Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038735.01.T01 | XP_013448846.1 | 94.872 | 78 | 4 | 0 | 1 | 78 | 1 | 78 | 6.77E-27 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038735.01.T01 | Q0IMG5 | 58.75 | 80 | 28 | 2 | 1 | 77 | 1 | 78 | 3.07E-22 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038735.01.T01 | A0A072U177 | 94.872 | 78 | 4 | 0 | 1 | 78 | 1 | 78 | 3.23e-27 | 103 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048197.01 | MsG0780038735.01 | 0.807455 | 5.012968e-50 | 2.793457e-47 |
| MsG0380012073.01 | MsG0780038735.01 | 0.804409 | 2.190589e-49 | 1.128588e-46 |
| MsG0380014137.01 | MsG0780038735.01 | -0.820516 | 6.581902e-53 | 5.218855e-50 |
| MsG0480021464.01 | MsG0780038735.01 | 0.807000 | 6.259889e-50 | 3.447088e-47 |
| MsG0480021842.01 | MsG0780038735.01 | 0.825692 | 4.079928e-54 | 3.745910e-51 |
| MsG0480022614.01 | MsG0780038735.01 | 0.807671 | 4.511435e-50 | 2.527971e-47 |
| MsG0480022790.01 | MsG0780038735.01 | 0.806500 | 7.983573e-50 | 4.340021e-47 |
| MsG0480023771.01 | MsG0780038735.01 | 0.806165 | 9.392911e-50 | 5.062966e-47 |
| MsG0380017382.01 | MsG0780038735.01 | 0.817758 | 2.793138e-52 | 2.052067e-49 |
| MsG0680030408.01 | MsG0780038735.01 | 0.816170 | 6.349506e-52 | 4.466288e-49 |
| MsG0780037590.01 | MsG0780038735.01 | -0.801001 | 1.106370e-48 | 5.226043e-46 |
| MsG0780038735.01 | MsG0880043477.01 | 0.805137 | 1.543581e-49 | 8.103522e-47 |
| MsG0780038735.01 | MsG0880045951.01 | 0.814539 | 1.463616e-51 | 9.847569e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038735.01.T01 | MTR_7g061018 | 94.872 | 78 | 4 | 0 | 1 | 78 | 1 | 78 | 8.20e-31 | 103 |
| MsG0780038735.01.T01 | MTR_4g021730 | 55.405 | 74 | 30 | 2 | 7 | 78 | 3 | 75 | 1.21e-17 | 70.1 |
| MsG0780038735.01.T01 | MTR_4g021725 | 53.247 | 77 | 27 | 3 | 4 | 78 | 6 | 75 | 2.20e-15 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038735.01.T01 | AT3G09390 | 70.370 | 81 | 21 | 3 | 1 | 78 | 1 | 81 | 1.07e-11 | 55.5 |
| MsG0780038735.01.T01 | AT3G09390 | 70.370 | 81 | 21 | 3 | 1 | 78 | 1 | 81 | 1.07e-11 | 55.5 |
| MsG0780038735.01.T01 | AT5G02380 | 70.000 | 80 | 19 | 3 | 1 | 78 | 1 | 77 | 9.94e-11 | 52.8 |
Find 39 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTAATGAAATAGTATTAT+TGG | 0.126803 | 7:+52079663 | MsG0780038735.01.T01:three_prime_UTR |
| TGATAAGTGTGCTTTAACTA+TGG | 0.256492 | 7:+52079599 | MsG0780038735.01.T01:three_prime_UTR |
| TAAGTTATTATCATCACTAA+TGG | 0.278002 | 7:-52079447 | None:intergenic |
| TTTCAGCAGCATCCTCGAAT+TGG | 0.328362 | 7:-52079318 | None:intergenic |
| TTCAGCAGCATCCTCGAATT+GGG | 0.335137 | 7:-52079317 | None:intergenic |
| ACAGCCAGTGCCGCATTTGC+AGG | 0.352772 | 7:-52078515 | None:intergenic |
| TGCAAATGCGGCACTGGCTG+TGG | 0.361672 | 7:+52078517 | MsG0780038735.01.T01:CDS |
| GATTCTGTGTAGCTCAAATC+AGG | 0.372420 | 7:-52079250 | None:intergenic |
| TTACACTCTAAAATAGTGTT+TGG | 0.379354 | 7:-52079532 | None:intergenic |
| CAGGTTCATTTGCAAGTGCA+TGG | 0.385728 | 7:-52079391 | None:intergenic |
| AGCATCCTCGAATTGGGGTT+TGG | 0.389589 | 7:-52079311 | None:intergenic |
| ATTCGAGGATGCTGCTGAAA+TGG | 0.399468 | 7:+52079321 | MsG0780038735.01.T01:CDS |
| AAGTTATTATCATCACTAAT+GGG | 0.419512 | 7:-52079446 | None:intergenic |
| AGTGCCTGCAAATGCGGCAC+TGG | 0.424055 | 7:+52078511 | MsG0780038735.01.T01:CDS |
| CTGAAAATGTCTTGCTGTGG+TGG | 0.438298 | 7:+52078472 | MsG0780038735.01.T01:exon |
| CATGGGTTGCAGCTGCAGTT+TGG | 0.448219 | 7:-52079373 | None:intergenic |
| TTTATGTGAACTCTAGTGTT+TGG | 0.452157 | 7:+52079501 | MsG0780038735.01.T01:three_prime_UTR |
| AGTATTATTGGAAAATTAAG+TGG | 0.456437 | 7:+52079675 | MsG0780038735.01.T01:three_prime_UTR |
| CATTAATAAGCAGAGATTGA+AGG | 0.463732 | 7:-52079648 | None:intergenic |
| ATGAGACTTTGAAGCAGAGA+TGG | 0.476193 | 7:+52079417 | MsG0780038735.01.T01:three_prime_UTR |
| TTCGAGGATGCTGCTGAAAT+GGG | 0.488607 | 7:+52079322 | MsG0780038735.01.T01:CDS |
| AGGTTCATTTGCAAGTGCAT+GGG | 0.494035 | 7:-52079390 | None:intergenic |
| AAATGCGGCACTGGCTGTGG+AGG | 0.501749 | 7:+52078520 | MsG0780038735.01.T01:CDS |
| ATCTGCCAAACCCCAATTCG+AGG | 0.505527 | 7:+52079306 | MsG0780038735.01.T01:CDS |
| AACCACTGAGACCCTTGTGA+TGG | 0.567822 | 7:+52079276 | MsG0780038735.01.T01:CDS |
| ACCACTGAGACCCTTGTGAT+GGG | 0.567834 | 7:+52079277 | MsG0780038735.01.T01:CDS |
| TGAGACTTTGAAGCAGAGAT+GGG | 0.569056 | 7:+52079418 | MsG0780038735.01.T01:three_prime_UTR |
| TCAGCAGCATCCTCGAATTG+GGG | 0.581589 | 7:-52079316 | None:intergenic |
| GAGAATGATGGCTGCAAGTG+TGG | 0.583748 | 7:+52079349 | MsG0780038735.01.T01:CDS |
| CAGATGCAACTCCCATCACA+AGG | 0.587599 | 7:-52079288 | None:intergenic |
| TCTTGCTGTGGTGGAAACTG+TGG | 0.591533 | 7:+52078481 | MsG0780038735.01.T01:CDS |
| TGTGGTGGAAACTGTGGTTG+TGG | 0.595875 | 7:+52078487 | MsG0780038735.01.T01:CDS |
| TGTGGAAGTGCCTGCAAATG+CGG | 0.600913 | 7:+52078505 | MsG0780038735.01.T01:CDS |
| GAAATGGGTGCTGAGAATGA+TGG | 0.610124 | 7:+52079337 | MsG0780038735.01.T01:CDS |
| TCCCATCACAAGGGTCTCAG+TGG | 0.626453 | 7:-52079278 | None:intergenic |
| AATGCGGCACTGGCTGTGGA+GGG | 0.639645 | 7:+52078521 | MsG0780038735.01.T01:CDS |
| ATTCTGTGTAGCTCAAATCA+GGG | 0.645627 | 7:-52079249 | None:intergenic |
| CATCTGAAAATGTCTTGCTG+TGG | 0.660819 | 7:+52078469 | MsG0780038735.01.T01:exon |
| AGATGCAACTCCCATCACAA+GGG | 0.706946 | 7:-52079287 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTAATGAAATAGTATTAT+TGG | + | Chr7:52079663-52079682 | MsG0780038735.01.T01:three_prime_UTR | 10.0% |
| !!! | TTAATTCATTGATTATTTTA+GGG | + | Chr7:52078855-52078874 | MsG0780038735.01.T01:intron | 10.0% |
| !!! | TTTAATTCATTGATTATTTT+AGG | + | Chr7:52078854-52078873 | MsG0780038735.01.T01:intron | 10.0% |
| !! | TAAAATTTAACTAAACCAAA+GGG | - | Chr7:52078886-52078905 | None:intergenic | 15.0% |
| !! | TAATTTGACAAAATATTTCT+AGG | + | Chr7:52079140-52079159 | MsG0780038735.01.T01:intron | 15.0% |
| !! | TTAAAATTTAACTAAACCAA+AGG | - | Chr7:52078887-52078906 | None:intergenic | 15.0% |
| !!! | CTCTCTTTTTTTTTTTTTTT+TGG | + | Chr7:52078562-52078581 | MsG0780038735.01.T01:intron | 15.0% |
| !!! | GAAATTTTTAAGTAGAAAAA+AGG | - | Chr7:52079113-52079132 | None:intergenic | 15.0% |
| !!! | TAATTCATTGATTATTTTAG+GGG | + | Chr7:52078856-52078875 | MsG0780038735.01.T01:intron | 15.0% |
| !!! | TTCTTTTGATTTGATTTTTT+TGG | + | Chr7:52078643-52078662 | MsG0780038735.01.T01:intron | 15.0% |
| !! | AAAATTTAACTAAACCAAAG+GGG | - | Chr7:52078885-52078904 | None:intergenic | 20.0% |
| !! | AAGTTATTATCATCACTAAT+GGG | - | Chr7:52079449-52079468 | None:intergenic | 20.0% |
| !! | AATTCTTCAAATTCAATTAG+AGG | - | Chr7:52078826-52078845 | None:intergenic | 20.0% |
| !! | AGTATTATTGGAAAATTAAG+TGG | + | Chr7:52079675-52079694 | MsG0780038735.01.T01:three_prime_UTR | 20.0% |
| !! | TAAGTTATTATCATCACTAA+TGG | - | Chr7:52079450-52079469 | None:intergenic | 20.0% |
| !!! | TGTATTTTTCATTTGCTTTA+TGG | + | Chr7:52079015-52079034 | MsG0780038735.01.T01:intron | 20.0% |
| !!! | TTTTGTGTAAATTGTTTCAT+GGG | + | Chr7:52078778-52078797 | MsG0780038735.01.T01:intron | 20.0% |
| !!! | TTTTTGTGTAAATTGTTTCA+TGG | + | Chr7:52078777-52078796 | MsG0780038735.01.T01:intron | 20.0% |
| ! | ACTATGGTTGTAAAAATGTT+CGG | + | Chr7:52079615-52079634 | MsG0780038735.01.T01:three_prime_UTR | 25.0% |
| ! | TATCACAAAAATTGCTCAAA+GGG | - | Chr7:52079584-52079603 | None:intergenic | 25.0% |
| ! | TTATCACAAAAATTGCTCAA+AGG | - | Chr7:52079585-52079604 | None:intergenic | 25.0% |
| ! | TTTGTGTAAATTGTTTCATG+GGG | + | Chr7:52078779-52078798 | MsG0780038735.01.T01:intron | 25.0% |
| !! | AGTCAATTGTTGATTAAGAA+GGG | - | Chr7:52079050-52079069 | None:intergenic | 25.0% |
| !! | TAGTCAATTGTTGATTAAGA+AGG | - | Chr7:52079051-52079070 | None:intergenic | 25.0% |
| !!! | TTACACTCTAAAATAGTGTT+TGG | - | Chr7:52079535-52079554 | None:intergenic | 25.0% |
| AACAAAACCTCAAAAGCTAA+TGG | - | Chr7:52078965-52078984 | None:intergenic | 30.0% | |
| AAGAAACAGAAAACTTTCTC+TGG | - | Chr7:52078628-52078647 | None:intergenic | 30.0% | |
| CAATTTCACAAAGACAAAGA+TGG | + | Chr7:52078985-52079004 | MsG0780038735.01.T01:intron | 30.0% | |
| CATTAATAAGCAGAGATTGA+AGG | - | Chr7:52079651-52079670 | None:intergenic | 30.0% | |
| TCCATGCAATTCATCAAAAA+AGG | - | Chr7:52078671-52078690 | None:intergenic | 30.0% | |
| TGATAAGTGTGCTTTAACTA+TGG | + | Chr7:52079599-52079618 | MsG0780038735.01.T01:three_prime_UTR | 30.0% | |
| TTAACCATTCCTCTTATGTT+GGG | + | Chr7:52078705-52078724 | MsG0780038735.01.T01:intron | 30.0% | |
| TTTAACCATTCCTCTTATGT+TGG | + | Chr7:52078704-52078723 | MsG0780038735.01.T01:intron | 30.0% | |
| TTTATGTGAACTCTAGTGTT+TGG | + | Chr7:52079501-52079520 | MsG0780038735.01.T01:three_prime_UTR | 30.0% | |
| TTTCATTTGCTTTATGGCTT+TGG | + | Chr7:52079021-52079040 | MsG0780038735.01.T01:intron | 30.0% | |
| ! | GCTACGTTTCAATTTTGTAT+TGG | + | Chr7:52079087-52079106 | MsG0780038735.01.T01:intron | 30.0% |
| !!! | ACCTTTTTTGATGAATTGCA+TGG | + | Chr7:52078667-52078686 | MsG0780038735.01.T01:intron | 30.0% |
| ATTCTGTGTAGCTCAAATCA+GGG | - | Chr7:52079252-52079271 | None:intergenic | 35.0% | |
| ! | CACAATTTTCCCAACATAAG+AGG | - | Chr7:52078717-52078736 | None:intergenic | 35.0% |
| ! | TGCTTCAAAGTCTCATTTTC+AGG | - | Chr7:52079413-52079432 | None:intergenic | 35.0% |
| ! | TTTTCCCAACATAAGAGGAA+TGG | - | Chr7:52078712-52078731 | None:intergenic | 35.0% |
| !! | CACTTATTTTCTGTTGTGTG+AGG | + | Chr7:52078744-52078763 | MsG0780038735.01.T01:intron | 35.0% |
| !!! | TGAGATTCCATTAGCTTTTG+AGG | + | Chr7:52078955-52078974 | MsG0780038735.01.T01:intron | 35.0% |
| AGGTTCATTTGCAAGTGCAT+GGG | - | Chr7:52079393-52079412 | None:intergenic | 40.0% | |
| CATCTGAAAATGTCTTGCTG+TGG | + | Chr7:52078469-52078488 | MsG0780038735.01.T01:exon | 40.0% | |
| GATTCTGTGTAGCTCAAATC+AGG | - | Chr7:52079253-52079272 | None:intergenic | 40.0% | |
| !! | ATGAGACTTTGAAGCAGAGA+TGG | + | Chr7:52079417-52079436 | MsG0780038735.01.T01:three_prime_UTR | 40.0% |
| !! | TGAGACTTTGAAGCAGAGAT+GGG | + | Chr7:52079418-52079437 | MsG0780038735.01.T01:three_prime_UTR | 40.0% |
| !!! | TATTTTAGGGGTATCCCCTT+TGG | + | Chr7:52078868-52078887 | MsG0780038735.01.T01:intron | 40.0% |
| AGATGCAACTCCCATCACAA+GGG | - | Chr7:52079290-52079309 | None:intergenic | 45.0% | |
| ATTCGAGGATGCTGCTGAAA+TGG | + | Chr7:52079321-52079340 | MsG0780038735.01.T01:CDS | 45.0% | |
| CAGGTTCATTTGCAAGTGCA+TGG | - | Chr7:52079394-52079413 | None:intergenic | 45.0% | |
| TTCAGCAGCATCCTCGAATT+GGG | - | Chr7:52079320-52079339 | None:intergenic | 45.0% | |
| TTCGAGGATGCTGCTGAAAT+GGG | + | Chr7:52079322-52079341 | MsG0780038735.01.T01:CDS | 45.0% | |
| TTTCAGCAGCATCCTCGAAT+TGG | - | Chr7:52079321-52079340 | None:intergenic | 45.0% | |
| ! | CTGAAAATGTCTTGCTGTGG+TGG | + | Chr7:52078472-52078491 | MsG0780038735.01.T01:exon | 45.0% |
| !! | GAAATGGGTGCTGAGAATGA+TGG | + | Chr7:52079337-52079356 | MsG0780038735.01.T01:CDS | 45.0% |
| AACCACTGAGACCCTTGTGA+TGG | + | Chr7:52079276-52079295 | MsG0780038735.01.T01:CDS | 50.0% | |
| ACCACTGAGACCCTTGTGAT+GGG | + | Chr7:52079277-52079296 | MsG0780038735.01.T01:CDS | 50.0% | |
| AGCATCCTCGAATTGGGGTT+TGG | - | Chr7:52079314-52079333 | None:intergenic | 50.0% | |
| ATCTGCCAAACCCCAATTCG+AGG | + | Chr7:52079306-52079325 | MsG0780038735.01.T01:CDS | 50.0% | |
| CAGATGCAACTCCCATCACA+AGG | - | Chr7:52079291-52079310 | None:intergenic | 50.0% | |
| GAGAATGATGGCTGCAAGTG+TGG | + | Chr7:52079349-52079368 | MsG0780038735.01.T01:CDS | 50.0% | |
| TCAGCAGCATCCTCGAATTG+GGG | - | Chr7:52079319-52079338 | None:intergenic | 50.0% | |
| ! | TCTTGCTGTGGTGGAAACTG+TGG | + | Chr7:52078481-52078500 | MsG0780038735.01.T01:CDS | 50.0% |
| ! | TGTGGTGGAAACTGTGGTTG+TGG | + | Chr7:52078487-52078506 | MsG0780038735.01.T01:CDS | 50.0% |
| !! | TGTGGAAGTGCCTGCAAATG+CGG | + | Chr7:52078505-52078524 | MsG0780038735.01.T01:CDS | 50.0% |
| TCCCATCACAAGGGTCTCAG+TGG | - | Chr7:52079281-52079300 | None:intergenic | 55.0% | |
| ! | CATGGGTTGCAGCTGCAGTT+TGG | - | Chr7:52079376-52079395 | None:intergenic | 55.0% |
| ACAGCCAGTGCCGCATTTGC+AGG | - | Chr7:52078518-52078537 | None:intergenic | 60.0% | |
| AGTGCCTGCAAATGCGGCAC+TGG | + | Chr7:52078511-52078530 | MsG0780038735.01.T01:CDS | 60.0% | |
| !! | AAATGCGGCACTGGCTGTGG+AGG | + | Chr7:52078520-52078539 | MsG0780038735.01.T01:CDS | 60.0% |
| !! | AATGCGGCACTGGCTGTGGA+GGG | + | Chr7:52078521-52078540 | MsG0780038735.01.T01:CDS | 60.0% |
| !! | TGCAAATGCGGCACTGGCTG+TGG | + | Chr7:52078517-52078536 | MsG0780038735.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 52078463 | 52079697 | 52078463 | ID=MsG0780038735.01;Name=MsG0780038735.01 |
| Chr7 | mRNA | 52078463 | 52079697 | 52078463 | ID=MsG0780038735.01.T01;Parent=MsG0780038735.01;Name=MsG0780038735.01.T01;_AED=0.50;_eAED=0.50;_QI=15|1|1|1|0|0|2|289|78 |
| Chr7 | exon | 52078463 | 52078542 | 52078463 | ID=MsG0780038735.01.T01:exon:8258;Parent=MsG0780038735.01.T01 |
| Chr7 | exon | 52079237 | 52079697 | 52079237 | ID=MsG0780038735.01.T01:exon:8259;Parent=MsG0780038735.01.T01 |
| Chr7 | five_prime_UTR | 52078463 | 52078477 | 52078463 | ID=MsG0780038735.01.T01:five_prime_utr;Parent=MsG0780038735.01.T01 |
| Chr7 | CDS | 52078478 | 52078542 | 52078478 | ID=MsG0780038735.01.T01:cds;Parent=MsG0780038735.01.T01 |
| Chr7 | CDS | 52079237 | 52079408 | 52079237 | ID=MsG0780038735.01.T01:cds;Parent=MsG0780038735.01.T01 |
| Chr7 | three_prime_UTR | 52079409 | 52079697 | 52079409 | ID=MsG0780038735.01.T01:three_prime_utr;Parent=MsG0780038735.01.T01 |
| Gene Sequence |
| Protein sequence |
