AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038377.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038377.01.T01 MTR_7g052820 84.416 77 3 1 3 79 542 609 1.05e-36 129
MsG0780038377.01.T01 MTR_8g011460 80.519 77 6 1 3 79 540 607 6.34e-35 124
MsG0780038377.01.T01 MTR_5g098130 67.532 77 16 1 3 79 539 606 1.75e-28 106
MsG0780038377.01.T01 MTR_5g094160 67.532 77 16 1 3 79 539 606 2.45e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038377.01.T01 AT5G48460 75.325 77 10 1 3 79 541 608 2.27e-32 117
MsG0780038377.01.T01 AT2G04750 66.234 77 17 1 3 79 535 602 1.14e-27 104
MsG0780038377.01.T01 AT4G26700 64.935 77 18 1 3 79 390 457 3.19e-27 102
MsG0780038377.01.T01 AT5G35700 64.935 77 18 1 3 79 539 606 4.26e-27 102
MsG0780038377.01.T01 AT4G26700 64.935 77 18 1 3 79 539 606 4.93e-27 102
MsG0780038377.01.T01 AT4G26700 64.935 77 18 1 3 79 539 606 4.93e-27 102
MsG0780038377.01.T01 AT4G26700 64.935 77 18 1 3 79 539 606 4.93e-27 102
MsG0780038377.01.T01 AT5G55400 63.636 77 19 1 3 79 540 607 6.77e-27 102
MsG0780038377.01.T01 AT5G55400 63.636 77 19 1 3 79 540 607 6.77e-27 102
MsG0780038377.01.T01 AT5G55400 63.636 77 19 1 3 79 540 607 6.77e-27 102
MsG0780038377.01.T01 AT4G26700 68.421 38 12 0 3 40 539 576 1.24e-11 58.9
MsG0780038377.01.T01 AT4G26700 68.421 38 12 0 3 40 539 576 1.24e-11 58.9
MsG0780038377.01.T01 AT4G26700 68.421 38 12 0 3 40 539 576 1.24e-11 58.9

Find 14 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAATTGTTTGAAATTTC+AGG 0.184471 7:-45453059 None:intergenic
CTGCATAATTTGATAAATAA+TGG 0.249580 7:+45452085 None:intergenic
TACAACCTAGAGCTGTGAAT+TGG 0.353089 7:-45452988 MsG0780038377.01.T01:CDS
ACAACCTAGAGCTGTGAATT+GGG 0.394703 7:-45452987 MsG0780038377.01.T01:CDS
ATCAGTATTGCAAGAAAACT+TGG 0.410928 7:-45452030 MsG0780038377.01.T01:CDS
ACTACCTCAGTGATGTCTTC+AGG 0.412757 7:+45451988 None:intergenic
CTTGTGACAAAAGGAGTAAC+TGG 0.423464 7:-45452962 MsG0780038377.01.T01:intron
TCTTGCAATACTGATAATGT+AGG 0.492990 7:+45452038 None:intergenic
CAGGATAAAAGTTTATCAGA+TGG 0.519978 7:-45453040 MsG0780038377.01.T01:CDS
CTCTAGGTTGTACAGAACTA+AGG 0.521248 7:+45452999 None:intergenic
AGACCCCAATTCACAGCTCT+AGG 0.587212 7:+45452983 None:intergenic
AATTGGGGTCTTGTGACAAA+AGG 0.587266 7:-45452971 MsG0780038377.01.T01:CDS
CAACCTAGAGCTGTGAATTG+GGG 0.619563 7:-45452986 MsG0780038377.01.T01:CDS
GCTTCCTGAAGACATCACTG+AGG 0.793102 7:-45451992 MsG0780038377.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATCTCTAAAAAATAT+GGG + Chr7:45452251-45452270 None:intergenic 10.0%
!!! TAAAAAATCTCTAAAAAATA+TGG + Chr7:45452252-45452271 None:intergenic 10.0%
!! TACATATAAAAAAAAGAATC+TGG + Chr7:45452133-45452152 None:intergenic 15.0%
!!! AAATACTTTATTTGAAAACA+TGG - Chr7:45452904-45452923 MsG0780038377.01.T01:intron 15.0%
!!! ATTGATTATTGTTTTTTTGA+GGG + Chr7:45452605-45452624 None:intergenic 15.0%
!!! ATTTTATTGTTTGTTTCATA+TGG + Chr7:45452883-45452902 None:intergenic 15.0%
!! AAACAAAGAATAAAAAGTTG+TGG + Chr7:45452193-45452212 None:intergenic 20.0%
!! AAATTAGTCATCACTAAAAT+CGG + Chr7:45452672-45452691 None:intergenic 20.0%
!! CTGCATAATTTGATAAATAA+TGG + Chr7:45452942-45452961 None:intergenic 20.0%
!! TAAAAAAAAGAATCTGGAAA+AGG + Chr7:45452127-45452146 None:intergenic 20.0%
!!! CATTGATTATTGTTTTTTTG+AGG + Chr7:45452606-45452625 None:intergenic 20.0%
! AAAGAATAAAAAGTTGTGGA+GGG + Chr7:45452189-45452208 None:intergenic 25.0%
! AATTTGACTGACCATATTTA+TGG - Chr7:45452700-45452719 MsG0780038377.01.T01:intron 25.0%
! ATCATAGTTACCAAAACTAA+AGG + Chr7:45452631-45452650 None:intergenic 25.0%
! TCAAAGATTCAACAAGTAAA+AGG + Chr7:45452096-45452115 None:intergenic 25.0%
!! GTATTATTCCTGTCATATTT+TGG - Chr7:45452499-45452518 MsG0780038377.01.T01:intron 25.0%
!! TAGAAAAAGTGTATTTAAGC+CGG + Chr7:45452733-45452752 None:intergenic 25.0%
!!! AAAAAGCAAGCTGTTTTAAA+TGG + Chr7:45452368-45452387 None:intergenic 25.0%
!!! AATCAATGATCCTTTAGTTT+TGG - Chr7:45452618-45452637 MsG0780038377.01.T01:intron 25.0%
!!! AGTTTTGGTAACTATGATAT+TGG - Chr7:45452633-45452652 MsG0780038377.01.T01:intron 25.0%
!!! ATACACTTTTTCTATTTGCA+AGG - Chr7:45452739-45452758 MsG0780038377.01.T01:intron 25.0%
!!! ATATTTTGGTTGTGATACAT+GGG - Chr7:45452513-45452532 MsG0780038377.01.T01:intron 25.0%
!!! ATCAGATGGAATATTTTTTC+TGG - Chr7:45451998-45452017 MsG0780038377.01.T01:CDS 25.0%
AATCATGAAATTCTCTCAAG+TGG - Chr7:45452834-45452853 MsG0780038377.01.T01:intron 30.0%
ATCAGTATTGCAAGAAAACT+TGG - Chr7:45452994-45453013 MsG0780038377.01.T01:CDS 30.0%
ATCATGAAATTCTCTCAAGT+GGG - Chr7:45452835-45452854 MsG0780038377.01.T01:intron 30.0%
CAAAGAATAAAAAGTTGTGG+AGG + Chr7:45452190-45452209 None:intergenic 30.0%
CAAGTTTATAAAAGCCATCA+TGG - Chr7:45452472-45452491 MsG0780038377.01.T01:intron 30.0%
CAGGATAAAAGTTTATCAGA+TGG - Chr7:45451984-45452003 MsG0780038377.01.T01:CDS 30.0%
TATCACAACCAAAATATGAC+AGG + Chr7:45452510-45452529 None:intergenic 30.0%
TATGCTTTCAAACAGTTAGA+AGG + Chr7:45452412-45452431 None:intergenic 30.0%
! AGAAAAAGTGTATTTAAGCC+GGG + Chr7:45452732-45452751 None:intergenic 30.0%
! TACTTGTTGAATCTTTGACT+TGG - Chr7:45452098-45452117 MsG0780038377.01.T01:intron 30.0%
! TCTTGCAATACTGATAATGT+AGG + Chr7:45452989-45453008 None:intergenic 30.0%
!!! CATATTTTGGTTGTGATACA+TGG - Chr7:45452512-45452531 MsG0780038377.01.T01:intron 30.0%
!!! TATTGTTTTTTTGAGGGAGA+AGG + Chr7:45452599-45452618 None:intergenic 30.0%
GGAATAATACAAGACCATGA+TGG + Chr7:45452489-45452508 None:intergenic 35.0%
TCATGAAATTCTCTCAAGTG+GGG - Chr7:45452836-45452855 MsG0780038377.01.T01:intron 35.0%
! GAAAAAGTGTATTTAAGCCG+GGG + Chr7:45452731-45452750 None:intergenic 35.0%
!! ACTCTAACCAAAGCTTTAGA+AGG + Chr7:45452785-45452804 None:intergenic 35.0%
ACAACCTAGAGCTGTGAATT+GGG - Chr7:45452037-45452056 MsG0780038377.01.T01:CDS 40.0%
CTCTAGGTTGTACAGAACTA+AGG + Chr7:45452028-45452047 None:intergenic 40.0%
CTTGTGACAAAAGGAGTAAC+TGG - Chr7:45452062-45452081 MsG0780038377.01.T01:CDS 40.0%
TACAACCTAGAGCTGTGAAT+TGG - Chr7:45452036-45452055 MsG0780038377.01.T01:CDS 40.0%
! AATTGGGGTCTTGTGACAAA+AGG - Chr7:45452053-45452072 MsG0780038377.01.T01:CDS 40.0%
! CTTTAGAAGGAACAAGGTCT+CGG + Chr7:45452772-45452791 None:intergenic 40.0%
!! CCAAAGCTTTAGAAGGAACA+AGG + Chr7:45452778-45452797 None:intergenic 40.0%
!! CCTTGTTCCTTCTAAAGCTT+TGG - Chr7:45452775-45452794 MsG0780038377.01.T01:intron 40.0%
ACTACCTCAGTGATGTCTTC+AGG + Chr7:45453039-45453058 None:intergenic 45.0%
CAACCTAGAGCTGTGAATTG+GGG - Chr7:45452038-45452057 MsG0780038377.01.T01:CDS 45.0%
CCATATTTATGGTGTCTCCC+CGG - Chr7:45452711-45452730 MsG0780038377.01.T01:intron 45.0%
AGACCCCAATTCACAGCTCT+AGG + Chr7:45452044-45452063 None:intergenic 50.0%
CCGGGGAGACACCATAAATA+TGG + Chr7:45452714-45452733 None:intergenic 50.0%
GCTTCCTGAAGACATCACTG+AGG - Chr7:45453032-45453051 MsG0780038377.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 45451975 45453071 45451975 ID=MsG0780038377.01;Name=MsG0780038377.01
Chr7 mRNA 45451975 45453071 45451975 ID=MsG0780038377.01.T01;Parent=MsG0780038377.01;Name=MsG0780038377.01.T01;_AED=0.50;_eAED=0.58;_QI=0|0|0|1|0|0|2|0|81
Chr7 exon 45452963 45453071 45452963 ID=MsG0780038377.01.T01:exon:5161;Parent=MsG0780038377.01.T01
Chr7 exon 45451975 45452111 45451975 ID=MsG0780038377.01.T01:exon:5160;Parent=MsG0780038377.01.T01
Chr7 CDS 45452963 45453071 45452963 ID=MsG0780038377.01.T01:cds;Parent=MsG0780038377.01.T01
Chr7 CDS 45451975 45452111 45451975 ID=MsG0780038377.01.T01:cds;Parent=MsG0780038377.01.T01
Gene Sequence

>MsG0780038377.01.T01

TTGAAATTTCAGGATAAAAGTTTATCAGATGGAATATTTTTTCTGGAGCTCCTTAGTTCTGTACAACCTAGAGCTGTGAATTGGGGTCTTGTGACAAAAGGAGTAACTGCAAACCATTATTTATCAAATTATGCAGATCAAGAGAAAATGATGAATGCTACCTACATTATCAGTATTGCAAGAAAACTTGGTTGCTCAATATTCTTGCTTCCTGAAGACATCACTGAGGTAGTTCAAATTTTGTAA

Protein sequence

>MsG0780038377.01.T01

LKFQDKSLSDGIFFLELLSSVQPRAVNWGLVTKGVTANHYLSNYADQEKMMNATYIISIARKLGCSIFLLPEDITEVVQIL*