Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036722.01.T01 | AFK47155.1 | 98.039 | 102 | 2 | 0 | 4 | 105 | 47 | 148 | 3.56E-67 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036722.01.T01 | P60040 | 87.255 | 102 | 13 | 0 | 4 | 105 | 141 | 242 | 1.66E-63 | 195 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036722.01.T01 | I3T3R3 | 98.039 | 102 | 2 | 0 | 4 | 105 | 47 | 148 | 1.70e-67 | 208 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002377.01 | MsG0780036722.01 | 0.850678 | 1.453929e-60 | 2.870363e-57 |
| MsG0180004264.01 | MsG0780036722.01 | 0.819191 | 1.321917e-52 | 1.010276e-49 |
| MsG0180004389.01 | MsG0780036722.01 | 0.812552 | 4.002699e-51 | 2.552719e-48 |
| MsG0280006324.01 | MsG0780036722.01 | 0.806018 | 1.008428e-49 | 5.415295e-47 |
| MsG0380013475.01 | MsG0780036722.01 | 0.801961 | 7.034223e-49 | 3.403922e-46 |
| MsG0380014537.01 | MsG0780036722.01 | 0.804380 | 2.220666e-49 | 1.143302e-46 |
| MsG0680030880.01 | MsG0780036722.01 | 0.809257 | 2.069337e-50 | 1.209125e-47 |
| MsG0680030882.01 | MsG0780036722.01 | 0.814193 | 1.745424e-51 | 1.163388e-48 |
| MsG0480018465.01 | MsG0780036722.01 | 0.804849 | 1.773441e-49 | 9.241961e-47 |
| MsG0480021870.01 | MsG0780036722.01 | 0.800133 | 1.662631e-48 | 7.682061e-46 |
| MsG0480022068.01 | MsG0780036722.01 | 0.802706 | 4.940409e-49 | 2.436662e-46 |
| MsG0480023463.01 | MsG0780036722.01 | 0.812075 | 5.088895e-51 | 3.203980e-48 |
| MsG0580024690.01 | MsG0780036722.01 | 0.813579 | 2.382752e-51 | 1.562043e-48 |
| MsG0380015332.01 | MsG0780036722.01 | 0.826510 | 2.608384e-54 | 2.451178e-51 |
| MsG0380015555.01 | MsG0780036722.01 | 0.800645 | 1.307736e-48 | 6.121274e-46 |
| MsG0380016529.01 | MsG0780036722.01 | 0.801027 | 1.092634e-48 | 5.164638e-46 |
| MsG0380017589.01 | MsG0780036722.01 | 0.812221 | 4.728037e-51 | 2.988793e-48 |
| MsG0380018075.01 | MsG0780036722.01 | 0.801210 | 1.002378e-48 | 4.759670e-46 |
| MsG0480018183.01 | MsG0780036722.01 | 0.804934 | 1.701577e-49 | 8.887095e-47 |
| MsG0280007955.01 | MsG0780036722.01 | 0.804544 | 2.052804e-49 | 1.061301e-46 |
| MsG0280010350.01 | MsG0780036722.01 | 0.801398 | 9.174268e-49 | 4.376737e-46 |
| MsG0280010366.01 | MsG0780036722.01 | 0.826770 | 2.261036e-54 | 2.140515e-51 |
| MsG0680035692.01 | MsG0780036722.01 | 0.807159 | 5.791134e-50 | 3.202227e-47 |
| MsG0780036602.01 | MsG0780036722.01 | 0.807566 | 4.747497e-50 | 2.652990e-47 |
| MsG0780036722.01 | MsG0780037054.01 | 0.817325 | 3.497214e-52 | 2.538917e-49 |
| MsG0780036722.01 | MsG0780037292.01 | 0.801888 | 7.281399e-49 | 3.517317e-46 |
| MsG0780036722.01 | MsG0780038377.01 | 0.801109 | 1.051644e-48 | 4.980935e-46 |
| MsG0780036722.01 | MsG0780039443.01 | 0.825427 | 4.714417e-54 | 4.295568e-51 |
| MsG0780036722.01 | MsG0880042520.01 | 0.806354 | 8.569260e-50 | 4.641088e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036722.01.T01 | MTR_2g038250 | 98.039 | 102 | 2 | 0 | 4 | 105 | 143 | 244 | 8.88e-71 | 211 |
| MsG0780036722.01.T01 | MTR_1g022385 | 98.039 | 102 | 2 | 0 | 4 | 105 | 174 | 275 | 1.56e-70 | 211 |
| MsG0780036722.01.T01 | MTR_4g008160 | 89.216 | 102 | 11 | 0 | 4 | 105 | 142 | 243 | 4.07e-65 | 197 |
| MsG0780036722.01.T01 | MTR_1g022375 | 78.750 | 80 | 17 | 0 | 16 | 95 | 77 | 156 | 1.45e-42 | 137 |
| MsG0780036722.01.T01 | MTR_7g059170 | 54.902 | 102 | 46 | 0 | 4 | 105 | 145 | 246 | 5.73e-41 | 135 |
| MsG0780036722.01.T01 | MTR_1g108785 | 53.448 | 58 | 21 | 4 | 46 | 97 | 2 | 59 | 5.92e-12 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036722.01.T01 | AT2G01250 | 87.255 | 102 | 13 | 0 | 4 | 105 | 141 | 242 | 1.69e-64 | 195 |
| MsG0780036722.01.T01 | AT2G44120 | 85.294 | 102 | 15 | 0 | 4 | 105 | 141 | 242 | 6.06e-64 | 194 |
| MsG0780036722.01.T01 | AT2G44120 | 85.294 | 102 | 15 | 0 | 4 | 105 | 146 | 247 | 8.05e-64 | 194 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT3G13580 | 84.314 | 102 | 16 | 0 | 4 | 105 | 143 | 244 | 8.47e-63 | 191 |
| MsG0780036722.01.T01 | AT1G80750 | 50.980 | 102 | 49 | 1 | 5 | 105 | 146 | 247 | 4.52e-29 | 105 |
| MsG0780036722.01.T01 | AT2G01250 | 72.973 | 37 | 10 | 0 | 4 | 40 | 141 | 177 | 2.76e-11 | 57.4 |
Find 34 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCATTAGAAGGATGAATT+AGG | 0.270598 | 7:+12481997 | MsG0780036722.01.T01:CDS |
| GTGTCGTTTAAGATTGATTA+TGG | 0.274625 | 7:+12482029 | MsG0780036722.01.T01:three_prime_UTR |
| TAGAAGGATGAATTAGGTTT+TGG | 0.280297 | 7:+12482003 | MsG0780036722.01.T01:exon |
| TTTCAAGCTCAAAGCTCCTT+TGG | 0.284641 | 7:+12481901 | MsG0780036722.01.T01:CDS |
| AGTGTTCAATTGCAGGTTCT+TGG | 0.317764 | 7:+12481788 | MsG0780036722.01.T01:intron |
| TTCAAGCTCAAAGCTCCTTT+GGG | 0.329811 | 7:+12481902 | MsG0780036722.01.T01:CDS |
| TTGATCTACAAGCGCGGTTA+TGG | 0.337108 | 7:+12481439 | MsG0780036722.01.T01:CDS |
| TCAATTCTCTAACACTCTTC+AGG | 0.373375 | 7:-12481420 | None:intergenic |
| AAAACTTTCACTGCACTTGC+AGG | 0.373710 | 7:-12482150 | None:intergenic |
| GTGTTCAATTGCAGGTTCTT+GGG | 0.403327 | 7:+12481789 | MsG0780036722.01.T01:intron |
| TTCAGGATCAACATAATTAA+CGG | 0.449774 | 7:-12481403 | None:intergenic |
| ACATCAATGAACTCATTAGA+AGG | 0.462677 | 7:+12481987 | MsG0780036722.01.T01:CDS |
| TGAAGTGAGGTCCAACAGTC+AGG | 0.464367 | 7:-12481853 | None:intergenic |
| AGATAACTCTATCATTGAAC+AGG | 0.482014 | 7:+12481492 | MsG0780036722.01.T01:CDS |
| AAGAGAAATCACTATGTTGA+AGG | 0.489111 | 7:+12481938 | MsG0780036722.01.T01:CDS |
| AAAGGAGCTTTGAGCTTGAA+AGG | 0.504831 | 7:-12481899 | None:intergenic |
| GAGAGGCAAACAACTTCCTA+TGG | 0.504947 | 7:+12481876 | MsG0780036722.01.T01:CDS |
| TATGTTGAAGGTGGTGATGC+TGG | 0.533299 | 7:+12481950 | MsG0780036722.01.T01:CDS |
| TAGAATCTGAAGTCAAATGA+AGG | 0.542280 | 7:-12481322 | None:intergenic |
| TTGCAGGTTCTTGGGAAACA+TGG | 0.547799 | 7:+12481797 | MsG0780036722.01.T01:intron |
| GCGGTTATGGAAAGGTTGAT+AGG | 0.549876 | 7:+12481452 | MsG0780036722.01.T01:CDS |
| TTCTTCTTCATGCCACCCAA+AGG | 0.554983 | 7:-12481917 | None:intergenic |
| ATCCACGAGATCCTGACTGT+TGG | 0.556683 | 7:+12481842 | MsG0780036722.01.T01:CDS |
| AGGTGGTGATGCTGGTAAGA+GGG | 0.564495 | 7:+12481958 | MsG0780036722.01.T01:CDS |
| CTTTGAGCTTGAAAGGCCAT+AGG | 0.568033 | 7:-12481892 | None:intergenic |
| AAGCTCAAAGCTCCTTTGGG+TGG | 0.570830 | 7:+12481905 | MsG0780036722.01.T01:CDS |
| TTGTTTGCCTCTCTGAAGTG+AGG | 0.573961 | 7:-12481866 | None:intergenic |
| GTCCAACAGTCAGGATCTCG+TGG | 0.575620 | 7:-12481844 | None:intergenic |
| AAGGTGGTGATGCTGGTAAG+AGG | 0.583947 | 7:+12481957 | MsG0780036722.01.T01:CDS |
| CTACAAGCGCGGTTATGGAA+AGG | 0.599869 | 7:+12481444 | MsG0780036722.01.T01:CDS |
| TGGTGATGCTGGTAAGAGGG+AGG | 0.608332 | 7:+12481961 | MsG0780036722.01.T01:CDS |
| AGAAATCACTATGTTGAAGG+TGG | 0.637998 | 7:+12481941 | MsG0780036722.01.T01:CDS |
| AGAGAATTGATCTACAAGCG+CGG | 0.721136 | 7:+12481433 | MsG0780036722.01.T01:CDS |
| TGTTGGACCTCACTTCAGAG+AGG | 0.737396 | 7:+12481859 | MsG0780036722.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAATAGAAAAATTTAAC+CGG | - | Chr7:12481759-12481778 | None:intergenic | 15.0% |
| !!! | GAAATCTTTAAAAACAAAAA+TGG | - | Chr7:12482082-12482101 | None:intergenic | 15.0% |
| ! | TTCAGGATCAACATAATTAA+CGG | - | Chr7:12481406-12481425 | None:intergenic | 25.0% |
| !!! | GAATTTTAAAAACTACACAG+TGG | + | Chr7:12481376-12481395 | MsG0780036722.01.T01:five_prime_UTR | 25.0% |
| AAGAGAAATCACTATGTTGA+AGG | + | Chr7:12481938-12481957 | MsG0780036722.01.T01:CDS | 30.0% | |
| ACATCAATGAACTCATTAGA+AGG | + | Chr7:12481987-12482006 | MsG0780036722.01.T01:CDS | 30.0% | |
| ACTCATTAGAAGGATGAATT+AGG | + | Chr7:12481997-12482016 | MsG0780036722.01.T01:CDS | 30.0% | |
| AGATAACTCTATCATTGAAC+AGG | + | Chr7:12481492-12481511 | MsG0780036722.01.T01:CDS | 30.0% | |
| ! | TAGAAGGATGAATTAGGTTT+TGG | + | Chr7:12482003-12482022 | MsG0780036722.01.T01:exon | 30.0% |
| !! | GGTGCTATTATTATTGTTGA+AGG | + | Chr7:12481725-12481744 | MsG0780036722.01.T01:intron | 30.0% |
| !! | GTGTCGTTTAAGATTGATTA+TGG | + | Chr7:12482029-12482048 | MsG0780036722.01.T01:three_prime_UTR | 30.0% |
| AACAACTCACATTCTGACTT+TGG | - | Chr7:12481630-12481649 | None:intergenic | 35.0% | |
| AATAATAGCACCACCATACA+GGG | - | Chr7:12481717-12481736 | None:intergenic | 35.0% | |
| ACATTCTGACTTTGGCAAAT+TGG | - | Chr7:12481622-12481641 | None:intergenic | 35.0% | |
| AGAAATCACTATGTTGAAGG+TGG | + | Chr7:12481941-12481960 | MsG0780036722.01.T01:CDS | 35.0% | |
| ATTGTTGAAGGTTATGTTGC+CGG | + | Chr7:12481737-12481756 | MsG0780036722.01.T01:intron | 35.0% | |
| CATTCTGACTTTGGCAAATT+GGG | - | Chr7:12481621-12481640 | None:intergenic | 35.0% | |
| CTGAACTGTATGACATAGAT+GGG | + | Chr7:12481566-12481585 | MsG0780036722.01.T01:intron | 35.0% | |
| TAATAATAGCACCACCATAC+AGG | - | Chr7:12481718-12481737 | None:intergenic | 35.0% | |
| TCAATTCTCTAACACTCTTC+AGG | - | Chr7:12481423-12481442 | None:intergenic | 35.0% | |
| TCTGAACTGTATGACATAGA+TGG | + | Chr7:12481565-12481584 | MsG0780036722.01.T01:intron | 35.0% | |
| TGTGTGATAGAATGAAAGCA+GGG | - | Chr7:12481537-12481556 | None:intergenic | 35.0% | |
| AAAACTTTCACTGCACTTGC+AGG | - | Chr7:12482153-12482172 | None:intergenic | 40.0% | |
| AGAGAATTGATCTACAAGCG+CGG | + | Chr7:12481433-12481452 | MsG0780036722.01.T01:CDS | 40.0% | |
| AGTGTTCAATTGCAGGTTCT+TGG | + | Chr7:12481788-12481807 | MsG0780036722.01.T01:intron | 40.0% | |
| ATAATAGCACCACCATACAG+GGG | - | Chr7:12481716-12481735 | None:intergenic | 40.0% | |
| CTGACATAGTGTTCAATTGC+AGG | + | Chr7:12481781-12481800 | MsG0780036722.01.T01:intron | 40.0% | |
| CTGTGTGATAGAATGAAAGC+AGG | - | Chr7:12481538-12481557 | None:intergenic | 40.0% | |
| GTGTTCAATTGCAGGTTCTT+GGG | + | Chr7:12481789-12481808 | MsG0780036722.01.T01:intron | 40.0% | |
| TTCAAGCTCAAAGCTCCTTT+GGG | + | Chr7:12481902-12481921 | MsG0780036722.01.T01:CDS | 40.0% | |
| TTTCAAGCTCAAAGCTCCTT+TGG | + | Chr7:12481901-12481920 | MsG0780036722.01.T01:CDS | 40.0% | |
| !! | AAAGGAGCTTTGAGCTTGAA+AGG | - | Chr7:12481902-12481921 | None:intergenic | 40.0% |
| AGATGGGATCTCGCTTCATT+CGG | + | Chr7:12481582-12481601 | MsG0780036722.01.T01:intron | 45.0% | |
| CTTTGAGCTTGAAAGGCCAT+AGG | - | Chr7:12481895-12481914 | None:intergenic | 45.0% | |
| GAGAGGCAAACAACTTCCTA+TGG | + | Chr7:12481876-12481895 | MsG0780036722.01.T01:CDS | 45.0% | |
| TTCTTCTTCATGCCACCCAA+AGG | - | Chr7:12481920-12481939 | None:intergenic | 45.0% | |
| TTGATCTACAAGCGCGGTTA+TGG | + | Chr7:12481439-12481458 | MsG0780036722.01.T01:CDS | 45.0% | |
| TTGCAGGTTCTTGGGAAACA+TGG | + | Chr7:12481797-12481816 | MsG0780036722.01.T01:intron | 45.0% | |
| ! | GCGGTTATGGAAAGGTTGAT+AGG | + | Chr7:12481452-12481471 | MsG0780036722.01.T01:CDS | 45.0% |
| ! | TATGTTGAAGGTGGTGATGC+TGG | + | Chr7:12481950-12481969 | MsG0780036722.01.T01:CDS | 45.0% |
| !! | TTGTTTGCCTCTCTGAAGTG+AGG | - | Chr7:12481869-12481888 | None:intergenic | 45.0% |
| AAGCTCAAAGCTCCTTTGGG+TGG | + | Chr7:12481905-12481924 | MsG0780036722.01.T01:CDS | 50.0% | |
| ATCCACGAGATCCTGACTGT+TGG | + | Chr7:12481842-12481861 | MsG0780036722.01.T01:CDS | 50.0% | |
| CTACAAGCGCGGTTATGGAA+AGG | + | Chr7:12481444-12481463 | MsG0780036722.01.T01:CDS | 50.0% | |
| CTTGACCAATGTCCCCTGTA+TGG | + | Chr7:12481701-12481720 | MsG0780036722.01.T01:intron | 50.0% | |
| TGAAGTGAGGTCCAACAGTC+AGG | - | Chr7:12481856-12481875 | None:intergenic | 50.0% | |
| TGTTGGACCTCACTTCAGAG+AGG | + | Chr7:12481859-12481878 | MsG0780036722.01.T01:CDS | 50.0% | |
| ! | AAGGTGGTGATGCTGGTAAG+AGG | + | Chr7:12481957-12481976 | MsG0780036722.01.T01:CDS | 50.0% |
| ! | AGGTGGTGATGCTGGTAAGA+GGG | + | Chr7:12481958-12481977 | MsG0780036722.01.T01:CDS | 50.0% |
| CACCACCATACAGGGGACAT+TGG | - | Chr7:12481709-12481728 | None:intergenic | 55.0% | |
| GACCAATGTCCCCTGTATGG+TGG | + | Chr7:12481704-12481723 | MsG0780036722.01.T01:intron | 55.0% | |
| GTCCAACAGTCAGGATCTCG+TGG | - | Chr7:12481847-12481866 | None:intergenic | 55.0% | |
| ! | TGGTGATGCTGGTAAGAGGG+AGG | + | Chr7:12481961-12481980 | MsG0780036722.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 12481323 | 12482177 | 12481323 | ID=MsG0780036722.01;Name=MsG0780036722.01 |
| Chr7 | mRNA | 12481323 | 12482177 | 12481323 | ID=MsG0780036722.01.T01;Parent=MsG0780036722.01;Name=MsG0780036722.01.T01;_AED=0.13;_eAED=0.13;_QI=89|1|1|1|1|1|2|159|105 |
| Chr7 | exon | 12481323 | 12481513 | 12481323 | ID=MsG0780036722.01.T01:exon:2577;Parent=MsG0780036722.01.T01 |
| Chr7 | exon | 12481803 | 12482177 | 12481803 | ID=MsG0780036722.01.T01:exon:2578;Parent=MsG0780036722.01.T01 |
| Chr7 | five_prime_UTR | 12481323 | 12481411 | 12481323 | ID=MsG0780036722.01.T01:five_prime_utr;Parent=MsG0780036722.01.T01 |
| Chr7 | CDS | 12481412 | 12481513 | 12481412 | ID=MsG0780036722.01.T01:cds;Parent=MsG0780036722.01.T01 |
| Chr7 | CDS | 12481803 | 12482018 | 12481803 | ID=MsG0780036722.01.T01:cds;Parent=MsG0780036722.01.T01 |
| Chr7 | three_prime_UTR | 12482019 | 12482177 | 12482019 | ID=MsG0780036722.01.T01:three_prime_utr;Parent=MsG0780036722.01.T01 |
| Gene Sequence |
| Protein sequence |
