAAATCAGCAAAGTGCGAGATATGTGCCATTTGATTTCCATTGCCACTGTCGGGGCTCAAATTTTGATGATTTGAAAATCCTCTACGATCAAATTTCAGACGATTTTGAAAAACAAAGATACTTCATGATAGATAGAAAAGGCGATATACTAGAGGAGCAGAAAGGAATTATCAGATCAAACTGCATTGATTCCCTTGATCGAACAAATGTTACTCAGTGTTATATGGCCCAGAAAGCATTAAATCGTCAATTGCAGAGGGTTGGAGTGCTTACTTGCTCTGAGTGCATTTCAATGTTCGACGAGGAATATGGAAAATTTAGAATATTGTGGGCAGAGCAAGGTGATGAGATAAGCCTTGAGTATGCTGGGACTAATGCCCTCAAAGGAGACTTAGATGCCTTGGATCTGATAAGCGGTCACTATACTGTCAGCAGAAACATTCCTTCTCACTTCCAGCGGAGTAGCTTTGAGCCTTTAACTCAGTATGTGTCTTCTGTACTGTGTGCTGGAATAACTGCTGGAGTCATGGCAATTGTCAAAGCTAATGGAAGGCAGTTCTGTTCAAGGCCTCGTTTGTGCGGTCTCATGTGA |
| Protein sequence |
>MsG0780039252.01.T01 MDIEPTSDSSERFKLYDELELQEFQDKFVIKSHQSPNEGFWISRVDGNINLLDGDAVSESPTKTSTIYGVVGTIRLVVGTYAIVITSRKEVGSFLGFPVYRLMSMRVLACNQALKFSTSQEKKDEAYFLTLLKIVESMPGLYYSYETDITLNFQTAELKLKGSCAKLSLISRRCTRRLGTRMWRRGANFEGDSANFIETEQLLEVEEFKFSFLQVRGSIPILWEQIVDLSYKPHLRPNVVERHFHDLSQRYGEVLAVDLTDKHGEEGKLSAAYAAEMKNQQSARYVPFDFHCHCRGSNFDDLKILYDQISDDFEKQRYFMIDRKGDILEEQKGIIRSNCIDSLDRTNVTQCYMAQKALNRQLQRVGVLTCSECISMFDEEYGKFRILWAEQGDEISLEYAGTNALKGDLDALDLISGHYTVSRNIPSHFQRSSFEPLTQYVSSVLCAGITAGVMAIVKANGRQFCSRPRLCGLM* |
